WO2008105509A1 - 選抜工程を経ないアグロバクテリウム菌による形質転換植物の作成方法 - Google Patents

選抜工程を経ないアグロバクテリウム菌による形質転換植物の作成方法 Download PDF

Info

Publication number
WO2008105509A1
WO2008105509A1 PCT/JP2008/053559 JP2008053559W WO2008105509A1 WO 2008105509 A1 WO2008105509 A1 WO 2008105509A1 JP 2008053559 W JP2008053559 W JP 2008053559W WO 2008105509 A1 WO2008105509 A1 WO 2008105509A1
Authority
WO
WIPO (PCT)
Prior art keywords
agrobacterium
differentiation
culturing
medium containing
transformed plant
Prior art date
Application number
PCT/JP2008/053559
Other languages
English (en)
French (fr)
Inventor
Yuji Ishida
Yukoh Hiei
Original Assignee
Japan Tobacco Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Tobacco Inc. filed Critical Japan Tobacco Inc.
Priority to EP08721008.4A priority Critical patent/EP2127517B1/en
Priority to JP2009501306A priority patent/JPWO2008105509A1/ja
Priority to US12/528,985 priority patent/US8058514B2/en
Publication of WO2008105509A1 publication Critical patent/WO2008105509A1/ja

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

　本発明は、新規のアグロバクテリウム菌による形質転換植物の作成方法を提供することを目的とする。 　本発明の形質転換方法は、アグロバクテリウム菌を接種した植物組織を、（ｉ）３，６－ジクロロ－ｏ－アニシン酸、４－アミノ－３，５，６－トリクロロピコリン酸および／または２，４，５－トリクロロフェノキシ酢酸を含む共存培地で培養する共存工程、および（ｉｉ）（ｉ）で得られた組織を選抜薬剤を含む再分化培地で培養し再分化させる再分化工程を含み、ここで、共存培養後の組織をオーキシンを含む培地で培養し形質転換体を薬剤選抜する選抜工程を、共存工程と再分化工程の間に含まないことを特徴の１つとする。
PCT/JP2008/053559 2007-02-28 2008-02-28 選抜工程を経ないアグロバクテリウム菌による形質転換植物の作成方法 WO2008105509A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP08721008.4A EP2127517B1 (en) 2007-02-28 2008-02-28 Agrobacterium-mediated method for producing transformed plant without selection step
JP2009501306A JPWO2008105509A1 (ja) 2007-02-28 2008-02-28 選抜工程を経ないアグロバクテリウム菌による形質転換植物の作成方法
US12/528,985 US8058514B2 (en) 2007-02-28 2008-02-28 Agrobacterium-mediated method for producing transformed plant without selection step

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-049172 2007-02-28
JP2007049172 2007-02-28

Publications (1)

Publication Number Publication Date
WO2008105509A1 true WO2008105509A1 (ja) 2008-09-04

Family

ID=39721331

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/053559 WO2008105509A1 (ja) 2007-02-28 2008-02-28 選抜工程を経ないアグロバクテリウム菌による形質転換植物の作成方法

Country Status (4)

Country Link
US (1) US8058514B2 (ja)
EP (1) EP2127517B1 (ja)
JP (1) JPWO2008105509A1 (ja)
WO (1) WO2008105509A1 (ja)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009122962A1 (ja) * 2008-03-31 2009-10-08 日本たばこ産業株式会社 アグロバクテリウム菌による形質転換植物の作成方法
JP2010502197A (ja) * 2006-08-31 2010-01-28 モンサント テクノロジー エルエルシー 選択を含まない植物形質転換
WO2012015039A1 (ja) * 2010-07-29 2012-02-02 日本たばこ産業株式会社 アグロバクテリウム菌を用いた、オオムギ属植物へ遺伝子導入を行う方法およびオオムギ属植物の形質転換植物の作成方法
WO2016104583A1 (ja) * 2014-12-24 2016-06-30 日本たばこ産業株式会社 アグロバクテリウム菌を用いた、ソルガム属植物への遺伝子導入方法およびソルガム属植物の形質転換植物の作成方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110162112A1 (en) * 2009-12-31 2011-06-30 Pioneer Hi-Bred International, Inc. Increasing time-efficiency of high-throughput transformation processes

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000023675A (ja) 1998-07-14 2000-01-25 Japan Tobacco Inc 広範な品種に適応可能なアグロバクテリウムによるトウモロコシの形質転換方法
JP2000342256A (ja) 1999-06-04 2000-12-12 Japan Tobacco Inc 植物細胞への遺伝子導入の効率を向上させる方法
JP2000342253A (ja) 1999-06-04 2000-12-12 Japan Tobacco Inc 植物細胞への遺伝子導入の効率を向上させる方法
JP2000342255A (ja) 1999-06-04 2000-12-12 Japan Tobacco Inc 植物細胞への遺伝子導入の効率を向上させる方法
WO2005017169A1 (ja) 2003-08-13 2005-02-24 Japan Tobacco Inc. 植物材料への遺伝子導入を行う方法
WO2005017152A1 (ja) 2003-08-13 2005-02-24 Japan Tobacco Inc. 銅イオンの添加により植物の形質転換効率を向上させる方法
WO2006122023A1 (en) 2005-05-09 2006-11-16 The Samuel Roberts Noble Foundation, Inc. Agrobacterium transformation of stolons

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2386125C (en) 2000-08-03 2010-07-13 Japan Tobacco Inc. Method of improving gene transfer efficiency into plant cells
EP1306441B1 (en) 2000-08-03 2008-12-17 Japan Tobacco Inc. Method of improving gene transfer efficiency into plant cells
EP1279737A1 (en) * 2001-07-27 2003-01-29 Coöperatieve Verkoop- en Productievereniging, van Aardappelmeel en Derivaten AVEBE B.A. Transformation method for obtaining marker-free plants
NZ537237A (en) * 2002-06-22 2008-01-31 Syngenta Participations Ag Method of transforming soybean using agrobacterium

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000023675A (ja) 1998-07-14 2000-01-25 Japan Tobacco Inc 広範な品種に適応可能なアグロバクテリウムによるトウモロコシの形質転換方法
JP2000342256A (ja) 1999-06-04 2000-12-12 Japan Tobacco Inc 植物細胞への遺伝子導入の効率を向上させる方法
JP2000342253A (ja) 1999-06-04 2000-12-12 Japan Tobacco Inc 植物細胞への遺伝子導入の効率を向上させる方法
JP2000342255A (ja) 1999-06-04 2000-12-12 Japan Tobacco Inc 植物細胞への遺伝子導入の効率を向上させる方法
WO2005017169A1 (ja) 2003-08-13 2005-02-24 Japan Tobacco Inc. 植物材料への遺伝子導入を行う方法
WO2005017152A1 (ja) 2003-08-13 2005-02-24 Japan Tobacco Inc. 銅イオンの添加により植物の形質転換効率を向上させる方法
WO2006122023A1 (en) 2005-05-09 2006-11-16 The Samuel Roberts Noble Foundation, Inc. Agrobacterium transformation of stolons

Non-Patent Citations (41)

* Cited by examiner, † Cited by third party
Title
AKULA C ET AL.: "BIOLOGIA PLANTARUM", vol. 42, 1 January 1999, KLUWER ACADEMIC PUBLISHERS, article "Improved Regeneration Efficiency from Mature Embryos of Barley Cultivars", pages: 505 - 513
CHAN, M-T.; CHANG, H-H.; HO, S-L.; TONG, W-F.; YU, S-M.: "Agrobacterium-mediated production of transgenic rice plants expressing a chimeric a-amylase promoter / P-glucuronidase gene.", PLANT MOL. BIOL, vol. 22, 1993, pages 491 - 506, XP009006601, DOI: doi:10.1007/BF00015978
CHENG, M.; FRY, J. E.; PANG, S.; ZHOU, H.; HIRONAKA, C. M.; DUNCAN, D. R.; CONNER, T. W.; WAN, Y.: "Genetic transformation of wheat mediated by Agrobacterium tumefaciens", PLANT PHYSIOL., vol. 115, 1997, pages 971 - 980, XP002918248
CHU, C.-C: "The N6 medium and its applications to anther culture of cereal crops", PROC. SYMP. PLANT TISSUE CULTURE. PEKING: SCIENCE, 1978, pages 43 - 50
CUI M-L ET AL.: "PLANT SCIENCE", vol. 166, 1 April 2004, ELSEVIER IRELAND LTD, article "A rapid Agrobacterium-mediated transformation of Antirrhinum majus L. by using direct shoot regeneration from hypocotyl explants", pages: 873 - 879
DE CLEENE, M.; DE LEY, J.: "The host range of crown gall.", BOT. REV., vol. 42, 1976, pages 389 - 466
DEJI, A.; SAKAKIBARA, H.; ISHIDA, Y.; YAMADA, S.; KOMARI, T.; KUBO, T.; SUGIYAMA, T.: "Genomic organization and transcriptional regulation of maize ZmRR1 and ZmRR2 encoding cytokinin-inducible response regulators", BIOCHIM. ET BIOPHYS. ACTA, vol. 1492, 2000, pages 216 - 220, XP004275678, DOI: doi:10.1016/S0167-4781(00)00076-2
FRAME B.R. ET AL.: "Improved Agrobacterium-mediated transformation of three maize inbred lines using MS salts", PLANT CELL REP., vol. 25, no. 10, 2006, pages 1024 - 1034, XP019423768, DOI: doi:10.1007/s00299-006-0145-2
FRAME B.R. ET AL.: "Improved Agrobasterium-mediated transformation of three maize inbred lines using MS salts", PLANT CELL REP., vol. 25, no. 10, 2006, pages 1024 - 1034, XP019423768 *
FRAME, B.R.; MCMURRAY, J.M.; FONGER, T.M.; MAIN, M.L.; TAYLOR, K.W.; TORNEY, F.J.; PAZ, M.M.; WANG, K.: "Improved Agrobacterium-mediated transformation of three maize inbred lines using MS salts.", PLANT CELL REP., vol. 25, 2006, pages 1024 - 1034, XP019423768, DOI: doi:10.1007/s00299-006-0145-2
FRAME, B.R.; SHOU, H.; CHIKWAMBA, R.K.; ZHANG, Z.; XIANG, C.; FONGER, T.M.; PEGG, S.E.K.; LI, B.; NETTLETON, D.S.; PEI, D.: "Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system.", PLANT PHYSIOL., vol. 129, 2002, pages 13 - 22, XP002263971, DOI: doi:10.1104/pp.000653
GOULD, J.; DEVEY, M.; HASEGAWA, 0.; ULIAN, E.C.; PETERSON, G.; SMITH, R.H.: "Transformation of Zea mays L. using Agrobacterium tumefaciens and shoot apex", PLANT PHYSIOL., vol. 95, 1991, pages 426 - 434
GRIMSLEY, N.; HORN, B.; RAMOS, C.; KADO, C.; ROGOWSKY, P.: "DNA transfer from Agrobacterium to Zea mays or Brassica by agroinfection is dependent on bacterial virulence functions.", MOL. GEN. GENET., vol. 217, 1989, pages 309 - 316
GRIMSLEY, N.; HORN, T.; DAVIS, J.W.; HORN, B.: "Agrobacterium-mediated delivery of infectious maize streak virus into maize plants", NATURE, vol. 325, 1987, pages 177 - 179, XP002982470, DOI: doi:10.1038/325177a0
GRIMSLEY, N.H.; RAMOS, C.; HEIN, T.; HORN, B.: "Meristematic tissues of maize plants are most susceptible to Agroinfection with maize streak virus.", BIO/TECHNOLOGY, vol. 6, 1988, pages 185 - 189
HIEI Y. ET AL.: "Improve protocols for transformation of indica rice mediated by Agrobacterium tumefaciens", PLANT CELL, TISSUE AND ORGAN CULTURE, vol. 85, no. 3, 2006, pages 271 - 283, XP019409331 *
HIEI, Y.; KOMARI, T.: "Improved protocols for transformation of indica rice mediated by Agrobacterium tumefaciens.", PLANT CELL, TISSUE AND ORGAN CULTURE, vol. 85, 2006, pages 271 - 283, XP019409331, DOI: doi:10.1007/s11240-005-9069-8
HIEI, Y.; OHTA, S.; KOMARI, T.; KUMASHIRO, T.: "Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA.", THE PLANT JOURNAL, vol. 6, 1994, pages 271 - 282
HUANG, X.; WEI, Z.: "Successful Agrobacterium-mediated genetic transformation of maize elite inbred lines.", PLANT CELL, TISSUE AND ORGAN CULTURE, vol. 83, 2005, pages 187 - 200, XP019268654
ISHIDA, Y.; SAITO, H.; HIEI, Y.; KOMARI, T.: "Improved protocol for transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens.", PLANT BIOTECHNOLOGY, vol. 20, 2003, pages 57 - 66, XP009070864
ISHIDA, Y.; SAITO, H.; OHTA, S.; HIEI, Y.; KOMARI, T.; KUMASHIRO, T.: "High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens.", NATURE BIOTECHNOLOGY, vol. 14, 1996, pages 745 - 750, XP002046364, DOI: doi:10.1038/nbt0696-745
KOMARI, T.; HIEI, Y.; SAITO, Y.; MURAI, N.; KUMASHIRO, T.: "Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers.", THE PLANT JOURNAL, vol. 10, 1996, pages 165 - 174
KOMARI, T.; SAITO, Y.; NAKAKIDO, F.; KUMASHIRO, T.: "Efficient selection of somatic hybrids in Nicotiana tabacum L. using a combination of drug-resistance merkers introduced by transformation.", THEOR. APPL. GENET., vol. 77, 1989, pages 547 - 552
LINSMAIER, E.; SKOOG, F.: "Organic growth factor requirements of tobacco tissue culture.", PHYSIOL. PLANT., vol. 18, 1965, pages 100 - 127, XP000617350
MITSURU NISHIGUCHI ET AL.: "JOURNAL OF FOREST RESEARCH", vol. 11, 1 June 2006, SPRINGER-VERLAG, article "An improved transformation system for Lombardy popular (Populus nigra var. italalica", pages: 175 - 180
MOONEY, P.A.; GOODWIN, P.B.; DENNIS, E.S.; LLEWELLYN, D.J.: "Agrobacterium tumefaciens-gene transfer into wheat tissues", PLANT CELL, TISSUES AND ORGAN CULTURE, vol. 25, 1991, pages 209 - 218, XP000560763
NEGROTTO, D.; JOLLEY, M.; BEER, S.; WENCK, A. R.; HANSEN, G.: "The use of phosphomannose-isomerase as a selection marker to recover transgenic maize plants (Zea mays L.) via Agrobacterium transformation", PLANT CELL REPORTS, vol. 19, 2000, pages 798 - 803
NOMURA, M.; KATAYAMA, K.; NISHIMURA, A.; ISHIDA, Y.; OHTA, S.; KOMARI, T.; MIYAO-TOKUTOMI, M.; TAJIMA, S.; MATSUOKA, M.: "The promoter of rbcS in a C3 plant (rice) directs organ-specific, light-dependent expression in a C4 plant (maize), but does not confer bundle sheath cell-specific expression.", PLANT MOL. BIOL., vol. 44, 2000, pages 99 - 106, XP002179458, DOI: doi:10.1023/A:1006461812053
NOMURA, M.; SENTOKU, N.; NISHIMURA, A.; LIN, J-H.; HONDA, C.; TANIGUCHI, M.; ISHIDA, Y.; OHTA, S.; KOMARI, T.; MIYAO-TOKUMORI, M.: "The evolution of C4 plants: acquisition of cis-regulatory sequences in the promoter of C4-type pyruvate, orthophosphate dikinase gene.", PLANT J., vol. 22, 2000, pages 211 - 221
POTRYCUS, I: "Gene transfer to cereals: an assessment.", BIO/TECHNOLOGY, vol. 8, 1990, pages 535 - 542
RAINERI, D.M.; BOTTINO, P.; GORDON, M.P.; NESTER, E.W.: "Agrobacterium-mediated transformation of rice (Oryza sativa L.", BIO/TECHNOLOGY, vol. 8, 1990, pages 33 - 38, XP000918272, DOI: doi:10.1038/nbt0190-33
SAMBROOK, J.; FRITSCH, E.F.; MANIATIS, T.: "Molecular Cloning: A Laboratory Manual, 2nd ed.,", 1989, COLD SPRING HARBOR LABORATORY PRESS
See also references of EP2127517A4
TANIGUCHI, M.; IZAWA, K.; KU, M. S. B.; LIN, J-H.; SAITO, H.; ISHIDA, Y.; OHTA, S.; KOMARI, T.; MATSUOKA, M.; SUGIYAMA, T.: "The promoter for the maize C4 pyruvate, orthophosphate dikinase gene directs cell- and tissue-specific transcription in transgenic maize plants.", PLANT CELL PHYSIOL., vol. 41, 2000, pages 42 - 48
TINGAY, S.; MCELROY, D.; KALLA, R.; FIEG, S.; WANG, M.; THORNTON, S.; BRETTELL, R.: "Agrobacterium tumefaciens-mediated barley transformation.", PLANT J., vol. 11, 1997, pages 1369 - 1376, XP009080096, DOI: doi:10.1046/j.1365-313X.1997.11061369.x
TOKI, S.; HARA, N.; ONO, K.; ONODERA, H.; TAGIRI, A.; OKA, S.; TANAKA, H.: "Early infection of scutellum tissue with Agrobacterium allows high-speed transformation of rice.", THE PLANT JOURNAL, vol. 47, 2006, pages 969 - 976, XP002479434, DOI: doi:10.1111/j.1365-313X.2006.02836.x
TRIFONOVA S. ET AL.: "Agrobacterium-mediated transgene delivery and integration into barley under a range of in vitro culture conditions", PLANT SCIENCE, vol. 161, no. 5, 2001, pages 871 - 880, XP002958562 *
WATSON, B.; CURRIER, T.C.; GORDON, M. P.; CHILTON, M.-D.; NESTER, E. W.: "Plasmid required for virulence of Agrobacterium tumefaciens.", J BACTERIOL, vol. 123, 1975, pages 255 - 264
ZHANG, W.; SUBBARAO, S.; ADDAE, P.; SHEN, A.; ARMSTRONG, C.; PESCHKE, V.; GILBERTSON, L.: "Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants.", THEOR. APPL. GENET., vol. 107, 2003, pages 1157 - 1168
ZHAO, Z.-Y.; CAI, T.; TAGLIANI, L.; MILLER, M.; WANG, N.; PENG, H.; RUDERT, M.; SCHOEDER, S.; HONDRED, D.; SELTZER, J.: "Agrobacterium-mediated sorghum transformation.", PLANT MOL. BIOL., vol. 44, 2000, pages 789 - 798, XP002533153, DOI: doi:10.1023/A:1026507517182
ZHAO, Z.-Y.; GU, W.; CAI, T.; TAGLIANI, L.; HONDRED, D.; BOND, D.; SCHROEDER, S.; RUDERT, M.; PIERCE, D.: "High throughput genetic transformation mediated by Agrobacterium tumefaciens in maize.", MOL. BREED., vol. 8, 2001, pages 323 - 333, XP001100034, DOI: doi:10.1023/A:1015243600325

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9617552B2 (en) 2006-08-31 2017-04-11 Monsanto Technology Llc Plant transformation without selection
JP2010502197A (ja) * 2006-08-31 2010-01-28 モンサント テクノロジー エルエルシー 選択を含まない植物形質転換
US10941407B2 (en) 2006-08-31 2021-03-09 Monsanto Technology Llc Plant transformation without selection
US10233455B2 (en) 2006-08-31 2019-03-19 Monsanto Technology Llc Plant transformation without selection
US8581035B2 (en) 2006-08-31 2013-11-12 Monsanto Technology Llc Plant transformation without selection
US8847009B2 (en) 2006-08-31 2014-09-30 Monsanto Technology Llc Plant transformation without selection
US8357836B2 (en) 2008-03-31 2013-01-22 Japan Tobacco Inc. Agrobacterium-mediated method for producing transformed maize or rice
AU2009232967B2 (en) * 2008-03-31 2015-04-02 Kaneka Corporation Agrobacterium-mediated method for producing transformed plant
WO2009122962A1 (ja) * 2008-03-31 2009-10-08 日本たばこ産業株式会社 アグロバクテリウム菌による形質転換植物の作成方法
US9284567B2 (en) 2010-07-29 2016-03-15 Japan Tobacco Inc. Method for gene introduction into hordeum plant using agrobacterium, and method for production of transformed plant of hordeum plant
WO2012015039A1 (ja) * 2010-07-29 2012-02-02 日本たばこ産業株式会社 アグロバクテリウム菌を用いた、オオムギ属植物へ遺伝子導入を行う方法およびオオムギ属植物の形質転換植物の作成方法
WO2016104583A1 (ja) * 2014-12-24 2016-06-30 日本たばこ産業株式会社 アグロバクテリウム菌を用いた、ソルガム属植物への遺伝子導入方法およびソルガム属植物の形質転換植物の作成方法
US10745706B2 (en) 2014-12-24 2020-08-18 Japan Tobacco Inc. Method for gene transfer into sorghum plant using Agrobacterium, and method for production of transgenic sorghum plant

Also Published As

Publication number Publication date
EP2127517B1 (en) 2014-12-17
EP2127517A1 (en) 2009-12-02
JPWO2008105509A1 (ja) 2010-06-03
US8058514B2 (en) 2011-11-15
US20100132066A1 (en) 2010-05-27
EP2127517A4 (en) 2010-06-30

Similar Documents

Publication Publication Date Title
CN106119322B (zh) 一种提高重组人源胶原蛋白生产水平的发酵工艺
WO2007110394A3 (de) Verfahren zur enzymatischen herstellung von 2-hydroxy-2-methylcarbonsäuren
WO2012054947A3 (de) Verfahren zur gewinnung von lignin
WO2008105509A1 (ja) 選抜工程を経ないアグロバクテリウム菌による形質転換植物の作成方法
WO2013036147A3 (en) A fermentation process
WO2005073390A3 (en) L-amino acid-producing microorganism and method for producing l-amino acid
EP2650370A3 (en) Improved carbon capture in fermentation
WO2009125924A3 (ko) 퓨트레신 고생성능을 가지는 변이 미생물 및 이를 이용한 퓨트레신의 제조방법
EP2749652A3 (en) A method for producing a target substance by fermentation
WO2012177619A3 (en) Microorganisms for producing 1,3-butanediol and methods related thereto
WO2006124779A3 (en) Methods for production of chitin and chitosan
MY146385A (en) Method for producing fermented milk using novel lactic acid bacteria
MX2009003555A (es) Metodo de cultivo para favorecer la produccion de vitamina k2 por bacterias lacticas y sus aplicaciones en la preparacion de productos alimenticios.
WO2011129462A3 (en) A YEAST EXTRACT CONTAINING γ-Glu-X OR γ-Glu-X-Gly AND A METHOD FOR PRODUCING THE SAME
GB0714671D0 (en) Microbial bioreaction process
WO2013112939A3 (en) Methods for succinate production
WO2008126667A3 (en) Method for producing lactic acid
MX2021010527A (es) Produccion fermentativa de carbohidratos por celulas microbianas que utilizan una materia prima mixta.
WO2009028338A1 (ja) 新規オキシダーゼ遺伝子、および該遺伝子を利用する３－インドールピルビン酸の製造方法
WO2007143117A3 (en) Differentiation of primate pluripotent cells to hepatocyte-lineage cells
WO2008093829A1 (ja) Ｌ－アミノ酸の製造法
MY159962A (en) Method for producing fermented milk
WO2009005123A1 (ja) インターフェロンβ産生促進剤及びその製造法
BR112013006883A2 (pt) processo de produção de ácido dicarboxílico
CN101701234B (zh) 一种用生物酶提取石榴皮渣中鞣花酸的方法

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08721008

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 2009501306

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 12528985

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2008721008

Country of ref document: EP