WO2009072457A1 - 被覆磁性微粒子を用いたバイオセンシング方法及び該方法に用いるバイオセンシング装置 - Google Patents

被覆磁性微粒子を用いたバイオセンシング方法及び該方法に用いるバイオセンシング装置 Download PDF

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Publication number
WO2009072457A1
WO2009072457A1 PCT/JP2008/071780 JP2008071780W WO2009072457A1 WO 2009072457 A1 WO2009072457 A1 WO 2009072457A1 JP 2008071780 W JP2008071780 W JP 2008071780W WO 2009072457 A1 WO2009072457 A1 WO 2009072457A1
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WIPO (PCT)
Prior art keywords
biosensing
magnetic microparticles
magnetic
affinity reaction
reaction
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PCT/JP2008/071780
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English (en)
French (fr)
Inventor
Hiroshi Handa
Masanori Abe
Mamoru Hatakeyama
Satoshi Sakamoto
Kosuke Nishio
Yusuke Mochizuki
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Tokyo Institute Of Technology
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Application filed by Tokyo Institute Of Technology filed Critical Tokyo Institute Of Technology
Priority to EP08857449.6A priority Critical patent/EP2246702B1/en
Priority to US12/745,619 priority patent/US20100323457A1/en
Priority to JP2009544655A priority patent/JP5401724B2/ja
Priority to CN200880125276.5A priority patent/CN101952725B/zh
Publication of WO2009072457A1 publication Critical patent/WO2009072457A1/ja

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55—Specular reflectivity
    • G01N21/552—Attenuated total reflection
    • G01N21/553—Attenuated total reflection and using surface plasmons

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

　本発明は、バイオセンシングにおける磁性粒子の新たな応用展開として、その標識としての優位性を生かしながら、センシングのスループットを同時に改善する、新規なバイオセンシング方法を提供することを目的とする。バイオセンシングにおけるアフィニティ反応において、リガンドを磁性微粒子に固定化し、当該磁性微粒子をアフィニティ反応の反応場に向けて強制的に磁気誘導することによって、センシングにおける律速因子であるアフィニティ反応を迅速化する。本発明においては、上記磁性微粒子として、高分散性と高い磁気応答性の両方を併せ持つことを特徴とした被覆磁性微粒子を採用する。本発明によれば、アフィニティ反応が迅速且つ高密度に発生し、その結果、従来に比べて格段に短い時間で大きなシグナルを得ることができる。
PCT/JP2008/071780 2007-12-03 2008-12-01 被覆磁性微粒子を用いたバイオセンシング方法及び該方法に用いるバイオセンシング装置 WO2009072457A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP08857449.6A EP2246702B1 (en) 2007-12-03 2008-12-01 Biosensing method using coated magnetic microparticles and biosensing device to be used in the method
US12/745,619 US20100323457A1 (en) 2007-12-03 2008-12-01 Biosensing method using coated magnetic fine particles and biosensing apparatus for biosensing method
JP2009544655A JP5401724B2 (ja) 2007-12-03 2008-12-01 被覆磁性微粒子を用いたバイオセンシング方法及び該方法に用いるバイオセンシング装置
CN200880125276.5A CN101952725B (zh) 2007-12-03 2008-12-01 使用了包覆磁性微粒的生物传感方法以及用于该方法的生物传感装置

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-311860 2007-12-03
JP2007311860 2007-12-03

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WO2009072457A1 true WO2009072457A1 (ja) 2009-06-11

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Country Status (5)

Country Link
US (1) US20100323457A1 (ja)
EP (1) EP2246702B1 (ja)
JP (1) JP5401724B2 (ja)
CN (1) CN101952725B (ja)
WO (1) WO2009072457A1 (ja)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012215553A (ja) * 2011-03-30 2012-11-08 Toshiba Corp 光導波路型測定システム、測定方法及び光導波路型センサチップ
WO2013080491A1 (ja) * 2011-11-29 2013-06-06 国立大学法人東京工業大学 機能性物質包含微粒子を用いたバイオセンシング方法
JP2013238541A (ja) * 2012-05-16 2013-11-28 Toshiba Corp 光導波路型測定システムおよび糖化ヘモグロビンの測定方法
JP2015025820A (ja) * 2014-11-04 2015-02-05 株式会社東芝 光導波路型測定システムおよび糖化ヘモグロビンの測定方法
US9274104B2 (en) 2011-03-30 2016-03-01 Kabushiki Kaisha Toshiba Measuring system using optical waveguide, measuring device, measuring method, optical waveguide type sensor chip, and magnetic fine particle
JP6370018B1 (ja) * 2018-03-15 2018-08-08 株式会社 マトリックス細胞研究所 反応促進装置及び反応促進方法
JP6370017B1 (ja) * 2018-03-15 2018-08-08 株式会社 マトリックス細胞研究所 反応促進装置及び反応促進方法
JP6420433B1 (ja) * 2017-09-12 2018-11-07 株式会社 マトリックス細胞研究所 反応促進装置及び反応促進方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010029739A1 (ja) * 2008-09-11 2010-03-18 国立大学法人東京工業大学 蛍光分子を含有するポリマー粒子およびその製造方法
CN104165819B (zh) * 2013-08-29 2016-06-08 北京至感传感器技术研究院有限公司 一种在线实时磁性颗粒监测系统

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05203651A (ja) * 1991-07-22 1993-08-10 Pasteur Sanofi Diagnostics 固相上で免疫学的分析を行うための磁気的方法及び装置
JP2001510565A (ja) * 1997-01-30 2001-07-31 ベーイーオー メリュー 媒体中の分析物を単離、特には検出又は定量する方法
JP2003130880A (ja) 2001-10-29 2003-05-08 Fujirebio Inc 異常型プリオン免疫測定用試薬及びキット並びにそれを用いた異常型プリオンの免疫測定方法
JP2005533236A (ja) 2001-09-20 2005-11-04 ボード　オブ　リージェンツ，　ザ　ユニバーシティ　オブ　テキサス　システム Ｅｌｉｓａアッセイを用いた循環する治療抗体、抗原および抗原／抗体複合体の測定
JP2006088131A (ja) 2004-09-27 2006-04-06 Rikogaku Shinkokai ポリマー被覆強磁性粒子の製造方法及びポリマー被覆強磁性粒子
WO2006079016A1 (en) * 2005-01-20 2006-07-27 Luminex Corporation Magnetic microspheres for use in fluorescence-based applications
JP2006313493A (ja) 2005-05-09 2006-11-16 Mitsubishi Electric Corp 電源回路
JP2007194233A (ja) 2006-01-17 2007-08-02 Kitashiba Electric Co Ltd 三相変圧器の輸送及び現地組立方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04102062A (ja) * 1990-08-22 1992-04-03 Hitachi Ltd 粒子免疫側定法
US6586193B2 (en) * 1996-04-25 2003-07-01 Genicon Sciences Corporation Analyte assay using particulate labels
FR2749082B1 (fr) * 1996-05-24 1998-06-26 Bio Merieux Particules superparamagnetiques et monodispersees
US6309701B1 (en) * 1998-11-10 2001-10-30 Bio-Pixels Ltd. Fluorescent nanocrystal-labeled microspheres for fluorescence analyses
CN1185492C (zh) * 1999-03-15 2005-01-19 清华大学 可单点选通式微电磁单元阵列芯片、电磁生物芯片及应用
FR2832507B1 (fr) * 2001-11-20 2004-02-20 Stago Diagnostica Methode de detection d'analyte(s) a l'aide de particules magnetiques colloidales
US7048890B2 (en) * 2001-12-21 2006-05-23 Koninklijke Philips Electronics N.V. Sensor and method for measuring the areal density of magnetic nanoparticles on a micro-array
CN100442052C (zh) * 2002-08-15 2008-12-10 陕西西大北美基因股份有限公司 磁性荧光微球及其制备方法和采用该磁性荧光微球进行生物分子检测的方法
US7235228B2 (en) * 2003-04-15 2007-06-26 The United States Of America As Represented By The Secretary Of The Navy Fluorescent-magnetic nanoparticles with core-shell structure
CN1280428C (zh) * 2003-05-19 2006-10-18 清华大学 一种基于微小颗粒的生物芯片系统及其应用
CN1524925A (zh) * 2003-09-18 2004-09-01 吉林大学 荧光磁性纳米复合物及其制备方法和应用
US20060257958A1 (en) * 2005-05-13 2006-11-16 Pronucleotein Biotechnologies, Llc Magnetically-assisted test strip cartridge and method for using same
JP4587903B2 (ja) * 2005-07-29 2010-11-24 シチズンホールディングス株式会社 測定器具及びこれを用いた測定用キット、測定方法並びに測定装置
TWI293113B (en) * 2005-12-23 2008-02-01 Ind Tech Res Inst Magnetic nanoparticles with fluorescent and specific targeting functions
US8586385B2 (en) * 2006-12-28 2013-11-19 Intel Corporation Method and device for biomolecule preparation and detection using magnetic array

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05203651A (ja) * 1991-07-22 1993-08-10 Pasteur Sanofi Diagnostics 固相上で免疫学的分析を行うための磁気的方法及び装置
JP2001510565A (ja) * 1997-01-30 2001-07-31 ベーイーオー メリュー 媒体中の分析物を単離、特には検出又は定量する方法
JP2005533236A (ja) 2001-09-20 2005-11-04 ボード　オブ　リージェンツ，　ザ　ユニバーシティ　オブ　テキサス　システム Ｅｌｉｓａアッセイを用いた循環する治療抗体、抗原および抗原／抗体複合体の測定
JP2003130880A (ja) 2001-10-29 2003-05-08 Fujirebio Inc 異常型プリオン免疫測定用試薬及びキット並びにそれを用いた異常型プリオンの免疫測定方法
JP2006088131A (ja) 2004-09-27 2006-04-06 Rikogaku Shinkokai ポリマー被覆強磁性粒子の製造方法及びポリマー被覆強磁性粒子
WO2006079016A1 (en) * 2005-01-20 2006-07-27 Luminex Corporation Magnetic microspheres for use in fluorescence-based applications
JP2006313493A (ja) 2005-05-09 2006-11-16 Mitsubishi Electric Corp 電源回路
JP2007194233A (ja) 2006-01-17 2007-08-02 Kitashiba Electric Co Ltd 三相変圧器の輸送及び現地組立方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2246702A4

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012215553A (ja) * 2011-03-30 2012-11-08 Toshiba Corp 光導波路型測定システム、測定方法及び光導波路型センサチップ
US9274104B2 (en) 2011-03-30 2016-03-01 Kabushiki Kaisha Toshiba Measuring system using optical waveguide, measuring device, measuring method, optical waveguide type sensor chip, and magnetic fine particle
WO2013080491A1 (ja) * 2011-11-29 2013-06-06 国立大学法人東京工業大学 機能性物質包含微粒子を用いたバイオセンシング方法
JP2013113735A (ja) * 2011-11-29 2013-06-10 Tokyo Institute Of Technology 機能性物質包含微粒子を用いたバイオセンシング方法
JP2013238541A (ja) * 2012-05-16 2013-11-28 Toshiba Corp 光導波路型測定システムおよび糖化ヘモグロビンの測定方法
JP2015025820A (ja) * 2014-11-04 2015-02-05 株式会社東芝 光導波路型測定システムおよび糖化ヘモグロビンの測定方法
WO2019054357A1 (ja) * 2017-09-12 2019-03-21 株式会社 マトリックス細胞研究所 反応促進装置及び反応促進方法
JP6420433B1 (ja) * 2017-09-12 2018-11-07 株式会社 マトリックス細胞研究所 反応促進装置及び反応促進方法
JP2019052851A (ja) * 2017-09-12 2019-04-04 株式会社 マトリックス細胞研究所 反応促進装置及び反応促進方法
JP6370017B1 (ja) * 2018-03-15 2018-08-08 株式会社 マトリックス細胞研究所 反応促進装置及び反応促進方法
JP6370018B1 (ja) * 2018-03-15 2018-08-08 株式会社 マトリックス細胞研究所 反応促進装置及び反応促進方法
JP2019053027A (ja) * 2018-03-15 2019-04-04 株式会社 マトリックス細胞研究所 反応促進装置及び反応促進方法
JP2019053026A (ja) * 2018-03-15 2019-04-04 株式会社 マトリックス細胞研究所 反応促進装置及び反応促進方法

Also Published As

Publication number Publication date
EP2246702A4 (en) 2011-09-28
US20100323457A1 (en) 2010-12-23
JP5401724B2 (ja) 2014-01-29
EP2246702B1 (en) 2015-10-07
JPWO2009072457A1 (ja) 2011-04-21
CN101952725B (zh) 2014-12-10
CN101952725A (zh) 2011-01-19
EP2246702A1 (en) 2010-11-03

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