WO2007141888A1 - Appareil de fixation d'un tissu biologique - Google Patents

Appareil de fixation d'un tissu biologique Download PDF

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Publication number
WO2007141888A1
WO2007141888A1 PCT/JP2006/317787 JP2006317787W WO2007141888A1 WO 2007141888 A1 WO2007141888 A1 WO 2007141888A1 JP 2006317787 W JP2006317787 W JP 2006317787W WO 2007141888 A1 WO2007141888 A1 WO 2007141888A1
Authority
WO
WIPO (PCT)
Prior art keywords
biological tissue
formalin
fixing
tank
cleaning
Prior art date
Application number
PCT/JP2006/317787
Other languages
English (en)
Japanese (ja)
Inventor
Yasuhiko Kitayama
Kimihiko Yamada
Hiroshi Moro
Tsuyoshi Takatera
Original Assignee
Jokoh Co., Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jokoh Co., Ltd filed Critical Jokoh Co., Ltd
Publication of WO2007141888A1 publication Critical patent/WO2007141888A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor

Definitions

  • the present invention relates to a biological tissue fixing device used in a biological tissue specimen preparation process for pathological examination, particularly when biological tissue is fixed and washed.
  • Non-Patent Document 1 Kiminobu Kanno, Takuya Moriya, Toshiaki Manabe, “Guide to Pathology Laboratory for Clinicians and Early Residents”, Bunkodo, May 26, 2004, pl6—19
  • the present invention has been made in view of the above-described problems, and fixing force of a biological tissue to be removed at the time of operation does not require manual operation in the process until washing, and prevents fixation of formalin on an operator. It is an object to provide a device.
  • a biological immobilization apparatus comprising a function of immobilizing a biological tissue and a function of washing the biological tissue and performing the fixing force washing continuously is configured. It is. That is, it can be carried out continuously from a fixing step in which biological tissue extracted at the time of surgery is immersed in formalin to a washing step in which formalin that has permeated biological tissue is washed away.
  • the present invention provides a biological tissue fixing device characterized in that the fixing force is performed in the same processing tank until washing.
  • an apparatus is provided that can continuously carry out the steps from the fixed cap to the cleaning in one processing tank.
  • a biological tissue fixing device wherein a lid is provided on the treatment tank. That is, an apparatus is provided in which a lid is attached to the same treatment tank when the fixing force and the process up to washing are continuously performed.
  • this apparatus continuously performs a washing process in which formalin infiltrated into a biological tissue is washed and flowed from a fixing process in which the formalin fixing solution is permeated into the biological tissue, this results in the need for manual operation. It does not adhere to workers. If scouring, fixing, and cleaning are performed in the same processing tank, both processes are performed effectively. In addition, if a lid is provided in the treatment tank, the confidentiality of the tank is increased, and the risk of formaldehyde leaking due to vaporization of formalin and formalin is minimized.
  • FIG. 1 is a perspective view of a biological tissue fixing device according to the present invention. It consists of treatment tank 1 and tank part 2. [0012] A lid 3 is provided on the upper part of the treatment tank 1, so that formaldehyde generated in the treatment tank is not leaked.
  • An operation panel 4 is installed in the tank unit 2, and operations related to processing can be performed.
  • a BOX-shaped space is provided at the bottom of the operation panel 4, and a tank 5 for storing formalin can be stored.
  • a caster 6 is attached to the bottom surface of the tank part 2 and can be easily moved.
  • FIG. 2 is a cross-sectional view of the biological yarn and weave fixing device as seen from the right side.
  • a biological tissue fixing plate 7 to which a biological tissue is attached can be installed in the treatment tank 1.
  • an upper sensor 8 for detecting the liquid level, a lower sensor 9 for confirming whether the solution has entered the processing tank 1, and a formalin inlet 10 for injecting formalin are installed on the side surface of the processing tank 1. ing.
  • a filter 11 that can filter formalin containing dust residue that generates biological tissue force at the time of fixation, drains formalin after the fixation step, and injects a cleaning solution.
  • a liquid injection outlet 12 that can be used, and a cleaning liquid outlet 13 that discharges the cleaning liquid after cleaning are provided.
  • This cleaning liquid discharge port 13 uses the principle of siphon.
  • the tank unit 2 includes an upper liquid injection / discharge unit 14 and a lower tank installation base 15.
  • the liquid injection / discharge unit 14 is provided with a pump 16 for injecting formalin in the tank 5 into the treatment tank 1, a cleaning liquid inlet 17 for drawing the cleaning liquid into the apparatus, and a pressure reducing valve 18 for controlling the water flow of the cleaning liquid. ing.
  • a pump 16 for injecting formalin in the tank 5 into the treatment tank 1
  • a cleaning liquid inlet 17 for drawing the cleaning liquid into the apparatus
  • a pressure reducing valve 18 for controlling the water flow of the cleaning liquid.
  • tap water is used as a cleaning solution for biological tissues.
  • the cleaning liquid inlet 17 is constructed by connecting a tap and a hose.
  • the tank installation base 15 can be pulled out in the left direction of FIG. 2 to facilitate the installation of the tank 5.
  • FIG. 3 is a cross-sectional view of the biological yarn and weave fixing device as seen from the left side.
  • a formalin discharge solenoid valve 19 for returning formalin in the treatment tank 1 to the tank 5 and a washing liquid injection solenoid valve 20 for controlling the washing liquid injection into the treatment tank 1 during cleaning are installed.
  • FIG. 4 is an enlarged view of the operation panel 4.
  • the main power source 21 of the biological tissue fixing device On the surface of the operation panel 4, the main power source 21 of the biological tissue fixing device, the power LED 22 that lights up when the main power source 21 is ON, the stop switch 23 that can interrupt the process, the start switch 24 that starts the process, Cleaning timer 25 that can set the cleaning time arbitrarily, Alarm LED 26 that lights up and reports when a failure such as clogging of filter 11 occurs, Fixed timer 27 that can set the fixed time arbitrarily, Processing tank If formalin remains in 1 and the process stops, the formalin in the treatment tank 1 can be returned to the formalin tank 8 by using the formalin discharge switch 28, which contains formalin in the treatment tank 1.
  • Formalin LED29 which is lit while the cleaning tank is in the process tank, or when the process is interrupted, or when the process is completed, the cleaning liquid in the process tank can be discharged.
  • the liquid discharge switch 30 and the end LED 31 indicating that the process has been completed are installed!
  • the cleaning liquid used in the description examples may be a solution made for cleaning biological tissues, such as pure water or physiological saline, which is not limited to tap water.
  • the cleaning method is not limited to the mechanism using the principle of siphon.
  • the pump is driven and the cleaning liquid is discharged. Similar to the mechanism using the principle of the siphon in the example described, even if the overflow is provided in tank 1 and the cleaning liquid accumulates in the processing tank 1 above a certain level, the cleaning liquid above a certain level is discharged from the overflow to the drain. Needless to say, the cleaning effect can be obtained.
  • the biological tissue removed by the operation is split as it is, with a force of about 1 to 2 cm.
  • luminal organs such as the stomach and large intestine are incised and pinned to a plate and attached to the biological tissue fixing plate 7 shown in FIG.
  • the biological tissue is set in treatment tank 1 of Fig. 1 and described in Fig. 4.
  • the power supply LED 22 in FIG. 4 lights up.
  • the fixed timer 27 sets the fixed process time, and the cleaning timer 25 sets the cleaning time.
  • the action of the fixed timer 27 prevents the biological tissue from being over-fixed (if the state is too fixed, the state of the biological tissue section to be produced becomes worse), and the cleaning timer 25 saves extra water bills. Is possible.
  • the cleaning timer 25 of FIG. 4 is driven to count the set time, and the cleaning liquid electromagnetic valve 20 (see FIG. 3) is opened, and the tap water is treated as shown in FIG. It is poured into tank 1.
  • the tap water reaches a certain level in the treatment tank, all the liquid in the treatment tank 1 is discharged from the cleaning liquid discharge port 13 in FIG.
  • the biological tissue is washed repeatedly by storing and discharging tap water until the set time has elapsed.
  • the biological yarn and weaving can be prevented from drying, and removed from the treatment tank 1 at a time convenient for the operator. Can be processed.
  • the cleaning liquid discharge switch 30 shown in Fig. 4 is pressed to discharge all the tap water stored in the treatment tank, and then the biological tissue is taken out.
  • a water leak sensor is installed. When a liquid leak occurs during processing, it is detected, and when the processing is interrupted, the alarm LED 26 shown in Fig. 4 is turned on and a buzzer sounds.
  • a time-out was provided for the time for injecting and discharging the solution to the treatment tank 1 shown in FIG. If the water level does not reach a certain level even after a certain period of time when the solution is injected into the processing tank 1, the upper sensor 8 shown in Fig. 2 detects it and immediately stops processing, and the alarm shown in Fig. 4 is displayed. A buzzer sounds when LED26 lights up.
  • the biological tissue extracted by surgery or the like has blood, unnecessary tissue pieces, etc. attached thereto, so that formalin used at the time of fixation becomes soiled and formalized. Shorten marine life. Therefore, as a pretreatment step for fixation, a step of washing a living tissue with a washing liquid may be included separately from the present invention. By performing this pretreatment step, the life of formalin can be extended.
  • the present invention is not limited to the above embodiment, and the design can be changed as appropriate, such as the height of the apparatus and the size of the treatment tank.
  • FIG. 1 is a perspective view of an apparatus of the present invention.
  • FIG. 2 is a cross-sectional view of the device of the present invention viewed from the right side.
  • FIG. 3 is a cross-sectional view of the device of the present invention as viewed from the left side.
  • FIG. 4 is an operation panel diagram of the device of the present invention. Explanation of symbols
  • Formalin discharge switch Formalin LED Cleaning liquid discharge switch End LED

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Cleaning By Liquid Or Steam (AREA)

Abstract

La présente invention concerne l'élaboration d'un appareil hermétique de fixation de tissu biologique qui n'exige pas de manipulation au cours des opérations allant de la fixation de tissu biologique, prélevé lors d'une intervention chirurgicale, au lavage du tissu. Le dépôt de formaline sur le manipulateur est de ce fait évité. L'invention concerne donc un appareil de fixation de tissu biologique caractérisé en ce que l'appareil ne sert pas seulement à fixer un tissu biologique mais également à laver ledit tissu biologique, effectuant ainsi en continu les opérations qui vont de la fixation au lavage. Autrement dit, l'opération de fixation, impliquant l'immersion dans la formaline, jusqu'à l'opération de lavage, destinée à éliminer la formaline qui a imprégné le tissu biologique, peuvent être mises en œuvre de manière continue en plaçant un tissu biologique, prélevé au cours d'une intervention chirurgicale, dans le récipient de traitement (1) et en pressant le commutateur de mise en service (24).
PCT/JP2006/317787 2006-06-05 2006-09-07 Appareil de fixation d'un tissu biologique WO2007141888A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2006155849 2006-06-05
JP2006-155849 2006-06-05

Publications (1)

Publication Number Publication Date
WO2007141888A1 true WO2007141888A1 (fr) 2007-12-13

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2006/317787 WO2007141888A1 (fr) 2006-06-05 2006-09-07 Appareil de fixation d'un tissu biologique

Country Status (1)

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WO (1) WO2007141888A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001516869A (ja) * 1997-08-20 2001-10-02 ザユニバーシティー オブ マイアミ 高品質、連続処理、組織固定−脱水−脱脂−含浸方法
JP2003517601A (ja) * 1999-12-14 2003-05-27 ユニバーシティー・オブ・マイアミ 迅速な組織処理器
JP2005345197A (ja) * 2004-06-01 2005-12-15 Yoshihiko Yamada 超音波を使った生体組織の迅速処理法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001516869A (ja) * 1997-08-20 2001-10-02 ザユニバーシティー オブ マイアミ 高品質、連続処理、組織固定−脱水−脱脂−含浸方法
JP2003517601A (ja) * 1999-12-14 2003-05-27 ユニバーシティー・オブ・マイアミ 迅速な組織処理器
JP2005345197A (ja) * 2004-06-01 2005-12-15 Yoshihiko Yamada 超音波を使った生体組織の迅速処理法

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