Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
  • C12P19/00—Preparation of compounds containing saccharide radicals
  • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• • C—CHEMISTRY; METALLURGY
  • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
  • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
  • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
  • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof

Definitions

• the invention relates to the field of microbial extraction, and the post-extraction method of special acyl gellan gum.
• Knotted gelatin can be said to be one of the most excellent thickeners and stabilizers at present, and has excellent condensing properties.
• Gellan gum gel is easy to use, has good flavor release, high thermal stability, easy to melt in the mouth, high transparency, time to earn and controllable, fiber is not easily affected by ⁇ , product is stable, and has various rot Hook characteristics, etc.
• Gellan gum is a kind of bacteria isolated from the natural screening of M.
• the molecular basic structure of gellan gum is a main chain composed of repeating tetrasaccharide units.
• the basic structure of gellan gum molecules is a main chain composed of repeating tetrasaccharide units.
• the monosaccharides involved are: glucose, 1 glucose ⁇ , 1 glucose and L-rhamnose.
• the glyceryl group is located at the 2-position of the lignin-bonded 3 ⁇ 43 ⁇ 4, and the B-face is at 6 ⁇ _h.
• the ruthenium is easily removed from the surface, so that the natural form of gellan gum is obtained in a deacylated form.
• the natural form of gellan gum can also be referred to as a high acyl product.
• High acyl gellan gum contains 11-13% glyceryl acyl group and 5% acetyl group, total acyl group content is between 15 ⁇ 18% (percent of dragon); low bismuth gellan gum has less than 1% content of glycerol art And less than 1% of the content of B ⁇ ' is 3 ⁇ 4% or less. «The group has a significant effect on the properties of the gel. High acyl type can produce a soft, elastic and non-brittle spirit, while low concealed cold glue produces a real, non-elastic, but very brittle enamel.
• Low-knot gelatin gel@4 is widely used in the field of 4k and tissue culture cranes, but detailed reports on the production process of low-cold gelatin glue, especially the low-acyl gellan gum production process used in the above-mentioned fields are rare.
• the low-acidification type gellan gum product contains a part of protein, the content is about 17% (by weight), so it is opaque, and the low acyl clear type gellan gum product adopts a filtering process to remove most of the insoluble matter.
• the gel thus formed is clear and transparent and has a very high transparency. Since the application of low acyl clear gellan gum is more common in the food industry and the like, in many common cases, gellan gum refers to a product with a low acyl clear type.
• the production process of the low acyl clear type gellan gum is inconvenient that it is difficult to completely remove the protein in the colloid, and the product is affected by a certain degree, generally only 80% or more; at the same time, the low «3 ⁇ 4 type gellan gum contained in the product contains Part of the divalent metal cation makes the gellan gum product difficult to dissolve, and the formed condensation is white, which affects the quality of the product.
• the flocculation stage uses a divalent or multivalent alkali metal salt to flocculate the gellan gum product in a high acyl state, while the high acyl gellan gum is a divalent or polyvalent alkali metal.
• the sensitivity of the salt is much less than that of the low acyl gellan gum, so the efficiency of flocculation is not high and the product yield is affected.
• the earning agents in the tissue culture medium are generally deleted agar.
• impurities in agar and hindrance factors affect plant tissue growth, so low-salt-type gellan gum products are a promising agar substitute.
• the gellan gum is very high, and the 3 ⁇ 43 ⁇ 4: ⁇ production, the quality of the product is more stable than the agar extracted from seaweed.
• the gel time is shorter than that of the agar, which saves time; at the same time, the knot is cold! 3 ⁇ 4 is very stable under high temperature conditions, and the legs are combined with thermophilic microorganisms.
• IDlk's food-grade low-awake cold gel can be used in tissue culture media, but it is made in China compared to the US Gynecco GELZANTMCM model gellan gum and Sigma Phytagel gellan gum. There are still many problems in the application of food-grade gellan gum in the medium. The specific performance is as follows:
• the food grade low acyl gellan gum has poor solubility, poor dispersibility, easy to clump, and the hydration temperature needs to be above 80 °C.
• the medium type of the culture medium has good dispersibility of cold gel and hydrates at 60° (about.
• the food-grade gellan gum should be strictly adhered to the order of dissolving the colloid and then adding the metal ions. If the gellan gum powder and the metal salt are mixed and then dissolved, the solution will often be difficult to produce. A large amount of milky white floc. The foreign medium type gellan gum does not have this problem.
• the general food grade gellan gum strength is above 900 ⁇ cm 2
• the foreign medium type gellan gum strength is between 40 (K600 g/cm 2 ; at the same time, the general food grade gellan gum has a transmittance of about 85%.
• the foreign medium model is more than 90% cold.
• the object of the present invention is to provide a new method for producing a low acyl gellan gum, and a further object is to produce a new low acyl acid. Knotted gelatin and a new method for the use of low-tangling gellan gum for tissue culture.
• the method for extracting low acyl clear gellan gum comprises deacylation treatment of a gellan gum fermentation broth, enzymatic treatment, bivalent or polyvalent metal cation flocculation low acyl gellan gum, clarification treatment of gellan gum solution, The step of dehydrating the gellan gum solution, removing the divalent or multivalent cations and decolorizing and drying and pulverizing, preferably, it is also possible to formulate a suitable mass of chelation system before the dry pulverization step to chelate in the gellan gum. Additional divalent cations may be added during the process while maintaining a relatively stable pH.
• the specific steps are:
• Adding different enzyme preparations in the surface of the step (1) to remove the insoluble impurities and bacterial fragments in the fertile seed more specifically, adding an enzyme preparation to keep the enzyme hydrolyzed, and then adding another Enzyme incubation, and so on;
• step (2) after the enzymatic treatment, adding water-soluble flocculation, adjusting the pH to alkaline, and separating the solid and liquid to remove most of the water and the pigment in the fermentation liquid to obtain a concentrated gellan gum;
• the crude gellan gum obtained in the step (3) is subjected to 3 ⁇ clarification treatment to obtain a clarified low «gellan gum solution;
• the gellan gum obtained in the step (5) is chopped into fine particles, and the S3! ion «process ⁇ 3 ⁇ 4 ⁇ process removes most of the divalent cations or polyvalent cations in the gellan gum; the gellan gum which is slightly dried and dried Soak with a lower alcohol and » filter to achieve complete bleaching
• the gellan gum solid material obtained in the step (6) is dried and pulverized to obtain a highly transparent low acyl clear gellan gum product.
• steps (6) and (7) further comprise the following steps:
• the gellan gum obtained in the step (6) is formulated with a suitable mass of the chelate compound system to sequester the divalent cation which may be additionally added during the use of the gellan gum while maintaining the pH value relatively stable and stirring uniformly.
• the cellulase, the vinease and the protease which have been dissolved in a small amount of water and dissolved in different concentrations are added to the faint obtained in the step (1), and maintained at different times.
• the protease is a neutral protease or an alkaline protease.
• the material in the step (2) is cooled to below 35 , and the bivalent or polyvalent water-soluble alkali metal salt is added to flocculate the low «cold gel, and the alkali is added to adjust the pH, and the solid-liquid separation is carried out by centrifugation.
• Dissolve the material prepared in step (3) dissolve it with 1 ( ⁇ 20 times the amount of deionized water, add acid to adjust the pH to neutrality, heat up to 85 0. C, fully earn the face completely dissolved; delete
• the plate frame is filtered, filtered by high-speed centrifugation or microporous membrane filtration, and the deacylated gellan gum solution obtained in the step (3) is clarified, and the clarification treatment should be above 65 to prevent the solution from forming a gel, and the obtained clarification is obtained.
• the transmittance of the solution should be above 92%.
• the metal cation salt of AS as the mass is added, and the formed condensation press is dehydrated to obtain a low acyl clear type gellan gum block or film having a water content of about 80%. .
• the low acyl gellan gum obtained by the step (5) with a water content of about 80% is chopped into small particles, and is put into a 3 to 5 times mass of Aig added to the water of one price, soaked and high 3 ⁇ 4», to the ion Exchange means to convert the colloid from the divalent cation salt form to the monovalent cation salt form; 3 ⁇ 4 3 ⁇ 4 ⁇ to 3-5 times the mass of water added to the appropriate chelating agent, soaked and stirred at high speed to remove most of the divalent in the colloid Or multivalent cations.
• the gelatinized lyophilized fox squeezing is lightly immersed in the liquid with a low acidity of 2 times the mass, soaked and quickly earned, and then filtered to completely remove the pigment by 3 l4m.
• the product obtained in the step (6) is dried at 75 to 80 ° C, and pulverized to make a 95% leg 80 mesh screen, and the obtained low-salt type gellan gum product.
• steps (6) and (7) further comprise the following steps:
• the gellan gum obtained in the step (6) is formulated with a suitable mass chelation clock system to sequester the divalent cations which may be additionally added during the use of the gellan gum while maintaining the relative stability of the P H value.
• the water in the cold glue « is about 80%, and the mixture is evenly mixed with the gellan gum.
• the agent and the acid may be mixed first and then added, or may be added in order.
• the antioxidant added in the step 1 may be, but not limited to, one or more of ascorbic acid, iso VC sodium, pyrosulfide, potassium pyrosulfite, potassium hydrogen sulfate, and cysteine.
• the concentration of the added antioxidant is preferably from 100 to 300 ppm, more preferably 15 (250 ppm).
• the ⁇ for adjusting the pH added in the step 1 is not limited to one or more of NaOH, OH, N3 ⁇ 4C0 3 , K 2 C0 3 , preferably NaOH, KOH, and more preferably NaOH.
• step 1 pH is adjusted to within the range of 9.5 to 11, and a better pH is around 10.
• the base for adjusting the pH should be first formulated into a solution having a concentration of 10%.
• step 1 maintain between 85 °C, and better between 8 88 °C.
• step 1 keep the time 1 (5 m, better «time is around 10).
• the acid which adjusts the pH may be an inorganic acid or an organic acid.
• the inorganic acid includes, but is not limited to, one or more of hydrochloric acid, sulfuric acid and phosphoric acid; and the organic acid may be, but not limited to, formic acid, acetic acid, citric acid, malic acid or tartaric acid.
• the acid preferably used is a mineral acid, more preferably hydrochloric acid. The amount of acid used is such that the pH of the fermentation broth system can be adjusted to about 7.
• step 1 adjust the pH of the solution with 10% of the first female.
• the enzymatic conditions in the step 2 are respectively: the cellulase concentration is preferably 50 (2000 ppm, more preferably 1000 to 1500 ppm; the enzymatic hydrolysis time is preferably 4 to 8 hours, more preferably 5 to 6 hours).
• the enzyme 3 ⁇ 4® is preferably 40 to 50 ° C, more preferably 43 5 ° C.
• the lysozyme concentration is preferably 5 (K500 ppm, more preferably 10 (200 ppm ; preferably the enzymatic hydrolysis time is 2 to 4 hours, more preferably 2.5 to 3.5 hours; the enzyme is preferably 3 (0 ° C, more preferably 35 to 37 ° C.
• the protease concentration is preferably 100 to 1000 ppm, more preferably 300 to 500 ppm (for tedious); the enzymatic hydrolysis time is preferably from 1 to 5 hours, more preferably from 2 to 3 hours; the enzyme «3 ⁇ 4 is preferably 3 (M0'C, more preferably 3 (35°) C. wherein the protease is a neutral protease or an alkaline protease.
• the water-soluble salts used in the step 3 include, but are not limited to, magnesium, calcium, barium, zinc, and water-soluble.
• the amount of the water-soluble alkali to be used in the step 3 preferably accounts for 0.1% by weight of the hair, more preferably 0.3% to 0.4%.
• the base used in the step 3 includes, but is not limited to, one or more of KOH, NaOH, Na 2 C0 3 , NaHC0 3 , ⁇ & 3 ⁇ 0 4 .
• the solid-liquid separation equipment used in step 3 can be selected but not limited to the box type polypropylene plate frame filter press. Bag press, polypropylene plate is a dragon.
• the length of the gellan gum fiber after the breakage in step 4 is not /Mil 10 j , and the water volume in the township is about 80%.
• Step 4 The dispersed gellan gum is dissolved in 10 to 20 times the amount of deionized water, more preferably 15 to 20 times as deionized water.
• the solution is heated to a temperature of 80 to 95 t, more preferably 85 to 90 °C.
• the clarification equipment can be used, but not limited to, plate frame J E filter, high speed centrifugation or microporous filter ' filter, crane frame shed Stffi filter.
• step 4 clarify the m3 ⁇ 43 ⁇ 43 ⁇ 4 65 °C or more during the treatment to prevent the formation of the solution, and the better temperature is about 75 °C.
• the metal salt added when forming the gel includes, but is not limited to, a soluble monovalent alkali metal salt (one or more of potassium chloride, sodium chloride, potassium sulfate, sodium sulfate), a divalent alkali metal salt.
• a soluble monovalent alkali metal salt one or more of potassium chloride, sodium chloride, potassium sulfate, sodium sulfate
• a divalent alkali metal salt a soluble monovalent alkali metal salt
• Combinations of (calcium chloride, magnesium chloride) and polyvalent (ferric chloride) salts, female monovalent and bivalent are considered factors, and can be more erected.
• the monovalent alkali salt added when the gel is formed is 0.8.2% by weight of the clear gum, and the divalent metal salt added is 0.05 ⁇ 0.1% by weight of the clear gum.
• step 5 a soluble 'genus that is added at the time of earning is formed into a 30% concentration solution.
• the solid-liquid separation equipment used in step 5 can be selected but not limited to the box type polypropylene plate frame filter press, 13 ⁇ 4M polypropylene plate ship.
• step 6 the glue cutter is cut, and the low-occlusion cold glue is chopped into small columnar particles, the particle diameter is less than 3 3 ⁇ 4, and the length is less than 12 mm.
• the monovalent metal cation used in the step 6 includes, but is not limited to, a soluble monovalent alkali metal salt (one or more of potassium chloride, sodium chloride, potassium sulfate, and sodium sulfate), and the amount is preferably in a solution.
• the concentration reaches 500 (10,000 ppm, more preferably 600 (8000 ppm o)
• the agent used in the step ⁇ may be, but not limited to, one or more of sodium citrate, tripotassium citrate, sodium hexametaphosphate, potassium hexametaphosphate, sodium pyrophosphate, and potassium polyphosphate.
• the preferred chelating agents are sodium citrate and sodium hexametaphosphate, more preferably sodium citrate.
• the amount of chelating agent added is preferably the concentration in the solution.
• the press equipment in step 6 is made of cloth «pressing machine.
• the lower alcohol used in the step 6 may be one or more of ethanol, isopropanol and n-butanol, preferably ethanol and isopropanol, more preferably isopropanol.
• the amount used is preferably 2 times the weight of the gellan gum wet particles, more preferably 2.5-3.5 times.
• the chelating agent in step 6' includes, but is not limited to, one or more of 1 «, tripotassium monoxide, hexametaphosphate, potassium hexametaphosphate, sodium pyrophosphate, and potassium polyphosphate.
• the preferred chelating agent is a phosphate salt, more preferably hexameta.
• the acid used in the step 6' may be an inorganic acid or an organic acid.
• the inorganic acid may be, but not limited to, one or more of hydrochloric acid, sulfuric acid or phosphoric acid; the organic acid may not be limited to one or more of formic acid, acetic acid, montanic acid, malic acid or wine.
• the acid preferably used is an organic acid, more preferably citric acid.
• the amount of chelating agent used in step 6' is 9-10% (mass%) in the final dry product, and the acid used is 0.5-1% (mass%) in the final dried product.
• the drying equipment used in step 7 may be, but not limited to, vacuum drying and boiling drying, the temperature is controlled between 75 and 80 ° C, and the time is controlled between 1 and 1.5 hours.
• Example 1 Fascia entanglement treatment
• the filter cake obtained from C was first broken up into short fibers by a dispersing machine, deionized water was added in an amount of 15 times by weight, and the solution was heated to raise it by i3 ⁇ 4 90 °C.
• the photometer measures the transmittance greater than 92%. After clarification, the solution is connected to the tank.
• the clarified gellan gum solution obtained from D was maintained at a temperature of 65 Torr or more to prevent gel formation, and then a solution of 400% of a potassium chloride solution of 30% was added to the solution, and the temperature was slowed down to 5 and then forced to drop lj'50.
• a solution of 400% of a potassium chloride solution of 30% was added to the solution, and the temperature was slowed down to 5 and then forced to drop lj'50.
• the colloid of the more brittle gel is pressed with a box-type polypropylene plate frame filter press to obtain a gel-free film or a 500 kg of gelatinous film with a moisture content of about 80%.
• the gellan gum block or film obtained from E is chopped into small columnar particles by a rubber cutter with a particle diameter of less than 3 mm and a length of less than 12 3 ⁇ 4.
• Add cold gelatin small particles SA to 3 times the mass of deionized water, add potassium hexametaphosphate to make the degree reach 5000ppm, slowly »solution 10 and filter with filter cloth; slag residue and then soak with 2.5 times ethanol solution and quickly Stir for 30 minutes; remove the ethanol solution with a bag press to obtain 495 kg of wet gellan gum loam.
• the 10m 3 gellan gum fermentation liquid is heated to 90 ° C, and the iso-VC sodium is added to the concentration of 200 ppm under stirring; then the 10% concentration of NaOH is added to adjust the pH to 10.0, at 90 ° C ⁇ cattle Stir slowly under 10 and then add 10% acetic acid, adjust the pH to 7.0, and obtain the next step.
• the filter cake obtained from C was first broken up into short fibers by a dispersing machine, deionized water was added in an amount of 15 times by weight, and the solution was heated to raise it by 90 °C.
• the gellan gum solution was circulated and filtered by a microporous membrane to obtain a clarified gellanine gel fractionation spectrophotometer with a luminosity of more than 92%. After clarification, the solution was connected to the fiber tank.
• the clarified gellan gum solution obtained from D is maintained at 65 ⁇ or more to prevent the formation of the formation, and then a solution of 400% of a 30% sodium chloride solution is added to the solution, and the temperature is slowly lowered by 5 to 1 501 501.
• the colloidal gel of the brittle gel is pressed with a box-shaped polypropylene plate frame to obtain a gellan film or a block of 500 kg having a water content of about 80%.
• the product obtained from F was dried at 75 ° C and pulverized to a 95% Mil 80 mesh screen.
• the obtained low-grade clear gelatin glue product is 100kg.
• the filter cake obtained from C was first broken up into short fibers by a dispersing machine, deionized water was added in an amount of 15 times by weight, and the solution was heated to raise the temperature to 90 °C. Add appropriate amount of diatomaceous earth to the solution, stir evenly, and control the temperature at 85 °C. Use the pre-coated diatomaceous earth filter aid to form a propylene cycle to filter the gellan gum solution, and obtain the clarified gellan gum. ⁇ 3 ⁇ 4 photometer measures the transmittance greater than 92%, after clarification, the solution is connected to the tank.
• the clarified gellan gum solution obtained from D is maintained at a temperature above 65 °C to prevent gel formation. Then add 30% chlorination to the solution! 3 ⁇ 4 solution 25L, slow down 1S» 5 ⁇ and then force down 3 ⁇ 4 50 Below °C.
• the shape of the j «g brittle gel is pressed by the box-shaped polypropylene plate.
• the squeezing method is to obtain a gel-free film or gel 500kg with a water content of about 80%.
• the gellan gum block or film obtained by E is chopped into small columnar particles by a rubber cutter, and the particle diameter is less than 3 mm. The length is less than 12 mm.
• the product obtained from F was passed through a fluent dryer, dried at 75, and pulverized to a 95% ⁇ 80 mesh screen.
• the obtained low acyl clear gellan gum product was 100 kg.
• the quality of the products has been greatly improved and reached the advanced level in foreign countries.
• the product has a good view, high transparency, and high product gel strength. Specifically, the chromaticity is greater than 83%, the transmittance is above 85%, and the gel strength is greater than 1000 g/cm 2 .
• the 10m 3 gellan gum fermentation liquid is heated to 90 ° C, and the ascorbic acid is added to the concentration of 150 ppm in the stirring state; the pH is adjusted to 10.0 by adding 10% concentration of KOH, at 90 ° C. Slow mixing 10 Minutes; add 10% hydrochloric acid, adjust the pH to 7.0, and obtain the next step.
• the filter cake obtained from C was first broken into short fibers by a dispersing machine, deionized water was added in an amount of 15 times by weight, and the solution was heated to raise it by 90. Add AiS amount of silicon to the solution, and evenly control it at 85 °C. Circulating and filtering the gellan gum solution with a pre-coated diatomaceous earth filter aid box-type polypropylene 3 ⁇ 4 plate filter press, 3 obtained clarified gellan gum The dissolution is determined by the photometer to be greater than 92%, and after clarification, the solution is connected to the tank.
• the temperature of the clarified gellan gum solution obtained from D should be maintained above 65 °C to prevent the formation of gel. Then add 400L of 30% potassium chloride solution to the solution, and slowly drop to >50° ⁇ after »5. . Shape ⁇ More brittle «The colloid is pressed with a box of polypropylene frame to obtain a cold water of about 80% of the moisture «Piece 500kg.
• the gellan gum-cutting machine obtained by E cuts off small particles with a particle diameter of less than 3 and a length of less than 12 3 ⁇ 4.
• the product obtained from G was passed through an il STP dryer and dried at 75 and pulverized to pass 95% through an 80 mesh screen.
• the obtained low-clear clear gellan gum product is 110kg.
• the 10m 3 gellan gum is heated to 90 ° C, and the iso-VC sodium is added to the concentration of 200 ppm under stirring; then the 10% concentration of NaOH is added to adjust the pH to 10.0, and slowly under 90 ⁇ ⁇ Stir for 10 minutes; add 10% strength acetic acid, adjust the pH to 7.0, and the resulting solution goes to the next step.
• the temperature of the clarified gellan gum solution obtained from D should be maintained above 65'C to prevent gel formation. Then add 30L of 30% sodium chloride solution to the solution, and slowly drop to >50° ⁇ after »5. . Shape / 3 ⁇ 4 ⁇ More brittle gel colloids are pressed with a box-shaped polypropylene plate frame filter to obtain 500kg of gellan film or glue with a moisture content of about 80%.
• the gellan gum block or film obtained from E is chopped into small columnar particles by a rubber cutter with a particle diameter of less than 3 mm and a length of less than 12 3 ⁇ 4*.
• G. fiAS is the quality of the "Q" system
• the product obtained from G was boiled in a dryer, dried at 75 ° C, and pulverized, and passed 95% through an 80 mesh screen.
• the obtained low ⁇ 3 ⁇ 4 type gellan gum product is 110kg.
• the 10m 3 gellan gum fermentation liquid is heated to 90 ° C, and the potassium metabisulfite is added to the stirring state.
• the concentration was 250 ppm; 10% concentration of KOH was added to adjust the pH to 10.0, and the mixture was slowly stirred at 90 ° C for 10 minutes; 10% strength of citric acid was added to adjust the pH to 7.0, and the resulting solution was passed to the next step.
• the filter cake obtained from C is first broken down into short fibers by a blow, and the deionized water is poured into the mouth 15 times of the dragon, and the hot solution is heated to 90 °C.
• Add appropriate amount of diatomaceous earth to the solution stir evenly, control at 85 °C, circulate and filter the gellan gum solution with a box-type polypropylene plate filter press precoated with diatomaceous earth filter aid, 3 ⁇ 4 to obtain clear and cold
• the peptance photometer measures the transmittance greater than 92%, and after clarification, the solution is connected to the tank.
• the temperature of the clarified gellan gum solution obtained from D was maintained above 65 °C to prevent gel formation, and then 25 L of a 30% calcium chloride solution was added to the solution, and the temperature was slowly lowered to 5 °C and below 50 °C.
• the gel of the more brittle gel is pressed with a box-type polypropylene plate frame filter fLEE to obtain a gel-free film or gel 500 kg with a water content of about 80%.
• the gellan gum block or film obtained by E is chopped into small columnar particles by a rubber cutter, and the particle diameter is less than 3 mm. The length is less than 12 «*.
• Add cold gelatin granules & mash to 3 times the mass of deionized water, add sulfur to achieve SOOOppm, slow »solution 10 and filter with filter cloth; filter residue and then soak with 2.5 times of B earn and 'win 30 Use a cloth squeezer to remove the B earning liquid, and get 495kg of wet gelatinized pine.
• the product obtained from G was dried by a vacuum dryer at 75 ° C and pulverized, and passed 95% through an 80 mesh screen.
• the obtained low acyl clear gellan gum product was 108 kg.
• the gellan gum has good dispersibility, and the hydration is about 6CTC. It dissolves at a lower level and prepares a gel with extremely high transparency.
• the quality of the product has been greatly improved.
• the product is good, and the product is high.
• the chromaticity is greater than 83%, the transmittance is above 90%, and the gel strength is between 40 (650 g/cm 2 ).

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