WO2007141888A1

WO2007141888A1 - Biological tissue fixing apparatus

Info

Publication number: WO2007141888A1
Application number: PCT/JP2006/317787
Authority: WO
Inventors: Yasuhiko Kitayama; Kimihiko Yamada; Hiroshi Moro; Tsuyoshi Takatera
Original assignee: Jokoh Co., Ltd
Priority date: 2006-06-05
Filing date: 2006-09-07
Publication date: 2007-12-13

Abstract

[PROBLEMS] To provide a hermetically sealed biological tissue fixing apparatus that does not need labor during the operations from fixing of biological tissue removed in surgery to washing thereof, thereby avoiding any formalin attachment to the worker. [MEANS FOR SOLVING PROBLEMS] There is provided a biological tissue fixing apparatus characterized in that the apparatus has not only the function of fixing a biological tissue but also the function of washing the biological tissue, thereby continuously carrying out fixing to washing operations. That is, the fixing operation for formalin immersion to washing operation for washing away of formalin having infiltrated the biological tissue can be continuously carried out by placing a biological tissue removed in surgery in treatment vessel (1) and pressing start switch (24).

Description

Specification

Biological tissue fixation device

Technical field

TECHNICAL FIELD [0001] The present invention relates to a biological tissue fixing device used in a biological tissue specimen preparation process for pathological examination, particularly when biological tissue is fixed and washed.

Background art

[0002] Conventionally, for preparation of a biological tissue section for pathological diagnosis, formalin is used as a fixing solution, alcohol is used as a dehydrating agent, and xylene is used as an intermediate treatment agent. After passing through steps such as an intermediate treatment step with xylene and a paraffin permeation step with paraffin, the filling of the raffin is performed to produce the noraffin block. Then, the paraffin block is sliced, attached to a slide glass, deparaffinized with xylene, dexylene, dealcoholized, etc., stained with various dyeing methods, and covered with a cover glass.

[0003] When a biological tissue section is prepared from a biological tissue (stomach, liver, spleen, lung, etc.) removed at the time of surgery, the fixing process described in the previous section changes. Leave the living organs such as the liver and lungs as they are, or split (make a cut) the biological tissue to a thickness of about 12 cm before fixation. In addition, a hollow organ such as the stomach or large intestine is incised and pinned to a plate made of silicone rubber or the like (for example, see Patent Document 1). Then, immerse and fix in formalin for several days. Since the biological tissue after fixation generates a very large amount of formaldehyde, it is washed with water for 2-3 hours after removing the formalin power. Thereafter, the biological tissue is split so as to have a size suitable for the production of paraffin blocks, and a normal alcohol dehydration step is performed (for example, see Non-Patent Document 1). Patent Document 1: Registered Utility Model No. 3012491

Non-Patent Document 1: Kiminobu Kanno, Takuya Moriya, Toshiaki Manabe, “Guide to Pathology Laboratory for Clinicians and Early Residents”, Bunkodo, May 26, 2004, pl6—19

[0004] However, with this method, manual operation is required to remove the fixed biological tissue from the formalin solution and wash it with water, and there is a high possibility that harmful formalin will adhere to the human body. . Disclosure of the invention

Problems to be solved by the invention

[0005] The present invention has been made in view of the above-described problems, and fixing force of a biological tissue to be removed at the time of operation does not require manual operation in the process until washing, and prevents fixation of formalin on an operator. It is an object to provide a device.

Means for solving the problem

[0006] In order to solve the above-mentioned problems, a biological immobilization apparatus comprising a function of immobilizing a biological tissue and a function of washing the biological tissue and performing the fixing force washing continuously is configured. It is. That is, it can be carried out continuously from a fixing step in which biological tissue extracted at the time of surgery is immersed in formalin to a washing step in which formalin that has permeated biological tissue is washed away.

[0007] Further, the present invention provides a biological tissue fixing device characterized in that the fixing force is performed in the same processing tank until washing. In other words, an apparatus is provided that can continuously carry out the steps from the fixed cap to the cleaning in one processing tank.

[0008] Furthermore, a biological tissue fixing device is provided, wherein a lid is provided on the treatment tank. That is, an apparatus is provided in which a lid is attached to the same treatment tank when the fixing force and the process up to washing are continuously performed.

The invention's effect

[0009] Since this apparatus continuously performs a washing process in which formalin infiltrated into a biological tissue is washed and flowed from a fixing process in which the formalin fixing solution is permeated into the biological tissue, this results in the need for manual operation. It does not adhere to workers. If scouring, fixing, and cleaning are performed in the same processing tank, both processes are performed effectively. In addition, if a lid is provided in the treatment tank, the confidentiality of the tank is increased, and the risk of formaldehyde leaking due to vaporization of formalin and formalin is minimized.

BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, the best embodiment of the present invention will be described with reference to the drawings.

FIG. 1 is a perspective view of a biological tissue fixing device according to the present invention. It consists of treatment tank 1 and tank part 2. [0012] A lid 3 is provided on the upper part of the treatment tank 1, so that formaldehyde generated in the treatment tank is not leaked.

[0013] An operation panel 4 is installed in the tank unit 2, and operations related to processing can be performed. In addition, a BOX-shaped space is provided at the bottom of the operation panel 4, and a tank 5 for storing formalin can be stored. Furthermore, a caster 6 is attached to the bottom surface of the tank part 2 and can be easily moved.

FIG. 2 is a cross-sectional view of the biological yarn and weave fixing device as seen from the right side. In the treatment tank 1, a biological tissue fixing plate 7 to which a biological tissue is attached can be installed. In addition, an upper sensor 8 for detecting the liquid level, a lower sensor 9 for confirming whether the solution has entered the processing tank 1, and a formalin inlet 10 for injecting formalin are installed on the side surface of the processing tank 1. ing.

[0015] Furthermore, on the bottom surface of the treatment tank 1, a filter 11 that can filter formalin containing dust residue that generates biological tissue force at the time of fixation, drains formalin after the fixation step, and injects a cleaning solution. A liquid injection outlet 12 that can be used, and a cleaning liquid outlet 13 that discharges the cleaning liquid after cleaning are provided. This cleaning liquid discharge port 13 uses the principle of siphon. When the cleaning liquid injected from the liquid injection / discharge port 12 accumulates above the cleaning liquid discharge port 13 and above the loop, all the cleaning liquid in the treatment tank 1 is It is structured to be discharged to a drainage facility such as an external sink. For this reason, the bottom surface of the treatment tank 1 needs to be higher from the floor than the bottom surface of the drain.

The tank unit 2 includes an upper liquid injection / discharge unit 14 and a lower tank installation base 15.

[0017] The liquid injection / discharge unit 14 is provided with a pump 16 for injecting formalin in the tank 5 into the treatment tank 1, a cleaning liquid inlet 17 for drawing the cleaning liquid into the apparatus, and a pressure reducing valve 18 for controlling the water flow of the cleaning liquid. ing. Generally, tap water is used as a cleaning solution for biological tissues. For this reason, the cleaning liquid inlet 17 is constructed by connecting a tap and a hose.

[0018] The tank installation base 15 can be pulled out in the left direction of FIG. 2 to facilitate the installation of the tank 5.

FIG. 3 is a cross-sectional view of the biological yarn and weave fixing device as seen from the left side. In the center of the figure, after the fixing process, a formalin discharge solenoid valve 19 for returning formalin in the treatment tank 1 to the tank 5 and a washing liquid injection solenoid valve 20 for controlling the washing liquid injection into the treatment tank 1 during cleaning are installed. Yes. FIG. 4 is an enlarged view of the operation panel 4. On the surface of the operation panel 4, the main power source 21 of the biological tissue fixing device, the power LED 22 that lights up when the main power source 21 is ON, the stop switch 23 that can interrupt the process, the start switch 24 that starts the process, Cleaning timer 25 that can set the cleaning time arbitrarily, Alarm LED 26 that lights up and reports when a failure such as clogging of filter 11 occurs, Fixed timer 27 that can set the fixed time arbitrarily, Processing tank If formalin remains in 1 and the process stops, the formalin in the treatment tank 1 can be returned to the formalin tank 8 by using the formalin discharge switch 28, which contains formalin in the treatment tank 1. Formalin LED29, which is lit while the cleaning tank is in the process tank, or when the process is interrupted, or when the process is completed, the cleaning liquid in the process tank can be discharged. The liquid discharge switch 30 and the end LED 31 indicating that the process has been completed are installed!

[0021] With these functions, it is possible to continuously perform the fixing step and the cleaning step in the same processing tank.

[0022] Although the fixing process and the cleaning process are performed in the same processing tank in the description example, the fixing process and the cleaning process are continuously performed even if the respective processes are performed with separate apparatuses. It goes without saying that doing this will result in similar results.

[0023] In addition, the cleaning liquid used in the description examples may be a solution made for cleaning biological tissues, such as pure water or physiological saline, which is not limited to tap water.

[0024] Further, the cleaning method is not limited to the mechanism using the principle of siphon. For example, when the cleaning liquid is accumulated in the processing tank 1, the pump is driven and the cleaning liquid is discharged. Similar to the mechanism using the principle of the siphon in the example described, even if the overflow is provided in tank 1 and the cleaning liquid accumulates in the processing tank 1 above a certain level, the cleaning liquid above a certain level is discharged from the overflow to the drain. Needless to say, the cleaning effect can be obtained.

Next, actual processing steps will be described.

[0026] The biological tissue removed by the operation is split as it is, with a force of about 1 to 2 cm. In addition, luminal organs such as the stomach and large intestine are incised and pinned to a plate and attached to the biological tissue fixing plate 7 shown in FIG. Next, the biological tissue is set in treatment tank 1 of Fig. 1 and described in Fig. 4. When the main power supply 21 of the biological tissue fixing device is turned ON, the power supply LED 22 in FIG. 4 lights up.

[0027] Using the operation panel in FIG. 4, the fixed timer 27 sets the fixed process time, and the cleaning timer 25 sets the cleaning time. The action of the fixed timer 27 prevents the biological tissue from being over-fixed (if the state is too fixed, the state of the biological tissue section to be produced becomes worse), and the cleaning timer 25 saves extra water bills. Is possible.

When the start switch 24 in FIG. 4 is pressed, the pump 16 shown in FIG. 2 is driven, and 10% to 20% buffered formalin is injected into the treatment tank 1 shown in FIG. When formalin reaches a certain water level in the treatment tank, the top sensor 8 (see Fig. 2) stops formalin injection.

[0029] When the start switch 24 shown in FIG. 4 is pushed with the liquid in the processing tank 1 shown in FIG. 1, the upper sensor 8 and the lower sensor 9 shown in FIG. Even if the start switch 24 is pressed, the start signal becomes invalid and the process is not started.

[0030] When formalin injection into the treatment tank 1 described in FIG. 1 stops, the fixed timer 27 (see FIG. 4) is activated, and the biological tissue in the treatment tank 1 is fixed by waiting for the set time. .

[0031] When the set time of the fixed timer 27 (see Fig. 4) elapses, the formalin discharge solenoid valve 19 shown in Fig. 3 opens, and the formalin in the treatment tank 1 shown in Fig. 1 is filtered by the filter shown in Fig. 2. Pass 11 and return to tank 5 (see Fig. 1). After fixation, formalin passes through the filter 11 to filter dirt (tissue fragments, etc.), extending the life of formalin.

[0032] When all of the formalin returns to the tank 5, the lower sensor 9 shown in Fig. 2 detects it, and the formalin discharge solenoid valve 19 shown in Fig. 3 closes.

[0033] Next, the cleaning timer 25 of FIG. 4 is driven to count the set time, and the cleaning liquid electromagnetic valve 20 (see FIG. 3) is opened, and the tap water is treated as shown in FIG. It is poured into tank 1. When the tap water reaches a certain level in the treatment tank, all the liquid in the treatment tank 1 is discharged from the cleaning liquid discharge port 13 in FIG.

[0034] By the above method, the biological tissue is washed repeatedly by storing and discharging tap water until the set time has elapsed.

[0035] When the time set by the cleaning timer 25 shown in Fig. 4 has elapsed, tap water is injected until the upper sensor 8 shown in Fig. 2 detects it, and the tap water has accumulated in the treatment tank 1 of Fig. 1. End processing with End, as shown in Fig. 4, turns on the end LED31 and the buzzer sounds for a certain period of time

[0036] By ending the treatment with the treatment tank 1 in FIG. 1 filled with tap water, the biological yarn and weaving can be prevented from drying, and removed from the treatment tank 1 at a time convenient for the operator. Can be processed.

[0037] After the treatment is completed, the cleaning liquid discharge switch 30 shown in Fig. 4 is pressed to discharge all the tap water stored in the treatment tank, and then the biological tissue is taken out.

[0038] As a safety function, a water leak sensor is installed. When a liquid leak occurs during processing, it is detected, and when the processing is interrupted, the alarm LED 26 shown in Fig. 4 is turned on and a buzzer sounds.

[0039] As another safety function, a time-out was provided for the time for injecting and discharging the solution to the treatment tank 1 shown in FIG. If the water level does not reach a certain level even after a certain period of time when the solution is injected into the processing tank 1, the upper sensor 8 shown in Fig. 2 detects it and immediately stops processing, and the alarm shown in Fig. 4 is displayed. A buzzer sounds when LED26 lights up.

[0040] Similarly, when discharging, if the solution force does not completely escape from the treatment tank 1 shown in Fig. 1 and the lower sensor 9 (see Fig. 2) cannot be detected even after a certain period of time, the process is immediately interrupted. The alarm LED26 shown in Fig. 4 is turned on and the buzzer sounds.

[0041] Although not included in the above-described processing steps, the biological tissue extracted by surgery or the like has blood, unnecessary tissue pieces, etc. attached thereto, so that formalin used at the time of fixation becomes soiled and formalized. Shorten marine life. Therefore, as a pretreatment step for fixation, a step of washing a living tissue with a washing liquid may be included separately from the present invention. By performing this pretreatment step, the life of formalin can be extended.

[0042] Furthermore, the present invention is not limited to the above embodiment, and the design can be changed as appropriate, such as the height of the apparatus and the size of the treatment tank.

Brief Description of Drawings

[0043] FIG. 1 is a perspective view of an apparatus of the present invention.

FIG. 2 is a cross-sectional view of the device of the present invention viewed from the right side.

FIG. 3 is a cross-sectional view of the device of the present invention as viewed from the left side. FIG. 4 is an operation panel diagram of the device of the present invention. Explanation of symbols

1 Treatment tank

2 Tank tank

3 lid

4 Control panel

5 tanks

6 casters

7 Biological tissue fixation plate

8 Upper sensor

9 Bottom sensor

10 Formalin inlet

11 Huinoleta

12 Liquid injection outlet

13 Cleaning fluid outlet

14 Liquid injection / discharge unit

15 Tank installation stand

16 pump

17 Cleaning fluid inlet

18 Pressure reducing valve

19 Formalin discharge solenoid valve

20 Cleaning fluid injection solenoid valve

21 Main power supply

22 Power LED

23 Stop switch

24 Start switch

25 Cleaning timer

26 Alarm LED Fixed timer

Formalin discharge switch Formalin LED Cleaning liquid discharge switch End LED

Claims

The scope of the claims

[1] A biological tissue fixing device having a function of fixing biological tissue and a function of cleaning biological tissue, and performing from fixing to cleaning continuously.

2. The biological yarn and weaving fixing device according to claim 1, wherein the steps up to the fixing force washing are performed in the same processing tank.

3. The biological tissue fixing device according to claim 2, wherein the treatment tank is provided with a lid.