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WO2010076305A1 - Process for cell-free production of chemicals - Google Patents

Process for cell-free production of chemicals Download PDF

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Publication number
WO2010076305A1
WO2010076305A1 PCT/EP2009/067954 EP2009067954W WO2010076305A1 WO 2010076305 A1 WO2010076305 A1 WO 2010076305A1 EP 2009067954 W EP2009067954 W EP 2009067954W WO 2010076305 A1 WO2010076305 A1 WO 2010076305A1
Authority
WO
WIPO (PCT)
Prior art keywords
ec
process
glucose
enzymes
pyruvate
Prior art date
Application number
PCT/EP2009/067954
Other languages
English (en)
French (fr)
Inventor
Andre Koltermann
Ulrich Kettling
Volker Sieber
Original Assignee
Süd-Chemie AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP20080022558 priority Critical patent/EP2204453B1/en
Priority to EP08022558.4 priority
Application filed by Süd-Chemie AG filed Critical Süd-Chemie AG
Publication of WO2010076305A1 publication Critical patent/WO2010076305A1/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16Butanols
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels
    • Y02E50/17Grain bio-ethanol
PCT/EP2009/067954 2008-12-30 2009-12-28 Process for cell-free production of chemicals WO2010076305A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP20080022558 EP2204453B1 (en) 2008-12-30 2008-12-30 Process for cell-free production of chemicals
EP08022558.4 2008-12-30

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
KR20117017289A KR101328382B1 (ko) 2008-12-30 2009-12-28 화합물의 무세포 제조를 위한 방법
BRPI0923822-0A BRPI0923822A2 (pt) 2008-12-30 2009-12-28 Processo para a produção livre de células de produtos químicos
EP09798943.8A EP2798070A1 (en) 2008-12-30 2009-12-28 Process for cell-free production of chemicals
CN200980153487.4A CN102272314B (zh) 2008-12-30 2009-12-28 无细胞生产化学品的方法
JP2011544040A JP5461582B2 (ja) 2008-12-30 2009-12-28 無細胞による化学物質の生産方法
MX2011006745A MX2011006745A (es) 2008-12-30 2009-12-28 Procedimiento para produccion sin celulas de compuestos quimicos.
CA2746861A CA2746861C (en) 2008-12-30 2009-12-28 Process for cell-free production of chemicals
US13/143,019 US8859247B2 (en) 2008-12-30 2009-12-28 Process for cell-free production of chemicals

Publications (1)

Publication Number Publication Date
WO2010076305A1 true WO2010076305A1 (en) 2010-07-08

Family

ID=40651914

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/067954 WO2010076305A1 (en) 2008-12-30 2009-12-28 Process for cell-free production of chemicals

Country Status (10)

Country Link
US (1) US8859247B2 (un)
EP (2) EP2204453B1 (un)
JP (1) JP5461582B2 (un)
KR (1) KR101328382B1 (un)
CN (1) CN102272314B (un)
BR (1) BRPI0923822A2 (un)
CA (1) CA2746861C (un)
DK (1) DK2204453T3 (un)
MX (1) MX2011006745A (un)
WO (1) WO2010076305A1 (un)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010077806A1 (en) 2008-12-15 2010-07-08 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways
CA2797786A1 (en) 2010-05-07 2011-11-10 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through enzyme relocation
KR20130122729A (ko) 2010-08-31 2013-11-08 그린라이트 바이오사이언시스, 아이엔씨. 프로테아제 조작을 통한 대사 경로에서 유동의 제어 방법
WO2013020022A2 (en) * 2011-08-04 2013-02-07 St. Louis University Methods and compositions for decomposition of biomass
SG2014014377A (en) 2011-09-09 2014-05-29 Greenlight Biosciences Inc Cell-free preparation of carbapenems
EP2700714A1 (en) 2012-08-20 2014-02-26 Clariant Produkte (Deutschland) GmbH Cell-free and minimized metabolic reaction cascades for the production of chemicals
US9512408B2 (en) 2012-09-26 2016-12-06 Butamax Advanced Biofuels Llc Polypeptides with ketol-acid reductoisomerase activity
EP2935598A1 (en) 2012-12-21 2015-10-28 Greenlight Biosciences Inc. Cell-free system for converting methane into fuel, pyruvate or isobutanol
WO2014182054A1 (ko) * 2013-05-07 2014-11-13 고려대학교 산학협력단 3,6-안하이드로-l-갈락토오스를 대사하는 재조합 미생물 및 이의 용도
CA2919383A1 (en) 2013-08-05 2015-02-12 Greenlight Biosciences, Inc. Engineered proteins with a protease cleavage site
EP3180437A2 (en) * 2014-08-14 2017-06-21 Repsol, S.A. Process for production of branched chain alcohols
CN107208117A (zh) * 2014-12-16 2017-09-26 新派克股份公司 异丁醇生产的酶促方法
US20180273985A1 (en) * 2015-03-19 2018-09-27 William Jeremy Blake Cell-free production of butanol
CN105274041A (zh) * 2015-10-28 2016-01-27 山东大学 一株重组大肠杆菌及其在生产2-丁醇中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008006038A2 (en) 2006-07-06 2008-01-10 Integrated Genomics, Inc. Methods and compositions for butanol production
WO2008052596A1 (en) 2006-10-31 2008-05-08 Metabolic Explorer Process for the biological production of n-butanol with high yield

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008006038A2 (en) 2006-07-06 2008-01-10 Integrated Genomics, Inc. Methods and compositions for butanol production
WO2008052596A1 (en) 2006-10-31 2008-05-08 Metabolic Explorer Process for the biological production of n-butanol with high yield

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
ALGAR E M ET AL: "STUDIES ON CELL-FREE METABOLISM ETHANOL PRODUCTION BY EXTRACTS OF ZYMOMONAS-MOBILIS", JOURNAL OF BIOTECHNOLOGY, vol. 2, no. 5, 1985, pages 275 - 288, XP023944442, ISSN: 0168-1656 *
ALGAR, E.M.; SCOPES, R.K., JOURNAL OF BIOTECHNOLOGY, vol. 2, no. 5, 1985, pages 275 - 287
ALLAIN ERIC J: "Cell-free ethanol production: the future of fuel ethanol?", JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol. 82, no. 2, February 2007 (2007-02-01), pages 117 - 120, XP002531755, ISSN: 0268-2575 *
EZEJI ET AL: "Bioproduction of butanol from biomass: from genes to bioreactors", CURRENT OPINION IN BIOTECHNOLOGY, vol. 18, no. 3, 8 June 2007 (2007-06-08), LONDON, GB, pages 220 - 227, XP022110184, ISSN: 0958-1669 *
LAMBLE HENRY J ET AL: "Metabolic pathway promiscuity in the Archaeon Sulfolobus solfataricus revealed by studies on glucose dehydrogenase and 2-keto-3-deoxygluconate aldolase.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 36, 5 September 2003 (2003-09-05), pages 34066 - 34072, XP002531754, ISSN: 0021-9258 *
LAMBLE, H.J.; HEYER, N.L.; BULL, S.D.; HOUGH, D.W.; DANSON M.J., J. BIOL.CHEM., vol. 36, no. 5, 2003, pages 34066 - 72
RICHTER F.W. (ED.): "Biotechnology: Research, Technology and Applications", 2008, NOVA SCIENCE PUBLISHERS, INC, NEW YORK, ISBN: 9781604569018, XP002531756 *
WELCH P ET AL: "STUDIES ON CELL-FREE METABOLISM ETHANOL PRODUCTION BY A YEAST GLYCOLYTIC SYSTEM RECONSTITUTED FROM PURIFIED ENZYMES", JOURNAL OF BIOTECHNOLOGY, vol. 2, no. 5, 1985, pages 257 - 274, XP023944441, ISSN: 0168-1656 *
WELCH, P; SCOPES R.K, JOURNAL OF BIOTECHNOLOGY, vol. 2, no. 5, 1985, pages 257 - 273
ZHANG ET AL., WELCH AND SCOPES, 1985
ZHANG Y.-H.P.; EVANS B.R.; MIELENZ J.R.; HOPKINS R.C.; ADAMS M.W.W., PLOS ONE, vol. 2, no. 5, 2007, pages E456
ZHANG Y.H.P.; YE, X.; WANG, Y., BIOTECHNOLOGY: RESEARCH, TECHNOLOGY AND APPLICATIONS, 2008

Also Published As

Publication number Publication date
EP2798070A1 (en) 2014-11-05
US8859247B2 (en) 2014-10-14
EP2204453A1 (en) 2010-07-07
KR20110101221A (ko) 2011-09-15
DK2204453T3 (da) 2013-06-10
MX2011006745A (es) 2011-10-17
BRPI0923822A2 (pt) 2015-07-14
CA2746861C (en) 2014-09-16
CA2746861A1 (en) 2010-07-08
CN102272314A (zh) 2011-12-07
JP2012513759A (ja) 2012-06-21
CN102272314B (zh) 2015-08-26
KR101328382B1 (ko) 2013-11-13
EP2204453B1 (en) 2013-03-13
JP5461582B2 (ja) 2014-04-02
US20110312052A1 (en) 2011-12-22

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