WO2008086750A1 - Procédé de fermentation mixte de callicarpa dichotoma (lour.) k. koch et d'un champignon comestible, produits préparés via le procédé et utilisations de ceux-ci - Google Patents

Procédé de fermentation mixte de callicarpa dichotoma (lour.) k. koch et d'un champignon comestible, produits préparés via le procédé et utilisations de ceux-ci Download PDF

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Publication number
WO2008086750A1
WO2008086750A1 PCT/CN2008/070069 CN2008070069W WO2008086750A1 WO 2008086750 A1 WO2008086750 A1 WO 2008086750A1 CN 2008070069 W CN2008070069 W CN 2008070069W WO 2008086750 A1 WO2008086750 A1 WO 2008086750A1
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WO
WIPO (PCT)
Prior art keywords
edible fungus
white scorpion
leaching solution
white
scorpion
Prior art date
Application number
PCT/CN2008/070069
Other languages
English (en)
Chinese (zh)
Inventor
Du Peng
Huixia Zou
Zhixian Xiong
Original Assignee
Du Peng
Huixia Zou
Zhixian Xiong
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Peng, Huixia Zou, Zhixian Xiong filed Critical Du Peng
Publication of WO2008086750A1 publication Critical patent/WO2008086750A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/062—Ascomycota
    • A61K36/066—Clavicipitaceae
    • A61K36/068—Cordyceps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/08—Drugs for disorders of the alimentary tract or the digestive system for nausea, cinetosis or vertigo; Antiemetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/14—Drugs for genital or sexual disorders; Contraceptives for lactation disorders, e.g. galactorrhoea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents

Definitions

  • the present invention relates to the field of food fermentation, in particular to a mixed fermentation method of white scorpion and edible fungi and a system for obtaining the same
  • White scorpion its different parts can treat different diseases, the effect is obvious: its skin, rhizome, oral leaching after boiling water, in the case of breast disease without ulceration, can quickly reduce inflammation, pain, swelling, usually After 24 hours, if the breast disease has ulcerated, its flower is smashed and applied externally. After 12 hours, hemostasis, pain relief, anti-inflammatory, and edema. The fleshy part of the root is boiled and drained by drinking water. Concussion and its sequelae are also effective. After oral medication, it can cure dizziness, headache and vomiting within 72 hours.
  • White scorpion is a kind of wild plant. Its resistance is very weak. When it encounters the residues of chemical products such as pesticides, chemical fertilizers and herbicides, it is easy to lose roots and die, so artificial planting is not easy to harvest. The output is very small, mostly for wild digging, but without limited artificial digging, limited resources will be exhausted.
  • the present invention provides a method for mixing and fermenting white scorpion and edible fungi, which can expand and utilize the white scorpion material and effectively protect the ecological environment.
  • the invention also provides articles obtained by the method and uses thereof.
  • a method for mixed fermentation of white scorpion and edible fungi characterized in that the white scorpion leaching solution is inactivated at room temperature and pressure after adding water and excipients, and then fermented with the fungal parent species.
  • the fermentation process comprises the following steps: (1) adding 20-25 volumes of water and excipients to the white scorpion leachate to boil, removing the juice from the slag, (2) adding the juice to the fermenter, inactivating the water vapor under normal pressure, cooling To 26 ° C ⁇ 2 ° C, (3) Inject the edible fungus into the fermenter and ferment it in depth.
  • the fermenter is a fermenter described in the utility model patent No. 03270560.3, entitled "Can lid and culture tank for edible fungus culture strainer”.
  • the edible fungus is injected into the syringe of the utility model patent No. 200320126960.X entitled "Edible bacteria liquid injection rod and syringe containing the injection rod”.
  • the deep fermentation time is 60-72 hours.
  • the effective solid content at the end of the deep fermentation is 70 ⁇ /1 ⁇ .
  • the white scorpion leachate is a leachate of white scorpion skin, stem and/or root, and the edible fungus is a black fungus parent.
  • the white scorpion leaching solution is a leaching solution of the white scorpion flower, and the edible fungus is a black fungus mother.
  • the white scorpion leaching solution is a leaching solution of the white scorpion root, and the edible fungus is a locust species.
  • the invention inactivates the white scorpion leachate by adding water and inactivates at normal temperature and normal pressure, and can completely retain the active ingredient of the white scorpion.
  • the inactivation device of normal temperature and normal pressure can adopt the "saturated steam pressureless boiler" invented by the inventor ( Patent No.: 200320126611.
  • the fermenter is directly pushed into the above boiler to inactivate, and then the edible fungus is injected into the fermenter for mixed fermentation, so that the white scorpion interacts with the polysaccharide in the edible fungus, so that the obtained product has The same effect of pure white scorpion leaching solution reduces the use of white scorpion plant resources, enabling the ecological environment to be effectively protected.
  • the fermentation time is preferably 60-72 hours, which can ensure sufficient fermentation, high effective solid content and high polysaccharide content, and the obtained product has the best therapeutic effect, and the fermentation is terminated with an effective solid content of 70 g/L or more.
  • Black fungus is rich in iron and has obvious hemostatic effect. Aphids are rich in various cordycepins, amino acids and polysaccharides, which can improve human immunity.
  • the black fungus or the Cordyceps militaris seed and the white scorpion leaching solution are mixed and fermented, and the fermentation material of the fungus is used to expand the raw material, and at the same time, the therapeutic effects of the above two edible fungi are combined to obtain a medicinal preparation.
  • the fermented liquid obtained by deep fermentation of the black fungus mother seed and the white scorpion skin, root and stem leaching solution is filtered, and the filter residue is dried to prepare a capsule. After the filtrate is inactivated, the liquid can be filled into an oral liquid or the filtrate is concentrated and crystallized to prepare a capsule. Oral solid preparations.
  • the preparation obtained by the above method can be used for oral administration of non-ulcerative breast disease, twice a day, and can rapidly reduce inflammation, stop swelling and edema within 24 hours, and the effect is remarkable.
  • the fermented liquid obtained by deep fermentation of the black fungus mother seed and the white scorpion flower leaching solution is filtered, and the filter residue is dried to prepare a powder. After the filtrate is inactivated, the crystal is concentrated and crystallized to prepare a powder.
  • the powder obtained by the above method can be used for external application of ulcerated breast disease, once a day, can stop bleeding, anti-inflammatory, analgesic, and edema within 12 hours.
  • the fermentation broth obtained by deep-fermenting the larva of the Cordyceps militaris and the succulent root of the white scorpion is filtered, and the slag is dried to prepare a capsule. After the filtrate is inactivated, it can be filled into an oral liquid or the filtrate is concentrated and crystallized to prepare an oral solid preparation. .
  • the preparation obtained by the above method can be used for oral administration of concussion and its sequelae, 3 times a day, and can cure dizziness, headache and vomiting within 72 hours, and the effect is obvious.
  • the various preparations prepared by the above fermentation method have the same medicinal effects as the pure white scorpion, effectively protecting the limited white scorpion plant resources, reducing the digging and mining, and revitalizing, adjusting and protecting the biological resources of the white scorpion.
  • the fungus of the black fungus using spore separation method, after incubating, plane culture, microscopic examination, stored at 4 ° C, and stored for no more than 90 days.
  • the slag juice is directly poured into a 200L fermenter.
  • the fermenter is preferably a fermenter, which is described in the utility model patent No. 03270650.3, entitled "Can lid and culture tank for edible fungus liquid culture tank”. It is necessary to leave 15% space, and inject a proper amount of defoamer or defoamer, lock the flange cover, push into the "saturated steam pressureless boiler" (patent number: 200320126611.
  • the concentration of concentrated sugar in the filtrate is 90%, and the purity of the polysaccharide in the crystal after concentration is 70%.
  • the therapeutic effect of the oral preparation obtained in Example 1 was as follows:
  • the effect of the 10 patients with breast disease after taking the drug of the present invention is as follows: All patients have clinical manifestations of double breast or single breast inflammation, redness, pain, and taking the drug of the present invention starts in about 24 hours. Detumescence, pain gradually reduced until normal, three days - five days can be cured, no effect is not obvious, the total effective rate reached 100%.
  • the slag juice is directly poured into a 200L fermenter.
  • the fermenter is preferably a fermenter described in the utility model patent entitled Patent No. 03270650.3, "Can lid and culture tank for edible fungus culture strainer".
  • Can lid and culture tank (patent number: 03270560.3) deep fermentation in 65 hours, after the concentration reaches the standard (refer to the sugar content), the tank is placed, filtered, and the solid is dried to make a powder for external use. The filtrate is inactivated. Concentration crystallization, powder crumbled into powder.
  • the concentration of concentrated sugar in the filtrate was 93%, and the purity of the polysaccharide in the crystal was 75% after concentration.
  • Example 2 The efficacy of the powder obtained in Example 2 was as follows: 5 cases of breast disease patients: The clinical manifestations of the patient were double breast or single breast inflammation, redness and ulceration. The topical application of the present invention began to detumescence after 12 hours, the erosion tissue was gradually reduced, the necrotic tissue was shed, and the newborn normal tissue was cured within five days to seven days. If the effect is not obvious, the total efficiency will reach 100%.
  • Example 3
  • the concentration of concentrated sugar in the filtrate is 95%, and the purity of the polysaccharide in the crystal after concentration is 80%.
  • Example 3 The oral preparations obtained in Example 3 were used as follows: 8 patients with concussion: The clinical manifestations of the patients were headache, dizziness, vomiting, and the symptoms of the drug were relieved after 72 hours, vomiting stopped, and recovered within five to seven days. If the effect is not obvious, the total efficiency will reach 100%. Typical Case:

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
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Abstract

L'invention concerne un procédé de fermentation mixte de Callicarpa dichotoma (Lour.) K. Koch et d'un champignon comestible tel qu'Auricularia auricula ou Cordyceps militaris (l. exfr) link, et les produits préparés via ledit procédé, le procédé comprenant les étapes d'ajout d'l'eau et d'un agent auxiliaire dans le lixiviat de Callicarpa dichotoma (Lour.) K. Koch, d'inactivation sous une pression et une température normales, puis de fermentation avec des souches mères de champignon comestible. Le procédé de fermentation peut utiliser Callicarpa dichotoma (Lour.) K. Koch efficacement et économiser la source de cette plante officinale, les produits préparés peuvent être utilisés, entre autres, pour le traitement d'une maladie de l'arbre galactophorique avec ou sans ulcération, d'une commotion cérébrale et de séquelles dues à celle-ci.
PCT/CN2008/070069 2007-01-12 2008-01-10 Procédé de fermentation mixte de callicarpa dichotoma (lour.) k. koch et d'un champignon comestible, produits préparés via le procédé et utilisations de ceux-ci WO2008086750A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CNB2007100626547A CN100494342C (zh) 2007-01-12 2007-01-12 白棠子与食用真菌的混合发酵方法、该方法获得的制品及其应用
CN200710062654.7 2007-01-12

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WO2008086750A1 true WO2008086750A1 (fr) 2008-07-24

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WO (1) WO2008086750A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112646726A (zh) * 2019-10-11 2021-04-13 孙傲 一种黑木耳发酵用培养基、发酵方法及发酵饮料

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100494342C (zh) * 2007-01-12 2009-06-03 彭都 白棠子与食用真菌的混合发酵方法、该方法获得的制品及其应用
CN102133283B (zh) * 2011-03-18 2012-11-14 彭都 一种治疗便秘的药物及其制备方法
CN103371367B (zh) * 2012-04-26 2015-01-07 彭都 艾滋病营养疗法的一种食品

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2659124Y (zh) * 2003-08-18 2004-12-01 彭都 食用菌液体菌种培养罐的罐盖及培养罐
CN2672466Y (zh) * 2003-12-08 2005-01-19 沈小茂 饱和蒸汽无压锅炉
CN2684548Y (zh) * 2003-12-16 2005-03-16 沈小茂 食用菌液体注射杆及包含该注射杆的注射器
CN1995323A (zh) * 2007-01-12 2007-07-11 彭都 白棠子与食用真菌的混合发酵方法、该方法获得的制品及其应用

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2659124Y (zh) * 2003-08-18 2004-12-01 彭都 食用菌液体菌种培养罐的罐盖及培养罐
CN2672466Y (zh) * 2003-12-08 2005-01-19 沈小茂 饱和蒸汽无压锅炉
CN2684548Y (zh) * 2003-12-16 2005-03-16 沈小茂 食用菌液体注射杆及包含该注射杆的注射器
CN1995323A (zh) * 2007-01-12 2007-07-11 彭都 白棠子与食用真菌的混合发酵方法、该方法获得的制品及其应用

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHENG W.: "Discrimination of medicinal materials of Callicarpa species", CHINESE TRADITIONAL AND HERBAL DRUGS, vol. 35, no. 8, August 2004 (2004-08-01), pages 947 - 951 *
LI Y. ET AL.: "Fermented TCM, to open a new field of TCM research & development", NATURAL PRODUCT RESEARCH AND DEVELOPMENT, vol. 16, no. 2, 2004, pages 179 - 181 *
YANG H. ET AL.: "The process of Chinese medicine by submerged fermentation of pharmaceutical fungi", CHINA JOURNAL OF CHINESE MATERIA MEDICA, vol. 30, no. 21, November 2005 (2005-11-01), pages 1717 - 1720 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112646726A (zh) * 2019-10-11 2021-04-13 孙傲 一种黑木耳发酵用培养基、发酵方法及发酵饮料

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CN100494342C (zh) 2009-06-03

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