IT201800003904A1 - COSMETIC COMPOSITION BASED ON CARBON ANHYDRASE - Google Patents
COSMETIC COMPOSITION BASED ON CARBON ANHYDRASE Download PDFInfo
- Publication number
- IT201800003904A1 IT201800003904A1 IT102018000003904A IT201800003904A IT201800003904A1 IT 201800003904 A1 IT201800003904 A1 IT 201800003904A1 IT 102018000003904 A IT102018000003904 A IT 102018000003904A IT 201800003904 A IT201800003904 A IT 201800003904A IT 201800003904 A1 IT201800003904 A1 IT 201800003904A1
- Authority
- IT
- Italy
- Prior art keywords
- cosmetic composition
- carbonic anhydrase
- skin
- enzyme
- weight
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title claims description 48
- 239000002537 cosmetic Substances 0.000 title claims description 39
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title description 2
- 229910052799 carbon Inorganic materials 0.000 title description 2
- 102000003846 Carbonic anhydrases Human genes 0.000 claims description 47
- 108090000209 Carbonic anhydrases Proteins 0.000 claims description 47
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 44
- 239000001569 carbon dioxide Substances 0.000 claims description 22
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 22
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 claims description 21
- 239000000419 plant extract Substances 0.000 claims description 12
- 239000000284 extract Substances 0.000 claims description 11
- 102000004190 Enzymes Human genes 0.000 claims description 10
- 108090000790 Enzymes Proteins 0.000 claims description 10
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 10
- 239000004480 active ingredient Substances 0.000 claims description 9
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 7
- 102000004317 Lyases Human genes 0.000 claims description 6
- 108090000856 Lyases Proteins 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 239000013598 vector Substances 0.000 claims description 6
- 230000035515 penetration Effects 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000011065 in-situ storage Methods 0.000 claims description 4
- 239000002077 nanosphere Substances 0.000 claims description 4
- 235000013311 vegetables Nutrition 0.000 claims description 4
- 229920000858 Cyclodextrin Polymers 0.000 claims description 3
- 229940097362 cyclodextrins Drugs 0.000 claims description 3
- 239000002502 liposome Substances 0.000 claims description 3
- 239000003094 microcapsule Substances 0.000 claims description 3
- 239000003755 preservative agent Substances 0.000 claims description 3
- 239000003623 enhancer Substances 0.000 claims description 2
- 230000002335 preservative effect Effects 0.000 claims 1
- 210000003491 skin Anatomy 0.000 description 36
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 210000000434 stratum corneum Anatomy 0.000 description 6
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 5
- 210000002615 epidermis Anatomy 0.000 description 5
- 229920002674 hyaluronan Polymers 0.000 description 5
- 229960003160 hyaluronic acid Drugs 0.000 description 5
- 238000011282 treatment Methods 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 210000003780 hair follicle Anatomy 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000002105 nanoparticle Substances 0.000 description 3
- 241000219195 Arabidopsis thaliana Species 0.000 description 2
- 208000035484 Cellulite Diseases 0.000 description 2
- 241000195597 Chlamydomonas reinhardtii Species 0.000 description 2
- 241000195633 Dunaliella salina Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 241001300629 Nannochloropsis oceanica Species 0.000 description 2
- 206010049752 Peau d'orange Diseases 0.000 description 2
- 206010040925 Skin striae Diseases 0.000 description 2
- 206010040954 Skin wrinkling Diseases 0.000 description 2
- 244000300264 Spinacia oleracea Species 0.000 description 2
- 235000009337 Spinacia oleracea Nutrition 0.000 description 2
- 208000031439 Striae Distensae Diseases 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 230000036232 cellulite Effects 0.000 description 2
- 239000006071 cream Substances 0.000 description 2
- 239000002088 nanocapsule Substances 0.000 description 2
- 230000037384 skin absorption Effects 0.000 description 2
- 231100000274 skin absorption Toxicity 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000007920 subcutaneous administration Methods 0.000 description 2
- 230000037303 wrinkles Effects 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 240000006053 Garcinia mangostana Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 239000001166 ammonium sulphate Substances 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 231100000223 dermal penetration Toxicity 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000001723 extracellular space Anatomy 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000010952 in-situ formation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 210000003093 intracellular space Anatomy 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 150000002634 lipophilic molecules Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003961 penetration enhancing agent Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 230000037067 skin hydration Effects 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/14—Liposomes; Vesicles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/738—Cyclodextrins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/06—Preparations for care of the skin for countering cellulitis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/41—Particular ingredients further characterized by their size
- A61K2800/412—Microsized, i.e. having sizes between 0.1 and 100 microns
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/41—Particular ingredients further characterized by their size
- A61K2800/413—Nanosized, i.e. having sizes below 100 nm
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/56—Compounds, absorbed onto or entrapped into a solid carrier, e.g. encapsulated perfumes, inclusion compounds, sustained release forms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Cosmetics (AREA)
Description
DESCRIZIONE DESCRIPTION
del brevetto per invenzione industriale dal titolo: of the patent for industrial invention entitled:
“COMPOSIZIONE COSMETICA A BASE DI ANIDRASI CARBONICA” "COSMETIC COMPOSITION BASED ON CARBON ANHYDRASE"
La presente invenzione è relativa ad una composizione cosmetica a base di anidrasi carbonica. The present invention relates to a cosmetic composition based on carbonic anhydrase.
In particolare, la presente invenzione è relativa ad una composizione ad uso cosmetico per applicazione cutanea a base di anidrasi carbonica. In particular, the present invention relates to a composition for cosmetic use for skin application based on carbonic anhydrase.
Più in particolare, la presente invenzione è relativa ad una preparazione ad uso cosmetico cutaneo a base di anidrasi carbonica per il trattamento di inestetismi quali occhiaie, rughe, cellulite e smagliature. More particularly, the present invention relates to a preparation for skin cosmetic use based on carbonic anhydrase for the treatment of imperfections such as dark circles, wrinkles, cellulite and stretch marks.
Allo stato attuale, per contrastare gli inestetismi cutanei quali occhiaie, rughe, cellulite e smagliature s’interviene con diversi tipi di trattamenti, tra i quali quelli a base di anidride carbonica. La pratica più comunemente utilizzata è la carbossiterapia. At present, different types of treatments are used to combat skin imperfections such as dark circles, wrinkles, cellulite and stretch marks, including those based on carbon dioxide. The most commonly used practice is carboxytherapy.
La carbossiterapia è un trattamento medico estetico che consiste nella somministrazione sottocutanea di anidride carbonica iniettando mediante aghi una piccola quantità di anidride carbonica nella zona interessata. I trattamenti di carbossiterapia devono essere eseguiti da medici specialisti presso strutture accreditate e certificate. La tecnica sopradescritta ha diversi limiti: il fatto che la soluzione al problema sia solo temporanea, quindi per avere effetti duraturi richiede di ripetere le applicazioni nel tempo; sostanzioso dispendio di tempo e denaro; non ultimo la natura invasiva dell’intervento di applicazione che risulta essere piuttosto doloroso. Carboxytherapy is an aesthetic medical treatment that consists in the subcutaneous administration of carbon dioxide by injecting a small amount of carbon dioxide into the affected area using needles. Carboxytherapy treatments must be performed by specialist doctors at accredited and certified facilities. The technique described above has several limitations: the fact that the solution to the problem is only temporary, therefore to have lasting effects it requires repeating the applications over time; substantial expenditure of time and money; not least the invasive nature of the application procedure which turns out to be quite painful.
L’anidrasi carbonica (CA) o carbonato deidratasi è un metallo enzima contenente zinco, ampiamente diffuso in natura sia nei vegetali che negli animali. Tale enzima è naturalmente presente nell’uomo e regola la concentrazione di CO2. L’Anidrasi carbonica catalizza efficacemente la reazione di conversione di anidride carbonica (CO2) in bicarbonato ed acido carbonico (H2CO3) e la reazione inversa, da acido carbonico o bicarbonato in anidride carbonica. La direzione della reazione è regolata dalla concentrazione di CO2: Carbonic anhydrase (CA) or carbonate dehydratase is a metal enzyme containing zinc, widely found in nature in both plants and animals. This enzyme is naturally present in humans and regulates the concentration of CO2. Carbonic anhydrase effectively catalyzes the conversion reaction of carbon dioxide (CO2) into bicarbonate and carbonic acid (H2CO3) and the reverse reaction, from carbonic acid or bicarbonate into carbon dioxide. The direction of the reaction is regulated by the CO2 concentration:
- a concentrazione bassa di CO2 il bicarbonato viene convertito in anidride carbonica; - at a low concentration of CO2 the bicarbonate is converted into carbon dioxide;
- a concentrazione alta di CO2, l’anidride carbonica viene convertita in acido carbonico e bicarbonato. - at a high concentration of CO2, carbon dioxide is converted into carbonic acid and bicarbonate.
Scopo della presente invenzione è fornire una composizione ad uso cosmetico cutaneo che risulti sicura, a basso costo, semplice da applicare ed efficace nella riduzione degli inestetismi, avente, quindi, caratteristiche tali da superare i limiti delle preparazioni ad uso cosmetico note. The object of the present invention is to provide a composition for skin cosmetic use which is safe, low cost, simple to apply and effective in reducing imperfections, thus having characteristics such as to overcome the limits of known cosmetic preparations.
Ulteriore scopo della presente invenzione è fornire una composizione cosmetica a base di anidrasi carbonica che sia applicabile su aree cutanee ampie. A further object of the present invention is to provide a cosmetic composition based on carbonic anhydrase which is applicable on large skin areas.
Secondo la presente invenzione viene realizzata una composizione cosmetica a base di anidrasi carbonica, come definito nella rivendicazione 1. According to the present invention, a cosmetic composition based on carbonic anhydrase is realized, as defined in claim 1.
Per una migliore comprensione della presente invenzione viene ora descritta una forma di realizzazione preferita, a puro titolo di esempio non limitativo, con riferimento ai disegni allegati, nei quali: For a better understanding of the present invention, a preferred embodiment is now described, purely by way of non-limiting example, with reference to the attached drawings, in which:
- la figura 1 mostra uno schema di reazione con produzione di CO2 in situ nell’uso di una composizione cosmetica a base di anidrasi carbonica, secondo l’invenzione; - Figure 1 shows a reaction scheme with in situ CO2 production in the use of a cosmetic composition based on carbonic anhydrase, according to the invention;
- la figura 2 mostra una vista schematica del meccanismo di assorbimento cutaneo di una composizione cosmetica a base di anidrasi carbonica, secondo l’invenzione; - Figure 2 shows a schematic view of the skin absorption mechanism of a cosmetic composition based on carbonic anhydrase, according to the invention;
Con riferimento a tali figure è mostrata una composizione 100 a base di anidrasi carbonica ad uso cosmetico cutaneo, secondo l’invenzione. With reference to these figures, a composition 100 based on carbonic anhydrase for skin cosmetic use is shown, according to the invention.
La composizione 100 a base di anidrasi carbonica ad uso cosmetico cutaneo, comprende un enzima appartenente alla classe delle liasi, almeno un eccipiente comunemente usato per le applicazioni cutanee, almeno un vettore del principio attivo, ossia della liasi. In particolare, l’enzima appartenente alla classe delle liasi è anidrasi carbonica. The composition 100 based on carbonic anhydrase for skin cosmetic use, comprises an enzyme belonging to the class of lyases, at least one excipient commonly used for skin applications, at least one carrier of the active principle, ie lyase. In particular, the enzyme belonging to the lyase class is carbonic anhydrase.
Secondo un aspetto dell’invenzione, la composizione 100 a base di Anidrasi Carbonica ad uso cosmetico cutaneo contiene una percentuale in peso di Anidrasi Carbonica compresa tra 0,1% e 2%. According to one aspect of the invention, the composition 100 based on Carbonic Anhydrase for skin cosmetic use contains a percentage by weight of Carbonic Anhydrase between 0.1% and 2%.
Secondo un aspetto dell’invenzione, i vettori del principio attivo presenti nella composizione cosmetica 100 sono scelti tra i seguenti elementi: Liposomi; Ciclodestrine; nanosfere o microcapsule, esaltatori di penetrazione cutanea. According to one aspect of the invention, the carriers of the active ingredient present in the cosmetic composition 100 are selected from the following elements: Liposomes; Cyclodextrins; nanospheres or microcapsules, skin penetration enhancers.
Secondo un aspetto dell’invenzione, la composizione 100 a base di Anidrasi Carbonica ad uso cosmetico cutaneo contiene anidrasi carbonica estratta da vegetali. According to one aspect of the invention, the composition 100 based on Carbonic Anhydrase for skin cosmetic use contains carbonic anhydrase extracted from vegetables.
Esempi di vegetali i cui estratti hanno concentrazione di anidrasi carbonica idonea ad essere utilizzata nella composizione 100 sono: Examples of plants whose extracts have a concentration of carbonic anhydrase suitable for use in composition 100 are:
- spinacia oleracea, l’estratto di foglie contiene 1-2% delle proteine totali solubili. (L’estratto crudo contiene 10.5 mg/ml di proteine totali di cui 97 U/ml di CA con attività di 73; estratto ottenuto con 30-35% di solfato di ammonio contiene 12.7 mg/ml di proteine totali di cui 1400 U/ml di CA con attività di 1100); - spinacia oleracea, the leaf extract contains 1-2% of the total soluble proteins. (The raw extract contains 10.5 mg / ml of total proteins of which 97 U / ml of CA with activity of 73; extract obtained with 30-35% of ammonium sulphate contains 12.7 mg / ml of total proteins of which 1400 U / ml of CA with activity of 1100);
- pisium Sativum; - pisium Sativum;
- Arabidopsis Thaliana, con ßCA2, ßCA4, CAs che possono arrivare al 1% delle proteine totali nelle foglie; - Arabidopsis Thaliana, with ßCA2, ßCA4, CAs which can reach 1% of the total proteins in the leaves;
- Chlamydomonas Reinhardtii - Chlamydomonas Reinhardtii
- Dunaliella Salina - Dunaliella Salina
- Nannochloropsis Oceanica, anidrasi carbonica tipo Alfa. - Nannochloropsis Oceanica, alpha-type carbonic anhydrase.
Secondo un aspetto dell’invenzione, la composizione 100 a base di Anidrasi Carbonica ad uso cosmetico cutaneo contiene anidrasi carbonica estratta da vegetali scelti nel gruppo costituito da: According to one aspect of the invention, the composition 100 based on Carbonic Anhydrase for skin cosmetic use contains carbonic anhydrase extracted from vegetables selected from the group consisting of:
- spinacia oleracea; - spinacia oleracea;
- pisium Sativum; - pisium Sativum;
- Arabidopsis Thaliana; - Arabidopsis Thaliana;
- Chlamydomonas Reinhardtii; - Chlamydomonas Reinhardtii;
- Dunaliella Salina; - Dunaliella Salina;
- Nannochloropsis Oceanica, anidrasi carbonica tipo Alfa. - Nannochloropsis Oceanica, alpha-type carbonic anhydrase.
Secondo un aspetto dell’invenzione, la composizione cosmetica 100 comprende nanosfere contenenti tra 1% e 20% in peso di estratto vegetale contenente CAs, in cui le percentuali sono riferite all’estratto vegetale. According to one aspect of the invention, the cosmetic composition 100 comprises nanospheres containing between 1% and 20% by weight of plant extract containing CAs, in which the percentages refer to the plant extract.
Secondo un aspetto dell’invenzione la composizione cosmetica 100 comprende tra i suoi elementi costitutivi lo ione idrogeno carbonato (bicarbonato). According to one aspect of the invention, the cosmetic composition 100 includes among its constituent elements the hydrogen carbonate ion (bicarbonate).
Secondo un aspetto dell’invenzione, la composizione 100 è costituita da un enzima appartenente alla classe delle liasi, in particolare l’anidrasi carbonica, bicarbonato o ione idrogeno carbonato, almeno un eccipiente per applicazioni cutanee, ed almeno un vettore del principio attivo. According to one aspect of the invention, composition 100 consists of an enzyme belonging to the class of lyases, in particular carbonic anhydrase, bicarbonate or hydrogen carbonate ion, at least one excipient for skin applications, and at least one carrier of the active ingredient.
Secondo un aspetto dell’invenzione lo ione idrogeno carbonato (bicarbonato) è presente nella composizione cosmetica 100 per un valore compreso nell’intervallo che va dal 1% al 20% del peso totale della preparazione. According to one aspect of the invention, the hydrogen carbonate ion (bicarbonate) is present in the cosmetic composition 100 for a value ranging from 1% to 20% of the total weight of the preparation.
Vantaggiosamente secondo l’invenzione la presenza di ione idrogeno carbonato o bicarbonato e dell’anidrasi carbonica nella composizione 100, consente la produzione di anidride carbonica (CO2) secondo la reazione mostrata in figura 1, con velocità molto alte e direttamente a contatto con la cute, favorendo in tal modo l’assorbimento cutaneo della CO2 e l’effetto cutaneo ridensificante e tonificante. Advantageously, according to the invention, the presence of hydrogen carbonate or bicarbonate ion and carbonic anhydrase in the composition 100 allows the production of carbon dioxide (CO2) according to the reaction shown in figure 1, with very high speeds and directly in contact with the skin. , thus favoring the skin absorption of CO2 and the skin redensifying and toning effect.
Secondo un aspetto dell’invenzione, lo ione idrogeno carbonato o bicarbonato, non è compreso direttamente nella composizione 100 ma viene miscelato alla composizione 100 stessa direttamente sulla cute di un utilizzatore. According to one aspect of the invention, the hydrogen carbonate or bicarbonate ion is not included directly in the composition 100 but is mixed with the composition 100 itself directly on the skin of a user.
Vantaggiosamente secondo l’invenzione, la composizione 100 comprende lo ione idrogeno carbonato (bicarbonato) in un valore compreso nell’intervallo che va dal 1% al 20% o come applicativo da addizionare durante l’applicazione risultando disponibile e efficace nell’innescare l’efficiente reazione di formazione di anidride carbonica direttamente sull’epidermide (strato esterno) o in fase di permeazione, per una azione locale trans-cutanea simile alla carbossiterapia sottocutanea. Advantageously according to the invention, the composition 100 comprises the hydrogen carbonate ion (bicarbonate) in a value ranging from 1% to 20% or as an application to be added during the application resulting available and effective in triggering the efficient reaction of carbon dioxide formation directly on the epidermis (outer layer) or in the permeation phase, for a local trans-cutaneous action similar to subcutaneous carboxytherapy.
Vantaggiosamente secondo l’invenzione, quindi l’anidride carbonica viene prodotta direttamente sulla pelle o in fase di adsorbimento del prodotto per reazione tra l’enzima e il bicarbonato. Advantageously according to the invention, therefore the carbon dioxide is produced directly on the skin or in the adsorption phase of the product by reaction between the enzyme and the bicarbonate.
Secondo un aspetto dell’invenzione la composizione 100 comprende un applicativo contenente acqua, glicerina, bicarbonato di sodio, eccipienti comunemente usati per le applicazioni cutanee. Il bicarbonato può essere incapsulato in matrici polimeriche organiche oppure associato ad un esaltatore di permeazione cutanea, preferibilmente, dimetilsolfossido (DMSO). According to one aspect of the invention, the composition 100 comprises an application containing water, glycerin, sodium bicarbonate, excipients commonly used for skin applications. The bicarbonate can be encapsulated in organic polymeric matrices or associated with a skin permeation enhancer, preferably dimethyl sulfoxide (DMSO).
Secondo un aspetto dell’invenzione, la composizione 100 comprende acqua e un misto di eccipienti comunemente usati in applicazioni cutanee per completare la percentuale del 100%. According to one aspect of the invention, the composition 100 comprises water and a mixture of excipients commonly used in skin applications to complete the percentage of 100%.
Secondo un aspetto dell’invenzione, il procedimento per preparare la composizione cosmetica 100 a base di anidrasi carbonica comprende le fasi di: According to one aspect of the invention, the process for preparing the cosmetic composition 100 based on carbonic anhydrase includes the steps of:
- preparare l'enzima Anidrasi Carbonica proveniente da estratti vegetali; - prepare the carbonic anhydrase enzyme from plant extracts;
- preparare almeno un eccipiente comunemente usato per le applicazioni cutanee; - prepare at least one excipient commonly used for skin applications;
- preparare almeno un vettore dell'enzima Anidrasi Carbonica; e - preparing at least one carrier of the carbonic anhydrase enzyme; And
- incapsulare in un vettore una quantità compresa tra 1% e 20% in peso di un estratto vegetale contenente anidrasi carbonica; - encapsulating in a vector an amount comprised between 1% and 20% by weight of a vegetable extract containing carbonic anhydrase;
- addizionare una quantità compresa tra 0,1-1% in peso di un agente conservante; - adding an amount comprised between 0.1-1% by weight of a preservative agent;
- completare le percentuali mancanti al 100% in peso con acqua e un misto di eccipienti comunemente usati in applicazioni cutanee. - complete the missing percentages to 100% by weight with water and a mixture of excipients commonly used in skin applications.
Secondo un aspetto dell’invenzione, un procedimento per la produzione in situ di anidride carbonica, a livello cutaneo, comprende una reazione tra enzima anidrasi carbonica e bicarbonato contenuti in una composizione cosmetica 100. According to one aspect of the invention, a process for the in situ production of carbon dioxide, at the skin level, comprises a reaction between the enzyme carbonic anhydrase and bicarbonate contained in a cosmetic composition 100.
Il Richiedente, a dimostrazione dell’efficacia dell’invenzione, ha effettuato sperimentazioni e studi su casi affini di preparazione e veicolazione di attivi in applicazioni cutanee. The Applicant, to demonstrate the effectiveness of the invention, has carried out experiments and studies on similar cases of preparation and delivery of active ingredients in skin applications.
La veicolazione di estratti vegetali ricchi in attivi incrementa la biodisponibilità, facilità la permeazione cutanea e stabilizza l’attivo nella formulazione. È dimostrato che l’incapsulamento in vettori quali Liposomi, Ciclodestrine, Nanosfere o microcapsule ne favorisce la penetrazione attraverso lo strato corneo verso gli strati più profondi della pelle dove l'estratto e i principi attivi verranno rilasciati. The conveyance of plant extracts rich in active ingredients increases bioavailability, facilitates skin permeation and stabilizes the active in the formulation. It has been shown that encapsulation in vectors such as Liposomes, Cyclodextrins, Nanospheres or microcapsules favors their penetration through the stratum corneum towards the deeper layers of the skin where the extract and active ingredients will be released.
I vettori garantiscono un sistema di trasporto ideale dei principi attivi in applicazione cutanee, consentendone il rilascio controllato e mirato, l'isolamento e una maggiore stabilità ed efficacia. The carriers guarantee an ideal transport system of the active ingredients in skin application, allowing their controlled and targeted release, isolation and greater stability and effectiveness.
A causa delle loro dimensioni, le nanocapsule contenenti principi attivi possono penetrare negli spazi intracellulari o intercellulari e attraverso i follicoli piliferi; questo li rende un trasportatore cutaneo ideale per estratti vegetali in applicazioni cutanee. Negli ultimi anni, evidenze sperimentali supportano la fattibilità dell'utilizzo di nanoparticelle o nanocapsule per il rilascio di estratti vegetali nella pelle. Due to their size, the nanocapsules containing active ingredients can penetrate into the intracellular or intercellular spaces and through the hair follicles; this makes them an ideal skin transporter for plant extracts in skin applications. In recent years, experimental evidence supports the feasibility of using nanoparticles or nanocapsules for the release of plant extracts into the skin.
Estratti vegetali liberi e incapsulati sono stati incorporati in creme o sistemi acquosi, ed è stato determinato che entrambe le formulazioni in crema (estratto libero e incapsulato) penetravano più in profondità nei follicoli piliferi rispetto a quelle applicate nei sistemi acquosi. A titolo di esempio, l'estratto di G. mangostana incorporato nelle nanoparticelle ha mostrato una distribuzione più alta e omogenea nei livelli dello strato corneo rispetto all'estratto libero. Risultati sperimentali mostrano che la vitalità e la penetrazione dermica dell'estratto vegetale aumentano con la presenza di vettori. Free and encapsulated plant extracts were incorporated into creams or aqueous systems, and it was determined that both cream formulations (free and encapsulated extract) penetrated deeper into hair follicles than those applied in aqueous systems. As an example, G. mangostana extract incorporated in the nanoparticles showed a higher and more homogeneous distribution in the stratum corneum levels than the free extract. Experimental results show that the vitality and dermal penetration of the plant extract increase with the presence of vectors.
Formulazioni contenenti nanoparticelle ed estratti vegetali incapsulati, principalmente, mantengono e aumentano l'attività biologica degli estratti incorporati. Formulations containing nanoparticles and encapsulated plant extracts, mainly, maintain and increase the biological activity of the incorporated extracts.
Il trasporto attraverso lo strato corneo è limitato a molecole a bassa massa molecolare e a moderata lipofilicità, con sufficiente solubilità nel dominio liquido dello strato corneo pur mantenendo sufficiente idrofilicità per consentire la partizione nell'epidermide. Micro/nano vettori contenenti attivi penetrano nei canali dello strato corneo e dei follicoli piliferi, rilasciando gli ingredienti incapsulati in situ. Transport through the stratum corneum is limited to low molecular mass and moderate lipophilic molecules, with sufficient solubility in the liquid domain of the stratum corneum while maintaining sufficient hydrophilicity to allow partitioning into the epidermis. Micro / nano vectors containing active penetrate into the canals of the stratum corneum and hair follicles, releasing the encapsulated ingredients in situ.
Con riferimento all’enzima Anidrasi Carbonica e alla sua penetrazione cutanea attraverso preparati cosmetici, si portano ad esempio recenti studi di permeazione cutanea di acido ialuronico (HA) con diversi pesi molecolari che permettono di prevedere il comportamento dell’enzima Anidrasi Carbonica in fase di adsorbimento cutaneo. Questi risultati mostrano che la permeazione dell'HA aumenta con la diminuzione del peso molecolare dell'HA stesso. Questo è in accordo con altri studi che dimostrano che l'HA a basso peso molecolare (50 kDa) è associato a un significativo miglioramento dell'idratazione della pelle dovuto ad una penetrazione negli strati epidermici più profondi. In analogia a questi risultati si prevede che l’enzima opportunamente veicolato penetri nello strato profondo dell’epidermide grazie al basso peso molecolare (29-30 kDa). With reference to the enzyme Carbonic Anhydrase and its cutaneous penetration through cosmetic preparations, recent studies of skin permeation of hyaluronic acid (HA) with different molecular weights that allow to predict the behavior of the enzyme Carbonic Anhydrase in the adsorption phase are mentioned. cutaneous. These results show that the permeation of HA increases as the molecular weight of the HA decreases. This is in agreement with other studies showing that low molecular weight (50 kDa) HA is associated with a significant improvement in skin hydration due to penetration into the deeper epidermal layers. Similarly to these results, it is expected that the appropriately conveyed enzyme penetrates the deep layer of the epidermis thanks to its low molecular weight (29-30 kDa).
Il Richiedente ha quindi dimostrato, alla luce di quanto evidenziato in merito al trasporto e alla permeazione di attivi in preparazioni cosmetiche per uso cutaneo, l’efficacia della composizione cosmetica a uso cutaneo secondo l’invenzione e l’efficiente reazione di conversione dell’idrogeno carbonato in anidride carbonica catalizzata dall’Anidrasi Carbonica che può avvenire in fase superficiale a contatto con l’epidermide e in fase di diffusione oltre lo strato corneo per un trattamento di carbossi-terapia trans-cutaneo per una formazione in situ di anidride carbonica dipendente dalla presenza dell’enzima Anidrasi Carbonica e idrogeno carbonato. The Applicant has therefore demonstrated, in the light of what has been highlighted regarding the transport and permeation of active ingredients in cosmetic preparations for skin use, the effectiveness of the cosmetic composition for skin use according to the invention and the efficient hydrogen conversion reaction. carbonate in carbon dioxide catalyzed by carbonic anhydrase which can occur in the superficial phase in contact with the epidermis and in the diffusion phase beyond the stratum corneum for a trans-cutaneous carboxy-therapy treatment for an in situ formation of carbon dioxide dependent on the presence of the enzyme Carbonic Anhydrase and hydrogen carbonate.
Vantaggiosamente secondo l’invenzione, la composizione cosmetica a base di anidrasi carbonica è facilmente assorbibile dalla cute, e risulta efficace contro gli inestetismi cutanei. Advantageously according to the invention, the cosmetic composition based on carbonic anhydrase is easily absorbable by the skin, and is effective against skin imperfections.
Inoltre, l’uso della composizione cosmetica a base di anidrasi carbonica è vantaggiosamente semplice e indolore e non presenta controindicazioni mediche. In addition, the use of the cosmetic composition based on carbonic anhydrase is advantageously simple and painless and has no medical contraindications.
Risulta, infine, chiaro che alla composizione a base di anidrasi carbonica ad uso cosmetico cutaneo qui descritto ed illustrato possono essere apportate modifiche e varianti senza per questo uscire dall’ambito protettivo della presente invenzione, come definito nelle rivendicazioni allegate. Finally, it is clear that modifications and variations may be made to the composition based on carbonic anhydrase for cosmetic skin use described and illustrated here without thereby departing from the protective scope of the present invention, as defined in the attached claims.
Claims (10)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT102018000003904A IT201800003904A1 (en) | 2018-03-23 | 2018-03-23 | COSMETIC COMPOSITION BASED ON CARBON ANHYDRASE |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT102018000003904A IT201800003904A1 (en) | 2018-03-23 | 2018-03-23 | COSMETIC COMPOSITION BASED ON CARBON ANHYDRASE |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IT201800003904A1 true IT201800003904A1 (en) | 2019-09-23 |
Family
ID=62597914
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IT102018000003904A IT201800003904A1 (en) | 2018-03-23 | 2018-03-23 | COSMETIC COMPOSITION BASED ON CARBON ANHYDRASE |
Country Status (1)
| Country | Link |
|---|---|
| IT (1) | IT201800003904A1 (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995030426A1 (en) * | 1994-05-09 | 1995-11-16 | Serge Camprasse | Cutaneous rejuvenating and healing product, method for its manufacture and uses thereof |
| US5773034A (en) * | 1994-05-09 | 1998-06-30 | Fortune Base Management, Ltd. | Cutaneous rejuvenating and healing product, method for its manufacture and uses thereof |
| US20100247439A1 (en) * | 2009-03-27 | 2010-09-30 | Choon Young Lee | Dendritic nano-antioxidants |
| EP3120830A1 (en) * | 2015-07-21 | 2017-01-25 | Greenaltech, S.L. | Compositions for protecting skin comprising dna repair enzymes and phycobiliprotein |
| US20170128355A1 (en) * | 2015-11-10 | 2017-05-11 | Vincent C. Giampapa | Topical composition for skin treatment to reduce lines and wrinkles of the face and body using a dual dna repair mechanism to address damage caused by aging and ultra-voilet induced damage to the skin with combination of skin turgor enhancement compounds |
-
2018
- 2018-03-23 IT IT102018000003904A patent/IT201800003904A1/en unknown
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995030426A1 (en) * | 1994-05-09 | 1995-11-16 | Serge Camprasse | Cutaneous rejuvenating and healing product, method for its manufacture and uses thereof |
| US5773034A (en) * | 1994-05-09 | 1998-06-30 | Fortune Base Management, Ltd. | Cutaneous rejuvenating and healing product, method for its manufacture and uses thereof |
| US20100247439A1 (en) * | 2009-03-27 | 2010-09-30 | Choon Young Lee | Dendritic nano-antioxidants |
| EP3120830A1 (en) * | 2015-07-21 | 2017-01-25 | Greenaltech, S.L. | Compositions for protecting skin comprising dna repair enzymes and phycobiliprotein |
| US20170128355A1 (en) * | 2015-11-10 | 2017-05-11 | Vincent C. Giampapa | Topical composition for skin treatment to reduce lines and wrinkles of the face and body using a dual dna repair mechanism to address damage caused by aging and ultra-voilet induced damage to the skin with combination of skin turgor enhancement compounds |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2934455B1 (en) | Combination of active agents comprising at least one essential oil, one cyclodextrin and one liquid fatty substance and composition comprising it | |
| US9468597B1 (en) | Stabilized L-ascorbic acid skin serum | |
| FR2962333A1 (en) | Composition, useful for the preventive and/or curative treatment of skin disorders caused by Propionibacterium acnes e.g. acne and rosacea, comprises 2-alkoxy-4-alkylketone phenol compound and essential oil in medium | |
| KR102304048B1 (en) | PLGA nanoparticles for cosmetic comprising natural plant extracts and a method for producing the same | |
| CN105055296A (en) | Skin care product composition containing saccharomycetes fermentation product filter liquor | |
| KR102547129B1 (en) | fucoidan hair restorer | |
| CN106389171A (en) | Purely-botanic natural-component-compounded hydrolat-series toner and making method thereof | |
| US10206869B2 (en) | Obtaining an extract from brown algae gametophytes, and use of said extract as a cosmetic anti-aging active principle | |
| CN112826847A (en) | Microneedle patch for removing acne and removing acne marks and preparation method thereof | |
| EP1616551A1 (en) | Cosmetic treatment for preventing or delaying the signs of skin ageing | |
| KR100984010B1 (en) | Compound for trichogenousness and preventing the depilation | |
| Esentürk-Güzel et al. | Ammi visnaga L. and Nanocarrier Approaches in the Treatment of Skin Diseases | |
| IT201800003904A1 (en) | COSMETIC COMPOSITION BASED ON CARBON ANHYDRASE | |
| CN104784086B (en) | Nti-freckle compound nanometer liposomes, its preparation method and its application | |
| FR3010306A1 (en) | COSMETIC COMPOSITION COMPRISING A LAMELLAR SYSTEM | |
| CN108697639B (en) | Percutaneous absorption composition and application thereof in preparing percutaneous absorption preparation | |
| Vogel et al. | Challenges of nanotechnology in cosmetic permeation with caffeine | |
| US20090123406A1 (en) | Organic therapeutic and cosmetic preparation | |
| KR102205259B1 (en) | Development of antioxidant black vitamin by using vitamin C-chitosan complex and its application into cosmetic ingredients for blue light protection | |
| CN113786394A (en) | Drug delivery system for preventing and treating alopecia, preparation method thereof and microneedle patch | |
| CN106420678A (en) | Phenolic hydroxyl-containing aryl compound acting as tyrosinase activator and application thereof | |
| FR2873026A1 (en) | Use of a transdermal delivery system comprising a composition containing a non-indispensable micronutrient for the treatment-, prevention-, retardation- and/or limitation of signs of ageing of skin, mucous membranes or appendages | |
| JPH10175814A (en) | Skin preparation for external use and bathing agent containing garcinia extract | |
| EA026733B1 (en) | Skin lightening composition | |
| KR20100094109A (en) | Functional skin lotion including date |