CN100411638C - Steam distillate from soybean and its composition - Google Patents
Steam distillate from soybean and its composition Download PDFInfo
- Publication number
- CN100411638C CN100411638C CNB2003101005964A CN200310100596A CN100411638C CN 100411638 C CN100411638 C CN 100411638C CN B2003101005964 A CNB2003101005964 A CN B2003101005964A CN 200310100596 A CN200310100596 A CN 200310100596A CN 100411638 C CN100411638 C CN 100411638C
- Authority
- CN
- China
- Prior art keywords
- steam distillation
- distillation thing
- treatment
- minutes
- injury
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 71
- 244000068988 Glycine max Species 0.000 title claims abstract description 37
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 37
- 230000003779 hair growth Effects 0.000 claims abstract description 35
- 239000000287 crude extract Substances 0.000 claims abstract description 34
- 208000028990 Skin injury Diseases 0.000 claims abstract description 32
- 206010048768 Dermatosis Diseases 0.000 claims abstract description 30
- 208000017520 skin disease Diseases 0.000 claims abstract description 30
- 230000008929 regeneration Effects 0.000 claims abstract description 19
- 238000011069 regeneration method Methods 0.000 claims abstract description 19
- 238000001704 evaporation Methods 0.000 claims abstract description 14
- 208000027418 Wounds and injury Diseases 0.000 claims description 108
- 238000011282 treatment Methods 0.000 claims description 105
- 206010052428 Wound Diseases 0.000 claims description 95
- 238000001256 steam distillation Methods 0.000 claims description 85
- 210000000582 semen Anatomy 0.000 claims description 67
- 238000000034 method Methods 0.000 claims description 35
- 239000002994 raw material Substances 0.000 claims description 32
- 230000000694 effects Effects 0.000 claims description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 26
- GLDOVTGHNKAZLK-UHFFFAOYSA-N octadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCCCO GLDOVTGHNKAZLK-UHFFFAOYSA-N 0.000 claims description 20
- 230000004936 stimulating effect Effects 0.000 claims description 19
- 208000025865 Ulcer Diseases 0.000 claims description 18
- 210000003780 hair follicle Anatomy 0.000 claims description 18
- 230000007794 irritation Effects 0.000 claims description 18
- 231100000397 ulcer Toxicity 0.000 claims description 18
- 239000003814 drug Substances 0.000 claims description 17
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 17
- 230000006378 damage Effects 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 15
- 201000004624 Dermatitis Diseases 0.000 claims description 14
- 206010012601 diabetes mellitus Diseases 0.000 claims description 14
- 208000014674 injury Diseases 0.000 claims description 14
- 206010012438 Dermatitis atopic Diseases 0.000 claims description 13
- 201000008937 atopic dermatitis Diseases 0.000 claims description 13
- 230000008020 evaporation Effects 0.000 claims description 13
- 239000003643 water by type Substances 0.000 claims description 13
- 230000001684 chronic effect Effects 0.000 claims description 12
- DTGKSKDOIYIVQL-WEDXCCLWSA-N (+)-borneol Chemical compound C1C[C@@]2(C)[C@@H](O)C[C@@H]1C2(C)C DTGKSKDOIYIVQL-WEDXCCLWSA-N 0.000 claims description 11
- 206010014080 Ecchymosis Diseases 0.000 claims description 11
- 239000001818 polyoxyethylene sorbitan monostearate Substances 0.000 claims description 11
- 235000010989 polyoxyethylene sorbitan monostearate Nutrition 0.000 claims description 11
- 235000021355 Stearic acid Nutrition 0.000 claims description 10
- 239000007766 cera flava Substances 0.000 claims description 10
- BXWNKGSJHAJOGX-UHFFFAOYSA-N hexadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCO BXWNKGSJHAJOGX-UHFFFAOYSA-N 0.000 claims description 10
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 claims description 10
- GOQYKNQRPGWPLP-UHFFFAOYSA-N n-heptadecyl alcohol Natural products CCCCCCCCCCCCCCCCCO GOQYKNQRPGWPLP-UHFFFAOYSA-N 0.000 claims description 10
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 claims description 10
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 claims description 10
- 239000008117 stearic acid Substances 0.000 claims description 10
- 240000004922 Vigna radiata Species 0.000 claims description 9
- 235000010721 Vigna radiata var radiata Nutrition 0.000 claims description 9
- 230000007547 defect Effects 0.000 claims description 9
- 230000001476 alcoholic effect Effects 0.000 claims description 8
- 230000009693 chronic damage Effects 0.000 claims description 8
- 230000003115 biocidal effect Effects 0.000 claims description 7
- 235000008216 herbs Nutrition 0.000 claims description 7
- 230000009692 acute damage Effects 0.000 claims description 6
- 230000001154 acute effect Effects 0.000 claims description 6
- 206010017711 Gangrene Diseases 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 3
- 230000037380 skin damage Effects 0.000 claims 8
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 claims 4
- 230000000844 anti-bacterial effect Effects 0.000 claims 3
- 230000000638 stimulation Effects 0.000 abstract 1
- 241000700159 Rattus Species 0.000 description 48
- 239000002674 ointment Substances 0.000 description 48
- 210000003491 skin Anatomy 0.000 description 34
- 239000000284 extract Substances 0.000 description 19
- 238000011156 evaluation Methods 0.000 description 17
- 230000003203 everyday effect Effects 0.000 description 16
- 229960003600 silver sulfadiazine Drugs 0.000 description 13
- UEJSSZHHYBHCEL-UHFFFAOYSA-N silver(1+) sulfadiazinate Chemical compound [Ag+].C1=CC(N)=CC=C1S(=O)(=O)[N-]C1=NC=CC=N1 UEJSSZHHYBHCEL-UHFFFAOYSA-N 0.000 description 13
- 238000002651 drug therapy Methods 0.000 description 10
- 238000010562 histological examination Methods 0.000 description 10
- 230000029663 wound healing Effects 0.000 description 10
- 239000002537 cosmetic Substances 0.000 description 8
- 230000005855 radiation Effects 0.000 description 8
- 206010028980 Neoplasm Diseases 0.000 description 7
- 244000000231 Sesamum indicum Species 0.000 description 7
- 235000003434 Sesamum indicum Nutrition 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 7
- 210000004209 hair Anatomy 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 6
- 241000283973 Oryctolagus cuniculus Species 0.000 description 6
- 240000007711 Peperomia pellucida Species 0.000 description 6
- 206010000496 acne Diseases 0.000 description 6
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 230000037311 normal skin Effects 0.000 description 6
- 240000001745 Rheum palmatum Species 0.000 description 5
- 235000008090 Rheum palmatum Nutrition 0.000 description 5
- 210000000589 cicatrix Anatomy 0.000 description 5
- 230000035617 depilation Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- WVTKBKWTSCPRNU-KYJUHHDHSA-N (+)-Tetrandrine Chemical compound C([C@H]1C=2C=C(C(=CC=2CCN1C)OC)O1)C(C=C2)=CC=C2OC(=C2)C(OC)=CC=C2C[C@@H]2N(C)CCC3=CC(OC)=C(OC)C1=C23 WVTKBKWTSCPRNU-KYJUHHDHSA-N 0.000 description 4
- 206010002198 Anaphylactic reaction Diseases 0.000 description 4
- 206010015150 Erythema Diseases 0.000 description 4
- 206010020565 Hyperaemia Diseases 0.000 description 4
- 206010061218 Inflammation Diseases 0.000 description 4
- 241000972673 Phellodendron amurense Species 0.000 description 4
- -1 alkyl peroxide free radical Chemical class 0.000 description 4
- 208000003455 anaphylaxis Diseases 0.000 description 4
- 201000011510 cancer Diseases 0.000 description 4
- 231100000321 erythema Toxicity 0.000 description 4
- 239000000835 fiber Substances 0.000 description 4
- 210000002683 foot Anatomy 0.000 description 4
- 230000035876 healing Effects 0.000 description 4
- 230000004054 inflammatory process Effects 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 235000019198 oils Nutrition 0.000 description 4
- 150000003254 radicals Chemical class 0.000 description 4
- 231100000241 scar Toxicity 0.000 description 4
- 238000012453 sprague-dawley rat model Methods 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- 208000002874 Acne Vulgaris Diseases 0.000 description 3
- 206010002091 Anaesthesia Diseases 0.000 description 3
- 208000019300 CLIPPERS Diseases 0.000 description 3
- 102000008186 Collagen Human genes 0.000 description 3
- 108010035532 Collagen Proteins 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 206010051814 Eschar Diseases 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 238000008214 LDL Cholesterol Methods 0.000 description 3
- 206010039203 Road traffic accident Diseases 0.000 description 3
- 206010039509 Scab Diseases 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 206010042674 Swelling Diseases 0.000 description 3
- 230000037005 anaesthesia Effects 0.000 description 3
- 230000036783 anaphylactic response Effects 0.000 description 3
- 244000309464 bull Species 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 208000021930 chronic lymphocytic inflammation with pontine perivascular enhancement responsive to steroids Diseases 0.000 description 3
- 229920001436 collagen Polymers 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 210000004207 dermis Anatomy 0.000 description 3
- 230000002500 effect on skin Effects 0.000 description 3
- 210000002615 epidermis Anatomy 0.000 description 3
- 231100000333 eschar Toxicity 0.000 description 3
- 239000000262 estrogen Substances 0.000 description 3
- 238000005194 fractionation Methods 0.000 description 3
- 229930182470 glycoside Natural products 0.000 description 3
- 150000002338 glycosides Chemical class 0.000 description 3
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 3
- 235000008696 isoflavones Nutrition 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 210000000518 sarcolemma Anatomy 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 235000020712 soy bean extract Nutrition 0.000 description 3
- 230000008961 swelling Effects 0.000 description 3
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 2
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 2
- 206010012434 Dermatitis allergic Diseases 0.000 description 2
- 102000016942 Elastin Human genes 0.000 description 2
- 108010014258 Elastin Proteins 0.000 description 2
- YQEZLKZALYSWHR-UHFFFAOYSA-N Ketamine Chemical compound C=1C=CC=C(Cl)C=1C1(NC)CCCCC1=O YQEZLKZALYSWHR-UHFFFAOYSA-N 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- CWEZAWNPTYBADX-UHFFFAOYSA-N Procyanidin Natural products OC1C(OC2C(O)C(Oc3c2c(O)cc(O)c3C4C(O)C(Oc5cc(O)cc(O)c45)c6ccc(O)c(O)c6)c7ccc(O)c(O)c7)c8c(O)cc(O)cc8OC1c9ccc(O)c(O)c9 CWEZAWNPTYBADX-UHFFFAOYSA-N 0.000 description 2
- 208000003251 Pruritus Diseases 0.000 description 2
- 206010037888 Rash pustular Diseases 0.000 description 2
- 206010040914 Skin reaction Diseases 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- 230000002421 anti-septic effect Effects 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 210000003719 b-lymphocyte Anatomy 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- ZQSIJRDFPHDXIC-UHFFFAOYSA-N daidzein Chemical compound C1=CC(O)=CC=C1C1=COC2=CC(O)=CC=C2C1=O ZQSIJRDFPHDXIC-UHFFFAOYSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229920002549 elastin Polymers 0.000 description 2
- 210000002919 epithelial cell Anatomy 0.000 description 2
- 210000000981 epithelium Anatomy 0.000 description 2
- 229940011871 estrogen Drugs 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000003700 hair damage Effects 0.000 description 2
- 230000003661 hair follicle regeneration Effects 0.000 description 2
- 229940039412 ketalar Drugs 0.000 description 2
- 235000010445 lecithin Nutrition 0.000 description 2
- 239000000787 lecithin Substances 0.000 description 2
- 229940067606 lecithin Drugs 0.000 description 2
- 210000000088 lip Anatomy 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 210000001699 lower leg Anatomy 0.000 description 2
- 235000013310 margarine Nutrition 0.000 description 2
- 239000003264 margarine Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 239000000419 plant extract Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 229920002414 procyanidin Polymers 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 208000029561 pustule Diseases 0.000 description 2
- WVTKBKWTSCPRNU-UHFFFAOYSA-N rac-Tetrandrin Natural products O1C(C(=CC=2CCN3C)OC)=CC=2C3CC(C=C2)=CC=C2OC(=C2)C(OC)=CC=C2CC2N(C)CCC3=CC(OC)=C(OC)C1=C23 WVTKBKWTSCPRNU-UHFFFAOYSA-N 0.000 description 2
- 208000006934 radiodermatitis Diseases 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000035483 skin reaction Effects 0.000 description 2
- 231100000430 skin reaction Toxicity 0.000 description 2
- 239000000344 soap Substances 0.000 description 2
- 235000012424 soybean oil Nutrition 0.000 description 2
- 239000003549 soybean oil Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 210000003371 toe Anatomy 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- XFZJEEAOWLFHDH-UHFFFAOYSA-N (2R,2'R,3R,3'R,4R)-3,3',4',5,7-Pentahydroxyflavan(48)-3,3',4',5,7-pentahydroxyflavan Natural products C=12OC(C=3C=C(O)C(O)=CC=3)C(O)CC2=C(O)C=C(O)C=1C(C1=C(O)C=C(O)C=C1O1)C(O)C1C1=CC=C(O)C(O)=C1 XFZJEEAOWLFHDH-UHFFFAOYSA-N 0.000 description 1
- ADVGKWPZRIDURE-UHFFFAOYSA-N 2'-Hydroxyacetanilide Chemical compound CC(=O)NC1=CC=CC=C1O ADVGKWPZRIDURE-UHFFFAOYSA-N 0.000 description 1
- LRYZPFWEZHSTHD-HEFFAWAOSA-O 2-[[(e,2s,3r)-2-formamido-3-hydroxyoctadec-4-enoxy]-hydroxyphosphoryl]oxyethyl-trimethylazanium Chemical class CCCCCCCCCCCCC\C=C\[C@@H](O)[C@@H](NC=O)COP(O)(=O)OCC[N+](C)(C)C LRYZPFWEZHSTHD-HEFFAWAOSA-O 0.000 description 1
- TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 244000077995 Coix lacryma jobi Species 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 206010011732 Cyst Diseases 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 208000010201 Exanthema Diseases 0.000 description 1
- 206010063560 Excessive granulation tissue Diseases 0.000 description 1
- 208000035874 Excoriation Diseases 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
- 208000035150 Hypercholesterolemia Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 108010028554 LDL Cholesterol Proteins 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 206010062575 Muscle contracture Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- MOJZMWJRUKIQGL-FWCKPOPSSA-N Procyanidin C2 Natural products O[C@@H]1[C@@H](c2cc(O)c(O)cc2)Oc2c([C@H]3[C@H](O)[C@@H](c4cc(O)c(O)cc4)Oc4c3c(O)cc(O)c4)c(O)cc(O)c2[C@@H]1c1c(O)cc(O)c2c1O[C@@H]([C@H](O)C2)c1cc(O)c(O)cc1 MOJZMWJRUKIQGL-FWCKPOPSSA-N 0.000 description 1
- 206010063562 Radiation skin injury Diseases 0.000 description 1
- 230000002292 Radical scavenging effect Effects 0.000 description 1
- 241000219061 Rheum Species 0.000 description 1
- 206010072170 Skin wound Diseases 0.000 description 1
- 108010073771 Soybean Proteins Proteins 0.000 description 1
- 206010042496 Sunburn Diseases 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 241000282894 Sus scrofa domesticus Species 0.000 description 1
- 240000002299 Symphytum officinale Species 0.000 description 1
- 235000005865 Symphytum officinale Nutrition 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- DWCSNWXARWMZTG-UHFFFAOYSA-N Trigonegenin A Natural products CC1C(C2(CCC3C4(C)CCC(O)C=C4CCC3C2C2)C)C2OC11CCC(C)CO1 DWCSNWXARWMZTG-UHFFFAOYSA-N 0.000 description 1
- 208000024780 Urticaria Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 206010048218 Xeroderma Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000002156 adsorbate Substances 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 150000001338 aliphatic hydrocarbons Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003276 anti-hypertensive effect Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000000680 avirulence Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 201000008275 breast carcinoma Diseases 0.000 description 1
- 235000014121 butter Nutrition 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 208000037976 chronic inflammation Diseases 0.000 description 1
- 208000037893 chronic inflammatory disorder Diseases 0.000 description 1
- 239000012459 cleaning agent Substances 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 208000006111 contracture Diseases 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 208000031513 cyst Diseases 0.000 description 1
- 235000007240 daidzein Nutrition 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- RXKJFZQQPQGTFL-UHFFFAOYSA-N dihydroxyacetone Chemical compound OCC(=O)CO RXKJFZQQPQGTFL-UHFFFAOYSA-N 0.000 description 1
- WQLVFSAGQJTQCK-VKROHFNGSA-N diosgenin Chemical compound O([C@@H]1[C@@H]([C@]2(CC[C@@H]3[C@@]4(C)CC[C@H](O)CC4=CC[C@H]3[C@@H]2C1)C)[C@@H]1C)[C@]11CC[C@@H](C)CO1 WQLVFSAGQJTQCK-VKROHFNGSA-N 0.000 description 1
- WQLVFSAGQJTQCK-UHFFFAOYSA-N diosgenin Natural products CC1C(C2(CCC3C4(C)CCC(O)CC4=CCC3C2C2)C)C2OC11CCC(C)CO1 WQLVFSAGQJTQCK-UHFFFAOYSA-N 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000010894 electron beam technology Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003979 eosinophil Anatomy 0.000 description 1
- 210000005081 epithelial layer Anatomy 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 201000005884 exanthem Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- TZBJGXHYKVUXJN-UHFFFAOYSA-N genistein Natural products C1=CC(O)=CC=C1C1=COC2=CC(O)=CC(O)=C2C1=O TZBJGXHYKVUXJN-UHFFFAOYSA-N 0.000 description 1
- 229940045109 genistein Drugs 0.000 description 1
- 235000006539 genistein Nutrition 0.000 description 1
- ZCOLJUOHXJRHDI-CMWLGVBASA-N genistein 7-O-beta-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC(O)=C2C(=O)C(C=3C=CC(O)=CC=3)=COC2=C1 ZCOLJUOHXJRHDI-CMWLGVBASA-N 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 229940005582 gotu kola extract Drugs 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 210000001126 granulation tissue Anatomy 0.000 description 1
- 235000009569 green tea Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 230000002443 hepatoprotective effect Effects 0.000 description 1
- PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 description 1
- 208000000069 hyperpigmentation Diseases 0.000 description 1
- 230000003810 hyperpigmentation Effects 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 206010021198 ichthyosis Diseases 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000000976 ink Substances 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000003212 lipotrophic effect Effects 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 210000004303 peritoneum Anatomy 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 229940068065 phytosterols Drugs 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- HGVVOUNEGQIPMS-UHFFFAOYSA-N procyanidin Chemical compound O1C2=CC(O)=CC(O)=C2C(O)C(O)C1(C=1C=C(O)C(O)=CC=1)OC1CC2=C(O)C=C(O)C=C2OC1C1=CC=C(O)C(O)=C1 HGVVOUNEGQIPMS-UHFFFAOYSA-N 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 201000001514 prostate carcinoma Diseases 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 206010037844 rash Diseases 0.000 description 1
- 230000001739 rebound effect Effects 0.000 description 1
- 201000005404 rubella Diseases 0.000 description 1
- 235000012045 salad Nutrition 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 235000011121 sodium hydroxide Nutrition 0.000 description 1
- 235000019710 soybean protein Nutrition 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 229930193551 sterin Natural products 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000012209 synthetic fiber Substances 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 229940125379 topical corticosteroid Drugs 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 239000000717 tumor promoter Substances 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Images
Landscapes
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention mainly relates to a steam distillate obtained from soybean (Glycinemax(L.) Merr) seeds and a medicinal composition thereof, and the steam distillate is prepared by evaporating a crude extract. The present invention also provides the applications of the steam distillate to the cure of skin injuries and dermatosis and the stimulation to cell regeneration and hair growth.
Description
Technical field
The present invention relates generally to the steam distillation thing that obtains from Semen sojae atricolor (Glycine max (L.) Merr.) seed, and it has the effect of treatment skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth.
Background technology
Semen sojae atricolor (Glycine max (L.) Merr.) comprises Semen sojae atricolor and Semen sojae atricolor, and it is one of most important oil and protein source in the world.For example, Semen sojae atricolor can be processed into and be contained the semiarid oil of an edible, and it can be used as salad oil or is used to make margarine and butter.Soybean oil also can be used for making coating, malthoid, oilcloth, printing ink, soap, insecticide and disinfectant.In addition, the lecithin phosphide that obtains in the side-product of oil plant industry can be used as wetting agent and the stabilizing agent in food, cosmetics, medicine, leather, coating, plastics, soap and the cleaning agent.Semen sojae atricolor powder is that the domestic animal of a rich in proteins is used feedstuff.In addition, soybean protein can be used for making synthetic fibers, binding agent, textile sizing, waterproof, fire foam etc.
On medical science was used, oneself had effect to numerous disease by the report Semen sojae atricolor.
Semen sojae atricolor can be used as one and regulates the trophism additive of the function of intestinal, heart, kidney, liver and stomach.Because soybean oil contains a large amount of unsaturated fatty acids, so it can be used for anti-hypercholesterolemiccompounds.The medical lecithin that obtains from Semen sojae atricolor can be used as a lipotropic.In addition, people oneself know as the σ-steroid of an anti-hardening factor and as the sitoesterol of the substitute of diosgenin in some antihypertensive and can prepare from Semen sojae atricolor.The isoflavone of finding in Semen sojae atricolor and plant-estrogen have shown to have preventive effect to comprising breast carcinoma, carcinoma of prostate and colon cancer in interior various cancers, referring to " plant-estrogen and cancer " (Adlercreutz, H.; Phyto-oestrogens and cancer.The Lancet Oncology, 2002, Vol.3, p.364-373).People also find, edible margarine through adding phytosterol can reduce the total plasma cholesterol in person in middle and old age's hypercholesterolemia individuality and the concentration of LDL-cholesterol, referring to " the thin beef of strand that every day, edible potion added the bean plant sterin can reduce the serum total cholesterol and the LDL cholesterol of the young and middle-aged hypercholesterolemiapatients patients of male " (Matvienko, O.A., Lewis, D.S., Swanson, M., Aendt, B., Rainwater, D.L., Stewart, J., and Alekel, D.L.; A Single dailydose of soybean phytosterols in ground beef decreases serumtotal cholesterol and LDL cholesterol in young, mildlyhypercholesterolemic men.Am J Clin Nutr., 2002,76, p.57-64).
Some soybean extract also has the medicine effect through report.Take from 1 of 70% aqueous acetone extract that brown Semen sojae atricolor mutation (Akita-Zairai) plants skin, 1-diphenyl-2-trinitrophenyl-hydrazine (DPPH) free radical is removed activity and is disclosed in " have high free radical in the brown episperm of Semen sojae atricolor and remove active high polymerization procyanidin " (Takahata.Y., O.-Kameyama, M., Furuta, S., Takahashi, M., and Suda, I.; Highly polymerized procyanidins in brown soybean seed coatwith a high radical-scavenging activity.J.Agric.Food Chem., 2001,49, p.5843-5847).People find, the extract of taking from embryo extract, Semen sojae atricolor, Testa oryzae, tear grass, Semen Sesami, Semen Tritici aestivi, Fructus Citri, green tea, greenery extract and germination Oryza glutinosa is being lower than under 60 ℃ through slowly baking and use the aspergillus oryzae fermentation can have antioxidation more than 3 days after each component is converted into low molecular weight substance of an electric oven, referring to " through the antioxidation of machined valley based food " (Minamiyama, Y., Yoshikawa, T., Tanigawa, T., Takahashi, S., Naito, Y., Ichikawa, H. reach Kondo, M.; Antioxidative effects of a processed grain food.J.Nutr.Sci.Vitaminol., 1994,40, p.467-477).Radix Dauci Sativae, crucifer and comprise Semen sojae atricolor, Semen Phaseoli, Semen phaseoli radiati and Semen sojae atricolor and show that at the extract that boils of interior beans greenery having very strong anticancer promoter and free radical removes active, referring to " high correlation between lipoperoxide free radical and tumor-promoter effect: various plant extracts are to the inhibition of tumor proliferative in Epstein-Barr virus/B-lymphocyte system and to the removing of alkyl peroxide free radical " (Maeda.H., Katsuki, T., Akaike, T. and Yasutake.R.; High correlation between lipid peroxide radical andtumor-promoter effect:suppression of tumor promotion in theEpstein-Barr virus/B-lymphocyte system and scavenging ofalkyl peroxide radicals by various vegetable extracts.Jpn.J.Cancer Res., 1992,83, p.923-928).Activity by serum glutaminic acid-oxalic acid-transaminase (sGOT) and serum glutaminic acid-acetone acid-transaminase (sGPT) in the measurement rat, the water extract of Semen sojae atricolor also shows that the hepatopathy to being caused by acetyl aminophenol has effect, referring to " to the evaluation of fabaceous liver protective effect " (Wu, S.-J., Wang, J.-S. and Chang C.-H.; Evaluation of hepatoprotective activity oflegumes.Phytomedicine, 2001, Vol.8 (3), p.213-219).The soybean seed extract that uses an extract of being made up of the hybrid system of low aliphatic alcohol and low boiling ester to obtain is shown as a beverage preparation (No. the 1117767th, Japan Patent).
It is found that some specific soybean extract can be used for treating in some dermopathic cosmetics or the medicine.
The soybean extract that contains the sphingomyelins of requirement ratio and phospholipid can be used for treating in the cosmetics of dry skin (U.S. Patent Publication case US2002/0009509A1 number) through announcement.These extracts can be by independent use aliphatic alcohol or itself and water the sophisticated whole soybeans of mixture extraction or do not have oily Semen sojae atricolor powder and produce, and use aliphatic hydrocarbon and aliphatic ketone to handle subsequently.So this extract is fat-soluble.
It is found that, one cosmetic composition, medicine for treating comedo or the blackhead formation inhibitor that contains one or more plant extract (it is selected from milk surum, an and Cortex Phellodendri extract, and further is selected from one or more extract of Radix Scutellariae, comfrey and Semen sojae atricolor (Glycine max (L.) Merrill)) is to prevention or treatment dermatosis (for example acne or by the inflammation that acne the cause skin of chapping) effectively (No. the 2001097842nd, Japan Patent).Said composition can be used for making common beverages, food and person like that.
The malonyl isoflavone candy glycosides that obtains in the water extract of a Semen sojae atricolor or a Semen sojae atricolor, for example, malonyl isoflavone aglycone etc. can be used as an epithelial hyperplasia promoter (No. the 9059166th, Japan Patent).This candy glycosides obtains by following step: under 45-65 ℃ peeled soybeans immersed with caustic soda and be adjusted in the water of pH7.5 to pH9.0 2-4 hour, remove Semen sojae atricolor to obtain steep water as water extract, use a ultrafilter membrane in extract, to remove albumen, this filter liquor is contacted and the aqueous solution by using an alcohol or alkaline aqueous solution eluting in adsorbate matter of an alcohol go out the candy glycosides with an adsorbent to obtain a filter liquor.
Use the product of microbial fermentation Semen sojae atricolor also to can be used as resistant activity oxygen effect compositions, reagent, food, cosmetics and medicine (for example No. the 4139132nd, Japan Patent).
Yet, there is no prior art and provide or illustrate the low concentration alcohol of the Semen sojae atricolor (Glycine max (L.) Merr.) that is easy to prepare or do not have the purposes of pure steam distillation thing aspect treatment skin injury and dermatosis.
Summary of the invention
The invention provides a steam distillation thing from the acquisition of Semen sojae atricolor (Glycine max (L.) Merr.) seed, it has remarkable effect to treatment skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth.
Steam distillation thing of the present invention prepares by a method that comprises following step:
(a) provide the crude extract of seed in an alcoholic solution (it contains the alcohol that concentration is lower than about 15%wt) or water; And
(b) atmospheric pressure be lower than about 1atm and temperature be lower than about 110 ℃ down the evaporation crude extracts to obtain the steam distillation thing.
This steam distillation thing is different from any known Semen sojae atricolor (Glycine max (L.) Merr.) seed extract, is the generating writing pattern that is shown as a uniqueness because of its fingerprint that sees through high performance liquid chromatography (HPLC).Particularly, using internal diameter (I.D.) is that 4.6 millimeters, length (L) are that 250 millimeters and granular size are 5 microns Waters Sphersorb
TMThe HPLC collection of illustrative plates of the 50 microlitre steam distillation things that-ODS2 tubing string is obtained in 200 nano wave lengths comprise coefficient of deviation be 8% and lag time be 2.910,5.190,13.190 and 50.815 minutes peak value, wherein mobile phase was 95%H at 0 to 5 minute
2O/5%CH
3CN, the gradient at 5 to 30 minutes is 95%H
2O/5%CH
3CN, the gradient at 30 to 80 minutes is 75%H
2O/25%CH
3CN, the gradient at 80 to 100 minutes is 0%H
2O/100%CH
3CN, the gradient at 100 to 101 minutes is 0%H
2O/100%CH
3CN, and be 95%H at 101 minutes
2O/5%CH
3CN; And flow velocity is 1 ml/min.
The present invention also provides a compositions that comprises steam distillation thing of the present invention.Particularly, said composition can be used for a medical composition, cosmetic composition or skin cleansing compositions.
Description of drawings
Fig. 1 shows the HPLC collection of illustrative plates by the fraction 1 that obtains according to the inventive method; Fig. 1 a:0 to 20 minute; Fig. 1 b:20 to 40 minute; Fig. 1 c:40 to 60 minute.
Fig. 2 shows the HPLC collection of illustrative plates by the fraction 3 that obtains according to the inventive method; Fig. 2 a:0 to 20 minute; Fig. 2 b:20 to 40 minute; Fig. 2 c:40 to 60 minute.
Fig. 3 shows the photo that uses in the example 3 according to Ointment in Treatment whole bark layer wound of the present invention.
Fig. 4 shows and uses in the example 3 according to the hair growth photo on the whole bark layer wound of Ointment in Treatment of the present invention; Fig. 4 a: the ointment that comprises fraction 1 to 3; Fig. 4 b: the ointment that comprises fraction 4 to 6.
Fig. 5 shows the histological examination result of untreated deep second degree burn wound in the example 5.
Fig. 6 shows in the example 5 the histological examination result through the deep second degree burn wound of silver sulfadiazine Drug therapy.
Fig. 7 shows the histological examination result who uses in the example 5 according to Ointment in Treatment deep second degree burn wound of the present invention.
Fig. 8 shows in the example 6 the histological examination result through the large tracts of land whole bark layer wound of silver sulfadiazine Drug therapy.
Fig. 9 shows the histological examination result who uses in the example 6 according to Ointment in Treatment large tracts of land whole bark layer wound of the present invention.
Figure 10 shows in the example 7 and uses according to Ointment in Treatment irradiated skin injured tissues check result of the present invention.
Figure 11 shows untreated irradiated skin injured tissues check result in the example 7.
Figure 12 shows the result of normal structure in the example 7.
Figure 13 shows the photo before the rabbit ear dermatitis in treatment in the example 8.
Figure 14 shows in the example 8 and to use according to the 1st day photo after the Ointment in Treatment rabbit ear dermatitis of the present invention.
Figure 15 shows in the example 8 and to use according to the 2nd day photo after the Ointment in Treatment rabbit ear dermatitis of the present invention.
Figure 16 shows in the example 8 and to use according to the 3rd day photo after the Ointment in Treatment rabbit ear dermatitis of the present invention.
Figure 17 shows the photo of diabetic wound in the example 9.
Figure 18 shows the photo of diabetic wound in the example 9.
Figure 19 shows in the example 9 and uses according to the photo of Ointment in Treatment diabetic wound of the present invention after 2 months.
Figure 20 shows in the example 9 and uses according to the photo of Ointment in Treatment diabetic wound of the present invention after 5 months.
Figure 21 shows in the example 9 and uses according to the photo of Ointment in Treatment diabetic wound of the present invention after 5 months.
Figure 22 shows the photo of chronic accumulative ulcer in the example 10.
Figure 23 shows in the example 10 and uses according to the photo of Ointment in Treatment chronic accumulative ulcer of the present invention after 2 weeks.
Figure 24 shows in the example 10 and uses according to the photo of Ointment in Treatment chronic accumulative ulcer of the present invention after 4 weeks.
Figure 25 shows the photo of ecchymosis in the example 11.
Figure 26 shows in the example 11 and uses according to the photo of Ointment in Treatment ecchymosis of the present invention after 3 days.
Figure 27 shows in the example 11 and uses according to the photo of Ointment in Treatment ecchymosis of the present invention after 7 days.
Figure 28 shows abrasive photo in the example 12.
Figure 29 shows in the example 12 and uses according to the photo of Ointment in Treatment ecchymosis of the present invention after 7 days.
Figure 30 shows the photo that uses in the example 13 according to Ointment in Treatment sew up wound of the present invention.
Figure 31 shows the photo of atopic dermatitis in the example 14.
Figure 32 shows in the example 14 and uses according to the photo of Ointment in Treatment atopic dermatitis of the present invention after 1 week.
Figure 33 shows in the example 14 and uses according to the photo of Ointment in Treatment atopic dermatitis of the present invention after 4 weeks.
Figure 34 shows in the example 14 and uses according to the photo of Ointment in Treatment atopic dermatitis of the present invention after 6 weeks.
The specific embodiment
According to the present invention, people find that unexpectedly the steam distillation thing of Semen sojae atricolor (Glycine max (L.) Merr.) has remarkable effect to treatment skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth.
According to the present invention, a steam distillation thing that obtains from Semen sojae atricolor (Glycine max (L.) Merr.) seed prepares by the method that comprises following step:
(a) provide the crude extract of a seed in alcoholic solution (it contains the alcohol that concentration is lower than about 15%wt) or water; And
(b) atmospheric pressure be lower than about 1atm and temperature be lower than about 110 ℃ down these crude extracts of evaporation to obtain the steam distillation thing.
People find can have effect aspect the dermatological from the steam distillation thing that the crude extract of seed in an alcoholic solution (it contains the alcohol that concentration is lower than about 15%wt) or water obtains unexpectedly.This steam distillation thing is easy to preparation.Seed prepares medicine or cosmetics are very favourable from Semen sojae atricolor (Glycine max (L.) Merr.), because: (1) Semen sojae atricolor can eat and it is believed that avirulence for a long time; And (2) Semen sojae atricolor is cheap.
In a specific embodiment of the present invention, the crude extract of Semen sojae atricolor (Glycine max (L.) Merr.) seed obtains by using the extraction of an alcoholic solution (it contains the alcohol that concentration is lower than about 15%wt) or water.Be commonly used in this skill all can be used for implementing the present invention from any method of plant seed acquisition crude extract.For example, this crude extract can for example grind by any traditional method, stirring, disturbance, cutting or chopping method are divided into fragment with seed and it is immersed in the alcoholic solution that a concentration is lower than about 15%wt obtains.In a preferred specific embodiment, determining alcohol is lower than about 3%wt or about 2%wt for being lower than about 10%wt, being preferably.According to the present invention, the also available water extraction of this crude extract.In a preferred specific embodiment, the seed fragment is immersed in by weight in about 5% alcohol or water to about 10% scope.Be commonly used to cut apart seed in this skill and extract this any way of having cut apart seed all can be used for implementing the present invention.
According to the present invention, this crude extract is lower than about 1atm and temperature at atmospheric pressure and is lower than under about 110 ℃ (preferably between about 60 ℃ to about 110 ℃) and is evaporated.This steam distillation thing can be collected with liquid form by cooling off this steam.
In of the present invention one preferred specific embodiment, this crude extract of evaporation and the method for collecting this steam distillation thing by cooling steam can be implemented in a rotary evaporator under a given atmospheric pressure and temperature, wherein steam is evaporated to and supplies to have in the condensing tube of cold water, and makes this steam collect this steam distillation thing by this condensing tube cooling and with liquid form subsequently.This operation is simple and with low cost.
Steam distillation thing according to the present invention can use high performance liquid chromatography (HPLC) to analyze and evaluation.For example, using internal diameter (I.D.) is that 4.6 millimeters, length (L) are that 250 millimeters and granular size are 5 microns Waters Sphersorb
TMThe HPLC collection of illustrative plates of the 50 microlitre steam distillation things that-ODS2 tubing string is obtained in 200 nano wave lengths comprise coefficient of deviation be 8% and lag time be 2.910,5.190,13.190 and 50.815 minutes peak value, wherein mobile phase was 95%H at 0 to 5 minute
2O/5%CH
3CN; Gradient at 5 to 30 minutes is 95%H
2O/5%CH
3CN, the gradient at 30 to 80 minutes is 75%H
2O/25%CH
3CN; Gradient at 80 to 100 minutes is 0%H
2O/100%CH
3CN; Gradient at 100 to 101 minutes is 0%H
2O/100%CH
3CN was 95%H at 101 minutes
2O/5%CH
3CN, and flow velocity is 1 ml/min.According to this collection of illustrative plates, the material in the steam distillation thing shows strong polarity.It obviously is different from and is disclosed in other component of the prior art.For example, there are four rings and its to have low pole in the structure of daidzein and genistein.
In of the present invention one preferred specific embodiment, can add some medical herbs to help Semen sojae atricolor (Glycine max (L.) Merr.) seed treatment skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth.According to the present invention, a steam distillation thing obtains from containing Semen sojae atricolor (Glycine max (L.) Merr.) seed and containing at least a raw material that is selected from the material among the group who is made up of Semen Sesami (Sesamum indicum) seed, Semen phaseoli radiati (Phaseolus radiatus L.) seed and antibiotic medical herbs.Preparation steam distillation thing and analysis and this steam distillation thing of evaluation can be implemented as mentioned above in raw material.Preferably, this antibiotic medical herbs is selected from the group who is made up of the dried bark of the dried bark of the dried rhizome of the dried rhizome of Radix Et Rhizoma Rhei (Rheum palmatum L.), Radix Et Rhizoma Rhei (RheiRhizoma), Cortex Phellodendri (Phellodendron amurense Rupr.) and Cortex Phellodendri (Phellodendri Cortex).On the other hand, preferably comprise about 40% to about 100% Semen sojae atricolor (Glycine max (L.) Merr.) seed, 0 to about 50% Semen Sesami (Sesamum indicum) seed, 0 to about 50% Semen phaseoli radiati (Phaseolus radiatus L.) seed and 0 to about 30% antibiotic medical herbs in the raw material.
In of the present invention one preferred specific embodiment, comprise dried rhizome and the dried bark of about 5% Cortex Phellodendri (Phellodendron amurenseRupr.) or the dried bark of Cortex Phellodendri (Phellodendri Cortex) of the dried rhizome of about 70% Semen sojae atricolor (Glycine max (L.) Merr.) seed, about 20% Semen Sesami (Sesamum indicum) seed, about 5% Radix Et Rhizoma Rhei (Rheum palmatum L.) or Radix Et Rhizoma Rhei (RheiRhizoma) in the raw material.This raw material places about 2% alcohol.
In another preferred specific embodiment of the present invention, comprise about 80% Semen sojae atricolor (Glycine max (L.) Merr.) seed and about 20% Semen phaseoli radiati (Phaseolusradiatus L.) seed in the raw material.This raw material places about 2% alcohol.
In a preferred specific embodiment more of the present invention, comprise about 80% Semen sojae atricolor (Glycine max (L.) Merr.) seed and about 20% Semen Sesami (Sesamumindicum) seed in the raw material.This raw material places about 2% alcohol.
In another preferred specific embodiment of the present invention, comprise the dried rhizome of Radix Et Rhizoma Rhei (Rheum palmatumL.) of about 90% Semen sojae atricolor (Glycine max (L.) Merr.) seed and about 10% or the dried rhizome of Radix Et Rhizoma Rhei (Rhei Rhizoma) in the raw material.This raw material places water.
In another preferred specific embodiment of the present invention, comprise the dried rhizome of Radix Et Rhizoma Rhei (Rheum palmatum L.) of about 80% Semen sojae atricolor (Glycine max (L.) Merr.) seed, about 10% Semen Sesami (Sesamum indicum) seed and about 10% or the dried rhizome of Radix Et Rhizoma Rhei (RheiRhizoma) in the raw material.This raw material places water.
In most preferably specific embodiment of the present invention, comprise dried rhizome and the dried bark of about 5% Cortex Phellodendri (Phellodendron amurense Rupr.) or the dried bark of Cortex Phellodendri (Phellodendri Cortex) of the dried rhizome of about 53% Semen sojae atricolor (Glycine max (L.) Merr.) seed, about 20% Semen Sesami (Sesamum indicum) seed, about 17% Semen phaseoli radiati (Phaseolus radiatus L.) seed, about 5% Radix Et Rhizoma Rhei (Rheum palmatum L.) or Radix Et Rhizoma Rhei (Rhei Rhizoma) in the raw material.This raw material places about 3% or about 2% alcohol or water.And using internal diameter (I.D.) is that 4.6 millimeters, length (L) are that 250 millimeters and granular size are 5 microns Waters Sphersorb
TMThe HPLC collection of illustrative plates of the 50 microlitre steam distillation things that-ODS2 tubing string is obtained in 200 nano wave lengths comprise coefficient of deviation be 8% and lag time be 2.896,5.111,12.944 and 46.603 minutes peak value, wherein mobile phase was 95%H at 0 to 5 minute
2O/5%CH
3CN, the gradient at 5 to 30 minutes is 95%H
2O/5%CH
3CN, the gradient at 30 to 80 minutes is 75%H
2O/25%CH
3CN, the gradient at 80 to 100 minutes is 0%H
2O/100%CH
3CN, the gradient at 100 to 101 minutes is 0%H
2O/100%CH
3CN was 95%H at 101 minutes
2O/5%CH
3CN, and flow velocity is 1 ml/min.
The present invention also provides a compositions that contains with good grounds steam distillation thing of the present invention.Preferably, said composition is selected from by medical composition, cosmetic composition and skin cleansing compositions.In according to of the present invention one preferred specific embodiment, said composition further comprises an antibiotic preparation, for example Borneolum Syntheticum.Preferably, according to the amount of steam distillation thing of the present invention between composition total weight about 70% to about 80% between.
In a specific embodiment of the present invention, said composition further comprises Borneolum Syntheticum, stearic acid, stearyl alcohol, Palmic acid, hexadecanol, Cera Flava and polyoxyethylene sorbitan monostearate (POE) to form an ointment that is easy to impose on a curee.
One is used for the treatment of skin injury or dermopathic method comprises with the q.s that can effectively treat skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth and uses according to compositions of the present invention.
Steam distillation thing according to the present invention can be used for treating skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth.For example, skin injury or dermatosis comprise burn, sunburn, radiational injury, acute and chronic injury, ecchymosis, dermatitis, speckle, pimple, brief summary, vesicle, big vesicle, pustule, rubella wheal, speckle, cyst, squama disease, crust, crack, ulcer, acne, xeroderma, decortication, pruritus, allergic eczema, rash and hair follicle defects.Preferably, can effectively treat burn, radiational injury, acute and chronic injury, dermatitis, gangrene, chronic accumulative ulcer, scratch, atopic dermatitis, sew up wound, diabetic wound and hair follicle defects according to compositions of the present invention.
In following animal model, identity basis steam distillation thing of the present invention is effective to large area skin defective, cicatrix and anaphylaxis that treatment does not contain granulation.In addition, it also can be treated hair follicle defects and hair and can regrow and not stay erythema or eschar.Use uses the conventional medicament therapist preferred according to the recovery speed and the condition of steam distillation thing treatment wound of the present invention than those.Use does not have cicatrix during according to the wound healing of steam distillation thing of the present invention treatment, and the skin color of healing of wound is identical with the normal skin color.In addition, in histological examination, use does not show the fibre modification of the reconstruction operations that can cause huge damage of outward appearance and needs complexity usually according to the skin histology of the wound of steam distillation thing treatment of the present invention.
In addition, use the wound of steam distillation thing treatment of the present invention not find anaphylaxis, inflammation, pustule or rash.After the treatment, the accessory organ of wound and the growth of cell are identical with in the normal skin those.In contrast, the normal skin of the wound that uses a conventional medicament to treat has the accessory organ and the cell of lesser amt, and the fiber width of this wound is bigger.
In addition, can effectively treat radiational injury according to steam distillation thing of the present invention.Up to now, Shang Wuke effectively treats the medicine of radiational injury.People find unexpectedly, suffer among the curee of radiational injury one, use the wound after this steam distillation thing is treated to show lower hair damages and do not have irritated reaction.
In addition, can when wound healing, observed inflammation not come across in the wound of use according to steam distillation thing treatment of the present invention usually with organizing contracture.This steam distillation thing can effectively be treated the wound that skin sustains damage.So, the invention provides preferred, a convenient and economic mode that is used for the treatment of large tracts of land and skin wound.
Following example only is used to explain purpose, but not scope of the present invention is limited.
Example 1: the system of the steam distillation thing of Semen sojae atricolor (Glycine max (L.) Merr.) seed
Be equipped with
Grinding comprises Semen sojae atricolor (Glycine max (L.) Merr.) seed, Semen Sesami (Sesamumindicum) seed, Semen phaseoli radiati (Phaseolus radiatus L.) seed, the raw material of dried bark of the dried rhizome of the dried rhizome of Radix Et Rhizoma Rhei (Rheumpalmatum L.) or Radix Et Rhizoma Rhei (Rhei Rhizoma) and Cortex Phellodendri (Phellodendron amurense Rupr.) or the dried bark of Cortex Phellodendri (PhellodendriCortex.) and use by weight 8% alcohol or the distilled water extraction to obtain a crude extract.Its content is as shown in table 1.The steam distillation thing by be lower than at pressure 1atm and temperature be under 60 ℃ in a rotary evaporator (EYELA N-1000S, 1000S-W) in this crude extract of evaporation and it is obtained for the condensing tube that cold water is arranged by.
Table 1:
The HPLC result of 50 microlitre fractions 1 as shown in Figure 1.This HPLC is that 4.6 millimeters, length (L) are that 250 millimeters and granular size are 5 microns Waters Sphersorb by using internal diameter (I.D.)
TM-ODS2 tubing string is implemented, and wherein mobile phase was 95%H at 0 to 5 minute
2O/5%CH
3CN, the gradient at 5 to 30 minutes is 95%H
2O/5%CH
3CN, the gradient at 30 to 80 minutes is 75%H
2O/25%CH
3CN, the gradient at 80 to 100 minutes is 0%H
2O/100%CH
3CN, the gradient at 100 to 101 minutes is 0%H
2O/100%CH
3CN was 95%H at 101 minutes
2O/5%CH
3CN, and flow velocity is 1 ml/min.This HPLC uses Waters Alliance 2795HT to implement, and this collection of illustrative plates uses Waters Photo Diode Array (photodiode array) 2996 in 190 to 400 nano wave lengths to obtain.Shown in the collection of illustrative plates of obtaining in 200 nano wave lengths, coefficient of deviation be 8% and lag time have spike when being 2.910,5.190,13.190 and 50.815 minutes.
The HPLC result of 50 microlitre fractions 3 as shown in Figure 2.This HPLC is that 4.6 millimeters, length (L) are that 250 millimeters and granular size are 5 microns Waters Sphersorb by using internal diameter (I.D.)
TM-ODS2 tubing string is implemented, and wherein mobile phase was 95%H at 0 to 5 minute
2O/5%CH
3CN, the gradient at 5 to 30 minutes is 95%H
2O/5%CH
3CN, the gradient at 30 to 80 minutes is 75%H
2O/25%CH
3CN, the gradient at 80 to 100 minutes is 0%H
2O/100%CH
3CN, the gradient at 100 to 101 minutes is 0%H
2O/100%CH
3CN was 95%H at 101 minutes
2O/5%CH
3CN, and flow velocity is 1 ml/min.This HPLC uses Waters Alliance 2795 HT to implement, and this collection of illustrative plates uses Waters Photo Diode Array (photodiode array) 2996 in 190 to 400 nano wave lengths to obtain.Shown in the collection of illustrative plates of obtaining in 200 nano wave lengths, coefficient of deviation be 8% and lag time have spike when being 2.896,5.111,12.944 and 46.603 minutes.
Example 2: the preparation of ointment
According to ointment of the present invention such as table 2 illustration:
Table 2:
| Content (wt) | |
| Fraction | 77％ |
| Borneolum Syntheticum | 0.6 |
| Stearic acid | |
| 5％ | |
| Stearyl alcohol | 4％ |
| Palmic acid | 4 |
| Hexadecanol | |
| 5 | |
| Cera Flava | |
| 2％ | |
| Polyoxyethylene sorbitan monostearate | 2.4％ |
Ointment of the present invention can use any traditional method preparation used usually in this skill.
Example 3: to the effect of rabbit whole bark layer wound
Treatment to animal:
Get 5 heavy New Zealand of 2.9 to 3.1 kilograms and grow up female white rabbit as animal model.Every rabbit encaged separately and feed with mixed fodder and water.Use 25 to 40 milligrams of/kilogram ketalar anesthesia rabbits.The back of every rabbit is through thorough cropping and cut off 6 area whole bark layers (wherein each area is 1.5 * 1.5 square centimeters), and wherein the whole bark layer comprises epidermis, corium and sarcolemma.
Dosage and frequency:
6 area wounds of every rabbit use the Ointment in Treatment that comprises fraction 1 to 6, twice of every day respectively.This ointment imposes on the wound with the amount part of 0.35 gram/square centimeter.
Clinical wound evaluation:
Observe wound every day and under an even illumination, focal length and aperture condition, use on every Wendesdays time a reference standard to take pictures with the record wound healing process.In addition, wound size is also through measurement and as shown in table 3.
Table 3:
The result shows that wound heals (as shown in Figure 3) fully.Also effective according to excipient of the present invention (ointment that comprises fraction 5) to treatment whole bark layer wound.
Hair growth is estimated:
Measure the length of new hair.The hair of all wounds that use comprises the Ointment in Treatment of fraction 1 to 6 on average is about 1.76 centimeters (as shown in Figure 4), and the hair in those untreated areas on average length be 0.1 to 0.2 centimeter.
The result shows, can the damaged and stimulating hair growth of repairing hair follicle according to steam distillation thing of the present invention.In addition, also effective according to excipient of the present invention to strengthening hair growth.
Example 4: to the effect of rat whole bark layer wound
Treatment to animal:
Buy Sprague-Dawley bull rat from the Experimental Animal Center of Taiwan National Chenggong University.Use 25 to 40 milligrams of/kilogram ketalars to anaesthetize the rat of heavy 300 to 350 grams.The thorough cropping of electric clipper is all used at the back of every rat.Every rat encaged separately and feed with mixed fodder and water.For every rat, cut off an area and be 2.5 * 2.5 square centimeters whole bark layer, wherein this whole bark layer comprises epidermis, corium and sarcolemma, referring to " using ossein/elastin laminin-film to make up dermal tissue " (Use of a collagen/elastin-membrane for the tissueengineering of dermis.Burns.1999 of people such as B.Hafemann; 25:373-384) and " using a pig dermis template to strengthen the establiss of the thin layer of extensive expansion mesh " (Use of a Porcine Dermis Template to EnhanceWidely Expanded Mesh Autologus Split-Thickness Skin GraftGrowth.J Trauma.1997 Feb of people such as H.-J.Wang from body half thick skin; 42 (2): 177-182).
Dosage and frequency:
The Ointment in Treatment that comprise fraction 7 and the 8 two experimental group rats of use shown in example 2, and every group comprise 3 rats.This medical composition imposes on the wound with the amount of 0.35 gram/square centimeter and 0.1 to 0.2 centimeter thickness part.All wounds are all wrapped up with antiseptic gauze and elastic bandage, and all change dressings every day.
Used evaluation methodology is similar to method used in the example 3, and wound size is also also as shown in table 4 through measuring.
Table 4:
Use comprises the wound healing of the Ointment in Treatment of fraction 7 and 8.In specific words, use the wound area of the medical composition treatment that comprises fraction 7 to dwindle sooner.
Example 5: to the effect of skin deep second degree burn wound healing
Treatment to animal:
Buy the adult female rats of Sprague-Dawley from the Experimental Animal Center of Taiwan National Chenggong University.Use the rat of 9 heavy 230 to 280 grams of 25 to 40 milligrams of/kilogram ketalar anesthesia.In every rat back intermediary one predetermined wound area, all use the thorough cropping of electric clipper.Every rat encaged separately and feed with mixed fodder and water.Use as Walker and the described method of Mason and produce the rats after burn wound, referring to " the Corii Sus domestica ossein is used for skin donors position and skin deep second degree burn as the wound dressing articles for use " (Porcine dermal collagen as a wound dressingfor skin donor sites and deep partial skin thickness burns.Burns.1992 of people such as Z-R.Gao; 18 (6): 492-496), wherein skin of back surface sees through one and is designed for that to produce area be that the template of 4 * 4 square centimeters skin deep second degree burn is exposed to 15 seconds in 75 ℃ of water.
Dosage and frequency:
Rat experiment group (G3) is used according to medical composition treatment of the present invention, and it comprises the fraction 9 that is similar to fraction 3, and its difference is that the content of alcohol is 3% shown in the example 2.Rat matched group (G1) untreated and rat comparable group (G2) are through the treatment of silver sulfadiazine medicine (by SWISS PHARMACEUTICAL CO., LTD. produces).Every group comprises 3 rats.This medicine imposes on the wound with the amount part of 0.35 gram/square centimeter, and every day twice is till hair grows.
Clinical wound evaluation:
Used evaluation methodology is similar to those used in the example 3 methods, and wound size is also as shown in table 5 through measuring.
Table 5:
In wound healing process, do not have cicatrix when using the wound healing according to Ointment in Treatment of the present invention, and its skin color is identical with the normal skin color.Yet, on the wound of use silver sulfadiazine Drug therapy cicatrization is arranged.In addition, the wound of rat healed in the time of the 18th day, and it uses the rat (its still surplus the 37th day the time 2.86% wound area) of traditional silver sulfadiazine Drug therapy early than those.
The new hair growth fraction is also through estimating and record.The new hair growth fraction is defined as follows: " 2 " refer to not have the new hair growth, and have some eschars and erythema; " 1 " refers to not have the new hair growth, and has some erythema; " 0 " refers to not have the new hair growth, and has normal skin; "+1 " refers to some new hair growths; And "+2 " refer to the new hair growth of about 50% wound area.
The new hair growth fraction is listed in the table 6:
Table 6
As shown in table 6, the more traditional silver sulfadiazine medicine of ointment according to the present invention is more effective to the new hair growth.When using medical composition according to the present invention to treat the 18th day, observe the new hair growth, and in the time of the 35th day, observe 50% new hair growth.In the wound that uses according to medical composition treatment of the present invention, do not find erythema and eschar.Yet, when using the conventional medicament treatment, do not observe the new hair growth.
The Histological evaluation of skin:
After wound thoroughly heals, get the skin histology of every rat and its anesthesia is implemented histological examination to use as the described evaluation methodology of people such as B.Hafemann, referring to " using ossein/elastin laminin-film to make up dermal tissue " (Use ofa collagen/elastin-membrane for the tissue engineering ofdermis.Burns.1999 of people such as B.Hafemann; 25:373-384).Take out sample and it is stored in the formaldehyde.Be embedded in the paraffin sealing each sample and the preparation slice, and use the dyeing of hematoxylin-eosin method.Observation sample under an optical microscope and a transmission electron microscope.
Histological examination is the result show, untreated rat epithelial tissue (as shown in Figure 5) in uneven thickness, and granular layer thickening.Fibrous layer can be observed in dark cortex.Use in the rat group (as shown in Figure 6) of traditional silver sulfadiazine Drug therapy and also show to have epithelial tissue in uneven thickness.Fibrous layer can be observed in dark cortex, and hair follicle is damaged.On the other hand, use according to the wound (as shown in Figure 7) of Ointment in Treatment of the present invention to it seems it is normal, and a large amount of hair follicle regeneration.Fibrous layer can be observed at skin surface area.
Example 6: to the effect of large tracts of land whole bark layer wound in the rat
Treatment to animal:
Buy Sprague-Dawley bull rat from the Experimental Animal Center of Taiwan National Chenggong University.Use 25 to 40 milligrams of/kilogram ketalars to anaesthetize the rat of heavy 300 to 350 grams.The thorough cropping of electric clipper is all used at the back of every rat.Every rat encaged separately and feed with mixed fodder and water.For every rat, cut off an area and be 4.0 * 4.0 square centimeters whole bark layer, wherein this whole bark layer comprises epidermis, corium and sarcolemma.
Dosage and frequency:
The experimental group of rat uses the Ointment in Treatment that comprises the fraction 9 as shown in example 5.The present invention also provides a rat comparable group (G2) of using silver sulfadiazine medicine (by SWISS PHARMACEUTICALCO., LTD. produces) to treat.Every group contains 5 rats.This ointment imposes on the wound with the amount of 0.75 gram/square centimeter and 0.1 to 0.2 centimeter thickness part.All wounds are all wrapped up with antiseptic gauze and elastic bandage, and all change dressings every day.
Used evaluation methodology is similar to those used in the example 3 methods, and wound size is also also as shown in table 7 through measuring.
Table 7:
In the wound healing process, use according to the wound of Ointment in Treatment of the present invention and can cure and not have irritated reaction.Yet, can form cicatrix on the wound of use silver sulfadiazine Drug therapy.In addition, in the wound that uses the silver sulfadiazine Drug therapy, can be observed severe anaphylactic reaction.Use is used the rat (about 37 days) of silver sulfadiazine Drug therapy according to the recovery speed (about 25 to 31 days) of the rat of medical composition treatment of the present invention faster than those.
The Histological evaluation of skin:
Be used for described in the method such as example 5 of Histological evaluation.
The histological examination result who implemented at the 45th day shows, can observe fibrous layer in the dark cortex of wound that uses traditional silver sulfadiazine Drug therapy.Some epithelial cell death and formation is crust more.In skin, can be observed chronic inflammatory disease and eosinophil is permeated the two.Some fiber also can be formed at apart from 0.5 centimeter place of wound.
In the rat group of using according to Ointment in Treatment of the present invention, new epithelize is smooth, and a large amount of hair follicle produces.Only form small amount of fibers.
The histological examination result who implemented at the 95th day shows, can observe inhomogeneous epithelial layer (as shown in Figure 8) on the wound that uses traditional silver sulfadiazine Drug therapy.Fibrous layer is formed in the dark cortex.A small amount of hair follicle produces.
In the rat group of using according to Ointment in Treatment of the present invention, new epithelize is smooth, and a large amount of hair follicle produces (as shown in Figure 9).
Example 7: to the effect of local electron radiation skin injury
Treatment to animal:
Buy the adult female rats of Sprague-Dawley from the Experimental Animal Center of Taiwan National Chenggong University.The rats of heavy 250 to 300 grams are encaged separately and feed with mixed fodder and water.
Rat is similar to as John E.Moulder and the described method of James J.Fischer by use and implements radiation, referring to " radioreaction of rat skin: the effect of fraction quantity and total treatment time " (RADIATION REACTION OF RAT SKIN:The roleof the Number of Fractions and the Overall Treatment Time.Cancer June 1976; 37 (6): 2762-2767).In rat peritoneum, use 40 to 50 milligrams of/kilogram sodium pentobarbitals (TCI.TOKYO) to come anesthetized rat before the radiation.Cut the skin on the gluteus zone fully, and before radiation, on the right side of 2 * 2 square centimeters of swept areas of expection and left side gluteus, mark.The electron beam that applies the 6MeV energy that produces by a linear accelerator (VarianAssociates, Inc., CA, U.S.A. system) is implemented irradiation.
Test gradation and single dose irradiation.In fractionation of radiation, right side of 3 rats and gluteus zone, left side are shone.This irradiation implements twice weekly, implements for five weeks, and the accumulated dose on each zone is 37.5Gy.
Dosage and frequency:
In fractionation of radiation, use the Ointment in Treatment that comprises the fraction 9 (G1) as shown in example 5, and the radiational injury in the single dose irradiation is implemented two treatments every day with the radiational injury after the dosage every day of the irradiation once of 0.25 gram/square centimeter.Untreated rat is with comparing (G2).The blank group of rat (G3) of not accepting irradiation is as blank.
Clinical evaluation:
Estimate the also back 30 days dermoreaction mark of record irradiation, referring to " the part side dependency acute skin reaction of mice after the irradiation of two doses every day repeatedly " (Fraction side-dependent Acute SkinReaction of Wice after Multiple Twice-A-Day Doses.Int.J.Radiation Oncology Biol.Phys.1990 Feb of Yoshinao Abe and Muneyasu Urano; 18 (2): 359-364) and " tetrandrine and Gotu Kola extract are to the effect of acute radiation dermatitis in the rat " (The Effect of Tetrandrine and Extracts ofCentella asiatica on Acute Radiation Dermatitis in Rats.Biol.Pharm.Bull.1999 July of people such as Yu-Jen Chen; 22 (7): 703-706).Dermoreaction is fractional to be defined as follows: " 0 " the normal skin of making a comment or criticism; " 0.5 " refer to be less than 50% through irradiation area slight depilation appears, or hair variable color; " 1.0 " refer to that depilation appears in about 50% irradiation area; " 1.5 " refer to follow skin rubefaction above irradiation area appearance depilation and some depilation of 50%; " 2.0 " refer to be less than 50% irradiation area appearance depilation and dried decortication fully; " 2.5 " refer to surpass 50% the dried decortication of irradiation area appearance; " 3.0 " point out existing some wet decortication; " 3.5 " refer to that wet decortication appears in most of zone.
This dermoreaction mark is through evaluation and as shown in table 8.
Table 8:
In G1, find early stage cut and a small amount of hair damages take place in the wound, and skin does not show anaphylaxis.
This result is similar to the fractionation of radiation test result.
The Histological evaluation of skin:
Used evaluation methodology is similar to those used in the example 5 methods.
(as shown in figure 10) table of discovery chrotoplast is covered on the multilamellar epithelial cell in G1.Also there are a large amount of hair follicle regenerations.
In G2 (as shown in figure 11), only observe a small amount of hair follicle.
In G3 (as shown in figure 12), this tissue in contrast.
Wound tissue through treatment is fine and close, but those untreated wounds are more lax.
Example 8: to the effect of rabbit ear dermatitis
As shown in figure 13, it is found that rabbit ear dermatitis has hyperemia, swelling, inflammation, ulcer and bleeding.
Wound is used 3 days according to ointment of the present invention, and it comprises the fraction 10 that is similar to fraction 3, and difference is that pure content wherein is 0% shown in the example 2.
At the 1st day, ulcer disappeared and hyperemia alleviates.Hair follicle occurs, as shown in figure 14.
At the 2nd day, skin histology recovered and only observes slightly rubescent.Hair follicle obviously as seen, as shown in figure 15.
At the 3rd day, skin histology and color were normal.Hair is grown on hair follicle, as shown in figure 16.
Example 9: to the effect of diabetic wound
The male suffered from diabetes 20 years in one 68 years old.Its left foot toe excises.Wound shows that serious gangrene changes, as shown in figure 17.Whole toes of its right crus of diaphragm are cut but wound healing is not good, as shown in figure 18.
The ointment that will comprise fraction 9 imposes on wound, every day 2 to 3 times.
Left foot can be noticed some granulation tissue after treatment in 2 months (as shown in figure 19), and wound begins healing and more downright badly do not have.
Left foot is after treatment in 5 months (as shown in figure 20), and wound heals and preventing from scar fully.Right crus of diaphragm (as shown in figure 21) also heals and preventing from scar fully.
Example 10: to the effect of chronic accumulative ulcer
As shown in figure 22, one 80 years old male's both sides lower limb trouble hyperpigmentation and the long-pending ulcer of the stasis of blood are 20 years.
The ointment that will comprise fraction 9 imposes on the wound, every day 2 to 3 times.
Foot is after the treatment of 2 weeks (as shown in figure 23), and wound begins healing.
Foot is after the treatment of 4 weeks (as shown in figure 24), and wound heals and preventing from scar fully.
Example 11: to the effect of ecchymosis
One female patient suffers from the ecchymosis (as shown in figure 25) that traffic accident causes.
Use every day a cold compress that this wound is treated three days in advance, and use the ointment that comprises fraction 92 to 3 times every day subsequently.
As shown in figure 26, ecchymosis disappears and wound healing in the 3rd day after treatment.Already present cicatrix and color also flatten and shoal (as shown in figure 27) after 7 days in treatment before the traffic accident.
Example 12: to abrasive effect
As shown in figure 28, a female patient suffers from the skin abrasion that traffic accident causes.
The ointment that will comprise fraction 9 imposes on the wound, every day 2 to 3 times.
Wound is in treatment healing (as shown in figure 29) after 7 days.
Example 13: to the effect of sew up wound
One male patient suffers from and seriously tears wound, has on its philtrum to have on 3 centimeters breaches and 20 pin sutures, the bridge of the nose respectively to have 1 centimeter breach and 6 pin sutures and 1.5 centimeters breaches and 8 pin sutures on 0.8 centimeter breach and 4 pin sutures and the upper lip.
The ointment that will comprise fraction 9 imposes on the wound, every day 2 to 3 times.
Wound heals fully and preventing from scar (as shown in figure 30).
Example 14: to the effect of atopic dermatitis
The young girl suffered from chronic atopic dermatitis in one 4.5 years old.As shown in figure 31, after long-term topical corticosteroid treatment, its cervical region and the skin of going up breast are itched, hyperemia, swelling, split, ooze out, form a scab and squama is arranged.
The ointment that will comprise fraction 10 imposes on the wound, every day 2 to 3 times.
Through 1 week treatment back (shown in figure 32), itch, hyperemia, swelling, split, ooze out, incrustation and squama minimizing and weaken.
After the treatment of 4 weeks (as shown in figure 33), skin injury almost disappears.
After the treatment of 6 weeks (as shown in figure 34), skin injury heals fully and does not have rebound effect subsequently.
Although explained and described specific embodiments of the invention, those of ordinary skill of the present invention still can be implemented various modifications and improvement.Therefore, specific embodiments of the invention only are used to the purpose of explaining but not the present invention are limited.The present invention is not limited to the particular form set forth, and modifies all in the defined scope of the claim of enclosing without prejudice to all of purport of the present invention and scope.
Claims (46)
1. steam distillation thing that obtains from the Semen sojae atricolor seed, this steam distillation thing are by the method preparation that comprises following step:
(a) provide this seed to be lower than the alcoholic solution of 15%wt alcohol or the crude extract in the water containing concentration; And
(b) atmospheric pressure be lower than 1atm and temperature be lower than 110 ℃ down these crude extracts of evaporation to obtain this steam distillation thing;
Wherein using internal diameter is that 4.6 millimeters, length are that 250 millimeters and granular size are 5 microns Waters Sphersorb
TMThe collection of illustrative plates of the 50 microlitre steam distillation things that-ODS2 tubing string is obtained in 200 nano wave lengths by high performance liquid chromatography comprise coefficient of deviation be 8% and lag time be 2.910,5.190,13.190 and 50.815 minutes peak value, wherein mobile phase was 95%H at 0 to 5 minute
2O/5% CH
3CN, the gradient at 5 to 30 minutes is 95% H
2O/5%CH
3CN, the gradient at 30 to 80 minutes is 75% H
2O/25% CH
3CN, the gradient at 80 to 100 minutes is 0% H
2O/100% CH
3CN, the gradient at 100 to 101 minutes is 0%H
2O/100% CH
3CN was 95% H at 101 minutes
2O/5% CH
3CN, and flow velocity is 1 ml/min.
2. steam distillation thing according to claim 1, wherein this steam distillation thing is collected with liquid form by cooling steam.
3. steam distillation thing according to claim 1, concentration that wherein should alcohol is lower than 5%wt.
4. steam distillation thing according to claim 1, wherein the crude extract in the step (a) places water.
5. steam distillation thing according to claim 1, wherein the temperature in the step (b) is between 60 ℃ to 110 ℃.
6. steam distillation thing according to claim 1, it has the effect of treatment skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth.
7. compositions that comprises steam distillation thing according to claim 1.
8. a compositions according to claim 7 is used to prepare the purposes for the treatment of skin injury or dermatosis treating medicine.
9. purposes according to claim 8, wherein this skin injury or dermatosis are selected from by burn, radiational injury, acute and group that chronic injury, ecchymosis, dermatitis, ulcer, chronic accumulative ulcer, scratch, atopic dermatitis, sew up wound, diabetic wound and hair follicle defects are formed.
10. purposes according to claim 8, wherein this skin injury or dermatosis are selected from burn, radiational injury, acute and chronic injury, dermatitis, gangrene, chronic accumulative ulcer, scratch, atopic dermatitis, sew up wound, diabetic wound and hair follicle defects.
11. compositions according to claim 7, it further comprises an antibacterial.
12. compositions according to claim 7, it further comprises Borneolum Syntheticum, stearic acid, stearyl alcohol, Palmic acid, hexadecanol, Cera Flava and polyoxyethylene sorbitan monostearate.
13. one kind is used for the treatment of patient skin damage or dermopathic medical composition, it comprises the described compositions of claim 7 of the q.s that can effectively treat skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth.
14. the steam distillation thing that obtains in the raw material of self-contained soybean seed and at least a material that is selected from Semen Sesami seed, mung bean seed and antibiotic medical herbs, it is by the method preparation that comprises following step:
(a) provide this raw material to contain concentration and be lower than the alcoholic solution of 15%wt alcohol or the crude extract in the water one; And
(b) atmospheric pressure be lower than 1atm and temperature be lower than 110 ℃ down these crude extracts of evaporation to obtain this steam distillation thing;
Wherein using internal diameter is that 4.6 millimeters, length are that 250 millimeters and granular size are 5 microns Waters Sphersorb
TMThe collection of illustrative plates of the 50 microlitre steam distillation things that-ODS2 tubing string is obtained in 200 nano wave lengths by high performance liquid chromatography comprise coefficient of deviation be 8% and lag time be 2.910,5.190,13.190 and 50.815 minutes peak value, wherein mobile phase was 95% H at 0 to 5 minute
2O/5% CH
3CN, the gradient at 5 to 30 minutes is 95% H
2O/5%CH
3CN, the gradient at 30 to 80 minutes is 75% H
2O/25% CH
3CN, the gradient at 80 to 100 minutes is 0% H
2O/100% CH
3CN, the gradient at 100 to 101 minutes is 0%H
2O/100% CH
3CN was 95% H at 101 minutes
2O/5% CH
3CN, and flow velocity is 1 ml/min.
15. steam distillation thing according to claim 14, wherein this antibiotic medical herbs is selected from the dried rhizome of Radix Et Rhizoma Rhei, the dried rhizome of Radix Et Rhizoma Rhei, the dried bark of Cortex Phellodendri and the dried bark of Cortex Phellodendri.
16. one kind contains the steam distillation thing that obtains in the antibiotic medical herbs of 40% to 100% soybean seed, 0 to 50% Semen Sesami seed, 0 to 50% mung bean seed and 0 to 30% from raw material, it is by the method preparation that comprises following step:
(a) provide this raw material to contain concentration and be lower than the alcoholic solution of 15%wt alcohol or the crude extract in the water one; And
(b) atmospheric pressure be lower than 1atm and temperature be lower than 110 ℃ down these crude extracts of evaporation to obtain this steam distillation thing;
Wherein using internal diameter is that 4.6 millimeters, length are that 250 millimeters and granular size are 5 microns Waters Sphersorb
TMThe collection of illustrative plates of the 50 microlitre steam distillation things that-ODS2 tubing string is obtained in 200 nano wave lengths by high performance liquid chromatography comprise coefficient of deviation be 8% and lag time be 2.910,5.190,13.190 and 50.815 minutes peak value, wherein mobile phase was 95% H at 0 to 5 minute
2O/5% CH
3CN, the gradient at 5 to 30 minutes is 95% H
2O/5%CH
3CN, the gradient at 30 to 80 minutes is 75% H
2O/25% CH
3CN, the gradient at 80 to 100 minutes is 0% H
2O/100% CH
3CN, the gradient at 100 to 101 minutes is 0%H
2O/100% CH
3CN was 95% H at 101 minutes
2O/5% CH
3CN, and flow velocity is 1 ml/min.
17. steam distillation thing according to claim 14, wherein this steam distillation thing is collected with liquid form by cooling steam.
18. steam distillation thing according to claim 14, concentration that wherein should alcohol is lower than 5%wt.
19. steam distillation thing according to claim 14, wherein the crude extract in the step (a) places water.
20. steam distillation thing according to claim 14, wherein the temperature in the step (b) is between 60 ℃ to 110 ℃.
21. steam distillation thing according to claim 14, it has the effect of treatment skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth.
22. compositions that comprises steam distillation thing according to claim 14.
23. a compositions as claimed in claim 22 is used to prepare the purposes of treatment skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth medicine.
24. purposes according to claim 23, wherein this skin injury or dermatosis are selected from burn, radiational injury, acute and chronic injury, ecchymosis, dermatitis, ulcer, chronic accumulative ulcer, scratch, atopic dermatitis, sew up wound, diabetic wound and hair follicle defects.
25. purposes according to claim 23, wherein this skin injury or dermatosis are selected from burn, radiational injury, acute and chronic injury, dermatitis, gangrene, chronic accumulative ulcer, scratch, atopic dermatitis, sew up wound, diabetic wound and hair follicle defects.
26. compositions according to claim 22, it further comprises an antibacterial.
27. compositions according to claim 22, it further comprises Borneolum Syntheticum, stearic acid, stearyl alcohol, Palmic acid, hexadecanol, Cera Flava and polyoxyethylene sorbitan monostearate.
28. one kind is used for the treatment of patient skin damage or dermopathic medical composition, it comprises the described compositions of claim 22 of the q.s that can effectively treat skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth.
29. one kind is used for the treatment of patient skin damage or dermopathic medical composition, it comprises the compositions of the q.s that can effectively treat skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth, said composition comprises the steam distillation thing, and its self-contained following raw material obtains: dried bark of the Cortex Phellodendri of the dried rhizome of 70% soybean seed, 20% Semen Sesami seed, 5% Radix Et Rhizoma Rhei or the dried rhizome of Radix Et Rhizoma Rhei and 5% or the dried bark of Cortex Phellodendri; Wherein this steam distillation thing is prepared by the method that comprises following step:
(a) provide the crude extract of this raw material in 2% alcohol; And
(b) atmospheric pressure be lower than 1atm and temperature be lower than 110 ℃ down these crude extracts of evaporation to obtain this steam distillation thing.
30. medical composition according to claim 29, wherein said composition further comprises Borneolum Syntheticum, stearic acid, stearyl alcohol, Palmic acid, hexadecanol, Cera Flava and polyoxyethylene sorbitan monostearate.
31. the steam distillation thing that obtains in the self-contained following raw material: dried bark of the Cortex Phellodendri of the dried rhizome of 53% soybean seed, 20% Semen Sesami seed, 17% mung bean seed, 5% Radix Et Rhizoma Rhei or the dried rhizome of Radix Et Rhizoma Rhei and 5% or the dried bark of Cortex Phellodendri, this steam distillation thing can effectively be treated skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth when using q.s; Wherein this steam distillation thing is by comprising the method preparation of following step:
(a) provide the crude extract of this raw material in one 2% alcohol; And
(b) atmospheric pressure be lower than 1atm and temperature be lower than 110 ℃ down these crude extracts of evaporation to obtain this steam distillation thing;
Wherein using internal diameter is that 4.6 millimeters, length are that 250 millimeters and granular size are 5 microns Waters Sphersorb
TMThe collection of illustrative plates of the 50 microlitre steam distillation things that-ODS2 tubing string is obtained in 200 nano wave lengths by high performance liquid chromatography comprise coefficient of deviation be 8% and lag time be 2.896,5.111,12.944 and 46.603 minutes peak value, wherein mobile phase was 95% H at 0 to 5 minute
2O/5% CH
3CN, the gradient at 5 to 30 minutes is 95% H
2O/5%CH
3CN, the gradient at 30 to 80 minutes is 75% H
2O/25% CH
3CN, the gradient at 80 to 100 minutes is 0% H
2O/100% CH
3CN, the gradient at 100 to 101 minutes is 0%H
2O/100% CH
3CN was 95% H at 101 minutes
2O/5% CH
3CN, and flow velocity is 1 ml/min.
32. compositions that comprises steam distillation thing according to claim 31.
33. a compositions according to claim 32 is used to prepare the purposes of treatment skin injury or dermatosis treating medicine.
34. purposes according to claim 33, wherein this skin injury or dermatosis are selected from by burn, radiational injury, acute and group that chronic injury, ecchymosis, dermatitis, ulcer, chronic accumulative ulcer, scratch, atopic dermatitis, sew up wound, diabetic wound and hair follicle defects are formed.
35. purposes according to claim 33, wherein this skin injury or dermatosis are selected from burn, radiational injury, acute and chronic injury, dermatitis, gangrene, chronic accumulative ulcer, scratch, atopic dermatitis, sew up wound, diabetic wound and hair follicle defects.
36. compositions according to claim 32, it further comprises an antibacterial.
37. compositions according to claim 32, it further comprises Borneolum Syntheticum, stearic acid, stearyl alcohol, Palmic acid, hexadecanol, Cera Flava and polyoxyethylene sorbitan monostearate.
38. one kind is used for the treatment of patient skin damage or dermopathic medical composition, it comprises the compositions according to claim 32 of the q.s that can effectively treat skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth.
39. one kind is used for the treatment of patient skin damage or dermopathic medical composition, it comprises the compositions of the q.s that can effectively treat skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth, said composition comprises a steam distillation thing, and the raw material of its self-contained 80% soybean seed and 20% mung bean seed obtains; Wherein this steam distillation thing is by comprising the method preparation of following step:
(a) provide the crude extract of this raw material in one 2% alcohol; And
(b) atmospheric pressure be lower than 1atm and temperature be lower than 110 ℃ down these crude extracts of evaporation to obtain this steam distillation thing.
40. according to the described medical composition of claim 39, wherein said composition further comprises Borneolum Syntheticum, stearic acid, stearyl alcohol, Palmic acid, hexadecanol, Cera Flava and polyoxyethylene sorbitan monostearate.
41. one kind is used for the treatment of patient skin damage or dermopathic medical composition, it comprises the compositions of the q.s that can effectively treat skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth, said composition comprises the steam distillation thing, and the raw material of its self-contained 80% soybean seed and 20% Semen Sesami seed makes; Wherein this steam distillation thing is by comprising the method preparation of following step:
(a) provide the crude extract of this raw material in 2% alcohol; And
(b) be lower than the 1atm temperature at atmospheric pressure and be lower than 110 ℃ of following these crude extracts of evaporation to obtain this steam distillation thing.
42. according to the described medical composition of claim 41, wherein said composition further comprises Borneolum Syntheticum, stearic acid, stearyl alcohol, Palmic acid, hexadecanol, Cera Flava and polyoxyethylene sorbitan monostearate.
43. one kind is used for the treatment of patient skin damage or dermopathic medical composition, it comprises the compositions of the q.s that can effectively treat skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth, said composition comprises a steam distillation thing, and the raw material of the dried rhizome of its self-contained 90% soybean seed and 10% Radix Et Rhizoma Rhei or the dried rhizome of Radix Et Rhizoma Rhei obtains; Wherein this steam distillation thing is by containing the method preparation of following step:
(a) provide the crude extract of this raw material in one 2% alcohol; And
(b) atmospheric pressure be lower than 1atm and temperature be lower than 110 ℃ down these crude extracts of evaporation to obtain this steam distillation thing.
44. according to the described medical composition of claim 43, wherein said composition further comprises Borneolum Syntheticum, stearic acid, stearyl alcohol, Palmic acid, hexadecanol, Cera Flava and polyoxyethylene sorbitan monostearate.
45. one kind is used for the treatment of patient skin damage or dermopathic medical composition, it comprises the compositions of the q.s that can effectively treat skin injury, treatment dermatosis, irritation cell regeneration and stimulating hair growth, said composition comprises a steam distillation thing, and its self-contained following raw material obtains: the dried rhizome of 80% soybean seed, 10% Semen Sesami seed and 10% Radix Et Rhizoma Rhei or the dried rhizome of Radix Et Rhizoma Rhei; Wherein this steam distillation thing is by comprising the method preparation of following step:
(a) provide the crude extract of this raw material in one 2% alcohol; And
(b) atmospheric pressure be lower than 1atm and temperature be lower than 110 ℃ down these crude extracts of evaporation to obtain this steam distillation thing.
46. according to the described medical composition of claim 45, wherein said composition further comprises Borneolum Syntheticum, stearic acid, stearyl alcohol, Palmic acid, hexadecanol, Cera Flava and polyoxyethylene sorbitan monostearate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101005964A CN100411638C (en) | 2003-10-20 | 2003-10-20 | Steam distillate from soybean and its composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101005964A CN100411638C (en) | 2003-10-20 | 2003-10-20 | Steam distillate from soybean and its composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1608632A CN1608632A (en) | 2005-04-27 |
| CN100411638C true CN100411638C (en) | 2008-08-20 |
Family
ID=34756018
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2003101005964A Expired - Lifetime CN100411638C (en) | 2003-10-20 | 2003-10-20 | Steam distillate from soybean and its composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100411638C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107456477A (en) * | 2016-06-06 | 2017-12-12 | 侪升生化技术股份有限公司 | Soya seeds extract, manufacture method and application thereof |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2005203059B2 (en) * | 2004-07-16 | 2011-11-24 | Johnson & Johnson Consumer Companies, Inc. | Use of legume products for the treatment and prevention of radiotherapy-induced skin damage |
| CN102920818B (en) * | 2012-11-03 | 2014-06-11 | 牛德寿 | Formula of medicine for treating burn, scald and cold injury |
| TWI640318B (en) * | 2016-06-06 | 2018-11-11 | 儕陞生化技術股份有限公司 | Soybean seed extract, method for producing the same and uses thereof |
| US10543243B2 (en) | 2016-06-06 | 2020-01-28 | Charsire Biotechnology Corp. | Soybeam seed extract, method for producing the same and uses thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1129075A (en) * | 1995-02-16 | 1996-08-21 | 张明伟 | Stomach-care food |
| CN1153457A (en) * | 1994-06-29 | 1997-07-02 | 明治制果株式会社 | Stabilized composition comprising colistin sulfate |
| CN1244351A (en) * | 1998-08-07 | 2000-02-16 | 张铁柱 | Technological process of multielement health food |
-
2003
- 2003-10-20 CN CNB2003101005964A patent/CN100411638C/en not_active Expired - Lifetime
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1153457A (en) * | 1994-06-29 | 1997-07-02 | 明治制果株式会社 | Stabilized composition comprising colistin sulfate |
| CN1129075A (en) * | 1995-02-16 | 1996-08-21 | 张明伟 | Stomach-care food |
| CN1244351A (en) * | 1998-08-07 | 2000-02-16 | 张铁柱 | Technological process of multielement health food |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107456477A (en) * | 2016-06-06 | 2017-12-12 | 侪升生化技术股份有限公司 | Soya seeds extract, manufacture method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1608632A (en) | 2005-04-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP3713537A1 (en) | Topical compositions, process of manufacture and method of use | |
| WO1999038479A1 (en) | Banana peel extract composition and method for extraction | |
| JP4681218B2 (en) | Volatile fractions and compositions from soybean seeds | |
| KR102002894B1 (en) | Cosmetic composition containing the complex natural extracts | |
| KR101252023B1 (en) | Vapor fraction from seeds of glycine max(l.) merr. and composition thereof | |
| Muhammed et al. | Effect of application of Fenugreek (Trigonella foenum-graecum) on skin wound healing in rabbits | |
| CN100411638C (en) | Steam distillate from soybean and its composition | |
| US7112344B2 (en) | Vapor fraction from seeds of Glycine max (L.)Merr. and composition thereof | |
| EP4153321A1 (en) | Topical compositions, process of large-scale manufacture, and method of use | |
| CN108938511B (en) | Composition of natural plant extracts and application of composition in skin moisturizing and acne removing products | |
| KR102051417B1 (en) | The cosmetic composition for the prevention and improvement of striae distensae | |
| CN115518127A (en) | Antibacterial, anti-inflammatory, detumescence and itching relieving cream and preparation method thereof | |
| US7282226B2 (en) | Vapor fraction from seeds of Glycine max (L.) Merr. and composition thereof | |
| EP1506782B1 (en) | Vapor fraction from seeds of glycine max (L.) merr. and composition thereof | |
| CN100350934C (en) | Baidianxiao treaditional Chinese medicine for treating vitiligo and preparation method thereof | |
| KR100381441B1 (en) | The percutaneous form using moxa and moxa mixed with medical plant, making method and using method thereof | |
| CN110624031A (en) | Essential oil for lowering essence, nourishing meridian and health care | |
| CN104547758A (en) | Medicine for treating qi-deficiency and blood-stasis type keloid and preparation method thereof | |
| AU2003231713B2 (en) | Vapor fraction from seeds of glycine max (L.) Merr. and composition thereof | |
| RU2128990C1 (en) | Anti-inflammatory and wound-healing ointment "vundehil" | |
| CN115671239B (en) | Anti-inflammatory, detumescence, antibacterial, antipruritic, anticracking and repairing jelly-curing cream and preparation method thereof | |
| TW200534862A (en) | Vapor fraction from seeds of glycine max (L.) merr. And composition thereof | |
| CN115671034A (en) | Antibacterial, anti-inflammatory, itching-relieving, refreshing and comfortable cream for mosquito bites and preparation method thereof | |
| KR20230022788A (en) | Cosmetic Composition for Cellulite Reduction, Relieving Swell and Skin Irritation Relief Comprising Plant Complex Extracts As Active Ingredients | |
| CN118304335A (en) | Method for extracting purslane extract, method for preparing extract carbomer gel and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20080820 |