WO2011021766A2 - Composition for preventing and treating different types of wrinkles depending on physical morphology - Google Patents

Abstract

Provided is a composition for preventing and treating wrinkles, in which individuals' physical morphologies are divided into the bladder-type of human physical morphology and the gastric-type of human physical morphology, and the composition is produced with different constituents and quantities in accordance with each of the human physical morphologies. The composition for preventing and treating wrinkles according to the present invention can provide very much better wrinkle-preventing and softening effects because it is produced with reference to the characteristics of different human physical types, and it is highly stable on the skin and does not give rise to any side effects since the main constituents are natural substances.

Description

Wrinkle prevention and improvement composition according to constitution

The present invention relates to a composition for preventing and improving wrinkles, and more particularly, to divide a person's constitution into a bladder, a constitution, and a stomach and a constitution, and to prepare wrinkles produced by varying the composition and content of the composition according to each constitution. It relates to a composition for prevention and improvement.

In general, theories explaining skin aging include mutagenesis of somatic cells, error generation theory during electronic cloning, gene program theory, free radical theory, autoimmune response theory, toxic substance accumulation theory, and dermal constitutive protein modification theory (M. Rieger, C & T, 110, 94, 1994). Among the theories, free radicals are known to be a major cause of skin aging. As a result of skin aging, wrinkles, skin elasticity, senile spots, etc. occur in the skin appearance, and double wrinkle formation is most representative. Free radicals are chemically highly reactive chemicals that occur when the skin is irradiated with UV light or during the respiration of cells in the skin.

As described above, most of the free radicals related to skin aging are reactive oxygen species (ROS), and examples thereof include superoxide radicals, hydrogen peroxide, hydroxy radicals, and singlet oxygen. These reactive oxygen species are generated during ultraviolet irradiation or respiration process to cause peroxidation of lipids, which are components of cell membranes, through a multi-step chain reaction.

Lipid peroxidation generates various active species such as radicals, peroxides, aldehydes, epoxides, and the like, and damages DNA, RNA, proteins, cell membranes, and cellular structures. Toxicity of these reactive oxygen species is expected to be a major cause of cancer, tissue damage, and aging (Black HS, Photochem photobiol, 46 (2), 213, 1987). Peroxidation of the membrane lipids that make up the cell changes the structure and fluidity of the cell membrane, increases the permeability of cellular material, leaks of lysosome enzymes, inactivates membrane enzymes and carrier proteins, creates covalent crosslinks of lipids and proteins, and It causes chain breakage, DNA damage, and mutations. Among these reactions, peroxidation of dermal cell membranes and modification of dermal constituent proteins are known to be most closely associated with skin aging. However, in spite of many of these studies, it is not yet known how each factor causes skin wrinkles, and thus, research on the mechanism of wrinkle formation is still ongoing.

Materials used to inhibit skin aging to date include enzymes such as superoxide dismutase or catalase; fat-soluble or water-soluble antioxidants such as α-tocopherol (Vitamin E), β-carotene, ascolic acid (Vitamin C), or glutathione, or radical scavenging agents, and the like, which mainly prevent wrinkle formation due to skin aging. It works.

On the other hand, vitamin A, alpha hydroxy acid, and the like, which are recently spotlighted as anti-aging substances, have a healing effect to alleviate wrinkles generated through enhancement of collagen synthesis in the dermis. In particular, vitamin A and alpha hydroxy acids are reported to reduce wrinkles faster than antioxidants and have additional effects such as skin cell regeneration and whitening (R. Hermitte, C & T, 107, 63, 1992). .

Wrinkles are formed as a result of the movement of muscles in a particular direction, which is a result of scaling for a very long time. In addition, wrinkle formation is caused by various factors such as age, external environment, UV irradiation, and the like, and the change that occurs between the dermis and the epidermis by skin aging is related to wrinkle formation.

Until now, AHA and vitamin A (retinoids) are known to be the most effective substances for wrinkle removal, and recently, wrinkle improvement cosmetics that improve wrinkles by controlling the inducing pathways such as collagen triple helix structure change and elastin destruction using peptide ingredients 'S research is active.

AHA and vitamin A are known to penetrate the skin to promote cell production and to increase the synthesis of collagen fibers to remove and reduce wrinkles (Muneo Tanaka. Recent development of skincare products in Japan from the Bio-Science standpoint Fragrance Journal , 9, 1994 p13-23., 光 井 武夫. Aha is mainly contained in natural fruits and milk, such as apples, lemons, grapes, and sugar cane, and examples thereof include organic acids such as lactic acid, glycolic acid, malic acid, tartaric acid, and citric acid, and aha derivatives such as organic acid salts. have. Efficacy of exfoliation, promoting cell regeneration, improving fine wrinkles, increasing moisture retention, and increasing skin elasticity of AHA and its derivatives is already known (MGTucci et al. AHAs and Derivatives Cosmetics & Toiletries., Vol. 113, Mar 1998 pp 55-58).

Vitamin A (retinoid) is also vitamin A and derivatives thereof such as polyethoxylateddretinamide, retinoic acid, retinol, retinyl acetate, retinyl palmitate and the like. The vitamin A is widely known as a substance having the effect of removing wrinkles through cell regeneration and collagen synthesis (Raoul Hermitte.Aged Skin, Retinoids, and Alpha Hydroxy Acids Cosmetics & Toiletries., Vol. 107, Jul. 1992) pp 63-67), and also reports that light aging can be improved (Webb et al. Topical Tretinoin Improves Photoaged Skin, JAMA, 259, vol. 4, Jan, 22/29, 1988, pp95, 527-532). .

Recently, a procedure for removing wrinkles by injecting a substance involved in muscle paralysis called botulinum toxin (Botox) into the wrinkle area has been popularized, and thus, studies on botulinum toxin are being actively conducted (Montecucco, C. and Schiavo). , G. (1994) .Mechanism of action of tetanus and botulinum neurotoxins.Mol. Microbiol. 13, 1-8 Jankovic, J. and Brin, MF (1997) .Botulinum toxin: historical perspective and potential new indications.Muscle Nerve Suppl 6, S129-S145).

In addition, the peptide component is a substance applied to the wrinkle improvement response of botulinum toxin in cosmetics and improves wrinkles through muscle paralysis, thus acting through a wrinkle improvement mechanism different from AHA or vitamin A. In other words, the release of catecholamines (peptide derivative neurotransmitters involved in cell signaling) by interfering with the formation of SNARE (recognition receptors present on the surface of vesicles involved in the transport of substances in cells) complexes involved in neurotransmission pathways By inhibiting the muscles by suppressing the contraction to improve the wrinkles when applied to the skin. European Patent No. 1 180524A1 discloses an amino acid sequence excellent in wrinkle improvement of a peptide, and in particular, it has been found that 6 to 19 peptides among the amino acid sequences exhibit the most wrinkle improvement effect. The peptide currently used as a cosmetic raw material is acetyl hexapeptide-3, the amino acid sequence is acetyl glutamyl- glutamyl- methionyl- glutamyl- arginyl- arginylamine.

Various functional foods and drugs are commercially available for the prevention and improvement of wrinkles as described above, but these functional foods and drugs are uniformly prescribed regardless of the constitution of a person. Therefore, there are many cases in which a drug having excellent effect on a specific constitution is not effective on another constitution.

Therefore, it is necessary to further classify the constitution of a person, and to study and develop the most suitable composition for preventing and improving wrinkles.

The present invention is to solve the problems of the prior art as described above, the object of the present invention is to prevent the wrinkles applied to each of the constitutions so that people of different constitutions effectively prevent and improve the wrinkles according to the constitution It is to provide a composition for improvement.

One aspect of the present invention for achieving the above object is a composition for preventing and improving wrinkles for the bladder and constitution with fat and oily skin, vitamin components including vitamin B, vitamin E and vitamin C; At least one plant extract selected from the group consisting of pomegranate extract, kiwi extract, lemon extract, green tea extract, machi extract and mung bean extract; Adenosine; And it provides a composition for preventing and improving wrinkles comprising acetyl hexapeptide-3.

A second aspect of the present invention for achieving the above object is a composition for preventing and improving wrinkles for the stomach and constitution with dry and dry skin, apple extract, grapefruit seed extract and grapefruit extract in the composition for the bladder and constitution Further comprising one or more of the components, provides a composition for preventing and improving wrinkles.

The composition for preventing and improving wrinkles according to the present invention is applied based on two kinds of constitutions classified by a simple method, and more effectively prevents and improves wrinkles as compared to the composition for preventing and improving conventional wrinkles. Since it is the active ingredient, there are no side effects due to the application.

As a result of continuous observation after applying the composition for preventing and improving wrinkles according to the present invention to each of the two groups of constitutions, the wrinkle improvement of almost all the subjects was confirmed, and no side effects occurred.

Hereinafter, the present invention will be described in more detail.

According to the present invention, the composition for preventing and improving wrinkles may be classified into two types according to the relative activity of organs, and may provide a composition for preventing and improving wrinkles suitable for each constitution.

Organs of the human body can be largely classified into organs located on the left (upper abdomen) centered on the stomach and on the right (lower abdomen) centered on the bladder. Depending on the person's individual constitution, the activities of the organs located on the left (above) are more active than those of the organs located on the right (lower), and vice versa. In the present invention, the former belong to the stomach and constitution, and the latter belong to the bladder and constitution. According to this definition, people were divided into two constitution groups, and according to the constitution groups, the composition for preventing and improving wrinkles, respectively, was developed.

Bladder and constitution are usually fat people. This type of constitution is for people whose organs located on the right side of the body (lower belly) are relatively weaker than gastrointestinal activity, have slow metabolic functions, and have mostly oily skin.

In contrast, the stomach and constitution is generally a person with a thin body. This constitution is a person whose stomach activity is relatively weak compared to the activities of the organs located on the right side (bottom belly), but the body metabolism is active, so it is difficult to gain weight even if you eat a lot, mainly people with dry skin.

One aspect of the present invention, as a composition for preventing and improving wrinkles for bladder and constitution according to the constitution classification, vitamin component including vitamin B, vitamin E and vitamin C; At least one plant extract selected from the group consisting of pomegranate extract, kiwi extract, lemon extract, green tea extract, machi extract and mung bean extract; Adenosine; And it relates to a composition for preventing and improving wrinkles comprising acetyl hexapeptide-3.

In the composition, the vitamin component is vitamin A, including retinol, beta carotene, tretinoin, alpha carotene and the like; Vitamin B, including thiamine, riboflavin, nicotinic acid, pantothenic acid, panthenol, panthecin, pyridoxine, biotin, folic acid, foline acid, cyanocobalamin, niacinamide and the like; Vitamin C, including ascorbic acid, dihydroascorbic acid, and the like; Vitamin D, including ergosterol, ergocalciferol, cholecalciferol, calcitriol and the like; Vitamin E, including tocopherol, tocopherol acetate, tocotrienol, and the like; Vitamin K, including naphthoquinone, phylloquinone, menaterenone, menadione, and the like, of which vitamin B, vitamin E and vitamin C are preferred. Among these, it is particularly preferable to use at least one vitamin among panthenol, niacinamide, tocopherol acetate and vitamin C.

The content of the vitamin component is 0.05 to 3% by weight is appropriate in terms of the wrinkle improvement effect. Particularly preferably, panthenol is 0.01 to 1% by weight, niacinamide is 0.01 to 0.1% by weight, tocopherol acetate is 0.01 to 0.1% by weight, and vitamin C is preferably 0.01 to 1% by weight. If the content of the vitamin component is less than the above range, the wrinkle improvement effect is not sufficient, and if it exceeds the above range, there is no apparent increase in effect compared to the increase in content.

In the composition, the plant extract means various substances obtained from each part of the plant. The plant extract applicable to the composition for improving wrinkles and preventing wrinkles according to the present invention, it is preferable to use at least one of pomegranate extract, kiwi extract, lemon extract, green tea extract, machi extract, mung bean extract.

The content of the plant extract is 1 to 13% by weight is appropriate in terms of the wrinkle improvement effect. Particularly preferably, the pomegranate extract is 0.1 to 2% by weight, the kiwi extract is 0.1 to 2% by weight, the lemon extract is 0.5 to 3% by weight, the green tea extract is 0.1 to 2% by weight, the gusset extract is 0.1 to 2% by weight, Mung bean extract is preferably 0.1 to 2% by weight. If the content of the plant extract is less than the above range, the wrinkle improvement effect is not sufficient, and if it exceeds the above range, there is no apparent increase in effect compared to the increase in content.

The plant extract included in the composition of the present invention may extract each plant using water, an organic solvent, or a mixed solvent thereof. All of these components may be mixed and extracted at the same time, or individual components may be extracted and mixed under optimum conditions. In the case of hot water extraction, for example, the components of the composition of the present invention are added to water and heated at a temperature of at least 70 ° C, preferably at least 80 ° C, more preferably at least 90 ° C, for at least 2 hours, preferably at least 3 hours, The heated mixture can be prepared by filtration. When heating at a high temperature, the time is reduced, but the active ingredient is destroyed and can be lost. When heating at a temperature lower than the temperature, it is important to control the temperature and time properly because the active ingredient of the mixture is not completely extracted. This extract can be dried or used as it is. When extracted using an organic solvent, alcohols such as methanol, ethanol, isoprotanol, butanol, ethylene, acetone, ether, hexane, chloroform, ethyl acetate, DMF (N, N-dimethyl formamide), DMSO (dimethyl sulfoxide) Side), mixtures thereof and the like can be extracted with an organic solvent at room temperature or by heating.

As one specific example, the plant is collected and pulverized and refluxed with 5 to 20 volumes of water or 1 to 4 carbon atoms, anhydrous or hydrous lower alcohol, ethyl acetate, acetone or hexane based on the dry weight of the mill. The extract may be extracted by heating at 40-100 ° C. for 1 to 5 hours or by immersion at room temperature for 1 to 30 days.

The extract extracted by heat extraction or cold extraction is filtered to remove suspended solid particles, for example, by filtering the particles using nylon or the like, or filtering using a freeze filtration method, and then using them as they are or using lyophilization, vacuum drying, and hot air. Drying, spray drying, etc. may be used for drying.

In the composition, the adenosine is a nucleoside in which adenine is connected to beta-N9-glycosidic bonds to a part of ribose (ribofuranose), and mainly contributes to biochemical action in the form of adenosine diphosphate or triphosphate. The adenosine content is appropriately 0.02 to 0.07% by weight, more preferably 0.04 to 0.06% by weight in view of the wrinkle improvement effect. If the content of adenosine is less than the above range, the effect of improving wrinkles is not sufficient, and if it exceeds the above range, there is no apparent increase in effect compared to the increase in content.

In the composition, the acetyl hexapeptide-3 may act to improve wrinkles through muscle paralysis. The content of acetyl hexapeptide-3 is preferably 0.5 to 4% by weight, more preferably 0.8 to 1.5% by weight based on 100% by weight of the composition. If the content of acetyl hexapeptide-3 is less than the above range, the effect of improving wrinkles is not sufficient, and if it exceeds the above range, there is no apparent increase in effect compared to the increase in content.

The composition for preventing and improving wrinkles according to the present invention is purified water, oil, surfactants, moisturizers, lower alcohols, polyhydric alcohols, thickeners, chelating agents, pigments, preservatives and the like commonly used in general wrinkle improvement and prevention compositions as necessary. It may further comprise one or more components selected from the group consisting of fragrances. Specifically, polyhydric alcohols including glycerin, butylene glycol, erythritol, etc., PEG, PPG, PEG / PPG copolymer, bis PEG-18 methylethyldimethyl silane, dimethicone / vinyl dimethicone cross polymer, polyisobutin ( polyisobutene), polysorbate 20, and the like may further include polymers.

The second aspect of the present invention is a composition for preventing and improving wrinkles for the stomach and constitution according to the above classification, one of the apple extract, grapefruit seed extract and grapefruit extract in the composition for preventing and improving the bladder and constitution It relates to a composition further comprising the above components.

Stomach and sieving is mainly compared to the bladder and sieving because the skin is dry, when adding one or more components of the apple extract, grapefruit seed extract and grapefruit extract can provide the effect of maintaining the moisture of the skin.

The content of one or more components of the apple extract, grapefruit seed extract and grapefruit extract is preferably 0.1 to 3% by weight in terms of the effect of preventing and improving wrinkles, apple extract is 0.01 to 2.0% by weight, grapefruit seed extract is 0.01 to 1.0 More preferably, 0.01% to 1.0% by weight of grapefruit extract is included. If the content of the component is less than the above range, the wrinkle improvement effect is insufficient and it is difficult to give sufficient moisturizing. If the content exceeds the above range, there is no apparent increase in effect compared to the increase in content.

Hereinafter, preferred examples will be described to aid in understanding the present invention. The following examples are merely to illustrate the invention, but are not intended to limit the scope of the invention.

<Production example>

Preparation Examples 1 to 12

To prepare a composition for improving and preventing wrinkles as described in Table 1 and Table 2.

Table 1 (Unit: weight%)

No	Raw material name	Preparation Example 1	Preparation Example 2	Preparation Example 3	Preparation Example 4	Preparation Example 5	Preparation Example 6
One	Purified water	Remaining amount	Remaining amount	Remaining amount	Remaining amount	Remaining amount	Remaining amount
2	glycerin	4.00	6.00	4.00	2.00	3.00	5.00
3	Butylene Glycol	4.00	2.00	2.00	4.00	5.00	3.00
4	PEG / PPG17 / 6 copolymer	1.00	3.00	4.00	4.00	2.00	2.00
5	Adenosine	0.04	0.02	0.03	0.04	0.03	0.049
6	Erythritol	0.50	0.50	0.50	0.50	0.50	0.50
7	Panthenol	0.50	0.50	0.50	0.50	0.50	0.50
8	Niacinamide	0.05	0.05	0.05	0.05	0.05	0.05
9	NATO GUM	0.30	0.30	0.30	0.30	0.30	0.30
10	Citric acid	0.05	0.05	0.05	0.05	0.05	0.05
11	Lactic acid	0.05	0.05	0.05	0.05	0.05	0.05
12	Petlen Glycol	2.00	2.00	2.00	2.00	2.00	2.00
13	Bis PEG 18 ether D	1.20	1.20	1.20	1.20	1.20	1.20
14	V / E Tocopherol	0.05	0.02	0.03	0.07	0.06	0.05
15	TW20	0.10	0.10	0.10	0.10	0.10	0.10
16	Ascorbic acid (vitamin C)	0.01	0.01	0.01	0.01	0.01	0.01
17	Green tea extract	1.00	1.00	1.00	1.00	1.00	1.00
18	Pomegranate extract	1.00	1.00	1.00	1.00	1.00	1.00
19	Kiwi Extract	1.00	1.00	1.00	1.00	1.00	1.00
20	Lemon extract	1.50	1.50	1.50	1.50	1.50	1.50
21	Mung Bean Extract	1.00	1.00	1.00	1.00	1.00	1.00
22	Machi Prefecture Extract	1.00	1.00	1.00	1.00	1.00	1.00
23	Apple extract	1.40	0.20	0.20	1.50	1.20	1.20
24	Grapefruit Seed Extract	0.30	1.30	0.30	0.30	0.30	0.30
25	Grapefruit extract	0.30	0.50	1.50	0.20	0.50	0.50
26	Acetyl hexapeptide	2.00	1.00	3.00	4.00	1.00	1.00
27	Zinc oxide	0.20	0.20	0.20	0.20	0.20	0.20
28	Chitosan	0.05	0.05	0.05	0.05	0.05	0.05
29	Sodium Hyaluronate	2.00	2.00	2.00	2.00	2.00	2.00
30	Beta glucan	1.50	1.50	1.50	1.50	1.50	1.50
31	Sodium Polyacrylate	1.80	1.80	1.80	1.80	1.80	1.80
32	Polyisobutene	0.30	0.30	0.30	0.30	0.30	0.30
33	Dimethicone / Vinyl	1.00	1.00	1.00	1.00	1.00	1.00
Amount		100	100	100	100	100	100

TABLE 2 (Unit: weight%)

No	Raw material name	Preparation Example 7	Preparation Example 8	Preparation Example 9	Preparation Example 10	Preparation Example 11	Preparation Example 12
One	Purified water	Remaining amount	Remaining amount	Remaining amount	Remaining amount	Remaining amount	Remaining amount
2	glycerin	3.00	6.00	4.00	2.00	3.00	5.00
3	Butylene Glycol	6.00	2.00	2.00	3.00	5.00	3.00
4	PEG / PPG17 / 6 copolymer	1.00	3.00	4.00	5.00	3.00	2.00
5	Adenosine	0.04	0.02	0.03	0.04	0.038	0.049
6	Erythritol	0.50	0.50	0.50	0.50	0.50	0.50
7	Panthenol	0.50	0.50	0.50	0.50	0.50	0.50
8	Niacinamide	0.05	0.05	0.05	0.05	0.05	0.05
9	NATO GUM	0.30	0.30	0.30	0.30	0.30	0.60
10	Citric acid	0.05	0.05	0.05	0.05	0.05	0.05
11	Lactic acid	0.05	0.05	0.05	0.05	0.05	0.05
12	Petlen Glycol	2.00	2.00	2.00	2.00	2.00	2.00
13	Bis PEG 18 ether D	1.20	1.20	1.20	1.20	1.20	1.20
14	V / E Tocopherol	0.05	0.02	0.03	0.07	0.06	0.05
15	TW20	0.10	0.10	0.10	0.10	0.10	0.10
16	Ascorbic acid (vitamin C)	0.01	0.01	0.01	0.01	0.01	0.05
17	Green tea extract	1.00	1.00	1.00	1.00	1.00	1.00
18	Pomegranate extract	1.00	1.00	1.00	1.00	1.00	1.00
19	Kiwi Extract	1.00	1.00	1.00	1.00	1.00	1.00
20	Lemon extract	1.50	1.50	1.50	1.50	1.50	1.50
21	Mung Bean Extract	1.00	1.00	1.00	1.00	1.00	1.00
22	Machi Prefecture Extract	1.00	1.00	1.00	1.00	1.00	1.00
23	Apple extract	1.00	0.50	0.20	0	0	0
24	Grapefruit Seed Extract	0.30	0.20	0.10	0	0	0
25	Grapefruit extract	0.30	0.20	0.10	0	0	0
26	Acetyl hexapeptide	0.80	0.90	0.60	0.70	0.50	1.00
27	Zinc oxide	0.20	0.20	0.20	0.20	0.20	0.20
28	Chitosan	0.05	0.05	0.05	0.05	0.05	0.05
29	Sodium Hyaluronate	2.00	2.00	2.00	2.00	2.00	2.00
30	Beta glucan	1.50	1.50	1.50	1.50	1.50	1.50
31	Sodium Polyacrylate	1.80	1.80	1.80	1.80	1.80	1.80
32	Polyisobutene	0.30	0.30	0.30	0.30	0.30	0.30
33	Dimethicone / Vinyl	1.00	1.00	1.00	1.00	1.00	1.00
Amount		100	100	100	100	100	100

Classification of subjects by constitution

(1) Selection of bladder and constitution: It is generally fat, wide shoulders, has a quick nature, weak bladder, easy to get sick, and organs located on the right side of the body (lower belly) than the activity of the stomach The bladder and constitution were selected as a person who has relatively weak, slow metabolic function and is easy to gain weight by eating a little.

(2) Selection of stomach and constitution: It is generally thin and tends to be ill due to weak stomach, and gastrointestinal activity is relatively weak compared to the activities of the organs located on the right side (bottom belly), but the body metabolism function is active. A person with a constitution that is difficult to gain weight was selected as a stomach and constitution.

As a result of classifying volunteers, 62.5% were classified as bladder and constitution, and the remaining 37.5% were classified as stomach and constitution. Among them, 240 applicants were selected for each constitution.

Example 1-1

20 of the bladder and constitution that the wrinkles of the face are visually confirmed, the composition according to Preparation Example 1 was applied to test the wrinkle improvement effect.

Each test subject was tested for 60 days by spreading about 3-4 g of the composition evenly on the face twice daily in the morning and evening. After 60 days of testing, the extent of wrinkle improvement was assessed using the observer's naked eye and questionnaire.

In the evaluation, the test subject was performed without knowing the kind of the composition applied to the subject. The degree of wrinkle improvement was evaluated by marking with ×, Δ, ○, ◎ as shown in Table 3 below, the results of the evaluation of the bladder and the constitution phosphorus is shown in Table 4 below.

Example 1-1 to Example 1-12

Except that the composition according to Preparation Examples 2 to 12 was applied to each of the 20 people with different bladder and constitution was tested in the same manner as in Example 1-1 and the results are shown in Table 4 below.

Example 2-1 to Example 2-12

The test was performed in the same manner as in Example 1-1 except that the composition according to Preparation Example 1 to Preparation Example 12 was applied to each of 20 people having different stomachs and constitutions, and the results are shown in Table 5 below.

TABLE 3

Wrinkle improvement	Visual evaluation of the observer	Survey Evaluation by Subject
×	No wrinkle improvement	No wrinkle improvement
△	Wrinkle improvement was made, but there is no big difference with conventional wrinkle improvement products.	Wrinkle improvement was achieved, but there is no big difference from using the conventional wrinkle improvement product.
○	Further improvement compared to conventional wrinkle improvement products.	It is more improved than when using the conventional wrinkle improvement product, but it is not satisfactory.
◎		It is more improved than when using the conventional wrinkle improvement products, and the psychological satisfaction is also high.

Table 4 Results of evaluation of bladder and constitution

Wrinkle improvement	Example 1-1		Example 1-2		Example 1-3
	Visual evaluation	Survey evaluation	Visual evaluation	Survey evaluation	Visual evaluation	Survey evaluation
×	3	7	5	8	4	6
△	13	12	12	10	12	12
○	4	One	3	2	4	2
◎		0		0		0
Wrinkle improvement	Example 1-4		Example 1-5		Example 1-6
	Visual evaluation	Survey evaluation	Visual evaluation	Survey evaluation	Visual evaluation	Survey evaluation
×	3	5	2	3	One	0
△	12	11	9	8	6	7
○	5	4	9	9	13	12
◎		0		0		One
Wrinkle improvement	Example 1-7		Example 1-8		Example 1-9
	Visual evaluation	Survey evaluation	Visual evaluation	Survey evaluation	Visual evaluation	Survey evaluation
×	0	2	0	One	0	One
△	4	5	5	6	4	4
○	16	13	15	12	16	14
◎		0		One		One
Wrinkle improvement	Example 1-10		Example 1-11		Example 1-12
	Visual evaluation	Survey evaluation	Visual evaluation	Survey evaluation	Visual evaluation	Survey evaluation
×	0	One	0	0	0	0
△	4	5	4	2	5	2
○	16	12	16	14	15	11
◎		2		4		7

Table 5 Evaluation result for stomach and constitution

Wrinkle improvement	Example 2-1		Example 2-2		Example 2-3
	Visual evaluation	Survey evaluation	Visual evaluation	Survey evaluation	Visual evaluation	Survey evaluation
×	0	One	0	2	0	One
△	6	5	4	4	4	4
○	14	13	16	14	16	14
◎		One		0		One
Wrinkle improvement	Example 2-4		Example 2-5		Example 2-6
	Visual evaluation	Survey evaluation	Visual evaluation	Survey evaluation	Visual evaluation	Survey evaluation
×	0	One	0	0	0	0
△	4	5	3	3	3	2
○	16	13	17	16	17	15
◎		One		One		3
Wrinkle improvement	Example 2-7		Example 2-8		Example 2-9
	Visual evaluation	Survey evaluation	Visual evaluation	Survey evaluation	Visual evaluation	Survey evaluation
×	4	7	3	8	3	6
△	13	13	14	12	14	13
○	3	0	3	0	3	One
◎		0		0		0
Wrinkle improvement	Example 2-10		Example 2-11		Example 2-12
	Visual evaluation	Survey evaluation	Visual evaluation	Survey evaluation	Visual evaluation	Survey evaluation
×	3	3	One	2	0	One
△	12	14	14	13	8	8
○	5	3	5	5	12	10
◎		0		0		One

According to the evaluation result of Table 4 and Table 5, all of the compositions of Preparation Examples 1 to 12 were found to be generally effective in improving wrinkles. However, the bladder and the constituents showed the most pronounced wrinkle improvement when the composition of Preparation Example 12 was evenly applied to the face, and in the case of the stomach and the constitution, wrinkles were applied evenly when the composition of Preparation Example 6 was evenly applied to the face. The improvement was found to be most pronounced.

Since the composition according to the constitution, despite the use of the wrinkle improvement effect x or △ evaluated the composition of Preparation Example 6 and the composition of Preparation Example 12 in the weight ratio of 30:70 to 70:30 As a result, the wrinkle improvement effect was evaluated as ○ or ◎ in over 80% of the subjects.

Claims (5)

1. Provides different ingredients and contents for those who have fat body and oily skin (hereinafter referred to as "bladder and constitution") and those who have slim body and dry skin (hereinafter referred to as "gastric and constitution"). Wrinkle prevention and improvement composition,

   The composition for the bladder and constitution is a vitamin component including vitamin B, vitamin E and vitamin C; At least one plant extract selected from the group consisting of pomegranate extract, kiwi extract, lemon extract, green tea extract, machi extract and mung bean extract; Adenosine; And acetyl hexapeptide-3, the composition for preventing and improving wrinkles.
2. The composition for preventing and improving wrinkles of claim 1, wherein the composition for constitution of the stomach and sieving further comprises one or more components of apple extract, grapefruit seed extract and grapefruit extract in the composition for bladder and sieving.
3. According to claim 1 or 2, 0.05 to 3% by weight of the vitamin component, 1 to 13% by weight of the plant extract, 0.02 to 0.07% by weight of the adenosine, 0.5 to 4% by weight of the acetyl hexapeptide-3 based on the total amount of the composition Containing%, wrinkle prevention and improvement composition.
4. The method of claim 2, wherein the apple extract, grapefruit seed extract and the amount of one or more components of the grapefruit extract is 0.1 to 3% by weight, wrinkle prevention and improvement composition.
5. The composition of claim 1 or 2, wherein the composition further comprises at least one component selected from the group consisting of purified water, oil, surfactants, moisturizers, lower alcohols, polyhydric alcohols, thickeners, chelating agents, pigments, preservatives and fragrances. That comprises, wrinkle prevention and improvement composition.