WO2008129072A1 - Dispositif de test multipuits - Google Patents

Dispositif de test multipuits Download PDF

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Publication number
WO2008129072A1
WO2008129072A1 PCT/EP2008/054979 EP2008054979W WO2008129072A1 WO 2008129072 A1 WO2008129072 A1 WO 2008129072A1 EP 2008054979 W EP2008054979 W EP 2008054979W WO 2008129072 A1 WO2008129072 A1 WO 2008129072A1
Authority
WO
WIPO (PCT)
Prior art keywords
filter material
pressure plate
filter device
filter
retentate
Prior art date
Application number
PCT/EP2008/054979
Other languages
German (de)
English (en)
Inventor
Timothy Sharbel
Uwe Blumenthal
Original Assignee
Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung filed Critical Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung
Priority to EP08749693A priority Critical patent/EP2142299B1/fr
Publication of WO2008129072A1 publication Critical patent/WO2008129072A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates

Definitions

  • the present invention relates to a filter device with which a plurality of samples can be filtered without the need for a vacuum or overpressure, with which the filtrate is drawn through the filter material. Rather, the filter device according to the invention operates only with gravity, optionally enhanced by an acceleration in the direction of Filtrathnes through the filter material, i. perpendicular to the filter material, for example generated by centrifugal force upon rotation of the device about an axis.
  • the filter device according to the invention provides a plurality of wells which are sealed on one side with filter material to filter a plurality of liquid samples offset in time or in parallel, for example in microtiter plate format with 96 or 384 parallel arranged wells. According to the invention, it is provided that the cups are arranged in one or more rows and their housing walls are connected to each other.
  • US 2004/0149659 A1 describes a filter device in the microtiter plate format, in which the bottom of a cup is covered by a grid and has an outlet opening. Above the grid is a filter, which is held by a sealing ring against the grid. The sealing ring is clamped to fix its position in the cup in its inner surface.
  • US 5861094 describes a two-part filter for filtering a single sample in which an annular shoulder is inclined within a tube at an angle to the axis of the tube and on this shoulder a filter material is arranged from a disposed in the first tube second coaxial Smaller diameter tube is pressed against the shoulder. The filter material is clamped at an angle to the common axis of the two tubes.
  • EP 1366819 A1 describes a microtiter plate whose well bottoms are formed by a filtration membrane for filtration under centrifugal force and / or under pressure.
  • the filtration membrane is supported by webs located below, which stand on a funnel located below the membrane, which covers the cross section of a bowl and has a central outlet opening.
  • the alternative filter device should have a simpler structure than known filter devices.
  • the filter device and the method should preferably be functional without connected source for positive or negative pressure, or be suitable for use without applied positive or negative pressure, but only below Gravity influence can be used, optionally reinforced by the action of acceleration forces.
  • the invention achieves this object by providing a filter device in which a plurality of wells, into which the liquid sample to be filtered can be entered, hereinafter referred to as retentate cups, are covered on one side by a filter or sieve material.
  • the filter material for example a woven or non-woven fabric, or a membrane material with pores, which is preferably elastic, is preferably integral with the plurality of retentate cups interconnected by their walls, portions of the filter material covering the retentate cups on one side.
  • the filter material has at least one side integrally formed first sockets, which are integrally formed or glued on a first side of the carrier material and sealing against the Retentatnäpfe can be arranged to seal the covered by the nozzle portion of the filter material against the end surfaces of the retentate.
  • filter pieces are arranged on the filter material, e.g. molded or glued, which form the Retentatnäpfe.
  • the filter material (10) does not have a first nozzle (11) which can be arranged as a seal against the retentate cups, but nozzles arranged on the filter material are arranged around the open cross-sections (4) of the retentate cups and form the retentate cups.
  • the second nozzle arranged opposite the second nozzle can be arranged against a pressure plate, which optionally has seals adjacent to the second nozzle.
  • the filter device therefore provides for the subdivision of the filter material into sections through the nozzles arranged at least on one or both sides of the filter material, which are respectively to be arranged suitably to the end faces of the side walls of the retentate cups, or at least on one side on the filter material as first studs molded or glued Retentate cups before. Therefore, the first sockets, which are arranged on the first side of the filter material, produce a seal between liquid retentate cups and filter material, as well as a sealing delimitation of the portions of the filter material Filter material against each other, cover the individual Retentatnäpfe.
  • the fluid-tight subdivision of the filter material between the retentate cups is established by the molded-on or glued-on retentate cups.
  • the on the retentate cups opposite the second side of the filter material arranged second nozzle also serve to prevent mixing of liquid, namely on the filtrate side.
  • the second sockets are arranged opposite the first sockets on the filter material and can be fixed in the same manner at this.
  • the sealing arrangement of the first nozzle which are arranged on the first side of the filter material against Retentatnäpfe, or against the faces of their walls, which comprise the clear cross section of the Retentatnäpfe, by sealing arrangement or by pressing the filter material according to the invention against the plurality of suitably aligned Retentatnäpfe be achieved, or by fixing the filter material to the Retentatnäpfen or by integral formation of the filter material with the Retentatnäpfen.
  • the walls of the retentate cups form the first nozzle, which are arranged on one side on the filter material; the second nozzles arranged on the opposite side of the filter material are arranged on the filter material and may e.g. Be openings of the pressure plate.
  • the nozzle and the Retentatnäpfe can be connected, for example, by gluing or melting or molding with the filter material.
  • a support grid which spans the cross section of the retentate cups and bears against the filter material on one side, is not required according to the invention; likewise, support webs which unilaterally support the filter material against pressure from the retentate cup are not necessarily present. Accordingly, the filter device according to the invention can be designed without parallel to the filter material arranged support grid or support webs, as is preferred.
  • the filtration method according to the invention which can be carried out only under gravity or without applied additional pressure gradient, is based on the fact that a contact pin, preferably at least one, particularly preferably exactly one, covers the first socket or retentate cups contacted opposite the second surface of the filter material.
  • the contact pin is approximately perpendicular to the second surface of the filter material and disposed approximately centrally within the portion of the filter material bounded by first and / or second sockets.
  • the contact pin is fixed by at least one connecting piece, preferably exactly one, two or three symmetrically arranged around the contact pin connecting pieces on the second piece.
  • the connector (s) preferably extend radially or pin-shaped from the contact pin and more preferably have a cross-sectional area perpendicular to the axis of the contact pin which has a maximum diameter of the contact pin width over the contact pin-to-second distance Cross-sectional area in the clear cross-section of the second nozzle to form.
  • a connecting piece may preferably extend radially from the contact pin to the second neck with a thickness which is measured in a plane perpendicular to the longitudinal axis of the contact pin and at most the diameter of the contact pin.
  • the length of the connector parallel to the axis of the contact pin may be smaller, preferably 10 to 50% larger, than the length of the connector adjacent to the second nozzle adjacent to the contact pin to permit contact of liquid moving along the contact pin avoid the second neck.
  • the device of the filter material according to the invention with one or both sides matching the end faces of the Retentatnäpfe arranged nozzles adjacent to the Retentatnäpfe or with nozzles forming Retentatnäpfe, wherein the filter material on the surface opposite the Retentatnäpfen surface from a first end of a contact pin is contacted, which is in communication with a located at the opposite end of the first end of the second pin of the contact pin and the guide pin is introduced into the volume of a Filtratnapfs.
  • a pressure plate is preferably arranged against the second nozzle.
  • the carrier When providing a pressure plate, the carrier can be fixed to hold the contact pin on the inner wall of a congruent with the first and / or second nozzle can be arranged recess of the pressure plate. More preferably, aligned to the filter material or the Retentatnäpfen aligned Filtratnäpfen below the Retentatnäpfe, wherein the guide pins protrude into the spanned by the walls of the Filtratnäpfe inner volume of Filtratnäpfe.
  • a pressure plate if present, rests with areas on the filter material against which the first sockets are arranged, or on the second sockets, in order to put the first sockets under tension against the end faces of the retentate cups.
  • the pressure plate has openings which are arranged to match the portions of the filter material bounded by the first neck in order to allow filtrate to pass from the filter material into filtrate cups.
  • at least one vent opening is arranged to allow air to escape from the Filtratnapf, which is displaced by entering filtrate.
  • the ventilation opening can be produced, for example, by a spacing of the filtrate cup and the second connection piece, wherein the spacing is produced, for example, by spacers between the pressure plate and the arrangement of filtrate cups.
  • the filter material has no first and / or second ports, and the open cross-sections of the retentate cups are surrounded by elastic sealing material so that when the filter material is placed against the sealing material at the retentate cups, a fluid seal is formed around the portions of the filter material. which adjoin the Retentatnäpfe.
  • the pressure plate preferably has seals which protrude beyond a first surface of the pressure plate which faces the filter material and comprise the openings of the pressure plate, particularly preferably when the filter material has only first nozzles and no second nozzles, so that the filter material directly contacted the printing plate.
  • These seals of the pressure plate can be arranged against the filter material and pressed and serve to prevent the passage of filtrate between adjacent portions of the filter material.
  • the seals of the pressure plate may be formed integrally with the pressure plate or placed on the pressure plate, e.g. glued or in-grooves, which comprise the openings of the pressure plate, be arranged elastic seals, e.g. O-rings.
  • the pressure plate has at least one contact pin within its openings, the first end of which protrudes at least into the plane in which the surface of the pressure plate lies, or which is stretched by seals of the pressure plate.
  • the contact pin of each opening of the pressure plate is arranged with the pressure plate can be arranged against the filter material or against the second nozzle of the filter material. Therefore, the first end of a contact pin contacts the portion of the filter material encompassed by the first neck or is located at a small distance therefrom. As a result, filtrate, after passing through the filter material, may flow along the contact pin, reducing or preventing distribution of the filtrate across the surface of the opening in the pressure plate.
  • the contact pins cause the passage of filtrate through a filter material when the filter material is a membrane with pores of nominal diameter of about 10 to 0.45 microns, without requiring a pressure difference across the filter material.
  • the present invention allows the passage of filtrate through so-called ultrafiltration membranes, which have hitherto been substantially impermeable to the filtrate without an applied pressure gradient.
  • the filter device according to the invention can also be used when used with filter material with nominal pores of 10 to 0.2 .mu.m, preferably from 2 to 0.45 .mu.m without a pressure gradient across the filter material or without connection for negative pressure and / or or overpressure, as is provided in the prior art for ultrafiltration.
  • each contact pin opposite the first end of a contact pin can protrude into the plane in which the second surface of the pressure plate lies, opposite its seals or opposite the retentate cups.
  • the second ends of contact pins preferably protrude beyond the second surface of the pressure plate and in each case form at least one guide pin along which filtrate can flow.
  • Such guide pins preferably protrude so far beyond the second surface of the printing plate that they span a distance, for example, made by spacers on the second surface of the printing plate to each opening of Filtratnäpfen, and more preferably protrude into spaced apart from the pressure plate Filtratnäpfe.
  • the guide pins span the spacing of Filtratnäpfen to second nozzle or to the pressure plate, wherein the spacing forms the ventilation opening for the escape of displaced air from the Filtratnäpfen.
  • Guide pins may each be locatable in the inner volume of the filtrate cups without contacting the inner surface of the filtrate cups, or the guide pins may, preferably when elastically deformable, contact the inner surface of the filtrate cups when spaced or immediately adjacent to the pressure plate or the Retentatnäpfen are arranged.
  • guide and contact pins can each be separated from one another in the opening of the pressure plate be defined space, each connected to one or more individual or common connectors within the opening with the pressure plate.
  • Contact pins and guide pins can be formed integrally with the pressure plate.
  • contact pins and / or guide pins are connected within an opening of the pressure plate by means of at least one connecting piece with the pressure plate.
  • contact pins and guide pins are provided with a hydrophobic surface, consist for example of hydrophobic material and / or are coated with hydrophobic material, e.g. with a hydrophobic polymer, e.g. Polytetrafluoroethylene, or with lipid, saturated olefin or wax.
  • Hydrophobic contact and guide pins enhance the passage of filtrate through the filter material into the filtrate wells, particularly retentate and filtrate wells or small diameter, small diameter, e.g. in the range of 1 to 3 mm, in particular 1, 5 to 2mm.
  • a large number of appropriately aligned collecting or filtrate cups are arranged to collect the filtrate, for example in the form of a microtiter plate.
  • the filtrate cups are open on one side, with their openings being arranged parallel to the filter material, in each case suitably aligned with the sections defined by the nozzles.
  • the filter material may have mesh sizes in the range of 0.22 to 200 ⁇ m, preferably 45 to 150 ⁇ m, and e.g. of polymers, polyethylene, polypropylene, polycarbonate, mixtures and copolymers thereof or of metal.
  • the nozzle according to the invention which are arranged on one or both sides of the filter material, may be formed on one or both sides by an elastic material is formed on the filter material or adhered, which has a substantially constant thickness and openings whose edges form the nozzle.
  • a particular advantage of the device according to the invention lies in the large number of wells in which a large number of samples can be filtered in parallel and isolated from each other without overpressure or underpressure, and in their simple structure, which is limited to a few components. Furthermore, the structure of the filter device enables easy disassembly, cleaning of the retentate cups and the filter material and reassembly so that multiple use is possible. In particular, in the preferred embodiment with contact pins, more preferably with additional guide pins, the device reduces or prevents the mixing of samples located in adjacent retentate and filtrate wells.
  • FIG. 1 shows a schematic cross-sectional view of an embodiment of the invention
  • FIGS. 2A and 2B are schematic cross-sectional views of two embodiments of the invention.
  • FIG. 3 shows a plan view of the sectional view of FIG. 2
  • Figure 4 is a schematic exploded view of an embodiment of
  • Figure 5 schematically an integral embodiment of retentate cup
  • Filter material for use in the device according to the invention shows
  • Figure 6A shows a schematic cross-sectional view of a portion of a printing plate
  • FIG. 6B shows a schematic plan view of a portion of FIG. 6A and FIG. 6B
  • Figure 7 schematically shows a sectional view of a preferred embodiment of
  • FIG. 1 schematically shows a plurality of retentate cups 1, which are arranged parallel next to each other, whose walls 2 adjoin one another, or are formed by a common section.
  • the Retentatnäpfe 1 have an open end 3, in which the liquid to be filtered can be entered, and an opposite cross-section 4, which is disposed adjacent to the filter material 10.
  • the filter material 10 has on its first side first port 11 and on its second, opposite side second port 12, which are each arranged and aligned to match the end faces 5 of the walls of the retentate cups 1.
  • the first pipe 11 and the second pipe 12 define between them suitably arranged portions 13 of the filter material 10, each covering at least a portion of the cross sections 4 of the retentate cups 1.
  • the first sockets 11 of the filter material 10 sealingly adjoin the end faces 5 of the walls of the retentate cups 1, so that liquid, which is discharged through the open end 3, under gravity or with additional acceleration through the portions 13 of the filter material 10 between the first nozzle 11th or the second nozzle
  • the retentate cups 1 and the filtrate cups 20 have the same dimensions, for example corresponding to a conventional microtiter plate with 96 or more cups arranged parallel to one another.
  • the filtrate cups 20 are arranged at a distance from the second nozzle 12, so that a distance is set between the second nozzle 12 and the walls of the collecting cups 20, which can serve as a ventilation opening 30.
  • the pressure plate 40 on contact pieces 46 which are formed within the openings 41 contact pins 44 on.
  • These contact pins 44 protrude at least into the plane of the openings 41, which is spanned by seals 43.
  • the contact pins 44 may extend to the filter material.
  • the seals 43 are arranged peripherally closed around the openings 41 on the side of the pressure plate 40 which faces the filter material 10 and are arranged in the region which can be arranged opposite the end faces of the walls 2 of the retentate cups 1 or in the region , the opposite to the first port 11 and / or to the second port 12 can be arranged.
  • the seals to the first and / or second ports 11, 12 are arranged suitably aligned to be disposed against the filter material 10 and the second nozzle 12, respectively.
  • a flow path is generated for each retentate cup 1, which is open only to the filtrate cup, whose cross section is spanned by the filter material 10.
  • a flow path is set, which is spaced from the walls of the opening 41 of the pressure plate 40, which additionally contributes to reducing a passage of filtrate in the not properly aligned to a retentate cup filtrate.
  • Figure 2A schematically shows a cross-sectional view through the filter material 10 and shows the first port 11 and the second port 12 in the form of portions of an elastic material, such as a plastic polymer, which is applied on both sides of the filter material 10 and has recesses, the edges of which first port 11 and the second port 12 form.
  • an elastic material such as a plastic polymer
  • FIG. 2B has no second connecting pieces 12 on the second side of the filter material 10.
  • FIG. 3 schematically shows a plan view of the filter material 10 shown in FIG. 2, the sections 13 of which are formed by the elastic material having recesses in these regions.
  • the elastic material disposed adjacent to the portions 13 of the filter material 10 forms the first sockets 11 on the first side of the filter material 10 and the second sockets 12 on the second side of the filter material 10, respectively.
  • the sealing arrangement of the first nozzle 11 against the end faces 5 of the walls of the retentate cups 1 can be effected in that a pressure plate 40 is arranged under tension against the second nozzle 12.
  • a pressure plate 40 has openings 41 in the areas corresponding to at least a portion of the portions 13 of the filter material 10 and are aligned with the portions 13 of the filter material 10.
  • the ventilation openings 30 can be provided as at least partial spacing between the pressure plate 40 and the filtrate or collecting cups 20.
  • first port 11 and second port 12 of the filter material 10 can be done by arranging the Retentatnäpfe 11 and the pressure plate 40 between parallel spaced plates which are interconnected.
  • FIG. 4 Such an arrangement of the filter device according to the invention is shown schematically in Figure 4 as an exploded view.
  • the retentate cups 1 are arranged in a plurality of parallel rows with their open ends 3 and their cross-sections 4 in each case in a parallel spaced-apart plane.
  • the filter material 10 is arranged between elastic material applied on both sides, which respectively forms the first connection 11 and second connection 12, which delimit sections 13 of the filter material 10 between them.
  • the sections 13 of the filter material 10 are to be arranged congruently with the cross sections 4 of the retentate cups 1 and extend over at least part of the cross sections 4, preferably completely over the cross sections 4.
  • the pressure plate 40, filter material 10, and retentate cups 1 are between parallel spaced rails 51, 52 arranged, for example may be provided as an integral part of a clamping device 50.
  • the arrangement of Retentatnäpfen 1, pressure plate 40 with arranged between them filter material 10, the first and second ports 11, 12 are respectively disposed against the pressure plate 40 and against the end faces 5 of the retentate cups, inserted between the rails 51, 52.
  • the pressure plate 40 against the second port 12 of the filter material 10 under tension, that the pressure plate 40 is clamped against the retentate cups 1, for example by fasteners (not shown), eg screws, pulling the Retentatnäpfe 1 against the pressure plate 40. Holes 6 for such screws are connected to the Retentatnäpfen 1, holes 42 with the pressure plate 40.
  • One of the pairs in pairs aligned bores 42 of the pressure plate 40 and the bores 6 of the Retentatnäpfe 1 are preferably provided with a thread.
  • the filter material 10 preferably also has holes 14 for the passage of fasteners, which serve to align the filter material 10 between the Retentatnäpfen 1 and the pressure plate 40.
  • FIG. 6A shows a cross-section through a pressure plate 40 with seals 43 arranged around the openings 41 which, according to the preferred embodiment, protrude beyond the surface of the pressure plate 40, which can be arranged facing the filter material 10, in FIG. 6A the upper side.
  • the seals 43 are optional and may be made of the material of the printing plate, e.g. be formed, or made of a different material. Seals 43 may be made of non-elastically deformable material, e.g. made of PE, PP, PC, polyacrylate, in particular in embodiments in which the filter material 10 has second stubs 12 made of elastic material, which can be brought into contact with the seals 43. Seals 43 of adjacent openings 41 may be formed by a common structural element.
  • a respective contact pin 44 is arranged on one or two connecting pieces 45.
  • the connecting pieces 45 are each fixed to the inner wall of the opening 41 of the pressure plate 40, preferably integrally formed from the material of the pressure plate 40.
  • the contact pins 44 are shown in various embodiments, in which they protrude, for example, to the level in which the seals 43 protrude, or beyond the plane in which the seals 43 protrude.
  • the length of the contact pins 44 depends on the design of the filter material, and in the case of a filter material without a second connecting piece 12, preferably up to the plane into which the seals 43 project.
  • the contact pins 44 preferably extend around the extension of the second connecting pieces 12 above the plane into which the seals 43 extend. In this way, depending on the presence of the second nozzle 12, a contact of the contact pins 44 with the filter material 10 in the region of its portions 13, each extending over a cross section of the retentate 1, with arrangement of the pressure plate 40 parallel to the filter material 10 possible.
  • the guide pins 45 serve to guide the filtrate into the filtrate cups 20 to minimize passage into an adjacent filtrate cup 20. As shown in Figure 6A, guide pins 45 may project into the plane of the surface of the pressure plate 40 opposite the filter material.
  • the guide pins 45 protrude beyond the surface of the printing plate so that they project into adjacently disposed filtrate cups. In this way, the passage of filtrate through aerosols, which can form when falling of a drop from the contact pin 44 and Leitchan 45, reduced in adjacent Filtratnäpfe.
  • FIG. 6B shows a preferred embodiment, namely the one-piece design of contact pin 44 with guide pin 45, which are fixed by a common connection piece 46 on the inner wall of the opening 41 of the pressure plate 40.
  • FIG. 7 shows retentate cups 1, which are formed by walls 2 and span the cross section 4 of the retentate cups 1.
  • the cross sections 4 are covered by filter material 10.
  • the pressure plate 40 has in each case a contact pin 44 within an opening 41, wherein the opening 41 covers the cross section 4, is aligned with this and is interrupted by the contact pin 44, possibly also by guide pin 45.
  • the contact pin 44 and integrally formed therewith according to the preferred embodiment of this guide pin 45 are supported by 2 plate-shaped connecting pieces 46, which are respectively fixed to the inner wall of an opening 41 of the pressure plate 40, preferably connected thereto.
  • the connecting pieces 46 in this illustration preferably have a thickness in the sectional plane, i. in a plane perpendicular to the axis of the contact pin 44, equal to or less than the diameter of the contact pin 44.
  • the contact pin 44 and guide pin 45 are preferably of round cross-section.
  • Seals 43 may be formed as a projection and / or recess, which engage with the end face of the wall 2 of the retentate 1, optionally as shown with at least a portion of the filter material 10, which lies on the end face of the wall 2 of the retentate 1.
  • the contact pin 44 may contact the filter material 10.
  • the guide pin is so long that it projects through the open end 21 of the filtrate cup into the volume spanned by the filtrate cup 20.
  • the filtrate cups are at least partially spaced from the pressure plate, for example by spacers between the second nozzle or pressure plate and Filtratnäpfen or recesses of the wall of the filtrate and / or the second nozzle or the Pressure plate in order to escape from the Filtratnäpfen displaced medium, eg air, by entering filtrate.
  • the retentate cups 1 can be made by positive or non-positive connection, e.g. be connected by molding or gluing their walls 1 with the openings 41 surrounding portions of the pressure plate 40, so that the inventive filter device, the retentate 1, a one-sided final filter material 10, a filter material 10 contacting the contact pin 44 in an opening 41 a Pressure plate is arranged, and the pressure plate 40 is made.

Abstract

L'invention concerne un dispositif de filtration comprenant une pluralité de cupules de rétentat qui sont disposées au moins en une rangée, présentant des extrémités ouvertes et, dans un plan commun distant, des extrémités opposées avec des sections transversales ouvertes, et qui sont recouvertes d'un matériau filtrant. Le matériau filtrant présente, sur son premier côté, des premiers raccords façonnés qui délimitent des sections du matériau filtrant orientées de manière à s'adapter aux sections transversales ouvertes des cupules de rétentat, les premiers raccords (11) pouvant être disposés de manière hermétique contre les faces frontales des parois des cupules de rétentat. Le dispositif de filtration comprend également une pluralité de joints d'étanchéité qui entourent des ouvertures d'une plaque de pression disposées de manière adaptée aux sections transversales ouvertes des cupules de rétentat, et qui peuvent être disposés contre le matériau filtrant sous contrainte au moins à la périphérie des sections du matériau filtrant. La plaque de pression présente dans chaque ouverture, au moins une broche de contact, qui pénètre au moins jusque dans le plan de la surface, tournée vers le matériau filtrant, de la plaque de pression.
PCT/EP2008/054979 2007-04-24 2008-04-24 Dispositif de test multipuits WO2008129072A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP08749693A EP2142299B1 (fr) 2007-04-24 2008-04-24 Dispositif de test multipuits

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE202007005989U DE202007005989U1 (de) 2007-04-24 2007-04-24 Filterplatte
DE202007005989.2 2007-04-24

Publications (1)

Publication Number Publication Date
WO2008129072A1 true WO2008129072A1 (fr) 2008-10-30

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PCT/EP2008/054979 WO2008129072A1 (fr) 2007-04-24 2008-04-24 Dispositif de test multipuits

Country Status (3)

Country Link
EP (1) EP2142299B1 (fr)
DE (1) DE202007005989U1 (fr)
WO (1) WO2008129072A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
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US4427415A (en) * 1979-01-05 1984-01-24 Cleveland Patrick H Manifold vacuum biochemical test method and device
EP0596482A1 (fr) * 1992-11-04 1994-05-11 Millipore Corporation Appareil d'essais à cavités multiples
US5861094A (en) 1997-04-17 1999-01-19 Goehde; Wolfgang Filter for biological particle separation
US20020041834A1 (en) 1996-05-06 2002-04-11 Benny Ballin Method and device for handling specimens
EP1366819A1 (fr) 2002-05-23 2003-12-03 Millipore Corporation Plaque de filtration monobloc
US20040149659A1 (en) 2001-05-31 2004-08-05 Jeffrey Kane Well for processing a fluid

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EP1366819A1 (fr) 2002-05-23 2003-12-03 Millipore Corporation Plaque de filtration monobloc

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EP2142299B1 (fr) 2012-10-10
DE202007005989U1 (de) 2007-08-02

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