WO2011086189A3 - Produits de recombinaison, vecteurs et cyanobactéries pour la synthèse d'alcools gras, et procédés de production d'alcools gras dans des cyanobactéries - Google Patents

Produits de recombinaison, vecteurs et cyanobactéries pour la synthèse d'alcools gras, et procédés de production d'alcools gras dans des cyanobactéries Download PDF

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Publication number
WO2011086189A3
WO2011086189A3 PCT/EP2011/050555 EP2011050555W WO2011086189A3 WO 2011086189 A3 WO2011086189 A3 WO 2011086189A3 EP 2011050555 W EP2011050555 W EP 2011050555W WO 2011086189 A3 WO2011086189 A3 WO 2011086189A3
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WO
WIPO (PCT)
Prior art keywords
cyanobacteria
fatty alcohols
synthesis
constructs
vectors
Prior art date
Application number
PCT/EP2011/050555
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English (en)
Other versions
WO2011086189A2 (fr
Inventor
Xuefeng Lu
Xiaoming Tan
Fengxia Qi
Quan LUO
Lun Yao
Qianqian Gao
Original Assignee
Shell Internationale Research Maatschappij B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN201010034404.4A external-priority patent/CN102127563B/zh
Priority claimed from CN201010213758.5A external-priority patent/CN102311966B/zh
Application filed by Shell Internationale Research Maatschappij B.V. filed Critical Shell Internationale Research Maatschappij B.V.
Priority to BR112012017456A priority Critical patent/BR112012017456A2/pt
Priority to EP11700428A priority patent/EP2524035A2/fr
Priority to CA2786244A priority patent/CA2786244A1/fr
Publication of WO2011086189A2 publication Critical patent/WO2011086189A2/fr
Publication of WO2011086189A3 publication Critical patent/WO2011086189A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Liquid Carbonaceous Fuels (AREA)

Abstract

La présente invention concerne des produits de recombinaison, des vecteurs et des cyanobactéries pour la synthèse d'alcools gras, et des procédés de production d'alcools gras dans des cyanobactéries. Spécifiquement, la présente invention concerne un produit de recombinaison pour la synthèse d'alcools gras dans des cyanobactéries, un vecteur comprenant le produit de recombinaison, une cyanobactérie comprenant le produit de recombinaison ou transformée par le vecteur, et un procédé de production d'alcools gras dans des cyanobactéries.
PCT/EP2011/050555 2010-01-15 2011-01-17 Produits de recombinaison, vecteurs et cyanobactéries pour la synthèse d'alcools gras, et procédés de production d'alcools gras dans des cyanobactéries WO2011086189A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
BR112012017456A BR112012017456A2 (pt) 2010-01-15 2011-01-17 "construto, vetor, cianobact´peria, método para produzir alcoóis graxos em uma cianobactéria, biocombustível, e, método de expressão de genes exógenos"
EP11700428A EP2524035A2 (fr) 2010-01-15 2011-01-17 Produits de recombinaison, vecteurs et cyanobactéries pour la synthèse d'alcools gras, et procédés de production d'alcools gras dans des cyanobactéries
CA2786244A CA2786244A1 (fr) 2010-01-15 2011-01-17 Produits de recombinaison, vecteurs et cyanobacteries pour la synthese d'alcools gras, et procedes de production d'alcools gras dans des cyanobacteries

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN201010034404.4 2010-01-15
CN201010034404.4A CN102127563B (zh) 2010-01-15 2010-01-15 一种集胞藻pcc6803表达外源基因的方法
CN201010213758.5A CN102311966B (zh) 2010-06-30 2010-06-30 用于合成脂肪醇的构建体，载体，蓝细菌，以及在蓝细菌中生产脂肪醇的方法
CN201010213758.5 2010-06-30

Publications (2)

Publication Number Publication Date
WO2011086189A2 WO2011086189A2 (fr) 2011-07-21
WO2011086189A3 true WO2011086189A3 (fr) 2011-10-13

Family

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PCT/EP2011/050555 WO2011086189A2 (fr) 2010-01-15 2011-01-17 Produits de recombinaison, vecteurs et cyanobactéries pour la synthèse d'alcools gras, et procédés de production d'alcools gras dans des cyanobactéries

Country Status (4)

Country Link
EP (1) EP2524035A2 (fr)
BR (1) BR112012017456A2 (fr)
CA (1) CA2786244A1 (fr)
WO (1) WO2011086189A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111235172B (zh) * 2020-02-24 2021-10-19 扬州大学 基于lacZ基因和pUC复制子的原核启动子报告系统及其构建方法和应用

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013029027A1 (fr) * 2011-08-24 2013-02-28 Algenol Biofuels Inc. Séparation de la biomasse productive de la biomasse non productive dans des bioréacteurs et procédés associés
CN102952818B (zh) 2011-08-26 2016-03-02 中国科学院青岛生物能源与过程研究所 用于在蓝细菌中提高脂肪醇产量的构建体和方法
CN109097378B (zh) * 2018-08-13 2021-08-03 中国科学院青岛生物能源与过程研究所 一种异戊二烯合酶和其编码基因、表达载体、工程菌以及生产异戊二烯的方法及应用
CN111041038B (zh) * 2019-12-02 2022-11-08 天津大学 高效生物合成虾青素的集胞藻6803基因工程菌及构建方法及应用
CN111549047A (zh) * 2020-04-20 2020-08-18 山东大学 驱动外源基因在蓝藻中高效表达的启动子的筛选方法
CN111560340B (zh) * 2020-04-21 2022-08-23 天津大学 一种生物合成肌醇的集胞藻基因工程菌及构建方法及应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143538A (en) * 1997-02-20 2000-11-07 The United States Of America As Represented By The United States Department Of Energy Fatty acyl-CoA reductase
WO2007136762A2 (fr) * 2006-05-19 2007-11-29 Ls9, Inc. Production d'acides gras et de leurs dérivés
WO2008119082A2 (fr) * 2007-03-28 2008-10-02 Ls9, Inc. Production améliorée de dérivés d'acides gras
WO2011019858A1 (fr) * 2009-08-11 2011-02-17 Synthetic Genomics, Inc. Production microbienne d'alcools gras

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143538A (en) * 1997-02-20 2000-11-07 The United States Of America As Represented By The United States Department Of Energy Fatty acyl-CoA reductase
WO2007136762A2 (fr) * 2006-05-19 2007-11-29 Ls9, Inc. Production d'acides gras et de leurs dérivés
WO2008119082A2 (fr) * 2007-03-28 2008-10-02 Ls9, Inc. Production améliorée de dérivés d'acides gras
WO2011019858A1 (fr) * 2009-08-11 2011-02-17 Synthetic Genomics, Inc. Production microbienne d'alcools gras

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CHEN Z ET AL: "Expression of mammalian metallothionein-I gene in cyanobacteria to enhance heavy metal resistance", MARINE POLLUTION BULLETIN, vol. 39, no. 1-12, January 1999 (1999-01-01), pages 155 - 158, XP002634176, ISSN: 0025-326X *
DOAN T T P ET AL: "Functional expression of five Arabidopsis fatty acyl-CoA reductase genes in Escherichia coli", JOURNAL OF PLANT PHYSIOLOGY, vol. 166, no. 8, 15 May 2009 (2009-05-15), pages 787 - 796, XP026056154, ISSN: 0176-1617, [retrieved on 20081204], DOI: DOI:10.1016/J.JPLPH.2008.10.003 *
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LEE DAE-WEON: "Mosquito larvicidal activity of Synechocystis PCC6803 transformed with the cry11Aa gene to Culex tritaeniorhynchus and Anopheles sinensis", KOREAN JOURNAL OF APPLIED ENTOMOLOGY, vol. 43, no. 1, 1 March 2004 (2004-03-01), pages 35 - 41, XP008135831, ISSN: 1225-0171 *
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111235172B (zh) * 2020-02-24 2021-10-19 扬州大学 基于lacZ基因和pUC复制子的原核启动子报告系统及其构建方法和应用

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Publication number Publication date
EP2524035A2 (fr) 2012-11-21
WO2011086189A2 (fr) 2011-07-21
BR112012017456A2 (pt) 2015-09-15
CA2786244A1 (fr) 2011-07-21

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