WO2010060362A1

WO2010060362A1 - Dressing for accelerating wound healing and method of use and manufacture thereof

Info

Publication number: WO2010060362A1
Application number: PCT/CN2009/075030
Authority: WO
Inventors: Rongxiang Xu
Original assignee: Rongxiang Xu
Priority date: 2008-11-26
Filing date: 2009-11-19
Publication date: 2010-06-03

Abstract

A wound dressing is provided for treating various kinds of wounds, including acute and chronic wounds in an animal such an human. The wound dressing comprises a reticular fabric such as non-woven spunlace or needle-felt fabric that is impregnated with a mixture of edible oil and beeswax, and has a net- in-net microstructure at ambient temperature.

Description

DRESSING FOR ACCELERATING WOUND HEALING AND METHOD OF USE AND

MANUFACTURE THEREOF

FIELD OF THE INVENTION

[0001] The invention relates to structured wound dressings, especially dressings for accelerating wound healing, and the use and manufacturing process thereof.

BACKGROUND OF THE INVENTION

[0002] Wound healing is a series of complex yet orderly biological processes that, when part or all of skin layer or other tissues is lost or damaged due to trauma or other injuries, partial tissues repair and restore the lost or damaged tissues through regeneration and reestablishment. The biological process mainly includes inflammatory response, cell proliferation/ formation of connective tissues, wound contraction and remodeling. Formation and existence of wound surface have a great impact on the functions of a part of or even the whole body. Thus, there exists important scientific problems that must be solved in the study of wound healing and wound treatment: how to accelerate wound healing and complete restoration of the lost and damaged tissues in the shortest period of time; and how to ensure the healed tissues having complete or close to complete restoration of the original tissue structure so as to ensure the restoration of the functions.

[0003] Conventionally a dressing is mainly applied to cover the wound surface so as to treat the wounds. Theoretically, dressings, as a barrier, can prevent the wound surface from microbial contamination. The idea behind this design is aimed to absorb and remove exudates of the wound surface and to isolate the wound surface from the environment. Therefore, the considerations in the design of wound dressings are mainly in the properties of biological inertness, non-toxicity and biocompatibility. However, such a structure of wound dressings results in a much drier environment in wound surface, which causes dehydration and scabs while the latter significantly hinders epithelialization of the wound.

[0004] Various wound dressings have been developed that contain polymeric films, hydrogels and hydrocolloids, which are used to manage wounds, but found to contain limitations in the ability to promote wound healing and skin regeneration so as to minimize scar formation and retain the normal physiological structure and function of the human body. SUMMARY OF THE INVENTION

[0005] One aspect of the present invention comprises a dressing, comprising: a reticular fabric impregnated with a mixture of edible oil and beeswax, and having a net-in-net micro structure at ambient temperature. Another aspect of the present invention comprises a kit, comprising: a dressing comprising a reticular fabric impregnated with a mixture of edible oil and beeswax, and having a net-in-net microstructure at ambient temperature. Still another aspect of the invention comprises a method for treating a surface wound of an animal, comprising: topically applying a dressing to cover the wound of the animal, wherein the dressing comprises a reticular fabric impregnated with a mixture of edible oil and beeswax, and having a net-in-net microstructure at ambient temperature. And still another aspect of the invention comprises a method of preparing a dressing, comprising the steps of: mixing a piece of reticular fabric with a mixture of edible oil or oil extract of botanical and beeswax; heating the mixture of the fabric and the oil-beeswax mixture to a temperature of about 70-130⁰C such that the oil-beeswax mixture becomes fluidic; taking out the fabric impregnated with the fluidic oil-beeswax mixture; and cooling the fabric impregnated with the oil-beeswax mixture at a temperature below 35⁰C.

INCORPORATION BY REFERENCE

[0006] All publications, patents, and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication, patent, or patent application was specifically and individually indicated to be incorporated by reference.

BRIEF DESCRIPTION OF THE DRAWINGS

[0007] The novel features of the invention are set forth with particularity in the appended claims. A better understanding of the features and advantages of the present invention will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the invention are utilized, and the accompanying drawings of which:

[0008] Figure 1 shows representative microstructures of non-woven fabric impregnated with a sesame- oil-and-beeswax mixture under a microscope (magnitude: 200X). A: An ideal net- in-net microstructure formed when the concentration of beeswax in the mixture of sesame oil and beeswax is about 7-8%. B: When the concentration of beeswax in the mixture of sesame oil and beeswax is above 10%, a net-in-net microstructure is nearly invisible as beeswax clogs up the holes in the fabric.

[0009] Figure 2 shows a female patient injured by earthquake and treated with an embodiment of the inventive wound dressing. A: Patient's right temple was injured during an earthquake with a bone-exposed open wound. B: Patient treated with the wound dressing applied to the wound. C: The wound dressing was affixed to her temple via a breathable adhesive pad. D: Patient's wound healed with re "g&e-neration of subcutaneous tissues and skin without obvious scar.

DETAILED DESCRIPTION OF THE INVENTION

[0010] One aspect of the present invention provides innovative wound dressings to solve the problems associated with traditional dressings and to provide highly effective means for regeneration of the human skin and the tissues beneath the skin in situ.

[0011] As described in detail below, the inventive wound dressing is comprised a construction design which adopts a net-within-net microstructure at ambient temperature which is breathable and allows efficient metabolism of the tissues at the wound site, maintains a physiologically moist wound environment, and promotes regeneration of new skin and the tissues beneath the skin including nerves, blood vessels, glands, soft tissues, muscles and connective tissues, thereby accelerating wound healing, restoring physiological structure and function of the body, and minimizing scarring and disablement.

[0012] The dressing can be used conveniently to cover the wound to accelerate wound healing. The wound healing is accelerated presumably, by activating and sustaining the intrinsic regenerative potential of the skin and the tissues beneath the skin and cultivating epidermal stem cells in situ and in vivo. The dressing can also protect the wound from infection and contamination, as well as allows outpatient change of wound dressing and longer intervals between each change of the dressing.

[0013] Another aspect of the invention in general pertains to a dressing that contains a unique net- within-net microstructure that is formed by a specific type of fabric impregnated with an oil-and- beeswax mixture at optimum concentrations of the oil and beeswax.

[0014] Still another aspect of the invention comprises a reticular fabric that is preferably non-woven spunlace or needle-felt fabric that is used to serve as a base having a primary net structure. The size of holes within the net is preferably about 100 microns to 5 mm, more preferably about 200 microns to 2 mm, and most preferably about 500 microns to 1.5 mm in the shortest dimension. Another aspect comprises a mixture of beeswax and edible oil, preferably vegetable oil (e.g., sesame oil, corn oil, peanut oil, cottonseed oil, rice bran oil, safflower oil, tea tree oil, pine nut oil, macadamia nut oil, camellia seed oil, rose hip oil, olive oil, soybean oil, or a combination thereof) or an oil extract of botanicals, is mixed with the non- woven fabric so as to impregnate the fabric with the oil-and-beeswax mixture. The concentrations of the oil and beeswax are controlled so as to ensure that a net-within-net micro structure is formed at ambient temperature (e.g., 10-25⁰C). Preferably, the concentration of beeswax in the oil-and-beeswax mixture is about 6-10%, more preferably about 7-9%, and most preferably about 7-8% by weight. The concentration of the oil or oil extract in the oil-and-beeswax mixture is preferably about 90-94%, more preferably about 91-93%, and most preferably about 92-93% by weight. Preferably, the oil-and-beeswax mixture contains minimum amount of water, e.g., less than 2%, less than 1%, or less than 0.5% water by weight.

[0015] Figure IA shows a representative microstructure of a piece of non-woven fabric impregnated with a sesame-oil-and-beeswax mixture under a microscope (magnitude: 200X). When the concentration of beeswax in the mixture of sesame oil and beeswax is about 7-8%, an ideal net- in-net microstructure formed. In contrast, as shown in Figure IB, when the concentration of beeswax in the mixture of sesame oil and beeswax is above 10%, a net- in-net microstructure is nearly invisible as beeswax clogs up the holes in the fabric.

[0016] Preferably, the micro-network structure formed by beeswax within the network of the non- woven fabric has holes of the size of 10-100 microns, more preferably 10-80 microns, and most preferably 10-60 microns in the shortest dimension.

[0017] The wound dressing with the net-within-net microstructure can be stored in a sealed package. Thus, a kit is provided that comprises the wound dressing, preferably in a sealed, sterile container, such as a plastic bag, or in a sealed aluminum foil package. The kit may further comprise an adhesive bandage, such as a self-adhesive bandage having an absorbent pad surrounded by a backing material containing an adhesive. Preferably, the adhesive pad is made of breathable fabric or polymeric material allowing air to flow through the pad. The adhesive bandage can packaged separately in a sterile container such as a paper bag. In the kit, the package containing the dressing and the adhesive bandage can be packaged together in sterile container, such as a paper bag. Depending on the use, multiple kits can be packaged in a box/case for storage, shipping and future use. [0018] Optionally, the dressing is cut to different sizes and individually packaged in a packet so as to provide versatile, convenient carrying and guaranty of sterility of the product within the packet.

[0019] As described in detail below, the inventive wound dressing also promotes wound healing by providing a physiologically moist environment, while metabolizing and removing excess exudates and toxic components produced by necrotic tissues on the wound, and further serves as a barrier to protect the wound from secondary bacterial infection and injury or contamination of environmental elements. Moreover, by using such an occlusive dressing, the user or patient can not only protect the wound but also easily dress or move around without too much concern about contamination of clothes and restriction of daily activity.

[0020] In a preferred embodiment, the oil in the wound dressing is an oil extract of botanicals. Examples of such botanicals include, but are not limited to, huangqin, huangbai, huanglian, earthworms and poppy capsules.

[0021] The oil extract of Huangqin (Scutellaria baicalensis Georgi) can be obtained by extracting huangqin in sesame oil at a temperature higher than 100⁰C, preferably at about 120-200⁰C, and most preferably 160-180⁰C. Preferably, the root of huangqin is used and may be obtained from the plant selected from one or more members of the group of Scutellaria viscidula Bge, Scutellaria amoena C. H. Wright, Scutellaria rehderiana Diels, Scutellaria ikonnikovii Juz, Scutellaria likiangensis Diels and Scutellaria hypericifolia Levl of Labiatae Family. Dictionary of Chinese Materia Medica, Shanghai Science and Technology Press, 1988, pages 2017 to 2021.

[0022] The oil extract of huangbai (Phellodendron amurense Rupr) can be obtained by extracting huangbai in sesame oil at a temperature higher than 100⁰C, preferably at about 120-200⁰C, and most preferably 160-180⁰C. Preferably, the bark of huangbai is used and may be obtained from the plant selected from one or more members of the group of Phellodendron chinense Schneid, Plellodendron chinense Scheid var. glabriusculum Schneid, Phellodendron chinense Schneid var. omeiense Huang, Phellodendron Schneid var. yunnanense Huang and Phellodendron chinense Schneid var. falcutum Huang. A Dictionary of Chinese Materia Medica, Shanghai Science and Technology Press, 1988, pages 2031 to 2035.

[0023] The oil extract of huanglian (Coptis chinensis Franch) can be obtained by extracting huanglian in sesame oil at a temperature higher than 100⁰C, preferably at about 120-200⁰C, and most preferably 160-180⁰C. The root of huanglian is preferably used. Huanglian may be selected one or more from the group of Coptis deltoidea C. Y. Cheng et Hsiao, Coptis omeiensis (Chen) C. Y. Cheng, and Coptis teetoides C. Y. Cheng of Ranunculaceae Family. A Dictionary of Chinese Materia Medica, Shanghai Science and Technology Press, 1988, pages 2022 to 2030.

[0024] The oil extract of earthworms (Pheretima aspergillum Perrier) can be obtained by extracting earthworms in sesame oil at a temperature higher than 100⁰C, preferably at about 120-200⁰C, and most preferably 160-180⁰C. Dried earthworms are preferably used.

[0025] The oil extract of poppy capsules (Papaver somniferum L.) can be obtained by extracting poppy capsules in sesame oil at a temperature higher than 100⁰C, preferably at about 120-200⁰C, and most preferably 160-180⁰C. Dried poppy capsules removed of seeds, juice and branches are preferably used.

[0026] The dry weight of each of the above botanicals in sesame oil is preferably 1-20%, more preferably 2-15%, and most preferably 2-10% of the total weight of the sesame oil.

[0027] The following are some specific advantageous features of the inventive wound dressing:

1. Maintaining a physiologically moist environment

[0028] Upon application of the inventive dressing to the wound, an almost transparent fiberous insulating membrane can form between the wound surface and the dressing. Such a membrane is an ester-protein complex formed by a combination of the active ingredients of impregnated in the fabric in the dressing, the esters at the wound, and plasma protein. There are three functions associated with such a membrane: i) forming and keeping a physiologically moist environment; ii) having the characteristics and function of a semi-permeable membrane, which allows formation of an osmotic gradient between an aqueous phase (wound tissue) and an oil phase (provided by the dressing) and enables all kinds of ingredients to exchange among themselves via ionic convection while metabolites of the wound tissues are discharged to surface of the membrane through osmosis; and nutritional ingredients in the dressing can also penetrate the membrane to reach the deep layers of the wound surface so as to fully exert their effects of promoting tissue cell regeneration and repair; iii) stimulating proliferation of epidermal cell and fibroblasts, and enhancing migration of the epidermal cells, meanwhile preventing the formation of scabs in the moist environment of the wound, thereby hastening migration of epithelial cells and shortening healing time.

2. Three "isolating" effects on tissues at the wound site

[0029] The moist physiological environment formed by the inventive wound dressing can effectively isolate the air from the wound surface to prevent damage and from secondary contamination of wound surface by the external elements. In addition, sesame oil in the dressing can reduce the damage caused by necrotic tissues, inflammatory exudates and other toxins on the wound. 3. The function of a "breathable and metabolizing" dressing

[0030] The inventive wound dressing is constructed by design to adopt a unique "net-within-net" three- dimensional micro-structure. Within the structural framework, the oil can change its phase with the change in temperature to forming a fluid channel, which facilitates the breathing and metabolism of the wound. When warmed up by the skin, the oil can permeate the wound surface and break up the necrotic tissues into particles by surrounding them, thereby initiating a series of biochemical reactions including hydrolysis, enzyme digestion, randicity, saponification, lipidification, and esterification, which hydrolyzed the large necrotic tissues into smaller tissues that are further digested enzymatically into small molecules. Such produced toxic, acidic substances are neutralized through randicity and saponification to produce non-toxic substances, which are then lipified and esterified reversibly to further reduce the toxicity of the acidic substances in the necrotic tissues, thereby minimizing further injury to the viable tissues at the wound site. At the same time the oil that has undergone such reactions loses its lipophilicity and, combined with the liquefied necrotic tissue, is discharged from the wound surface, while fresh oil from the dressing continues to permeate the wound surface, starting another round of such biochemical reactions.

[0031] Thus, in general, the present inventive wound dressing possesses the following advantageous features:

1) Accelerating healing of the wound tissues and regeneration of new skin and new tissues beneath the skin by harnessing the intrinsic regenerative potential of the body and by cultivating regenerative stem cells in situ and in vivo;

2) Easily controlled uniform application of the medicinal composition on the wound surface so as to achieve better efficacy;

3) Easy removal of the necrotic tissues without causing secondary injury to the viable tissues on the wound;

4) Significantly reducing or avoiding the pain associated with changing conventional wound dressings;

5) Convenient use without affecting daily life and activity;

6) Allowing convenient outpatient use and longer intervals between the changes of dressing. [0032] An aspect of the present invention also provides a method of treating a wound of the skin, bone, mucusa, tendon, muscle, nerve, or connective tissue in a mammal by applying topically the inventive wound dressing to cover the wound of the animal. The animal is preferably a human, but can be a pet or a livestock animal, such as a cat, dog, horse, mouse, rat, rabbit, tiger, elephant, pig, bull and cow.

[0033] The method may be used to treat acute wounds such as a wound resulted from physical trauma, surgery, thermal, wind, frost, chemical burns, optical or electric injury, including but not limited to burns, scalds, wounds of skin graft donor sites, ostomy, surgical incisions and sutures (including cosmetic or plastic surgery).

[0034] The method may also be used to treat chronic wounds such as chronic surface ulcers, diabetic ulcers, decubital ulcers, bedsores, chronic wounds as a result of a lower extremity vascular disease, chronic wounds as a result of poor blood flow, wounds due to skin cancer or cancer metastasis, roentgen ulcers, erosion caused by bacterial or viral infection, herpes simplex corneal ulcer, subcutaneous tissue ulcer, radiation-caused skin ulcer, vaginitis, cervical erosion, gingivitis, wounds due to dilation and enlargement of veins, and hemorrhoids.

[0035] Optionally, the method may further comprise: debriding the wound before applying the dressing to the wound, particularly for chronic wounds. The debridement may include surgically removing necrotic tissues from the wound or chemically removing necrotic tissues from the wound, while avoiding injury to the viable tissue surrounding the wound site.

[0036] According to another aspect of the present invention, the dressing can be prepared by taking the following steps: mixing a piece of reticular fabric with a mixture of edible oil or oil extract of botanicals and beeswax; heating the mixture of the fabric and the oil-beeswax mixture to a temperature of about 70-130⁰C such that the oil-beeswax mixture becomes fluidic; taking out the fabric impregnated with the fluidic oil-beeswax mixture; and cooling the fabric impregnated with the oil-beeswax mixture at a temperature below 35°C. The cooled fabric impregnated with the oil-beeswax mixture can be packaged in a sterile container, such as a sterile plastic bag for storage and future use.

[0037] In the preparation of the inventive dressing, the edible oil or oil extract of botanicals is preferably premixed with beeswax. When the mixture of edible oil or oil extract of botanicals and beeswax is heated to a temperature of about 70-180⁰C, and then cooled down to an ambient temperature below 35⁰C in the presence of a non-woven fabric, a net-in-net microstructure is formed. By exploring differential thermo-physical characteristics of beeswax, oil, reticular fabric at the different temperatures, the inventors designed and successfully enabled the inventive wound dressing having a unique net-within-net three-dimensional microstructure. The inventors appreciate that beeswax has a higher melting point than the edible oil (e.g., sesame oil); beeswax is solid at room temperature and melts when heated to a temperature above 70-80⁰C. When melted, the beeswax can be mixed with the oil with adequate stirring. However, when the mixture of beeswax and sesame oil (or sesame oil extract of botanicals) is allowed to cool down to an ambient temperature below 35⁰C, beeswax cools down much faster than the oil due to their differential thermo-physical properties. In the presence of a reticular fabric and within the holes of the fabric, the solidifying beeswax starts to form a net-like microstructure and within the holes of the beeswax network micro-droplets of the oil are enclosed, therefore forming a net-within-net three-dimensional microstructure at ambient temperature. [0038] Preferably, non-woven spunlace or needle felt fabric is used as the reticular fabric and is cut to a predetermined size before mixing with the oil-beeswax mixture.

[0039] Also preferably, the edible oil is sesame oil or sesame oil extract of botanicals. The sesame oil- beeswax mixture can be mixed with the reticular fabric in a container with wall of stainless steel or glass.

[0040] Yet preferably, the mixture of the fabric and the oil-beeswax mixture can be heated to a temperature of about 70-130⁰C for about 10-30 minutes such that the sesame oil and beeswax mixture becomes fluidic. [0041] Yet preferably, the fabric is allowed to be soaked in the oil-beeswax mixture for about 10-60 minutes, more preferably for 10-30 minutes, before being taken out.

[0042] Yet preferably, the fabric taken out of the oil-beeswax mixture is cooled at a temperature of about 4-3O⁰C, preferably 10-25⁰C, for about 10-60 minutes, more preferably for about 10-30 minutes, without contacting other objects. [0043] The present invention also provides a dressing prepared in a process comprising the steps of:

Mixing a piece of reticular fabric with a mixture of edible oil or oil extract of botanicals and beeswax;

Heating the mixture of the fabric and the oil-beeswax mixture to a temperature of about

70-130⁰C such that the oil-beeswax mixture becomes fluidic;

Taking out the fabric impregnated with the fluidic oil-beeswax mixture; and

Cooling the fabric impregnated with the oil-beeswax mixture at a temperature below

35°C. EXAMPLES

1. Preparation of a wound dressing of non-woven fabric impregnated with a sesame oil and beeswax mixture

[0044] This embodiment of the present invention is prepared by taking the following steps:

Providing a mixture of sesame oil and beeswax, wherein the concentration of beeswax in the mixture of edible oil and beeswax is about 6-10% by weight;

Mixing a piece of non- woven spunlace or needle-felt fabric with the oil-beeswax mixture in a container with wall of stainless steel or glass;

Heating the mixture of the fabric and the oil-beeswax mixture to 70-130⁰C for 10-30 minutes such that the sesame oil and beeswax mixture becomes fluidic;

Soaking the fabric in the heated mixture of sesame oil and beeswax for 10-30 minutes;

Taking out the soaked fabric from the oil-beeswax mixture, leaving it intact at 10-25°C for about 10 minutes (without contacting other objects);

Putting the fabric impregnated with the oil-beeswax mixture in a sterile aluminum foil package; and

Sealing the aluminum foil package.

2. Preparation of a wound dressing of non-woven fabric impregnated with a sesame oil extract of botanicals and beeswax mixture

[0045] This embodiment of the present invention is prepared by taking the following steps:

Providing a mixture of beeswax and sesame oil extract of a botanical selected from the group consisting of huangqin, huangbai, huanglian, earthworms and poppy capsules, wherein the concentration of beeswax in the mixture of edible oil and beeswax is about 6-

10% by weight; and wherein said botanical is comprised in about 1-30% by weight.;

Putting the fabric impregnated with the oil-beeswax mixture in a sterile aluminum foil package; and Sealing the aluminum foil package.

3. Preparation of a kit containing the wound dressing in Example 1 or 2

[0046] The wound dressing contained in a sealed sterile aluminum foil package as prepared in Example 1 or 2 above is packaged together with an adhesive bandage in a sterile paper bag.

4. Treatment of trauma patient with wound dressing prepared in Example 2

[0047] The wound dressing prepared in Example 2 above was used to treat an earthquake victim who was injured with an open wound exposing the bone (Figure 2A). The patient was treated with the wound dressing as prepared in Example 2. As shown in Figure 2B, the wound dressing was applied topically to cover the wound. As shown in Figure 2B, the wound dressing was affixed to her temple via a breathable adhesive bandage. Significantly, patient's wound healed with regeneration of new subcutaneous tissues and skin without obvious scarring.

[0048] While preferred embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention. It is intended that the following claims define the scope of the invention and that methods and structures within the scope of these claims and their equivalents be covered thereby.

Claims

What is claimed is:

1. A dressing, comprising: a reticular fabric impregnated with a mixture of edible oil and beeswax, and having a net- in-net microstructure at ambient temperature, wherein the concentration of beeswax in the mixture of edible oil and beeswax is about 6-10% by weight.

2. The dressing according to claim 1, wherein the concentration of beeswax in the mixture of edible oil and beeswax is about 6-10%.

3. The dressing according to claim 1, wherein the reticular fabric is a non- woven spunlace or needle- felt fabric.

4. The dressing according to claim 1, wherein the size of holes in the reticular fabric is about 100 microns to 5 mm in the shortest dimension.

5. The dressing according to claim 1, wherein the size of holes in the reticular fabric is about 200 microns to 2 mm in the shortest dimension.

6. The dressing according to claim 1, wherein the edible oil is edible vegetable oil selected from the group consisting of sesame oil, corn oil, peanut oil, cottonseed oil, rice bran oil, safflower oil, tea tree oil, pine nut oil, macadamia nut oil, camellia seed oil, rose hip oil, olive oil, soybean oil, and a combination thereof.

7. The dressing according to claim 1, wherein the edible oil is a vegetable oil extract of a botanical selected from the group consisting of huangqin, huangbai, huanglian, earthworm and poppy capsule, wherein said botanical is comprised in about 1-30% by weight.

8. The dressing according to claim 1, wherein the microstructure has a micro-network formed by beeswax within the network of the reticular fabric.

9. The dressing according to claim 8, wherein a micro-network formed by beeswax within the network of the reticular fabric has holes of the size of about 10-100 microns in the shortest dimension.

10. The dressing according to claim 1, wherein the holes of the micro-network formed by beeswax within the network of the reticular fabric are filled with the edible oil.

11. The dressing according to claim 1, wherein the dressing is contained in a sealed sterile container.

12. The dressing according to claim 11, wherein the container is made of plastic or aluminum foil.

13. A kit, comprising: a dressing accrodign to claim 1 and an instruction to use.

14. The kit according to claim 13, wherein the dressing is contained in a sealed sterile container in the kit.

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15. The kit according to claim 13, further comprising: an adhesive bandage for affixing the dressing to a surface.

16. The kit according to claim 15, wherein the surface is the skin of an animal.

17. The kit according to claim 16, wherein the animal is selected from a human, a pet animal, a zoo animal, and a livestock animal.

18. The kit according to claim 15, wherein the dressing and the adhesive bandage are contained in a sealed sterile package.

19. A method of preparing a dressing, comprising the steps of: mixing a piece of reticular fabric with a mixture of edible oil or oil extract of botanical and beeswax, wherein the concentration of beeswax is about 6-10%; heating the mixture of the fabric and the oil-beeswax mixture to a temperature of about

70-130⁰C such that the oil-beeswax mixture becomes fluidic; taking out the fabric impregnated with the fluidic oil-beeswax mixture; and cooling the fabric impregnated with the oil-beeswax mixture at a temperature below

35⁰C.

20. The method according to claim 19, wherein the oil extract of botanical is sesame oil extract of a botanical selected from the group consisting of huangqin, huangbai, huanglian, earthworm, poppy capsule and a combination thereof.

21. The method according to claim 19, wherein the mixing of the reticular fabric with the mixture of edible oil and beeswax is performed in a container with wall of stainless steel or glass.

22. The method according to claim 19, wherein the heating of the mixture of the fabric and the oil- beeswax mixture is heating to a temperature of about 70-130⁰C for about 10-30 minutes such that the sesame oil and beeswax mixture becomes fluidic.

23. The method according to claim 19, further comprising: soaking the reticular fabric in the mixture of edible oil and beeswax for about 10-60 minutes before being the taking out step.

24. The method according to claim 19, wherein cooling the fabric impregnated with the oil-beeswax mixture is cooling at a temperature of about 10-25⁰C for about 10-60 minutes.

25. The method according to claim 19, further comprising: packaging the cooled fabric impregnated with the oil-beeswax mixture in a sterile container; and sealing the container.

26. A dressing prepared by the process of any one of claims 19-25.

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