WO2011134326A1 - 一种用于测定纺织品中壳聚糖纤维含量的方法 - Google Patents

一种用于测定纺织品中壳聚糖纤维含量的方法 Download PDF

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WO2011134326A1
WO2011134326A1 PCT/CN2011/071932 CN2011071932W WO2011134326A1 WO 2011134326 A1 WO2011134326 A1 WO 2011134326A1 CN 2011071932 W CN2011071932 W CN 2011071932W WO 2011134326 A1 WO2011134326 A1 WO 2011134326A1
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titration
solution
textile
chitosan
content
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PCT/CN2011/071932
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English (en)
French (fr)
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周家村
胡广敏
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山东华兴纺织集团有限公司
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Priority to US13/643,551 priority Critical patent/US20130122601A1/en
Priority to KR1020127028273A priority patent/KR101431235B1/ko
Priority to GB1220605.8A priority patent/GB2493670B/en
Priority to JP2013506460A priority patent/JP5557124B2/ja
Publication of WO2011134326A1 publication Critical patent/WO2011134326A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/16—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using titration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/82—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
    • G01N21/83—Turbidimetric titration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G01N21/79—Photometric titration
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16C—COMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
    • G16C99/00—Subject matter not provided for in other groups of this subclass
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Definitions

  • the present invention relates to a method for the content of chitosan fibers, and more particularly to a method for the content of chitosan fibers in textiles. Background technique
  • Chitosan is the only positively charged polymer in nature. It has been widely used in chemical, environmental, food, pharmaceutical, cosmetic, and agricultural applications in recent years. Because chitosan has strong antibacterial and hemostatic functions. It is gradually being used in textiles such as underwear and socks. However, there is currently no method for accurately determining the chitosan content of the active ingredient in functional textiles prepared from chitosan. Therefore, how to determine the content of chitosan fiber in textiles is a problem that needs to be solved. It is also a bottleneck limitation of the regulation of chitosan fiber textiles by our national quality supervision department. It has always restricted the development of chitosan fiber in China and needs to be solved urgently. Summary of the invention
  • An object of the present invention is to provide a method for determining the content of chitosan fibers in textiles, and to establish a method for the simple and rapid determination of chitosan fibers in textiles with high sensitivity.
  • colloidal titration is a capacity analysis method for determining the charged groups of polyelectrolytes in aqueous solution.
  • Chitosan dissolves in dilute acid, binds acid molecules on the amino group or binds one to carry a positive charge, forming a positively charged polyelectrolyte.
  • the number of charged genes of chitosan is determined by a charged electrolyte having a chemical structure, molecular weight and concentration. This is the theoretical basis for the determination of the amino group content of chitosan by colloidal titration, and the endpoint of the dropping point is determined by visual inspection.
  • an anionic polyelectrolyte Potassium Polyvinyl Sulfate (PVSK)
  • PVSK Potassium Polyvinyl Sulfate
  • a positively charged blue organic dye toluidine blue is used as an indicator.
  • Toluidine Blue (T. ⁇ ) is used as an indicator because it has a positive charge and does not undergo charge neutralization reaction with chitosan in an acidic solution, but it can be combined with a negatively charged polyethylene sulfate. However, this combination lags behind the reaction between the positive and negative polyelectrolytes. Before the end of the titration, the solution is blue.
  • a method for determining the content of chitosan fibers in a textile comprising the steps of:
  • Step 1 Weigh the constant weight and not more than 2g of the textile to be tested, place it in the Erlenmeyer flask, add 100ml of 1% acetic acid solution to dissolve the chitosan fiber in the textile to be tested, and use it;
  • Step 2 Weigh the solution to be used in step 1 into another Erlenmeyer flask, add deionized water, and then add toluidine blue indicator for use;
  • Step 3 Put the magnetic rotor into the conical flask containing the solution to be used in the second step, and then place the conical flask on the magnetic stirrer; and titrate the polyethylene sulfate titration into the mist while stirring.
  • the solution turned from blue to purplish red, and did not fade within 20 s and appeared flocculent precipitates. That is the end point of the titration, reading the titration volume v l of the potassium persulfate titration solution ;
  • the titration speed is preferably controlled at
  • Step 4 Take the same amount of deionized water as in step 2, perform blank titration in the same manner as above, and read the titration volume V 2 of the polyvinyl potassium sulfate titration solution ;
  • Step 5 Calculate the content of chitosan fibers in the textile as follows:
  • the technical effect of the present invention is that the technical solution of the present invention provides a method for determining the content of chitosan fibers in textiles by a formula, and the error is small, and a simple and rapid determination of textiles with high sensitivity is established.
  • a method of chitosan fiber content is established.
  • W the number of grams of the object to be tested
  • D -D - deacetylation degree of chitosan (fiber) (abbreviated as D -D) g ⁇ "-the content of chitosan fiber in textiles, % detailed description
  • Embodiment 1 is a diagrammatic representation of Embodiment 1:
  • Step 1 Weigh the constant weight chitosan fiber to be tested with one ten thousandth of an electronic balance, and the ratio of chitosan fiber/Newdale viscose/cotton is 10/30/60, shell
  • the degree of deacetylation of the polysaccharide fiber (abbreviated as D ⁇ D is 93%, which is cut into a 100 ml Erlenmeyer flask, and then 100 ml of a 1% acetic acid solution is added to dissolve the chitosan in the textile to be tested.
  • the dissolution process it is preferred to stir the textile to be tested for 4 hours, and then let stand for 0.5 hours, so that the chitosan fibers in the textile to be tested can be sufficiently dissolved in the acetic acid solution.
  • Step 2 Accurately weigh 5g of the solution to be used in step 1 into a 150ml Erlenmeyer flask with one-tenth of an electronic balance, then add 50ml of deionized water, then add 2-3 drops of toluidine blue indicator to be used.
  • the solvent is deionized water.
  • the toluene blue is produced by Shanghai Sinopharm Group Reagent Co., Ltd., and the concentration of the toluidine blue indicator is 0.1%.
  • the solvent is deionized water.
  • Step 3 Put the magnetic rotor into the conical flask containing the solution to be used in the second step, and then place the conical flask on the magnetic stirrer; and titrate the polyethylene sulfate titration into the mist while stirring.
  • the solution is changed from blue to magenta, and does not fade in 20s and appears as a flocculent precipitate, which is the end point of the titration.
  • the titration volume of the potassium persulfate titration solution is 11.23 ml;
  • the titration solution is produced by Japan Wako Pure Chemical Industries Co., Ltd., product name: polyvinyl potassium sulfate titration solution, code No. 162-21655;
  • the titration speed is preferably controlled at 0.03 ml/s, and if the titration speed is too fast, a temporary titration end point often occurs. Elephant.
  • Step 4 Take 50 ml of deionized water, perform blank titration in the same manner as above, and read the titration volume V 2 of the polyvinyl potassium sulfate titration solution is 0.15 ml ;
  • Step 5 Calculate the content of chitosan fibers in the textile as follows:
  • Embodiment 2 is a diagrammatic representation of Embodiment 1:
  • Step 1 Weigh 0.6g of chitosan fiber to be tested with constant weight of one-tenth of an electronic balance.
  • the ratio of chitosan fiber/cotton is 15/85, and the degree of deacetylation of chitosan fiber.
  • deacetylation degree, abbreviated as D*D is 93%, which is cut into a 100 ml Erlenmeyer flask, and then 100 ml of a 1% acetic acid solution is added to dissolve the chitosan fibers in the textile to be tested;
  • the textile to be tested is stirred for 4 hours, and then allowed to stand for 0.5 hours, so that the chitosan fiber in the textile to be tested is more fully dissolved;
  • Step 2 Accurately weigh 5g of the solution to be used in step 1 into a 150ml Erlenmeyer flask with one-tenth of an electronic balance, then add 50ml of deionized water, then add 2-3 drops of toluidine blue indicator to be used.
  • the toluidine blue is produced by Shanghai Sinopharm Group Reagent Co., Ltd., the Shanghai test card, the concentration of toluidine blue indicator is 0.1%, and the solvent is deionized water.
  • Step 3 Put the magnetic rotor into the conical flask containing the solution to be used in the second step, and then place the conical flask on the magnetic stirrer; the potassium persulfate titration solution is stirred while stirring. Instillation, the best speed control is 0.03ml / s, if the titration speed is too fast, there will often be a temporary titration end point; during the titration process, the solution changes from blue to purple, if it does not fade within 20s and appears The flocculent precipitate, that is, the end point of the titration is reached, and the titration volume of the potassium polyvinyl sulfate titration solution is 10.30 ml;
  • the polyvinyl potassium sulfate titration solution is produced by Japan Wako Pure Chemical Industries Co., Ltd., product name: polyvinyl potassium sulfate titration solution, code NO.162-21655;
  • Step 4 Take 50 ml of deionized water, perform blank titration in the same manner as above, and read the titration volume V 2 of the polyvinyl potassium sulfate titration solution is 0.15 ml;
  • Step 5 Calculate the content of chitosan fibers in the textile as follows:
  • W 2 CXfX (V factory V 2 ) X 0.001X203.19X100/ W 3 X (1-D ⁇ D) /D ⁇ D
  • the reason for the above detection examples is the error: First, there is a problem of unevenness in the blended fabric. Second, there is a difference in the endpoint of the titration due to human visual errors. Third, there are differences in reading the instrument scale due to human visual errors.

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  • Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
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  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
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Description

一种用于測定纺织品中壳聚糖纤维含量的方法 技术领域
本发明涉及一种壳聚糖纤维含量方法，尤其涉及一种用于纺织品中 壳聚糖纤维含量的方法。 背景技术
壳聚糖（Chitosan)是自然界中唯一带正电荷的高分子聚合物， 近 年来在化工、 环保、 食品、 医药、 化妆品、 农业等方面得到越来越广泛 的应用。 由于壳聚糖有很强的抗菌作用和止血功能。 目前正逐步应用于 内衣裤、袜等纺织品中。但目前用壳聚糖制备的功能纺织品还没有一个 准确测定其中有效成分壳聚糖含量的方法。 因此， 如何测定纺织品中壳 聚糖纤维含量是一个需要解决的问题，也是我们国家质监部门监管含壳 聚糖纤维纺织品的一个瓶颈限制， 一直制约着我国壳聚糖纤维的发展， 急需解决。 发明内容
本发明的目的是提供一种用于测定纺织品中壳聚糖纤维含量的方 法， 建立一种具有高灵敏度的简便、快速测定纺织品中壳聚糖纤维含量 的方法。
本发明的理论依据在于：胶体滴定法是一种测定水溶液中聚电解质 带电基团的容量分析法。壳聚糖在稀酸中溶解， 在氨基上结合酸分子或 结合 1个 而带上正电荷， 形成带正电荷的聚电解质。
聚电解质在水溶液中遇到带有相反电荷的聚电解质时，彼此之间会 以一定的化学计量关系发生电中和反应， 形成中性的缔合物， 最终形成 沉淀。
利用化学结构、分子量和浓度已知的带电荷的电解质来测定壳聚糖 的带电基因数量， 这就是胶体滴定法测定壳聚糖的氨基含量的理论依 据， 用目测法来判定滴点终点。
方法中阴离子聚电解质聚乙烯硫酸钾 （Potassium Polyvinyl Sulfate, PVSK)作为滴定剂， 用带正电荷的蓝色有机染料甲苯胺蓝为 指示剂。 利用甲苯胺蓝 (Toluidine Blue, T. Β)为指示剂是因为它带正电 荷， 在酸性溶液中不与壳聚糖发生电荷中和反应， 但它可与带负电荷的 聚乙烯硫酸钾结合，不过，这种结合滞后于正、负聚电解质之间的反应。 在滴定终点前， 溶液为蓝色， 到达终点时， 过量的聚乙烯硫酸钾立即与 甲苯胺蓝反应， 从而使溶液由蓝色变成紫红色， 并生成絮状沉淀， 由于 颜色转变很灵敏， 所以可以准确判定滴定的终点。
基于上述理论及本发明的目的， 本发明所采用的技术方案是：
1、 一种用于测定纺织品中壳聚糖纤维含量的方法， 其特征在于， 包括以下步骤：
步骤一： 称取恒重且不大于 2g待测紡织品,将其放置于锥形瓶中， 加入 1%的醋酸溶液 100ml溶解待测纺织品中的壳聚糖纤维，待用；
在所述溶解过程中， 最好搅拌待测纺织品 4小时， 然后静置 0. 5小 时； 这样可以使待测纺织品中的壳聚糖纤维在醋酸溶液中充分溶解。
步骤二： 称取步骤一中待用溶液放入另一个锥形瓶中， 再加入去离 子水， 后加入甲苯胺蓝指示剂， 待用；
步骤三： 把磁力转子放入所述步骤二中盛有待用溶液的锥形瓶中， 再将所述锥形瓶放置在磁力搅拌器上；边搅动边将聚乙烯硫酸钾滴定液 滴入， 溶液由蓝色变为紫红色， 并在 20s内不褪色并出现絮状沉淀物， 即为滴定终点， 读取聚乙烯硫酸钾滴定液的滴定体积 vl;
在滴入聚乙烯硫酸钾滴定液过程中， 滴定速度最好控制在
0.03ml/s, 若滴定速度过快， 往往会出现暂时的滴定终点现象。
步骤四： 取与步骤二中相同数量的去离子水， 按上述同样的方法进 行空白滴定， 读取聚乙烯硫酸钾滴定液的滴定体积 V2;
步骤五： 按下式计算纺织品中壳聚糖纤维的含量：
W!=CXfX (V1-V2) X0.001X161.15X100/W3
W2=CXfX (V1-V2) X 0.001X203.19X100/ W3X (1-D · D) /D-D g=( Wx+ W2)/ WX100%
本发明的技术效果是：本发明的技术方案用公式的方式提供了一种 用于测定纺织品中壳聚糖纤维含量的方法， 误差很小， 建立一种具有高 灵敏度的简便、 快速测定纺织品中壳聚糖纤维含量的方法。
说明书中标号说明：
C ~~聚乙烯硫酸钾滴定液的物质量的浓度， 0.0025mol/L
f——聚乙烯硫酸钾滴定液的系数 1.01
W,——待测物中壳聚糖纤维脱去乙酰基的纤维重量， g
W2——待测物中壳聚糖纤维未脱去乙酰基的纤维重量， g
Vt——步骤三中聚乙烯硫酸钾滴定液的滴定量， ml,
V2——步骤四中聚乙烯硫酸钾滴定液的滴定量， ml，
W3——步骤二中称取的溶液的重量， 在本发明实施例中指 5g
W——待测物的克数， g
161.15——壳聚糖中一个糖单元的相对分子质量
203.19——甲壳素中一个糖单元的相对分子质量
D -D——壳聚糖（纤维）的脱乙酰度（deacetylation degree，縮写为 D -D) g ^"-纺织品中壳聚糖纤维的含量， ％ 具体实施方式
现对本发明提供的一种用于测定纺织品中壳聚糖纤维含量的方法进 行详细说明：
实施例一：
步骤一：用万分之一的电子天平称取恒重的含壳聚糖纤维待测纺织 品 lg， 其成分壳聚糖纤维 /纽代尔黏胶 /棉的比例是 10/30/60， 壳聚糖 纤维的脱乙酰度（deacetylation degree, 缩写为 D · D为 93%, 将其剪 碎放置于 100ml的锥形瓶中，然后加入 1%的醋酸溶液 100ml溶解待测纺 织品中的壳聚糖纤维；
作为本步骤的优化， 在所述溶解过程中， 最好搅拌待测纺织品 4小 时， 然后静置 0. 5小时， 这样可以使待测纺织品中的壳聚糖纤维在醋酸 溶液中充分溶解。
步骤二：用万分之一的电子天平准确称取步骤一中待用溶液 5g放入 150ml的锥形瓶中，再加入 50ml去离子水，后加入 2-3滴甲苯胺蓝指示 剂待用； 所述的甲苯胺蓝由上海国药集团试剂有限公司生产， 沪试牌， 甲苯胺蓝指示剂的浓度为 0. 1%, 其溶剂为去离子水。
步骤三： 把磁力转子放入所述步骤二中盛有待用溶液的锥形瓶中， 再将所述锥形瓶放置在磁力搅拌器上；边搅动边将聚乙烯硫酸钾滴定液 滴入， 溶液由蓝色变为紫红色， 并在 20s内不褪色并出现絮状沉淀物， 即为滴定终点， 读取聚乙烯硫酸钾滴定液的滴定体积 ^是 11. 23ml; 所述聚乙烯硫酸钾滴定液是由日本和光纯药工业株式会社生产，品 名： 聚乙烯硫酸钾滴定液， 代码 NO. 162- 21655;
作为本步骤的优化， 在滴入聚乙烯硫酸钾滴定液过程中， 滴定速度 最好控制在 0. 03ml/s，若滴定速度过快，往往会出现暂时的滴定终点现 象。
步骤四： 取 50ml去离子水， 按上述同样的方法进行空白滴定， 并 读取聚乙烯硫酸钾滴定液的滴定体积 V2是 0.15ml;
步骤五： 按下式计算纺织品中壳聚糖纤维的含量：
Wt=C X f X (Vi— V2) X 0.001 X 161.15 X 100/W3=0.0025 X 1.01 X (11.23ml— 0.15ml ) X0.001X161.15 X 100/5=9.02 X 10— 2
W2二 CXfX (V厂 V2) X 0.001X203.19X100/ W3X (1- D · D) /D · D =0.0025 X 1.01 X (11.23ml - 0.15ml) X 0.001 X 203.19 X 100/5 X (1-93%) /93%=8.56X10"3
g= ( Wi+ W2)/ WX 100%= (9.02 X 10 +8.56 X 10"3) /1 X 100%=9.88% 其误差为： 10%- 9.88%=0.12%。
实施例二：
步骤一： 用万分之一的电子天平称取恒重的含壳聚糖纤维待测纺织 品 0.6g， 其成分壳聚糖纤维 /棉的比例是 15/85, 壳聚糖纤维的脱乙酰 度（deacetylation degree,縮写为 D *D为 93%，将其剪碎放置于 100ml 的锥形瓶中，然后加入 1%的醋酸溶液 100ml溶解待测纺织品中的壳聚糖 纤维； .
最好搅拌待测纺织品 4小时， 然后静置 0.5小时， 这样更能充分溶 解待测纺织品中的壳聚糖纤维；
步骤二：用万分之一的电子天平准确称取步骤一中待用溶液 5g放入 150ml的锥形瓶中，再加入 50ml去离子水，后加入 2-3滴甲苯胺蓝指示 剂待用，所述的甲苯胺蓝由上海国药集团试剂有限公司生产， 沪试牌， 甲苯胺蓝指示剂的浓度为 0.1%, 其溶剂为去离子水。
步骤三： 把磁力转子放入所述步骤二中盛有待用溶液的锥形瓶中， 再将所述锥形瓶放置在磁力搅拌器上；边搅动边将聚乙烯硫酸钾滴定液 滴入，最好速度控制在 0.03ml/s，若滴定速度过快，往往会出现暂时的 滴定终点现象； 在滴定过程中， 溶液由蓝色变为紫红色， 若在 20s内不 褪色并出现絮状沉淀物， 即达到滴定终点， 读取聚乙烯硫酸钾滴定液的 滴定体积 ^是 10.30ml;
所述聚乙烯硫酸钾滴定液是由日本和光纯药工业株式会社生产，品 名： 聚乙烯硫酸钾滴定液， 代码 NO.162-21655;
步骤四： 取 50ml去离子水， 按上述同样的方法进行空白滴定， 并 读取聚乙烯硫酸钾滴定液的滴定体积 V2是 0.15ml;
步骤五： 按下式计算紡织品中壳聚糖纤维的含量：
W!=C X f X (V! - V2) X 0.001 X 161.15 X 100/W3=0.0025 X 1.01 X
(10.30ml— 0.15ml ) X0.001X161.15 X 100/5=8.26 X 10— 2
W2=CXfX (V厂 V2) X 0.001X203.19X100/ W3X (1-D · D) /D · D
=0.0025 X 1.01 X (10.30ml - 0.15ml ) X 0.001 X 203.19 X 100/5 X (1-93%) /93%=7.84X10—3
g=( Wi+ W2) /WX 100%=(8.26X 10— 2+7.84X 10— 3)/ 0.6X10096=15.07% 其误差为： 15.07%- 15%=0.07%，
以上检测实例产生误差的原因：其一，混纺织物中存在不匀率问题。 其二， 因人的视觉误差导致滴定终点存在差异。其三， 因人的视觉误差 导致读取仪器刻度时存在差异。

Claims

权 利 要 求 书
1、 一种用于测定纺织品中壳聚糖纤维含量的方法， 其特征在于， 包括以下步骤：
步骤一： 称取恒重且不大于 2g待测纺织品，将其放置于锥形瓶中， 加入 1%的醋酸溶液 100ml溶解待测纺织品中的壳聚糖纤维， 待用； 步骤二： 称取步骤一中待用溶液放入另一个锥形瓶中， 再加入去离 子水， 后加入甲苯胺蓝指示剂， 待用； 步骤三： 把磁力转子放入所述步骤二中盛有待用溶液的锥形瓶中， 再将所述锥形瓶放置在磁力搅拌器上；边搅动边将聚乙烯硫酸钾滴定液 滴入， 溶液由蓝色变为紫红色， 并在 20s内不褪色并出现絮状沉淀物， 即为滴定终点， 读取聚乙烯硫酸钾滴定液的滴定体积 VI； 步骤四： 取与步骤二中相同数量的去离子水， 按上述同样的方法进 行空白滴定， 读取聚乙烯硫酸钾滴定液的滴定体积 V2; 步骤五： 按下式计算纺织品中壳聚糖纤维的含量：
Wl=CX fX(Vl -V2)X0.001 X 161.15 X 100AV3
W2=C X fX (VI— V2) X 0.001 X 203.19 X 100/ W3 X ( 1-D'D) /D-D g=( Wl+ W2)/ Wxl00%
2、 如权利要求 1所述的一种用于测定纺织品中壳聚糖纤维含量 的方法， 其特征在于， 在所述步骤一中， 所述溶解过程中， 搅拌待测纺 织品 4小时， 然后静置 0.5小时。
3、如权利要求 1或 2所述的一种用于测定纺织品中壳聚糖纤维含 量的方法，其特征在于，在所述步骤三中，滴入聚乙烯硫酸钾滴定液时， 速度控制在 0.03ml/s。
PCT/CN2011/071932 2010-04-29 2011-03-24 一种用于测定纺织品中壳聚糖纤维含量的方法 WO2011134326A1 (zh)

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