KR20040027829A - Nano liposome composition for herbs - Google Patents
Nano liposome composition for herbs Download PDFInfo
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- KR20040027829A KR20040027829A KR1020040016334A KR20040016334A KR20040027829A KR 20040027829 A KR20040027829 A KR 20040027829A KR 1020040016334 A KR1020040016334 A KR 1020040016334A KR 20040016334 A KR20040016334 A KR 20040016334A KR 20040027829 A KR20040027829 A KR 20040027829A
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- KR
- South Korea
- Prior art keywords
- extract
- composition
- lecithin
- total amount
- composition according
- Prior art date
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- 239000002502 liposome Substances 0.000 title claims abstract description 75
- 235000008216 herbs Nutrition 0.000 title 1
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- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims abstract description 32
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B23—MACHINE TOOLS; METAL-WORKING NOT OTHERWISE PROVIDED FOR
- B23D—PLANING; SLOTTING; SHEARING; BROACHING; SAWING; FILING; SCRAPING; LIKE OPERATIONS FOR WORKING METAL BY REMOVING MATERIAL, NOT OTHERWISE PROVIDED FOR
- B23D61/00—Tools for sawing machines or sawing devices; Clamping devices for these tools
- B23D61/02—Circular saw blades
- B23D61/04—Circular saw blades with inserted saw teeth, i.e. the teeth being individually inserted
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B23—MACHINE TOOLS; METAL-WORKING NOT OTHERWISE PROVIDED FOR
- B23D—PLANING; SLOTTING; SHEARING; BROACHING; SAWING; FILING; SCRAPING; LIKE OPERATIONS FOR WORKING METAL BY REMOVING MATERIAL, NOT OTHERWISE PROVIDED FOR
- B23D61/00—Tools for sawing machines or sawing devices; Clamping devices for these tools
- B23D61/02—Circular saw blades
- B23D61/025—Details of saw blade body
Landscapes
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Medicinal Preparation (AREA)
- Cosmetics (AREA)
Abstract
Description
피부를 통한 약물 전달 경로는 경피, 구강 점막, 상피 세포 점막 등 타겟 질병의 위치와 독성 여부 문제에 따라 다양하게 개발이 되어 왔다. 피부 경유 약물 전달 매개체로서 리포좀 시스템이 널리 사용되고 있는데, 일반적으로 리포좀은 여러 종류의 인지질을 핵심 성분으로 막을 형성하며, 수용액상에서는 구형의 구조물을 형성하는데 리포좀 막 내부에 전달 물질이 내포되는 구조를 이루게 된다.Drug delivery routes through the skin have been developed in various ways depending on the location and toxicity of target diseases such as transdermal, oral mucosa, epithelial cell mucosa. The liposome system is widely used as a drug delivery vehicle through the skin. In general, liposomes form membranes with various phospholipids as core components, and spherical structures in aqueous solution form a structure in which a delivery material is contained in the liposome membrane. .
리포좀 막은 극성 머리와 탄화 수소 꼬리로 이루어진 인지질 분자가 내부의수용성 구획과 외부의 수용액 방향으로 극성 머리가 배치된 이중막 구조를 형성한다. 이러한 리포좀의 양극성 성질을 이용하여 리포좀 내부에 특정 물질을 포접시켜줌으로써 리포좀과 피부 각질 세포 간 지질층과의 융화력에 따른 내접된 수용성 생리 활성 물질의 피부 투과능이 촉진되고 따라서 생체 내 타겟 위치까지 약물이 전달될 수 있게 된다.The liposome membrane forms a double membrane structure in which phospholipid molecules consisting of a polar head and a hydrocarbon tail are arranged with a polar head in the direction of an aqueous soluble compartment and an external aqueous solution. By using a bipolar property of the liposomes by encapsulating a specific substance inside the liposomes, the skin permeability of the insoluble water-soluble physiologically active substance according to the compatibility between the liposomes and the lipid layer between the keratinocytes of the skin is promoted, and thus the drug is introduced to the target location in vivo. Can be delivered.
인지질을 계면 활성제로 사용된 리포좀 제형은 피부 각질층 지질의 유동성을 증가시켜 생리 활성 물질의 피부 투과율을 증가시키고[Biochim.Biophys.Acta, 1237(1995) : 176-182] 피부 자극이 적은 장점이 있으나[PSIT Vol.3, No. 12(2000) : 417-425] 인지질만으로 구성된 리포좀은 구조적 불안정성이란 단점을 가지고 있다.Liposomal formulations using phospholipids as surfactants have the advantage of increasing the fluidity of the stratum corneum lipids to increase the skin permeability of bioactive substances [Biochim. Biophys. Acta, 1237 (1995): 176-182]. PSIT Vol. 3, No. 12 (2000): 417-425] Liposomes composed solely of phospholipids have the disadvantage of structural instability.
계면 활성제로 사용되는 인지질에는 대두나 달걀 유래 레시틴이 있는데 불포화 레시틴으로 제조된 리포좀은 지질의 유동성이 좋아 피부 각질 세포 사이의 지질층, 즉 각질 세포 사이의 간극을 쉽게 통과할 수 있지만 리포좀 구조의 경도가 낮아 그 모양이 쉽게 변하고 천연 식물 추출물이 조성물에 함유되거나 외부 산소, 금속 등에 의해 불포화 레시틴의 이중 결합 부위가 쉽게 산화될 수 있으며, 이에 따라 리포좀 구조가 파괴되어 계면 활성력이 급격히 떨어지게 되고 변색, 변취 문제가 발생하게 된다[Biochemistry Vol.42 No.39 (2003) : 11533-11543].Phospholipids used as surfactants include soy or egg-derived lecithin. Liposomes made of unsaturated lecithin have good lipid flowability and can easily pass through the lipid layer between skin keratinocytes, that is, the gap between keratinocytes. Its low shape makes it easy to change its shape, and natural plant extracts can be contained in the composition, or the double bond sites of unsaturated lecithin can be easily oxidized by external oxygen, metals, etc. Problems arise [Biochemistry Vol. 42 No. 39 (2003): 11533-11543].
수화된 포화 레시틴(hydrogenated lecithin)인 경우에는 불포화 레시틴에서 발생하는 인지질 산화가 일어나지 않기 때문에 변색, 변취, 리포좀 제형 불안정성 문제는 없으나, 레시틴의 지방산 사슬 모두가 포화 탄화수소이므로 지방산 사슬의경직도가 높아져 리포좀 제형의 유동성이 떨어지고 이에 따른 피부 투과능과 피부 친화력이 떨어지는 단점이 있다[Biophysics J. Vol.79 No.1 (2000) : 328-339].Hydrated saturated lecithin does not cause discoloration, odor, and liposome formulation instability because phospholipid oxidation does not occur in unsaturated lecithin, but since the fatty acid chains of lecithin are saturated hydrocarbons, the stiffness of the fatty acid chain is increased, thereby liposome formulation Its fluidity is poor and thus skin permeability and skin affinity are inferior [Biophysics J. Vol. 79 No. 1 (2000): 328-339].
이러한 리포좀 구조에 인지질 막 유동성을 부여해 줄 수 있는 물질로서 콜레스테롤, 당단백질, 터핀 계열 화합물에 대한 연구 결과들이 나오고 있는데[J.Control.Rel. 68(2000) : 85-95, Lipids Vol.32 No.7 (1997) : 769-773], 이러한 물질들의 적절한 배합을 통한 리포좀 구조 유연성 확보와 동시에 리포좀 구조 안정성이 확보된다면 다양한 생리 활성 물질 특히 천연 유래 식물성 물질들과 재조합 단백질 등 효능을 위한 피부 투과능과 동시에 유효 성분의 안정성 확보를 동시에 필요로 하는 분야의 산업적 결과물을 만들 수 있을 것으로 전망한다.As a substance that can impart phospholipid membrane fluidity to the liposome structure, studies on cholesterol, glycoprotein, and terpine-based compounds have been published [J. Control. Rel. 68 (2000): 85-95, Lipids Vol. 32 No.7 (1997): 769-773], various bioactive substances, especially natural It is expected to produce industrial results in fields requiring both skin permeability for efficacy such as derived plant materials and recombinant proteins, and at the same time securing stability of active ingredients.
본 발명은 상기한 문제점들을 해소하기 위한 것으로, 레시틴과 세라마이드 기반 리포좀 조성물에 펜타싸이클릭 트리터핀산과 스테롤이 최적 농도로 리포좀 구조에 구배된 조성물을 통해 생리 활성 천연물 성분 포접능을 최적화 시켜 주고, 동시에 생체 저자극 및 피부 투과능이 향상된 조성물을 구현하고자 한다. 아울러 리포좀 제형에 최적화된 항산화 복합 조성물을 통해 레시틴 기반 리포좀 제형의 산화에 따른 변색, 변취 문제를 해결하고자 한다.The present invention is to solve the above problems, the lecithin and ceramide-based liposome composition to optimize the biologically active natural component inclusion capacity through a composition in which the pentacyclic triterpinic acid and sterol gradient to the liposome structure at the optimal concentration, The present invention seeks to realize a composition having improved bio-hypoallergenic and skin permeability. In addition, through the antioxidant complex composition optimized for liposome formulations to solve the discoloration, deodorant problems caused by oxidation of the lecithin-based liposome formulation.
도1은 본 발명에서 따른 나노 리포좀의 전자 현미경으로 관찰된 구조이다.1 is a structure observed by an electron microscope of a nano liposome according to the present invention.
도2는 나노 리포좀 크기에 대한 입도 분석 결과이다.Figure 2 is a particle size analysis of the nano liposome size.
도3은 인터페론 알파가 포접된 나노 리포좀을 이용한 피부 투과 측정 결과이다.Figure 3 is a skin permeation measurement results using nano liposomes embedded with interferon alpha.
도4는 본 발명에 따른 리포좀의 구조 유연성 테스트4 is a structural flexibility test of liposomes according to the present invention
도5은 본 발명에 따른 리포좀의 구조 안정성 테스트5 is a structural stability test of liposomes according to the present invention
이하 본 발명의 구성 및 작용을 실시예를 통해 보다 상세히 기술한다.Hereinafter, the configuration and operation of the present invention will be described in more detail with reference to Examples.
본 발명에 따른 조성물은 리포좀 혼합물, 유화제, 복합 항산화제, 천연 생리 활성 물질 복합체로 구성이 되며, 리포좀 혼합물은 다시 포화 레시틴, 불포화 레시틴, 세라마이드, 펜타싸이클릭 트리터핀산, 스테롤로 구성이 된다.The composition according to the invention consists of a liposome mixture, an emulsifier, a complex antioxidant, a complex of natural bioactive substances, and the liposome mixture again consists of saturated lecithin, unsaturated lecithin, ceramide, pentacyclic triterpinic acid, sterols.
레시틴은 디올레오일 포스파티딜콜린(DOPC), 디스테아로일 포스파티딜콜린(PSPC), 디팔미토일 포스파티딜콜린(DPPC) 등이 사용되고, 펜타싸이클릭 트리터핀산은 우르솔릭산, 우르솔릭산 유도체 및 이성질체 등이다. 사용 가능한 스테롤은 콜레스테롤(cholesterol), 콜레스테릴 아세테이트(cholesteryl acetate), 콜레스테릴 이소스테아레이트(cholesteryl isostearate), 콜레스테릴 마카다미에이트(cholesteryl macadamiate) 등이다.Lecithin is used for dioleoyl phosphatidylcholine (DOPC), distearoyl phosphatidylcholine (PSPC), dipalmitoyl phosphatidylcholine (DPPC), and the like. Pentacyclic triterpinic acid includes ursolic acid, ursolic acid derivatives, and isomers. The sterols that can be used are cholesterol, cholesteryl acetate, cholesteryl isostearate, cholesteryl macadamiate and the like.
필요에 따라서 추가적으로 가해지는 유화제는 고분자 유화제를 사용하는데 알킬기 탄소수가 10-30인 아크릴레이트/알킬아크릴레이트 크로스 폴리머를 사용하고 유화 보조제로는 천연 고분자 화합물인 카르복시메틸셀룰로오스를 사용한다.The emulsifier to be added as needed is a polymer emulsifier, an acrylate / alkyl acrylate cross-polymer having 10-30 alkyl group carbon atoms, and a carboxymethyl cellulose which is a natural polymer compound as an emulsifying aid.
복합 항산화제로는 지용성 항산화 기능의 토코페롤 및 그 유도체, 비타민 C 유도체, 비타민 A 유도체와 L-트립토판을 사용하고 수용성 항산화제로서 소듐 메타포스페이트(Sodium metaphosphate), 소듐 타트레이트(Sodium D-tartrate), 소듐 폴리포스페이트(Sodium polyphosphate), 소듐 싸이트레이트(Sodium citrate), 소듐 하이드로진 피로포스페이트(Sodium hydrogen pyrophosphate), 소듐 글루타메이트(Sodium L-glutamate), 소듐 에리소베이트(Sodium erythorbate)를 사용한다.Complex antioxidants include fat soluble tocopherols and derivatives thereof, vitamin C derivatives, vitamin A derivatives and L-tryptophan.Sodium metaphosphate, sodium d-tartrate, sodium as water-soluble antioxidants Sodium polyphosphate, sodium citrate, sodium hydrogen pyrophosphate, sodium glutamate, sodium erythorbate is used.
천연 생리 활성 물질로는 로즈마리 추출물, 병풀 추출물, 신이화 추출물, 감초 추출물, 황금 추출물, 녹차 추출물, 카모마일 추출물, 버섯 추출물, 콩 추출물, 폴리페놀류, 터핀 계열 물질, 이소플라본류, 파이토에스토로겐, 비타민 류, 기능성 단백질 류에서 선택되어진다.Natural physiologically active substances include rosemary extract, centella extract, xinhua extract, licorice extract, golden extract, green tea extract, chamomile extract, mushroom extract, soybean extract, polyphenols, terpineic substances, isoflavones, phytoestrogens, vitamins, It is selected from the class of functional proteins.
상기 주요 성분들의 혼합 비율은 다음과 같다. 리포좀 구성 성분 중 레시틴과 세라마이드가 차지하는 양은 조성물 총 중량 기준으로 0.2 에서 4.0 중량% 에 달하고 바람직하게는 0.5 에서 3 중량%를 제시한다. 세부적으로 레시틴과 세라마이드의 함량 조성 비율은 0.4:1.5:0.1(불포화 레시틴/포화 레시틴/세라마이드) 중량 %로 한다. 리포좀의 유연성을 가해주는 펜타싸이클릭 트리터핀산과 스테롤 함량은 0.1 에서 1.0 중량%이고 바람직하게는 0.2에서 0.6 중량%가 적절하고, 조성 비율은 1:1(펜타싸이클릭 트리터핀산:스테롤)에서 1:4 중량 %로 한다.The mixing ratio of the main components is as follows. The amounts occupied by lecithin and ceramide in the liposome constituents range from 0.2 to 4.0% by weight and preferably from 0.5 to 3% by weight, based on the total weight of the composition. Specifically, the content composition ratio of lecithin and ceramide is 0.4: 1.5: 0.1 (unsaturated lecithin / saturated lecithin / ceramide) by weight. The pentacyclic triterpenic acid and sterol content that gives the liposome flexibility is 0.1 to 1.0% by weight, preferably 0.2 to 0.6% by weight, and the composition ratio is 1: 1 (pentacyclic triterpinic acid: sterol). It is 1: 4 weight%.
추가적으로 가해지는 고분자 유화제는 실시예에 따라서 0.05에서 0.2 중량 %로 한다. 유상 성분에는 스테아릴 알코올과 세틸 알코올 등의 고급 알코올 류를 0.5에서 10 중량 % 첨가한다. 중화제로는 통상적으로 많이 사용하는 트리에탄올 대신 니트로소아민 생성이 적은 테트라히드록시프로필에틸렌디아민을 실시예에 따라 0.01에서 1 중량 %를 첨가한다.Additional polymeric emulsifiers are added at 0.05 to 0.2% by weight, depending on the embodiment. To the oil phase components, higher alcohols such as stearyl alcohol and cetyl alcohol are added at 0.5 to 10% by weight. As a neutralizing agent, tetrahydroxypropylethylenediamine with less nitrosoamine production is added in an amount of 0.01 to 1 wt% according to the embodiment instead of triethanol which is commonly used.
천연 생리 활성 물질 함유량은 1.0에서 20 중량% 첨가하고 복합 항산화제의 농도는 0.1에서 10 중량%를 사용하게 되고 바람직하게는 0.5에서 5 중량 %를 첨가하게 된다. 복합 항산화제의 상세 조성 비율은 토코페롤 1%, vitamin A palmitate1%, vitamin C palmitate 1%, L-tryptophan 2.4% , Sodium metaphosphate 7.2%, Sodium D-tartrate 9.6%, Sodium polyphosphate 14.1%, Sodium citrate 9.9%, Sodium hydrogen pyrophosphate 13.8%, Sodium L-glutamate 2.5%, Sodium erythorbate 35.9% 이다.The natural bioactive substance content is added from 1.0 to 20% by weight and the concentration of the complex antioxidant is from 0.1 to 10% by weight, preferably from 0.5 to 5% by weight. The specific composition of complex antioxidants was 1% tocopherol, 1% vitamin A palmitate, 1% vitamin C palmitate, 1% L-tryptophan, 7.2% Sodium metaphosphate, 9.6% Sodium D-tartrate, 14.1% Sodium citrate and 9.9% , Sodium hydrogen pyrophosphate 13.8%, Sodium L-glutamate 2.5%, Sodium erythorbate 35.9%.
나노 리포좀 제조 방법은 대두에서 추출된 레시틴 중에서 포스파티틸콜린 함량이 90~95%인 불포화 레시틴(Phospolipon 90G)과 포화 레시틴(Lipoid S100-3)를 각 실시예에 따른 적정 농도로 혼합하고 세라마이드 3, 올레놀릭산(oleanolic acid), 콜레스테롤 마카다미에이트(cholesterol macadamidate) 및 에탄올을 혼합하여 60oC에서 가온 용해 시킨 다음, 70oC로 가열되어 용해되어 있는 수상 성분들을 혼합하여 균질기(homogenizer)로 5,000 rpm에서 10분간 유화한 후, 고압 균질기(Microfluidizer)를 사용하여 1,000 기압에서 5회 상온 재순환 처리한다.Nano liposome manufacturing method is a lecithin extracted from soybeans, unsaturated lecithin (Phospolipon 90G) and saturation lecithin (Lipoid S100-3) having a phosphatitylcholine content of 90 ~ 95% and mixed in the appropriate concentration according to each embodiment and ceramide 3 , Oleanolic acid, cholesterol macadamidate and ethanol are mixed and heated at 60 o C, then heated to 70 o C to mix the dissolved aqueous phase homogenizer. After emulsifying at 5,000 rpm for 10 minutes, a high-temperature homogenizer (Microfluidizer) was used to recirculate 5 times at room temperature at 1,000 atmospheres.
<실시예 1,2,3,4,5,6,7,8,9 및 비교예 1,2> 최적 리포좀 조성물 함량 스크리닝Example 1,2,3,4,5,6,7,8,9 and Comparative Examples 1,2> Optimal Liposome Composition Content Screening
<시험예 1 : 나노 리포좀 물리적 성질 분석>Test Example 1: Analysis of physical properties of nano liposomes
본 발명에 따른 리포좀 구조는 저온에서 리포좀 구조를 고정화 시킨 후 투사 전자 현미경(CEM 902, Germany)을 통해 관찰하였고 그 결과는 표1에 정리를 하였다.The liposome structure according to the present invention was observed through a projection electron microscope (CEM 902, Germany) after fixing the liposome structure at low temperature and the results are summarized in Table 1.
결과에 따르면 본 발명에 따른 리포좀의 평균 크기는 100~200 nm 범위를 갖음을 확인할 수가 있다.According to the results it can be confirmed that the average size of the liposome according to the present invention has a range of 100 ~ 200 nm.
입도 분석기(Laser Light Scattering System)를 통한 나노 리포좀 크기 분포 분석 결과는 도2에 나타나 있고 약 100nm 주변에 분포가 되는 특성을 보여 준다.The results of nano liposome size distribution analysis using a particle light analyzer (Laser Light Scattering System) are shown in FIG. 2 and show characteristics of distribution around 100 nm.
리포좀은 일반적으로 온도가 높아지면 구조의 경도가 커지게 되고 온도가 낮아지면 리포좀 막 유동성이 증가하게 되는데, 이는 리포좀 내 유효 성분의 방출능에 영향을 주게 된다. 본 발명에 따른 리포좀 조성물은 기존 일반적인 리포좀 구조와는 달리 저온이든 고온에서든 리포좀 막의 적정 유동성을 가지게 됨으로써 리포좀 내 유효 성분의 불필요한 방출이나 적정 속도로 방출 속도를 가짐으로써 최적의 생리 활성 전달 구조체 역할을 하게 된다.In general, liposomes have increased structure hardness at higher temperatures and increased fluidity of liposome membranes at lower temperatures, which affects the release of active ingredients in liposomes. Unlike the conventional liposome structure, the liposome composition according to the present invention has a proper fluidity of the liposome membrane at a low temperature or a high temperature, thereby serving as an optimal biologically active delivery structure by having an unnecessary release rate or an appropriate rate of release of the active ingredient in the liposome. do.
구체적인 분석 방법은 형광 표지 물질로서 DPH(1,6-diphenyl-1,3,5-hexatriene)을 리포좀 제조시 첨가하여 형광 전이도(fluorescence polarization : 358nm excitation/430nm emission) 변화를 관찰함으로써 리포좀 구조의 유동성을 평가한다. 그 결과는 도5에 정리하였다.A specific analysis method is the addition of DPH (1,6-diphenyl-1,3,5-hexatriene) as a fluorescent labeling agent in the preparation of liposomes to observe the change in fluorescence polarization (358 nm excitation / 430 nm emission). Evaluate liquidity. The results are summarized in FIG.
<시험예 2 : 나노 리포좀 구조 안정성 테스트><Test Example 2: Nano liposome structure stability test>
본 발명에서 고안된 리포좀 구조 안정성을 테스트하기 위해 리포좀 내에calcein(미국 Biotium 사 판매) 화합물을 포접하여 시간별 리포좀 안에서 방출되는 calcein양을 분석하였다[Liposome Technology 1983]. 제조된 Calcein 함유 리포좀은 42도 항온 상태에서 형광분석기(Perkin-Elmer luminescence spectrophotometer model LS-5 : 482nm excitation, 512nm emission)를 통해 분석하였고 calcein 방출량은 다음과 같은 공식을 적용하였다.In order to test the stability of the liposome structure designed in the present invention, the calcein amount released in the liposome was analyzed by inclusion of a calcein (sold by Biotium, USA) compound in the liposome [Liposome Technology 1983]. The prepared Calcein-containing liposomes were analyzed by a fluorescence spectrometer (Perkin-Elmer luminescence spectrophotometer model LS-5: 482nm excitation, 512nm emission) at 42 ° C.
Calcein leakage(%) = 100 x (F-Fo)/(Ft-Fo)Calcein leakage (%) = 100 x (FF o ) / (F t -F o )
Ft:t시간대 계면 활성제로 리포좀 구조 파괘했을 때 형광 세기F t : t Fluorescence intensity when liposome structure was disturbed with time zone surfactant
Fo:초기 형광 세기F o : Initial fluorescence intensity
F : t시간대 형광 세기F: t-time fluorescence intensity
결과는 도6에 정리가 되었고 비교예 3에 대한 결과에서 알 수 있듯이 약 1시간 가량 후에 80%가량의 방출이 있지만, 본 발명에서 고안된 리포좀 조성물인 실시예 10,11,12,13에 대한 결과는 20% 미만의 안정적인 리포좀 포접능을 보여 준다.The results are summarized in FIG. 6, and as can be seen from the results for Comparative Example 3, there was about 80% of release after about 1 hour, but the results for Examples 10, 11, 12, and 13, which are the liposome compositions designed in the present invention. Shows stable liposome inclusion of less than 20%.
<시험예 3 : 나노 리포좀 제형 안정성 테스트><Test Example 3: Nano liposome formulation stability test>
본 발명에 따른 리포좀의 안정성 테스트를 위해 각 실시예 별 시간에 따른 리포좀 크기 변화를 관찰하였다. 시간에 따라 리포좀 크기는 전반적으로 커지게 되고 크기가 커지면 리포좀 구조 불안정성이 증가되는데, 본 발명에 따른 실시예를통해 리포좀 구조 안정성이 증가됨을 확인할 수가 있다. 그 결과는 표2에 정리되었다.In order to test the stability of liposomes according to the present invention, changes in liposome size with time for each example were observed. The liposome size increases with time and the liposome structure instability increases as the size increases, and it can be seen that the liposome structure stability is increased through the embodiment according to the present invention. The results are summarized in Table 2.
<시험예 4 : 리포좀 제형의 과산화물가 분석>Test Example 4: Peroxide Value Analysis of Liposomal Formulations
레시틴 리포좀 제형의 산화 스트레스에 대한 안정도를 측정하기 위해 과산화물가(peroxide value)를 통해 산패도(酸敗度)를 분석했다. 과산화물가 측정은 통상적인 방법인 요오드 적정법을 이용하였다. 그 결과는 표3과 표4에 나타내었고, 본 발명에 따른 실시예 6,7,8,9,에 첨가된 복합 항산화제에 의해 레시틴 제형이 산화 안정도가 증가함을 알 수 있다.In order to measure the stability against oxidative stress of the lecithin liposome formulation, the acidity was analyzed by the peroxide value. Peroxide value was measured by the usual method of iodine titration. The results are shown in Table 3 and Table 4, it can be seen that the oxidative stability of the lecithin formulation is increased by the complex antioxidant added in Examples 6,7,8,9, according to the present invention.
<시험예 5 : 경피 흡수 테스트>Test Example 5: Percutaneous Absorption Test
본 발명에서 고안된 리포좀 조성물의 경피 흡수능을 평가하기 위해서 tape stripping 방법[Int.J.Pharm. 139(1996) 197-203]을 사용하였고, 구체적인 방법을 기술하자면 약 2mM 에 해당하는 NaFI를 비교예1, 실시예1,2,3,4, 제형에 포접시켜주고 나서 10명의 건강한 성인을 대상으로 팔뚝 상박 부위에 약 30분간 도포한 후, scotch magic 테이프(3M사 판매)로 연속해서 15번 각각 도포 부위에 균일한 압력으로 문지른다. 이렇게 해서 얻어진 각각의 테이프는 5ml 메틴올에 넣어 한시간 가량 방치 후에 5ml borate buffer(pH 9)를 첨가하여 형광을 측정한다(Shimadzu RF-540 spectrophotometer, 25도, 493nm excitation/515nm emission)Tape stripping method [Int. J. Pharm. 139 (1996) 197-203], and the specific method is described in that 10 healthy adults were included after inclusion of NaFI corresponding to about 2 mM in Comparative Example 1, Examples 1, 2, 3, and 4, and formulations. After applying for about 30 minutes to the upper arm forearm, rubbing with scotch magic tape (available from 3M) 15 times in succession to each application site with uniform pressure. Each tape thus obtained was placed in 5 ml methinol for about 1 hour, and then 5 ml borate buffer (pH 9) was added to measure fluorescence (Shimadzu RF-540 spectrophotometer, 25 degrees, 493 nm excitation / 515 nm emission).
결과는 다음 표5에 정리가 되었고 본 발병에 따른 리포좀 제형인 경우에 비교예보다 포접된 NaFI 피부 투과능이 우수한 결과을 확인할 수 있었다.The results are summarized in Table 5 below, and in the case of the liposome formulation according to the present disease, the inclusion of NaFI skin permeability was superior to that of the comparative example.
또 다른 피부 투과능 평가 방법으로 인터페론 알파 단백질을 포접시킨(실시예 5) 리포좀을 무모 마우스(hairless mouse)에 도포한 후, 경피 흡수 정도를 관찰하였고 그 결과는 도3에 나타나 있고, 본 발명에 따른 리포좀 조성물은 고분자 단백질의 피부 투과에도 뛰어나다는 결과를 얻을 수 있었다.Another method for evaluating skin permeability was applied to hairless mice with liposomes embedded with interferon alpha protein (Example 5), and then the degree of transdermal absorption was observed and the results are shown in FIG. The liposome composition according to the present invention was excellent in the skin penetration of the polymer protein.
구체적인 실험 방법을 정리하면 무모 마우스에 실험군(실시예 5)과 대조군(비교예 1)를 0.2ml/cm2가량 도포한 후 24시간 방치한다. 테스트 부위 피부 조직을 절단하여 4% paraformaldehyde로 고정화 시켜 준 후, 인터페론 알파에 대한항체(mouse anti-interferon alpha/FITC-conjugated anti-mouse IgG(Sigma))를 통해 투과된 인터페론을 염색한다.In summary, the experimental group (Example 5) and the control group (Comparative Example 1) 0.2ml / cm to the hairless mouse2After applying Leave for 24 hours. After cutting the tissue at the test site and immobilizing it with 4% paraformaldehyde, the antibody was treated with mouse anti-interferon alpha / FITC-conjugated anti-mouse IgG (Sigma). Permeate interferon permeate.
<시험예 6 : 피부 자극 테스트>Test Example 6: Skin Irritation Test
건강한 성인 남녀 실험자 10명의 팔 하박부에 폐쇄 첩포하여 피부 자극 유무를 판정하였다. 그 결과를 표 4에 나타내었다. 하기 결과에서 알 수 있듯이 본 발병에 따른 리포좀 조성물에 대한 피부 자극은 없는 것으로 나타났다.Closure patch was applied to the lower arm of 10 healthy adult male and female experimenters to determine skin irritation. The results are shown in Table 4. As can be seen from the results below, there was no skin irritation for the liposome composition according to the present onset.
0: 자극 없음 1: 최소 자극 2: 약한 자극(홍반)0: no stimulation 1: minimal stimulation 2: mild stimulation (erythema)
3: 심한 자극(홍반, 부종)3: severe irritation (erythema, edema)
다음 실시예는 본 발명에 따른 산업적으로 이용 가능한 조성물들을 예시한 것이고 이에 한정을 두는 것은 아니다.The following examples illustrate, but are not limited to, the industrially available compositions according to the present invention.
<실시예 15 : 여드름 피부용 조성물 예시><Example 15: Example composition for acne skin>
<실시예 16 : 피부 주름 개선 조성물 예시><Example 16: Skin wrinkle improvement composition example>
<실시예 17 : 피부염 개선 조성물 예시>Example 17 Dermatitis Improvement Composition Example
<실시예 18 : 피부 미백 효능 조성물 예시>Example 18 Example of Skin Whitening Efficacy Composition
<실시예 19 : 항균 보습 효능 조성물 예시><Example 19: Antimicrobial moisturizing efficacy composition example>
<실시예 20 : 탈모 예방 및 양모 효능 조성물 예시>Example 20 Hair Loss Prevention and Wool Efficacy Composition Example
<실시예 21 : 건선 피부 개선 조성물 예시>Example 21 Psoriasis Skin Improvement Composition Example
<실시예 22 : 가슴 탄력 개선 조성물 예시>Example 22 Chest Elasticity Improvement Composition Example
본 발명을 통해 고안된 나노 리포좀 조성물은 생체 친화력과 피부 투과능이 뛰어나고 여러 종류의 생리 활성 기능 천연물에 대한 포접능을 최적화할 수 있는 장점을 가지고 있어서 다양한 피부 질환 개선 및 경피 경유 생리 활성 조성물 개발에 사용될 수 있다.The nano liposome composition devised through the present invention has excellent biocompatibility and skin permeability, and has the advantage of optimizing the inclusion ability for various kinds of physiologically active natural products, and thus can be used for improving various skin diseases and developing transdermal bioactive compositions. have.
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