WO2009075376A1 - 抗オフロキサシンモノクローナル抗体、これを用いたオフロキサシンの免疫学的測定方法 - Google Patents

抗オフロキサシンモノクローナル抗体、これを用いたオフロキサシンの免疫学的測定方法 Download PDF

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Publication number
WO2009075376A1
WO2009075376A1 PCT/JP2008/072783 JP2008072783W WO2009075376A1 WO 2009075376 A1 WO2009075376 A1 WO 2009075376A1 JP 2008072783 W JP2008072783 W JP 2008072783W WO 2009075376 A1 WO2009075376 A1 WO 2009075376A1
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WO
WIPO (PCT)
Prior art keywords
ofloxacin
formula
compound represented
immunoassay method
same
Prior art date
Application number
PCT/JP2008/072783
Other languages
English (en)
French (fr)
Inventor
Osamu Miyazaki
Kohei Takubo
Isamu Fukamachi
Eriko Kusaka
Mieko Kashiwagi
Original Assignee
Sekisui Medical Co., Ltd.
Daiichi Sankyo Company, Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sekisui Medical Co., Ltd., Daiichi Sankyo Company, Limited filed Critical Sekisui Medical Co., Ltd.
Priority to JP2009525846A priority Critical patent/JP4494522B2/ja
Priority to CN2008801267510A priority patent/CN101945894A/zh
Priority to EP08859171A priority patent/EP2233503A4/en
Priority to US12/747,754 priority patent/US20100310597A1/en
Publication of WO2009075376A1 publication Critical patent/WO2009075376A1/ja

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9446—Antibacterials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/33—Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

【課題】ヒトの試料中の式（I）で示される化合物を正確かつ簡便に検出する方法を提供する。 【解決手段】式（I）で示される化合物を認識し、且つその代謝物である式（I）で示される化合物のＮ-オキシド体及びレボフロキサシン脱メチル体と交差反応しない抗体を作成するため、式（I）で示される化合物の６位のカルボキシ基にウシ血清アルブミンを結合させた抗原を用いた。また、代謝されていない式（I）で示される化合物を測定するイムノアッセイ法を構築するため、前記抗体を用いた。
PCT/JP2008/072783 2007-12-13 2008-12-15 抗オフロキサシンモノクローナル抗体、これを用いたオフロキサシンの免疫学的測定方法 WO2009075376A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2009525846A JP4494522B2 (ja) 2007-12-13 2008-12-15 抗オフロキサシンモノクローナル抗体、これを用いたオフロキサシンの免疫学的測定方法
CN2008801267510A CN101945894A (zh) 2007-12-13 2008-12-15 抗氧氟沙星单克隆抗体和使用其的氧氟沙星免疫测定
EP08859171A EP2233503A4 (en) 2007-12-13 2008-12-15 MONOCLONAL ANTI-OFLOXACIN ANTIBODIES AND IMMUNASSAY METHOD ON OFLOXACIN USING THEREOF
US12/747,754 US20100310597A1 (en) 2007-12-13 2008-12-15 Anti-ofloxacin monoclonal antibody and immunoassay of ofloxacin using the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-321529 2007-12-13
JP2007321529 2007-12-13

Publications (1)

Publication Number Publication Date
WO2009075376A1 true WO2009075376A1 (ja) 2009-06-18

Family

ID=40755609

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/072783 WO2009075376A1 (ja) 2007-12-13 2008-12-15 抗オフロキサシンモノクローナル抗体、これを用いたオフロキサシンの免疫学的測定方法

Country Status (6)

Country Link
US (1) US20100310597A1 (ja)
EP (1) EP2233503A4 (ja)
JP (1) JP4494522B2 (ja)
KR (1) KR20100107466A (ja)
CN (1) CN101945894A (ja)
WO (1) WO2009075376A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102269765A (zh) * 2011-06-13 2011-12-07 清华大学深圳研究生院 一种检测恩诺沙星的免疫层析试纸及其制备方法
JP2023009891A (ja) * 2021-07-08 2023-01-20 学校法人九州文化学園 血中レボフロキサシン濃度測定方法

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6216926B2 (ja) 2012-08-21 2017-10-25 ヤンセン ファーマシューティカ エヌ．ベー． オランザピンに対する抗体及びその使用
CN104017068B (zh) * 2014-06-16 2016-06-08 宁波艾科生物科技有限公司 一种快速制备多种抗抗生素杂交瘤单克隆抗体的方法
CN108727502A (zh) * 2018-05-02 2018-11-02 华南农业大学 一种左旋氧氟沙星抗体的Fab片段制备方法及应用
CN110412260A (zh) * 2019-04-26 2019-11-05 山东省食品药品检验研究院 化妆品中氟喹诺酮类药物直接竞争化学发光定性定量免疫分析方法
CN110563804A (zh) * 2019-09-19 2019-12-13 河南省农业科学院 基于天然免疫库筛选识别氧氟沙星单链抗体的多肽序列及应用
CN110498839A (zh) * 2019-09-19 2019-11-26 河南省农业科学院 氟罗沙星单链抗体关键多肽序列及应用
CN112946254B (zh) * 2021-01-18 2024-03-15 上海云泽生物科技有限公司 一种胶乳增强竞争免疫比浊检测方法及试剂盒
CN113121389A (zh) * 2021-04-27 2021-07-16 广东省药品检验所（广东省药品质量研究所、广东省口岸药品检验所） 一种苯胺类药物快速检测试剂盒及其制备方法与应用
CN114437203B (zh) * 2022-01-20 2024-03-26 杭州市农业科学研究院 一种氧氟沙星抗原的合成方法及其在制备单克隆抗体中的用途

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07267999A (ja) 1994-03-29 1995-10-17 Dai Ichi Seiyaku Co Ltd 抗菌化合物の免疫学的測定法
JP2007063180A (ja) 2005-08-31 2007-03-15 Frontier Kenkyusho:Kk ニューキノロン系抗菌剤の検出方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100478357C (zh) * 2006-03-14 2009-04-15 山东大学 氧氟沙星的偶联物的制备方法
CN1974600B (zh) * 2006-10-27 2010-05-12 华南农业大学 抗氟甲喹的单克隆抗体、其制备方法及其应用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07267999A (ja) 1994-03-29 1995-10-17 Dai Ichi Seiyaku Co Ltd 抗菌化合物の免疫学的測定法
JP2007063180A (ja) 2005-08-31 2007-03-15 Frontier Kenkyusho:Kk ニューキノロン系抗菌剤の検出方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
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WANG Z ET AL.: "Development of a monoclonal antibody-based broad-specificity ELISA for fluoroquinolone antibiotics in foods and molecular modeling studies of cross-reactive compounds.", ANAL CHEM., vol. 79, no. 12, June 2007 (2007-06-01), pages 4471 - 4483, XP008137443 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102269765A (zh) * 2011-06-13 2011-12-07 清华大学深圳研究生院 一种检测恩诺沙星的免疫层析试纸及其制备方法
CN102269765B (zh) * 2011-06-13 2014-03-19 清华大学深圳研究生院 一种检测恩诺沙星的免疫层析试纸及其制备方法
JP2023009891A (ja) * 2021-07-08 2023-01-20 学校法人九州文化学園 血中レボフロキサシン濃度測定方法
JP7365063B2 (ja) 2021-07-08 2023-10-19 学校法人九州文化学園 血中レボフロキサシン濃度測定方法

Also Published As

Publication number Publication date
JPWO2009075376A1 (ja) 2011-04-28
JP4494522B2 (ja) 2010-06-30
US20100310597A1 (en) 2010-12-09
CN101945894A (zh) 2011-01-12
EP2233503A4 (en) 2011-08-03
EP2233503A1 (en) 2010-09-29
KR20100107466A (ko) 2010-10-05

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