WO2011043584A3 - 형질전환 식물 유래의 고병원성 조류독감 바이러스 단백질 백신 및 그 제조방법 - Google Patents

형질전환 식물 유래의 고병원성 조류독감 바이러스 단백질 백신 및 그 제조방법 Download PDF

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WO2011043584A3
WO2011043584A3 PCT/KR2010/006821 KR2010006821W WO2011043584A3 WO 2011043584 A3 WO2011043584 A3 WO 2011043584A3 KR 2010006821 W KR2010006821 W KR 2010006821W WO 2011043584 A3 WO2011043584 A3 WO 2011043584A3
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transgenic plants
avian influenza
proteins
influenza virus
virus
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WO2011043584A9 (ko
WO2011043584A2 (ko
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황인환
손은주
권은혜
나윤정
전은현
임세진
박기석
성영철
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(주)헬릭스
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Priority to CN2010800563463A priority Critical patent/CN102770016A/zh
Priority to IN3330DEN2012 priority patent/IN2012DN03330A/en
Priority to EP10822229.0A priority patent/EP2486791A4/en
Priority to US13/500,762 priority patent/US20120219580A1/en
Publication of WO2011043584A2 publication Critical patent/WO2011043584A2/ko
Publication of WO2011043584A9 publication Critical patent/WO2011043584A9/ko
Publication of WO2011043584A3 publication Critical patent/WO2011043584A3/ko

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/145—Orthomyxoviridae, e.g. influenza virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein

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  • Genetics & Genomics (AREA)
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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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  • Peptides Or Proteins (AREA)
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Abstract

본 발명은 고병원성 조류독감 바이러스인 H5N1 바이러스의 헤마글루티닌 단백질을 고효율로 발현시키고 항원작용에 필수적인 당화를 위해 식물체에서 발현된 단백질을 소포체로 이동 및 체류시킬 수 있는 식물형질전환용 재조합 벡터를 사용하여 H5N1의 헤마글루티닌을 고효율로 생산할 수 있는 형질전환 식물체의 제조 방법, 상기 형질전환 식물체로부터 항원성 H5N1의 헤마글루티닌을 대량 생산하는 방법 및 상기 형질전환 식물체 또는 상기 식물체로부터 생산된 헤마글루티닌을 포함하는 조류독감 바이러스의 백신조성물에 관한 것이다. 본 발명에 따른 형질전환 식물체를 이용한 H5N1의 헤마글루티닌 생산방법은 저렴한 비용으로 대량 생산이 가능한 효과가 있고, 본 발명에서 사용한 식물형질전환용 재조합 벡터에는 셀룰로오스-결합 도메인이 삽입되어 있어 식물체에서 발현된 단백질을 신속하고 용이하게 분리할 수 있는 효과가 있다. 또한, 본 발명에 따른 형질전환 식물체는 사료 첨가제 등의 형태로 섭취 가능하기 때문에 식용 백신으로 사용할 수 있는 효과가 있으며, 나아가 본 발명에서 생산된 항원성 헤마글루티닌은 동물모델에 투여시 면역 반응을 유도함에 따라 H5N1 조류독감 바이러스에 대한 단백질 백신으로 사용할 수 있는 효과가 있을 뿐만 아니라 조류독감 바이러스의 감염 진단용 시약으로도 사용할 수 있는 효과가 있다.
PCT/KR2010/006821 2009-10-06 2010-10-06 형질전환 식물 유래의 고병원성 조류독감 바이러스 단백질 백신 및 그 제조방법 WO2011043584A2 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CN2010800563463A CN102770016A (zh) 2009-10-06 2010-10-06 来源于转基因植物的高致病性禽流感病毒蛋白疫苗及其制备方法
IN3330DEN2012 IN2012DN03330A (ko) 2009-10-06 2010-10-06
EP10822229.0A EP2486791A4 (en) 2009-10-06 2010-10-06 PROTEIN VACCINE AGAINST HIGHLY PATHOGENIC AVIAN INFLUENZA VIRUS FROM TRANSGENIC PLANTS AND PREPARATION METHOD THEREOF
US13/500,762 US20120219580A1 (en) 2009-10-06 2010-10-06 Highly pathogenic avian influenza virus protein vaccine derived from transgenic plant, and preparing method thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2009-0094627 2009-10-06
KR20090094627 2009-10-06
KR1020100096488A KR101262300B1 (ko) 2009-10-06 2010-10-04 형질전환 식물 유래의 고병원성 조류독감 바이러스 단백질 백신 및 그 제조방법
KR10-2010-0096488 2010-10-04

Publications (3)

Publication Number Publication Date
WO2011043584A2 WO2011043584A2 (ko) 2011-04-14
WO2011043584A9 WO2011043584A9 (ko) 2011-09-01
WO2011043584A3 true WO2011043584A3 (ko) 2011-10-20

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PCT/KR2010/006821 WO2011043584A2 (ko) 2009-10-06 2010-10-06 형질전환 식물 유래의 고병원성 조류독감 바이러스 단백질 백신 및 그 제조방법

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US (1) US20120219580A1 (ko)
EP (1) EP2486791A4 (ko)
KR (1) KR101262300B1 (ko)
CN (1) CN102770016A (ko)
IN (1) IN2012DN03330A (ko)
WO (1) WO2011043584A2 (ko)

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KR101602562B1 (ko) * 2014-06-10 2016-03-10 포항공과대학교 산학협력단 인플루엔자 a 바이러스 h5n1의 헤마글루티닌을 발현하는 식물체 및 사포닌을 포함하는 경구용 인플루엔자 a 바이러스 h5n1 백신 조성물
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Also Published As

Publication number Publication date
EP2486791A4 (en) 2013-06-12
WO2011043584A9 (ko) 2011-09-01
EP2486791A2 (en) 2012-08-15
IN2012DN03330A (ko) 2015-10-23
KR20110037880A (ko) 2011-04-13
US20120219580A1 (en) 2012-08-30
WO2011043584A2 (ko) 2011-04-14
CN102770016A (zh) 2012-11-07
KR101262300B1 (ko) 2013-05-20

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