WO2011155799A3 - 탄화수소 생성능을 가지는 변이 미생물 및 이를 이용한 탄화수소의 제조방법 - Google Patents

탄화수소 생성능을 가지는 변이 미생물 및 이를 이용한 탄화수소의 제조방법 Download PDF

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WO2011155799A3
WO2011155799A3 PCT/KR2011/004289 KR2011004289W WO2011155799A3 WO 2011155799 A3 WO2011155799 A3 WO 2011155799A3 KR 2011004289 W KR2011004289 W KR 2011004289W WO 2011155799 A3 WO2011155799 A3 WO 2011155799A3
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hydrocarbon
mutant microorganism
genes
enzymes
code
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PCT/KR2011/004289
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English (en)
French (fr)
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WO2011155799A2 (ko
WO2011155799A9 (ko
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이상엽
최용준
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한국과학기술원
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Priority to EP11792710.3A priority Critical patent/EP2592138B1/en
Priority to CA2802186A priority patent/CA2802186A1/en
Priority to US13/702,920 priority patent/US8895276B2/en
Priority to JP2013514122A priority patent/JP5723977B2/ja
Priority to BR112012031383A priority patent/BR112012031383A2/pt
Priority to CN201180035839.3A priority patent/CN103025864B/zh
Publication of WO2011155799A2 publication Critical patent/WO2011155799A2/ko
Publication of WO2011155799A3 publication Critical patent/WO2011155799A3/ko
Publication of WO2011155799A9 publication Critical patent/WO2011155799A9/ko

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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/002—Preparation of hydrocarbons or halogenated hydrocarbons cyclic
    • C12P5/005—Preparation of hydrocarbons or halogenated hydrocarbons cyclic aromatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes

Abstract

본 발명은 알칸을 포함하는 탄화수소 생성능을 가지는 변이 미생물 및 이를 이용한 알칸을 포함하는 탄화수소의 제조방법에 관한 것으로서, 더욱 상세하게는 알칸을 포함하는 탄화수소 생산에 적합하도록 개량된 미생물에 fatty acyl-acp를 free fatty acid로 전환시키는 효소를 코딩하는 유전자, free fatty acid를 fatty acyl-CoA로 전환시키는 효소를 코딩하는 유전자, fatty acyl-CoA를 fatty aldehyde로 전환시키는 효소를 코딩하는 유전자 및 fatty aldehyde를 alkane으로 전환시키는 효소를 코딩하는 유전자가 도입된 변이 미생물 및 이를 이용하는 것을 특징으로 하는 알칸을 포함하는 탄화수소의 제조방법에 관한 것이다. 본 발명에 따른 변이 미생물은 추가적인 대사흐름 조작으로 인한 균주개량에 활용될 가능성이 크므로 알칸을 포함하는 탄화수소의 산업적 생산에 유용하다.
PCT/KR2011/004289 2010-06-10 2011-06-10 탄화수소 생성능을 가지는 변이 미생물 및 이를 이용한 탄화수소의 제조방법 WO2011155799A2 (ko)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP11792710.3A EP2592138B1 (en) 2010-06-10 2011-06-10 Mutant microorganism having a hydrocarbon-generating ability and method for producing hydrocarbon using same
CA2802186A CA2802186A1 (en) 2010-06-10 2011-06-10 Microorganism variants having hydrocarbon producing ability and method for producing hydrocarbon using the same
US13/702,920 US8895276B2 (en) 2010-06-10 2011-06-10 Microorganism variants having hydrocarbon producing ability and method for producing hydrocarbon using the same
JP2013514122A JP5723977B2 (ja) 2010-06-10 2011-06-10 炭化水素生成能を有する変異微生物及びこれを利用した炭化水素の製造方法
BR112012031383A BR112012031383A2 (pt) 2010-06-10 2011-06-10 variante de micro-organismo tendo a capacidade de produzir hidrocarbonetos, método para a preparação da variante e método para a produção de hidrocarbonetos utilizando a mesma
CN201180035839.3A CN103025864B (zh) 2010-06-10 2011-06-10 能够产生烃的微生物变体及用其产生烃的方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20100054994 2010-06-10
KR10-2010-0054994 2010-06-10

Publications (3)

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WO2011155799A2 WO2011155799A2 (ko) 2011-12-15
WO2011155799A3 true WO2011155799A3 (ko) 2012-04-19
WO2011155799A9 WO2011155799A9 (ko) 2012-06-21

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Country Status (8)

Country Link
US (1) US8895276B2 (ko)
EP (1) EP2592138B1 (ko)
JP (1) JP5723977B2 (ko)
KR (1) KR101334981B1 (ko)
CN (1) CN103025864B (ko)
BR (1) BR112012031383A2 (ko)
CA (1) CA2802186A1 (ko)
WO (1) WO2011155799A2 (ko)

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JP6189793B2 (ja) * 2014-05-30 2017-08-30 トヨタ自動車株式会社 組換え微生物及び当該組換え微生物を用いた物質製造方法
JP6034332B2 (ja) 2014-05-30 2016-11-30 トヨタ自動車株式会社 組換え微生物及び当該組換え微生物を用いた物質製造方法
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Also Published As

Publication number Publication date
KR20110135355A (ko) 2011-12-16
US20130130344A1 (en) 2013-05-23
US8895276B2 (en) 2014-11-25
EP2592138A4 (en) 2014-03-26
EP2592138B1 (en) 2018-08-08
BR112012031383A2 (pt) 2018-06-19
WO2011155799A2 (ko) 2011-12-15
CA2802186A1 (en) 2011-12-15
JP2013528057A (ja) 2013-07-08
WO2011155799A9 (ko) 2012-06-21
CN103025864B (zh) 2016-10-12
CN103025864A (zh) 2013-04-03
KR101334981B1 (ko) 2013-12-02
EP2592138A2 (en) 2013-05-15
JP5723977B2 (ja) 2015-05-27

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