WO2011059170A2 - Procédé d'extraction d'une substance aromatique à partir de plantes médicinales chinoises traditionnelles - Google Patents

Procédé d'extraction d'une substance aromatique à partir de plantes médicinales chinoises traditionnelles Download PDF

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WO2011059170A2
WO2011059170A2 PCT/KR2010/006118 KR2010006118W WO2011059170A2 WO 2011059170 A2 WO2011059170 A2 WO 2011059170A2 KR 2010006118 W KR2010006118 W KR 2010006118W WO 2011059170 A2 WO2011059170 A2 WO 2011059170A2
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extraction tank
extracting
weight
mixture
supercritical
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PCT/KR2010/006118
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Korean (ko)
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WO2011059170A3 (fr
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임강현
김정범
김이화
송효남
안철현
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주식회사 자연인
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/34—Campanulaceae (Bellflower family)
    • A61K36/346—Platycodon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/234—Cnidium (snowparsley)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/284—Atractylodes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484—Glycyrrhiza (licorice)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/534—Mentha (mint)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/538—Schizonepeta
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
    • A61K36/708—Rheum (rhubarb)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents

Definitions

  • the present invention relates to a method of extracting the aromatic substance from the wind-permeable acid Chinese herbal medicine, and more particularly, to a method for extracting the aromatic substance from the wind-permeable acid herb such as licorice, gilyeong, rhubarb, peppermint, baekryeung, cheonggung, hyunggae.
  • the technique for extracting the wind-break through acid active ingredients shows the same effect even if the extraction of each of the natural product extract and then mixed with this method is equally applicable. That is, while extracting the solvent evaporated using a distillation apparatus equipped with a cooling condenser, the wind-permeable acid natural product mixed extract is concentrated under reduced pressure to obtain an extract.
  • the present invention has been made to solve the above problems, it is an object of the present invention to provide an oil refining method for Windproof Tongseong acid to reduce the extraction time while simplifying the process, unlike the existing oil refining method.
  • an object of the present invention is to provide a windproof acidic essential oil composition which can enhance the effects of appetite suppression and decay using the essential oil obtained through the windproof acidic oil refining method.
  • the present invention relates to a method for efficiently extracting aromatic components from wind-permeable herbal medicines.
  • the process is simple, and the extraction time can be reduced, the dietary fragrance composition prepared by the present invention provides a stable fragrance to the user, where the divergent fragrance is installed fragrance It provides a windproof acidic essential oil composition that can maintain the fragrance for a long time around.
  • the herbal medicine to health the human body as a constituent of the windproof Tongseong acid essential oil composition obtained through the extraction method, by providing the composition to help the health of the human body by the scent of the herbal medicine and further herbal natural fragrance Entering the human body has the effect of suppressing appetite, causing the effect of weight loss.
  • 1 is a flow chart illustrating a method for extracting the aromatic substance from the wind-permeable acid herbal medicine of the present invention.
  • Figure 2 is a graph showing the change in Leptin positive cell expression of the test subject group.
  • Figure 3 is a graph showing the change in NPY positive cell expression of the test subject group.
  • Figure 5 is a graph showing the change in BDNF protein expression of the test subject group.
  • the present invention comprises a first step of mixing and grinding licorice, gilyeong, rhubarb, peppermint, baekryeung, cheongung, and mold, and mixing and pulverizing the mixture obtained through the first step into a supercritical extraction tank and supplying a supercritical fluid.
  • Step 2 and adjusting the internal pressure of the supercritical extraction tank supplied with the mixture to 200 to 500 atm and adjusting the temperature to 30 to 50 ° C., and then maintaining the supercritical extraction tank for 5 to 7 hours.
  • 5 to 7 hours after elapse of the internal pressure of the supercritical extraction tank is characterized in that it comprises a fourth step of extracting the extract from the mixture stored therein.
  • the method of extracting the aromatic substance from the present inventors Bangpungtongseong Herbal Medicine is through the first step (S100) and the first step (S100) of mixing and drying the licorice, Gilyeong, rhubarb, peppermint, Baekchul, Cheonggyeong, and dried.
  • the second step (S200) of putting the pulverized mixture into a supercritical extraction tank and supplying a supercritical fluid adjusts the internal pressure of the supercritical extraction tank supplied with the mixture to 200 to 500 atm, and the temperature is 30 to 50 ° C.
  • the fourth step (S400) and the extract extracted through the fourth step (S400) is made of a fifth step (S500) to remove the wax components and non-volatiles by dissolving using a solvent.
  • the licorice, gilkyung, rhubarb, peppermint, baekchul, cheongung, and hyunggae that make up Bangfeng Tongseong medicinal herb are as follows.
  • the licorice not only sweetens bitter medicine to make it easier to eat, but also relieves the toxicity of all medicines, coughs and coughs, and neutralizes all medicines. Keeps the physiology of the nose, mouth and ear stool normal, communicates all blood veins, strengthens muscles and bones, improves nutrition, detoxifies the toxicity of all medicines, harmonizes 72 stones and 1200 herbs It is written to make the drug appear well. It is also effective in releasing tension and acting as an allergy to stomach ulcer and duodenal ulcer.
  • Gil-Kyong is a bellflower plant that enhances cellular immunity, enhances mucus content of mucous membranes, and promotes drainage.It is also effective in wound healing. It also helps to promote blood circulation and manages the skin.
  • rhubarb makes the human body very cold and has the effect of removing blood that is not clean for the body. It has been found that when the rhubarb is added to other herbal fragrance compositions, it has been developed to prevent the entire fragrance from diverging easily.
  • Mint also brightens the head and eyes, sweats, and has antipyretic effects on colds and headaches.
  • the scent is hot and slightly bitter, and makes the user feel refreshed.
  • the spicy and refreshing scent of the 'mint' stimulates the central nerve and indirectly propagates to the peripheral nerve, thereby facilitating the enlargement of skin capillaries and the secretion of sweat glands, thereby acting on the human body by sweating.
  • Baekchul has the effect of strengthening the rain, strengthening the rain, improving diarrhea and stopping sweat.
  • Baekchul is a chrysanthemum and shovel plant whose roots and stems are used as medicine. Its constituents include petroleum essential oils, the main ones of which are atractylon, atractylenolide I, II and III, and vitamin A substances.
  • hyunggae has the effect of clearing head and eyes and releasing cold headache and cold energy.It contains a large amount of volatile essential oils, and its scent is slightly spicy and similar to peppermint. And, nerve cramps are stabilized, the blood circulation of the skin is active.
  • the definition of the word supercritical fluid shows that all pure materials show the state of gas and liquid solids according to temperature and pressure changes.
  • the vapor pressure curve which is the phase transition curve of gas and liquid, shows that as the temperature increases, It can be seen that the pressure also increases. This results in an increase in vapor pressure as a result of reaching a new equilibrium point, which in turn reduces the difference in density between the liquid phase and the gas phase.
  • the density difference between the liquid and the gas is the same at the critical point and cannot be distinguished. In the region above the critical point, liquefaction does not occur even if the pressure is increased, and vaporization does not occur even when the temperature is increased.
  • a fluid having an intermediate nature between a gas and a liquid is defined as a supercritical fluid.
  • Supercritical fluids vary greatly in density, even with small changes in temperature and pressure, making it easy to control dissolving power and have inherent characteristics different from gases and liquids. In other words, it is characteristic of liquid in terms of dissolution related to the interaction between solvent and solute molecules, density, which is closely related to the ability to separate solute from matrix, and related to substrate permeability. High difficulty, low surface tension, etc., indicate gas properties.
  • Supercritical Fluid Extraction Technology is a technology that uses fluids above the critical temperature and pressure to replace existing processes in the extraction and refining fields of pharmaceuticals, food processing and petrochemical refining. It is attracting attention as an environmentally friendly technology. In particular, in advanced countries, gas and liquids have been used in traditional processes for the past 30 years due to the rise of energy resource prices, the environmental pollution of traditional separation processes, and the expansion of demand for special purpose new materials that cannot be manufactured by gas or liquid processes. We have been devoting our to the development of new process fluid technology that uses supercritical fluid technology as a process fluid.
  • Supercritical fluid extraction is a technology that takes advantage of the advantages of supercritical fluids and has a number of unique advantages because of the complex nature of distillation and extraction.
  • the supercritical fluid can be set to any condition from high density to low density by manipulating the pressure temperature, so it has excellent selectivity, such as fractionation and separation, so that a high purity product can be obtained and the extraction solvent can be recovered almost completely without loss. And a purified product with no residual solvent can be obtained.
  • the viscosity of the supercritical fluid is small, the permeability to the sample is good, the extraction efficiency is high, and the diffusion coefficient is large, so the extraction speed is high, and extraction at a relatively low temperature can avoid damage by heat, and the sample and the super Since the density difference with the critical fluid is large and the viscosity of the supercritical fluid is low, it has many advantages, such as the separation of the extraction residue and the solvent.
  • Supercritical fluid extraction method uses carbon dioxide as a supercritical fluid and the extraction process is as follows.
  • each effective ingredient is well expressed after each extraction and the synergistic effect of each other can be obtained.
  • the fragrance essential oil composition for diet which is a mixed essential oil extract of licorice, gilyeong, rhubarb, peppermint, baekchul, cheongung, hyunggae according to the present invention.
  • the mixing ratio of the mixture is a fragrance for diet, Experiments have confirmed this to be optimal for functioning.
  • the mixture is introduced into a supercritical extraction tank, and the pressure is increased by supplying carbon dioxide to the extraction tank using a high pressure gas pump.
  • the pressure of the extraction tank reaches 450 atm, the supply of carbon dioxide is stopped.
  • the temperature inside the extraction tank is maintained at 25 to 35 degrees Celsius. The reason for this is that a long pipe (1m) is connected between the pressure pump and the extraction tank so that the temperature close to the pressure pump is warmed, but the temperature inside the extraction tank does not change. There will be no.
  • the temperature inside the extraction tank is heated to 65 degrees Celsius at a rate of 0.5 ° C./min using a heating wire surrounding the outside of the extraction tank using a temperature controller.
  • the pressure of the extraction tank due to the elevated temperature discharges a part of carbon dioxide by using a pressure regulator attached to the outlet of the extraction tank and maintains the pressure at 300 atm.
  • the released extract was decompressed to 1 atmosphere to release carbon dioxide into the atmosphere, and the active ingredient of the aromatic essential oil extract was recovered together into the collector.
  • the above process is carried out for 12 hours to obtain an extract, and then the extraction operation is terminated.
  • fragrance inhalation essential oil composition fragrance inhalation was put in a closed plastic box (35 ⁇ 23 ⁇ 24 cm) in advance the fragrance essential oil composition one day before the start of the experiment so that the fragrance is sufficiently spread in the plastic box.
  • the memory capacity tester used a step-down avoidance task (NeuroLab, Korea). The animals were allowed to settle for 2 minutes on the first 7 ⁇ 25 cm platform (2.5 cm in height) and then measured. Records were measured when all four feet touched a 1 cm-thick stainless steel bar (42 ⁇ 25 cm) placed parallel to the rats on the platform. Rats descended from the platform were given an electric stimulation of 0.2 mA for 2 seconds, the mice were memorized, and the test was performed one hour later. Record measurements were taken with the same procedure, but without electrical stimulation. All four feet on the platform recorded up to 180 seconds.
  • mice were anesthetized by injecting Zoletile 50 ® (Vibac, Carros, France) at 10 mg / kg into the abdominal cavity of the experimental animal. Injected. Subsequently, 4% paraformaldehyde (PFA) fixed solution was perfused with 100 mM phosphate buffer (PB) for 10 minutes, and the brain was extracted. After 4 hours, 4% PFA solution was precipitated and fixed for 24 hours. Fixed brain tissue was precipitated in 30% sucrose solution for one week, and then a continuous cross section was prepared to a thickness of 40 ⁇ m using a freezing microtome (Leica, Nussloch, Germany).
  • Zoletile 50 ® Vibac, Carros, France
  • H 2 O 2 hydrogen peroxide
  • 50 mM PBS 1% for 30 minutes to inactivate endogenous peroxidase present in tissues, washed three times with 50 mM PBS, and then washed with 1% bovine serum.
  • bovine serum After 1 hour of reaction with albumin (BSA) and 10% goat serum, rabbit anti-leptin antibody (1: 500, DiaSorni, Stillwater, MN, USA), 0.05% BSA and 0.3% Triton X-100 The reaction was carried out for 12 hours at room temperature with the primary antibody solution.
  • BSA albumin
  • rabbit anti-leptin antibody 1: 500, DiaSorni, Stillwater, MN, USA
  • Triton X-100 The reaction was carried out for 12 hours at room temperature with the primary antibody solution.
  • the extracted proteins were electrophoresed with SDS-polyacrylamide gels and then transferred to nitrocellulose membrane (Schleicher & Schuell GmbH, Dassel, Germany). After blocking the transcribed membrane, it was reacted with mouse actin antibody (1: 1000; Santa Cruz Biotech, Santa Cru, CA, USA) and rabbit BDNF antibody (1: 1000; Santa Cruz Biotech). Horseradish peroxidase-conjugated rabbit anti-mouse antibody (1: 2000; Amersham Pharmacia Biotech GmbH, Freiburg, Germany) was used as a secondary antibody to actin, and anti-rabbit antibody (1: 4000; Santa Cruz biotech) Primary antibody was used. Western blotting was confirmed by the enhanced chemiluminescence (ECL) detection system (Amersham Pharmacia Biothech GmbH).
  • ECL enhanced chemiluminescence
  • the data obtained in this experiment was calculated using the mean ⁇ standard error mean (SEM) for each item using the SPSS version 11.0 statistical program. Group-specific differences in Leptin, NPY, Latency time, and BDNF expression were assessed by one-way analysis of variance (ANOVA). The significance level was set to P ⁇ .05.
  • the number of leptin-positive cells showing appetite suppression in the hypothalamus of each group was 137.23 ⁇ 10.12 in the control group (A), 72.17 ⁇ 5.22 in the fasting group (B), fragrance inhalation essential oil composition for fasting-diet group ( In C) 111 ⁇ 8.41.
  • the number of leptin-positive cells was significantly decreased compared with the control group ( P ⁇ .05), whereas the number of leptin-positive cells was significantly higher in the inhalation group of the perfume essential oil composition for fasting-diet than the fasting group. Increased ( P ⁇ . 05). Therefore, as a result of this experiment, the expression of leptin, which indicates appetite suppression, was decreased by fasting, and the appetite was increased. The expression of leptin was increased by the inhalation of the fragrance essential oil composition for diet. Can know.
  • the number of NPY positive cells showing appetite in the hypothalamus of each group is 94.7 ⁇ 4.12 in the control group (A), 116.25 ⁇ 1.90 in the fasting group (B), fragrance inhalation essential oil composition for fasting-diet In group (C), 106 ⁇ 3.15.
  • the fasting group showed a significant decrease in short-term memory capacity compared to the control group ( P ⁇ .05), whereas the inhalation group of fragrance essential oil composition for fasting-diet had a shorter-term memory capacity than the fasting group.
  • a significant increase was seen ( P ⁇ .05). Therefore, as a result of this experiment, the short-term memory capacity was reduced by fasting, and it was found that the inhalation of the fragrance essential oil composition for diet improves the short-term memory capacity reduced by fasting.
  • the expression of nerve growth factor BDNF protein in the hippocampus was quantified by Western blotting. Referring to FIG. 5, when the expression of BDNF in the control group (A) was 1.00, the fasting group (B) was 0.71 ⁇ 0.02, and the fragrance-refining composition for the fasting-diet fragrance inhalation group (B) was 0.85 ⁇ 0.02.
  • the fasting group showed a significant decrease in BDNF protein expression compared to the control group ( P ⁇ .05).
  • the fragrance inhalation group for the fragrance essential oil composition for fasting-diet significantly increased BDNF protein expression compared to the fasting group ( P ⁇ .05). Therefore, as a result of this experiment, the expression of BDNF protein, which is a nerve growth factor, was decreased by fasting, and the fasting inhibited the generation of nerves, and the inhalation of the perfume perfume oil composition for diet increased the expression of BDNF protein decreased by fasting. It can be seen that the inhalation of the fragrance essential oil composition for diet promotes nerve growth.
  • Licorice 90g, Gilgyeong 90g, rhubarb 68g, peppermint 54g, white ginseng 90g, Cheongung 54g, 54g dried, mixed and pulverized mixture 500g was put into a supercritical extraction tank and carbon dioxide was supplied to the extraction tank using a high pressure gas pump. When the pressure reached 450 atm, the supply of carbon dioxide was stopped. The temperature inside the extraction tank was heated up to 65 degrees Celsius at a rate of 0.5 ° C./min through a heating wire surrounding the outside of the extraction tank using a temperature controller. The temperature and pressure of the extraction tank were maintained at 65 degrees Celsius and 450 atmospheres for 6 hours while partially discharging carbon dioxide using a pressure regulator attached to the outlet of the extraction tank.
  • the high-pressure gas pump was restarted to continuously supply carbon dioxide to the extraction tank at a flow rate of 20 L / min.
  • the extract is discharged using the pressure regulator attached to the outlet, and the temperature and pressure of the extraction tank are maintained at 65 ° C. and 450 atm for 6 hours.
  • the high pressure gas pump is restarted and carbon dioxide is continuously supplied to the extraction tank at a flow rate of 20 L / min.
  • the extract is discharged using a pressure regulator attached to the outlet side and the temperature and pressure of the extraction tank are kept constant.
  • the released extract is decompressed to atmospheric pressure to release carbon dioxide into the atmosphere, and the active ingredients of the fragrance composition for a diet are recovered together into the collector.
  • the above process was carried out for 12 hours to obtain an extract, and then the extraction operation was terminated to obtain 51.3 g of natural essential oil.
  • This essential oil was dissolved in 30 g of natural essential oil in 70 g of ethanol to remove wax components and non-volatile substances to prevent wind-breaking acid diet. 89 g of essential oil extract was obtained.
  • Table 1 1 time Episode 2 3rd time 4 times 5 times Average 89 g 92 g 90 g 91 g 93 g 91 g
  • This extract was obtained by extracting the essential oil extract of Fung Fung Tong acid by the general extraction method of 65 g using a rotary volatilizer.
  • the process is simple, and the extraction time can be reduced, the dietary fragrance composition prepared by the present invention provides a stable fragrance to the user, where the divergent fragrance is installed fragrance It provides a windproof acidic essential oil composition that can maintain the fragrance for a long time around.
  • the herbal medicine to health the human body as a constituent of the windproof Tongseong acid essential oil composition obtained through the extraction method, by providing the composition to help the health of the human body by the scent of the herbal medicine and further herbal natural fragrance Entering the human body has the effect of suppressing appetite, causing the effect of weight loss.

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Abstract

La présente invention concerne un procédé d'extraction d'une substance aromatique à partir de plantes médicinales chinoises traditionnelles, ce procédé comprenant une première étape consistant à sécher respectivement, puis à mixer et broyer les plantes suivantes : réglisse, platycodon à grandes fleurs, rhubarbe, menthe, atractylodis rhizoma alba, cnidium officinale et schizonepeta tenuifolia briq.; une deuxième étape consistant à placer le mélange dans une cuve d'extraction supercritique dans laquelle un fluide supercritique est acheminé; une troisième étape consistant à augmenter la pression et la température à l'intérieur de la cuve d'extraction dans des plages comprises respectivement entre 200 et 500 atm et entre 30 et 50℃, puis à maintenir la cuve d'extraction supercritique dans un état stationnaire pendant 5 à 7 heures; ainsi qu'une quatrième étape consistant à réduire la pression interne de la cuve d'extraction supercritique après 5 à 7 heures de manière à obtenir un extrait du mélange conservé dans la cuve. Comparativement aux procédés classiques d'extraction d'huile essentielle, le procédé selon l'invention est simple et présente un temps d'extraction réduit, et une composition préparée selon l'invention présente un arôme stable pour les utilisateurs. De tels arômes d'origine végétale offrent une composition bénéfique pour la santé et, lorsqu'ils sont inhalés, réduisent l'appétit et produisent ainsi un effet de perte de poids.
PCT/KR2010/006118 2009-11-12 2010-09-09 Procédé d'extraction d'une substance aromatique à partir de plantes médicinales chinoises traditionnelles WO2011059170A2 (fr)

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KR10-2009-0109209 2009-11-12
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KR1020100083182A KR101013940B1 (ko) 2009-11-12 2010-08-26 방풍통성산 한약재로부터 방향성 물질을 추출하는 방법

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KR101661120B1 (ko) * 2013-07-30 2016-09-30 (주)힐링네이처농업회사법인 신이 정유를 유효성분으로 하는 각성 효과 또는 집중력 향상용 향료 조성물
CN103497823B (zh) * 2013-10-19 2015-07-08 长沙昊瑞生物科技有限公司 一种带壳火麻籽的加工利用技术
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KR20210120733A (ko) 2020-03-27 2021-10-07 한국한의약진흥원 방풍통성산 추출물을 유효성분으로 포함하는 제２형 당뇨 또는 이상지질혈증의 예방, 개선 또는 치료용 조성물
KR20210120734A (ko) 2020-03-27 2021-10-07 한국한의약진흥원 방풍통성산 추출물을 유효성분으로 포함하는 당뇨병성 신장병증의 예방, 개선 또는 치료용 조성물

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