WO2010057555A1 - Agitateur pour bioréacteur - Google Patents

Agitateur pour bioréacteur Download PDF

Info

Publication number
WO2010057555A1
WO2010057555A1 PCT/EP2009/007474 EP2009007474W WO2010057555A1 WO 2010057555 A1 WO2010057555 A1 WO 2010057555A1 EP 2009007474 W EP2009007474 W EP 2009007474W WO 2010057555 A1 WO2010057555 A1 WO 2010057555A1
Authority
WO
WIPO (PCT)
Prior art keywords
arm
holder
arms
container
pivotally
Prior art date
Application number
PCT/EP2009/007474
Other languages
German (de)
English (en)
Inventor
Oscar-Werner Reif
Shahmir Fatherazi
Gerhard Greller
Original Assignee
Sartorius Stedim Biotech Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sartorius Stedim Biotech Gmbh filed Critical Sartorius Stedim Biotech Gmbh
Priority to EP09740067.5A priority Critical patent/EP2358466B1/fr
Priority to US13/129,700 priority patent/US8951785B2/en
Publication of WO2010057555A1 publication Critical patent/WO2010057555A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/05—Stirrers
    • B01F27/051—Stirrers characterised by their elements, materials or mechanical properties
    • B01F27/054—Deformable stirrers, e.g. deformed by a centrifugal force applied during operation
    • B01F27/0541—Deformable stirrers, e.g. deformed by a centrifugal force applied during operation with mechanical means to alter the position of the stirring elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/40—Mounting or supporting mixing devices or receptacles; Clamping or holding arrangements therefor
    • B01F35/41—Mounting or supporting stirrer shafts or stirrer units on receptacles
    • B01F35/412—Mounting or supporting stirrer shafts or stirrer units on receptacles by supporting both extremities of the shaft
    • B01F35/4121—Mounting or supporting stirrer shafts or stirrer units on receptacles by supporting both extremities of the shaft at the top and at the bottom of the receptacle, e.g. for performing a conical orbital movement about a vertical axis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/50—Mixing receptacles
    • B01F35/51—Mixing receptacles characterised by their material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/50—Mixing receptacles
    • B01F35/513—Flexible receptacles, e.g. bags supported by rigid containers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements

Definitions

  • the invention relates to a stirrer for bioreactors, which are in particular designed as containers with flexible walls, with a plurality of hingedly interconnected arms, which are rotatable together about an axis of rotation, wherein a first arm with its first arm end rotatable about the axis of rotation on a first holder is arranged, which is attached to a first side of the container.
  • stirrers prevent cell deposition in bioreactors and disperse the air or gases in fumigated bioreactors.
  • a stirrer for a container which consists of several articulated arms which can be inserted in the extended state through a container opening into the container, wherein when touching the container bottom some legs open to the outside.
  • a first arm is rotatable together with the remaining arms connected to it together about a rotation axis. there the first arm is rotatably disposed with its first arm end against a first support fixed to a first vertically upper side of the container.
  • a disadvantage of the known Ruhrer is that he with his lower two arms when rotating on the lower side, the Behalter Guinea grinds. In the case of a metal container, this grinding produces undesirable particles, while in the case of a container made of plastic film, the film would be damaged after a short operating time. A horizontal or oblique operation is not possible with such a dysentery.
  • a hinged baffle system or a foldable steering plate device which can be used in a container or bioreactor through an opening which is smaller than the Behalter barnmesser.
  • the harassment arrangement is composed of several rings and long segments or arms, wherein the long segments are extended after insertion into the container during placement and can create so as to harass the Behalterwanden. The harassment then act as a harassment or stream breaker while stirring with a dysentery.
  • the object of the present invention is therefore to provide a foldable stirrer which can be folded to save space and, in particular, can be inserted into a container with flexible walls.
  • the arrangement of two brackets arranged on opposite end faces of the container reliably prevents unwanted grinding on the lower side of the container in the vertical direction.
  • a stirrer works not only in a vertical but also in an oblique or horizontal position.
  • the stirrer adapts to different heights of a container and is pulled apart when filling a flexible container with increasing container volume and opened.
  • the stirrer works in both partially open and fully opened state.
  • two arms are provided, wherein the first arm is pivotally connected at its second arm end to the first arm end of the second arm forming the last arm.
  • Such a stirrer is simple and compact and particularly suitable for smaller and medium containers.
  • two opposing pairs of arms are provided, wherein the first arm of each arm pair with its first arm end to the first holder and with its second arm end to the first arm end of the second arm, which forms the last arm of the arm pair, pivotally is connected, and wherein the second arm of each pair of arms with its second arm end is pivotally connected to the second bracket.
  • a stirrer is just as flat zusafftenfaltbar as a corresponding stirrer with only one pair of arms, but has a double stirring surface.
  • three arms are provided, wherein the second arm is pivotally connected with its second arm end to the first arm end of the third arm, which forms the last arm and whose second end is in communication with the second bracket.
  • Stirrers with three or more arms are particularly suitable for medium and larger containers.
  • the second arm arranged between the first arm and the third arm as the middle arm has twice the length of the other arms.
  • This stirrer is also for larger containers suitable.
  • it is favorable that the stirrer is arranged symmetrically to the vertical axis of rotation.
  • the first holder has a fixed base part which is fixed to the inner surface of the first side of the container.
  • the first holder has a rotatably mounted in the base part Achsteil, which is pivotally connected at its the container interior first Achs inconveniencede with the first arm end of the first arm and the remote from the container interior second Achsutzde with a drive axle of a drive is connected.
  • the second holder also has a fixed base part which is fixed to the inner surface of the second side of the container, for example the bottom surface.
  • the second holder has an axle part which is rotatably arranged in the base part and is pivotably connected to the second arm end of the last arm at its first axle end facing the container interior and is rotatably mounted in the base part with its second axle end facing away from the container interior.
  • the base parts of the brackets to weld with each adjacent side of the container.
  • the axle parts are mounted in a preferred embodiment by plain bearings in the base parts.
  • At least one of the arms has at least one stirring blade.
  • two arms for example, they each have two stirring blades on their outer sides facing away from each other.
  • the middle arm as far as it is at least twice as long as the other two arms on its opposite sides in each case from end to center have a convexly curved stirring blade. A folding in a plane is still possible.
  • a stirring blade is hinged on the one hand pivotally connected to the hinge connection between the first end of the last arm and the second end of the adjacent arm and on the other hand pivotally hinged to a first end of a guide rod pivotally connected at its second end to the second Support is hinged and keeps the stirring blade at different distances of the brackets in the vertical direction in the same horizontal position. Accordingly, it is possible with two pairs of arms opposite each other, that each pair of arms has a pivotable stirring blade, which is held in each case by a guide rod in a horizontal position.
  • Figure 1 a spatial representation of a foldable
  • Figure 2 a spatial representation of a foldable
  • FIG. 3 a three-dimensional representation of a foldable stirrer with four arms
  • Figure 4 a spatial representation of a foldable
  • Figure 5 a spatial representation of a foldable
  • FIG. 6 a spatial representation of the stirrer of FIG. 5 in a further unfolded position in a reduced representation
  • FIG. 7 shows a spatial representation of the foldable stirrer of FIG. 5 in a completely unfolded position in reduced representation
  • 8 is a perspective view of a holder for Ruhrblatter with an external linkage for a Fuhrungsstange in an enlarged view
  • Figure 9 exne spatial representation of a holder for Ruhrblatter with internal articulation of a Fuhrungsstange in an enlarged view
  • Figure 10 a spatial representation of another
  • Figure 12 is a side view of a foldable Ruhrers with two opposite arm pairs, each with a Ruhrblatt and hinged Fuhrungsstange and
  • Figure 13 a spatial representation of a foldable
  • a stirrer 1 essentially consists of interconnected arms 2, 3, a first holder 4 and a second holder 5.
  • the first holder 4 has a fixed base part 6, in which an axle part 7 is rotatably mounted on.
  • the axle part 7 has a first axle end 8 which is pivotally connected to a first arm end 9 about a transversely to a rotational axis 10 extending transverse axis 11.
  • the axle 7 is connectable to a drive axle 13 of a drive 14.
  • the second holder 5 has a base part 15, in which an axle part 16 is rotatably mounted.
  • the axle member 16 is pivotally connected at its first bracket 4 facing first Achs toode 17 with a second arm end 18 of the second arm 3.
  • the second arm 3 is pivotally connected at its second arm end 18 facing away from the first arm end 19 with a first arm end 9 remote from the second arm end 20 of the first arm 2.
  • the second arm 3 of the exemplary embodiments according to FIGS. 1, 2, 5, 6, 7 and 12 in each case forms the last arm of the arms 2, 3 pivotally connected to one another.
  • the arms 2, 3 can be rotated together with the axle parts 7, 16 about the axis of rotation 10.
  • a bioreactor which is as a container 21 with flexible walls, ie at least one flexible side wall 22, a first vertically upper side 23 and a lower side lying in the vertical direction second side 24 is formed by welding of the base part 6 of the first holder 4 with the first side 23 and the second base part 15 of the second holder 5 with the second side 24 secured in the container.
  • the arms 2, 3 at their opposite outer sides 25, 26 each have two stirring blades 27.
  • FIG. 3 shows a stirrer 1 'having a first arm 2, a second arm 3, a third arm 28 and a fourth arm 29.
  • the fourth arm 29 forms the last arm, which is connected to its second arm end 30 to the first Achs moneyde 17 of the axle 16 of the second bracket 5 pivotally about the transverse axis 11.
  • the stirrer 1 has a first arm 2, a second arm 3" and a third arm 28.
  • the third arm 28 forms the last arm, which is pivotally connected at its second end 49 to the second holder 5.
  • the second arm 3 '' is twice as long as its adjacent arms 2, 28.
  • the second arm 3 '' carries at its two opposite outer sides offset by half its length in each case a convexly shaped stirring blade 31.
  • Der third arm 28 is pivotally connected at its first end 50 to the second end 18 "of the second arm 3".
  • FIG. 5 to 7 shows a stirrer 1 '' ', which has at its articulation between the first arm 2 and second arm 3 a pivotable about a connecting axis 32 blade holder 33 for securing a plurality, in the exemplary embodiment six, stirring blades 34.
  • the blade holder 33 has a second articulation point 35, on which it is articulated pivotably with a first end 36 of a guide rod 37.
  • the guide rod 37 extends approximately parallel to the second arm 3 and is articulated with its first end 36 facing away from the second end 38 to the axle portion 16 '' 'of the second holder 5' ''.
  • FIG. 6 shows the stirrer 1 '' 'in a middle position
  • FIG. 7 shows the stirrer 1' '' in a completely extended or unfolded position.
  • Figure 8 shows a blade holder 39 with an outer pivot point 40 for supporting a stirring blade, not shown.
  • FIG. 9 shows a further blade holder 41 with an inner pivot point 42.
  • FIG. 10 shows a further embodiment of a blade holder 43 with an outer articulation point 44
  • FIG. 11 shows the blade holder 33 with the inner articulation point 35 according to the exemplary embodiment of FIG. 5, but without depiction of the stirring blades.
  • the embodiment of Figure 12 shows a Ruhrer ⁇ '''with two opposing arm pairs 44, 45. Each pair of arms 44, 45 has at its articulation between the first arm 2 and second arm 3, a blade holder 46 with a Ruhrblatt 47.
  • the blade holder 46 is connected via its articulation point 48 with the Fuhrungsstab 37, which is hinged at its second end 38 on the axle portion 16 '''' of the second holder 5 '''' pivotally.
  • FIG. 13 shows a stirrer 51 with three arms (2, 3, 28), which has a second blade holder 52 for a plurality of rotary blades 34, which on the one hand pivotably on the articulated connection between the first arm end 19 of the second arm 3 and the second arm end 20 of the adjacent first arm 2 is articulated.
  • the second blade holder 52 is pivotally hinged to a first end 53 of a second Fuhrungsstabes 54 which is pivotally connected at its second end 55 to the first blade holder 33 and the second blade holder 52 with the Ruhrblattern 34 at different distances of the brackets 4, 5 ' '' in the vertical direction in the same horizontal position hold.

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Mixers Of The Rotary Stirring Type (AREA)

Abstract

La présente invention concerne un agitateur (1, 1', 1", 1"', 1"") pour bioréacteurs, notamment pour ceux conçus sous forme de récipients (21) dotés de parois flexibles (22), ayant une pluralité de bras (2, 3, 28, 29) articulés les uns aux autres et pouvant tourner ensemble autour d'un axe de rotation (10). Un premier bras (2), pouvant tourner avec sa première extrémité (9) autour de l'axe de rotation (10), est agencé sur un premier élément de retenue (4) fixé sur un premier côté (23) du récipient (21). Avec sa seconde extrémité (18, 20, 30) éloignée du premier bras, le dernier bras (3, 28, 29), éloigné du premier bras (2) (2), est agencé sur un second élément de retenue (5, 5''', 5'''') de manière à pouvoir tourner autour de l'axe de rotation (10), le second élément de retenue (5, 5''', 5'''') étant fixé sur un second côté (24) du récipient (21) situé à l'opposé du premier côté (23) du récipient (21), les extrémités des bras (9, 18, 19, 20, 30, 49) reliées aux éléments de retenue (4, 5, 5''', 5'''') ou aux extrémités voisines (19, 20) pouvant pivoter respectivement autour d'un axe transversal à l'axe de rotation (10).
PCT/EP2009/007474 2008-11-20 2009-10-19 Agitateur pour bioréacteur WO2010057555A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP09740067.5A EP2358466B1 (fr) 2008-11-20 2009-10-19 Agitateur pour bioréacteur
US13/129,700 US8951785B2 (en) 2008-11-20 2009-10-19 Stirrer for a bioreactor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102008058338A DE102008058338B4 (de) 2008-11-20 2008-11-20 Rührer für Bioreaktor
DE102008058338.3 2008-11-20

Publications (1)

Publication Number Publication Date
WO2010057555A1 true WO2010057555A1 (fr) 2010-05-27

Family

ID=41510844

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/007474 WO2010057555A1 (fr) 2008-11-20 2009-10-19 Agitateur pour bioréacteur

Country Status (4)

Country Link
US (1) US8951785B2 (fr)
EP (1) EP2358466B1 (fr)
DE (1) DE102008058338B4 (fr)
WO (1) WO2010057555A1 (fr)

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CN104826527A (zh) * 2015-05-21 2015-08-12 苏州欢颜电气有限公司 一种升降式一体复合型搅拌装置
CN104857872A (zh) * 2015-05-21 2015-08-26 苏州欢颜电气有限公司 一种顶入式可伸缩的组合搅拌装置
CN109012273A (zh) * 2018-08-12 2018-12-18 惠州市无龄康态健康科技有限公司 一种收放伞状搅拌装置

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US9700857B1 (en) 2012-03-23 2017-07-11 Life Technologies Corporation Fluid mixing system with drive shaft steady support
WO2013151733A1 (fr) * 2012-04-06 2013-10-10 Hyclone Laboratories, Inc. Système de mélange de fluide avec ligne d'entraînement flexible et roue à aubes pliable
NL1040495C2 (nl) * 2012-11-14 2014-05-15 Luc Hyfte Menger.
EP3122446B1 (fr) 2014-03-22 2018-07-18 Life Technologies Corporation Ensembles d'impulseur pour systèmes de traitement de fluide
DE102014017189A1 (de) * 2014-11-21 2016-05-25 Infors Ag Reaktor, Reinigungsverfahren für diesen sowie Verwendung
KR101629150B1 (ko) 2015-02-28 2016-06-09 김두현 일회용 컨테이너를 이용하는 바이오리액터용 교반기
CN104826526B (zh) * 2015-05-21 2017-05-24 永春县桃源纸织画院 一种升降式可伸缩的组合搅拌装置
CN104826525B (zh) * 2015-05-21 2017-05-24 永春雪山靓雪矿泉水有限公司 一种升降式可伸缩的分体组合搅拌装置
CN104815577B (zh) * 2015-05-21 2017-05-24 永春县桃源纸织画院 一种升降式分体复合型搅拌装置
CN104826530B (zh) * 2015-05-21 2017-05-24 永春雪山靓雪矿泉水有限公司 一种加强型伸缩式搅拌装置
CN104841317B (zh) * 2015-05-21 2017-06-20 谢立群 一种随液面高度自动调整的搅拌装置
CN104841310B (zh) * 2015-05-21 2017-05-24 永春琦承茶叶专业合作社 一种伸缩式搅拌装置
CN104841309B (zh) * 2015-05-21 2017-05-24 惠安易成机电设备工程有限公司 一种顶入式一体复合型搅拌装置
CN104841314B (zh) * 2015-05-21 2017-05-24 泉州市梅森纸织画艺术研究院有限公司 一种顶入式伸缩搅拌装置
FR3039776B1 (fr) 2015-08-03 2017-08-25 Sartorius Stedim Fmt Sas Procede d'assemblage d'un recipient-melangeur comprenant un arbre telescopique.
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US11788048B2 (en) * 2017-09-19 2023-10-17 Life Technologies Corporation Systems and methods for a collapsible chamber with foldable mixing element
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WO2019143477A1 (fr) 2018-01-17 2019-07-25 Life Technologies Corporation Systèmes de mélange de fluide comprenant un ensemble de mélange hélicoïdal doté d'une fixation d'agitateur et procédés d'utilisation
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WO2020041422A1 (fr) 2018-08-21 2020-02-27 Lifecycle Biotechnologies, Lp Système de bioréacteur oscillant
CN108972886B (zh) * 2018-09-17 2021-06-22 东阳市天杨建筑工程设计有限公司 混凝土搅拌器
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WO2021158555A1 (fr) 2020-02-03 2021-08-12 Life Technologies Corporation Systèmes de mélange de fluides à impulseurs modulaires et procédés associés
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CN113828586A (zh) * 2021-10-21 2021-12-24 江苏长寿集团有限公司 一种粽子生产用糯米智能清洗沥干设备及其操作方法
CN116203139B (zh) * 2023-05-06 2023-07-14 山东泰阳特种设备检测科技有限公司 一种常压储罐内壁焊缝无损检测装置

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US857683A (en) 1906-11-28 1907-06-25 Lionel M Stern Agitator.
US1661255A (en) 1925-03-07 1928-03-06 Fred H Haywood Barrel agitator
US2045710A (en) 1935-04-13 1936-06-30 Fred H Haywood Barrel agitator
US20020105856A1 (en) 2001-02-06 2002-08-08 Alexandre Terentiev Apparatus and method for mixing materials sealed in a container under sterile conditions
US6769800B1 (en) 2002-09-23 2004-08-03 The United States Of America As Represented By The Secretary Of Army Collapsing and telescoping baffles for stirred vessels

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Publication number Priority date Publication date Assignee Title
US857683A (en) 1906-11-28 1907-06-25 Lionel M Stern Agitator.
US1661255A (en) 1925-03-07 1928-03-06 Fred H Haywood Barrel agitator
US2045710A (en) 1935-04-13 1936-06-30 Fred H Haywood Barrel agitator
US20020105856A1 (en) 2001-02-06 2002-08-08 Alexandre Terentiev Apparatus and method for mixing materials sealed in a container under sterile conditions
US6769800B1 (en) 2002-09-23 2004-08-03 The United States Of America As Represented By The Secretary Of Army Collapsing and telescoping baffles for stirred vessels

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104826527A (zh) * 2015-05-21 2015-08-12 苏州欢颜电气有限公司 一种升降式一体复合型搅拌装置
CN104857872A (zh) * 2015-05-21 2015-08-26 苏州欢颜电气有限公司 一种顶入式可伸缩的组合搅拌装置
CN109012273A (zh) * 2018-08-12 2018-12-18 惠州市无龄康态健康科技有限公司 一种收放伞状搅拌装置

Also Published As

Publication number Publication date
EP2358466A1 (fr) 2011-08-24
US20110229963A1 (en) 2011-09-22
DE102008058338A1 (de) 2010-05-27
US8951785B2 (en) 2015-02-10
EP2358466B1 (fr) 2013-08-07
DE102008058338B4 (de) 2010-11-11

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