WO2009129797A3 - Stabile lysepuffermixtur zur extraktion von nukleinsäuren - Google Patents

Stabile lysepuffermixtur zur extraktion von nukleinsäuren Download PDF

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Publication number
WO2009129797A3
WO2009129797A3 PCT/DE2009/000549 DE2009000549W WO2009129797A3 WO 2009129797 A3 WO2009129797 A3 WO 2009129797A3 DE 2009000549 W DE2009000549 W DE 2009000549W WO 2009129797 A3 WO2009129797 A3 WO 2009129797A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acids
lysis buffer
stable
mixture
buffer mixture
Prior art date
Application number
PCT/DE2009/000549
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English (en)
French (fr)
Other versions
WO2009129797A2 (de
WO2009129797A4 (de
Inventor
Peter Bendzko
Hans Joos
Original Assignee
InViTek Gesellschaft für Biotechnik & Biodesign mbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by InViTek Gesellschaft für Biotechnik & Biodesign mbH filed Critical InViTek Gesellschaft für Biotechnik & Biodesign mbH
Priority to US12/988,906 priority Critical patent/US20110092687A1/en
Priority to DE112009001513.1T priority patent/DE112009001513B4/de
Publication of WO2009129797A2 publication Critical patent/WO2009129797A2/de
Publication of WO2009129797A3 publication Critical patent/WO2009129797A3/de
Publication of WO2009129797A4 publication Critical patent/WO2009129797A4/de
Priority to US14/171,157 priority patent/US9593325B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Die Erfindung betrifft eine lagerstabile Lysepuffermixtur zur Isolierung von Nukleinsäuren aus biologischen, vorzugsweise diagnostischen Proben. Sie ist bevorzugt mit einer Extraktionskontrolle verbunden. Die der Erfindung zugrunde liegende Aufgabe ist es, ein verbessertes Nukleinsäureextraktionssystem bereitzustellen, welches kostengünstig, stabil und einfach anzuwenden ist und dabei den Ansprüchen an ein modernes Nukleinsäureextraktionssystem genügt, indem es u.a. Extraktionskontrollen enthält. Gegenstand der Erfindung ist eine Lysepuffermixtur zur Isolierung von Nukleinsäuren, welche nicht chaotrope Salze, eine besondere Auswahl an Detergenzien, eine definierte Menge mindestens einer Nukleinsäure als Extraktionskontrolle, gegebenenfalls lytische Enzyme, gegebenenfalls Carrier Nukleinsäuren sowie gegebenenfalls weitere Zusätze umfasst.
PCT/DE2009/000549 2008-04-22 2009-04-20 Stabile lysepuffermixtur zur extraktion von nukleinsäuren WO2009129797A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US12/988,906 US20110092687A1 (en) 2008-04-22 2009-04-20 Stable lysis buffer mixture for extracting nucleic acids
DE112009001513.1T DE112009001513B4 (de) 2008-04-22 2009-04-20 Stabile Lysepuffermixtur zur Extraktion von Nukleinsäuren
US14/171,157 US9593325B2 (en) 2008-04-22 2014-02-03 Stable lysis buffer mixture for extracting nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102008020258.4 2008-04-22
DE102008020258A DE102008020258A1 (de) 2008-04-22 2008-04-22 Stabile Lysepuffermixtur zur Extraktion von Nukleinsäuren

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/988,906 A-371-Of-International US20110092687A1 (en) 2008-04-22 2009-04-20 Stable lysis buffer mixture for extracting nucleic acids
US14/171,157 Continuation US9593325B2 (en) 2008-04-22 2014-02-03 Stable lysis buffer mixture for extracting nucleic acids

Publications (3)

Publication Number Publication Date
WO2009129797A2 WO2009129797A2 (de) 2009-10-29
WO2009129797A3 true WO2009129797A3 (de) 2009-12-30
WO2009129797A4 WO2009129797A4 (de) 2010-03-04

Family

ID=41111646

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2009/000549 WO2009129797A2 (de) 2008-04-22 2009-04-20 Stabile lysepuffermixtur zur extraktion von nukleinsäuren

Country Status (3)

Country Link
US (2) US20110092687A1 (de)
DE (2) DE102008020258A1 (de)
WO (1) WO2009129797A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011054474B4 (de) 2011-07-20 2014-02-13 Stratec Biomedical Ag System zur Stabilisierung, Aufbewahrung und Lagerung einer Nukleinsäure
JP6317353B2 (ja) 2012-09-13 2018-04-25 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 固形組織の単細胞における多重分析測定のための方法
JP6767374B2 (ja) 2014-10-20 2020-10-14 ジェン−プローブ・インコーポレーテッド 赤血球溶解溶液
DE202017007129U1 (de) 2016-04-27 2019-08-29 Gen-Probe Incorporated Lysereagenz für Blutzellen
WO2018063772A1 (en) * 2016-09-27 2018-04-05 Abbott Molecular Inc. Maximizing dna yield of blood specimens collected in rapid clot tubes
US11647993B2 (en) * 2017-12-22 2023-05-16 Research Triangle Institute Oral fluid collector
CN111778236A (zh) * 2020-06-23 2020-10-16 辽宁省海洋水产科学研究院 基于3d打印特形功能体的贝类基因组dna提取方法、试剂盒及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003040386A2 (de) * 2001-11-02 2003-05-15 InViTek Gesellschaft für Biotechnik & Biodesign mbH Reaktionsräume enthaltend komplexe lagerstabile reagenzienformulierungen und testkit zum nachweis und zur isolierung pathogener mikrobieller nukleinsäuren
EP1462520A1 (de) * 2003-03-28 2004-09-29 Eppendorf 5 Prime, Inc. Verfahren zur Isolierung von DNA
EP1529840A1 (de) * 2003-11-04 2005-05-11 Qiagen GmbH Ein schnelles und preiswertes Verfahren zur Gewinnung von Nukleinsäuren
DE102006019650A1 (de) * 2006-04-25 2007-10-31 InViTek Gesellschaft für Biotechnik & Biodesign mbH Formulierungen und Verfahren zur Isolierung von Nukleinsäuren aus beliebigen komplexen Ausgangsmaterialien und nachfolgende komplexe Genanalytik

Family Cites Families (8)

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Publication number Priority date Publication date Assignee Title
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
WO1999040098A1 (de) * 1998-02-04 1999-08-12 Merck Patent Gmbh Verfahren zur isolierung und aufreinigung von nucleinsäuren
DE19840531C2 (de) 1998-08-28 2003-05-15 Roboscreen Ges Fuer Molekulare Mit Nukleinsäuren beschichtete Reaktionsräume, Verfahren zu ihrer Herstellung und ihre Verwendung
DE19856064C2 (de) 1998-12-04 2000-11-30 Invitek Gmbh Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien
EP1210358A4 (de) * 1999-08-13 2005-01-05 Univ Brandeis Nachweis von nukleinsäuren
US8852862B2 (en) * 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US20060223071A1 (en) * 2005-04-01 2006-10-05 Wisniewski Michele E Methods, compositions, and kits for detecting nucleic acids in a single vessel
US8187557B2 (en) * 2006-07-13 2012-05-29 Cepheid Reagent reservoir system for analytical instruments

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003040386A2 (de) * 2001-11-02 2003-05-15 InViTek Gesellschaft für Biotechnik & Biodesign mbH Reaktionsräume enthaltend komplexe lagerstabile reagenzienformulierungen und testkit zum nachweis und zur isolierung pathogener mikrobieller nukleinsäuren
EP1462520A1 (de) * 2003-03-28 2004-09-29 Eppendorf 5 Prime, Inc. Verfahren zur Isolierung von DNA
EP1529840A1 (de) * 2003-11-04 2005-05-11 Qiagen GmbH Ein schnelles und preiswertes Verfahren zur Gewinnung von Nukleinsäuren
DE102006019650A1 (de) * 2006-04-25 2007-10-31 InViTek Gesellschaft für Biotechnik & Biodesign mbH Formulierungen und Verfahren zur Isolierung von Nukleinsäuren aus beliebigen komplexen Ausgangsmaterialien und nachfolgende komplexe Genanalytik

Also Published As

Publication number Publication date
DE112009001513A5 (de) 2011-04-07
WO2009129797A2 (de) 2009-10-29
WO2009129797A4 (de) 2010-03-04
US9593325B2 (en) 2017-03-14
DE102008020258A1 (de) 2009-10-29
US20140154776A1 (en) 2014-06-05
US20110092687A1 (en) 2011-04-21
DE112009001513B4 (de) 2016-03-17

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