WO2011009015A1 - (+) - morphinans as antagonists of toll-like receptor 9 and therapeutic uses thereof - Google Patents

(+) - morphinans as antagonists of toll-like receptor 9 and therapeutic uses thereof Download PDF

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Publication number
WO2011009015A1
WO2011009015A1 PCT/US2010/042210 US2010042210W WO2011009015A1 WO 2011009015 A1 WO2011009015 A1 WO 2011009015A1 US 2010042210 W US2010042210 W US 2010042210W WO 2011009015 A1 WO2011009015 A1 WO 2011009015A1
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chosen
hydroxy
hydrogen
iii
hydrocarbyl
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PCT/US2010/042210
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English (en)
French (fr)
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Bobby N. Trawick
David W. Berberich
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Mallinckrodt Inc
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Mallinckrodt Inc
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Priority to DK10736929.0T priority Critical patent/DK2453895T3/en
Priority to CA2768236A priority patent/CA2768236C/en
Priority to NO10736929A priority patent/NO2453895T3/no
Priority to EP10736929.0A priority patent/EP2453895B1/en
Priority to JP2012520805A priority patent/JP5864417B2/ja
Priority to PL10736929T priority patent/PL2453895T3/pl
Priority to ES10736929.0T priority patent/ES2674018T3/es
Priority to AU2010273253A priority patent/AU2010273253B2/en
Publication of WO2011009015A1 publication Critical patent/WO2011009015A1/en
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Definitions

  • the present invention generally relates to compounds and methods for treating inflammation, pain, and other disorders.
  • the invention relates to ⁇ +)-morphinan compounds comprising Toll-like receptor 9 (TLR9) antagonist activity and methods of using the compounds to treat conditions associated with pain and inflammation.
  • TLR9 Toll-like receptor 9
  • TLRs Toll-like receptors
  • PAMPs pathogen-associated molecular patterns
  • TLRs Activation of TLRs in the central nervous system is known to initiate protective pro-inflammatory signaling cascades as part of the first line of defense against invading pathogens. Additionally, it has been reported that chronic administration of morphine or other opioid-receptor agonists activates glial cells, causing the release of pro-inflammatory factors that counter the pain-relieving effects of the opioid. Activated glial cells have also been shown to play a role in driving chronic pain states such as neuropathic pain. Given these newly identified roles for glial cells in pain, there is a need for the development of clinically useful agents that target glial cell activation as a means of pain control.
  • the present invention provides ⁇ +)-morphinan compounds that inhibit the activation of Toll-like receptor 9 (TLR9), and consequently block glial eel! activation.
  • TLR9 Toll-like receptor 9
  • the compounds of the invention may be used to treat conditions such as traumatic pain, neuropathic pain, inflammatory disorders, acetaminophen toxicity, autoimmune disorders, neurodegenerative disorders, and cancer.
  • One aspect of the invention encompasses a compound or a pharmaceutically acceptable salt thereof selected from the group consisting of 1-1 , I-2, I- 3, I-4, I-5, I-6, I-7, I-8, 1-10, 1-11 , 1-12, 1-13, 1-14, 1-16, 1-17, 1-18, 1-19, I-20, 1-21 , I-22, I- 23, I-24, ]-25, (-26, I-27, I-28, I-29, 11-1 , II-2, il-3, II-4, H-5, IJ-S, II-9, 11-10, 11-11 , 11-13, II- 14, 11-15, 11-16, 11-17, 11-18, 11-19, H-20, 11-21 , li-22, II-23, H-24, II-25, li-26, II-27, II-28, II- 29, »-30, 11-31 , II-32, II-33, II-35, II-36, II-37, II-38, II-40, 11-41 , II-42,
  • Another aspect of the invention provides a method for inhibiting
  • TLR9 activation comprises contacting a cell expressing TLR9 with a compound or a pharmaceutically acceptable salt thereof selected from the group consisting of 1-1 , I-2, I-3, I-4, I-5, i-6, I-7, I-8, I-9, 1-10, 1-11 , 1-12, 1-13, 1-14, 1-15, 1-16, I- 17, 1-18, 1-19, I-20, 1-21 , I-22, I-23, I-24, I-25, I-26, I-27, I-28, I-29, 11-1 , II-2, ii-3, Jl- 4, H-S, I-6, li-7, Jl-S, II-9, 11-10, 11-11 , 11-12, 11-13, 11-14, 11-15, 11-16, 11-17, 11-18, 11-19, II-20, 11-21 , H-22, II-23, il-24, II-25, II-26, II-27, II-28, II-29, II-30, 11-31 , II-32, II-33, II
  • Still another aspect of the invention provides a method for determining whether a compound will be therapeutically effective for treating a condition selected from the group consisting of traumatic pain, neuropathic pain, inflammatory disorders, acetaminophen toxicity, autoimmune disorders, neurodegenerative disorders, and cancer, wherein the compound is a (+)-morphinan.
  • the method comprises determining whether the compound inhibits TLR9 activation.
  • a further aspect of the present invention encompasses a method for treating a condition in a subject in need thereof.
  • the method comprises
  • the conditions that may be treated are selected from the group consisting of traumatic pain, neuropathic pain, inflammatory disorders, acetaminophen toxicity, autoimmune disorders, and neurodegenerative disorders.
  • Yet another aspect of the invention provides a method for treating a condition in a subject in need thereof.
  • the method comprises administering to the subject a combination of at least one compound comprising TLR9 antagonist activity and at least one additional therapeutic agent, wherein the compound is a (+)- morphinan.
  • the conditions that may be treated are selected from the group consisting of traumatic pain, neuropathic pain, inflammatory disorders, acetaminophen toxicity, autoimmune disorders, neurodegenerative disorders, and cancer.
  • FIG. 1 presents Toll-like receptor (TLR) antagonist screenings.
  • Each panel presents the results for a particular TLR. Plotted is the activity of a secreted alkaline phosphatase reporter system in optical density (OD) at 650 nm for each treatment condition, which was tested in duplicate. All agents were tested at 10 ⁇ M.
  • A presents TLR2 antagonist screening in which TLR2 was stimulated with HKLM at 10 8 cell/ml.
  • B presents TLR3 antagonist screening in which TLR3 was stimulated with poly(l:C) at 1 ⁇ g/ml.
  • C presents TLR4 antagonist screening in which TLR4 was stimulated with LPS at 100 ng/ml.
  • D presents TLR5 antagonist screening in which TLR5 was stimulated with Flagellin at 100 ng/ml.
  • (E) presents TLR7 antagonist screening in which TLR7 was stimulated with CL097 at 1 ⁇ g/ml.
  • (F) presents TLR8 antagonist screening in which TLR8 was stimulated with CL075 at 1 ⁇ g/ml.
  • (G) presents TLR9 antagonist screening in which TLR9 was stimulated with CpG ODN 2006 at 100 ng/ml.
  • FIG. 2 illustrates the analgesic effects of ( ⁇ )-naloxone on
  • (+)-morphinans block the activation of TLR9 and, consequently, the activation of glial cells.
  • (+)-morphinans comprising TLR9 antagonist activity may be used to treat pain, as well as other conditions associated with pain and inflammation.
  • the inhibition of TLR9 activation may be used as a screening tool to identify (+)-morphinans that may be therapeutically effective in treating conditions such as traumatic pain, neuropathic pain, inflammatory disorders, acetaminophen toxicity, autoimmune disorders, neurodegenerative disorders, and cancer.
  • the present invention provides (+)-morphinans comprising TLR9 antagonist activity, methods for inhibiting the activation of TLR9, screening methods for identifying therapeutically effective (+)- morphinans, and methods of using the (+)-morphinans comprising TLR9 antagonist activity to treat conditions such as traumatic pain, neuropathic pain, inflammatory disorders, acetaminophen toxicity, autoimmune disorders, neurodegenerative disorders, and cancer.
  • (+)- morphinans comprising TLR9 antagonist activity.
  • the (+)- morphinan comprises Formula (I) or a pharmaceutically acceptable salt thereof:
  • Ai and A 2 are independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkoxy, acyloxy, aryl, heteroaryl, hydroxy, hydroxyalkyl, polyhydroxyalkyl, amine, and amido, wherein when both Ai and A 2 are present, together they may form a carbocyclic ring or heterocyclic ring;
  • R and R' are independently selected from the group consisting of hydrogen, hydroxy, amine, hydrocarbyl, and substituted hydrocarbyl, wherein R' is optional, as represented by the dashed line;
  • R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , and R 12 are independently selected from the group consisting of hydrogen, hydroxy, amine, halo, hydrocarbyl, and substituted hydrocarbyl, wherein R 7 and Ai may together form a ring or a ring system selected from the group consisting of carbocyclic, heterocyclic, aryl, heteroaryl, and combinations thereof; Y is selected from the group consisting of hydrogen, hydroxy, aikoxy, acyloxy, amine, and amido; and
  • the dashed iines between the carbons atoms at positions 5 and 6, 6 and 7, 7 and 8, and 8 and 14 represent carbon-carbon single bonds, carbon-carbon double bonds, or combinations thereof, provided that if there is a double bond between the carbons at positions 5 and 6 then only one of R 5 or R 6 is present, if there is a double bond between the carbons at 6 and 7 then only one of R 7 or R 8 is present, if there is a double bond between the carbons at 7 and 8 then only one of R 7 or R 8 is present and only one of R 9 or R 10 is present, and if there is a double bond between the carbons at 8 and 14 then only one of R 9 or R 10 is present and Y is not present.
  • R 8 , R 9 , R 10 , R 1 1 , and R 12 are independently selected from the group consisting of hydrogen, hydroxy, alky!, alkenyl, alkynyl, aminoaikyl, alkoxyalkyl, aralkyl, cydoalkyl, hydroxyalkyl, acyloxy, aikoxy, haloalkoxyl, aryl, amine, amido, and halo.
  • R 2 , R 6 , R 8 , R 9 , R 10 , R 11 , and R 12 are hydrogen;
  • R is selected from the group consisting of hydrogen, methyl, alkyl, alkenyl, allyl, methylcycloalkyl, methyicyclopropyl, methylcyclobutyl, methylaryl, methylphenyl, acyl, acylalkyl, acylcycloalkyi, acylcyclopropyl, acylcyclobutyl, acylaryl, acylphenyl, acyloxy, acyloxyalkyl, acyloxyaryl, acyloxyphenyl, aikoxy, and alkoxyalkyl;
  • R 1 is selected from the group consisting of hydrogen, halo, alkyl, alkenyl, alkoxyalkyl, alkoxyalkenyl, aryl, heteroaryl, and furanyl;
  • R 3 and R 4 are independently selected from the group consisting of hydroxy, aikoxy, methoxy, acyloxy, and protected hydroxy;
  • R 7
  • (+)-morphinan comprises Formula (II) or a pharmaceutically acceptable salt thereof:
  • Ai and A 2 are independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkoxy, acyloxy, aryi, heteroaryl, hydroxy, hydroxyalkyi, polyhydroxyalkyl, amine, and amido, wherein when both Ai and A 2 are present, together they may form a carbocyclic ring or heterocyclic ring;
  • R and R' are independently selected from the group consisting of hydrogen, hydroxy, amine, hydrocarbyl, and substituted hydrocarbyl, wherein R' is optional, as represented by the dashed line;
  • R 1 , R 2 , R 3 , R 7 , R 8 , R 9 , R 10 , R 11 , and R 12 are independently selected from the group consisting of hydrogen, hydroxy, amine, halo, hydrocarbyl, and substituted hydrocarbyl, wherein R 7 and A 1 may together form a ring or a ring system selected from the group consisting of carbocyclic, heterocyclic, aryl, heteroaryl, and combinations thereof;
  • Y is selected from the group consisting of hydrogen, hydroxy, alkoxy, acyioxy, amine, and amido;
  • the dashed lines between the carbons atoms at positions 6 and 7, 7 and 8, and 8 and 14 represent carbon-carbon single bonds, carbon-carbon double bonds, or combinations thereof, provided that if there is a double bond between the carbons at 6 and 7 then only one of R 7 or R 8 is present, if there is a double bond between the carbons at 7 and 8 then only one of R 7 or R 8 is present and only one of R 9 or R 10 is present and if there is a double bond between the carbons at 8 and 14 then only one of R 9 or R 10 is present and Y is not present; and
  • the carbons at positions 6 and 14 may be linked by a moiety selected from the group consisting of ether, alkyl, alkenyl, substituted alkyl, and substituted alkenyl.
  • R 11 , and R 12 are independently selected from the group consisting of hydrogen, hydroxy, alkyl, alkenyl, alkynyl, aminoalky!, alkoxyalkyl, aralkyl, cycloalkyl, hydroxyalkyl, acyioxy, alkoxy, haloalkoxyi, aryl, amine, amido, and halo.
  • R 2 , R 8 , R 9 , R 10 , R 1 ⁇ and R 12 are hydrogen; R is selected from the group consisting of hydrogen, methyl, alkyl, alkenyl, allyl,
  • R 1 is selected from the group consisting of hydrogen, halo, alkyl, alkenyl, alkoxyalkyl, alkoxyaikenyl, aryl, heteroaryl, and furanyi
  • R 3 is selected from the group consisting of hydroxy, alkoxy, methoxy, acyioxy, and protected hydroxy
  • R 7 is selected from the group consisting of hydroxy, alkoxy, methoxy, acyioxy, protected hydroxy, hydrocarbyl, and substituted hydrocarbyl
  • the compound having TLR9 antagonist activity comprises Formula (III) or a pharmaceutically acceptable salt thereof:
  • R and R' are independently selected from the group consisting of hydrogen, hydroxy, amine, hydrocarbyl, and substituted hydrocarbyl, wherein R' is optional, as represented by the dashed line;
  • R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , and R 12 are independently selected from the group consisting of hydrogen, hydroxy, amine, halo, hydrocarbyl, and substituted hydrocarbyl.
  • R 8 , R 9 , R 10 , R 11 , and R 12 are independently selected from the group consisting of hydrogen, hydroxy, alky!, alkenyi, alkynyl, aminoalkyl, alkoxyalkyl, aralkyl, cycloalkyl, hydroxyalkyl, acyioxy, alkoxy, haloalkoxyl, ary!, amine, amido, and haio.
  • R 1 , R 2 , R 5 , R 6 , R 8 , R 9 , R 10 , R 11 , and R 12 are hydrogen;
  • R is selected from the group consisting of hydrogen, methyl, alkyl, alkenyi, allyi, methylcycloalkyl, methylcyclopropyl, methylcyclobutyl, methylaryl, methylphenyi, acyl, acyialkyl, acylcycloalkyl, acylcyclopropyl, acylcyclobutyl, acylaryl, acylphenyl, acyioxy, acyloxyalkyl, acyloxyaryl, acyloxyphenyl, alkoxy, and alkoxyalkyl; and R 3 , R 4 , and R 7 are independently selected from the group consisting of hydroxy, alkoxy, methoxy, acyloxy, and protected hydroxy.
  • Table C presents exemplary compounds comprising Formula (III).
  • compositions comprising Formulas (I), (II), or (III) may be provided as pharmaceuticaily-acceptable salts.
  • pharmaceutically-acceptable salt refers to a salt commonly used to form an alkali metal salt or addition salt of a free acid or a free base. The nature of the salt may vary, provided that it is pharmaceutically acceptable. Suitable pharmaceutically acceptable acid addition salts of compounds of the present invention may be prepared from an inorganic acid or from an organic acid. Examples of such inorganic acids are hydrochloric, hydrobromic, hydroiodic, nitric, carbonic, sulfuric and phosphoric acid.
  • Appropriate organic acids may be selected from aliphatic, cycloaiiphatic, aromatic, araliphatic, heterocyclic, carboxylic and sulfonic classes of organic acids, examples of which are formic, acetic, propionic, succinic, glycolic, gluconic, lactic, malic, tartaric, citric, ascorbic, glucuronic, maleic, fumaric, pyruvic, aspartic, glutamic, benzoic, anthranilic, mesylic, 4-hydroxybenzoic,
  • phenylacetic mandelic, embonic (pamoic), methanesulfonic, ethanesulfonic, benzenesulfonic, pantothenic, 2-hydroxyethanesulfonic, toluenesulfonic, sulfanilic, cyclohexylaminosulfonic, stearic, algenic, hydroxybutyric, salicylic, galactaric and gatacturonic acid.
  • Suitable pharmaceutically-acceptable base addition salts of compounds of the present invention include metallic salts made from aluminum, calcium, lithium, magnesium, potassium, sodium and zinc or organic salts made from N, N'-dibenzylethylenediamine, chloroprocaine, choline, diethanoiamine, ethylenediamine, meglumine- (N-methylgJucamtne) and procaine. All of these salts may be prepared by conventional means from the corresponding compound by reacting, for example, the appropriate acid or base with the any of the compounds of the invention.
  • Each of the compounds comprising Formulas (I), (II), or (III) has a
  • each chiral carbon has an R or an S configuration.
  • R or S configuration for a given compound will change depending on the particular Formula, i.e., (I), (II), or (III), and the compound's substitution pattern.
  • Another aspect of the present invention encompasses methods for treating a condition in a subject.
  • the method comprises administering to the subject at least one (+)-morphinan comprising TLR9 antagonist activity either alone or in combination with at least one additional therapeutic agent.
  • the compound comprises Formulas (I), (II), (III), or a pharmaceutically acceptable salt thereof.
  • disorders or disease states may be treated with the compounds of the invention. Suitable disorders and diseases that may be treated include pain conditions, inflammatory disorders, acetaminophen toxicity, autoimmune disorders,
  • the subject to be treated may be any subject having one of the indicated conditions.
  • the subject to be treated may be in need of treatment for the condition. That is, the subject has been diagnosed with the condition or is at risk for developing the condition.
  • the subject may be diagnosed with the condition using diagnostic or clinical tests that are well known.
  • diagnostic or clinical tests are used to diagnosis the different diseases or disorders.
  • the diagnostic tools include, without limit, physical examination, patient history, screening tests, laboratory tests, molecular tests, genomic tests, imaging tools, physical tests, mental tests, and the like.
  • the subject will be a human. Without departing from the scope of the invention, however, other mammalian subjects may be used. Suitable mammalian subjects include; companion animals, such as cats and dogs; livestock animals, such as cows, pigs, horses, sheep, and goats; zoo animals; and research animals, such as non-human primates and rodents.
  • pain refers to the unpleasant sensory and emotional experience associated with actual or perceived tissue damage by a noxious stimulus.
  • the pain may be acute or chronic pain.
  • the pain may be traumatic or inflammatory pain, which results from injury to non-neural tissue.
  • Non-limiting examples of traumatic or inflammatory pain include arachnoiditis, arthritis, back pain, burn pain, central pain syndrome, cancer pain, headaches (including migraines, cluster, and tension headaches); head and facial pain, muscle pain (including fibromyalgia), myofascial pain syndromes; reflex sympathetic dystrophy syndrome, repetitive stress injuries, sciatica, shingles and other skin disorders, sports injuries, spinal stenosis, surgical pain, temporomandibular disorders, trauma, and/or vascular disease or injury.
  • the pain may be neuropathic pain, which results from injury to or inflammation of the central or peripheral nervous system.
  • Neuropathic pain may occur in any part of the body and is frequently described as a hot, burning sensation, which can be devastating to the affected individual.
  • Neuropathic pain may be acute or chronic; it may result from diseases that affect nerves (such as diabetes), from trauma, surgical procedures, arthritis, AIDS, burn injuries, cerebral or lumbar spine disease, fibromyalgia, post-eschemic pain, tumors, viral neuralgias, or, because chemotherapy drugs can affect nerves, it may be a consequence of cancer treatment.
  • diabetic neuropathy which results from nerve damage secondary to vascular problems that occur with diabetes
  • reflex sympathetic dystrophy syndrome which may follow injury
  • phantom limb and post- amputation pain which may result from the surgical removal of a limb
  • post-herpetic neuralgia which may occur after an outbreak of shingles
  • complex regional pain syndrome or central pain syndrome which may result from trauma to the brain or spinal cord.
  • Characteristic symptoms of neuropathic pain include hyperesthesia
  • ailodynia i.e., widespread tenderness or hypersensitivity to tactile stimuli
  • hyperalgesia i.e., abnormal sensitivity to pain
  • spontaneous burning pain i.e., spontaneous burning pain
  • phantom pain i.e., perception of pain that is nonexistent.
  • Hyperesthesia involves an unusual increased or altered sensitivity to sensory stimuli, including for example, acoustic, cerebral, gustatory, muscular, olfactory, oneiric, optic, or tactile.
  • a painful sensation from a normally painless touch stimulus Ailodynia involves an intensified, unpleasant, and painful perception of stimuli triggered by heat or by contact, which is based on a lowering of the pain threshold for these stimuli, including, for example, a non-noxious stimulus to normal skin.
  • Hyperalgesia involves the excessive perception of a variety of stimuli, again based on a lowering of the pain threshold and thus an abnormally increased pain sense, including for example, auditory or muscular stimuli.
  • Phantom pain involves a perception of pain in a ⁇ mb that is non-existent, such as perceived pain in a limb that has been amputated, i.e. phantom limb syndrome.
  • H inflammatory disorders
  • the inflammatory disorder may be arthritis including, but not limited to, rheumatoid arthritis, spondyloarthropathies, gouty arthritis, osteoarthritis, systemic lupus erythematosus, or juvenile arthritis.
  • arthritis including, but not limited to, rheumatoid arthritis, spondyloarthropathies, gouty arthritis, osteoarthritis, systemic lupus erythematosus, or juvenile arthritis.
  • the inflammation may be associated with asthma, allergic rhinitis, sinus diseases, bronchitis, tuberculosis, acute pancreatitis, sepsis, infectious diseases, menstrual cramps, premature labor, tendinitis, bursitis, skin-related conditions such as psoriasis, eczema, atopic dermatitis, urticaria, dermatitis, contact dermatitis, and burns, or from post-operative inflammation including from ophthalmic surgery such as cataract surgery and refractive surgery.
  • the inflammatory disorder may be a gastrointestinal condition such as inflammatory bowe!
  • the inflammation may be associated with diseases such as vascular diseases, migraine headaches, periarteritis nodosa, thyroiditis, aplastic anemia, Hodgkin's disease, sclerodoma, rheumatic fever, type I diabetes,
  • the inflammatory disorder may be associated with an ophthalmic disease, such as retinitis, retinopathies, uveitis, ocular photophobia, or of acute injury to the eye tissue.
  • the inflammation may be a pulmonary inflammation, such as that associated with viral infections or cystic fibrosis, chronic obstructive pulmonary disease, or acute respiratory distress syndrome.
  • the inflammatory disorder may also be associated with tissue rejection, graft v. host diseases, delayed-type hypersensitivity, as well as immune-mediated and inflammatory elements of CNS diseases such as Alzheimer's, Parkinson's, multiple sclerosis, and the like.
  • acetaminophen toxicity also known as paracetamol toxicity
  • High levels of acetaminophen may lead to damage of the liver (i.e., acetaminophen-induced hepatotoxicity) or the kidney (i.e., acetaminophen-induced nephrotoxicity).
  • Acetaminophen toxicity may result from an acute overdose of acetaminophen or a chronic overdose acetaminophen.
  • the amount of ingested acetaminophen at which toxicity occurs may be reduced upon chronic ethanol use, malnourisnment, or diminished nutritional status, fasting, or viral illness with dehydration, or use of certain pharmaceutical agents that interact with the enzyme systems that metabolize acetaminophen.
  • Acetaminophen-induced hepatotoxicity may be manifested by cellular oxidative damage, mitochondrial dysfunction, and a subsequent inflammatory response.
  • Cellular damage may be monitored by elevated levels of serum alanine transaminase (ALT) or serum aspartate transaminase (AST), and the inflammatory response may be monitored by increased levels of pro-interleukin(lL)-1 beta transcript levels.
  • ALT serum alanine transaminase
  • AST serum aspartate transaminase
  • Acetaminophen-induced hepatotoxicity also may iead to hepatocellular injury, death, and centrilobular (zone III) liver necrosis. Similar enzymatic reactions occur in the kidney, and may contribute to some degree of extra-hepatic organ dysfunction.
  • the autoimmune disorder may be systemic, such as Lupus, wherein many tissues or organs are affected or damaged. Alternatively, the autoimmune disorder may be localized, such as type I diabetes mellitus, wherein a single organ or tissue is damaged or affected.
  • Non-limiting examples of autoimmune disorders include acute disseminated encephalomyelitis (ADEM), Addison's disease, alopecia areata, antiphospholipid antibody syndrome (APS), autoimmune hemolytic anemia,
  • autoimmune hepatitis autoimmune inner ear disease, bullous pemphigoid, celiac disease, Chagas disease, chronic obstructive pulmonary disease, Crohn's disease, dermatomyositis, diabetes meliitus type 1 , endometriosis, Goodpasture's syndrome, Graves' disease, Guillain-Barre syndrome (GBS), Hashimoto's thyroiditis, hidradenitis suppurativa, Kawasaki disease, IgA nephropathy, idiopathic thrombocytopenic purpura, interstitial cystitis, lupus erythematosus (Lupus), mixed connective tissue disease, morphea, multiple sclerosis, myasthenia gravis, narcolepsy, neuromyotonia, pemphigus vulgaris, pernicious anemia, psoriasis, psoriatic arthritis, polymyalgia rheumatica, poly
  • neurodegenerative disorders include adrenal leukodystrophy, aging-related disorders and dementias, alcoholism, Alexander's disease, Aiper's disease, Alzheimer's disease, amyotrophic lateral sclerosis (Lou Gehrig's Disease), ataxia telangiectasia, Batten disease (also known as Spielmeyer- Vogt-Sjogren-Batten disease), bovine spongiform encephalopathy (BSE), canavan disease, cerebral paisy, Cockayne syndrome, corticobasal degeneration (CBD), Creutzfeldt-Jakob disease, familial fatal insomnia, frontotemporal lobar degeneration, frontal temporal dementias (FTDs), Huntington's disease, HIV-associated dementia, Kennedy's disease, Krabbe's disease, Lewy body disease, neuroborreliosis
  • neoplasm may be malignant or benign, the cancer may be primary or metastatic; the neoplasm or cancer may be early stage or late stage.
  • Non-limiting examples of neoplasms or cancers that may be treated include acute lymphoblastic leukemia, acute myeloid leukemia, adrenocortical carcinoma, AIDS-related cancers, AIDS-related lymphoma, anal cancer, appendix cancer, astrocytomas (childhood cerebellar or cerebral), basal cell carcinoma, bile duct cancer, bladder cancer, bone cancer, brainstem glioma, brain tumors
  • Cerebellar astrocytoma cerebral astrocytoma/malignant glioma, ependymoma, medulloblastoma, supratentorial primitive neuroectodermal tumors, visual pathway and hypothalamic gliomas
  • breast cancer bronchial adenomas/carcinoids
  • Burkitt lymphoma carcinoid tumors (childhood, gastrointestinal), carcinoma of unknown primary, central nervous system lymphoma (primary), cerebellar astrocytoma, cerebral astrocytoma/malignant glioma, cervical cancer, childhood cancers, chronic lymphocytic leukemia, chronic myelogenous leukemia, chronic myeloproliferative disorders, colon cancer, cutaneous T-cell lymphoma, desmoplastic small round ceil tumor, endometrial cancer, ependymoma, esophageal cancer, Ewing's sarcoma in the Ewing family of tumors,
  • bone/osteosarcoma medulloblastoma (childhood), melanoma, intraocular melanoma, Merkel cell carcinoma, mesotheliomas (adult malignant, childhood), metastatic squamous neck cancer with occult primary, mouth cancer, multiple endocrine neoplasia syndrome (childhood), multiple myeloma/plasma cell neoplasm, mycosis fungoides, myelodysplastic syndromes, myelodysplastic/myeloproliferative diseases, myelogenous leukemia (chronic), myeloid leukemias (adult acute, childhood acute), multiple myeloma, myeloproliferative disorders (chronic), nasal cavity and paranasal sinus cancer, nasopharyngeal carcinoma, neuroblastoma, non-Hodgkin lymphoma, non-small cell lung cancer, oral cancer, oropharyngeal cancer, osteosarcoma/malignant fibrous his
  • the treatment method comprises
  • the TLR9 antagonist is a compound comprising Formulas (1), (II), (Mf), or a pharmaceutically acceptable salt thereof. Suitable pharmaceutically acceptable salts are detailed above in section (f)(d).
  • compositions may be administered orally, parenterally, by inhalation spray, rectaliy, intradermally, intrathecally, transdermal ⁇ , or topically in dosage unit formulations containing conventional nontoxic pharmaceutically acceptable carriers, adjuvants, and vehicles as desired.
  • Topical administration may also involve the use of transdermal administration such as transdermal patches or iontophoresis devices.
  • Formulation of therapeutic agents is discussed in, for example, Gennaro, A. R., Remington's Pharmaceutical Sciences, Mack Publishing Co., Easton, Pa. (18th ed, 1995), and Liberman, H. A. and Lachman, L., Eds., Pharmaceutical Dosage Forms, Marcel Dekker Inc., New York, N.Y. (1980).
  • Preparations for oral administration generally contain inert excipients in addition to the active pharmaceutical ingredient.
  • Oral preparations may be enclosed in gelatin capsules or compressed into tablets.
  • Common excipients used in such preparations include pharmaceutically compatible fillers/diluents such as microcrystalline cellulose, hydroxypropyl methylce ⁇ ulose, starch, lactose, sucrose, glucose, mannitol, sorbitol, dibasic caicium phosphate, or calcium carbonate; binding agents such as alginic acid, carboxymethylcellulose, microcrystalline cellulose, gelatin, gum tragacanth, or polyvinylpyrrolidone; disintegrating agents such as alginic acid, cellulose, starch, or polyvinylpyrrolidone; lubricants such as calcium stearate, magnesium stearate, talc, silica, or sodium stearyl fumarate; glidants such as colloidal silicon dioxide; sweetening agents such as sucrose or saccharin;
  • Oral preparations may also be administered as aqueous suspensions, elixirs, or syrups.
  • the active ingredient may be combined with various sweetening or flavoring agents, coloring agents, and, if so desired, emulsifying and/or suspending agents, as well as diluents such as water, ethanol, glycerin, and combinations thereof.
  • the preparation may be an aqueous or an oil-based solution.
  • Aqueous solutions may include a sterile diluent such as water, saline solution, a pharmaceutically acceptable polyoi such as glycerol, propylene glycol, or other synthetic solvents; an antibacterial and/or antifungal agent such as benzyl alcohol, methyl paraben, chlorobutanol, phenol, thimerosal, and the like; an antioxidant such as ascorbic acid or sodium bisulfite; a chelating agent such as
  • etheylenediaminetetraacetic acid etheylenediaminetetraacetic acid
  • a buffer such as acetate, citrate, or phosphate
  • an agent for the adjustment of tonicity such as sodium chloride, dextrose, or a polyalcoho! such as mannitoi or sorbitol.
  • the pH of the aqueous solution may be adjusted with acids or bases such as hydrochloric acid or sodium hydroxide.
  • Oil-based solutions or suspensions may further comprise sesame, peanut, olive oil, or mineral oil.
  • transdermal or transmucosal administration penetrants appropriate to the barrier to be permeated are generally included in the preparation.
  • Transmucosal administration may be accomplished through the use of nasal sprays, aerosol sprays, tablets, or suppositories, and transdermal administration may be via ointments, salves, gels, patches, or creams as generally known in the art.
  • the amount of agent that is administered to the subject can and will vary depending upon the type of agent, the subject, and the particular mode of administration. Those skilled in the art will appreciate that dosages may also be determined with guidance from Goodman & Goldman's The Pharmacological Basis of Therapeutics, Tenth Edition (2001), Appendix II, pp. 475-493, and the Physicians' Desk Reference.
  • the treatment method comprises
  • the TLR9 antagonist is a compound comprising Formulas (I), (II), (III), or a pharmaceutically acceptable salt thereof. It is envisioned that when the combination formulation comprises more than one additional therapeutic agent, the therapeutic agents may be drawn from one of the classes listed below, or the therapeutic agents may be drawn from different classes listed below.
  • the analgesic may be an (-)-opioid analgesic.
  • the analgesic may be a non-opioid analgesic.
  • suitable opioid analgesics include buprenorphine, butorphanol, codeine,
  • the concentration or dose of the opioid analgesic in the combination formulation may be sub-analgesic.
  • non-opioid analgesics include without limit acetylsalicylic acid, acetaminophen (paracetamol), ibuprofen, ketoprofen, indomethacin, diflunisol, naproxen, ketorolac, dichlophenac, tolmetin, sulindac, phenacetin, piroxicam, and mefamanic acid
  • the analgesic may comprise a combination of an opiate analgesic and a non-opioid analgesic.
  • acetaminophen may be combined with codeine, hydrocodone, oxycodone, propoxyphene, or another opioid analgesics.
  • the combination may comprise a compound comprising Formulas (I), (fl), or (III), and acetaminophen.
  • concentration of acetaminophen in such a combination may be lower than in currently available acetaminophen combination formulations.
  • the anti-inflammatory agent may be a glucocorticoid steroid such as the naturally occurring hydrocortisone (Cortisol), or synthetic glucocorticoids such as prednisone, prednisolone, methylprednisolone, dexamethasone, betamethasone, triamcinolone, beclometasone, fludrocortisones, deoxycorticosterone, alcJometasone, fluocinonide, aldosterone, and derivatives thereof.
  • the anti-inflammatory agent may be a non-steroidal anti-inflammatory agent (NSAID).
  • Non-limiting examples of suitable NSAiDs include acetylsalicylic acid (aspirin), celecoxib, choline magnesium salicylate, Cox-2 inhibitors, diclofenac, diflunisal, etodolac, fenoprofen, flufenisal, flurbiprofen, ibuprofen, indomethacin, ketoprofen, ketorolac, meclofenamate, mefenamate, nabumetone, naproxen, oxaprozin, phenylbutazone, piroxicam, salsalate, sulindac, tolmetin, valdecoxib, and zomepirac.
  • acetylsalicylic acid aspirin
  • celecoxib celecoxib
  • choline magnesium salicylate Cox-2 inhibitors
  • diclofenac diflunisal
  • etodolac fenoprofen
  • antibiotic agents include aminoglycosides such as, e.g., amikacin, gentamicin, kanamycin, neomycin, netilmicin, streptomycin, and tobramycin; a carbecephem such as loracarbef;
  • carbapenems such as, e.g., certapenem, imipenem, and meropenem
  • cephalosporins such as, e.g., cefadroxil cefazolin, cephalexin, cefaclor, cefamandole, cephalexin, cefoxitin, cefprozil, cefuroxime, cefixime, cefdinir, cefditoren, cefoperazone, cefotaxime, cefpodoxime, ceftazidime, ceftibuten, ceftizoxime, and ceftriaxone
  • macrolides such as, e.g., azithromycin, clarithromycin, dirithromycin, erythromycin, and troleandomycin
  • penicillins such as, e.g., amoxicillin, ampicillin, carbenicillin, cloxacillin, dicloxacillin, nafcillin, oxacillin
  • polypeptides such as, e.g., bacitracin, coiistin, and polymyxin B; quinolones such as, e.g., ciprofloxacin, enoxacin, gatifloxacin, levofloxacin, lomefloxacin, moxifloxacin, norfloxacin, ofloxacin, and trovafloxacin; sulfonamides such as, e.g., mafenide, sulfacetamide, sulfamethizole, sulfasalazine, sulfisoxazoie, and trimethoprim- sulfamethoxazole; tetracyclines such as, e.g., demeclocycline, doxycycline,
  • minocycline and oxytetracycline
  • an antimicrobial agent such as, e.g., sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite, sodium metabisulfite
  • ketoconazole amoxicillin, cephalexin, miconazole, econazole, acyclovir, and nelfinavir.
  • Formulas (I), (II), (III), or a pharmaceutically acceptable salt thereof may be administered in combination with an agent used to treat acetaminophen toxicity.
  • Suitable agents include acetylcysteine ⁇ also called N-acetylcysteine), glutathione, and activated charcoal.
  • autoimmune therapeutic agents include immunosuppressants such as azathioprine, chlorambucil, cyclophosphamide, cyclosporine, mycophenolate, or methotrexate;
  • corticosteroids such as prednisone; the psoriasis treatment agent alefacept; TNF blockers such as etanercept, infliximab, or adalimumab; white blood cell blockers such as abatacept or ritaximab; the leprosy drug clofazimine; and chemotherapeutic agents such as vorinostat.
  • the neurodegenerative disorder therapeutic agent is tailored to the specific
  • Suitable therapeutic agents for the treatment of Parkinson disease include, without limit, ievadopa (i.e., L-DOPA); a decarboxylase inhibitor such as carbidopa; a direct acting dopamine agonist such bromocriptine, pergolide, ropinirole or pramipexole; a dopamine uptake inhibitor such as amantadine; an anticholinergic such as trihexyphenidyl or benztropine mesylate; a monoamine oxidase B inhibitor such as L-deprenyl; a catechol-O-methyltranferase inhibitor such as tolcapone; spheramine; and combinations thereof.
  • ievadopa i.e., L-DOPA
  • a decarboxylase inhibitor such as carbidopa
  • a direct acting dopamine agonist such bromocriptine, pergolide, ropinirole or pramipexole
  • Non-limiting examples of suitable therapeutic agents for the treatment of Alzheimer's disease include cholinesterase inhibitors such as donepezil, rivastigmine, galantamine, and the like; NMDA receptor antagonists such as memantine; and Alzheimer's specific agents such as tramiprosate, tarenflubil, phenserine, and the like.
  • cholinesterase inhibitors such as donepezil, rivastigmine, galantamine, and the like
  • NMDA receptor antagonists such as memantine
  • Alzheimer's specific agents such as tramiprosate, tarenflubil, phenserine, and the like.
  • Huntington's disease include, with out limit, tetrabenazine, xenazine, and so forth.
  • Non- limiting examples of therapeutic agents targeted to treat amyotrophic lateral sclerosis (ALS) include riluzole, mecasermin rinfabate, and the like.
  • the chemotherapeutic agent may be a cytotoxic agent that affects rapidly dividing cells in general, or it may be a targeted therapeutic agent that affects the deregulated proteins of cancer cells.
  • the chemotherapeutic agent may be an alkylating agent, an anti-metabolite, an anti-tumor antibiotic, an anti-cytoskeletal agent, a topoisomerase inhibitor, an anti-hormonal agent, a targeted therapeutic agent, or a combination thereof.
  • alkylating agents include altretamine, benzodopa, busulfan, carboplatin, carboquone, carmustine, chlorambucil, chlornaphazine, cholophosphamide, chlorozotocin, cisplatin, cyclosphosphamide, dacarbazine (DTIC), estramustine, fotemustine, ifosfamide, improsulfan, lomustine, mechlorethamine, mechlorethamine oxide hydrochloride, melphalan, meturedopa, nimustine, novemb ⁇ chin, phenesterine, piposulfan,
  • DTIC dacarbazine
  • estramustine fotemustine
  • ifosfamide improsulfan
  • lomustine mechlorethamine, mechlorethamine oxide hydrochloride, melphalan, meturedopa, nimustine, novemb ⁇ chin,
  • Suitable anti-metabolites include, but are not limited to aminopterin, ancitabine, azacitidine, 6-azauridine, capecitabine, carmofur, cytarabine or cytosine arabinoside (Ara-C), dideoxyuridine, denopterin, doxifluridine, enocitabine, floxuridine, fludarabine, 5-fluorouracil (5-FU), gemcetabine, leucovorin (folinic acid), 6-mercaptopurine, methotrexate, pemetrexed, pteropterin, thiamiprine, trimetrexate, and thioguanine.
  • Non-limiting examples of suitable anti-tumor antibiotics include aclacinomysin, actinomycin, adriamycin, authramycin, azaserine, bleomycins, cactinomycin, calicheamicin, carabicin, caminomycin, carzinophilin, chromomycins, dactinomycin, daunorubicin, detorubicin, 6-diazo-5-oxo-L-norleucine, doxorubicin, epirubicin, esorubicin, idarubicin, marcellomycin, mitomycins, mycophenoiic acid, nogalamycin, olivomycins, peplomycin, potfiromycin, puromycin, quelamyci ⁇ , rodorubicin, streptonigrin, streptozocin, tubercidin, ubenimex, zinostatin, and zorubicin.
  • Non-limiting examples of suitable anti-cytoskeletal agents include colchicines, docetaxe!, macromycin, paclitaxel (taxol), vinblastine, vincristine, vindesine, and vinorelbine.
  • Suitable topoisomerase inhibitors include, but are not limited to, amsacrine, etoposide (VP-16), irinotecan, RFS 2000, teniposide, and topotecan.
  • Non-limiting examples of suitable anti-hormonal agents such as aminoglutethimide, aromatase inhibiting 4(5)-imidazoles, bicalutamide, finasteride, flutamide, goserelin, 4- h yd roxy tamoxifen, keoxifene, leuprolide, LY117018, mitotane, nilutamide, onapristone, raloxifene, tamoxifen, toremifene, and trilostane.
  • suitable anti-hormonal agents such as aminoglutethimide, aromatase inhibiting 4(5)-imidazoles, bicalutamide, finasteride, flutamide, goserelin, 4- h yd roxy tamoxifen, keoxifene, leuprolide, LY117018, mitotane, nilutamide, onapristone, raloxifene, tamoxi
  • Non-limiting examples of targeted therapeutic agents include a monoclonal antibody such as alemtuzumab, bevacizumab, capecitabine, cetuximab, gemtuzumab, heregulin, rituximab, trastuzumab; a tyrosine kinase inhibitor such as imatinib mesylate; and a growth inhibitory polypeptide such as erythropoietin, interleukins (e.g., 1L-1 , IL-2, IL-3, IL-6), leukemia inhibitory factor, interferons, thrombopoietin, TNF- ⁇ , CD30 Itgand, 4-1 BB ligand, and Apo-1 iigand.
  • erythropoietin interleukins
  • interleukins e.g., 1L-1 , IL-2, IL-3, IL-6
  • leukemia inhibitory factor interferons
  • a further aspect of the present invention provides methods for inhibiting the activation of TLR9.
  • the method comprises contacting a cell expressing TLR9 with a compound comprising Formulas (I), (M), (III), or a
  • the method comprises contacting the cell with a compound selected from the group consisting of 1-1 , 1-2, I-3, I- 4, I-5, I-6, I-7, I-8, I-9, 1-10, 1-11 , 1-12, 1-13, 1-14, 1-15, 1-16, 1-17, 1-18, 1-19, I-20, 1-21 , I- 22, I-23, I-24, I-25, I-26, i-27, I-28, I-29, IM , II-2, li-3, II-4, II-5, I-6, H-7, II-8, II-9, IMO, II- 11, 11-12, 11-13, 11-14, 11-15, 11-16, 11-17, 11-18, 11-19, H-20, 11-21 , II-22, H-23, il-24, II-25, II- 26, II-27, II-28, li-29, II-30, 11-31, il-32, II-33, II-34, II-35, II-36, II-37, II
  • the method of inhibiting the activation of TLR9 may be conducted in vivo or it may be conducted in vitro. Accordingly, the cell expressing TLR9 may be disposed in a subject as detailed above.
  • the cell may be a glial cell, a microglial cell, or an astrocyte. In an exemplary embodiment, the cell may be a glial cell in the central nervous system.
  • the present invention afso provides a method for identifying a compound comprising Formulas (I), (II), or (III) that may be therapeutically effective for treating conditions associated with pain and inflammation. Suitable conditions include traumatic or neuropathic pain, an inflammatory disorder, acetaminophen toxicity, an autoimmune disorder, a neurodegenerative disorder, and cancer. The method comprises determining whether the compound inhibits TLR9 activation.
  • the method comprises contacting a cell expressing TLR9 with an activation ligand and the compound of interest, wherein TLR9 activation is reduced in the presence of the compound as compared to a control condition in which the cell is contacted with only the activation ligand.
  • the cell expressing TLR9 that is contacted with the compound of interest is /Ir? vitro.
  • the cell expressing TLR9 will be from a stable cell line.
  • Non-limiting examples of suitable parental cells include HEK293, CHO, BHK, NSO, HDJvIEC 1 NHEK, and NHDF cells.
  • the cell line may be HEK293.
  • the cells may be engineered to express TLR9 using standard procedures well known to those of skill in the art.
  • TLR9 may be of mammalian origin, preferably of human origin.
  • the activation ligand used to activate TLR9 may be methylated
  • the activation ligand may be CpG o ⁇ godeoxynucleotide (ODN) 2006.
  • the activation of TLR9 in the cell of the in vitro assay may be monitored by measuring the activity of a reporter, wherein the activity of the reporter is coupled to activation of an adaptor protein or kinase that mediates TLR9 signaling by producing intracellular signaling molecules or inducers such as NF- ⁇ B or IRF3.
  • suitable reporters include luciferase, alkaline phosphatase, and GFP or other fluorescent proteins.
  • the activation of the reporter may be monitored via luminescence, fluorescence, absorbance, or optical density.
  • the reporter is secreted alkaline phosphatase (SEAP) that is induced by NF-KB, and activation of SEAP is monitored spectrophotometically.
  • SEAP alkaline phosphatase
  • the (+)-morphinan comprising TLR9 antagonist activity may reduce the activation of TLR9 by at least about 10%.
  • the (+)-morphinan may reduce the activation of TLR9 from about 10% to about 15%, from about 15% to about 20%, from about 20% to about 25%, from about 25% to about 30%, from about 30% to about 35%, from about 35% to about 40%, from about 40% to about 45%, from about 45% to about 50%, from about 50% to about 55%, from about 55% to about 60%, from about 60% to about 70%, from about 70% to about 80%, from about 80% to about 90%, or from about 90% to about 99%.
  • the in vitro screening assay may also be used to determine the optimal inhibitory concentration (or IC 5 o) of a (+)-morphinan comprising TLR9 antagonist activity. That is, a dose-response curve may be generated in which the concentration of the (+)-morphinan comprising TLR9 antagonist activity is varied such that the optimal inhibitory concentration may be determined.
  • acyl denotes the moiety formed by removal of the hydroxy group from the group COOH of an organic carboxylic acid, e.g., RC(O)-, wherein R is R 1 , R 1 O-, R 1 R 2 N-, or R 1 S-, R 1 is hydrocarbyl, heterosubstituted hydrocarbyl, or heterocyclo, and R 2 is hydrogen, hydrocarbyl, or substituted hydrocarbyl.
  • acyloxy denotes an acyl group as described above bonded through an oxygen linkage (O), e.g., RC(O)O- wherein R is as defined in connection with the term "acyl.”
  • O oxygen linkage
  • alkyl as used herein describes groups which are preferably lower aikyl containing from one to eight carbon atoms in the principal chain and up to 20 carbon atoms. They may be straight or branched chain or cyclic and include methyl, ethyl, propyl, isopropyl, butyl, hexyi and the like.
  • alkenyl as used herein describes groups which are preferably lower alkenyl containing from two to eight carbon atoms in the principal chain and up to 20 carbon atoms. They may be straight or branched chain or cyclic and include ethenyl, propenyl, isopropenyl, butenyl, isobutenyl, hexenyl, and the like.
  • alkynyl as used herein describes groups which are preferably lower alkynyl containing from two to eight carbon atoms in the principal chain and up to 20 carbon atoms. They may be straight or branched chain and include ethynyl, propynyl, butynyl, isobutynyl, hexynyl, and the like.
  • aromatic as used herein atone or as part of another group denotes optionally substituted homo- or heterocyclic conjugated planar ring or ring system comprising delocalized electrons. These aromatic groups are preferably monocyclic (e.g., furan or benzene), bicyclic, or tricyclic groups containing from 5 to 14 atoms in the ring portion.
  • aromatic encompasses "aryl” groups defined below.
  • aryl or “Ar” as used herein alone or as part of another group denote optionally substituted homocyclic aromatic groups, preferably monocyclic or bicyclic groups containing from 6 to 10 carbons in the ring portion, such as phenyl, biphenyi, naphthyl, substituted phenyl, substituted biphenyl, or substituted naphthyl.
  • carbbocyclo or “carbocyclic” as used herein alone or as part of another group denote optionally substituted, aromatic or non-aromatic, homocyclic ring or ring system in which all of the atoms in the ring are carbon, with preferably 5 or 6 carbon atoms in each ring.
  • substituents include one or more of the following groups: hydrocarbyl, substituted hydrocarbyi, alky!, alkoxy, acyl, acyloxy, alkenyl, alkenoxy, aryl, aryloxy, amino, amido, acetal, carbamyl, carbocyclo, cyano, ester, ether, halogen, heterocyclo, hydroxy, keto, ketai, phospho, nitro, and thio.
  • halogen or halo as used herein alone or as part of another group refer to chiorine, bromine, fluorine, and iodine.
  • heteroatom refers to atoms other than carbon and hydrogen.
  • heteromatic as used herein alone or as part of another group denotes optionally substituted aromatic groups having at least one heteroatom in at least one ring, and preferably 5 or 6 atoms in each ring.
  • heteroaromatic group preferably has 1 or 2 oxygen atoms and/or 1 to 4 nitrogen atoms in the ring, and is bonded to the remainder of the molecule through a carbon.
  • Exemplary groups include furyl, benzofuryl, oxazolyl, isoxazolyl, oxadiazolyl,
  • benzoxazolyi benzoxadiazolyl, pyrrolyl, pyrazolyl, imidazolyl, triazolyl, tetrazolyl, pyridyl, pyrimidyl, pyrazi ⁇ yf, pyridaziny!, indolyl, iso ⁇ ndolyl, ⁇ ndolizinyl, benzimfdazolyl, indazolyl, benzotriazolyl, tetrazolopy ⁇ dazinyl, carbazofyl, purinyl, quinolinyl, isoquinolinyl, imidazopyridyl, and the like.
  • substituents include one or more of the following groups: hydrocarbyl, substituted hydrocarbyl, alkyl, alkoxy, acyl, acyloxy, alkenyl, alkenoxy, aryl, aryloxy, amino, amido, acetal, carbamyl, carbocyclo, cyano, ester, ether, halogen, heterocyclo, hydroxy, keto, ketal, phospho, nitro, and thio.
  • heterocyclo or “heterocyclic” as used herein alone or as part of another group denote optionally substituted, fully saturated or unsaturated, monocyclic or bicyclic, aromatic or non-aromatic groups having at least one heteroatom in at least one ring, and preferably 5 or 6 atoms in each ring.
  • the heterocyclo group preferably has 1 or 2 oxygen atoms and/or 1 to 4 nitrogen atoms in the ring, and is bonded to the remainder of the molecule through a carbon or heteroatom.
  • Exemplary heterocyclo groups include heteroaromatics as described above.
  • substituents include one or more of the following groups: hydrocarbyl, substituted hydrocarbyl, afkyl, alkoxy, acyl, acyloxy, alkenyl, alkenoxy, aryl, aryloxy, amino, amido, acetal, carbamyl, carbocydo, cyano, ester, ether, halogen, heterocyclo, hydroxy, keto, ketal, phospho, nitro, and thio.
  • hydrocarbon and “hydrocarbyl” as used herein describe organic compounds or radicals consisting exclusively of the elements carbon and hydrogen. These moieties include alkyl, alkenyl, alkynyl, and aryl moieties. These moieties also include alkyl, alkenyl, alkynyl, and aryl moieties substituted with other aliphatic or cyclic hydrocarbon groups, such as alkaryl, alkenaryl and alkynaryl. Unless otherwise indicated, these moieties preferably comprise 1 to 20 carbon atoms.
  • protecting group denotes a group capable of protecting an oxygen atom (and hence, forming a protected hydroxy), wherein the protecting group may be removed, subsequent to the reaction for which protection is employed, without disturbing the remainder of the molecule.
  • protecting groups include ethers (e.g., allyl, triphenylmethyl (trityl or Tr), p- methoxybenzyl (PMB), p-methoxyphenyl (PMP)), acetals (e.g., methoxymethyl (MOM), ⁇ -methoxyethoxymethyl (MEM), tetrahydropyranyl (THP), ethoxy ethyl (EE),
  • MTM methylthiomethyl
  • MOP 2-methoxy-2-propyl
  • SEM 2-trimethylsilylethoxymethyl
  • esters e.g., benzoate (Bz), ally! carbonate, 2,2,2-trichloroethyl carbonate (T roc), 2-trimethylsilylethyl carbonate), silyl ethers (e.g., trimethylsilyl (TMS), triethylsilyl (TES), triisopropylsilyl (TIPS), triphenyls ⁇ yi (TPS), t-butyldimethylsilyl (TBDMS), t- butyldiphenylsilyl (TBDPS) and the like.
  • TMS trimethylsilyl
  • TES triethylsilyl
  • TIPS triisopropylsilyl
  • TPS triphenyls ⁇ yi
  • TDMS t-butyldimethylsilyl
  • TDPS t- but
  • substituted hydrocarbyl moieties described herein are hydrocarbyl moieties which are substituted with at least one atom other than carbon, including moieties in which a carbon chain atom is substituted with a heteroatom such as nitrogen, oxygen, silicon, phosphorous, boron, or a halogen atom, and moieties in which the carbon chain comprises additional substituents.
  • substituents include alkyl, alkoxy, acyl, acyloxy, alkenyl, alkenoxy, aryl, aryloxy, amino, amido, acetai, carbamyl, carbocyclo, cya ⁇ o, ester, ether, halogen, heterocyclo, hydroxy, keto, ketal, phospho, nitro, and thio.
  • treating refers to inhibiting or alleviating the symptoms of the disease or disorder; reversing, inhibiting, or slowing the
  • treatment refers to the act of treating as "treating" is defined immediately above.
  • Stimulation of TLRs 2, 3, 4, 5, 7, 8, and 9 was determined by assessing activation of the transcription factor NF- ⁇ B in HEK293 cells that were engineered to express the corresponding receptors.
  • Assessment of TLR stimulation was based on the use of an NF- ⁇ B-inducible secreted alkaline phosphatase (SEAP) reporter system in which the SEAP reporter was under the control of a promoter inducible by NF- ⁇ B.
  • SEAP NF- ⁇ B-inducible secreted alkaline phosphatase
  • TLR9 TLR9 cells were plated in Growth Medium in a 96-well plate (25,000-50,000 cells/well). The cells were stimulated with the appropriate positive control Hgand or no liga ⁇ d was added (negative control).
  • the positive control ligands were: HKLM was used to stimulate TLR2; poly(l:C) was used to stimulate TLR3; LPS was used to stimulate TLR4; Flagellin was used to stimulate TLR5; CL097 was used to stimulate TLR7; CL075 was used to stimulate TLR8; CpG oligodeoxynucieotide (ODN) 2006 was used to stimulate TLR9.
  • the cells were pretreated with an antagonist for 30 minutes prior to addition of the positive control ligand. For this, 20 ⁇ L of the stock test compound solution (100 ⁇ M in H 2 O) was added to give a total volume of 200 ⁇ L.
  • the antagonists tested were (+)-naloxone, (+)- naltrexone, sinomenine, and dihydrosinomenine; the final concentration of each was 10 ⁇ M.
  • the secreted alkaline phosphatase reporter is under the control of a promoter inducible by the transcription factor NF- ⁇ B.
  • a library of more than 100 (+)- morphinan compounds was screened for TLR9 antagonist activity by assessing NF- ⁇ B activation in the HEK293 cells expressing TLR9. This reporter gene allows the monitoring of signaling through the TLR, based on the activation of NF- ⁇ B.
  • This screening experiment identified those (+)-morphinan compounds comprising the greatest TLR9 antagonist activity. Even at a concentration as low as 100 nM, some compounds inhibited TLR9 in excess of 90%.
  • Neuropathic pain affects approximately 1 % of the U.S. population and is extremely difficult to manage, Usualiy the pain is chronic, severe, and fails to respond to traditional analgesic drugs. Fortunately, one of the most widely used animal models for neuropathic pain closely mimics the pain endured by patients. In this mode!, termed the chronic constriction injury (CCI) or Bennett mode], four closely spaced ligatures tied loosely around the sciatic nerve of a rat cause demyelination of the nerve, resulting in spontaneous pain, such as prolonged paw elevation and ⁇ cking of the ligated paw.
  • CCI chronic constriction injury
  • Bennett mode four closely spaced ligatures tied loosely around the sciatic nerve of a rat cause demyelination of the nerve, resulting in spontaneous pain, such as prolonged paw elevation and ⁇ cking of the ligated paw.
  • Induced pain in the nerve-injured limb can be measured using Von Frey filaments, applied to the plantar surface of the paw to test for responses to non-noxious tactile stimulation.
  • the onset of this heightened mechanical allodynia is quite rapid and it persists for 2-3 months.
  • the CCI ligation was performed on the left sciatic nerve in three groups of rats. Von Frey testing of mechanical allodynia was performed on both paws on Day 14 to determine the analgesic efficacy of the test agent. The testing was performed pre-dosing, and at 30 and 90 minutes post-dosing.
  • Treatment groups The animals were allocated to treatment groups based on their baseline Von Frey data, measured prior to surgery. The mechanical allodynia scores for each group were reviewed to ensure that the mean values and standard deviation satisfied the assumption of homogeneity. Table 3 presents the treatment groups. Thirty-one (31) animals were used on the study. Thirty (30) animals were initially allocated to treatment groups, and the remaining four (4) animals were held as spares. One spare was then used to replace an animal that died during surgery. Body weights were taken one day after arrival, prior to surgery and weekly thereafter. On Day 14, after the final behavioral testing, the animals were euthanized by carbon dioxide asphyxiation. No necropsy was performed nor tissues collected.
  • the rats Prior to surgery, the rats were sedated using inhaled lsoflurane anesthetic. The left leg was shaved and prepped. The common sciatic nerve was exposed and freed from adherent tissue at mid-thigh by separating the muscle (biceps femoris) by blunt dissection. Proximal to the sciatic nerve's trifurcation; approximately 7 mm of nerve was freed from the adhering tissue.
  • Four ligatures approximately 1 mm apart, were tied loosely around the nerve using 6.0 chromic catgut. Each of the sutures was tied loosely with a square knot around the sciatic nerve. A brief twitch in the muscle surrounding the exposure was an indicator of the desired degree of constriction.
  • the filaments were pressed perpendicularly against the ventral surface of the paw until they bent and the rat responded by withdrawing its paw when this was considered painful. Threshold allodynia was determined using the Chaplan up-down method which provided the precise force for withdrawal for each rat using a psychophysical scale of testing. Both the left and right hindpaws were tested at each time point. The order of testing was the ipsilateral (affected) limb followed by the contralateral limb. There were approximately 20 minutes in between testing the two limbs.
  • mice in Groups 1 and 3 received an S. C. injection of either vehicle or (+)-naloxone HCI according to Table 3. They were dosed at 1 rnL/kg.
  • animals in Group 2 received an I. P. injection of Gabapentin according to Table 3. They were also dosed at 1 mL/kg.
  • FIG. 2A The data in FIG. 2A demonstrate a significant allodynia in the left paw for all three groups in relation to the pre-surgery baseline data (p ⁇ 0.01 for treatment, p ⁇ 0.001 for time). After dosing, both Gabapentin and ( ⁇ )-naioxone were effective at significantly reducing the allodynia at both 30 and 90 min.
  • the right paw data are depicted in FIG. 2B and these data reveal that there was no aliodynia in the right paw as expected in this model. There were no significant group differences or differences across time with the exception of the Gabapentin group, which
  • (+)-naIoxone to reduce allodynia in the left paw of animals that achieved neuropathic pain when tested on Day 14 post-surgery.
  • the results showed that significant analgesia was achieved by (+)-na!oxone at both timepoints tested, which was similar to the results of the positive control, Gabapentin.
  • (+)-naloxone-HCI was an effective analgesic for reversing neuropathic pain over a timecourse of 30 and 90 min post-dosing on Day 14 using a dose of 66.7 mg/kg.
  • Acetaminophen-induced liver damage is the most common cause of death due to acute liver failure.
  • Treatment with a (+)-morphinan may reduce or prevent acute liver injury induced by acetaminophen (APAP).
  • APAP acetaminophen
  • the effectiveness of the (+)-morphinan compounds disclosed herein to reduce liver injury after exposure to a toxic dose of APAP may be tested in mouse model of APAP-induced toxicity.
  • APAP-induced liver toxicity may be induced in male
  • mice At 12 hrs post-injection, all mice may be sacrificed and the following data collected a) liver histology with H&E staining, b) serum ALT (alanine
  • ( ⁇ )-morphinans may be tested in model animal systems.
  • the Adjuvant Induced Arthritis (AIA) Lewis rat model is good animal model for rheumatoid arthritis, a disease characterized by a T- lymphocyte and macrophage cellular infiltrate.
  • the rats may be divided into control and treatment groups ( ⁇ 10 rats/group) as indicated below.
  • the animals may be treated twice daily beginning on the day of injection of mycobacterium and continuing for 4 weeks.
  • Rats may be weighed twice a week during the course of the trial.
  • rats may be observed two-three times a week for clinical signs of AIA.
  • al! four paws may be examined and scored using the following scale for a maximum of 16 points.
  • (+)-morphinans to reduce inflammation may also be tested in the EAE (Experimental Autoimmune Encephalomyelitis (EAE) Lewis rat model.
  • EAE Extra Autoimmune Encephalomyelitis
  • female Lewis rats (7 weeks old; ⁇ 200 g) may be injected with 0.05 ml Mycobacterium tuberculosis in a guinea pig spina! cord emulsion into each main foot pad of the rear paws.
  • the rats may be divided into control and treatment groups (-10 rats/group) as indicated below.
  • the animals may be treated twice daily beginning on the day of injection of mycobacterium.
  • Rats may be weighed twice a week and, starting the second week, may be observed two-three times a week for clinical signs of EAE using the scoring system detailed below. Rats which are borderline in scores may be given a one half score, such as 3.5, Moribund rats will be euthanized.
  • mice Female nude mice (Hsd:Athymic Nude-Foxn1 nu/nu; 5-6 weeks old) may be injected sc into the right shoulder region with EGFR expressing human tumor cells. The mice may be divided into the following treatment groups (-10 rats/group):
  • Palpation for tumors may begin 7 days post implantation. Tumors may be observed and measured 3 times a week. Caliper measures - in mm width (smalt measure) x length (large measure). Body weight may be recorded on day 1 of the trial (day of cell implantation) and once weekly until necropsy. NIH euthanasia guidelines for rodent tumors will be followed (e.g., if tumor diameter exceeds 20 mm, if tumor is ulcerated tumor, if tumor severely restricts the animal's ability to eat, drink, eliminate wastes, breathe, or ambulate, or if animal is becoming emaciated and/or loses more than 20% of pre-study weight).

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