WO2011059170A2

WO2011059170A2 - Method for extracting aromatic substance from traditional chinese herb medicines

Info

Publication number: WO2011059170A2
Application number: PCT/KR2010/006118
Authority: WO
Inventors: 임강현; 김정범; 김이화; 송효남; 안철현
Original assignee: 주식회사 자연인
Priority date: 2009-11-12
Filing date: 2010-09-09
Publication date: 2011-05-19
Also published as: WO2011059170A3; KR101013940B1

Abstract

The present invention relates to a method for extracting aromatic substance from traditional Chinese herb medicines, the method comprising: a first step, in which licorice, platycodon grandiflorum, rheum genus, mint, atractylodis rhizome alba, cnidium officinale and schizonepeta tenuifolia briquette are dried respectively, and then mixed and crushed; a second step, in which the mixture is placed in a supercritical extraction tank and a supercritical fluid is fed thereto; a third step, in which the pressure and temperature inside the extraction tank are increased to a range of 200 to 500 atm and a range of 30 to 50℃, respectively, and then the supercritical extraction tank is maintained in a steady state for 5-7 hours; and a fourth step, in which the internal pressure of the supercritical extraction tank is reduced after 5-7 hours and an extract is obtained from the mixture kept in the tank. As compared with conventional methods for extracting essential oil, the present invention method features a simple process and shorter extraction time, and a composition prepared according to the present invention presents a stable aroma to users. Such herbal aromas offer a composition beneficial to human health, and once the herbal aromas are inhaled into the body they suppress appetite, thereby generating a weight-loss effect.

Description

Method of Extracting Aromatic Substances from Bangpungtongseong Herbal Herbs

The present invention relates to a method of extracting the aromatic substance from the wind-permeable acid Chinese herbal medicine, and more particularly, to a method for extracting the aromatic substance from the wind-permeable acid herb such as licorice, gilyeong, rhubarb, peppermint, baekryeung, cheonggung, hyunggae.

In the context of increasing interest in the environment throughout the industry, the process of extracting and purifying natural active ingredients, or extracting and purifying using existing organic solvents has problems such as environmental and human toxicity, non-selectivity for target components, and high cost. Recently, various attempts to introduce new processes have been sought at home and abroad.

In other words, as the technology that uses supercritical fluid as a process fluid rapidly spreads to various industries such as fine chemicals, energy, environment, and new materials, various traditional separation technologies are being replaced by new technologies using supercritical fluid processes.

As an example, there is a technique for extracting the windproof acid active ingredients Looking at this, the technology for extracting the windproof acid active ingredients distillation equipped with a cooling condenser to prevent the solvent from evaporating after the mixed extract in a solvent such as ethanol Extraction by heating at 50 ~ 95 ℃, 3 to 20 hours in the apparatus or by dipping at 5 ~ 37 ℃ for 1 to 15 days to extract the active ingredient and then evaporated to concentrate the solvent such as ethanol to extract essential oils.

At this time, the technique for extracting the wind-break through acid active ingredients shows the same effect even if the extraction of each of the natural product extract and then mixed with this method is equally applicable. That is, while extracting the solvent evaporated using a distillation apparatus equipped with a cooling condenser, the wind-permeable acid natural product mixed extract is concentrated under reduced pressure to obtain an extract.

However, since the extraction method is heated to a high temperature or using an organic solvent, due to evaporation, pyrolysis, oxidation, there is a problem that the natural active ingredients are destroyed and essential oils exhibiting efficacy are lost.

The present invention has been made to solve the above problems, it is an object of the present invention to provide an oil refining method for Windproof Tongseong acid to reduce the extraction time while simplifying the process, unlike the existing oil refining method.

In addition, an object of the present invention is to provide a windproof acidic essential oil composition which can enhance the effects of appetite suppression and decay using the essential oil obtained through the windproof acidic oil refining method.

In order to achieve the above object, licorice, gilyeong, rhubarb, mint, baekryeung, cheongunggi, the first step of drying and grinding after mixing, and the mixture pulverized through the first step into a supercritical extraction tank and the supercritical fluid In the second step of supplying and adjusting the internal pressure of the supercritical extraction tank supplied with the mixture to 200 ~ 500 atm and the temperature is adjusted to 30 ~ 50 ℃ and maintain the constant supercritical extraction tank for 5-7 hours And a fourth step of reducing the internal pressure of the supercritical extraction tank after 5-7 hours and extracting the extract from the mixture stored therein.

As described above, the present invention relates to a method for efficiently extracting aromatic components from wind-permeable herbal medicines.

According to the present invention, unlike the conventional essential oil extraction method, the process is simple, and the extraction time can be reduced, the dietary fragrance composition prepared by the present invention provides a stable fragrance to the user, where the divergent fragrance is installed fragrance It provides a windproof acidic essential oil composition that can maintain the fragrance for a long time around.

In addition, by using the herbal medicine to health the human body as a constituent of the windproof Tongseong acid essential oil composition obtained through the extraction method, by providing the composition to help the health of the human body by the scent of the herbal medicine and further herbal natural fragrance Entering the human body has the effect of suppressing appetite, causing the effect of weight loss.

1 is a flow chart illustrating a method for extracting the aromatic substance from the wind-permeable acid herbal medicine of the present invention.

Figure 2 is a graph showing the change in Leptin positive cell expression of the test subject group.

Figure 3 is a graph showing the change in NPY positive cell expression of the test subject group.

4 is a graph showing a change in short-term memory capacity of the test subject group.

Figure 5 is a graph showing the change in BDNF protein expression of the test subject group.

The present invention comprises a first step of mixing and grinding licorice, gilyeong, rhubarb, peppermint, baekryeung, cheongung, and mold, and mixing and pulverizing the mixture obtained through the first step into a supercritical extraction tank and supplying a supercritical fluid. Step 2, and adjusting the internal pressure of the supercritical extraction tank supplied with the mixture to 200 to 500 atm and adjusting the temperature to 30 to 50 ° C., and then maintaining the supercritical extraction tank for 5 to 7 hours. And, 5 to 7 hours after elapse of the internal pressure of the supercritical extraction tank is characterized in that it comprises a fourth step of extracting the extract from the mixture stored therein.

Hereinafter, a method of extracting an aromatic substance from wind-permeable acidic herbal medicine according to the present invention will be described with reference to the accompanying drawings.

The method of extracting the aromatic substance from the present inventors Bangpungtongseong Herbal Medicine is through the first step (S100) and the first step (S100) of mixing and drying the licorice, Gilyeong, rhubarb, peppermint, Baekchul, Cheonggyeong, and dried. The second step (S200) of putting the pulverized mixture into a supercritical extraction tank and supplying a supercritical fluid, adjusts the internal pressure of the supercritical extraction tank supplied with the mixture to 200 to 500 atm, and the temperature is 30 to 50 ° C. After adjusting, the third step (S300) of maintaining the supercritical extraction tank constant for 5 to 7 hours, and after 5-7 hours, the internal pressure of the supercritical extraction tank is reduced and extracts are extracted from the mixture stored therein. The fourth step (S400) and the extract extracted through the fourth step (S400) is made of a fifth step (S500) to remove the wax components and non-volatiles by dissolving using a solvent.

First of all, the licorice, gilkyung, rhubarb, peppermint, baekchul, cheongung, and hyunggae that make up Bangfeng Tongseong medicinal herb are as follows.

The licorice not only sweetens bitter medicine to make it easier to eat, but also relieves the toxicity of all medicines, coughs and coughs, and neutralizes all medicines. Keeps the physiology of the nose, mouth and ear stool normal, communicates all blood veins, strengthens muscles and bones, improves nutrition, detoxifies the toxicity of all medicines, harmonizes 72 stones and 1200 herbs It is written to make the drug appear well. It is also effective in releasing tension and acting as an allergy to stomach ulcer and duodenal ulcer.

In addition, Gil-Kyong is a bellflower plant that enhances cellular immunity, enhances mucus content of mucous membranes, and promotes drainage.It is also effective in wound healing. It also helps to promote blood circulation and manages the skin.

Next, rhubarb makes the human body very cold and has the effect of removing blood that is not clean for the body. It has been found that when the rhubarb is added to other herbal fragrance compositions, it has been developed to prevent the entire fragrance from diverging easily.

Mint also brightens the head and eyes, sweats, and has antipyretic effects on colds and headaches. The scent is hot and slightly bitter, and makes the user feel refreshed. The spicy and refreshing scent of the 'mint' stimulates the central nerve and indirectly propagates to the peripheral nerve, thereby facilitating the enlargement of skin capillaries and the secretion of sweat glands, thereby acting on the human body by sweating.

Baekchul has the effect of strengthening the rain, strengthening the rain, improving diarrhea and stopping sweat. Baekchul is a chrysanthemum and shovel plant whose roots and stems are used as medicine. Its constituents include petroleum essential oils, the main ones of which are atractylon, atractylenolide I, II and III, and vitamin A substances.

Next, it is known to have the effect of warming the human body, removing headaches, and passing through the throat, and releasing congestion by clearing blood, and the fragrance of the arch is slightly spicy and bitter. .

Lastly, hyunggae has the effect of clearing head and eyes and releasing cold headache and cold energy.It contains a large amount of volatile essential oils, and its scent is slightly spicy and similar to peppermint. And, nerve cramps are stabilized, the blood circulation of the skin is active.

Hereinafter, a supercritical fluid extraction method will be described as an extraction method.

First of all, the definition of the word supercritical fluid shows that all pure materials show the state of gas and liquid solids according to temperature and pressure changes. Among them, the vapor pressure curve, which is the phase transition curve of gas and liquid, shows that as the temperature increases, It can be seen that the pressure also increases. This results in an increase in vapor pressure as a result of reaching a new equilibrium point, which in turn reduces the difference in density between the liquid phase and the gas phase. The density difference between the liquid and the gas is the same at the critical point and cannot be distinguished. In the region above the critical point, liquefaction does not occur even if the pressure is increased, and vaporization does not occur even when the temperature is increased.

Above this critical point, a fluid having an intermediate nature between a gas and a liquid is defined as a supercritical fluid. Supercritical fluids vary greatly in density, even with small changes in temperature and pressure, making it easy to control dissolving power and have inherent characteristics different from gases and liquids. In other words, it is characteristic of liquid in terms of dissolution related to the interaction between solvent and solute molecules, density, which is closely related to the ability to separate solute from matrix, and related to substrate permeability. High difficulty, low surface tension, etc., indicate gas properties.

Supercritical Fluid Extraction Technology is a technology that uses fluids above the critical temperature and pressure to replace existing processes in the extraction and refining fields of pharmaceuticals, food processing and petrochemical refining. It is attracting attention as an environmentally friendly technology. In particular, in advanced countries, gas and liquids have been used in traditional processes for the past 30 years due to the rise of energy resource prices, the environmental pollution of traditional separation processes, and the expansion of demand for special purpose new materials that cannot be manufactured by gas or liquid processes. We have been devoting ourselves to the development of new process fluid technology that uses supercritical fluid technology as a process fluid. Supercritical fluid extraction is a technology that takes advantage of the advantages of supercritical fluids and has a number of unique advantages because of the complex nature of distillation and extraction. The supercritical fluid can be set to any condition from high density to low density by manipulating the pressure temperature, so it has excellent selectivity, such as fractionation and separation, so that a high purity product can be obtained and the extraction solvent can be recovered almost completely without loss. And a purified product with no residual solvent can be obtained.

In addition, since the viscosity of the supercritical fluid is small, the permeability to the sample is good, the extraction efficiency is high, and the diffusion coefficient is large, so the extraction speed is high, and extraction at a relatively low temperature can avoid damage by heat, and the sample and the super Since the density difference with the critical fluid is large and the viscosity of the supercritical fluid is low, it has many advantages, such as the separation of the extraction residue and the solvent.

Supercritical fluid extraction method according to the present invention uses carbon dioxide as a supercritical fluid and the extraction process is as follows.

1) drying, mixing and pulverizing licorice, gilyeong, rhubarb, peppermint, baekchul, cheonggung and mold dog respectively;

2) feeding the mixture into a supercritical extraction tank and supplying a supercritical fluid;

3) maintaining the pressure of the extraction tank at 450 atm and raising the temperature inside the extraction tank to 65 ° C. at a rate of 0.5 ° C./min;

4) maintaining the temperature and pressure of the extraction tank at 30 ~ 50 ℃, 200 ~ 500 at 5 to 7 hours to obtain a perfume composition for diet by reducing the pressure to atmospheric pressure;

5) dissolving in a solvent such as ethanol to remove the wax component and non-volatile substances to obtain a dietary fragrance composition.

Through the extraction in the supercritical conditions as described above, as well as the effective obtaining of the active ingredient, each effective ingredient is well expressed after each extraction and the synergistic effect of each other can be obtained.

Looking at the extraction method of the fragrance essential oil composition for diet, which is a mixed essential oil extract of licorice, gilyeong, rhubarb, peppermint, baekchul, cheongung, hyunggae according to the present invention.

First, each licorice, Gilyeong, rhubarb, peppermint, Baekchul, Cheongung, mold dry well, with respect to 90 parts by weight of licorice 80-100 parts by weight, rhubarb 60-80 parts by weight, mint 40-60 parts by weight, Baekchul 80 ~ Mix 100 parts by weight, 40 to 60 parts by weight, 40 to 60 parts by weight, 40 to 60 parts by weight, and finely grind the mixture using a grinder. The mixing ratio of the mixture is a fragrance for diet, Experiments have confirmed this to be optimal for functioning.

Next, the mixture is introduced into a supercritical extraction tank, and the pressure is increased by supplying carbon dioxide to the extraction tank using a high pressure gas pump. When the pressure of the extraction tank reaches 450 atm, the supply of carbon dioxide is stopped. At this time, the temperature inside the extraction tank is maintained at 25 to 35 degrees Celsius. The reason for this is that a long pipe (1m) is connected between the pressure pump and the extraction tank so that the temperature close to the pressure pump is warmed, but the temperature inside the extraction tank does not change. There will be no.

Thereafter, the temperature inside the extraction tank is heated to 65 degrees Celsius at a rate of 0.5 ° C./min using a heating wire surrounding the outside of the extraction tank using a temperature controller. The pressure of the extraction tank due to the elevated temperature discharges a part of carbon dioxide by using a pressure regulator attached to the outlet of the extraction tank and maintains the pressure at 300 atm.

Next, maintain the temperature and pressure of the extraction tank for 6 hours at 65 degrees Celsius, 450 atm. The high pressure gas pump is restarted and carbon dioxide is continuously supplied to the extraction tank at a flow rate of 20 L / min. At the same time, the extract is discharged using a pressure regulator attached to the outlet side and the temperature and pressure of the extraction tank are kept constant.

The released extract was decompressed to 1 atmosphere to release carbon dioxide into the atmosphere, and the active ingredient of the aromatic essential oil extract was recovered together into the collector. The above process is carried out for 12 hours to obtain an extract, and then the extraction operation is terminated.

In the following, the function of the fragrance essential oil composition for diet extracted according to the present invention will be described through an experimental example.

Experimental Example

1. Experimental subject

The animals used in this experiment were supplied with 5 weeks old (25 g ± 2) ICR strains from an official animal handling company (Orient Bio Co, Kyung-Gi, Korea). After three days of environmental adaptation, the animals were divided into three groups by random sampling, and the inhalation group for control oil (n = 8), fasting group (n = 8), and fragrance essential oil composition for fasting-diet (n = 8). The fasting rats were watered for 48 hours but not fed. Rats in each group were placed in a breeding case (30 cm × 20 cm) and kept in the same laboratory environment, with day and night alternated at 12 h intervals, with constant temperature (20 ± 2 ° C) and constant humidity (60%).

The fragrance inhalation essential oil composition fragrance inhalation was put in a closed plastic box (35 × 23 × 24 cm) in advance the fragrance essential oil composition one day before the start of the experiment so that the fragrance is sufficiently spread in the plastic box.

The inhalation of incense was performed twice a day at 10 am and 6 pm for 1 hour, and the essential oil composition fragrance inhalation group rats were repeatedly inhaled freely for 2 days.

2. Memory capacity measurement

The memory capacity tester used a step-down avoidance task (NeuroLab, Korea). The animals were allowed to settle for 2 minutes on the first 7 × 25 cm platform (2.5 cm in height) and then measured. Records were measured when all four feet touched a 1 cm-thick stainless steel bar (42 × 25 cm) placed parallel to the rats on the platform. Rats descended from the platform were given an electric stimulation of 0.2 mA for 2 seconds, the mice were memorized, and the test was performed one hour later. Record measurements were taken with the same procedure, but without electrical stimulation. All four feet on the platform recorded up to 180 seconds.

3. Organizational Treatment

After the experiment, animals were anesthetized by injecting Zoletile 50 ^® (Vibac, Carros, France) at 10 mg / kg into the abdominal cavity of the experimental animal. Injected. Subsequently, 4% paraformaldehyde (PFA) fixed solution was perfused with 100 mM phosphate buffer (PB) for 10 minutes, and the brain was extracted. After 4 hours, 4% PFA solution was precipitated and fixed for 24 hours. Fixed brain tissue was precipitated in 30% sucrose solution for one week, and then a continuous cross section was prepared to a thickness of 40 μm using a freezing microtome (Leica, Nussloch, Germany).

4. Immunohistochemistry

1) Leptin immunohistochemistry

Brain sections were selected and first reacted with hydrogen peroxide (H ₂ O ₂ ) diluted with 50 mM PBS 1% for 30 minutes to inactivate endogenous peroxidase present in tissues, washed three times with 50 mM PBS, and then washed with 1% bovine serum. After 1 hour of reaction with albumin (BSA) and 10% goat serum, rabbit anti-leptin antibody (1: 500, DiaSorni, Stillwater, MN, USA), 0.05% BSA and 0.3% Triton X-100 The reaction was carried out for 12 hours at room temperature with the primary antibody solution. After 1 hour of reaction with biotinylated rabbit secondary antibody (1: 2000, Vector Laboratories, Burlingame, CA, USA), the reaction was performed at room temperature for 1 hour with avidin-biotin-peroxidase complex (Vectastain ABC ^® kit, Vector Laboratories). . As a colorant, 3,3'-diaminobenzidinetetrahydrochloride (DAB) was diluted to 0.02% in 0.05M Tris-HCl (pH 7.6), and 0.03% H ₂ O ₂ was added for 5 minutes. The colored tissues were placed on gelatincoated slide glass, dried at room temperature for 2 hours, dehydrated with increasing ethanol concentrations of 70%, 80%, 90%, and 100%, and then transparentized with xylene and encapsulated with polymount.

2) NPY immunohistochemistry

Brain sections were selected and first reacted with hydrogen peroxide (H ₂ O ₂ ) diluted with 50% PBS 1% for 30 minutes to inactivate endogenous peroxidase present in tissues, washed three times with 50mM PBS, and then washed with 1% bovine serum. After 1 hour of reaction with albumin (BSA) and 10% goat serum, rabbit anti-NPY antibody (1: 500, DiaSorni, Stillwater, MN, USA), 0.05% BSA and 0.3% Triton X-100 The reaction was carried out for 12 hours at room temperature with the primary antibody solution. After 1 hour of reaction with biotinylated rabbit secondary antibody (1: 2000, Vector Laboratories), the reaction was performed at room temperature for 1 hour in avidin-biotin-peroxidase complex (VectastainABC®kit, Vector Laboratories). As a colorant, 3,3'-diaminobenzidine tetrahydrochloride (DAB) was diluted to 0.02% in 0.05M Tris-HCl (pH 7.6), and 0.03% H ₂ O ₂ was added for 5 minutes. The colored tissues were placed on gelatin-coated slide glass, dried at room temperature for 2 hours, dehydrated with increasing ethanol concentrations of 70%, 80%, 90%, and 100%, and then transparentized with xylene and encapsulated with polymount.

5. Measurement of BDNF Protein Expression

To analyze the expression of BDNF protein in the hippocampus, tissues were washed with PBS, and then 50 mM HEPES (pH 7.5), 150 mM NaCl, 10% glycerol, 1% Triton X-100, 1.5 mM magnesium chloride hexahydrate, 1 mM ethleneglycol-bis- ( lysis buffer containing β-aminoethy1 ether) -N, N'-tetraacetic acid (EGTA), 1 mM phenylmethylsulfonyl fluoride (PMSF), 2 μg / ml leupeptin, 1 μg / ml pepstatin, 1 mM sodium ortho vanadate and 100 mM sodium floride After the extraction, the protein was extracted, centrifuged and the supernatant was taken to quantify the protein. The extracted proteins were electrophoresed with SDS-polyacrylamide gels and then transferred to nitrocellulose membrane (Schleicher & Schuell GmbH, Dassel, Germany). After blocking the transcribed membrane, it was reacted with mouse actin antibody (1: 1000; Santa Cruz Biotech, Santa Cru, CA, USA) and rabbit BDNF antibody (1: 1000; Santa Cruz Biotech). Horseradish peroxidase-conjugated rabbit anti-mouse antibody (1: 2000; Amersham Pharmacia Biotech GmbH, Freiburg, Germany) was used as a secondary antibody to actin, and anti-rabbit antibody (1: 4000; Santa Cruz biotech) Primary antibody was used. Western blotting was confirmed by the enhanced chemiluminescence (ECL) detection system (Amersham Pharmacia Biothech GmbH).

6 . Data processing method

The data obtained in this experiment was calculated using the mean ± standard error mean (SEM) for each item using the SPSS version 11.0 statistical program. Group-specific differences in Leptin, NPY, Latency time, and BDNF expression were assessed by one-way analysis of variance (ANOVA). The significance level was set to P <.05.

7. Experimental Results

1) Changes in Leptin Positive Cell Expression

The number of leptin-positive cells showing appetite suppression in the hypothalamus of each group was 137.23 ± 10.12 in the control group (A), 72.17 ± 5.22 in the fasting group (B), fragrance inhalation essential oil composition for fasting-diet group ( In C) 111 ± 8.41.

In the fasting group, the number of leptin-positive cells was significantly decreased compared with the control group ( P <.05), whereas the number of leptin-positive cells was significantly higher in the inhalation group of the perfume essential oil composition for fasting-diet than the fasting group. Increased ( P <. 05). Therefore, as a result of this experiment, the expression of leptin, which indicates appetite suppression, was decreased by fasting, and the appetite was increased. The expression of leptin was increased by the inhalation of the fragrance essential oil composition for diet. Could know.

2) Change of NPY positive cell expression

Referring to Figure 3, the number of NPY positive cells showing appetite in the hypothalamus of each group is 94.7 ± 4.12 in the control group (A), 116.25 ± 1.90 in the fasting group (B), fragrance inhalation essential oil composition for fasting-diet In group (C), 106 ± 3.15.

In the fasting group, the number of NPY-positive cells was significantly increased compared to the control group ( P <.05). Decreases ( P <.05). Therefore, as a result of this experiment, NPY expression indicating appetite was increased by fasting, and the appetite was increased. NPY expression was decreased by inhalation of the fragrance essential oil composition for diet, and the inhalation decreased the appetite increased by fasting. Able to know.

3) Effect of inhalation of fragrance essential oil composition for diet on short term memory ability

In this experiment, a passive avoidance test was performed to evaluate the effect of inhalation of fragrance essential oil composition on diet on short-term memory. As shown in FIG. 4, the latency time was 84.0 ± 8.4 in the control group (A), 32.0 ± 4.2 in the fasting group (B), and 60.9 ± 3.2 in the fragrance inhalation group (C) for the fragrance essential oil composition for fasting-diet.

As a result, the fasting group showed a significant decrease in short-term memory capacity compared to the control group ( P <.05), whereas the inhalation group of fragrance essential oil composition for fasting-diet had a shorter-term memory capacity than the fasting group. A significant increase was seen ( P <.05). Therefore, as a result of this experiment, the short-term memory capacity was reduced by fasting, and it was found that the inhalation of the fragrance essential oil composition for diet improves the short-term memory capacity reduced by fasting.

4) Changes in BDNF Protein Expression

The expression of nerve growth factor BDNF protein in the hippocampus was quantified by Western blotting. Referring to FIG. 5, when the expression of BDNF in the control group (A) was 1.00, the fasting group (B) was 0.71 ± 0.02, and the fragrance-refining composition for the fasting-diet fragrance inhalation group (B) was 0.85 ± 0.02.

As a result, the fasting group showed a significant decrease in BDNF protein expression compared to the control group ( P <.05). On the other hand, the fragrance inhalation group for the fragrance essential oil composition for fasting-diet significantly increased BDNF protein expression compared to the fasting group ( P <.05). Therefore, as a result of this experiment, the expression of BDNF protein, which is a nerve growth factor, was decreased by fasting, and the fasting inhibited the generation of nerves, and the inhalation of the perfume perfume oil composition for diet increased the expression of BDNF protein decreased by fasting. It can be seen that the inhalation of the fragrance essential oil composition for diet promotes nerve growth.

Hereinafter, the effects of the present invention will be described in more detail through Examples and Comparative Examples.

[Example] Preparation of Wind-proof Tongshan Diet Essential Oil Extract

Licorice 90g, Gilgyeong 90g, rhubarb 68g, peppermint 54g, white ginseng 90g, Cheongung 54g, 54g dried, mixed and pulverized mixture 500g was put into a supercritical extraction tank and carbon dioxide was supplied to the extraction tank using a high pressure gas pump. When the pressure reached 450 atm, the supply of carbon dioxide was stopped. The temperature inside the extraction tank was heated up to 65 degrees Celsius at a rate of 0.5 ° C./min through a heating wire surrounding the outside of the extraction tank using a temperature controller. The temperature and pressure of the extraction tank were maintained at 65 degrees Celsius and 450 atmospheres for 6 hours while partially discharging carbon dioxide using a pressure regulator attached to the outlet of the extraction tank. Then, the high-pressure gas pump was restarted to continuously supply carbon dioxide to the extraction tank at a flow rate of 20 L / min. At the same time, the extract is discharged using the pressure regulator attached to the outlet, and the temperature and pressure of the extraction tank are maintained at 65 ° C. and 450 atm for 6 hours. The high pressure gas pump is restarted and carbon dioxide is continuously supplied to the extraction tank at a flow rate of 20 L / min. At the same time, the extract is discharged using a pressure regulator attached to the outlet side and the temperature and pressure of the extraction tank are kept constant.

The released extract is decompressed to atmospheric pressure to release carbon dioxide into the atmosphere, and the active ingredients of the fragrance composition for a diet are recovered together into the collector. The above process was carried out for 12 hours to obtain an extract, and then the extraction operation was terminated to obtain 51.3 g of natural essential oil. This essential oil was dissolved in 30 g of natural essential oil in 70 g of ethanol to remove wax components and non-volatile substances to prevent wind-breaking acid diet. 89 g of essential oil extract was obtained.

Table 1

1 time	Episode 2	3rd time	4 times	5 times	Average
89 g	92 g	90 g	91 g	93 g	91 g

As a result of extracting several times in the same manner as the same amount of Bangpungtongseong Herbal Medicine, the following extract of Bangpungtongsan Diet Essential Oil was obtained.

[Comparative Example] Production of essential oil extract of Windbreak Tongseong acid by the General Extraction Method

Licorice 90g, Gilgyeong 90g, rhubarb 68g, peppermint 54g, white ginseng 90g, cheonggung 54g, 54g of the pulverized mixture mixed with 500g of ethanol 1,500g and stirred for 10 hours and then 95 ℃ in a distillation apparatus equipped with a cooling condenser After heating for 5 hours at 1530 g extract was obtained. This extract was obtained by extracting the essential oil extract of Fung Fung Tong acid by the general extraction method of 65 g using a rotary volatilizer.

TABLE 2

1 time	Episode 2	3rd time	4 times	5 times	Average
65 g	68 g	66 g	64 g	62 g	65 g

As shown in Table 1 and Table 2 of the above Examples and Comparative Examples, it can be seen that the extraction of more wind-breakable acid diet essential oil extract through the extraction method according to the present invention.

Claims

Licorice, gilyeong, rhubarb, peppermint, baekryeung, cheongung, hyunggei dry and pulverize, the first step,

A second step of feeding the supercritical fluid into the supercritical extraction tank by putting the mixture ground through the first step,

A third step of adjusting the internal pressure of the supercritical extraction tank supplied with the mixture to 200 to 500 atmospheres and adjusting the temperature to 30 to 65 ° C., and then maintaining the supercritical extraction tank for 5 to 7 hours;

After 5 to 7 hours has elapsed by reducing the internal pressure of the supercritical extraction tank and extracting the extract from the mixture stored therein comprising a fourth step of extracting the aromatic substance from the wind-proof acidic herbal medicine.
The method according to claim 1,

Method for extracting the aromatic substance from wind-permeable acid Chinese herbal medicine further comprises a fifth step of dissolving and removing the wax component and non-volatile substances contained in the extract extracted through the fourth step as a solvent.
The method according to claim 1,

The mixture is 80 to 100 parts by weight, 60 to 80 parts by weight, 40 to 60 parts by weight, 80 to 100 parts by weight, 80 to 100 parts by weight, 40 to 60 parts by weight, 40 to 60 parts by weight of liquorice A method for extracting aromatic substances from wind-permeable acid herbal medicines, characterized in that the mixture is made.
The method according to claim 1,

The supercritical fluid is carbon dioxide (CO 2 ) characterized in that the method for extracting fragrant substances from windproof acidic herbal medicines.
The method according to claim 2,

The solvent used in the fifth step is a method for extracting an aromatic substance from wind-permeable acid herbal medicine, characterized in that ethanol.