KR101661120B1 - Perfume composition for awakening effect or enhancing ability to concentration comprising essential oil of Magnolia Flos as an effective component - Google Patents

Abstract

The fragrance composition containing the essential oil of the present invention as an active ingredient increases the emotional comfort of the brain and brings the fragrance composition to a highly concentrated state by awakening the fragrance composition so that the fragrance composition for enhancing the awakening effect or the concentration ability or the cosmetic composition containing it, Can be used as a composition for external application for skin.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a fragrance composition for enhancing an arousal effect or a concentration effect,

The present invention relates to a perfume composition for enhancing concentration ability containing natural essential oil as an active ingredient, or a composition for external application for skin such as a cosmetic, a soap or a bathing agent containing the above-mentioned antioxidant composition.

Human brain waves are classified into three groups according to the frequency range: delta (0.5-3 Hz), theta (4-7 Hz), alpha (8-13 Hz), beta (14-30 Hz) (gamma; 30 Hz or more).

The delta wave is an irregular wave corresponding to a frequency of 0.5 to 3 Hz. It is predominantly at deep sleep and appears awake when abnormalities such as epilepsy, brain tumor, and mental retardation are present.

Cetaphas are regular waves of frequencies between 4 and 8 Hz, usually recorded in the occipital lobe and temporal lobe. It involves deeply internalized, silent physical, emotional and thought activities. It usually occurs when you are sleepy, not in a deep sleep state, and it is great when you are hurt, artistic, and enjoyable and joyful at work or play.

Alpha waves are the basis of brain waves and correspond to frequencies between 8 and 13 Hz. (7 ~ 8Hz), mid - alpapa (9 ~ 11Hz), fast alfapar (12 ~ 12Hz), and normal alfalfa (12 ~ 13Hz).

The slow alpha wave is a state of the brain that is relaxed, sleeps, or rests, and the mid-alpha wave is characterized by the fact that it is not normally accompanied by stress, and it is mediated, creative activity, And fast alpha is present when you have to cope with a stressful concentration in an urgent or dangerous state and are extremely concentrated in one job so you can not afford to think of the rest and do not respond to other signals or stimuli.

Beta waves are 13 to 30 Hz of frequency, and they appear when doing normal work or conscious actions and are activated when there is mental activity such as information processing. It is also divided into low-beta waves (12 to 15 Hz), mid-beta waves (15 to 20 Hz) and high-beta waves (20 to 30 Hz).

The low-beta is sensory motor rhythm (SMR) parasympathetic, and the motion sensation without body movements. When minimizing the activity of the cortex, it becomes preponderant when performing a relatively simple task, and the mid- High-beta waves become dominant when performing relatively complex reasoning or complex information processing activities, or emotional tension, anxious excitability.

Gamma waves correspond to 30 to 50 Hz, and are the fastest of the brain activity waves, emotionally more irritable, or active at higher cognitive functions such as reasoning and judgment. In addition, the relative gamma power spectrum value increases in the parietal lobe when attention is drawn by visual stimulation or cognitive activity.

On the other hand, Magnolia Flos is a bud of Magnoliaceae, a deciduous tree of the deciduous tree with dicotyledonous plants. It is known as Magnolia biondii Pampanini, Magnolia denudata Desrousseaux, Magnolia kobus De Candolle, and Magnolia sprengeri Pampanini. The nature is warm, poisonous, and flavorful. 12 Gyeongmyeong into the menopause and the duodenal gyeongpunggyeong (祛风 通竅) to demonstrate the efficacy of a headache, noseun (鼻 臓, rhinitis), color (nasal obstruction, nasal obstruction), biliary stomach 鼻 流 涕 涕, etc. Lt; / RTI > Shin is known as an excellent medicinal herb to treat rhinitis.

In the Korean Patent Laid-Open Nos. 10-2012-0123626 and 10-2012-0123627, Shin extract is used as an active ingredient of foods or medicines and inhibits osteoclast formation and osteoclast-induced bone resorption, In Korean Patent Laid-Open No. 10-2012-0122439, it is described that the ethanol extract of Shin, has the effect of treating pancreatic cancer. In Korean Patent No. 10-0207959, A compound of the present invention has a therapeutic effect on a disease of a vascular disease.

In addition, Korean Patent Laid-Open Publication No. 10-2006-0082205 discloses that Shin extract can be used as a whitening cosmetic through inhibition of tyrosinase activity and melanin formation inhibitory activity, Korean Patent No. 10-0680845 discloses that a granulocyte, a polysaccharide extracted from a ginseng, can be used as cosmetics through skin cell proliferation and skin moisturizing effect.

However, Korean Patent Laid-Open Nos. 10-2012-0123626, 10-2012-0123627 and 10-2012-0122439 demonstrate functional or medical efficacy through oral ingestion, and it is known that Shin is not an essential oil, Korean Patent No. 10-0207959 is an invention using a lycarin compound separated and purified from Shinyi, Korean Patent Laid-Open Nos. 10-2006-0082205 and Korean Patent No. 10-0680845 are used as external preparations for skin However, it does not refer to the effect of improving the arousal effect or concentration by using a compound in which the ginseng is extracted from the extract or ginseng extract, rather than the essential oil.

It is an object of the present invention to provide a perfume composition for enhancing an arousal or concentration of concentration, which exhibits an awakening effect while increasing the emotional comfort of the brain from essential oils of natural plants and achieves a highly concentrated state.

It is also an object of the present invention to provide an external preparation for skin comprising the above-mentioned perfume composition.

In order to accomplish the above object, the present invention provides a perfume composition for improving concentration using an essential oil of Magnolia Flos as an active ingredient.

In the present invention, essential oil refers to volatile organic compounds extracted from leaves, stems, fruits, roots and flowers of plants by various methods.

The essential oil according to one embodiment of the present invention may be one extracted from a god, i.e., a magnolia and a bud drying sample.

The essential oil according to one embodiment of the present invention can be extracted by various known extraction methods such as steam distillation method, cold precipitation method, organic solvent method and supercritical extraction method, and supercritical extraction method is preferably used. The recovery rate of the essential oil is 0.01% or less when Shin extracts the essential oil by the steam distillation method. However, when the supercritical extraction method is used, the recovery rate of the essential oil increases by at least 10 to 50 times as compared with the steam distillation method.

In the case of supercritical extraction, the extraction yield can be increased by using CO ₂ as the extraction solvent at 30 to 75 ° C, preferably 35 to 50 ° C and 150 to 500 bar, preferably 350 to 450 bar .

The essential oil according to one embodiment of the present invention is not particularly limited but may be expressed as a combination of balsamic, spicy, earthy, and herbal scents, balsamic and spicy.

The relative beta power spectrum (RB), the relative gamma power spectrum (RG) and the ratio of SMR to theta (RST) , And the ASM (spectral edge frequency 50% of the alpha spectrum band) to increase the emotional comfort of the brain by increasing the RSMT (ratio of (SMR ~ mid beta) to theta), SEF50 (spectral edge frequency 50% Bringing them to a highly concentrated state.

The fragrance composition according to one embodiment of the present invention contains the essential oil as an active ingredient, and may be a perfume or an orienting agent.

In the perfume composition according to an embodiment of the present invention, the essential oil may be contained in an amount of 0.01 to 100% by weight, preferably 0.1 to 99% by weight, more preferably 1 to 90% by weight.

The present invention provides an external preparation for skin comprising the above-mentioned perfume composition.

The composition for external application for skin is not particularly limited, but may be a cosmetic, a soap or a bathing agent.

The perfume composition or composition for external application for skin of the present invention may further comprise an appropriate carrier, excipient or diluent commonly used in the production of perfume, fragrance or cosmetic, soap, bathing agent and the like.

Examples of the carrier, excipient or diluent include purified water, oil, wax, fatty acid, fatty acid alcohol, fatty acid ester, surfactant, humectant, thickening agent, antioxidant, viscosity stabilizer, , Buffers, lower alcohols, and the like, but are not limited thereto. If necessary, whitening agents, moisturizing agents, vitamins, ultraviolet screening agents, perfumes, dyes, and antimicrobial agents may be included.

Examples of the wax include beeswax, wax, carnauba, candelilla, montan, ceresin, liquid paraffin, lanolin, and the like. Examples of the oil include hydrogenated vegetable oil, pharmacopoeia, cottonseed oil, olive oil, palm oil, jojoba oil and avocado oil. Can be used.

As the fatty acid, stearic acid, linoleic acid, linolenic acid and oleic acid may be used. As the fatty acid alcohol, cetyl alcohol, octyldodecanol, oleyl alcohol, panthenol, lanolin alcohol, stearyl alcohol and hexadecanol may be used As fatty acid esters, isopropyl myristate, isopropyl palmitate, and butyl stearate may be used.

Examples of the surfactant include anionic surfactants such as sodium stearate, sodium cetyl sulfate, polyoxyethylene lauryl ether phosphate and sodium N-acyl glutamate; Cationic surfactants such as stearyldimethylbenzylammonium chloride and stearyltrimethylammonium chloride; Amphoteric surfactants such as alkylaminoethylglycine hydrochloride and lecithin; Glycerin monostearate, sorbitan monostearate, sucrose fatty acid ester, propylene glycol monostearate, polyoxyethylene oleyl ether, polyethylene glycol monostearate, polyoxyethylene sorbitan monopalmitate, polyoxyethylene coconut fatty acid monoethanol Nonionic surfactants such as monoethanol arnide, polyoxypropylene glycol, polyoxyethylene castor oil, polyoxyethylene lanolin, and the like.

As the moisture absorbent, glycerin, 1,3-butylene glycol, propylene glycol may be used, and as the lower alcohol, ethanol and isopropanol may be used. Examples of thickeners include sodium alginate, sodium caseinate, gelatin agar, xanthan gum, starch, cellulose ethers (e.g., hydroxyethylcellulose, methylcellulose, carboxymethylcellulose, hydroxypropylmethylcellulose), polyvinylpyrrolidone, Polyvinyl alcohol, polyethylene glycol, sodium carboxymethyl cellulose, and the like.

Examples of the antioxidant include butylated hydroxytoluene, butylated hydroxyanisole, propyl gallate, citric acid, and ethoxyquin; chelating agents include disodium edetate, ethanedioxy diphosphate Is available.

As the buffer, citric acid, sodium citrate, boric acid, borax, and disodium hydrogen phosphate can be used.

However, it is not limited to the above-mentioned carrier, excipient or diluent.

Formulations of a perfume composition or a composition for external application for skin according to an embodiment of the present invention may be formulated as a solution, a suspension, an emulsion, a lotion, a cream, an emulsion, a soap, a surfactant-containing cleansing oil, But are not limited to, an image, a suspension, an emulsion, a cream, a gel, a spray, a papermaking, an alarm, a patch,

The amount of the perfume composition of the present invention contained in the composition for external application for skin may be appropriately selected and blended according to the conventional techniques in the art and in order to achieve the desired effects.

In the composition for external application for skin of the present invention, the perfume composition may be contained in an amount of 0.001 to 20% by weight, preferably 0.01 to 10% by weight, based on the total weight of the cosmetic composition. If the content is below the lower limit, the desired effect can not be obtained. If the content is above the upper limit, there is a problem that the palatability to the fragrance is poor.

However, the content of the perfume composition in the composition for external application for skin corresponds to one embodiment, and can be appropriately adjusted according to the characteristics of the formulation to be applied or the desired effect.

The essential oil of the present invention, when inhaled through the nose, increases the emotional comfort of the brain and brings about an arousal effect and leads to a highly concentrated state. Therefore, a perfume composition for enhancing the alertness or concentration of perfume or fragrance, A cosmetic, a soap or a bathing agent.

Figure 1 shows the electrode attachment site of the International 10-20 EEG system.
2 is a photograph of a testee wearing a test cap for EEG test.
FIG. 3 is a brain map showing the change of AA index before and after inhalation of fragrance of essential oil.
FIG. 4 is a brain map showing the change of RB index before and after inhalation of fragrance of essential oil.
FIG. 5 is a brain map showing the change in RG index before and after inhalation of fragrance of essential oil.
FIG. 6 is a brain map showing the change in RLB index before and after inhalation of fragrance of essential oil.
FIG. 7 is a brain map showing the change in RHB index before and after inhalation of fragrance of essential oils.
8 is a brain map showing the change in RST index before and after inhalation of fragrance of essential oil.
FIG. 9 is a brain map showing the change of RSMT index before and after inhalation of fragrance of essential oil.
FIG. 10 is a brain map showing the change of the SEF 50 index before and after the inhalation of fragrance of the essential oil.

The present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the embodiments.

Production Example 1: Preparation of Shin-EI Oil by Steam Distillation

Shin-ee is a subsidiary of Magnolia ( Magnolia) kobus De Candolle) were stored at room temperature.

5 L of distilled water was added to the steam generation site of the steam distillation apparatus (Hanil LabTech., Korea), and 1000 g of the sample was placed in the refinery area. Cooling water of -4 ° C. was continuously flowed in the cooling tube. The vapors were extracted from the vapors while the steam was kept at 100 ° C for 1 hour. The vapors were extracted from the vapors and the extracted vapors were condensed in a cooling tube and classified as distilled water and essential oil . The oil layer was passed through a triangular funnel containing anhydrous sodium sulfate to remove water and stored in a refrigerator at 4 ° C until analysis.

The essential oil extracted from 1000 g of the dried gins was 2.73 g and the oil recovery was 0.27%.

Production Example 2: Preparation of Shin-keiri Oil by Supercritical Extraction

The dried sample (150 g) of Magnesium (Magnoblast bud) used in Preparation Example 1 was pulverized using a grinding machine (Hanil, Korea) and placed in a sample container of a supercritical extractor (ILSHIN, Korea) And the essential oil was extracted for 1 hour while flowing supercritical CO ₂ .

Table 1 below shows the flow rate, recovery rate, and properties of the purified oil extracted from Shin while changing the pressure to 400 bar and changing the temperature to 40 to 70 ° C.

Temperature (℃) Pressure (bar) Constant flow rate (g) Refinery recovery rate (%) Appearance 40 400 0.401 0.267 concrete 50 400 - - concrete 60 400 0.176 0.117 Oil + concrete 70 400 0.300 0.200 concrete

At 40, 50 and 60 ° C, when the pressure was lowered to 300 bar or less, the essential oil component could not be extracted. Therefore, when the temperature was fixed at 70 ° C and the pressure was changed to 200 to 400 bar, , Recoverability and properties are shown in Table 2.

Temperature (℃) Pressure (bar) Constant flow rate (g) Refinery recovery rate (%) Appearance 70 200 0.275 0.183 oil 70 300 0.375 0.250 oil 70 400 0.300 0.200 oil

The recovery of essential oils from the crude extracts of Preparation Examples 1 and 2 was highest when steam distillation at 100 ° C was carried out but the flavor evaluation was conducted by a professional group of Rose of Sharon As a result, the essential oil prepared by the steam distillation method of Preparation Example 1 was judged to be insufficient in the fragrance of the volatile component in the original fragrance of the essential oil, 400 bar supercritical extraction essential oil was selected.

Experimental Example 1: Estimation of fragrance of the essential oil

The supernatant extracted from the supercritical extract at 40 ° C and 400 bar of Preparation Example 2 was subjected to a rosso-sharon specialist group (7 persons) .

The fragrance oil was soaked in a reclaimed paper (0.5 cm ㅧ 13 cm) using a sprayer, perfumed at a position of 5 cm from the nose, described using perfume evaluation terminology, and only common fragrance was collected. The results are shown in Table 3 Respectively. The scent type used in Rose of Sharon is aldehyde, animalic, balsamic, citrus, coniferous, earthy, floral, fruity, green, herbal, medicinal, minty, marine, mossy, musky, oily, oriental, powdery, smoky, spicy, sweet, and woody, and the fragrance intensity was generally from 0 to 10.

Drape type Intensity of fragrance Drape type Intensity of fragrance Aldehyde 0 Minty 0 Animalic 0 Marine 0 Balsamic 8 Wet 0 Citrus 0 Musky 0 Coniferous 0 Oily 0 Earthy 5 Oriental 0 Floral 0 Powdery 0 Fruity 0 Smoky 0 Green 0 Spicy 7 Herbal 4 Sweet (sweet) 0 Medicinal 0 Woody 0

The essential oils of the present invention can be expressed as a combination of balsamic, spicy, earthy and herbal fragrances, and particularly strong in balsamic and spicy flavors .

Experimental Example 2: EEG analysis of the essential oil

The effect of supernatant extracted at 40 ° C and 400 bar of Preparation Example 2 on human brain waves was analyzed by electroencephalography. The instrument used for EEG was a QEEG-8 system (LXE3208, Laxa, Korea). EEG signals were measured using a data collection device and an analysis program (Telescan).

EEG measurements were carried out in a stable room with a maximum noise level of 32.5 m ² , maintaining a constant temperature (23 ^° C) and humidity (50%). The subjects were 18 men and 20 women in their 20s The mean age of the patients was 25.9 ± 1.9 years. EEG analysis was carried out using 10 standard international standard electrode systems, and 8 pre-echocardiographic (Fp1, Fp2), frontal (F3, F4), temporal (T3 , T4), parietal lobes (P3, P4), and one electrode was attached to the left and right ear lobes. The odd numbers are assigned to the left side and the even numbers are assigned to the right side at the attachment sites. The 10-20 international standard electrode attachment site for EEG is shown in FIG. A photograph of the subject having the analytical cap for EEG is shown in FIG.

The god contained in the vial was kept at a distance of 5 cm from the subject's nose by taking the essential oil on the back of the subject, and the subjects were measured before the incense-inhalation (unstimulated) and in the aspirated state (incense-stimulated) for 1 minute. Only EEG signals were measured for 30 seconds during 1 minute measurement of EEG. Data were processed by batch processing and corresponding sample T test was performed using statistical processing program of SPSS version 18.0.

Table 4 shows the meanings of the EEG analysis, the analysis waveband or the calculation formula and the analysis index used in the EEG analysis used to confirm the awakening effect or the concentration improvement.

division Analysis index Full name of EEG indicator Waveband (Hz) Meaning of the analysis index One AA Absolute Alpha Power Spectrum 8-13 Relaxation, stability 2 RB Relative Beta Power Spectrum (13 to 30) / (4 to 50) Tension, arousal, activity, cognitive function 3 RG Relative Gamma Power Spectrum (30 to 50) / (4 to 50) A high degree of cognitive function 4 RST Ratio of SMR to Theta (12 to 15) / (4 to 8) Ability of attention 5 RSMT Ratio of (SMR ~ Mid Beta) to Theta (12 to 20) / (4 to 8) EEG concentration indicator 6 SEF50 Spectral Edge Frequency 50% 4 to 50 EEG arousal 7 ASEF Spectral Edge Frequency 50% of Alpha Spectrum Band 8-13 Emotional comfort

One) AA ( absolute theta power spectrum ) Indicator Analysis

The AA index refers to the absolute power spectrum value of the absolute alpha wave region of 8 to 13 Hz in the EEG spectrum. The alpha wave is a fundamental wave reflecting neurophysiologically stable state of the brain. The appearance of alpha wave during meditation means a comfortable brain state with reduced stress and anxiety, and is used as a measure of emotional stability.

The results of measurement of the AA indicator before and after inhalation of the essential oil fractions are shown in Table 5 and FIG.

Attachment site Suction Average Standard Deviation t-test Fp1 Before inhalation 35.542 28.334 .598 After inhalation 34.121 Fp2 Before inhalation 34.649 27.945 .788 After inhalation 32.844 F3 Before inhalation 45.378 33.591 .747 After inhalation 43.023 F4 Before inhalation 44.730 34.064 .726 After inhalation 42.255 T3 Before inhalation 18.970 12.446 1.574 After inhalation 17.202 T4 Before inhalation 13.483 9.493 .642 After inhalation 12.762 P3 Before inhalation 57.470 60.283 2.209 After inhalation 48.088 P4 Before inhalation 53.972 50.606 1.399 After inhalation 47.992

Table 5 shows that the mean value of AA indexes decreased at all electrode attachment sites. Especially, P3 (left parietal lobe) showed statistically significant decrease in AA mean value (p <0.05). Such a change in AA index is expressed in a brain map and shown in FIG. After the inhaling of the essential oil, the purple area of the left parietal lobe (P3) was changed to yellow.

Therefore, it was confirmed that God awakens the brain by decreasing ALPHA after inhalation.

2) Relative theta power spectrum (RB)

The RB index refers to the spectral value obtained by dividing the absolute power of the beta wave (13-30 Hz) region by the ratio based on the absolute power of the entire frequency band. The RB index increases in the parietal lobe Respectively.

Table 6 and FIG. 4 show the measurement results of the RB indicator before and after the inhalation of essential oils.

Attachment site Suction Average Standard Deviation t-test Fp1 Before inhalation .161 .065 -1.728 After inhalation .170 Fp2 Before inhalation .168 .090 -1.527 After inhalation .180 F3 Before inhalation .159 .071 -2.105 After inhalation .171 F4 Before inhalation .149 .073 -1.058 After inhalation .156 T3 Before inhalation .261 .119 -1.659 After inhalation .276 T4 Before inhalation .242 .119 -2,290 After inhalation .258 P3 Before inhalation .186 .088 -.448 After inhalation .190 P4 Before inhalation .182 .092 -.179 After inhalation .184

Table 6 shows that the average value of the RB indexes increased at all electrode attachment sites, and the RB mean value of the EEG analysis was statistically significantly increased in F3 (left frontal lobe) and T4 (right temporal lobe) (p < 0.05). Such a change in the RB index is expressed as a brain map and is shown in FIG. Although the left frontal lobe (F3) area is not easily visible after gypsy inhalation, it can be seen that the right temporal lobe (T4) is reddish yellow.

Therefore, it can be confirmed that after the inhaling of the essential oil by God, the beta wave is increased and the state is concentrated.

3) Relative gamma power spectrum (RG)

The RG index is the absolute power ratio of the gamma wave band to the absolute power of the entire frequency domain in the EEG spectrum, which is the spectral value divided by the gamma wave region (30 to 50 Hz) into the entire frequency region (4 to 50 Hz) It belongs to the fastest wave in the wave, and it is activated when it is in an emotionally irritated state, or in a high cognitive function such as reasoning or judgment.

Table 7 and FIG. 5 show the measurement results of the RG indicator before inhalation of the sanjoin essential oil and during inhalation.

Attachment site Suction Average Standard Deviation t-test Fp1 Before inhalation .085 .072 -1.053 After inhalation .091 Fp2 Before inhalation .091 .097 -.425 After inhalation .093 F3 Before inhalation .040 .024 -2.569 After inhalation .049 F4 Before inhalation .036 .023 -1.703 After inhalation .041 T3 Before inhalation .122 .094 -.632 After inhalation .130 T4 Before inhalation .128 .086 -.399 After inhalation .134 P3 Before inhalation .044 .030 -1.002 After inhalation .049 P4 Before inhalation .041 .029 -.198 After inhalation .041

According to Table 7, the average value of RG index was not changed in the left prefrontal cortex (Fp1) and left parietal lobe (P3), but the average value of RG index increased in the rest of the electrode attachments. Especially, in Fp2 (right prefrontal cortex) The mean value of RG as an index of EEG analysis was statistically significantly increased (p <0.05). Such a change in the RG index is expressed as a brain map and is shown in FIG. In FIG. 5, although it is not easy to visually confirm the change in the region of the right prefrontal (Fp2) region, it can be confirmed that the change is significantly increased by using a statistical processing program.

Therefore, it was confirmed that the gamma wave was increased after inhaling the Sanjoin essential oil to become awake.

4) Ratio of SMR to theta

The RST index is a spectral value obtained by dividing the SMR wave range (12 to 15 Hz) with the SMR wave / theta wave ratio in the EEG spectrum into the Eustachian range (4 to 8 Hz) in the EEG spectrum and is an indicator of the EEG Is used.

Table 8 and FIG. 6 show the measurement results of the RST indicator before and after inhalation of the fragrance.

Attachment site Suction Average Standard Deviation t-test Fp1 Before inhalation .215 .168 -1.249 After inhalation .248 Fp2 Before inhalation .224 .197 -1.181 After inhalation .269 F3 Before inhalation .196 .169 -1.528 After inhalation .238 F4 Before inhalation .197 .198 -1.083 After inhalation .228 T3 Before inhalation .299 .164 -2.336 After inhalation .355 T4 Before inhalation .275 .133 -1.981 After inhalation .375 P3 Before inhalation .342 .236 -1.567 After inhalation .409 P4 Before inhalation .353 .263 -1.060 After inhalation .419

According to Table 8, the average value of RST index increased at all electrode attachment sites, and the mean value of RST, which is the index of the SMR wave in the TT (left temporal lobe), was statistically significant (p < 0.05). Such a change in the RST index is expressed in a brain map and is shown in FIG. The left temporal lobe (T3) after the inhaling of the essential oil can be seen to change from light green to yellow.

Therefore, it was confirmed that the concentration state was activated after the inhaling of essential oils.

5) Ratio of SMR to mid-beta to theta (RSMT)

The RSMT index is the spectral value obtained by dividing the SMR ~ mid beta wave range (12 ~ 20Hz) by theta wave range (4-8Hz) with the SMR ~ mid beta / theta ratio in the EEG spectrum and the neurophysiologic EEG concentration is the SMR wave mid beta-wave rhythm ratio, and it has been reported that increasing the SMR wave and the mid-beta wave increase the concentration as the theta wave decreases.

The measurement results of the RSMT indicator before and after inhalation of the essential oil fractions are shown in Table 9 and FIG.

Attachment site Suction Average Standard Deviation t-test Fp1 Before inhalation .499 .229 -1.453 After inhalation .554 Fp2 Before inhalation .518 .260 -1.608 After inhalation .611 F3 Before inhalation .462 .233 -2.119 After inhalation .535 F4 Before inhalation .444 .249 -1.624 After inhalation .507 T3 Before inhalation .818 .538 -2.242 After inhalation .939 T4 Before inhalation .827 .606 -1.854 After inhalation 1.107 P3 Before inhalation .723 .324 -.770 After inhalation .773 P4 Before inhalation .715 .358 -.672 After inhalation .767

Table 9 shows that the mean value of the RSMT index was increased at all electrode attachment sites. In particular, the mean value of RSMT, an index of EEG analysis of SMR ~ mid beta / theta waves, was statistically significant in F3 (left frontal lobe) and T3 (left temporal lobe) (P <0.05). This RSMT indicator change is depicted in FIG. 7 as a brain map. After the inhaling of the essential oil, the left frontal lobe (F3) has a light purple color and the left temporal lobe (T3) has a yellowish green color.

Therefore, it was confirmed that the concentration state was activated after the inhaling of essential oils.

6) Analysis of SEF50 (Spectral edge frequency 50%)

The spectral edge frequency (SEF) index is a method of quantifying how much the power spectrum distribution of the EEG is deflected toward the high frequency as compared with the low frequency, and is used as the area ratio to the entire frequency domain. The value of the area up to the value represents 50% of the total frequency. It is used to evaluate EEG by brain activity.

Table 10 and Fig. 8 show the measurement results of the SEF50 indicator before and after inhalation of the sanjoin essential oil.

Attachment site Suction Average Standard Deviation t-test Fp1 Before inhalation 10.650 3.280 -.648 After inhalation 10.884 Fp2 Before inhalation 11.422 4.490 -1.746 After inhalation 11.978 F3 Before inhalation 9.766 1.306 -1.161 After inhalation 10.056 F4 Before inhalation 9.734 1.214 -1.129 After inhalation 10.200 T3 Before inhalation 12.041 4.406 -2.341 After inhalation 12.881 T4 Before inhalation 12.466 5.076 -1.364 After inhalation 12.916 P3 Before inhalation 10.416 1.595 -.370 After inhalation 10.500 P4 Before inhalation 10.409 1.488 -053 After inhalation 10.425

Table 10 shows that the average value of the SEF50 index was increased at all electrode attachment sites, and the SEF50 average value of the EEG in the T3 (left temporal lobe) was statistically significantly increased (p <0.05). This SEF50 indicator change is schematically shown in FIG. 8 as a brain map. It can be seen that the left temporal lobe (T3) changed to yellow purple after the inhaling of essential oil.

Therefore, it was confirmed that EEG increased after inhalation of Sanjoin essential oil.

7) ASEF (Spectral edge frequency 50% of alpha spectrum band)

The ASEF index is an EEG indicator that shows comfort with ALPA's SEF50 (50% spectral edge frequency).

The results of measurement of ASEF indices before and after inhalation of the essential oil fractions are shown in Table 11 and FIG.

Attachment site Suction Average Standard Deviation t-test Fp1 Before inhalation 10.650 3.280 -.648 After inhalation 10.884 Fp2 Before inhalation 11.422 4.490 -1.746 After inhalation 11.978 F3 Before inhalation 9.766 1.306 -1.161 After inhalation 10.056 F4 Before inhalation 9.734 1.214 -1.129 After inhalation 10.200 T3 Before inhalation 12.041 4.406 -2.341 After inhalation 12.881 T4 Before inhalation 12.466 5.076 -1.364 After inhalation 12.916 P3 Before inhalation 10.416 1.595 -.370 After inhalation 10.500 P4 Before inhalation 10.409 1.488 -053 After inhalation 10.425

Table 11 shows that the mean value of ASEF indices increased at all electrode attachment sites except for Fp1 (left prefrontal), especially in Fp1 (left prefrontal) (p <0.05) . Such ASEF indicator changes are shown in FIG. 9 as a brain map. It can be seen that the left prefrontal cortex (Fp1) changed from purple to light blue after the inhaling of essential oil.

Therefore, it was confirmed that the emotional comfort of the brain and anxiety decreased after the inhalation of essential oils.

The formulation of the present invention will be described in more detail through the following formulation examples. However, the scope of the present invention is not limited to the formulation example.

Formulation Example 1: Spray formulation perfume composition

95% Ethanol 25.0%

The supernatant-extracted Shin of Production Example 2 at 400 DEG C and 400 bar was mixed with 10.0 wt%

Ion-exchanged water 40.0 wt%

25.0 wt% of liquefied petroleum gas (4 kg / cm 2 20 ° C)

Components other than the liquefied petroleum gas were mixed and stirred to be uniform, and a predetermined amount was put in an aerosol container to mount a valve, and then a liquefied petroleum gas was injected to prepare a spray composition perfume composition.

Formulation Example 2: Gel form fragrance composition

Carrageenan 3.0 wt%

Propylene glycol 2.0 wt%

Propyl paraben 0.3 wt%

The supercritical extraction of the crude supernatant at 400 DEG C and 400 DEG C in Preparation Example 2 at 5.0 wt%

Ion-exchanged water 89.7 wt%

Carrageenan, propylene glycol and propylparaben are mixed, and ion-exchanged water is added while stirring, and this is heated to about 80 캜 while being stirred slowly. Thereafter, the mixture was adjusted to about 65 ° C, and the mixture was stirred at 3000 rpm using a homogenizer. The composition for psychoacoustic perfuming was added to the mixture to form a homogeneous phase. The mixture was poured into a predetermined container and cooled naturally to prepare a gel-type perfume composition.

Formulation Example 3: Liquid Formulation Flavoring composition

95% Ethanol 25.0%

5.0% by weight of a surfactant (polyoxyethylene nonylphenyl ether)

The supercritical extraction of the crude supernatant at 400 DEG C and 400 DEG C in Preparation Example 2 at a concentration of 3.0 wt%

Ion-exchanged water 67.0 wt%

The components other than the ion-exchanged water were mixed, ion-exchanged water was added while stirring slowly, and the mixture was homogenized to prepare a liquid formulation-type perfume composition.

Formulation Example 4: Bath salts

Sodium hydrogencarbonate 70.0 wt%

Anhydrous sodium sulfate 29.0 wt%

The supercritical extraction of the crude supernatant at 400 DEG C and 40 DEG C of Preparation Example 2 in an amount of 1.0 wt%

The gods were stirred until a homogeneous mixture was obtained with a V-type mixer, and then the gods were added with essential oil and sufficiently stirred until further homogeneous.

Formulation Example 5: Skin (lotion)

The supercritical extraction of the crude supernatant at 400 DEG C and 400 DEG C in Preparation Example 2 at a concentration of 3.0 wt%

12.0% by weight of hydroxyethylene cellulose (2% aqueous solution)

Xanthan gum (2% aqueous solution) 2.0 wt%

Butylene glycol 6.0 wt%

Glycerin 4.0 wt%

5.0% by weight sodium hyaluronate (1% aqueous solution)

Ion-exchanged water 73.0 wt%

Lotion was prepared by a conventional method of producing the lotion.

Claims (7)

delete delete delete delete delete delete The perfume composition according to any one of claims 1 to 4, wherein the perfume composition contains a concentrated Magnolia Flos essential oil extracted at 400 bar at 400 bar as an active ingredient and simultaneously enhances the emotional comfort and arousal effect of the brain.

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