WO2011149272A3 - 단백질분해에 의하여 활성화되는 효소의 활성형의 정성적 및 정량적 분석방법 - Google Patents

단백질분해에 의하여 활성화되는 효소의 활성형의 정성적 및 정량적 분석방법 Download PDF

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Publication number
WO2011149272A3
WO2011149272A3 PCT/KR2011/003843 KR2011003843W WO2011149272A3 WO 2011149272 A3 WO2011149272 A3 WO 2011149272A3 KR 2011003843 W KR2011003843 W KR 2011003843W WO 2011149272 A3 WO2011149272 A3 WO 2011149272A3
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Prior art keywords
enzyme
present
activity
activity type
analyzing
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PCT/KR2011/003843
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English (en)
French (fr)
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WO2011149272A9 (ko
WO2011149272A2 (ko
Inventor
이창섭
알렉산더 안성수
강민오
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주식회사 나노엔텍
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Priority claimed from KR1020100107640A external-priority patent/KR20120045822A/ko
Application filed by 주식회사 나노엔텍 filed Critical 주식회사 나노엔텍
Priority to EP11786904.0A priority Critical patent/EP2579040A4/en
Priority to US13/699,868 priority patent/US20130071835A1/en
Priority to KR1020127020306A priority patent/KR101255828B1/ko
Publication of WO2011149272A2 publication Critical patent/WO2011149272A2/ko
Publication of WO2011149272A3 publication Critical patent/WO2011149272A3/ko
Publication of WO2011149272A9 publication Critical patent/WO2011149272A9/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 단백질분해에 의하여 활성화되는 효소의 활성형의 정성 및 정량적 분석방법에 관한 것이다. 본 발명은 효소 활성형 억제제 및 결합제를 이용하여 시료내 효소 활성형을 분석함으로써 단순 결합제(예컨대, 항체)를 이용하여 효소 활성형을 분석하는 방법보다 빠르고 정확하게 분석할 수 있는 장점을 가진다. 본 발명은 단순 결합제를 이용하여 효소 활성형을 분석하는 방법보다 작은 장비를 이용하여 복잡하지 않고 간단하게 시료내에 존재하는 효소 활성형을 분석할 수 있다. 본 발명은 타겟 효소 또는 효소의 활성형에 억제제 및 결합제를 동시에 이용함으로써 신속하고 특이적으로 분석할 수 있으며, 이러한 본 발명의 특성을 이용하여 약품(drug)을 고속으로 그리고 대량(high-throughput)으로 스크리닝을 할 수 있다. 또한, 본 발명은 약품 투여 대상체(예컨대, 동물 또는 인간)에서의 약품의 효능 및 투여량에 대한 임상 모니터링을 위한 POCT(Point of care testing)에 적용될 수 있다.
PCT/KR2011/003843 2010-05-25 2011-05-25 단백질분해에 의하여 활성화되는 효소의 활성형의 정성적 및 정량적 분석방법 WO2011149272A2 (ko)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP11786904.0A EP2579040A4 (en) 2010-05-25 2011-05-25 QUALITATIVE AND QUANTITATIVE ANALYSIS METHOD FOR ANALYZING THE ACTIVITY TYPE OF A PROTEOLYST-ENZYME ACTIVATED
US13/699,868 US20130071835A1 (en) 2010-05-25 2011-05-25 Qualitative and Quantitative Analytical Method for Analyzing the Activity Type of an Enzyme that is Activated by Proteolysis
KR1020127020306A KR101255828B1 (ko) 2010-05-25 2011-05-25 단백질분해에 의하여 활성화되는 효소의 활성형의 정성적 및 정량적 분석방법

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2010-0048665 2010-05-25
KR20100048665 2010-05-25
KR10-2010-0107640 2010-11-01
KR1020100107640A KR20120045822A (ko) 2010-11-01 2010-11-01 효소의 활성형의 정성적 및 정량적인 분석방법

Publications (3)

Publication Number Publication Date
WO2011149272A2 WO2011149272A2 (ko) 2011-12-01
WO2011149272A3 true WO2011149272A3 (ko) 2012-05-31
WO2011149272A9 WO2011149272A9 (ko) 2012-07-19

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/003843 WO2011149272A2 (ko) 2010-05-25 2011-05-25 단백질분해에 의하여 활성화되는 효소의 활성형의 정성적 및 정량적 분석방법

Country Status (4)

Country Link
US (1) US20130071835A1 (ko)
EP (1) EP2579040A4 (ko)
KR (1) KR101255828B1 (ko)
WO (1) WO2011149272A2 (ko)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102590509A (zh) * 2012-03-20 2012-07-18 湖南大学 基于无修饰抗体介导纳米金组装检测组蛋白修饰酶活性及筛选其抑制剂的生物传感方法
CN102937631B (zh) * 2012-11-19 2015-12-23 北京理工大学 一种纳米介孔材料固定靶蛋白筛选中药活性成分的方法
CN104360074A (zh) * 2014-11-03 2015-02-18 杨子学 血浆脂蛋白磷脂酶a2的时间分辨荧光免疫检测方法及试剂盒
KR102018249B1 (ko) 2017-04-04 2019-09-10 한양대학교 산학협력단 프로테아제 활성 분석용 프로브 및 이를 이용한 분석 방법
CN112415196A (zh) * 2020-11-25 2021-02-26 刘世杰 一种分析分子活力的系统和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005501217A (ja) * 2000-10-10 2005-01-13 ディベルサ コーポレーション 生体活性または生体分子のハイスループットスクリーニングまたはキャピラリーに基づくスクリーニング
KR20100075455A (ko) * 2007-09-27 2010-07-02 노파르티스 아게 약물 모니터링 분석법

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100722844B1 (ko) 2006-05-30 2007-05-31 이화여자대학교 산학협력단 단일 측정 시스템에서 효소의 활성 및 양을 인 시투측정하기 위한 방법 및 키트
SG175602A1 (en) * 2006-07-05 2011-11-28 Catalyst Biosciences Inc Protease screening methods and proteases identified thereby
US20100104501A1 (en) * 2006-10-16 2010-04-29 Stefan Golz Prss23 as a biomarker, therapeutic and diagnostic target

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005501217A (ja) * 2000-10-10 2005-01-13 ディベルサ コーポレーション 生体活性または生体分子のハイスループットスクリーニングまたはキャピラリーに基づくスクリーニング
KR20100075455A (ko) * 2007-09-27 2010-07-02 노파르티스 아게 약물 모니터링 분석법

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
E.L.E. DE BRUIJNE ET AL.: "The role of thrombin activatable fibrinolysis inhibitor in arterial thrombosis at a young age: the ATTAC study", J. THROMB. HAEMOST., vol. 7, no. 6, 2009, pages 919 - 927, XP055095069 *
JOHN P. HULME ET AL.: "Detecting activated thrombin activatable fibrinolysis inhibitor TAFIa and inactivated TAFIa TAFlai in normal and hemophilia A plasmas", BULL. KOREAN CHEM. SOC., vol. 30, no. 1, 2009, pages 77 - 82, XP055095064 *
See also references of EP2579040A4 *

Also Published As

Publication number Publication date
US20130071835A1 (en) 2013-03-21
KR101255828B1 (ko) 2013-04-24
WO2011149272A9 (ko) 2012-07-19
KR20120114329A (ko) 2012-10-16
EP2579040A2 (en) 2013-04-10
EP2579040A4 (en) 2014-02-12
WO2011149272A2 (ko) 2011-12-01

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