WO2011055899A3 - 효율적이고 보편적인 전분화능 줄기세포의 신경세포 분화 유도방법 - Google Patents

효율적이고 보편적인 전분화능 줄기세포의 신경세포 분화 유도방법 Download PDF

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WO2011055899A3
WO2011055899A3 PCT/KR2010/005892 KR2010005892W WO2011055899A3 WO 2011055899 A3 WO2011055899 A3 WO 2011055899A3 KR 2010005892 W KR2010005892 W KR 2010005892W WO 2011055899 A3 WO2011055899 A3 WO 2011055899A3
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cells
differentiation
nerve cells
stem cells
inducing
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WO2011055899A2 (ko
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김동욱
김대성
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연세대학교 산학협력단
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Priority to EP10798707.5A priority Critical patent/EP2502987B1/en
Priority to US13/054,692 priority patent/US8551783B2/en
Priority to ES10798707.5T priority patent/ES2683745T3/es
Priority to JP2011539458A priority patent/JP5756019B2/ja
Publication of WO2011055899A2 publication Critical patent/WO2011055899A2/ko
Publication of WO2011055899A3 publication Critical patent/WO2011055899A3/ko

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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance

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Abstract

본 발명은 줄기세포로부터 신경세포로의 분화 유도방법에 관한 것이다. 보다 구체적으로, 본 발명은 줄기세포의 골 형성 단백질(bone morphogenetic protein) 신호 경로 및 액티빈/노달(Activin/Nodal) 신호 경로를 억제하여 줄기세포로부터 신경세포로의 분화를 유도하는 방법에 관한 것이다. 본 발명은 종래 줄기세포 분화에 사용되었던 부유배양(floating culture) 및 부착배양(attachment culture)등 방법에 관계없이 모든 줄기세포를 효율적으로 신경전구세포로 분화시킬 수 있는 장점이 있다. 또한, 본 발명에 의하여 분화 유도된 신경전구세포는 특정세포(예를 들면, 도파민 신경세포) 또는 올리고덴드로사이트 등으로 고효율로 분화할 수 있으므로, 차후 난치성 신경계 질환(예를 들면, 파킨슨 병이나 척수손상)에 적용할 수 있으며 신약계발에 있어서 기초적인 자료를 제공한다.
PCT/KR2010/005892 2009-11-06 2010-08-31 효율적이고 보편적인 전분화능 줄기세포의 신경세포 분화 유도방법 WO2011055899A2 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP10798707.5A EP2502987B1 (en) 2009-11-06 2010-08-31 Efficient and universal method for inducing the differentiation of nerve cells from pluripotent stem cells
US13/054,692 US8551783B2 (en) 2009-11-06 2010-08-31 Efficient and universal method for neural differentiation of human pluripotent stem cells
ES10798707.5T ES2683745T3 (es) 2009-11-06 2010-08-31 Método eficiente y universal para inducir la diferenciación de células nerviosas a partir de células madre pluripotentes
JP2011539458A JP5756019B2 (ja) 2009-11-06 2010-08-31 効率的で普遍的な多能性幹細胞から神経細胞への分化誘導方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2009-0107235 2009-11-06
KR1020090107235A KR101168053B1 (ko) 2009-11-06 2009-11-06 효율적이고 보편적인 전분화능 줄기세포의 신경세포 분화 유도방법

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Publication Number Publication Date
WO2011055899A2 WO2011055899A2 (ko) 2011-05-12
WO2011055899A3 true WO2011055899A3 (ko) 2011-07-21

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PCT/KR2010/005892 WO2011055899A2 (ko) 2009-11-06 2010-08-31 효율적이고 보편적인 전분화능 줄기세포의 신경세포 분화 유도방법

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US (1) US8551783B2 (ko)
EP (1) EP2502987B1 (ko)
JP (1) JP5756019B2 (ko)
KR (1) KR101168053B1 (ko)
ES (1) ES2683745T3 (ko)
WO (1) WO2011055899A2 (ko)

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Publication number Publication date
EP2502987A4 (en) 2014-04-30
US8551783B2 (en) 2013-10-08
US20110217774A1 (en) 2011-09-08
JP2012501194A (ja) 2012-01-19
ES2683745T3 (es) 2018-09-27
WO2011055899A2 (ko) 2011-05-12
KR20110050310A (ko) 2011-05-13
KR101168053B1 (ko) 2012-07-24
JP5756019B2 (ja) 2015-07-29
EP2502987A2 (en) 2012-09-26
EP2502987B1 (en) 2018-05-16

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