CN112972536A

CN112972536A - A Chinese medicinal composition with hair growth promoting and hair blackening effects, and its preparation method

Info

Publication number: CN112972536A
Application number: CN202110289296.3A
Authority: CN
Inventors: 田方圆; 刘永浩; 张萱; 邓聪慧; 杨巧然; 刘瑞瑶; 何智荣; 陈锐锋; 何知秋; 韩博
Original assignee: Shihezi University
Current assignee: Shihezi University
Priority date: 2021-03-18
Filing date: 2021-03-18
Publication date: 2021-06-18

Abstract

The invention relates to a Chinese medicinal composition with hair growing and blackening effects and a preparation method thereof, the Chinese medicinal composition adopts pure Chinese medicaments of radix bupleuri, semen persicae, nigella sativa, pomegranate seeds, black sesame, beet and medically acceptable auxiliary materials of menthol, tetrahydropiperine, azone and simethicone, and the Chinese medicinal composition is prepared by mixing, crushing, supercritical extraction, extraction by edible alcohol, concentration to obtain stock solution A, then adding the menthol, the tetrahydropiperine, the azone and the simethicone into alkyl glycoside, and stirring and mixing uniformly to obtain stock solution B; the composition is prepared by uniformly mixing the stock solution A and the obtained stock solution B, and has the advantages of low cost, nature, environmental protection, convenient use, simple preparation process, exact curative effect, economy and practicability. The obtained composition can be applied to affected part to stimulate melanocyte growth at the bottom of hair follicle, and make hair black and dense. By observing 100 patients, the traditional Chinese medicine composition provided by the invention is used, the cure rate is 64%, the effective rate is 95%, and the ineffective rate is 5%.

Description

A Chinese medicinal composition with hair growth promoting and hair blackening effects, and its preparation method

Technical Field

The invention relates to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition with hair growing and blackening effects and a preparation method thereof.

Background

Alopecia is a skin disease, while senile white hair is a normal physiological phenomenon. However, with the pace of life of people becoming faster and the social pressure becoming stronger, the incidence of alopecia in people is significantly increased, and people with white hair are also gradually younger. Both alopecia and canities seriously affect the external beauty of patients and also bring certain psychological burden to the patients. Therefore, the pure natural Chinese herbal compound composition with the effects of growing and blacking hair can meet the requirements of a plurality of people with alopecia and young and middle-aged people with premature graying.

According to research, alopecia diseases mainly comprise alopecia areata and seborrheic alopecia, the incidence of the alopecia areata is related to immunity, cell factors and neuroendocrine, and local immunotherapy and application of an immunosuppressant FK506 are ideal western medicine methods for treating the alopecia areata, but the alopecia diseases have recurrence problems. Seborrheic alopecia is affected by both genetics and androgens, 5-alpha reductase inhibitors and minoxidil being the currently preferred western medicine, but the former may increase the risk of developing high grade prostate cancer. As for the reason for the whitening of hair, one is that melanocytes at the bottom of hair follicles are exposed to the external environment due to diseases or damaged due to aging; the second is that there is a reduction or even a lack of melanin granules in the hair follicles at the root of the hair, or that the melanin granules are not delivered to the hair.

The existing hair transplantation operation has the side effects of bleeding, infection, damage to superficial sensory nerves, scar and the like. The hair dye has short dyeing effect, and can damage hair scales and melanocytes in hair follicle, and even induce allergic dermatitis, leukemia, cancer, etc. Therefore, the search for an effective hair-growing and hair-blackening drug is a hot point in recent years.

Uygur medicine has been used for more than 2000 years, has a unique medical theory system, and has great effect in treating difficult and complicated diseases such as skin diseases. Wherein the prescription of the siyadan hair growing tincture is a classic vitamin medicine compound prescription. The existing products such as the compound siya hair restorer in the market have the curative effects of treating alopecia, alopecia areata, scalp itch and the like.

The invention is improved based on the formula of the siyadan hair-growing tincture which consists of three medicinal materials of nigella sativa seeds, peach kernels and pomegranate seeds, peach kernels are replaced by the alpine peach kernels, and three medicinal materials of radix bupleuri, black sesame and beet are added to prepare the traditional Chinese medicine compound composition with good hair-growing and hair-blacking effects. Can provide convenience for people suffering from alopecia and premature graying of hair.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine composition with hair growth and blacking effects and a preparation method thereof aiming at the defects of the prior art, the traditional Chinese medicine composition adopts pure traditional Chinese medicines of radix bupleuri, semen persicae, nigella sativa seeds, pomegranate seeds, black sesame, beet and medically acceptable auxiliary materials of menthol, tetrahydropiperine, azone and simethicone, and is prepared by mixing, crushing, supercritical extraction, extraction with edible alcohol, concentration to obtain a stock solution A, then adding the menthol, the tetrahydropiperine, the azone and the simethicone into alkyl glycoside, and stirring and mixing uniformly to obtain a stock solution B; the composition is prepared by uniformly mixing the stock solution A and the obtained stock solution B, and has the advantages of low cost, nature, environmental protection, convenient use, simple preparation process, exact curative effect, economy and practicability. The obtained composition can be applied to affected part to stimulate melanocyte growth at the bottom of hair follicle, and make hair black and dense. By observing 100 patients, the traditional Chinese medicine composition provided by the invention is used, the cure rate is 64%, the effective rate is 95%, and the ineffective rate is 5%.

The traditional Chinese medicine composition with the effects of growing and blackening hair is prepared from, by weight, 25-45 parts of radix bupleuri, 10-23 parts of semen pruni armeniacae, 20-35 parts of nigella sativa seeds, 12-20 parts of pomegranate seeds, 10-25 parts of black sesame seeds, 3-8 parts of beet and medically acceptable auxiliary materials, namely 2-4 parts of menthol, 2-3 parts of tetrahydropiperine, 2-4 parts of azone and 5-10 parts of simethicone.

The traditional Chinese medicine composition is prepared from, by weight, 25-30 parts of radix bupleuri, 10-18 parts of semen pruni armeniacae, 20-30 parts of nigella sativa seeds, 12-15 parts of pomegranate seeds, 10-20 parts of black sesame seeds, 3-5 parts of beet and medically acceptable auxiliary materials, 2-4 parts of menthol, 2-3 parts of tetrahydropiperine, 2-4 parts of azone and 5-10 parts of simethicone.

The traditional Chinese medicine composition is prepared from 30 parts by weight of radix bupleuri, 15 parts by weight of semen persicae, 25 parts by weight of nigella sativa seeds, 12 parts by weight of pomegranate seeds, 15 parts by weight of black sesame seeds, 4 parts by weight of beet and medically acceptable auxiliary materials of 4 parts by weight of menthol, 3 parts by weight of tetrahydropiperine, 4 parts by weight of azone and 7 parts by weight of simethicone.

The preparation method of the traditional Chinese medicine composition with the effects of growing and blackening hair comprises the following steps:

a. weighing the raw materials according to the formula ratio, drying 25-45 parts of radix bupleuri, 10-23 parts of semen pruni armeniacae, 20-35 parts of nigella sativa seeds, 12-20 parts of pomegranate seeds, 10-25 parts of black sesame seeds and 3-8 parts of beet at the temperature of 80-90 ℃, and crushing by using a crusher; sieving with 200-300 mesh sieve to remove insoluble solid to obtain mixture;

b. using CO for the mixture obtained in the step a₂Extracting with a supercritical extractor under 40-70MPa at 40-60 deg.C for 180 min to obtain liposoluble fraction and residue;

c. b, adding 20-40 times of edible alcohol solution with the mass fraction of 50-95% into the medicine residues obtained in the step b, boiling for 30-60 minutes under reduced pressure, extracting for 3 times, extracting for 2-4 hours each time, and combining the extracting solutions;

d. concentrating the extracting solution obtained in the step c to the density of 1.00-1.80g/ml, and then uniformly mixing the fat-soluble part obtained in the step b with the concentrated solution to obtain a stock solution A;

e. adding 2-4 parts of menthol, 2-3 parts of tetrahydropiperine, 2-4 parts of azone and 5-10 parts of simethicone into 5-8 parts of alkyl glycoside with the polymerization degree of 1.4-1.6, the solid content of more than or equal to 50% and the density of 1.05-1.15, and uniformly mixing by using a stirrer to obtain a stock solution B;

f. and d, uniformly mixing the stock solution A obtained in the step d with the stock solution B obtained in the step e to obtain the traditional Chinese medicine composition with the functions of growing and blacking hair.

The mass fraction of the edible alcohol in the step c is 75-95%, and the adding amount is 25-30 times.

The density of the concentrated extract in the step d is 1.05-1.35 g/ml.

The invention relates to a traditional Chinese medicine composition with hair growth and blacking effects and a preparation method thereof, wherein the traditional Chinese medicine composition comprises the following raw materials:

root of Bupleurum exaltatum in Xinjiang, Bupleurum exaltatum: has effects in tranquilizing mind, prolonging sleep time, relieving fever and pain, relieving convulsion, relieving inflammation, resisting bacteria and virus, protecting liver, promoting function of gallbladder, reducing blood lipid, resisting allergy and ulcer, enhancing immunity, resisting nephritis, resisting tumor, resisting lipid peroxidation, and promoting hemolysis of saikosaponin;

semen Caryae Cathayensis (Carr) franch is dried mature seed of Prunus davidiana of Rosaceae, and semen Caryae Cathayensis: the traditional Chinese medicine composition is mainly used for promoting blood circulation and removing blood stasis, relaxing bowel, nourishing brain cells and enhancing brain function. Meanwhile, the semen pruni armeniacae contains a large amount of vitamin E, has the effects of moistening skin and blackening beard and hair, and can moisten and smooth the skin and be rich in elasticity;

the Nigella sativa seeds are dry mature seeds of Nigella glandulifera Freyn et sint, Ranunculaceae, and the Nigella sativa seeds: the nigella sativa L.seed contains nigellatin and volatile oil, and the oil mainly contains Bailixiang and nigellatin; has effects in invigorating kidney, strengthening brain, dredging channels, promoting lactation, and promoting urination;

the pomegranate seeds are seed kernels of pomegranate Punica grandiflora L, and the pomegranate seeds: the pomegranate seeds have the main effects of relieving the fragility of blood vessels, improving the skin, reducing the formation of wrinkles, improving the vision, enhancing the vitality of capillary vessels, delaying aging and the like;

black sesame is black seed of Sesamum indicum l. of the family of Sesamum, black sesame: sweet in flavor and neutral in nature. Has the functions of nourishing liver and kidney, benefiting blood, moistening intestine, relaxing bowels and promoting lactation. In addition, it also has effects of invigorating stomach, protecting liver, promoting erythrocyte growth, blackening hair, and caring skin.

Beet is the leaf of Beta vulgaris L, beet: the beet has rich calcium and iron content, and can promote bone growth and development and prevent anemia, and the betaine hydrochloride contained therein can promote digestion and absorption.

All the sources of the above substances were purchased from Xinjiang.

Detailed Description

To facilitate a better understanding of the present solution, the present invention provides the following examples.

Example 1 (base of 100 ml)

a. Weighing the raw materials according to the formula ratio in parts by weight, drying 25 parts of radix bupleuri, 23 parts of semen pruni armeniacae, 35 parts of nigella sativa, 20 parts of pomegranate seeds, 25 parts of black sesame and 8 parts of beet at the temperature of 80 ℃, crushing by using a crusher, sieving by using a 200-mesh sieve, and removing insoluble solids to obtain a mixture;

b. using CO for the mixture obtained in the step a₂Extracting with a supercritical extractor under 40MPa at 40 deg.C for 120 min to obtain liposoluble part and residue;

c. b, adding 20 times of edible alcohol solution with the mass fraction of 50% into the dregs obtained in the step b, boiling for 30 minutes under reduced pressure, extracting for 3 times, extracting for 2 hours each time, and combining the extracting solutions;

e. adding 2 parts of menthol, 3 parts of tetrahydropiperine, 4 parts of azone and 10 parts of simethicone into 5 parts of alkyl glycoside with the polymerization degree of 1.4-1.6, the solid content of more than or equal to 50% and the density of 1.05-1.15, and uniformly mixing by using a stirrer to obtain a stock solution B;

f. and d, uniformly mixing the stock solution A obtained in the step d with the stock solution B obtained in the step e to obtain the traditional Chinese medicine composition with the functions of growing and blackening hair, and then inspecting, filling and storing.

Example 2 (base of 100 ml)

a. Weighing the raw materials according to the formula ratio in parts by weight, drying 45 parts of radix bupleuri, 10 parts of semen pruni armeniacae, 20 parts of nigella sativa, 12 parts of pomegranate seeds, 10 parts of black sesame and 3 parts of beet at the temperature of 90 ℃, crushing by using a crusher, sieving by using a 300-mesh sieve, and removing insoluble solids to obtain a mixture;

b. using CO for the mixture obtained in the step a₂Extracting with supercritical extractor under 70MPa at 60 deg.C for 180 min to obtain liposoluble part and residue;

c. b, adding 40 times of edible alcohol solution with the mass fraction of 95% into the dregs obtained in the step b, boiling for 60 minutes under reduced pressure, extracting for 3 times, extracting for 3 hours each time, and combining the extracting solutions;

e. adding 4 parts of menthol, 2 parts of tetrahydropiperine, 2 parts of azone and 5 parts of simethicone into 6 parts of alkyl glycoside with the polymerization degree of 1.4-1.6, the solid content of more than or equal to 50% and the density of 1.05-1.15, and uniformly mixing by using a stirrer to obtain a stock solution B;

f. and d, uniformly mixing the stock solution A obtained in the step d with the stock solution B obtained in the step e to obtain the traditional Chinese medicine composition with the functions of growing and blacking hair. And then checking, filling and storing.

Example 3 (base of 100 ml)

a. Weighing the raw materials according to the formula ratio in parts by weight, drying 30 parts of radix bupleuri, 15 parts of semen pruni armeniacae, 25 parts of nigella sativa, 15 parts of pomegranate seeds, 20 parts of black sesame and 5 parts of beet at the temperature of 85 ℃, and crushing by using a crusher; sieving with 250 mesh sieve to remove insoluble solid to obtain mixture;

b. using CO for the mixture obtained in the step a₂Extracting with supercritical extractor under 50MPa at 50 deg.C for 160 min to obtain liposoluble part and residue;

c. b, adding 30 times of edible alcohol solution with the mass fraction of 75% into the dregs obtained in the step b, boiling for 45 minutes under reduced pressure, extracting for 3 times, extracting for 4 hours each time, and combining the extracting solutions;

e. adding 3 parts of menthol, 2 parts of tetrahydropiperine, 3 parts of azone and 8 parts of simethicone into 8 parts of alkyl glycoside with the polymerization degree of 1.4-1.6, the solid content of more than or equal to 50% and the density of 1.05-1.15, and uniformly mixing by using a stirrer to obtain a stock solution B;

Example 4 (base of 100 ml)

a. Weighing the raw materials according to the formula ratio, 25 parts of radix bupleuri, 18 parts of semen pruni armeniacae, 30 parts of nigella sativa, 15 parts of pomegranate seeds, 20 parts of black sesame and 5 parts of beet, drying at the temperature of 82 ℃, and crushing by using a crusher; sieving with 230 mesh sieve to remove insoluble solid to obtain mixture;

b. using CO for the mixture obtained in the step a₂Extracting with a supercritical extractor under 50MPa at 45 deg.C for 130 min to obtain liposoluble part and residue;

c. b, adding 25 times of edible alcohol solution with the mass fraction of 75% into the dregs obtained in the step b, boiling for 35 minutes under reduced pressure, extracting for 3 times, extracting for 3 hours each time, and combining the extracting solutions;

d. concentrating the extracting solution obtained in the step c to the density of 1.05-1.35g/ml, and then uniformly mixing the fat-soluble part obtained in the step b with the concentrated solution to obtain a stock solution A;

e. adding 2 parts of menthol, 2 parts of tetrahydropiperine, 2 parts of azone and 5 parts of simethicone into 6 parts of alkyl glycoside with the polymerization degree of 1.4-1.6, the solid content of more than or equal to 50% and the density of 1.05-1.15, and uniformly mixing by using a stirrer to obtain a stock solution B;

Example 5 (base of 100 ml)

a. Weighing the raw materials according to the formula ratio, 30 parts of radix bupleuri, 10 parts of semen pruni armeniacae, 20 parts of nigella sativa, 12 parts of pomegranate seeds, 10 parts of black sesame and 3 parts of beet, drying at the temperature of 85 ℃, and crushing by using a crusher; sieving with 250 mesh sieve to remove insoluble solid to obtain mixture;

b. using CO for the mixture obtained in the step a₂Extracting with a supercritical extractor under 60MPa at 55 deg.C for 140 min to obtain liposoluble part and residue;

c. b, adding 30 times of edible alcohol solution with the mass fraction of 95% into the dregs obtained in the step b, boiling for 50 minutes under reduced pressure, extracting for 3 times, extracting for 2 hours each time, and combining the extracting solutions;

e. adding 4 parts of menthol, 2 parts of tetrahydropiperine, 4 parts of azone and 8 parts of simethicone into 7 parts of alkyl glycoside with the polymerization degree of 1.4-1.6, the solid content of more than or equal to 50% and the density of 1.05-1.15, and uniformly mixing by using a stirrer to obtain a stock solution B;

Example 6 (base of 100 ml)

a. Weighing the raw materials according to the formula ratio, 28 parts of radix bupleuri, 14 parts of semen persicae, 25 parts of nigella sativa, 13 parts of pomegranate seeds, 15 parts of black sesame and 4 parts of beet, drying at the temperature of 90 ℃, and crushing by using a crusher; sieving with 300 mesh sieve to remove insoluble solid to obtain mixture;

b. using CO for the mixture obtained in the step a₂Extracting with a supercritical extractor under 60MPa at 60 deg.C for 180 min to obtain liposoluble part and residue;

c. b, adding 28 times of edible alcohol solution with the mass fraction of 80% into the dregs obtained in the step b, boiling for 60 minutes under reduced pressure, extracting for 3 times, extracting for 2.5 hours each time, and combining the extracting solutions;

e. adding 3 parts of menthol, 3 parts of tetrahydropiperine, 3 parts of azone and 10 parts of simethicone into 8 parts of alkyl glycoside with the polymerization degree of 1.4-1.6, the solid content of more than or equal to 50% and the density of 1.05-1.15, and uniformly mixing by using a stirrer to obtain a stock solution B;

Example 7 (base of 100 ml)

a. Weighing 30 parts of radix bupleuri, 15 parts of semen persicae, 25 parts of nigella sativa, 12 parts of pomegranate seeds, 15 parts of black sesame seeds and 4 parts of beet according to the formula ratio, drying at the temperature of 90 ℃, and crushing by using a crusher; sieving with 300 mesh sieve to remove insoluble solid to obtain mixture;

c. b, adding 40 times of edible alcohol solution with the mass fraction of 95% into the dregs obtained in the step b, boiling for 60 minutes under reduced pressure, extracting for 3 times, extracting for 4 hours each time, and combining the extracting solutions;

e. adding 4 parts of menthol, 3 parts of tetrahydropiperine, 4 parts of azone and 7 parts of simethicone into 8 parts of alkyl glycoside with the polymerization degree of 1.4-1.6, the solid content of more than or equal to 50% and the density of 1.05-1.15, and uniformly mixing by using a stirrer to obtain a stock solution B;

Example 8

The experimental results were verified for the compositions obtained in any of examples 1 to 7:

and (3) hair growth effect verification:

experimental animals: c57BL/6 mice, half male and female, clean grade, 7-8 weeks old, body weight 20-22g, total 40, provided by the animal center of Xinjiang medical university; feeding the mice in a laboratory for 7 days in a conventional way, and starting an experiment after the mice adapt to the environment;

the experimental method comprises the following steps: grouping animals, modeling and administering;

grouping: 40C 57BL/6 mice were randomly divided into five groups, namely a model group, a positive control group, a traditional Chinese medicine compound group and a blank group. The first three components are correspondingly coated with distilled water, and the baofantang six-ingredient alopecia preventing and hair growing tincture is prepared;

molding: the mice were anesthetized with ether, a paraffin/rosin mixture (1:1) was melted, smeared on the back of the mice, and after solidification and hardening, the mixture was removed. And (3) the successful standard of molding: the mouse has no abnormal expression, the surface of the back skin is smooth, no root hair and wound are preferred, the depilation area is about 2cm multiplied by 2cm, and the hair of the mouse is induced to enter the growth period from the resting period;

administration: coating corresponding medicines on the depilatory area twice a day from the next day after depilation;

detecting the apoptosis number of hair follicle cells:

at 15d after depilation, all mice were sacrificed by cervical dislocation. Fixing skin at the same part of a depilation region with 10% formaldehyde, dehydrating, embedding in paraffin, slicing, spreading on a polylysine glass slide, and performing in-situ hybridization by using an in-situ hybridization technique as an end marking method (detecting the growth/apoptosis condition of cells in hair follicles);

the statistical method comprises the following steps:

the experimental data were analyzed using statistical software SPSS 17.0, and the measured data were expressed as mean ± standard deviation and tested using the t-test method. The counting data are expressed by a rate, and the counting data are considered to have statistical significance by adopting a Chi 2 test and P is less than 0.05;

as a result:

the detection result of the apoptosis of the hair follicle cells in the hair removal area on the back of each group of mice shows that the numbers of the apoptosis of the hair follicle cells in the blank group and the model control group are respectively (41.56 +/-0.32) and (42.77 +/-0.68) per visual field at 15d after hair removal; the numbers of apoptosis of hair follicle cells of mice in the traditional Chinese medicine compound group and the positive control group are respectively (16.54 +/-0.33) and (17.32 +/-0.37) per visual field. The apoptosis number of the traditional Chinese medicine compound group and the positive control group is obviously less than that of the blank group and the model group, and P is less than 0.05, so that the statistical significance is achieved. Meanwhile, the traditional Chinese medicine compound group and the positive control group as well as the blank group and the model group have no statistical significance when P is more than 0.005. The conclusion is drawn that the traditional Chinese medicine compound has obvious inhibition effect on the apoptosis of hair follicle cells, has similar effect to the Baofitang six-ingredient alopecia preventing and hair growing tincture on the hair growing, and has no obvious correlation between the inhibition effect and the solvent. The traditional Chinese medicine composition is proved to have the hair growth effect.

Example 9

And (3) hair blackening effect verification:

the experimental method comprises the following steps: animal grouping, modeling, administration:

grouping: 40C 57BL/6 mice were randomly divided into four groups, namely a model group, a positive control group, a Chinese medicinal compound group and a blank group. The first three components are coated with distilled water and polygonum multiflorum extracting solution correspondingly, and the blank group is not coated with any medicine;

molding: anesthetizing the mouse with ether, heating and melting paraffin/rosin mixture (1:1), smearing on the back of the mouse, solidifying and hardening, and removing the mixture; 12h after unhairing, selecting a mouse without a wound on the back, smearing a hydrogen peroxide solution with the concentration of 6% on the unhairing position, 2 times a day for 7d, and making a model successfully according to the standard: the mouse has no abnormal expression, white villi grow on the surface of back skin, no black miscellaneous hair, no black skin and wound are preferably on the back, the depilation area is about 2cm multiplied by 2cm, melanocytes in hair follicles are damaged, and light-colored hair is induced to grow;

administration: respectively coating corresponding medicines on the hair growing areas with light color twice a day after the modeling is successful;

and (3) recovery detection of hair follicle melanocytes:

15d after the modeling is successful, all mice are killed by a cervical dislocation method, the skin at the same position of a depilation region is taken, 10% formaldehyde is used for fixing, dehydration and paraffin embedding are carried out, two longitudinal sections and two transverse sections of the skin are taken for each section, HE dyeing is respectively carried out, and the distribution condition of melanin of each group of the longitudinal sections is observed under a microscope;

the statistical method comprises the following steps:

as a result:

the results of the back longitudinal section of each group of mice show that the number of melanin hair follicles in the blank group is (110.43 +/-16.22) per visual field, the number of melanin hair follicles in the traditional Chinese medicine compound group is (133.05 +/-11.52) per visual field, the number of melanin hair follicles in the positive control group is (134.77 +/-12.09) per visual field, and the number of melanin hair follicles in the model group is (45.44 +/-9.02) per visual field; compared with the traditional Chinese medicine compound group, the blank group has statistical significance when P is less than 0.05; comparing the blank group with the positive control group, wherein P is less than 0.01, and the statistical significance is achieved; compared with the model group, the blank group has no statistical significance when P is more than 0.05; compared with the positive control group, the traditional Chinese medicine compound group has statistical significance when P is less than 0.01. The conclusion is that the traditional Chinese medicine compound has obvious promotion effect on the growth of the melanocyte of the hair follicle, is similar to the effect of the polygonum multiflorum extracting solution on blacking the hair, and has no obvious relationship between the promotion effect and the solvent. The traditional Chinese medicine composition is proved to have the hair blacking effect.

Typical cases (the following experiments were performed with the patient's own consent):

case 1:

mr. Wang, Man, age 64, Xinjiang Shihe university, diagnosed hair loss, thinning, white hair. Symptoms and signs (clinical manifestations) are that the hair is shed and sparse ten years ago, and the hair is whitened to more than 70%; many treatments have not been effective and have become more serious over the years. After the traditional Chinese medicine composition prepared in the example 2 is used for 4 months in 2020, the hair is normally cleaned, and then the hair is smeared on the root of the hair, and is properly massaged to promote absorption, 1 time in two days with the dosage of about 4-5ml each time, and the hair does not need to be washed after use, and is naturally dried in the air, so that the hair is kept on the scalp and the nutrition of the hair root is kept. After the hair is continuously used for one month, the hair is not dropped, white hair is slowly turned into black, the black hair is developed by 80 percent, beard is also turned black, eyebrow is also grown, and the hair is turned black and thickened.

Case 2:

zhao teacher, man, 56 years old, Shihe university, pharmacist, diagnosed baldness and canities in 9 months of 2020. Signs of symptoms (clinical manifestations): baldness, white hair; the effect cannot be achieved by self-medicine preparation treatment, and the symptoms become more serious after various hair growing and hair blackening shampoos are replaced. The traditional Chinese medicine composition provided in example 2 is used in 9 months in 2020, after hair is normally washed, the hair is smeared on the root of the hair, and is properly massaged to promote absorption, the dosage is about 4-5ml every two days, hair washing is not needed after the hair is used, the hair is naturally dried in the air, so that the hair is remained on the scalp and the nutrition of the root of the hair is kept, 70% of white hair is changed into black hair after one month, the bald hair is tiny and black, and the bald hair is basically full of black hair after the hair is continuously used for two months.

Case 3:

the hair is white, the study pressure is huge, and the white hair accounts for about one third of the whole hair after being usually left up all night; many medicines have no effect when being taken orally or externally used as hair blackening liquid; after the traditional Chinese medicine composition provided by the embodiment 2 is used, the hair is normally cleaned, the hair is smeared on the root of the hair, and is properly massaged to promote absorption, the dosage is about 4-5ml every time after 1 time every two days, the hair does not need to be washed after use, the hair is naturally dried in the air, the hair is kept on the scalp and the nutrition of the hair root is kept, the white hair disappears after 2 months, and the hair does not relapse at present after follow-up.

By observing 100 patients, the traditional Chinese medicine composition provided by the invention is used, the cure rate is 64%, the effective rate is 95%, and the ineffective rate is 5%.

Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention as defined in the appended claims.

Claims

1. The traditional Chinese medicine composition with the effects of growing and blackening hair is characterized by being prepared from 25-45 parts by weight of radix bupleuri, 10-23 parts by weight of semen pruni armeniacae, 20-35 parts by weight of nigella sativa seeds, 12-20 parts by weight of pomegranate seeds, 10-25 parts by weight of black sesame seeds, 3-8 parts by weight of beet and 2-4 parts by weight of medically acceptable auxiliary materials, namely menthol, 2-3 parts by weight of tetrahydropiperine, 2-4 parts by weight of azone and 5-10 parts by weight of simethicone.

2. The traditional Chinese medicine composition with the effects of growing and blacking hair according to claim 1, which is prepared from 25-30 parts by weight of radix bupleuri, 10-18 parts by weight of semen pruni armeniacae, 20-30 parts by weight of nigella sativa seeds, 12-15 parts by weight of pomegranate seeds, 10-20 parts by weight of black sesame seeds, 3-5 parts by weight of beet and 2-4 parts by weight of pharmaceutically acceptable auxiliary materials such as menthol, 2-3 parts by weight of tetrahydropiperine, 2-4 parts by weight of azone and 5-10 parts by weight of simethicone.

3. The traditional Chinese medicine composition with the effects of growing and blacking hair according to claim 1, which is prepared from 30 parts by weight of radix bupleuri, 15 parts by weight of semen pruni armeniacae, 25 parts by weight of nigella sativa seeds, 12 parts by weight of pomegranate seeds, 15 parts by weight of black sesame seeds, 4 parts by weight of beet and 4 parts by weight of medically acceptable auxiliary materials such as menthol, 3 parts by weight of tetrahydropiperine, 4 parts by weight of azone and 7 parts by weight of simethicone.

4. The preparation method of the traditional Chinese medicine composition with the effects of growing and blacking hair according to claim 1 is characterized by comprising the following steps:

e. adding 2-4 parts of menthol, 2-3 parts of tetrahydropiperine, 2-4 parts of azone and 5-10 parts of simethicone into 5-8 parts of alkyl glycoside with average polymerization degree of 1.4-1.6, solid content of more than or equal to 50% and density of 1.05-1.15, and uniformly mixing by using a stirrer to obtain stock solution B;

5. The method for preparing a Chinese medicinal composition for promoting hair growth and blackening hair according to claim 4, wherein the edible alcohol in step c is added in an amount of 25-30 times at a mass fraction of 75-95%.

6. The method for preparing a Chinese medicinal composition for promoting hair growth and blackening hair according to claim 4, wherein the concentration of the extractive solution in step d is 1.05-1.35 g/ml.