WO2009037838A1 - 酵素電極 - Google Patents

酵素電極 Download PDF

Info

Publication number
WO2009037838A1
WO2009037838A1 PCT/JP2008/002573 JP2008002573W WO2009037838A1 WO 2009037838 A1 WO2009037838 A1 WO 2009037838A1 JP 2008002573 W JP2008002573 W JP 2008002573W WO 2009037838 A1 WO2009037838 A1 WO 2009037838A1
Authority
WO
WIPO (PCT)
Prior art keywords
carbon particles
enzyme electrode
electrode layer
electrode
gdh
Prior art date
Application number
PCT/JP2008/002573
Other languages
English (en)
French (fr)
Inventor
Wakako Tsugawa
Koji Sode
Original Assignee
Tokyo University Of Agriculture And Technology
Ultizyme International Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tokyo University Of Agriculture And Technology, Ultizyme International Ltd. filed Critical Tokyo University Of Agriculture And Technology
Priority to CA2699823A priority Critical patent/CA2699823C/en
Priority to US12/678,268 priority patent/US9617576B2/en
Priority to JP2009533048A priority patent/JP5273680B2/ja
Priority to ES08832471.0T priority patent/ES2441361T3/es
Priority to MX2010002962A priority patent/MX2010002962A/es
Priority to RU2010115275/04A priority patent/RU2476869C2/ru
Priority to CN200880107747XA priority patent/CN101802597B/zh
Priority to EP08832471.0A priority patent/EP2192402B1/en
Publication of WO2009037838A1 publication Critical patent/WO2009037838A1/ja
Priority to US15/444,070 priority patent/US20170191105A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3273—Devices therefor, e.g. test element readers, circuitry

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Emergency Medicine (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Inert Electrodes (AREA)

Abstract

本発明は、フラビンアデニンジヌクレオチド（ＦＡＤ）を補酵素とするグルコース脱水素酵素（ＧＤＨ）が担持された炭素粒子と、前記炭素粒子と接する電極層から構成される酵素電極において、前記炭素粒子及び／又は前記電極層が、粒径１００ｎｍ以下でかつ比表面積が少なくとも２００ｍ2／ｇ以上である炭素粒子から構成されていることを特徴とする酵素電極を提供する。
PCT/JP2008/002573 2007-09-18 2008-09-18 酵素電極 WO2009037838A1 (ja)

Priority Applications (9)

Application Number Priority Date Filing Date Title
CA2699823A CA2699823C (en) 2007-09-18 2008-09-18 Enzyme electrode
US12/678,268 US9617576B2 (en) 2007-09-18 2008-09-18 Enzyme electrode
JP2009533048A JP5273680B2 (ja) 2007-09-18 2008-09-18 酵素電極
ES08832471.0T ES2441361T3 (es) 2007-09-18 2008-09-18 Electrodo enzimático
MX2010002962A MX2010002962A (es) 2007-09-18 2008-09-18 Un electrodo de enzima.
RU2010115275/04A RU2476869C2 (ru) 2007-09-18 2008-09-18 Ферментный электрод
CN200880107747XA CN101802597B (zh) 2007-09-18 2008-09-18 酶电极
EP08832471.0A EP2192402B1 (en) 2007-09-18 2008-09-18 Enzyme electrode
US15/444,070 US20170191105A1 (en) 2007-09-18 2017-02-27 Enzyme electrode

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007-240812 2007-09-18
JP2007240812 2007-09-18
JP2008-124741 2008-05-12
JP2008124741 2008-05-12

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/678,268 A-371-Of-International US9617576B2 (en) 2007-09-18 2008-09-18 Enzyme electrode
US15/444,070 Continuation US20170191105A1 (en) 2007-09-18 2017-02-27 Enzyme electrode

Publications (1)

Publication Number Publication Date
WO2009037838A1 true WO2009037838A1 (ja) 2009-03-26

Family

ID=40467665

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/002573 WO2009037838A1 (ja) 2007-09-18 2008-09-18 酵素電極

Country Status (9)

Country Link
US (2) US9617576B2 (ja)
EP (2) EP2679990B1 (ja)
JP (1) JP5273680B2 (ja)
CN (2) CN103472108B (ja)
CA (1) CA2699823C (ja)
ES (2) ES2441361T3 (ja)
MX (1) MX2010002962A (ja)
RU (1) RU2476869C2 (ja)
WO (1) WO2009037838A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010126139A1 (ja) 2009-04-30 2010-11-04 池田食研株式会社 蛋白質性電子メディエータ
WO2014002998A1 (ja) 2012-06-25 2014-01-03 合同会社バイオエンジニアリング研究所 酵素電極
WO2019172400A1 (ja) 2018-03-08 2019-09-12 有限会社アルティザイム・インターナショナル フラビンアデニンジヌクレオチドグルコース脱水素酵素とシトクロム分子との融合タンパク質

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5616235B2 (ja) 2009-02-09 2014-10-29 アークレイ株式会社 電気化学センサーおよびその作製方法
WO2014110498A1 (en) * 2013-01-11 2014-07-17 Stc.Unm Highly efficient enzymatic bioanodes and biocathodes
ES2484665B2 (es) * 2013-02-11 2015-03-16 Universidad De Burgos Dispositivo electródico para la detección de ácido glucónico, procedimiento de fabricación y uso de dicho dispositivo
WO2019189808A1 (ja) 2018-03-29 2019-10-03 東洋紡株式会社 ナノカーボンの電子伝達作用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005502045A (ja) * 2001-08-28 2005-01-20 デューク・ユニバーシティー バイオセンサー
WO2005121355A1 (en) * 2004-06-04 2005-12-22 Medtronic Minimed, Inc. Analyte sensors and methods for making and using them
WO2007055100A1 (ja) * 2005-11-08 2007-05-18 Ultizyme International Ltd. 酵素電極
JP2007218795A (ja) * 2006-02-17 2007-08-30 Toyota Central Res & Dev Lab Inc 電極材料、並びにそれを用いたバイオセンサー及び燃料電池

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3669864A (en) * 1969-09-02 1972-06-13 Robert R Fike Polarographic electrode apparatus and method
SE418781B (sv) * 1977-12-09 1981-06-22 Asea Ab Sett vid tillverkning av en starkstromskabel
GB8612861D0 (en) * 1986-05-27 1986-07-02 Cambridge Life Sciences Immobilised enzyme biosensors
GB8710472D0 (en) 1987-05-01 1987-06-03 Cambridge Life Sciences Amperometric method
GB8817997D0 (en) * 1988-07-28 1988-09-01 Cambridge Life Sciences Enzyme electrodes & improvements in manufacture thereof
US5755953A (en) * 1995-12-18 1998-05-26 Abbott Laboratories Interference free biosensor
US6475372B1 (en) * 2000-02-02 2002-11-05 Lifescan, Inc. Electrochemical methods and devices for use in the determination of hematocrit corrected analyte concentrations
AU7590300A (en) * 1999-09-20 2001-04-24 Roche Diagnostics Corporation Small volume biosensor for continuous analyte monitoring
CN1198138C (zh) * 2000-09-25 2005-04-20 旭化成株式会社 酶电极
CA2427031C (en) 2000-10-31 2016-02-09 Koji Sode Novel glucose dehydrogenase and method for producing the dehydrogenase
WO2002073181A1 (fr) 2001-03-13 2002-09-19 Koji Sode Electrode enzymatique
JP3862540B2 (ja) 2001-10-12 2006-12-27 日本電信電話株式会社 ナノ金属微粒子含有炭素薄膜電極及びその製造方法
US7291256B2 (en) * 2002-09-12 2007-11-06 Lifescan, Inc. Mediator stabilized reagent compositions and methods for their use in electrochemical analyte detection assays
US7638228B2 (en) * 2002-11-27 2009-12-29 Saint Louis University Enzyme immobilization for use in biofuel cells and sensors
EP1661516B1 (en) * 2003-09-02 2012-08-15 Koji Sode Glucose sensor and glucose level measuring apparatus
WO2005040407A1 (en) * 2003-10-24 2005-05-06 Bayer Healthcare Llc Enzymatic electrochemical biosensor
WO2005093400A1 (ja) 2004-03-25 2005-10-06 Ultizyme International Ltd. 燃料電池型ワイヤレス酵素センサー
US7955484B2 (en) * 2005-12-14 2011-06-07 Nova Biomedical Corporation Glucose biosensor and method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005502045A (ja) * 2001-08-28 2005-01-20 デューク・ユニバーシティー バイオセンサー
WO2005121355A1 (en) * 2004-06-04 2005-12-22 Medtronic Minimed, Inc. Analyte sensors and methods for making and using them
WO2007055100A1 (ja) * 2005-11-08 2007-05-18 Ultizyme International Ltd. 酵素電極
JP2007218795A (ja) * 2006-02-17 2007-08-30 Toyota Central Res & Dev Lab Inc 電極材料、並びにそれを用いたバイオセンサー及び燃料電池

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2192402A4 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010126139A1 (ja) 2009-04-30 2010-11-04 池田食研株式会社 蛋白質性電子メディエータ
EP2589659A1 (en) 2009-04-30 2013-05-08 Ikeda Food Research Co. Ltd. Protein-type electron mediator
US8716442B2 (en) 2009-04-30 2014-05-06 Ikeda Food Research Co., Ltd. Protein electron mediator
US8969025B2 (en) 2009-04-30 2015-03-03 Panasonic Healthcare Holdings Co., Ltd. Protein electron mediator
WO2014002998A1 (ja) 2012-06-25 2014-01-03 合同会社バイオエンジニアリング研究所 酵素電極
WO2019172400A1 (ja) 2018-03-08 2019-09-12 有限会社アルティザイム・インターナショナル フラビンアデニンジヌクレオチドグルコース脱水素酵素とシトクロム分子との融合タンパク質

Also Published As

Publication number Publication date
EP2192402A1 (en) 2010-06-02
ES2441361T3 (es) 2014-02-04
CN103472108A (zh) 2013-12-25
EP2679990B1 (en) 2015-11-04
US20170191105A1 (en) 2017-07-06
CA2699823A1 (en) 2009-03-26
MX2010002962A (es) 2010-03-31
EP2192402B1 (en) 2013-11-27
EP2679990A1 (en) 2014-01-01
US20100261072A1 (en) 2010-10-14
CN101802597A (zh) 2010-08-11
RU2010115275A (ru) 2011-10-27
CA2699823C (en) 2016-10-25
ES2555295T3 (es) 2015-12-30
US9617576B2 (en) 2017-04-11
EP2192402A4 (en) 2010-09-01
CN103472108B (zh) 2017-09-19
CN101802597B (zh) 2013-08-21
JP5273680B2 (ja) 2013-08-28
JPWO2009037838A1 (ja) 2011-01-06
RU2476869C2 (ru) 2013-02-27

Similar Documents

Publication Publication Date Title
WO2009037838A1 (ja) 酵素電極
WO2010051527A8 (en) Engineered microorganisms capable of producing target compounds under anaerobic conditions
WO2011012270A8 (en) Enzymatic synthesis of carba-nad
WO2009145798A3 (en) Methods for modifying features of a workpiece using a gas cluster ion beam
WO2008054349A3 (en) Carbon nanotube structures formed on large free floating substrates
WO2004038401A3 (en) Biosensor having hematocrit and oxygen biases
WO2008117630A1 (ja) 酵素固定化電極、燃料電池、電子機器、酵素反応利用装置および酵素固定化基体
WO2013188844A3 (en) Bioconversion processes and apparatus
WO2009047498A3 (en) Mutant enzymes
WO2008033167A3 (en) Nano-particle biochip substrates
WO2012071392A8 (en) Engineering of thermotolerant bacillus coagulans for production of d(-)-lactic acid
EA200702153A1 (ru) Термофильные микроорганизмы с инактивированным геном лактатдегидрогеназы (ldh) для производства этанола
WO2008082693A3 (en) Biomolecular hybrid material and process for preparing same and uses for same
GB0612094D0 (en) Electrode, method of manufacture and use thereof
WO2008093847A1 (ja) キシリトールデヒドロゲナーゼをコードするｄｎａ
WO2008045696A3 (en) Methods and compositions for the treatment of vaginal diseases employing peroxide-producing enzymes and peroxidases
WO2008153157A1 (ja) 酵素電極及び酵素センサ
WO2009133464A3 (en) Oxidation process
EP1367120A3 (en) Modified pyrroloquinoline quinone (PQQ) dependent glucose dehydrogenase with superior substrate specificity and stability
WO2013131942A3 (en) Mutated cellobiose dehydrogenase with increased substrate specificity
WO2006015802A3 (de) Verfahren zur herstellung von primären alkoholen durch reduktion von aldehyden unter verwendung einer alkoholdehydrogenase bei gekoppelter cofaktor-regenerierung
WO2009041415A1 (ja) 新規グルコース脱水素酵素
AU2003266255A1 (en) Method for amplification of nucleic acids of low complexity
JP2007529232A5 (ja)
WO2008131215A3 (en) Engineered phosphite dehydrogenase mutants

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 200880107747.X

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08832471

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 2009533048

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 12678268

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: MX/A/2010/002962

Country of ref document: MX

WWE Wipo information: entry into national phase

Ref document number: 2699823

Country of ref document: CA

Ref document number: 1504/CHENP/2010

Country of ref document: IN

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2008832471

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2010115275

Country of ref document: RU