WO2008013235A1 - Procédé de suppression de parasites sur la surface corporelle de poissons - Google Patents

Procédé de suppression de parasites sur la surface corporelle de poissons Download PDF

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Publication number
WO2008013235A1
WO2008013235A1 PCT/JP2007/064699 JP2007064699W WO2008013235A1 WO 2008013235 A1 WO2008013235 A1 WO 2008013235A1 JP 2007064699 W JP2007064699 W JP 2007064699W WO 2008013235 A1 WO2008013235 A1 WO 2008013235A1
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Prior art keywords
fish
feed
day
parasites
parasite
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PCT/JP2007/064699
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English (en)
Japanese (ja)
Inventor
Noritaka Hirazawa
Shinya Yamashita
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Nippon Suisan Kaisha, Ltd.
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Priority to JP2008526813A priority Critical patent/JPWO2008013235A1/ja
Publication of WO2008013235A1 publication Critical patent/WO2008013235A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/095—Sulfur, selenium, or tellurium compounds, e.g. thiols
    • A61K31/10—Sulfides; Sulfoxides; Sulfones
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
    • A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/111—Aromatic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • A61P33/14—Ectoparasiticides, e.g. scabicides

Definitions

  • the present invention relates to a method for controlling parasites of fish (particularly, cultured fish) and a fish culture feed for parasite control. More specifically, the present invention relates to a method and power for controlling body surface parasites such as Neo-Benedenia and Benedenia by oral administration of drugs, and a fish feed for controlling such parasites.
  • In-situ diagnostic methods include abdominal epidermis redness and fins, and the power of death with symptoms such as cloudiness of the eyeballs, and in fish that have received a large amount of parasitism, the body surface becomes cloudy due to massive secretion of mucus. Can be seen.
  • abnormal swimming such as rubbing the body against a ginger net, is often seen. By rubbing the body with ginger nets, the symptoms worsen, and the chance of infection by pathogenic bacteria from the parasitic sites increases, which may increase the damage. If parasitosis of this worm is confirmed, pay attention to the water temperature; it can be dewormed by performing a fresh water bath or hydrogen peroxide bath for about 3 minutes.
  • treatment with drugs that can be administered orally is strongly desired.
  • Kyokazu and Bayer Medical Co., Ltd. are used to combat the insects that have the common name Braziquantel (isoquinoline 'pyrazine derivative) parasitic on the surface of the perch fish It is sold by Fermentation Industries Co., Ltd. They are It is used by mixing with feed for farmed fish.
  • Braziquantel isoquinoline 'pyrazine derivative
  • Bithionol bis (2-hydroxy-3,5-dichlorophenyl) sulfide
  • Non-patent document 1 describes that bitonol is applied to hamachi under conditions of 300 mg / kg fish weight / day.
  • Non-patent document 1 confirms the anti-parasitic effect of bitionol, but it cannot be completely anthelmintic.
  • Non-patent document 1 describes and suggests whether parasites can be completely eradicated, the potential side effects, and dosages that completely eradicate and minimize side effects. Absent. In other words, Non-Patent Document 1 indicates that many problems still remain to completely eliminate the ectoparasite fluke (Benedenia) in fish, but does not teach a solution.
  • Non-Patent Document 1 Tsuguo Takada, Masagoro Kasahara (1965): “Study on the extermination of ectoparasites of hamachi”. Fishery breeding, 13, 23-27.
  • An object of the present invention is to provide a method for controlling body surface parasites in fish (particularly, cultured fish) with orally administered drugs.
  • the inventors have sought for an orally-administered drug effective against the common beetle, which is an important problem in the cultivation of apricots such as amberjack and yellowtail, and have already found various antiparasitic drugs for animals and substances derived from natural products. Explored. As a result, it was found that the effects of bithionoles marketed as veterinary antiparasitic drugs were found, and the present invention was completed.
  • the present invention is a method for controlling body surface parasites of fish according to (1) to (6).
  • a method for controlling body parasites of fish comprising orally administering 50-250 mg / kg body weight / day of bitonol to fish.
  • the fish is a flounder or perch fish (1)! /, (3) V, any body surface Parasite control method.
  • Fishes are amberjack, yellowtail, Japanese cypress, Japanese cypress, Japanese cedar, Japanese horse mackerel, sea bass, sea bass, red sea bream, yellowtail, Japanese pheasant, fly, Japanese flounder, sea bream, trough puffer fish, Japanese sea bream, Japanese sea bream, Japanese mackerel, bonito, turbot, tilapia Either (1) or (3) any method of controlling body surface parasites.
  • the present invention also relates to a fish feed for controlling body parasites according to (7) to (; 11).
  • a fish feed for controlling body parasites characterized in that bitionol is added so that fish can take 50-250 mg / kg body weight / day of bitonol.
  • bitonol is added to the feed for flounder fish so that the flounder fish can take 50-200 mg / kg body weight / day of bitonol feed.
  • FIG. 1 is a graph showing the anthelmintic effect of oral administration of bithionol on neobenedenia infested with flounder in Example 1 and the effect on feeding.
  • FIG. 2 is a graph showing the anthelmintic effect of oral administration of bithionol on neobenedenia infested with Japanese flounder and the effect on feeding in Example 2.
  • FIG. 3 is a graph showing the anthelmintic effect of oral administration of bithionol on neobenedenia infested with Japanese flounder in Example 2, using the total length of neobenedenia as an index.
  • FIG. 4 is a graph showing the anthelmintic effect of oral administration of bitonol on neobenedenia parasitized with amberjack and the effect on feeding in Example 3.
  • FIG. 5 is a graph showing the anthelmintic effect of oral administration of bitonol on neobenedenia parasitic on amberjack in Example 3, using the total length of neobenedenia as an index.
  • FIG. 6 is a graph showing the anthelmintic effect of oral administration of bitonol on neobenedenia parasitized with amberjack and the effect on feeding in Example 4. (Feeding;! ⁇ 3 days were 2% of fish weight, 4 days was 4% fish weight, and all were feedd with no drug).
  • FIG. 7 is a graph showing the anthelmintic effect of oral administration of bitonol on neobenedenia infested with amberjack and the effect on feeding in Example 5.
  • FIG. 8 is a graph showing the relationship between the body length of neobenedenia parasitized with amberjack and the dose required for the expression of anthelmintic effect in Example 6.
  • FIG. 9 is a graph showing the relationship between the length of neobenedenia parasitized with amberjack and the dose required for the expression of anthelmintic effect in Example 7.
  • FIG. 10 shows the anthelmintic effect of oral administration of bitionol on neobenedenia infested with amberjack (large fish) in Example 8.
  • FIG. 11 is a graph showing the time-dependent change in the number of parasites after oral administration of bitonol to neobenedenia parasitized with amberjack in Example 9.
  • FIG. 12 is a graph showing bites of neobenedenia parasitic on amberjack in Example 9. It is the photograph which showed the change of the worm body after oral administration of onol.
  • FIG. 13 is a graph showing the anthelmintic effect of oral administration of bitonol on neobenedenia parasitized with pri and the influence on feeding in Example 11.
  • FIG. 14 is a graph showing the anthelmintic effect of oral administration of bitonol on neobenedenia parasitized with pri and the effect on feeding in Example 12.
  • Bithionol used in the present invention is a compound represented by bis (2_hydroxy-3,5-dichlorophenyl) sulfide.
  • Drugs containing bitionol are sold under the trade names of Silnac Paste and Animal Vitin from Dainippon Pharmaceutical Co., Ltd. and Tanabe Seiyaku Co., Ltd. as cattle and horse antiparasitic drugs (for example, tapeworms).
  • the anti-parasitic effect of the bitonol of the present invention is a parasite that parasitizes the body surface of fish, generally called neobenedenia, benedenia, etc., which are called hadamushi, monoprostrate girodactinoles (salmonid fish, flounder, tiger puffer, goldfish, It is effective for parasitism mainly in guppy. It is particularly effective for Neo Benedenia and Benedenia, which are generally called Hadamushi.
  • the fishes that are the subject of the present invention are flounder and perch fishes that have such a beetle infested on the surface of the body, and are prey, flounder, puffer, and Thai fishes.
  • amberjack, yellowtail, sea bass, yellow hag, hiramasa, hinagacampa, cedar, maji, shimaji horse mackerel, red sea bream, yellowtail, flyfish, Japanese flounder, flounder, macka moth, sea bream, sea bream, bonito Examples include turbot and tilapia.
  • Bitionol when added to the feed and administered at higher doses, causes eating disorders and some drown. Therefore, it is important to use a dosage that is effective and free of side effects. As shown in the examples, bitonol clearly causes eating disorders in both flounder and amberjack at over 300 mg / kg / day. In particular, in Puri, eating disorders and moribundity are observed even at 200 mg / kg / day. From the examples, it is considered that the single oral dose at which drowning is observed in amberjack is in the range of 300 to 400 mg / kg / day, and in the range of 100 to 200 mg / kg / kg in pu and li. No effect is observed at 50 mg / kg / day or less.
  • Eating disorders and drowning which are side effects of vithonol, are observed immediately after administration, so the ability to be a side effect fish species can be determined by administering high and low doses to a small number of fish for 13 days. Judgment can be made.
  • the effect of vithonol can be obtained with a smaller dose as the fish size increases.
  • the dose to suppress the parasite almost completely decreased to 166 mg / kg / day for the 43 g size, 136 mg / kg / ⁇ for the 116 g size, 123 mg / kg / ⁇ for the 220 g size, and a little later. It has been confirmed that even the 480g size is completely suppressed at 144mg / kg / day. No side effects have been observed when 200 mg / kg / day is administered daily, so there is no major problem even if the same dose is administered, but a smaller dose may be used when administering to larger fish.
  • the flounder or perch fish feed is a feed that takes into account the nutritional components and physical properties required by flounder or perch fish. Usually mixed with fish meal, salmon, starch, minerals, vitamins, fish oil, etc. into pellets, or Pellets made by mixing frozen fish such as sea bream and powdered feed (mash) with vitamins added to fish meal are used. Depending on the type and size of the fish, the daily food intake is almost fixed, so the amount of bitionol converted to the above dosage is added to the feed.
  • Bitionol can be given in a single daily dose or in several divided doses. If there is a tendency to decrease food intake due to bitonol, it is possible to reduce the effects on food intake by giving it once rather than taking several times a day. For example, in the case of a fish that is fed once a day, for example, a daily dose of bithonol is added to a feed that is about 80% of the total feed, and the feed is first served to ensure that the required amount of bitonol is added. Ingest. In addition, in the case of fish fed multiple times a day, a daily dose of bithonol should be added to the first feed. Since bitionol is a stable substance with a melting point of 188 ° C, it can be used with the power S to add calories even in the production process of feed that has a heating process.
  • Hadamushi (Neobenedenia girellae) used in the following examples is maintained and maintained using Hoshigarai as a host! /, And eggs hatched from adults were collected, cultured, and hatched. Larvae were used for testing.
  • Test method 60 flounder with an average fish weight of about 78 g were raised in a 200 liter aquarium for about 7 days and adapted to a water temperature of 25 ° C. During that time, we used commercial feed and the feeding rate was 1.5% of fish weight. The water injection was 2.4 liters / minute. Parasitic infection was carried out by putting about 12,000 Neo Benedenian hatchling larvae into a 200-liter aquarium and exposing fish to the parasite for 1 hour. After infection, 10 fish were housed in 6 100-liter water tanks. Water injection during the breeding period was 1.2 liters / minute.
  • Test group 8 days after exposure to parasites, force, et al. 66 mg, 128 mg, 197 mg, 312 mg / kg fish body weight / day for 3 days
  • a total of 6 groups were established: a positive control group to receive Praziquantel (commercially available anti-spotted beetle agent) at 155 mg / kg fish weight / day, and a control group (Omg) to which no feed was added. .
  • a predetermined amount of each drug was added to the moist feed to obtain a test feed.
  • Test method Fifty flounder with an average fish weight of about 29 g were raised in a 200 liter aquarium for about 7 days and acclimated to a water temperature of 25 ° C. During that time, we used commercial feed and the feeding rate was 1.5% of fish weight. The water injection was 2.4 liters / minute. Parasitic infection was carried out by using a 200-liter aquarium as water and introducing approximately 10,000 neo-Benedenian hatchling larvae and exposing the parasite to fish for 1 hour. After infection, 10 fish were housed in five 100-liter water tanks.
  • Test area 8 days after exposure to parasite, etc. 6 mg, 18 mg, 52 mg, 66 mg / kg fish weight / day for 3 days A total of 5 groups were set, 4 groups with oral administration of intervitionol, and 4 control groups (Omg) with feed containing no drug. A predetermined amount of each drug was added to the moist feed to make a test feed.
  • the parasite length was 2.59 ⁇ 0.55 mm (average soil standard deviation) in the control group, 2.68 ⁇ 0.44 mm in the 6 mg / kg group, 2.41 ⁇ 0.54 mm in the 18 mg / kg group, and 2.30 ⁇ 0.42 in the 52 mg / kg group. mm, 66 mg / kg force 3 ⁇ 4 ⁇ 12 ⁇ 0.27 mm (Fig. 3).
  • Significant differences were observed in the 52 mg / kg group (p ⁇ 0.05) and 66 mg / kg group (p ⁇ 0.01) compared to the parasite length in the control group.
  • * is significantly different from the control group (Omg) in comparison with p ⁇ 0.05
  • ** is significantly different from p (0.01) in comparison with control group (Omg). I mean.
  • Test method 50 Amberjack with an average fish weight of about 24 g was raised in a 200 liter aquarium for about 7 days and acclimated to a water temperature of 25 ° C. During that time, we used commercial feed and the feeding rate was 4% of fish weight. The water injection was 2.4 liters / minute. Parasitic infection was achieved by using a 200-liter aquarium as water, introducing about 10,000 neo-Benedenian hatchling larvae, and exposing fish to the parasite for 1 hour. After infection, 10 fish were housed in five 100-liter water tanks.
  • Test group 8 days after exposure to parasites, 63 mg, 127 mg, 193 mg, 332 mg / kg fish body weight / day, orbital administration group for 4 days in which biotinol is administered orally, feed with no additives A total of 5 control groups (Omg) were set. A predetermined amount of each drug was added to the moist feed to make a test feed.
  • the parasite cannot be completely anthelmintic, and it has been found that the optimum dose of this product for yellowtail is higher than that of Japanese flounder.
  • the 193 mg / kg dose group almost completely abolished this parasite.
  • drowning occurred one day after power feeding that completely exterminate the parasite, and an extreme decrease in appetite was observed on the second day of feeding, with side effects.
  • the 193 mg / kg group a clear decrease in appetite was observed on the third day of administration, but since a complete anthelmintic effect was observed, single or double administration may be effective at this concentration. It was.
  • Test method 60 amberjacks with an average fish weight of about 26 g were bred in a 200 liter water tank for about 7 days and acclimated to a water temperature of 25 ° C. During that time, we used commercial feed and the feeding rate was 4% of fish weight. The water injection was 2.4 liters / minute. Parasite infection was performed by stopping the fish in the 200-liter aquarium, introducing approximately 12,000 Neo Benedenian hatchlings and exposing fish to the parasite for 1 hour. After infection, 10 fish were housed in 6 100-liter water tanks.
  • Water injection during the breeding period was 1.2 liters / minute. For 7 days after infection, they were fed commercial feed and the feeding rate was 4% of fish weight. 8 to 10 days after infection, each group was fed with the test feed. The feed rate of the test feed was 2% of the fish weight. All feeds on day 11 after infection are feeds with no added drugs. The rate was 4%. On the 12th day after infection, all fish were sampled and the number of neobenedenia parasitizing the body surface was counted.
  • Test group Administration group in which bitonol was orally administered for 3 days at 137 mg / kg fish body weight / day from the 8th day after exposure to parasite, 198 mg / kg fish body weight / day at 8 days after parasite exposure Oral administration of daily bithionol, 198 mg / kg fish body weight / day from 8 days after parasite exposure Oral administration of bithionol for 2 days from parasite exposure, 198 mg / kg fish from 8 days after parasite exposure Administration group for oral administration of bitionol for body weight / day for 3 days, from the 8th day after exposure to parasite, administration of braziquantel (commercially available anti-damaged ant for pre-types) at 162 mg / kg body weight / day for 3 days A total of 6 groups were established: a positive control group and a control group (Omg) fed with no drug-added feed. A predetermined amount of each drug was added to the moist feed to obtain a test feed.
  • bitonol In the bithonol 198 mg X 2 day treatment group, all insects that almost decreased appetite were dewormed. Based on the above results, it can be seen that bitonol has an anti-hadamushi action, and that a single dose of 137-300 mg / kg fish body weight is effective in controlling insects and does not cause a decrease in appetite. It turned out to be better than Braziquantel.
  • Test method 60 amberjacks with an average fish weight of about 43 g were raised in a 200-liter aquarium for about 7 days and acclimated to a water temperature of 25 ° C. During that time, we used commercial feed and the feeding rate was 4% of fish weight. The water injection was 2.4 liters / minute. Parasitic infection is achieved by stopping the fish in the 200-liter aquarium, introducing approximately 12,000 Neo Benedenian hatchling larvae, and exposing the parasite to fish for 1 hour. I got it. After infection, 10 fish were housed in 6 100-liter water tanks.
  • Water injection during the breeding period was 1.2 liters / minute.
  • we were fed commercial feed and the feeding rate was 4% of fish weight.
  • each group was fed with the test feed.
  • the feed rate of the test feed was 2% of the fish weight.
  • all fish were sampled and the number of neobenedenia parasitic on the body surface was counted.
  • Test group 126 mg, 147 mg, 166 mg, 185 mg, 215 mg / kg fish body weight / day on the 8th day after exposure to parasites. A total of 6 control zones (Omg) were set. A predetermined amount of each drug was added to the moist feed to make a test feed.
  • Test method 54 amberjacks with an average fish weight of about 116 g were raised in a 200-liter aquarium for about 7 days and acclimated to a water temperature of 25 ° C. During that time, we used commercial feed and the feeding rate was 3% of fish weight. The water injection was 2.4 liters / minute. Parasite infection was carried out by using a 200-liter aquarium as water, introducing about 8000 neobenedenia hatchling larvae, and exposing the parasite to fish for 1 hour.
  • each 8 fish were housed in 6 100 liter tanks. It was. In addition, the remaining 6 fish were placed in a 100 liter water tank in order to examine the length of the parasites during drug administration. Water injection during the breeding period was 1.2 liters / minute. For 7 days after infection, we were fed commercial feed and the feeding rate was 3% of fish weight. On the 8th day after the first infection, each group was fed with test feed. The feeding rate of the test feed was 2% of the fish weight.
  • Study group 81 mg, 103 mg, 125 mg, 136 mg, 157 mg / kg fish body weight / day, 8 days after exposure to parasites, fed with a single orally administered biotinol, 5 groups, fed with a drug-free feed A total of 6 control zones (Omg) were set. In addition, a section was established to determine the size of the parasites during administration of drug-added feed (6 fish contained in a 100 liter aquarium). A predetermined amount of each drug was added to the moist feed to make a test feed.
  • Judgment of effect performed by comparing appetite transition, occurrence of moribundity, and number of infestations.
  • the anthelmintic effect of bitionol on the 8 day parasite was evident from 136 mg / kg fish weight (P ⁇ 0.01), and the number of parasites in the 157 mg / kg fish weight bitionol group was zero. From the above results, it was found that the anthelmintic effect of orally administered bitionol varies depending on the size of the parasite, and that the anthelmintic effect can be obtained at a lower dose as the parasite becomes smaller. In addition, no decrease in food consumption or moribundity was observed in each test group.
  • the upper graph in Fig. 8 is a graph showing the anthelmintic effect on N.
  • girellae (body length 0.84 ⁇ 0.1 lmm) on the 4th day of parasitism
  • Test method 47 fishes with an average fish weight of about 220 g were bred in a 500 liter water tank for about 7 days and acclimated to a water temperature of 25 ° C. During that time, we used commercial feed and the feeding rate was 3% of fish weight. The water injection was 4.8 liters / minute. Parasite infection was carried out by using a 500-liter aquarium as water and introducing about 8000 neobenedenia hatchling larvae and exposing the parasite to fish for 1 hour.
  • Study group Eight days after exposure to parasites, feed biotin with no added drugs in the fifth group with orally administered bithonol at a dose of 78 mg, 93 mg, 106 mg, 123 mg, 151 mg / kg body weight / day orally. A total of 6 control zones (Omg) were set. In addition, a section was established to determine the size of the parasites during administration of drug-added feed (5 fish contained in a 200-liter aquarium). A predetermined amount of each drug was added to the moist feed to make a test feed.
  • the anthelmintic effect of bitionol on the parasite on the 8th day was clearly demonstrated by 123mg / kg fish weight strength (P 0.01), and the number of parasites in the 151mg / kg fish weight bitionol group was zero. From the above results, it was reproduced that the anthelmintic effect of orally administered bitionol differs depending on the size of the parasite, and that the anthelmintic effect can be obtained at a lower dose as the parasite becomes smaller. In each test group, food consumption and drowning were not observed.
  • the upper graph in Fig. 9 is a graph showing the anthelmintic effect on N. girellae (body length 0 ⁇ 88 ⁇ 0. 10 mm) on the 4th day of infestation.
  • Test method Ten amberjacks with an average fish weight of about 480 g were raised in a 500-liter water tank for about 7 days and acclimated to a water temperature of 25 ° C. During that time, we used commercial feed and the feeding rate was 1% of fish weight. The water injection was 4.8 liters / minute. Parasite infection was carried out with the 500-liter aquarium as still water, and about 6000 neobenedenia hatchling larvae were introduced and the parasites were exposed to fish for 1 hour. After infection, 3 fish were housed in 2 200-liter water tanks and 4 fish were housed in 1 200-liter water tank.
  • Test group 144 mg on the 8th day after exposure to parasite, 151 mg / kg body weight / day, 2 groups in the bithonol administration group, and a control group (Omg) fed with no drug-added feed. A total of 3 districts were set. The number of test animals was 4 in the 144 mg group and 3 in the 151 mg group and the control group. A predetermined amount of each drug was added to the moist feed to obtain a test feed.
  • Test method 18 fishes with an average fish weight of about 44 g were raised in a 100 liter aquarium for about 7 days and acclimated to a water temperature of 25 ° C. During that time, we used commercial feed and the feeding rate was 3% of fish weight. The water injection rate was 1.2 liters / minute. Parasitic infection was performed by putting about 3000 cubs of neo-Benedenia hatchlings in a 100-liter aquarium and exposing the parasites to fish for 1 hour. For 7 days after infection, we were fed commercial feed and the feeding rate was 3% of fish weight. On the 8th day after infection, the test diet was fed to a weight of 180 mg bitonol / kg fish.
  • the feed rate of the test feed was 2% of the fish weight.
  • the test feed was fed, and after 0, 4, 8, 12, 16, and 24 hours, 3 fish were sampled to check the number of infestations.
  • the drainage was filtered through a fine mesh, and the dropped insects were collected to examine the state of the insects.
  • the number of infestations decreased rapidly at 12 hours after administration and became almost zero at 16 hours.
  • deciduous insects in the breeding effluent should be collected 6 hours after administration.
  • the worms that had fallen were clearly cloudy throughout the worm body, and the anchors were atrophic (Fig. 12a (dropped N. girellae adults in breeding effluent 6 hours after administration)).
  • Fig. 12c adult N.
  • FIG. 12b shows a moth girellae adult (control) at 0 hours after administration.
  • Test method 10 amberjacks with an average fish weight of about 75 g were housed in three 100-liter aquariums, bred for about 7 days, and adapted to a water temperature of 25 ° C. During that time, we used commercial feed and the feed rate was 3% of fish weight. The water injection rate was 1.2 liters / minute. The test feed was fed after acclimatization. The feeding rate of the test feed was 2% of fish weight and was administered once. After that, they were fed commercial feed for 3 days, and the feeding rate was 3% of fish weight.
  • Test groups Bitonol-administered groups were administered in a single dose of 200 mg, 300 mg, 400 mg / kg body weight of fish / day and were administered orally in a single dose. A predetermined amount of each drug was added to the moist feed to make a test feed.
  • Test method 48 yellowtails with an average fish weight of about 54g were bred in a 200 liter aquarium for about 7 days and acclimated to a water temperature of 25 ° C. In the meantime, we used commercial feed and the feeding rate was 4% of fish weight. The water injection was 2.4 liters / minute. Parasite infection was achieved by using a 200-liter aquarium as water and introducing approximately 18,000 neobenedenia hatchling larvae and exposing fish to the parasite for 1 hour. After infection, 8 fish were housed in 6 100-liter water tanks. Water injection during the breeding period was 1.2 liters / minute. For 7 days after infection, they were fed commercial feed and the feeding rate was 4% of fish weight.
  • test feed was administered once on the 8th day after infection, and the feeding rate of the test feed was 2% of the fish body weight. After that, they were fed commercial feed for 3 days, and the feeding rate was 4% of the fish weight. On the 12th day after infection, all fish were sampled and the number of neobenedenia parasitizing the body surface was counted.
  • Study group 70 mg, 139 mg, 196 mg, 333 mg / kg fish body weight / day at 7 days after exposure to parasites. From 171 mg / kg fish body weight / day, a total of 6 groups, including a positive control group administered with Praziquantel (commercially available anti-stomach beetle for yellowtails) and a control group (Omg) fed with no additive feed Set. A predetermined amount of each drug was added to the moist feed to obtain a test feed.
  • Praziquantel commercially available anti-stomach beetle for yellowtails
  • 70 mg / kg B.W., 139 mg / kgB.W., 196 mg / kgB.W. (5/8 (5 out of 8) moribund) and 3 33 mg / kgB. W. (8/8 (5 out of 8) moribund) are all administered for 1 day and Praziquantel is administered for 3 days.
  • * means significant difference at p ⁇ 0.05 compared to control group (Omg)
  • * * means significant difference at p ⁇ 0.01 compared to control group (Omg).
  • Test method 20 yellowtails with an average fish weight of about 105g were bred in a 200-liter aquarium for about 7 days and acclimated to a water temperature of 25 ° C. In the meantime, we used commercial feed and the feeding rate was 3% of fish weight. The water injection was 2.4 liters / minute. Parasitic infection was achieved by using a 200-liter aquarium as water and introducing about 6000 neobenedenia hatchling larvae and exposing the parasite to fish for 1 hour. After infection, 5 fish were housed in 4 100 liter water tanks. Water injection during the breeding period was 1.2 liters / minute. For 7 days after infection, they were fed commercial feed and the feeding rate was 3% of fish weight.
  • test feed was administered every other day on the 8th and 10th days after infection, and the feeding rate of the test feed was 1%. On the 9th and 11th day after infection Feeding and the feeding rate was 3% of fish weight. On the 12th day after infection, all fish were sampled and parasitized on the body surface.
  • Test group On days 8 and 10 after exposure to parasite, 57 mg, 90 mg, 104 mg / kg fish body weight / day, ordinarily administered bithonol every other day, group 3 and fed non-drug feed A total of 4 control zones (Omg) were set. A predetermined amount of each drug was added to the moist feed to make a test feed.
  • each of the dose groups of 57 mg / kg B.W., 90 mg / kgB.W., And 104 mg / kgB.W. Is administered every other day for 2 days.
  • Test method Three yellowtails each having an average fish weight of about 650 g were housed in three 500-liter aquariums, bred for about 7 days, and adapted to a water temperature of 25 ° C. During that time, we used commercial feed and the feed rate was 1% of the fish weight. The water injection was 4.8 liters / minute. The test feed was fed after acclimatization. The feeding rate of the test feed was a single dose at 0.5% fish weight. After that, they were given a commercial feed for 8 days, and the feeding rate was 1% of fish weight.
  • Test groups 200 mg, 300 mg / kg fish body weight / day, 2 groups for the administration of vitamin with a single oral administration of bithonol, and 3 groups for the control group (Omg) with no drug-added feed were set. . A predetermined amount of each drug was added to the moist feed to obtain a test feed.
  • Judgment of effect It was performed by comparing the occurrence of drowning and appetite transition.
  • an antiparasitic agent capable of controlling body surface parasites such as cultured fish by oral administration. By adjusting the dose and the administration period, the effect can be exerted without causing side effects of bitionol.

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Abstract

Le procédé décrit permet de supprimer les parasites se trouvant sur la surface corporelle des poissons sans engendrer d'effets secondaires, en administrant aux poissons par voie orale du bithionol dans une quantité de 50 à 250 mg/kg de poids corporel par jour. Le bithionol est administré par voie orale pendant 1 à 3 jours, tous les jours ou tous les deux jours. Ce procédé convient aux poissons de l'ordre des Perciformes, des Pleuronectiformes et des Tetraodontiformes, tels que les sériole, limande à queue jaune, sériole lalandi, sériole limon, cobia, chinchard commun, chinchard du Japon, perche japonaise, Pagrus major, Zebrasoma flavescens, Epinephelus akaara, Epinephelus bruneus, cardeau hirame, Verasper moseri, Takifugu rubripes, Oplegnathus fasciatus, Oplegnathus punctatus, Scomber japonicus, Stephanolepis cirrhifer, Scophthalmus aquosus et tilapia, qui sont parasités par Neobenedenia girellae. Lors de la suppression des parasites sur la surface corporelle des poissons, il est possible d'utiliser des aliments pour poissons auxquels est ajoutée la quantité nécessaire de bithionol.
PCT/JP2007/064699 2006-07-26 2007-07-26 Procédé de suppression de parasites sur la surface corporelle de poissons WO2008013235A1 (fr)

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KR100989384B1 (ko) * 2009-08-14 2010-10-25 주식회사 씨에버 생육가능한 4배체 굴을 생산하는 방법

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CN112471041B (zh) * 2020-11-02 2023-02-24 海南省海洋与渔业科学院 一种鞍带石斑鱼养殖水环境盐度调节装置及低盐养殖方法

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HATAI K. ET AL.: "Yoshoku Madai ni Kisei suru Kubinaga Kotochu no Kujo Shiken", BULLETIN OF NAGASAKI PREFECTURAL INSTITUTE OF FISHERIES, no. 9, 1983, pages 31 - 35, XP003020844 *
KIM K.-H. ET AL.: "Treatment of Microcotyle sebastis (Monogenea) on the gills of cultured rockfish (Sebastes schelegeli) with oral administration of mebendazole and bithionol", AQUACULTURE, vol. 167, no. 1,2, 1998, pages 115 - 121, XP003020843 *
TAKADA T. ET AL.: "Hamachi no Gaibu Kisei Kyuchu no Kujo ni Kansuru Kenkyu", AQUACULTUER SCIENCE, vol. 13, no. 1, 1965, pages 23 - 27, XP003020845 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100989384B1 (ko) * 2009-08-14 2010-10-25 주식회사 씨에버 생육가능한 4배체 굴을 생산하는 방법

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