AU698986B2 - Cutaneous rejuvenating and healing product, method for its manufacture and uses thereof - Google Patents

Abstract

Cutaneous rejuvenating and healing product comprising ground material made up of the mother of pearl testa of a bivalved mollusc and the cell dilacerate of the mollusc mantle. After being enzymatically and thermally treated, both phases are blended to a gel or cream, before being combined with excipients such as menthol, camphor, benzoin, citric acid, methyl oxibenzoate, cetylic alcohol, stearylic alcohol, officinal vaseline, glycerine, purified water, algae extract, calcium hydroxide and copra extract. The product of the invention is for dermatological and cosmetic use.

Description

Wimp Cutaneous rejuvenating and healing product. method for its manufacture and uses thereof The present invention concerns a product manufactured from the mineral and/or organic phases emanating from Pinctada Maxima, Margaritifera or any other aquatic bivalved mollusc, gastropod of cephalopod, intended to accelerate the process of rejuvenating and healing wounds.

It is known that during substance losses through wounds, operations or degenerative diseases, burns or bed sores, the healing process appears in two different ways: a) first, healing by placing together and suturing the edges of the wound, to protect the deep layers of the wound, b) second, healing by connective granulation of the deep layers to make up the substance loss and thereafter healing the superficial layers of the wound. This latter process is necessarily much longer than the former.

In order to accelerate these processes, many types of substances are j used to protect the wound and to avoid or fight contamination. These are j most often creams or substances containing antiseptics, antibiotics, vitamin A and plants. Despite these precautions and these treatments the healing processes fail sometimes for many intrinsic or extrinsic reasons.

H-owever, whatever healing type or treatments are used, complete tissue rejuvenating requires between 45 to 60 days.

J The product according to the invention is obtained, on the one hand, directly from a ground material made up of the freeze-dried cells of the mnollusc mnantle and, on the other hand, from the ground material of the pearl 25 testa of the mollusc from which either the organic fraction or the mineral fraction is used, or both.

I *Throughout this specification, unless the context requires otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element, integer or step, or group of elements, integers or steps, but not the exclusion of any other element, integer or step, or group of elements, integers or steps.

In a first aspect, the present invention is directed to a method of preparing a cutaneous healing product comprising the steps of: a) subjecting cells from a mantle of a bivalved mnollusc selected from the group consisting of Pinctada Maxima, Margaritifera, gastropod molluscs and cephalopod molluscs to a thermal treatmnent in a vacuum and filtering on a 'A 0 -44 -V 2 diaphragm; b) concentrating the cells from the mantle by centrifugation; c) freeze-drying said thermally-treated cells; d) crushing and grinding a pearl testa of a bivalved mollusc selected from the group consisting of Pinctada Maxima, Margaritifera, gastropod molluscs and cephalopod molluscs; e) subjecting the ground pearl testa to an ultrasound treatment and then to an enzymatic treatment; f) subjecting the material from step e) to a chemical acid treatment to deactivate the enzyme; and g) mixing the material obtained in step c) with the material obtained in stepf.

In a second aspect, the present invention is directed to a cutaneous healing composition comprising an effective amount of a product obtained byA the method according to the first aspect of the invention.

The product according to the invention, in a first preferential way, is obtained in the following manner: after crushing and grinding the pearl testa of the mollusc, the obtained residue is washed and cleaned in an ultrasound jar, and is then subjected to an enzymatic liquid treatment either with carbonic anhydrase or trypsin or any other enzyme followed by an acid treatment to deactivate the enzyme.

The residue is thereafter washed with demineralized water, subjected to an evaporation treatment at 50OG over 48 hours, then thinned out with the demineralized water and centrifuged. According to a second way of 25 fabrication, a dilacerate of the mollusc mantle is submitted to a thermal treatment in a vacuum for 4 hours. The so obtained jelly is filtered on a diaphragm, concentrated by centrifugation and freeze-dried.

The product according to the invention can be obtained by mixing the C. two phases in order to obtain a gel, a cream or a solution.

The product according to the invention is mixed with vehicles having chemical properties enhancing its effects and promoting penetration. These vehicles can be as follows without being limited by this list: menthol, camphor, benzoin, citric acid, methyl oxibenzoate, cetyl alcohol, stearyl alcohol, officinal vaseline, glycerine, purified water, vitamin A, algae extract, calcium hydroxide and copra extract.

3 The product according to the invention is intended to appreciably accelerate the healing by stimulation of the cytoplasm-ic activity of the fibroblasts, inducing their proliferation in some hours, and this is due to its eutrophic and healing properties.

Some "in vivo" experiments have shown that the product according to the invention brings about an acceleration and an increase in volume of the healing bud as well as a very rapid coaptation of the wound edges.

The following examples show the applications of the product according to the invention.

In a first example, two losses of cutaneous substances having l0nun diameter concerning the epiderm and the derm are effected on an animal.

One is coated with the sterilized product according to the invention, the other is covered with an occlusive dressing. The formation of a healing bud is observed 48 hours after, with cellular proliferation and decrease of two thirds of the bleeding surface. Complete healing occurs after 6 days, whereas the control loss of substance heals after 17 days.

In a second example, the product according to the invention was sterilized and applied on a plantar perforating sore in evolution for 15 years.

It is known that the plantar perforating sore is an ailment of the skin and the mnuscles with frequent occurrence by diabetes, characterized by a perforation 9. of the planar vault at the support points, the heel and the metatarsus. This 0 loss of substance occurs as a lesion of variable diameter and depth, the edges of which are atonic because the vascularization completely disappeared, 25 which lesion is sometimes infected.. never evolving towards spontaneous 1: 25 healing and is insensitive to any treatment. In the above-mientioned example, one can see after a week a spectacular decrease of the diameter and depth of lesion as well as a net revascularization of its edges. The healing bud fills up the lesion after 15 days, the complete recovery is obtained in 3 weeks by rejuvenating of the corneal stratus.

A third example concerns the use of the product according to the ::0:invention in its cosmietologic application: after rubefaction of the right and left jugal areas, only one area is coated with the product according to the 9 invention. the other one acting as control. just after the application of the product according to the invention,. a sedation of the pains due to burns is noted, 48 hours afterwards, a partial rejuvenation of the superficial layer of the epiderm occurs with complete disparition of the hyperhem-ia and 6 days c n- i I 4 later the smooth and normal aspect of the epiderm shows evidence of a complete tissue rejuvenation of the superficial layer.

A fourth example concerns the utilization of the product according to the invention on a second degree burn: the product according to the invention is applied as a cream on the whole surface of the burnt area. As soon as the product according to the invention is applied, the sensation of burning disappears totally and a considerable diminution of the bleeding surface is clinically noted after 48 hours as well as the beginning of a new pigmentation of the epiderm.

All these experiments revealed the stimulative action of the regulated process of cellular proliferation due to the product according to the invention as well as its eutrophic, antalgic, healing and antiphlogistic properties.

One of ordinary skill in the art can prepare a gel, a cream, a solution each time he wants to obtain an eutrophic, regenerating, antalgic, healing and antiphlogistic action, having as constituent either the organomineral complex of the pearl testa of bivalved molluscs such as Pinctada Maxima and others, either a freeze-dried product of the cells of the mantle originating from the organic or mineral fraction in association with one or several above noted vehicles or substances.

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Claims (9)

1. A method of preparing a cutaneous healing product comprising the steps of: a) subjecting cells from a mantle of a bivalved mollusc selected from the group consisting of Pinctada Maxima, Margaritifera, gastropod molluscs and cephalopod molluscs to a thermal treatment in a vacuum and filtering on a diaphragm; b) concentrating the cells from the mantle by centrifugation; c) freeze-drying said thermally-treated cells; 10 d) crushing and grinding a pearl testa of a bivalved mollusc selected from the group consisting of Pinctada MaximnaMargaritifera, gastropod molluscs and cephalopod molluscs; e) subjecting the ground pearl testa to an ultrasound treatment and then to an enzymatic treatment; subjecting the material from step e) to a chemical acid treatment to deactivate the enzyme; and g) mixing the material obtained in step c) with the material obtained in step fl.

2. The method of claim 1 further comprising subjecting the material obtained in step e) to an evaporation treatment at 50 0 C prior to step fl.

3. The method of claim 1 further comprising the step of formulating the composition in the form of a gel, cream or solution.

4. The method of claim 1 wherein carbonic anhydrase or trypsin is used in the enzymatic treatment of step e) 25

5. A cutaneous healing composition comprising an effective amount of a product obtained by the method according to any one of claims I. to 4.

6. The composition of claim 5 wherein the product is in the form of a gel, cream or solution.

7. The composition of claim 5 wherein the product is blended with a vehicle selected from the group consisting of menthol, camphor, benzoin, citric acid, mnethyl oxibenzoate, stearyl alcohol, officinal vaseline, glycerine, purified water, vitamin A, algae extract, calcium hydroxide and copra extract. I 6

8. The composition of claim 5 wherein the product is blended with a vehicle that accentuates the healing effects of the product and promotes the penetration of the product into the skin. Dated this twenty-seventh day of August 1998 FORTUNE BASE MVANAGEMIENT LTD Patent Attorneys for the Applicant: F B RICE CO o 0, 00 6 0*00 00 0 .440. ABSTRACT OF THE DISCLOSURE Cutaneous rejuvenating and healing product comprising ground material made up of the mother of pearl testa of a bivalved mollusc and the cell dilacerate of the mollusc mantle. After being enzymatically and thermally treated, both phases are blended to a gel or cream, before being combined with excipients such as menthol, camphor, benzoin, citric acid, methyl oxibenzoate, cetylic alcohol, stearylic alcohol, officinal vaseline, glycerine, purified water, algae extract, calcium hydroxide and copra extract. The product of the invention is for dermatological and cosmetic use. i i i ii ii ur,. r:-e !r r -I I~- LIII~ ah.- :I I INTERNATIONAL SEARCH REPORT Intern \1 Application No PCT/FR 95/00606 A. CLASSIFICATION OF SUBJECT MATTER IPC 6 A61K35/56 A61K7/48 e a According to International Patent Clasification (IPC) or to both national classification and IPC B. FIELDS SEARCHED Minimum documentation searched (classification system followed by classification symbols) IPC 6 A61K Documentation searched other than minimum documentation to the extent that such documents are included in the fields searched Electronic data base consulted during the international search (name of data base and, where practical, search terms used) C. DOCUMENTS CONSIDERED TO BE RELEVANT Category Citation of document, with indication, where appropriate, of the relevant passages Relevant to claim No. A PATENT ABSTRACTS OF JAPAN 1-10 vol. 12, no. 279 (C-517) (3126) 1 August 1988 JP,A,63 057 507 (MIKIMOTO SEIYAKU K.K.) 12 March 1988 see abstract A FR,A,1 332 198 SCEOPUL) 16 December 1-10 1963 see the whole document A US,A,4 393 045 HENDERSON ET AL.) 12 1-10 July 1983 see the whole document A DE,A,34 37 184 IWAN ET AL.) 17 April 1-10 1986 see the whole document Further documents are listed in the continuation of box C. M Patent family members are listed in annex. SSpecial categories of cited documents: later document published after the international filing date Sdocument definin the eneral state of the art which is nt or priority date and not in conflict with the application but document de t he general state of the art which is not to ndetand the principle or theory underying the considered to be of particular relevance invention earlier document but published on or after the international X' document of particular relevance; the claimed invention filing date cannot be considered novel or cannot be considered to document which may throw doubts on priority claim(s) or involve an inventive step when the document is taken alone which is cited to establish the publicaion date of another Y document of partcular relevance; the claimed invention citation or other special reason (as specified) cannot be considered to involve an inventive step when the document referring to an oral disclosure, use, exhibition or document is combined with one or more other such docu- other means nments, such combination being obvious to a person skilled document published prior to the international filing date but in the art. later than the priority date claimed document member of the same patent family Date of the actual completion of the international search Date of mailing of the international search report 23 August 1995 29.08.95. Name and mailing address of the ISA Authorized officer European Patent Office, P.B. 5818 Patentlaan 2 NL 2280 HV Rijswijk Tel. 31-70) 340-2040, Tx. 31 651 epo il, Ryckebosch, A Fax: (+31-70) 340-3016 y O h, A Form PCTIISA/210 (second shMt) (July 1992) q V INTERNATIONAL SEARCH REPORTr Intern, -d Application No wujormatuyn on patent family mebr PCT/FR 95/00606 Patent document Publication Patent family Publication cited in search report daemember(s) Tdute FR-A-1332198 16-12-63 NONE US-A-4393045 12-07-83 NONE DE-A-3437 184 17-04-86 NONE 1Fofff PCT/ISA/210 (pwtmZt family an, nex) (July 1992) RAPPORT DE RECHERCHE INTERNATIONALE Dcmaw iteationale No PCT/FR 95/00606 A. CLASSEMENT DE L'OBJET DE LA DEMANDE CIB 6 A61K35/56 A61K7/48 Scion In classification intematioriale des brevets (CIB) ou A la fois scion In classification nationaic et la CIB B. DOMAINES SUR S;QES ARCHERCHTE A PORTE Documentation minimale consultte (systme de classification sivi des symboles de classement) CIB 6 A61K Docunentation consulte autie clue In documentation sninirnale dans la mesure ati ces documents rettvent des dosnamacs stir lesqtiels a portA la recherchse Base dle dornes electronique consultbe au cours de Ia recherche iernationale (nomn de la base de dlonntes, et si cela eat rtalisable, termes dle recherche A PATENT ABSTRACTS OF JAPAN 1-10 vol. 12, no. 279 (C-517) (3126) 1 Ao~t 1988 JP,A,63 057 507 (MIKIMOTO SEIYAKU K.K.) 12 Mars 1988 voir abr~g6 A FR,A,1 332 198 UT.N. SCEOPUL) 16 D6cembre 1-10 1963 voir le document en entier A US,A,4 393 045 HENDERSON ET AL.) 12 1-10 Juillet 1983 voir le document en entier A DE,A,34 37 184 IWAN ET AL.) 17 Avril 1-10 1986 voir le document en entier []Voir la suite du cadre C pour In fin de la liste des documents !J Les documents de families de brevets sont indiques en anex *Cattgories speciales de documents cis T' document ulttrietr publit aprts In date de dtt international oa I& docuentdtfiissnt 'tta geeralde s tchniuenondate de prioritt et nappattenenat pas A Vttat de In 'A dcuen ceinssnt1' d n ecniue nntecniupetet.aa citt pour coenprendre Ic Prinicipe cosasidert comme particultemn pertinent 01= 11 1 1 theon co iuatI as einvention document anittneur, roais publit A Ia date de dep~t international W~dcmn atclteetpriet lvninrvniuescpu oti spree cste date Cic cnsideeecom nouvell outcomme lenpfiquant ne activitt L document pouvant icier um doute stir tine revendication dle inventive par rapport au document considert isoltmerat priorit6 oti citt pour determiner la date de publication d'sme document particulitrement pertinent; l'invention rcvendiqute autre citation ou pour tine raison speciale (telle qlu'indiqlte) ne peut Eie considerte commre impliquant one activit6 inventive document se reftrant A tine divulgation orale, A tin usage A lorsque le document eat associd A on ou plusieurs auixes one exposition oti tous autre moyens documents de matme nature, cette cormbinaison 6tant 6vidente documrent publi6 avant la date de dtpflt international, mai pour tine persone due mttier postricurement A In date de priorite revendique &W document qui fait partie de I a meme famille de brevets Date A Inquelle Ia recherche interationale a 0t6 effectivemencrt achevte Date d'exptdition du prtsenit rapport de recherche intemaionale 23 Ao~t 1995 29.08.95. Nomn et adresse postale de I'administration chargte de Ia recherche internationale Fonictionnaire autost Office Europeen des Breves, P.B. 58118 Patentlaan 2 NL 2280 HV Rijswijk Tel. 31-70) 340-M040, Tx.-31 651 epo nl,Rykb ch A Fax: 31.70) 340-3016 y kb sh A Famiulalse PCT/ISA/210 (deuaiins teufli) (jufltat 1992) 4, I-. RAPPORT DE RECHERCHE INTERNATIONALE Dcm itnh, temonhic No Renseignements relatifs aux ruvimbres de famfila de bmetsPC FR

9/ Document brevet cit6 Date de I Membre(s) de laDaed au rapport de recherche publication fanile de brevet(s) I pubficaion FR-A-1332198 16-12-63 AUCUN US-A-4393045 12-07-83 AUCUN DE-A-3437 184 17-04-86 AUCUN i Formulalin PCT/ISA]210 (IMnl ftmjuu do brevals) IjuilsltU192