WO2009022558A1 - 微生物検出法及び微生物検出キット - Google Patents

微生物検出法及び微生物検出キット Download PDF

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Publication number
WO2009022558A1
WO2009022558A1 PCT/JP2008/063865 JP2008063865W WO2009022558A1 WO 2009022558 A1 WO2009022558 A1 WO 2009022558A1 JP 2008063865 W JP2008063865 W JP 2008063865W WO 2009022558 A1 WO2009022558 A1 WO 2009022558A1
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WO
WIPO (PCT)
Prior art keywords
crosslinking agent
sample
light
microorganism detection
wavelength
Prior art date
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PCT/JP2008/063865
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English (en)
French (fr)
Inventor
Shinichi Yoshida
Takashi Soejima
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Kyushu University
Morinaga Milk Industry Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyushu University, Morinaga Milk Industry Co., Ltd. filed Critical Kyushu University
Priority to EP08792078.1A priority Critical patent/EP2077334B1/en
Priority to JP2008551589A priority patent/JP4378537B2/ja
Priority to CA2666448A priority patent/CA2666448C/en
Priority to NZ576113A priority patent/NZ576113A/en
Priority to AU2008287928A priority patent/AU2008287928B2/en
Priority to CN200880005230XA priority patent/CN101617057B/zh
Priority to US12/445,506 priority patent/US8221975B2/en
Publication of WO2009022558A1 publication Critical patent/WO2009022558A1/ja
Priority to US13/523,738 priority patent/US20120277121A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/101—Crosslinking agents, e.g. psoralen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/109—Invader technology

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

以下の工程により、被検試料中の微生物の生菌を検出する。 ａ）前記被検試料に、３５０ｎｍ～７００ｎｍの波長の光照射によりＤＮＡを架橋する架橋剤を添加する工程、 ｂ）架橋剤を添加した被検試料に３５０ｎｍ～７００ｎｍの波長の光照射処理を行う工程、 ｃ）光照射処理した被検試料に含まれる架橋剤を除去する工程、 ｄ）架橋剤を除去した被検試料に培地を添加して保温する工程、 ｅ）保温した被検試料に再び３５０ｎｍ～７００ｎｍの波長の光照射によりＤＮＡを架橋する架橋剤を添加する工程、 ｆ）架橋剤を添加した被検試料に３５０ｎｍ～７００ｎｍの波長の光照射処理を行う工程、 ｇ）前記被検試料からＤＮＡを抽出し、抽出されたＤＮＡのターゲット領域を核酸増幅法により増幅する工程、及び ｈ）増幅産物を解析する工程
PCT/JP2008/063865 2007-08-16 2008-08-01 微生物検出法及び微生物検出キット WO2009022558A1 (ja)

Priority Applications (8)

Application Number Priority Date Filing Date Title
EP08792078.1A EP2077334B1 (en) 2007-08-16 2008-08-01 Method and use of a kit for detection of microorganism
JP2008551589A JP4378537B2 (ja) 2007-08-16 2008-08-01 微生物検出法及び微生物検出キット
CA2666448A CA2666448C (en) 2007-08-16 2008-08-01 Method and kit for the detection of live microorganism cells in a sample
NZ576113A NZ576113A (en) 2007-08-16 2008-08-01 Microorganism detection method and microorganism detection kit
AU2008287928A AU2008287928B2 (en) 2007-08-16 2008-08-01 Method and kit for detection of microorganism
CN200880005230XA CN101617057B (zh) 2007-08-16 2008-08-01 微生物检测方法及试剂盒
US12/445,506 US8221975B2 (en) 2007-08-16 2008-08-01 Method for detection of microorganism
US13/523,738 US20120277121A1 (en) 2007-08-16 2012-06-14 Kit for detection of microorganism

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007212366 2007-08-16
JP2007-212366 2007-08-16

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/523,738 Division US20120277121A1 (en) 2007-08-16 2012-06-14 Kit for detection of microorganism

Publications (1)

Publication Number Publication Date
WO2009022558A1 true WO2009022558A1 (ja) 2009-02-19

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PCT/JP2008/063865 WO2009022558A1 (ja) 2007-08-16 2008-08-01 微生物検出法及び微生物検出キット

Country Status (10)

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US (2) US8221975B2 (ja)
EP (1) EP2077334B1 (ja)
JP (1) JP4378537B2 (ja)
KR (1) KR101120270B1 (ja)
CN (1) CN101617057B (ja)
AU (1) AU2008287928B2 (ja)
CA (1) CA2666448C (ja)
NZ (1) NZ576113A (ja)
RU (1) RU2410440C1 (ja)
WO (1) WO2009022558A1 (ja)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011010740A1 (ja) * 2009-07-24 2011-01-27 森永乳業株式会社 微生物検出法及び微生物検出キット
WO2012102208A1 (ja) 2011-01-24 2012-08-02 タカラバイオ株式会社 核酸修飾方法
CN103497999A (zh) * 2013-09-29 2014-01-08 杨波 一种用于肉毒杆菌lamp核酸检测引物组和试剂盒
CN109182469A (zh) * 2018-08-24 2019-01-11 暨南大学 基于数字lamp技术检测阪崎肠杆菌的引物及试剂盒与方法

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CN101341249B (zh) 2006-02-17 2013-07-24 森永乳业株式会社 用于检测微生物的方法和用于检测微生物的试剂盒
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RU2525934C1 (ru) * 2013-05-13 2014-08-20 Федеральное государственное бюджетное учреждение науки Институт экологии и генетики микроорганизмов Уральского отделения Российской академии наук Способ видовой дифференциации жизнеспособных родококков, иммобилизованных в гелевом носителе
RU2700247C1 (ru) * 2018-10-01 2019-09-13 Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" Тест-система для выявления ДНК сальмонелл (Salmonella spp.) в биологическом материале животных, продуктах питания и кормах животного и растительного происхождения
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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011010740A1 (ja) * 2009-07-24 2011-01-27 森永乳業株式会社 微生物検出法及び微生物検出キット
JP4825313B2 (ja) * 2009-07-24 2011-11-30 森永乳業株式会社 微生物検出法及び微生物検出キット
RU2527897C2 (ru) * 2009-07-24 2014-09-10 Моринага Милк Индастри Ко., Лтд. Способ и набор для детекции микроорганизмов
US9394572B2 (en) 2009-07-24 2016-07-19 Morinaga Milk Industry Co., Ltd. Method and kit for detection of microorganism
US10329604B2 (en) 2009-07-24 2019-06-25 Morinaga Milk Industry Co., Ltd. Method and kit for detection of microorganism
WO2012102208A1 (ja) 2011-01-24 2012-08-02 タカラバイオ株式会社 核酸修飾方法
US9458498B2 (en) 2011-01-24 2016-10-04 Takara Bio Inc. Method for modifying nucleic acids
US9670478B2 (en) 2011-01-24 2017-06-06 Takara Bio Inc. Method for modifying nucleic acids
EP3187598A1 (en) 2011-01-24 2017-07-05 Takara Bio Inc. Method for modifying nucleic acids
CN103497999A (zh) * 2013-09-29 2014-01-08 杨波 一种用于肉毒杆菌lamp核酸检测引物组和试剂盒
CN103497999B (zh) * 2013-09-29 2015-03-04 杨波 一种用于肉毒杆菌lamp核酸检测引物组和试剂盒
CN109182469A (zh) * 2018-08-24 2019-01-11 暨南大学 基于数字lamp技术检测阪崎肠杆菌的引物及试剂盒与方法

Also Published As

Publication number Publication date
AU2008287928A1 (en) 2009-02-19
EP2077334A4 (en) 2012-04-04
CN101617057A (zh) 2009-12-30
NZ576113A (en) 2012-05-25
AU2008287928B2 (en) 2011-07-28
US8221975B2 (en) 2012-07-17
CN101617057B (zh) 2013-10-30
RU2410440C1 (ru) 2011-01-27
KR20090078333A (ko) 2009-07-17
CA2666448A1 (en) 2009-02-19
EP2077334B1 (en) 2015-10-14
EP2077334A1 (en) 2009-07-08
CA2666448C (en) 2013-09-10
KR101120270B1 (ko) 2012-03-06
JP4378537B2 (ja) 2009-12-09
RU2009120527A (ru) 2010-12-10
US20120277121A1 (en) 2012-11-01
US20110053790A1 (en) 2011-03-03
JPWO2009022558A1 (ja) 2010-11-11

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