KR20200045213A - An aromatherapy mask pack containing natural petals - Google Patents

Abstract

The present invention relates to an aromatherapy mask pack with excellent bio-safety and, more specifically, to an aromatherapy mask pack comprising: a cosmetic composition containing natural petals and essential oils as active ingredients; and a sheet layer.

Description

Aromatherapy mask pack containing natural petals {AN AROMATHERAPY MASK PACK CONTAINING NATURAL PETALS}

The present invention relates to a cosmetic composition and a perfume composition containing natural petals, it can be usefully utilized in a mask pack having an aromatherapy effect.

In general, human skin is susceptible to change with age, and the degree of change is large and varies depending on the area.

Recently, interest in skin beauty has been continuously increasing, and there is an increasing demand for an effective method for preventing changes in skin wrinkles or pigmentation caused by skin aging.

Various types of packs, cosmetics, etc. have been manufactured and used to prevent skin aging, but since ingredients contained in the packs or cosmetics irritate the skin and cause skin diseases, use natural materials that show excellent biocompatibility and non-toxic properties Therefore, it has been used in the manufacture of packs or cosmetics.

Natural ingredients have fewer side effects on the skin, and recently, as consumers' response to cosmetics using natural ingredients has increased, the development value of cosmetics has increased.

Accordingly, the present inventors tried to develop an eco-friendly cosmetic product that can safely use sensitive skin by containing only natural ingredients corresponding to EWG 1 grade.

The present invention is to solve the problems of the prior art described above, the object of the present invention is to provide a natural fragrance and cosmetic composition that helps to improve the skin with a stress relief and soothing effect.

According to an aspect of the present invention, a cosmetic composition comprising natural petals and essential oils as active ingredients; And a sheet layer; an aromatherapy mask pack is provided.

In one embodiment, the natural petals are steamed and compressed on the sheet layer, and the essential oil may be impregnated on the sheet layer.

In one embodiment, the sheet layer may be a natural fiber, a synthetic fiber, a mixed fabric of these, or a non-woven material.

In one embodiment, the natural petals may be selected from one or more of the group consisting of roses, calendula, and lavender.

In one embodiment, the natural petals can be obtained by inoculating fermentation strains.

In one embodiment, the essential oil may be one or more selected from the group consisting of rose oil, calendula oil, and lavender oil.

In one embodiment, the essential oil may further include tea tree leaf oil.

In one embodiment, the mask pack may further include skin soothing, anti-inflammatory or antibacterial activity.

According to another aspect of the invention, (a) the step of steaming and compressing the natural petals on the sheet layer; And (b) impregnating the sheet layer with a cosmetic composition comprising natural petal essential oil. A method of manufacturing an aromatherapy mask pack is provided.

In one embodiment, the natural petals may be selected from one or more of the group consisting of roses, calendula, lavender.

In one embodiment, the essential oil may be one or more selected from the group consisting of rose oil, calendula oil, and lavender oil.

According to the present invention, the aromatherapy mask pack is simultaneously applied with natural petal oil along with natural petals, and thus not only has excellent skin improvement effect but also excellent biosafety.

In particular, the natural raw material applied to the present invention is a raw material corresponding to the EWG 1 grade and can be safely applied to sensitive skin without any harmful effect to the human body.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the present invention.

Hereinafter, the present invention will be described with reference to the accompanying drawings. However, the present invention may be implemented in various different forms, and thus is not limited to the embodiments described herein. In addition, in order to clearly describe the present invention in the drawings, parts irrelevant to the description are omitted, and like reference numerals are assigned to similar parts throughout the specification.

Throughout the specification, when a part is "connected" to another part, this includes not only "directly connected" but also "indirectly connected" with another member in between. . Also, when a part is said to “include” a certain component, this means that other components may be further provided instead of excluding the other component unless otherwise stated.

Hereinafter, embodiments of the present invention will be described in detail.

One aspect of the present invention is a cosmetic composition comprising natural petals and essential oils selected from one or more selected from the group consisting of roses, calendula and lavender as an active ingredient; And sheet layer; provides an aromatherapy mask pack comprising a.

The "Aromatherapy" is a natural therapy aimed at maintaining homeostasis of the human body by restoring the balance of the body and mind by using the scent and medicinal properties of plants. Refers to a series of therapeutic academic systems aimed at promoting and maintaining psychological health and promoting vitality.

Plants that have fragrance and medicinal properties are used for aromatherapy, among which natural essential oils extracted from herbs are mainly used. The aromatherapy has various effects such as relieving stress, improving immunity, increasing the body's healing power, and helping cell regeneration. Therefore, it is often used in everyday life, and recently it is also used as an auxiliary treatment for various diseases, including cancer treatment.

Unlike the conventional eco-friendly cosmetic product using a natural extract, for example, hot water extract, the cosmetic composition can significantly improve the skin improvement effect by simultaneously applying the natural petal essential oil together with the natural petal crushed product.

The natural petals can be obtained by sterilizing and drying the washed petals, then cutting and crushing them to an appropriate size, and the essential oil can be obtained by a method well known in the art, for example, physically compressing the natural petals strongly. By doing so, the oil component contained in the petals can be separated.

In particular, since the natural raw material contains only EWG grade 1 ingredients, it satisfies both functionality and safety, and is not harmful, so it is easy to apply to sensitive skin.

EWG grade is a non-profit environmental civic group in the United States announced by the EWG (Environmental Working Group), which is a harmful component of cosmetic ingredients. Class 2 and Class 2 are classified as safety class.

The “rose” refers to the flower of the rose (Rosa hybrida), which collectively refers to the plant belonging to the family Rosaceae, Rosaceae, and the wild species of the rose is native to West Asia and distributed in one, subtropical, temperate, subtropical regions of the Northern Hemisphere More than 100 are known.

Typically, roses refer to these wild and natural hybrids. A rose is a horticultural species that has been cultivated for ornamental and fragrance purposes because of its beautiful shape and fragrance. Roses were cultivated by wild species in Europe and their natural hybrids in West Asia during the Greek and Roman periods. From this time on, many were cultivated mainly in southern Europe over the Renaissance period.

The rose oil squeezed the rose contains rich vitamin C. In Europe, wild rose buds have been used as a raw material for lotion, or used to treat wounds and burns. In particular, the bath with rose buds not only suppresses skin inflammation, but also enhances cell vitality, resulting in shiny skin. It has a nourishing effect.

In addition, natural petals contain oil-soluble essential oils beneficial to the skin and water-soluble ingredients such as vitamin C, tannin, and citric acid to give vitality to tired skin, and soft texture makes natural cellulose petal massage without irritation Effects can also be given.

Above “ Calendula officinalis ) ”is a dicotyledonous perennial plant of asteraceae, a marigold or calendula in the East, and also called Marigold in the West, native to Southern Europe.

In oriental medicine, the properties of calendula are dissolving small gallstones, acting to send out large gallstones, and are described as being effective against various cancers. In the West, petals are used as a dye to drink salads, soups, tea, and yellow.

The “Lavender” is a dicotyledonous plant, a perennial herb belonging to the genus Lamiaceae and Lavandula. Officinalis ) with purple or purple flowers, and has a refreshing and light herb scent throughout the aeration. Lavender oil obtained by extracting lavender outpost or by steam distillation is known to activate cells in the skin, act as an insecticide and disinfectant, and is effective in relieving fatigue, reducing tension, and insomnia.

The natural petals can be obtained by inoculating fermentation strains. The natural petals have a smaller particle size of useful components and a more toxic decomposition by the fermentation process, and thus the skin absorption rate can be improved, and skin troubles or allergic side effects can be alleviated.

The natural petals may be prepared by drying naturally or using a device, specifically, a rotary pressure concentrator and a freeze dryer, followed by inoculation of a fermentation strain and fermentation for a period of time under predetermined conditions.

The fermentation strain is Lactobacillus sp . , Monascus sp . , Bifidobacterium sp . ), Prevotella sp . , Fusobacterium sp . ), And one or more selected from the group consisting of Eubacterium sp . Strain, but preferably may be a fermented extract obtained by inoculating a Lactobacillus sp . Strain.

The essential oil may be one or more selected from the group consisting of rose oil, calendula oil, and lavender oil.

The cosmetic composition may implement a remarkably superior skin improvement activity than when applying a natural extract or oil alone by applying the essential oil together with the natural petals.

In addition, the cosmetic composition may further include tea tree leaf oil.

Above “Tea Tree ( Melaleuca alternifolia ) ”belongs to the genus Leptospermum, Melaleuca, and Kunzea. There are about 86 plants in the genus Leptospermum (genus) and about 230 plants in the Melaleuca. The origin of the tea tree is Australia, which grows in New South Wales, grows in swamps up to about 6 m in height, and has strong vitality, so it can grow well even after cutting stems.

The tea tree contains Terpinen-4-ol, g-terpinene, 1,8-cineol, etc., and its efficacy is useful for oily and acne skin due to its converging, cleansing and purifying effect, and in particular, acne with strong antibacterial and antiseptic effect Effective for improvement and prevention.

The cosmetic composition may have skin soothing, anti-inflammatory or antibacterial activity.

The “skin soothing” refers to relieving and subduing erythema or irritated skin areas. For example, the skin soothing may include improving or treating inflammation, reducing percutaneous moisture loss, or reducing redness, but is not particularly limited thereto.

The “anti-inflammatory” refers to the action of inhibiting inflammation, and regulation of the inflammatory response is known to be very complex, which reduces the enhancement and damage of the in vivo repair system.

If the inflammatory response persists due to repeated tissue damage or regeneration, excessive ROS and RNS are generated in the inflammation-related cells and permanent genetic modification may occur. In other words, ROS and RNS are closely related to the inflammatory response that regulates the action of various cells in vivo.

The cosmetic composition is a group consisting of softening lotion, nutrient makeup, moisture cream, nutrition cream, massage cream, nutrition lotion, essence, ampoule, gel, eye cream, oil, foundation, cleansing cream, cleansing foam, cleansing water, spray and powder It may be formulated with one or more selected from, but is not limited thereto.

The cosmetic composition may be formulated by a conventional method. Reference may be made to Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA, in the formulation of external preparations for skin, International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association) in the formulation of cosmetic compositions , Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared as a general emulsifying formulation and a solubilizing formulation. For example, lotion such as flexible lotion or nutrition lotion; Emulsions such as facial lotions and body lotions; Creams such as nutrition cream, moisture cream, and eye cream; essence; Cosmetic ointment; spray; Gel; pack; Sunscreen; Makeup base; Liquid type, solid type or spray type foundation; powder; Makeup removers such as cleansing cream, cleansing lotion, and cleansing oil; Or it may be formulated as a cleaning agent such as cleansing foam, soap, body wash, but is not limited thereto. In addition, the external preparation for skin may be formulated as an ointment, patch, gel, cream or spray, but is not limited thereto.

The cosmetic composition may be appropriately blended with other ingredients within a range that does not impair the purpose according to the present invention, depending on the type of formulation or purpose of use, in addition to the essential ingredients in each formulation.

The cosmetic composition may include an acceptable carrier, for example, oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc. may be appropriately blended. no.

The allowable carrier can vary depending on the formulation. For example, when formulated as ointments, pastes, creams or gels, animal oils, vegetable oils, waxes, paraffins, starches, tracants, cellulose derivatives, polyethylene glycols, silicones, bentonite, silica, talc, zinc oxide or their Mixtures can be used.

When the cosmetic composition is formulated as a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component, and in the case of a spray, chlorofluorohydro It may further include a propellant such as carbon, propane, butane or dimethyl ether.

When formulated as a solution or emulsion, the cosmetic composition may be a solvent, solubilizer, or emulsifier as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-Btylglycol oil can be used, in particular cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.

When formulated as a suspension, the cosmetic composition is a liquid diluent such as water, ethanol or propylene glycol as a carrier component, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or trakant, etc. can be used.

When the cosmetic composition is formulated with soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars, etc. Can be used.

The cosmetic composition according to the quality or function of the final product, fatty substances, organic solvents, solubilizers, thickening agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances commonly used in the industry , Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion blockers, chelating agents, preservatives, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, commonly used in cosmetics It may further contain adjuvants commonly used in the cosmetic or dermatological field, such as any other ingredient.

However, the adjuvant and its mixing ratio can be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.

Another aspect of the present invention is one or more natural petals selected from the group consisting of roses, calendula, lavender; And essential oil; provides a fragrance composition comprising as an active ingredient.

The perfume composition may include natural oil along with natural petals, and may be a composition for improving mood and depression. The composition can relieve tension, relax, stabilize emotions and relax the mind and body.

The perfume composition may include at least one essential oil selected from the group consisting of rose oil, calendula oil, and lavender oil, and may further include tea tree leaf oil.

The perfume composition may increase alpha-waves during brain waves and decrease beta waves (β).

The “alpha-wave” is a wave having a frequency of 8 to 13 Hz in an EEG, meaning that it comes from the occipital part of the brain cortex. Waves similar to the scurve appear in both sides of the occipital dominance with left and right symmetry. The amplitude repeats some increase or decrease in a period of 1 to several seconds. It occurs when a normal adult relaxes and rests.

The “beta wave” (β) refers to a wave having a frequency of 13 Hz or more in brain waves, and appears in the eyes or mental activities in normal people, and is generally low in amplitude and has little regularity due to symmetry. It occurs at work and can also occur under stress.

The term “stress relief” refers to alleviating and reducing the degree of stress, which is a non-specific bioreaction that occurs in the body against various injuries and stimuli inflicted on a living body, and in particular, to relieve mental stress or tension caused by external factors. it means.

Unlike the physical stability, the “psychological stability” may mean that the mind does not feel comfortable and uneasy from a psychological point of view and maintains a stable state or that the state persists. In addition, the psychological stability may mean a state in which the mind is relaxed away from stress, and it means that the emotion is stabilized and relaxed.

The term “relax” means to relax or psychologically relax and feel relieved or relaxed.

The natural petals and essential oils have a light, refreshing, elegant and refreshing fragrance, and have excellent effects on skin beauty. In addition, the fragrance from the natural petals and essential oils is a kind of plant pheromone, which acts on the brain of the brain through the sense of smell to secrete serotonin. Serotonin is a stress-resisting hormone that helps liberate oppressed emotions and is effective in treating and preventing depression.

When the content variation between each active ingredient constituting the perfume composition is outside the above range, the synergistic effect according to the interaction between the active ingredients may not be properly implemented, and is mixed in consideration of the working environment and the quality of the final product The ratio can be appropriately controlled.

Another aspect of the present invention is a cosmetic composition comprising natural petals and essential oils as an active ingredient; And sheet layer; provides a mask pack comprising a.

The natural petals are steamed and compressed on the top of the sheet layer, and the essential oil may be formulated as a cosmetic composition and impregnated into the sheet layer.

The steaming treatment may be performed using steam heated to 40 to 100 ° C., or may be performed by immersing the natural petals in water heated to the above-mentioned temperature.

The steaming treatment time may be appropriately adjusted according to the temperature, and may be appropriately adjusted according to the type of the natural leaf or the state of the leaf.

The sheet layer may be a natural fiber, synthetic fiber, a mixed fabric of these, or a non-woven material.

The sheet layer is sufficient as long as it is a material that is harmless to the human body and the cosmetic composition can be applied in a large amount, and may be natural fibers, synthetic fibers, mixed fabrics thereof, or non-woven materials, preferably non-woven fabric, pure cotton, spunlace, pulp, Or it may be composed of a polymer water-based gel.

Hereinafter, the present invention will be further described through examples, but it is apparent that the present invention is not limited by the following examples.

Manufacturing example  1: Natural petals / Fermented natural petals

The rose petals, calendula leaves, and lavender leaves were washed and sterilized, respectively, and then dried completely at room temperature to obtain natural petal pulverized products having an average diameter of about 2.0 mm, respectively.

The solid matter of the obtained pulverized was inoculated with Monacus purpureus to be 1000cfu / g, solid fermented for 24 hours, and then steam sterilized at 125 ° C for 30 minutes (high pressure sterilizer, Sanyo).

The obtained fermentation product was dried completely under aseptic conditions to obtain fermented natural petals.

Manufacturing example  2: Natural petal oil

After washing and sterilizing rose petals, calendula leaves, and lavender leaves, natural petal oil was obtained through a compactor. The oil obtained was filtered through a 0.5 μm filter to remove the residue.

Comparative manufacturing example  : Natural petal extract

After washing the rose petals, calendula leaves, and lavender leaves, each was dried at room temperature and pulverized to obtain 100 g of each pulverized product.

Extraction was performed at 80 ° C. for 12 hours using a 10-volume aqueous ethanol solution (70% w / w) as a solvent for 100 g of the pulverized material.

The extract was filtered through a 250 mesh and 0.5 µm filter, and the remaining raw materials were extracted three times in the same manner. The extract was concentrated under reduced pressure at 50 ° C, and freeze-dried to obtain a solid content.

Example  And Comparative example

The samples were mixed and formulated with fragrances or essences as described below to verify the mood change effect or skin improvement effect of the obtained samples.

[Content (parts by weight)] division Example One 2 3 4 5 6 7 8 9 10 Rose petals 30 - - 30 - - 15 - 10 - Calendula leaves - 30 - - 30 - 15 15 10 - Lavender leaves - - 30 - - 30 - 15 10 - Rose oil 60 - - 30 - - 20 - 15 15 Calendula Oil - 60 - - 30 - 20 20 15 15 Lavender oil - - 60 - - 30 - 20 15 15 Tea tree leaf oil - - - 30 30 30 20 20 15 15 Fermented rose petals - - - - - - - - - 10 Fermented calendula leaves - - - - - - - - - 10 Fermented lavender leaves - - - - - - 10

[Content (parts by weight)] division Comparative example One 2 3 4 5 6 7 8 9 Rose petals 90 - - - - - - - - Calendula leaves - 90 - - - - - - - Lavender leaves - - 90 - - - - - - Rose oil - - - 90 - - 22.5 60 15 Calendula Oil - - - - 90 - 22.5 - 15 Lavender oil - - - - - 90 22.5 - 15 Tea tree leaf oil - - - - - - 22.5 - 15 Rose flower hydrothermal extract - - - - - - - 30 10 Calendula hydrothermal extract - - - - - - - - 10 Lavender Hot Water Extract - - - - - 10

Experimental example  1: Skin safety test

To verify the skin safety for the samples of Examples and Comparative Examples, MTT assay experiments were performed on fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT).

After the samples of Examples and Comparative Examples were suspended in purified water for each concentration, cell viability was measured.

Cytotoxicity was performed by modifying Mosmann's method of measuring cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent.

Fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT) were respectively dispensed into 96-well plates at a concentration of 1 × 10 ⁴ cells / well and cultured at 37 ° C., 5% CO ₂ for 48 hours. Did.

The culture medium was removed and the sample was incubated for 48 hours in the medium treated by concentration, and then the medium was removed and washed repeatedly with PBS (Phosphate buffered saline).

MTT was dissolved in PBS at 5 mg / mL, 50 L was added, and cultured at 37 ° C and 5% CO ₂ for 24 hours. Dimethyl sulfoxide (DMSO) was added in 100 L per well, stirred for 10 minutes, and absorbance was measured at 540 nm.

As a result of the measurement, cytotoxicity was not confirmed at all concentrations of each sample. The above results suggest that the sample is not only harmless to the human body but also has excellent human safety.

Experimental example  2: Anti-inflammatory activity test

NF-kB reporter assay was used to measure the inhibitory effect of NF-kB transcription. LPS-induced iNOS, COX-2, IFN-β or TNF-α are expressed by the transcription factor NF-kB.

NF-kB is inactivated in a complex state with IkB in the cytoplasm, and when I-kB-α is phosphorylated by I-kB-αkinase, the bound complex is decomposed and activated with NF-kB, which moves into the nucleus (translocation) do.

Induces the expression of the target gene of NF-kB, causing inflammatory action. TRIF, which acts on inflammatory diseases such as multiple sclerosis, is a TIP-domain having a receptor that induces interferon-β, which is a receptor that responds to the activity of toll-like-receptors (TLLs), and the TLRs are invading microorganisms. It recognizes a specific composition and activates an immune response to an antigen.

The receptor recognizes a well preserved pathogenic pattern and activates a signal to stimulate the release of inflammatory cytokines.

IRF-3 (interferon stimulatory gene factor 3) is a transcription activation factor induced by interferon α / β stimulation, and binds to the interferon stimulation response sequence (ISRE) in the transcription control region of most interferon-inducing genes to activate its transcription.

NF-kB-Luc or TRIF depending on the presence of β-galactosidase (receptor molecules such as β (MyD88 and TRIF) using the PEI method in 12-well plates according to the manufacturer's protocol (Jin et al., 2009) Each plasmid containing 1 μg was transfected into HEK293 (1 × 10 ⁶ cells / ml) cells.

After incubation for 24 hours, LPS (100 ng / ml) and samples of the examples or comparative examples were treated, and after 24 hours, NF-kB activity and IRF-3 activity were measured using luciferase assays. Did.

The luciferase assay was performed using a luciferase assay system (Promega Co., USA) reported in the manufacturer's protocol (Jung et al., 2009) (Table 3).

division NF - kB  activation IRF -3 active Control group (blank group) 1.00 1.00 Example 1 1.58 1.83 Example 2 1.60 1.86 Example 3 1.63 1.89 Example 4 1.51 1.76 Example 5 1.55 1.80 Example 6 1.57 1.82 Example 7 1.43 1.67 Example 8 1.45 1.70 Example 9 1.37 1.61 Example 10 1.32 1.56 Comparative Example 1 2.14 2.46 Comparative Example 2 2.05 2.35 Comparative Example 3 2.18 2.40 Comparative Example 4 2.08 2.26 Comparative Example 5 2.15 2.32 Comparative Example 6 2.16 2.43 Comparative Example 7 2.03 2.25 Comparative Example 8 1.96 2.26 Comparative Example 9 1.85 2.03

 [Fold, Luciferase activity] Referring to Table 3, the activity of NF-kB and the activity of IRF-3 were significantly reduced by the samples of the Examples.

The above results suggest that the sample of the example effectively inhibits NF-kB transcription, thereby inhibiting or alleviating inflammation and preventing and improving inflammatory diseases.

Experimental example  3: Antibacterial activity test

The antibacterial activity of the samples obtained in Examples and Comparative Examples was evaluated. As disclosed sludge bacteria Propionibacterium tumefaciens arc Ness (Propionibacterium acnes KCTC 3314) was used, and a chemical synthetic antibacterial agent, triclosan, was used as a positive control.

Propionibacterium acnes are striking in tryptic soy agar medium containing 5% sheep blood and cultured for 2-3 days to prepare McFaland nephleometer standards 0.5 (1% Barum chloride (0.05 ml) + 1% Sulfuric acid) When the turbidity of (9.95 ml) was equal, the concentration of bacteria was 1x10 ⁸ cells / mL.

After diluting each sample from 0 to 2000 ppm in 100 ml intervals in 2 ml of liquid BHI medium, inoculating 20 μL of 1x10 ⁸ cells / mL of the bacterial solution and incubating for 48 hours.

The minimum concentration at which growth was inhibited was defined as the lowest growth inhibitory concentration (MIC) based on turbidity, and the results are shown in Table 4 below.

division Lowest growth inhibitory concentration MIC (PPM) Example 1 720 Example 2 750 Example 3 810 Example 4 660 Example 5 670 Example 6 710 Example 7 640 Example 8 620 Example 9 590 Example 10 560 Comparative Example 1 1050 Comparative Example 2 1100 Comparative Example 3 960 Comparative Example 4 1020 Comparative Example 5 1110 Comparative Example 6 1040 Comparative Example 7 980 Comparative Example 8 950 Comparative Example 9 960 Triclosan 550

Referring to Table 4, the sample of the Example had an equivalent antibacterial activity compared to the positive control triclosan, and the antibacterial effect was superior to that of the Comparative Example. According to the evaluation results, the anti-acne activity was further improved when fermented natural petals (Example 10) or tea tree leaf oil (Examples 4 to 6) were applied.

Experimental example  4: Skin soothing effect test

The soothing effect of the skin of the cosmetic composition including the samples obtained in Examples and Comparative Examples was evaluated. Each sample was formulated with an essence of the same composition, but only the content of the sample was varied.

Essences of formulated examples and comparative examples were applied to 95 selected females aged 20 to 60 years, and skin soothing effects were evaluated.

For 100 women with similar dermatitis symptoms, the five were divided into 19 groups, and the formulated essence was applied to the entire face twice a day for 12 weeks at a temperature of 24 to 26 ° C and 75% humidity.

At the end of the test, subject subjects were asked to fill out a questionnaire to conduct a subjective efficacy assessment.

In the questionnaire, the symptoms of dermatitis are divided into “taste”, “dryness”, “keratinosis”, “dandruff”, “erythema”, “swelling”, “skin cracking”, and “truth and eczema”. In the case of each symptom, the degree of improvement was evaluated (0 to 10 points) and averaged to evaluate the improvement effect of dermatitis symptoms individually (Table 5).

division Evaluation score Example 1 8.1 Example 2 7.8 Example 3 7.7 Example 4 8.3 Example 5 8.0 Example 6 8.1 Example 7 8.5 Example 8 8.4 Example 9 8.6 Example 10 8.9 Comparative Example 1 6.3 Comparative Example 2 5.9 Comparative Example 3 6.2 Comparative Example 4 4.9 Comparative Example 5 6.1 Comparative Example 6 5.9 Comparative Example 7 6.3 Comparative Example 8 5.3 Comparative Example 9 6.7

Referring to Table 5, the sample of the Example was evaluated as having an excellent effect of improving dermatitis compared to the comparative example, and the skin soothing effect was excellent.

Experimental example  5: relaxation activity test

To objectively evaluate the physiological / psychological effects of the samples of the Examples and Comparative Examples, the samples of the Examples and Comparative Examples were formulated with a fragrance composition, and the EEG of the central nervous system and the average heartbeat interval of the autonomic nervous system (average RR interval) , Physiological signals such as skin electrical resistance were measured as follows.

(1) Subject

Subjects were healthy, women who were in their 20s and 40s who were not currently taking medication and were not blind.

On the day before the experiment, we tried to avoid excessive exercise or excessive drinking, and on the day of the experiment, we refrained from smoking, drinks, drugs, and chewing gum, which could affect the central nervous system and sense of smell.

As an experiment on adult women, it was reported that there was a correlation between the change in sensation of aroma and the mood of a woman's menstrual cycle according to the female sexual cycle.

(2) Experiment conditions

The experiment was conducted in the olfactory laboratory (4.8m × 3m × 2.4m), and the intake and exhaust facilities are installed inside for the purpose of reducing the conformity of the olfactory, maximizing the participants' participation in the experiment and affecting the external environment during the experiment Sound insulation was installed to rule out.

During the experiment, the internal temperature (25 ° C), humidity (40-50%), and illuminance (150-200 Lx) were kept constant. After the experiment on one condition was completed, the subject was rested sufficiently to avoid the effect of acclimatization and fatigue, and the experiment was conducted after ventilating the interior of the laboratory.

(3) Measurement method

Electroencephalography (EGASS) is attached to the frontal, parietal, temporal, and occipital lobes according to the international 10-20 electrode placement method, and GRASS (USA) equipment, MP 100 system (USA), and Acknowledge program (USA) are used. Was measured.

The resistance value between each measurement site was 10 ㏀ or less, and the sampling rate was set to 512 Hz.

The electrocardiogram (ECG: Electrocardiogram, CM5 induction method) was attached to the ECG electrode on the chest, and the galvanic skin response was measured by attaching the sensor to the left middle finger and ring finger. The physiological signal was measured using the MP 100 system and the Acknowledge program, and the sampling rate was set to 512 Hz.

(4) Experiment method

The subject was provided with the incense container at a distance of 1-2 cm below the nose with the eyes closed, and the physiological signal was measured while smelling. In order to exclude the effect of the order on the fragrance, the order of providing the fragrance for each subject was randomly performed.

(5) Analysis method

The experiment analyzed data by measuring a total of 50 female subjects. EEG was analyzed by the power spectrum by the FFT method, and then α / (α + βθδβα + βθδ was obtained according to the frequency band. EEG according to the mapping.

The heart rate change rate (HRV) is obtained by obtaining the average RR interval from the R points detected on the electrocardiogram, and according to the power spectrum analysis by the FFT method, LF (Low frequency: 0.04-0.08 Hz), HF (High frequency: 0.15) -0.4 Hz).

The skin electrical resistance (GSR) was calculated by using the potential difference between the two sensors to determine the change over time and the average amplitude of the waveform.

Each physiological signal was compared and analyzed by normalizing the physiological signal values of each scent condition relative to the unscented condition (Normalized Sensitivity).

The mean and standard deviation of the physiological signal analysis values according to the experimental conditions were obtained. For statistical analysis, SPSS program was used to perform ANOVA analysis and t-test for each direction, and the correlation was verified by Pearson correlation analysis.

(6) Experiment result

The ratio of the relative alpha wave to the relative beta wave was analyzed by measuring the central nervous response according to the fragrance composition by brain waves.

Table 6 shows the results of analyzing the ratio of relative alpha waves after use of the perfume composition.

Alpha waves are brain waves that appear when a person is in a comfortable state. A high ratio means that the subject is comfortable with the scent provided, and the high-scented alpha wave means that the body relaxes and soothes.

Referring to Table 6, the perfume composition of the Example had a higher ratio of alpha waves appearing during sedation / relaxation than the comparative example.

division Measurement result Example 1 0.87 Example 2 0.85 Example 3 0.82 Example 4 0.91 Example 5 0.88 Example 6 0.87 Example 7 0.93 Example 8 0.92 Example 9 0.94 Example 10 0.96 Comparative Example 1 0.70 Comparative Example 2 0.71 Comparative Example 3 0.74 Comparative Example 4 0.69 Comparative Example 5 0.63 Comparative Example 6 0.59 Comparative Example 7 0.62 Comparative Example 8 0.68 Comparative Example 9 0.76

Table 7 is a result of analyzing the ratio of relative beta waves according to the use of the fragrance composition of the present invention. Beta waves are brain waves that appear when anxious or nervous, and a high ratio means that the subject is in a tense or awakened state. Referring to Table 7, the perfume composition of the Examples had a lower ratio of beta waves than the comparative examples.

That is, the perfume composition may have a relaxing effect when applied simultaneously when compared to the case where natural petals or natural petal oils are used alone (comparative example).

division Measurement result Example 1 153 Example 2 156 Example 3 158 Example 4 150 Example 5 151 Example 6 153 Example 7 147 Example 8 145 Example 9 142 Example 10 140 Comparative Example 1 182 Comparative Example 2 184 Comparative Example 3 178 Comparative Example 4 181 Comparative Example 5 179 Comparative Example 6 184 Comparative Example 7 176 Comparative Example 8 175 Comparative Example 9 171

The results of measuring the rate of heart rate change and skin electrical resistance after use of the perfume composition are shown in Tables 8 to 10. The rate of heart rate change refers to the degree of high frequency of the heart rate, and in this experiment, the average heart rate (average RR interval: RRI) It was measured by. During tension, the heart rate increases and the heart rate interval narrows, and when relaxed, the heart rate decreases and the heart rate interval widens.

In other words, increasing the average R-R interval means that the heart is beating slowly and feeling comfortable and stable.

division Measurement result Example 1 0.87 Example 2 0.85 Example 3 0.81 Example 4 0.92 Example 5 0.88 Example 6 0.87 Example 7 0.94 Example 8 0.94 Example 9 0.96 Example 10 0.98 Comparative Example 1 0.65 Comparative Example 2 0.66 Comparative Example 3 0.70 Comparative Example 4 0.64 Comparative Example 5 0.56 Comparative Example 6 0.51 Comparative Example 7 0.55 Comparative Example 8 0.62 Comparative Example 9 0.73

Referring to Table 8, the perfume composition of the Example was found to have a wider heart rate interval than the comparative example, and the perfume composition of Example 1 was found to have a wider heart rate interval than Examples 2 and 3. , Compared to when the natural petals or natural petal oil is used alone (Comparative Example), the heart rate interval may be increased when applied simultaneously, especially when the natural extract (Comparative Example 9) is applied. Was not implemented.

Table 9 shows the activity ratio (LF / HF) of the sympathetic nerve to the parasympathetic nerve of the heart after use of the fragrance composition.

The ratio of the activity of the sympathetic nerve to the heart's parasympathetic nerve (LF / HF) is the ratio of the activity of the sympathetic nerve and the parasympathetic nerve, which is proportional to the activity of the sympathetic nerve and inversely proportional to the activity of the sympathetic nerve.

A low sympathetic and parasympathetic activity ratio (LF / HF) of the heart indicates that the activity of the sympathetic nerve is low, which means that the brain is in a stable state.

division Measurement result Example 1 0.17 Example 2 0.18 Example 3 0.19 Example 4 0.16 Example 5 0.16 Example 6 0.17 Example 7 0.15 Example 8 0.15 Example 9 0.14 Example 10 0.14 Comparative Example 1 0.26 Comparative Example 2 0.25 Comparative Example 3 0.24 Comparative Example 4 0.28 Comparative Example 5 0.24 Comparative Example 6 0.27 Comparative Example 7 0.23 Comparative Example 8 0.23 Comparative Example 9 0.22

Referring to Table 9, the fragrance composition of the Example was compared to the comparative example, the activity ratio (LF / HF) of the sympathetic and parasympathetic nerves of the heart was reduced. Table 10 compares the average skin electrical resistance of 1 minute after using the fragrance composition One result. Skin electrical resistance refers to the average amplitude value (potential difference) of changes and waveforms over time, and the tension increases and the amplitude decreases.

division Measurement result Example 1 0.29 Example 2 0.30 Example 3 0.32 Example 4 0.28 Example 5 0.30 Example 6 0.31 Example 7 0.27 Example 8 0.26 Example 9 0.25 Example 10 0.23 Comparative Example 1 0.53 Comparative Example 2 0.54 Comparative Example 3 0.52 Comparative Example 4 0.53 Comparative Example 5 0.52 Comparative Example 6 0.54 Comparative Example 7 0.51 Comparative Example 8 0.51 Comparative Example 9 0.45

Referring to Table 10, the perfume composition of the example effectively reduced the average skin electrical resistance compared to the comparative example, and in particular, when the natural extract (Comparative Example 9) was applied, the same level of relaxation effect as the example was not implemented.

Experimental example  6: Mask Pack  Comparison of skin improvement effect by application

A rayon-PET spunlace (60 gsm) nonwoven layer including viscose rayon and PET (weight ratio 8: 2) was cut into a shape corresponding to the face.

The natural petals steamed on the nonwoven fabric were compressed, and the cosmetic composition containing the essential oil was impregnated.

The soothing effect of the skin was evaluated in the same manner as in Experimental Example 4 using the prepared mask pack.

division Evaluation score Example 1 8.4 Example 2 8.1 Example 3 8.0 Example 4 8.6 Example 5 8.3 Example 6 8.4 Example 7 8.8 Example 8 8.7 Example 9 8.9 Example 10 9.2

As a result of the evaluation, when applied to a mask pack, skin absorption was promoted, and the skin improvement effect was significantly improved.

Experimental example  7: Mask Pack  Comparison of mood change effects by application

A mask pack was manufactured in the same manner as in Experimental Example 6. Using the produced mask pack, the mood change effect was evaluated in the same manner as in Experimental Example 5.

Alpha wave, beta wave, sympathetic nerve to sympathetic nerve activity ratio (LF / HF) and skin electrical resistance were evaluated in order. As a result, when compared to the formulation with essence, when applied in the form of a mask pack, the mood change effect was about 15 to 30 % Improved.

The above results suggest that a better aromatherapy effect can be realized in the form of a mask pack when compared to a simple essence formulation.

The above description of the present invention is for illustration only, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and equivalent concepts should be interpreted to be included in the scope of the present invention.

Claims (11)

Cosmetic composition comprising natural petals and essential oils as active ingredients; And
Aromatherapy mask pack comprising a; sheet layer. According to claim 1,
The natural petals are steamed and compressed on the top of the sheet layer,
Aromatherapy mask pack in which the essential oil is impregnated into the sheet layer. According to claim 1,
The sheet layer is natural fiber, synthetic fiber, a mixture of these, or a non-woven fabric aromatherapy mask pack. According to claim 1,
The natural petals are aromatherapy mask pack selected from one or more of the group consisting of roses, calendula, and lavender. According to claim 1,
The natural petals are aromatherapy mask packs obtained by inoculating fermentation strains. According to claim 1,
The essential oil is an aromatherapy mask pack selected from one or more of the group consisting of rose oil, calendula oil, and lavender oil. The method of claim 5,
The essential oil is an aromatherapy mask pack further comprising tea tree leaf oil. According to claim 1,
Aromatherapy mask pack that further contains skin soothing, anti-inflammatory or antibacterial activity. (a) steaming and compressing natural petals on the sheet layer; And
(b) impregnating the sheet layer with a cosmetic composition comprising natural petal essential oil; a method of manufacturing an aromatherapy mask pack comprising a. The method of claim 9,
The natural petals rose, calendula, lavender, one or more selected from the group consisting of aromatherapy mask pack manufacturing method. The method of claim 9,
The essential oil is a method of manufacturing an aromatherapy mask pack selected from one or more of the group consisting of rose oil, calendula oil, and lavender oil.