WO2007086990A2 - Modulation of gene expression by oligomers targeted to chromosomal dna - Google Patents

Modulation of gene expression by oligomers targeted to chromosomal dna Download PDF

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WO2007086990A2
WO2007086990A2 PCT/US2006/044042 US2006044042W WO2007086990A2 WO 2007086990 A2 WO2007086990 A2 WO 2007086990A2 US 2006044042 W US2006044042 W US 2006044042W WO 2007086990 A2 WO2007086990 A2 WO 2007086990A2
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oligomer
target
gene
promoter
cell
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WO2007086990A3 (en
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David Reid Corey
David S. Shames
Bethany A. Janowski
John D. Minna
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University of Texas System
University of Texas at Austin
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University of Texas System
University of Texas at Austin
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Priority to JP2008541255A priority patent/JP5066095B2/ja
Priority to CA002629664A priority patent/CA2629664A1/en
Publication of WO2007086990A2 publication Critical patent/WO2007086990A2/en
Publication of WO2007086990A3 publication Critical patent/WO2007086990A3/en
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Definitions

  • the field of the invention is modulating gene transcript synthesis using polynucleotide oligomers targeting a promoter region of the gene.
  • RNAi post-transcriptional RNA interference
  • RNA-directed DNA methylation was described originally in plants (Matzke et al 2004), where it was found that RNA viruses and viroids could induce methylation in genomic DNA sequences (Massenegger et al 1994). Methylated bases were concentrated within sequences of DNA that were complementary to RNA, suggesting a sequence-specific mechanism for recognition (Pelissier and Wassenegger 2000).
  • RNAs that target centromere repeat sequences and mating type loci can silence gene expression by promoting modification of heterochromatin (Grewal and Moazed 2003; Bernstein and Allis 2005). Chromatin modifications involve methylation of histone H3 at Lysine 9 (Volpe et al 2002) and require RNA-dependent RNA polymerase (Sugiyama et al 2005) and DNA polymerase II (Schramke et al 2005). Modification involves proteins of the RNA-induced transcriptional silencing (RITS) pathway (Verdel et al 2003) including argonaute 1 (Sigova et al 2004), a member of a protein family that is also involved in post-transcriptional silencing.
  • RVS RNA-induced transcriptional silencing
  • RNAs antigene RNAs
  • Kawasaki and Taira targeted ten duplex RNAs to sequences within the E-cadherin promoter that contained CpG dinucleotides (Kawasaki and Taira, 2004). DNA methylation was observed at all of these sites. Individual RNAs yielded only marginal reductions in E- cadherin expression but more complete silencing could be achieved if all ten RNAs were combined. A link between methylation and silencing was supported by the observation that duplex RNAs were not able to inhibit expression of E-cadherin when methyl-transferase genes DMNTl and DMNT3B were silenced.
  • RNA-directed methylation in mammalian cells have been less successful. Steer and coworkers tested RNAs that targeted the gene encoding Huntingtin and did not detect any methylation (Park et al 2004). No RNA-directed methylation was observed upon stable expression of double-stranded RNA in mouse oocytes (Svoboda, P. et al 2004). Rossi and colleagues used expressed short hairpin RNAs (shRNAs) to target a well- characterized CpG island within the promoter for the tumor suppressor RASSFlA (Castanotto et al 2005). They reported modest inhibition of gene expression. The methylation-specific PCR assay showed methylation, but the more complete bisulphite sequencing assay did not.
  • RNA-mediated silencing of chromosomal DNA can be achieved independent of DNA methylation (Janowski et al 2005; US Pat appl no. 60/661,769).
  • a practical advantage of targeting transcription start sites is that they occur in all genes and provide a general and predictable class of target sequences; targeting transcription start sites would also be expected to block gene expression regardless of whether methylation occurs.
  • siRNAs targeting the E-cadherin gene promoter can activate transcription (Li et al, 2005) in cultured breast cancer cells.
  • data has been presented indicating increased EFlA mRNA expression by promoter-targeted siRNA (Morris et al 2004; see Fig. 3A, first two bars).
  • ds nuclear localized small modulatory double-stranded
  • smRNA nuclear localized small modulatory double-stranded RNA
  • One aspect of the invention is a method of selectively increasing synthesis of a target transcript of a gene in a mammalian cell, wherein the target transcript is predetermined to be in need of increased synthesis, the method comprising the steps of: contacting the cell with a polynucleotide oligomer of 12-28 bases complementary to a region within a target promoter of the gene under conditions whereby the oligomer selectively increases synthesis of the target transcript; and detecting resultant selective increased synthesis of the target gene.
  • the region is located between nucleotides -100 to +25 relative to a transcription start site of the gene.
  • the region is located between nucleotides -50 to +25, -30 to +17, and -15 to +10, relative to a transcription start site of the gene. In a particular embodiment, the region includes nucleotides -9 to +2 relative to a transcription start site of the gene. In a particular embodiment, the region includes a transcription start site of the gene. [020] In one embodiment, the target promoter is the promoter of the target transcript. In another embodiment, the target promoter is the promoter of an isoform of the target transcript. Tn further embodiments, the target promoter is both the promoter of the target transcript and the promoter of an isoform of the target transcript.
  • the oligomer is selected from the group consisting of a double- stranded RNA, a DNA, a peptide nucleic acid, and a morpholino.
  • the oligomer is a double-stranded RNA of 18-25 bases.
  • the oligomer comprises a nucleotide having a 2' chemical modification.
  • the oligomer comprises a serum stability-enhancing chemical modification selected from the group consisting of a phosphorothioate internucleotide linkage, a 2'-O-methyl ribonucleotide, a 2'-deoxy-2'-fluoro ribonucleotide, a 2'-deoxy ribonucleotide, a universal base nucleotide, a 5-C-methyl nucleotide, an inverted deoxyabasic residue incorporation, and a locked nucleic acid.
  • the cell is a cultured cell in vitro. In other embodiments, the cell is in situ in a host.
  • the contacting step is free of viral transduction. In further embodiments, the contacting step is free of viral transduction, and the cell is contacted with a composition consisting essentially of the oligomer. In a further embodiment, the contacting step is free of viral transduction, and there is at least a 2-fold resultant increased synthesis of the target transcript. In another embodiment, the oligomer is a double-stranded RNA of 18- 25 bases, a single region of the target promoter is targeted, and there is at least a 2-fold resultant increased synthesis of the target transcript. In another embodiment the contacting step is free of viral transduction, and the oligomer is not attached to a nuclear localization peptide.
  • the cell is contacted with a 1-100 nanomolar concentration of the oligomer.
  • the cell is a cancer cell and the gene encodes a protein selected from the group consisting of E-cadhcrin, human progesterone receptor (hPR), p53, and PTEN.
  • hPR human progesterone receptor
  • Another aspect of the invention is an isolated or synthetic polynucleotide oligomer for selectively increasing synthesis of a target transcript of a gene, the oligomer comprising a nucleotide sequence of 12-28 bases complementary to a region within a target promoter of the gene, wherein introduced into a cell comprising the gene the oligomer selectively increases transcription of the target transcript.
  • the region is located between nucleotides -100 to +25 relative to a transcription start site of the gene.
  • the region is located between nucleotides -50 to +25, -30 to +17, and -15 to +10, relative to a transcription start site of the gene.
  • the region includes nucleotides -9 to +2 relative to a transcription start site of the gene.
  • the region includes a transcription start site of the gene.
  • the target promoter is the promoter of the target transcript. In another embodiment, the target promoter is the promoter of an isoform of the target transcript.
  • the target promoter is both the promoter of the target transcript and the promoter of an isoform of the target transcript.
  • the oligomer is selected from the group consisting of RNA,
  • DNA DNA, peptide nucleic acid, and morpholino.
  • the oligomer is a double-stranded RNA of 18-25 bases comprising a nucleotide sequence selected from the group consisting of SEQ ID NOrI-11, and 12.
  • the target transcript encodes a protein selected from the group consisting of human major vault protein (MVP), human E-cadherin, human progesterone receptor (hPR), human p53, and human PTEN.
  • MVP human major vault protein
  • hPR human progesterone receptor
  • p53 human p53
  • PTEN human PTEN
  • a method of doing business comprising promoting, marketing, selling or licensing a subject invention.
  • the invention provides methods and compositions for selectively increasing synthesis of a target transcript of a gene in a mammalian cell, wherein the target transcript is predetermined to be in need of increased synthesis, the method comprising the steps of: contacting the cell with a polynucleotide oligomer of 12-28 bases complementary to a region within a target promoter of the gene under conditions whereby the oligomer selectively increases synthesis of the target transcript; and detecting resultant selective increased synthesis of the target gene.
  • the target transcript of the gene is predetermined to be in need of increased synthesis using routine methods. For example, reduced levels of a target transcript and/or protein relative to desired levels may be directly measured. Alternatively, the need for increased synthesis of a target transcript may be inferred from a phenotype associated with reduced levels of the target transcript.
  • the region within the promoter of the gene is selected from a partially single-stranded structure, a non-B-DNA structure, an AT-rich sequence, a cruciform loop, a G-quadruplex, a nuclease hypersensitive elements (NHE), and a region located between nucleotides -100 to +25 relative to a transcription start site of the gene.
  • Preferred AT-rich sequences are found in stretches of DNA where local melting occurs, such as the promoters of genes where protein machinery must gain access to single- stranded regions, and preferably comprise the TATA box of the gene, and/or at least 60% or 70% A+T.
  • Preferred cruciform structures are formed from palindromic genomic sequences forming a hairpin structure on each strand, wherein the repeated sequences are separated by a stretch of non-pal indromic DNA providing a single-stranded loop at the end of each of the hairpins of the cruciform.
  • G-quadruplex structures are identified in promoter regions of mammalian genes and are implicated in transcription regulation.
  • nuclease hypersensitivity element III of the c-MYC oncogene promoter is involved in controlling transcription and comprises a pyrimidine-rich and purine-rich sequences on the coding and noncoding strands, respectively, that can adopt I-motif and G-quadruplex structures, respectively. Stabilization of the G-quadruplex has been shown to lead to repression of c- MYC (see e.g. Siddiqui-Jain, 2002).
  • the region targeted is located between nucleotides -100 to +25 relative to a transcription start site of the gene. In certain preferred embodiments of the method, the region is located on the template strand between nucleotides -30 to +17 relative to a transcription start site of the gene. In another embodiment, the region is located between nucleotides -15 to +10 relative to a transcription start site of the gene. In a further embodiment, the region includes nucleotides -9 to +2 relative to a transcription start site of the gene. In certain preferred embodiments, the region includes a transcription start site of the gene. In other embodiments, the region does not include any sequence downstream from the transcription start, e.g.
  • the sequence is located between nucleotides -100 to +1.
  • the oligomers used in the subject invention target genomic sequence and not mRNA. .
  • the gene is known to encode and/or express one or more isoforms of the target transcript, and the method of the invention selectively increases synthesis of the target transcript over basal expression levels and/or control condition levels, while synthesis of the isoform(s) of the target transcript may decrease, increase, or stay the same.
  • the target transcript and the isoform(s) may share the same promoter and/or transcription start site, or they may have different promoters and/or transcription start sites.
  • the target promoter is (1) the promoter of the target transcript, (2) the promoter of an isoform of the target transcript, or (3) is both the promoter of the target transcript and the promoter of an isoform of the target transcript.
  • Numerous genes are known to express multiple isoforms; examples include p53 (Bourdon, 2005), PTEN (Sharrard and Maitland, 2000), Bcl-2-related genes (Akgul, 2004), and survivin (Caldas et al, 2005).
  • the methods can be used to increase expression of one target transcript by directing oligomers to the transcription start site of an isoform.
  • the method effectively and selectively modulates relative isoform synthesis in the host cell.
  • increased synthesis of predetermined desirous or underexpressed isoforms can be coupled with decreased synthesis of predetermined undesirable or overexpressed isoforms.
  • this embodiment can be used to effect a predetermined isoform switch in the host cells.
  • the polynucleotide oligomer is of a sequence and length sufficient to effect the requisite increase of target transcript synthesis.
  • the singular forms "a,” “an,” and “the,” refer to both the singular as well as plural, unless the context clearly indicates otherwise.
  • the term “an oligomer” includes single or plural oligomers and can be considered equivalent to the phrase "at least one oligomer.”
  • Suitable oligomers are typically 12-28 bases in length, and are complementary to a region within a target promoter of the gene (i.e. Watson-Crick binding complementarity).
  • the oligomer may comprise any nucleic acid, modified nucleic acid, or nucleic acid mimic that can recognize DNA by Watson-Crick base-pairing.
  • the oligomer may be single-stranded or double- stranded (i.e. a duplex). In the case of duplex oligomers, a first strand is complementary to the region of the promoter being targeted, and the second strand is complementary to the first strand.
  • the oligomer may target homopyrimidine sequences, homopyrimidine sequences, or mixed purine/pyrimidine sequences.
  • a mixed purine/pyrimidine sequence contains at least one purine (the rest being pyrimidines) or at least one pyrimidine (the rest being purines).
  • oligomers are known in the art that are capable of Watson-Crick base-pairing.
  • the oligomer is selected from a double-stranded RNA, a DNA, a peptide nucleic acid, and a morpholino.
  • Double-stranded (ds) RNAs are particularly preferred oligomers because they are relatively easy to synthesize, and have been used in human clinical trials.
  • Preferred dsRNAs have 18-25 bases complementary to the region of the promoter being targeted, and optionally have 3' di- or trinucleotide overhangs on each strand.
  • Methods for preparing dsRNA and delivering them to cells are well-known in the art (see e.g.
  • Custom-made dsRNAs are also commercially available (e.g. Ambion Inc., Austin, TX).
  • the dsRNA used in the method of the invention may be chemically modified to enhance a desired property of the molecule. A broad spectrum of chemical modifications can be made to duplex RNA-, without negatively impacting the ability of the dsRNA to selectively increase synthesis of the target transcript.
  • the dsRNA comprises one or more nucleotides having a 2' modification, and may be entirely 2'-substituted.
  • serum stability- enhancing chemical modifications include phosphorothioate internucleotide linkages, 2'-O- methyl ribonucleotides, 2'-deoxy-2'-fiuoro ribonucleotides, 2'-deoxy ribonucleotides, "universal base” nucleotides, 5-C-methyl nucleotides, and inverted deoxyabasic residue incorporation (see e.g. US Patent Publication No. 20050032733 to McSwiggen et al).
  • the dsRNA may optionally contain locked nucleic acids (LNAs) to improve stability and increase nuclease resistance (see e.g.
  • Another type of modification is to attach a fluorescent molecule to the oligomer, for example, TAMRA, FAM, Texas Red, etc., to enable the oligomer to be tracked upon delivery to a host or to facilitate transfection efficiency determinations.
  • a fluorescent molecule for example, TAMRA, FAM, Texas Red, etc.
  • Methylase-dependent inhibition of transcription using antigene dsRNA targeting CpG islands has been described (2, 3).
  • the method of the present invention is methylase-independent, wherein synthesis of the target transcript is increased independently of, and without requiring effective methylation (e.g. transcript synthesis still occurs if the cell is contacted with the oligomer in the presence of a methylase inhibitor).
  • the target region within the target promoter is not contained within a CpG island. Algorithms for identifying CpG islands in genomic sequences are known (e.g. see Takai and Jones, 2002; and Takai and Jones 2003).
  • the oligomer is a double-stranded RNA, and the target region within the target promoter does not include a CG dinucleotide.
  • PNAs Peptide nucleic acids
  • the PNA oligomer may be homopyrimidine, optionally prepared as a bisPNA, where one PNA oligomer binds the target via Watson-Crick base pairing, and a second oligomer binds via Hoogsteen recognition (see e.g.
  • DNA oligomers can also be used in trie method of the invention. However, unmodified oligodeoxynucleotides are subject to rapid degradation by nucleases. Therefore, when DNA oligomers are used, they preferably have chemical modifications to increase nuclease resistance.
  • PS phosphorothioate
  • DNA oligomers are commercially available through numerous vendors (e.g. Integrated DNA Technologies, Coralville, IA).
  • oligomers that can be used include morpholino oligomers (see e.g. Summerton and Weller, 1997) and LNAs (see e.g. Wahlestedt et al, 2000).
  • the mammalian cell that is contacted with the oligomer can be in vitro (e.g. a cultured cell), or in situ in a host. Examples of cultured cells include primary cells, cancer cells (e.g. from cell lines), adult or embryonic stem cells, neural cells, fibroblasts, myocytes, etc.
  • the cell can be from any mammal. In one embodiment, the cell is a human cell in vitro.
  • the cell is a breast cancer cell and the gene is the human progesterone receptor.
  • the cell is a cancer cell and the gene encodes a protein selected from the group consisting of E-cadherin, human progesterone receptor (hPR), p53, and PTEN.
  • hPR human progesterone receptor
  • p53 p53
  • PTEN PTEN
  • the methods used to deliver the oligomer to the cell can vary depending on the oligomer used and whether the cell is in vitro or in vivo.
  • delivery can be accomplished by direct injection into cells.
  • delivery can be enhanced in some cases by using hydrophobic or cationic carriers i such as LipofectamineTM (Invitrogen, Carlsbad, CA).
  • the cell is a cultured cell in vitro
  • the oligomer is a double-stranded RNA of 18-25 bases
  • the cell is contacted with a composition comprising the oligomer and a cationic lipid.
  • PNA oligomers can be introduced into cells in vitro by complexing them with partially complementary DNA oligonucleotides and cationic lipid (21-25). The lipid promotes internalization of the DNA, while the PNA enters as cargo and is subsequently released. Peptides such as penetratin, transportan, Tat peptide, nuclear localization signal (NLS), and others, can be attached to the oligomer to promote cellular uptake (see e.g., Nielsen, 2004; Kaihatsu et al, 2003; Kaihatsu, et al, 2004; and ref. 7). Alternatively, the cells can be permeabilized with a permeabilization agent such as lysolecithin, and then contacted with the oligomer.
  • a permeabilization agent such as lysolecithin
  • Viral transduction can be used to deliver oligomers to cells in vitro (e.g. lentiviral transduction, see e.g. ref 7).
  • the contacting step is free of viral transduction.
  • the contacting step is free of viral transduction, and the oligomer is not attached to a nuclear localization peptide.
  • compositions consisting essentially of the oligomer (in a carrier solution) can be directly injected into the host (see e.g. Tyler et al, 1999; McMahon et al, 2002).
  • the cell is in situ in a host, the oligomer is a double-stranded RNA of 18-25 bases, and the cell' is contacted with a composition consisting essentially of the oligomer.
  • the methods of the invention provide at least a 1.2-fold resultant increased synthesis of the target transcript relative to control conditions and/or basal expression levels. In other embodiments, increases of at least 1.5, 1.7, 2.0, 2.5, 3.0, 3.5, or 4.0 fold are achieved. Efficient increased synthesis of the target transcript can be achieved without viral transduction; in fact, in preferred embodiments the contacting step is free of viral transduction. While multiple regions of the target promoter can be targeted, highly efficient increased synthesis of the target transcript can be achieved with dsRNA targeting just a single region of the target promoter.
  • the oligomer is a dsRNA of 18-25 bases, there is at least a 2-fold resultant increased synthesis of the target transcript relative to control conditions and/or basal expression levels, and a single region of the target promoter is targeted.
  • Significant increases in synthesis of the target transcript can be achieved using nanomolar or picomolar (submicromolar) concentrations of the oligomer, and it is typically preferred to use the lowest concentration possible to achieve the desired resultant increased synthesis, e.g. oligomer concentrations in the 1-10OnM range are preferred; more preferably, the concentration is in the 1-5OnM, l-25nM, 1-1OnM, or picomolar range.
  • detecting step of the method selective increased synthesis of the target transcript resulting from the oligomer contacting the cell is detected. This can be determined directly by measuring an increase in the level of the gene's mRNA transcript, or indirectly by detecting increased levels of the corresponding encoded protein compared to controls. Alternatively, resultant selective increased synthesis of the target transcript may be inferred based on phenotypic changes that are indicative of increased synthesis of the target transcript.
  • polynucleotide oligomers for selectively increasing synthesis of a target transcript of a gene, the oligomer comprising a nucleotide sequence of 12-28 bases complementary to a region within a target promoter of the gene located between nucleotides -100 to +25 relative to a transcription start site of the gene.
  • the oligomer selectively increases transcription of the target transcript.
  • the target transcript encodes a protein selected from the group consisting of human major vault protein (MVP), human E- cadherin, human progesterone receptor (hPR), human p53, and human PTEN.
  • the oligomer is RNA, DNA, peptide nucleic acid or morpholino.
  • the nucleic acid oligomer is a dsRNA of 18-25 bases.
  • Specific gene targets and dsRNA sequences that selectively increase transcript synthesis are listed in Table 1. Only one strand (shown 5' to 3') of each dsRNA is shown. Additionally the dsRNAs had 3'-dithymidine overhangs on each strand. [057]
  • the invention provides a method of doing business comprising promoting, marketing, selling or licensing any of the aforementioned inventions.
  • Example 1 agRNA-induced transcriptional increase
  • MVP Using previously described methods (Janowski et al., 2005), dsRNAs targeting the major vault protein (MVP; Lange et al, 2000) at -82 to -64 (-82 ⁇ 64) relative to the transcription start site, and the p53 site ( ⁇ 54/-36), caused 2.9 and 3.8 fold increases respectively in MVP expression at the level of RNA and protein.
  • MVP major vault protein
  • p53 site ⁇ 54/-36
  • E-Cadherin An RNA targeting the -10/+9 region of E-cadherin (referred to as EC9), caused a 1.5-2.1 increase in E-cadherin expression at the level of RNA and protein. RNAs targeting -9/+10, -13/+6 and -14/+5 caused no increase or inhibited expression and gene activation. Activation of E-cadherin expression by EC9 was observed in three independent experiments.
  • h-PR We have tested twenty-one RNAs complementary to progesterone receptor
  • EXAMPLE 2 Increased human progesterone receptor (hPR) expression by antigene PNA (agPNA) oligomers targeting near the transcription start site
  • hPR human progesterone receptor
  • agPNA antigene PNA
  • oligomers targeting near the transcription start site [068J Cell Culture T47D breast cancer cells (American Type Cell Culture Collection, ATCC) are maintained at 37°C and 5% CO 2 in RPMI media (ATCC) supplemented with 10% (v/v) heat-inactivated (56°C, 1 hr) fetal bovine serum (Gemini Bioproducts), 0.5% nonessential amino acids (Sigma), 0.4 units/mL bovine insulin (Sigma) and 100 units/ml penicillin and 0.1 mg/ml streptomycin (Sigma).
  • RNA Analysis Total RNA from treated T47D cells is extracted using trizol (TRIzol, Invitrogen). RNA is treated with deoxyribonuclease to remove contaminating DNA, and 4 ⁇ g are reverse transcribed by random primers using Superscript II RNase H-reverse transcriptase (Invitrogen).
  • Microscopy Cells are imaged by confocal microscopy using a Zeiss Axiovert 200 M inverted transmitted light microscope (Carl Zeiss Microimaging). Approximations of cell height are made by tracking distances in the Z-plane using an automated program. Individual cells are chosen for observation and then the microscope is underfocused until no part of the individual cell is in focus.
  • Example 3 VEGF-Activating agRNAs Increase Vascularization
  • the promoter region of the human VEGF gene has been characterized (see e.g. Tischer et al, 1991).
  • the transcription start site is at position 2363 in the published sequence (GenBank Accession no. AF095785.1).
  • 19-mer agRNAs fully complementary to the template strand and targeting near the transcription start site of the gene (-50 to +25, where transcription start is +1) are prepared; exemplary agRNAs are shown in Table 4 (second strand and dinucleotide overhangs not shown).
  • Table 4 Table 4
  • agRNAs The effect of the agRNAs on selectively increasing synthesis of VEGF transcripts is determined in primary human umbilical vein cells (HUVECs). Resultant selective increased synthesis of the VEGF transcript is detected inferentially from increases in cell proliferation and/or directly by measuring increases in VEGF gene transcripts relative to controls. agRNAs resulting in at least a 2-fold increase in VEGF gene transcription are evaluated in animal model and clinical studies for treatment of myocardial ischemia as described below. [077] Ischemic Heart Model Adenoviral vectors are constructed for delivery of VEGF- activating agRNAs to an ischemic heart mouse model using known methods (see e.g.
  • CDl mice (Charles River Breeding Laboratories) are anesthetized with 15-16 ⁇ l of 2.5% Avertin per gram of body weight by i.p. injection. After the respiration of the animal is controlled by a Small Animal Volume Controlled Ventilator (Harvard Rodent Ventilator, model 683, South Natick, MA), a thoracotomy incision is made in the fourth intercostal space. A surgical retractor is put in the incision to expose the heart. The anterior descending coronary artery is ligated permanently with a 6-0 nonabsorbable surgical suture to induce ischemia.
  • IxIO 1 genomes of viral vectors in 50 ⁇ l of Hepes saline (pH 7.4) is injected directly to multiple sites of the myocardium on the left ventricle wall around the ischemic region. Control mice receive buffer injections. Cardiac function is assessed 4 weeks after the surgery. Left ventricular end diastolic dimension (LVDd) and end systolic dimension (LVDs) are measured. The percentage of fractional shortening (FS%) is calculated as (LVDd - LVDs) / LVDd x 100. [078] Hearts collected after echocardiography are sectioned and stained with anti-platelet endothelial cell adhesion molecule 1 and smooth muscle ⁇ -actin antibodies.
  • Vessels are counted on six areas, three on the anterior wall and three on the posterior wall in cross sections of the left ventricle. Area 1 is made up entirely of muscle tissue, area 2 has both muscle and scar, and area 3 has scar only. Vectors are injected into area 2 at the anterior wall. Hence, comparison between the injected areas in the anterior and the corresponding uninjected posterior areas indicates the effect of the agRNA on VEGF expression.
  • Capillary density is expressed as the ratio of capillary to cardiac myocyte for area 1 and as the number of capillaries per mm 2 for areas 2 and 3.
  • the density of ⁇ -actin-positive vessels is expressed as the number of vessels per mm 2 or all areas. Activation of VEGF expression is demonstrated by an increase in capillaries and ⁇ -actin-positive vessels in all three areas of the anterior walls compared with the posterior walls in the same hearts and compared with the anterior walls of control groups.
  • Eligible patients include Canadian Cardiovascular Society (CCS) class III or IV angina refractory to maximum medical therapy, multivessel coronary artery disease not suitable for bypass surgery or angioplasty, and reversible ischemia on stress SPECT Tc 99m sestamibi nuclear imaging.
  • Subjects are excluded if they had a previous history or current evidence of malignancy, active diabetic retinopathy, or evidence of severe LV systolic dysfunction (LV ejection fraction [EF] ⁇ 20% by transthoracic 2D echocardiography).
  • VEGF-inducing agRNA-expressing vectors are injected into the patients. Subjects undergo nonfluoroscopic LV EMM immediately before injection of the vector to guide injections to foci of ischemic myocardium. Follow-up EMM is performed at 12 weeks after injections. The pre-specified primary efficacy parameters are change from baseline in CCS angina classification and exercise tolerance at the 12-week follow-up visit.
  • Example 4 agRNA-induced transcriptional increase
  • RNA PRl 1 a duplex complementary to the PR promoter sequence from -11 to +8. Wc chose PRl 1 because it had not inhibited PR expression in T47D cells but was surrounded by agRNAs that were potent inhibitors. For comparison, we also tested RNAs PR9 and PR26 that we had previously shown to be potent inhibitors of PR expression in T47D cells.
  • RNA PRl 1 into MCF-7 cells using cationic lipid (Janowski et al (2006b) Nature Protocols 1 :436-443) and observed an 18-fold increase in levels of PR protein by Western analysis, indicating that agRNAs could produce substantial up-regulation of gene expression when tested in an appropriate cellular context.
  • Addition of PR9 did not affect PR expression, while PR26 yielded a modest 2-fold increase in PR levels.
  • Two siRNAs that were complementary to downstream coding sequences within PR mRNA inhibited expression of PR protein, demonstrating that PR levels could be reduced by standard post- transcriptional silencing in MCF-7 cells.
  • PR protein is expressed as two isoforms, PR-A and PR-B, which play differing roles in physiologic processes (Conneely et al, (2003) Mammary Gland Biol. Neoplasia 8:205-214).
  • the promoter for PR-B is upstream from the promoter for PR-A and the RNAs used in this study target the PR-B promoter.
  • RNAs targeting the PR-B promoter can also enhance expression of both PR-B and PR-A protein, providing complementary evidence that expression of the isoforms is linked.
  • duplex RNAs targeted to sequences throughout the region -56 to +17 within the PR promoter.
  • Several of these duplex RNAs induced expression of PR by 5 fold or greater (Table 5).
  • Small shifts in target sequence had large consequences for activation.
  • PR12 single base shift upstream
  • PRlO downstream
  • RNAs targeted to major vault protein MVP
  • MVP major vault protein
  • MVP6 and MVP9 inhibited gene expression, a result that we had reported previously (Janowski et al, 2005).
  • MVP35 corresponding to nucleotides 1819-1837 of Genbank Accession no. AJ238509, GL583487
  • MVP54, and MVP82 increased expression by 2-4 fold above normal levels.
  • Quantitative PCR reveals that treatment of MCF-7 cells with PRl 1 enhances expression of PR mRNA under a variety of cell culture conditions.
  • siRNAs Hardy et al (2006) MoI Endocrinol, Epub ahead of print June 13, 2006
  • agRNAs unpublished significantly increases expression of cyclooxygenase-2 (COX-2) after induction with interleukinl beta (IL- l ⁇ ).
  • COX-2 cyclooxygenase-2
  • IL- l ⁇ interleukinl beta
  • a specific embodiment of our invention is a method for decreasing Cox-2 expression in a cell by contacting the cell with a polynucleotide oligomer of 12-28 bases complementary to a region located between nucleotides -100 to +25 relative to a transcription start site of the human progesterone receptor (hPR) gene under conditions whereby the oligomer selectively increases synthesis of the hPR; and detecting decreased synthesis of the Cox-2; wherein the oligomer is preferably double-stranded RNA.
  • hPR human progesterone receptor

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Publication number Priority date Publication date Assignee Title
WO2009099942A3 (en) * 2008-01-31 2009-10-15 Alnylam Pharmaceuticals, Inc. Chemically modified oligonucleotides and uses thereof
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US12084659B2 (en) 2016-05-18 2024-09-10 Voyager Therapeutics, Inc. Modulatory polynucleotides

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JP2013523162A (ja) 2010-04-06 2013-06-17 アルナイラム ファーマシューティカルズ， インコーポレイテッド Ｃｄ２７４／ｐｄ−ｌ１遺伝子の発現を阻害するための組成物および方法
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US8895528B2 (en) 2010-05-26 2014-11-25 Curna, Inc. Treatment of atonal homolog 1 (ATOH1) related diseases by inhibition of natural antisense transcript to ATOH1
US8980858B2 (en) 2010-05-26 2015-03-17 Curna, Inc. Treatment of methionine sulfoxide reductase a (MSRA) related diseases by inhibition of natural antisense transcript to MSRA
KR101914309B1 (ko) 2010-06-23 2018-11-02 큐알엔에이, 인크. 전압 작동 나트륨 통로, 알파 소단위(scna)에 대한 자연 안티센스 전사체의 저해에 의한 전압 작동 나트륨 통로, 알파 소단위(scna) 관련된 질환의 치료
JP5998131B2 (ja) 2010-07-14 2016-09-28 カッパーアールエヌエー，インコーポレイテッド Ｄｉｓｃｓｌａｒｇｅｈｏｍｏｌｏｇ（ｄｌｇ）ｄｌｇ１への天然アンチセンス転写物の阻害によるｄｌｇ関連疾患の治療
KR101886457B1 (ko) 2010-10-06 2018-08-07 큐알엔에이, 인크. 시알리다아제 4 (neu4)에 대한 자연 안티센스 전사체의 저해에 의한 neu4 관련된 질환의 치료
WO2012054723A2 (en) 2010-10-22 2012-04-26 Opko Curna Llc Treatment of alpha-l-iduronidase (idua) related diseases by inhibition of natural antisense transcript to idua
US10000752B2 (en) 2010-11-18 2018-06-19 Curna, Inc. Antagonat compositions and methods of use
ES2657590T3 (es) 2010-11-23 2018-03-06 Curna, Inc. Tratamiento de enfermedades relacionadas con nanog mediante inhibición del transcrito antisentido natural a nanog
WO2012078967A2 (en) 2010-12-10 2012-06-14 Alnylam Pharmaceuticals, Inc. Compositions and methods for increasing erythropoietin (epo) production
WO2012079046A2 (en) 2010-12-10 2012-06-14 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of klf-1 and bcl11a genes
EP2508530A1 (en) 2011-03-28 2012-10-10 Rheinische Friedrich-Wilhelms-Universität Bonn Purification of triphosphorylated oligonucleotides using capture tags
CA3217805A1 (en) 2011-03-29 2012-10-04 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of tmprss6 gene
TWI678375B (zh) 2011-06-09 2019-12-01 可娜公司 藉由抑制共濟蛋白（ｆｒａｔａｘｉｎ，ｆｘｎ）之天然反股轉錄本治療ｆｘｎ相關疾病
WO2012177906A1 (en) 2011-06-21 2012-12-27 Alnylam Pharmaceuticals, Inc. Assays and methods for determining activity of a therapeutic agent in a subject
CN103874486A (zh) 2011-09-06 2014-06-18 库尔纳公司 用小分子治疗与电压门控钠通道的α亚基(SCNxA)相关的疾病
EP2825648B1 (en) 2012-03-15 2018-09-05 CuRNA, Inc. Treatment of brain derived neurotrophic factor (bdnf) related diseases by inhibition of natural antisense transcript to bdnf
US9133461B2 (en) 2012-04-10 2015-09-15 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the ALAS1 gene
CN104583398A (zh) 2012-05-16 2015-04-29 Rana医疗有限公司 用于调节基因表达的组合物和方法
EP2712870A1 (en) 2012-09-27 2014-04-02 Rheinische Friedrich-Wilhelms-Universität Bonn Novel RIG-I ligands and methods for producing them
JP2016528897A (ja) * 2013-08-16 2016-09-23 ラナ セラピューティクス インコーポレイテッド Ｒｎａを調節するための組成物および方法
TWI669393B (zh) 2013-10-02 2019-08-21 艾爾妮蘭製藥公司 抑制ｌｅｃｔ２基因表現之組合物及方法
LT3052628T (lt) 2013-10-04 2020-09-10 Alnylam Pharmaceuticals, Inc. Kompozicijos ir būdai alas1 geno raiškai nuslopinti
WO2016130943A1 (en) 2015-02-13 2016-08-18 Rana Therapeutics, Inc. Hybrid oligonucleotides and uses thereof
WO2016170348A2 (en) 2015-04-22 2016-10-27 Mina Therapeutics Limited Sarna compositions and methods of use
JP2018538302A (ja) * 2015-12-15 2018-12-27 サレプタ セラピューティクス， インコーポレイテッド ペプチドオリゴヌクレオチド複合体
WO2019048631A1 (en) 2017-09-08 2019-03-14 Mina Therapeutics Limited SMALL HNF4A ACTIVATOR RNA COMPOSITIONS AND METHODS OF USE
US20200208152A1 (en) 2017-09-08 2020-07-02 Mina Therapeutics Limited Stabilized sarna compositions and methods of use
MX2020004507A (es) 2017-11-09 2020-08-13 Alnylam Pharmaceuticals Inc Ensayos y métodos para determinar la expresión del gen de quimiotaxina 2 derivada de leucocitos (lect2).
US11566246B2 (en) 2018-04-12 2023-01-31 Mina Therapeutics Limited SIRT1-saRNA compositions and methods of use
AU2019285344A1 (en) 2018-06-15 2020-12-10 Mina Therapeutics Limited Combination therapies comprising C/EBP alpha saRNA
EP3837367A1 (en) 2018-08-16 2021-06-23 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the lect2 gene
US20220211740A1 (en) 2019-04-12 2022-07-07 Mina Therapeutics Limited Sirt1-sarna compositions and methods of use
JP2022545101A (ja) 2019-08-19 2022-10-25 ミナ セラピューティクス リミテッド オリゴヌクレオチドコンジュゲート組成物および使用方法
US20220290152A1 (en) 2019-09-03 2022-09-15 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the lect2 gene
AU2020349991A1 (en) * 2019-09-20 2022-04-07 Ractigen Therapeutics Nucleic acid molecule for treating immune thrombocytopenia and application thereof
EP4038189A1 (en) 2019-10-04 2022-08-10 Alnylam Pharmaceuticals, Inc. Compositions and methods for silencing ugt1a1 gene expression
CA3170377A1 (en) 2020-02-10 2021-08-19 Alnylam Pharmaceuticals, Inc. Compositions and methods for silencing vegf-a expression
EP4127171A2 (en) 2020-03-30 2023-02-08 Alnylam Pharmaceuticals, Inc. Compositions and methods for silencing dnajc15 gene expression
US20230295622A1 (en) 2020-04-06 2023-09-21 Alnylam Pharmaceuticals, Inc. Compositions and methods for silencing myoc expression
US20230159933A1 (en) 2020-04-07 2023-05-25 Alnylam Pharmaceuticals, Inc. Compositions and methods for silencing scn9a expression
US20230183707A1 (en) 2020-05-21 2023-06-15 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting marc1 gene expression
JP2024511092A (ja) 2021-03-26 2024-03-12 ミナ セラピューティクス リミテッド ＴＭＥＭ１７３ｓａＲＮＡ組成物及び使用方法
WO2022229644A1 (en) 2021-04-28 2022-11-03 Mina Therapeutics Limited Combination therapies comprising c/ebp alpha sarna
EP4395790A2 (en) 2021-08-31 2024-07-10 Alnylam Pharmaceuticals, Inc. Cell death-inducing dffa-like effector b (cideb) irna compositions and methods of use thereof
WO2023099884A1 (en) 2021-12-01 2023-06-08 Mina Therapeutics Limited Pax6 sarna compositions and methods of use
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WO2024134199A1 (en) 2022-12-22 2024-06-27 Mina Therapeutics Limited Chemically modified sarna compositions and methods of use
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WO2025224036A1 (en) 2024-04-22 2025-10-30 Mina Therapeutics Limited Chemically modified sarna compositions and methods of use

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5859221A (en) 1990-01-11 1999-01-12 Isis Pharmaceuticals, Inc. 2'-modified oligonucleotides
WO2001007164A1 (en) 1999-07-27 2001-02-01 Hitachi Chemical Co., Ltd. Filtration vacuum pad
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
US6673611B2 (en) 1998-04-20 2004-01-06 Sirna Therapeutics, Inc. Nucleic acid molecules with novel chemical compositions capable of modulating gene expression
US20050032733A1 (en) 2001-05-18 2005-02-10 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (SiNA)

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5877160A (en) * 1991-05-31 1999-03-02 Genta Incorporated Compositions and methods of treatment of androgen-associated baldness using antisense oligomers
WO2003070918A2 (en) * 2002-02-20 2003-08-28 Ribozyme Pharmaceuticals, Incorporated Rna interference by modified short interfering nucleic acid
WO2002081628A2 (en) * 2001-04-05 2002-10-17 Ribozyme Pharmaceuticals, Incorporated Modulation of gene expression associated with inflammation proliferation and neurite outgrowth, using nucleic acid based technologies
KR20080023768A (ko) 2000-03-30 2008-03-14 화이트헤드 인스티튜트 포 바이오메디칼 리서치 Ｒｎａ 간섭의 ｒｎａ 서열 특이적인 매개체
ATE353361T1 (de) 2000-04-28 2007-02-15 Sangamo Biosciences Inc Gezielten modifikation der chromatinstruktur
US6867349B2 (en) * 2000-07-31 2005-03-15 Regents Of The University Of Minnesota Inhibition of gene expression using polynucleotide analogues
KR100909681B1 (ko) * 2000-12-01 2009-07-29 막스-플랑크-게젤샤프트 츄어 푀르더룽 데어 비쎈샤프텐 에.파우. Ｒｎａ 간섭을 매개하는 작은 ｒｎａ 분자
DK1355919T3 (da) 2000-12-12 2011-03-14 Medimmune Llc Molekyler med længere halveringstider, sammensætninger og anvendelser deraf
US20050148530A1 (en) * 2002-02-20 2005-07-07 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
CN1318586C (zh) * 2001-11-05 2007-05-30 詹森药业有限公司 短的双链RNAs的体外合成方法
WO2003044214A2 (en) * 2001-11-20 2003-05-30 Baylor College Of Medicine Methods and compositions in checkpoint signaling
US8999943B2 (en) * 2005-03-14 2015-04-07 Board Of Regents, The University Of Texas System Antigene oligomers inhibit transcription
US8877721B2 (en) 2005-04-15 2014-11-04 The Regents Of The University Of California Small activating RNA molecules and methods of use
JP5066095B2 (ja) 2005-11-17 2012-11-07 ボード・オブ・リージエンツ，ザ・ユニバーシテイ・オブ・テキサス・システム 染色体ｄｎａに標的化されるオリゴマーによる遺伝子発現の調節

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5859221A (en) 1990-01-11 1999-01-12 Isis Pharmaceuticals, Inc. 2'-modified oligonucleotides
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
US6673611B2 (en) 1998-04-20 2004-01-06 Sirna Therapeutics, Inc. Nucleic acid molecules with novel chemical compositions capable of modulating gene expression
WO2001007164A1 (en) 1999-07-27 2001-02-01 Hitachi Chemical Co., Ltd. Filtration vacuum pad
US20050032733A1 (en) 2001-05-18 2005-02-10 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (SiNA)

Non-Patent Citations (59)

* Cited by examiner, † Cited by third party
Title
AKGUL ET AL., CELL. MOL. LIFE SCI., vol. 61, 2004, pages 2189 - 2199
BERNSTEIN; ALLIS, GENES DEV., vol. 19, 2005, pages 1635 - 1655
BOURDON ET AL., GENES DEV, vol. 19, 2005, pages 2122 - 2137
BRAASCH ET AL., BIOCHEMISTRY, vol. 42, 2003, pages 7967 - 75
CALDAS ET AL., ONCOGENE, vol. 24, 2005, pages 1994 - 2007
CASTANOTTO ET AL., MOL. THERAPY, vol. 12, 2005, pages 179 - 183
CONNEELY ET AL., MAMMARY GLAND BIOL. NEOPLASIA, vol. 8, 2003, pages 205 - 214
CZAUDEMA ET AL., NUCLEIC ACIDS RES., vol. 31, 2003, pages 2705 - 16
ELBASHIR ET AL., NATURE, vol. 411, 2001, pages 494 - 8
ELMEN ET AL., NUCLEIC ACIDS RES., vol. 33, 2005, pages 439 - 47
GREWAL; MOAZED, SCIENCE, vol. 301, 2003, pages 798 - 802
HAAIMA ET AL., NUCLEIC ACIDS RES., vol. 25, 1997, pages 4639 - 43
HAHN, NAT STRUCT MOL BIOL.L, vol. 11, 2004, pages 394 - 403
HARDY ET AL., MOL ENDOCRINOL, 13 June 2006 (2006-06-13)
HASSANI ET AL., J GENE MED., vol. 7, 2004, pages 198 - 207
HUFFMAN; COREY, BIOCHEMISTRY, vol. 44, 2004, pages 2253 - 2261
HURD ET AL., J. BIOL. CHEM, 1995
JANOWSKI ET AL., NATURE CHEM BIOL, vol. 1, 2005, pages 210 - 216
JANOWSKI ET AL., NATURE CHEM BIOL, vol. 1, 2005, pages 216 - 222
JANOWSKI ET AL., NATURE CHEM. BIOL, vol. 1, 2006, pages 210 - 215
JANOWSKI ET AL., NATURE PROTOCOLS, vol. 1, 2006, pages 436 - 443
JANOWSKI ET AL., NATURE STRUC. MOL. BIOL, vol. 13, 2006, pages 787 - 792
JENSTER ET AL., PROC NATL ACAD SCI USA, vol. 94, 1997, pages 7879 - 7884
KAIHATSU ET AL., BIOCHEMISTRY, vol. 42, 2003, pages 13996 - 4003
KAIHATSU ET AL., BIOCHEMISTRY, vol. 43, 2004, pages 14340 - 14347
KAWASAKI; TAIRA, NATURE, vol. 431, 2004, pages 211 - 7
KUWABARA ET AL., CELL, vol. 116, 2004, pages 779 - 793
KUWABARA ET AL., NUC ACID SYMP SERIES, vol. 49, 2005, pages 87 - 88
LAMB; CRAWFORD, MOL CELL BIOL, vol. 6, 1986, pages 1379 - 1385
LANGE ET AL., BIOCHEM BIOPHYS RES COMM, vol. 278, 2000, pages 125 - 133
LI ET AL., PROC AMER ASSOC CANCER RES, vol. 46, 2005, pages 6105
MASSENEGGER ET AL., CELL, vol. 76, 1994, pages 567 - 576
MATZKE ET AL., BIOCHEM. BIOPHYS. ACTA, vol. 1677, 2004, pages 129 - 141
MORRIS ET AL., SCIENCE, vol. 305, 2004, pages 1289 - 92
NIELSEN, MOL BIOTECHNOL., vol. 26, 2004, pages 233 - 48
PARK ET AL., BIOCHEM. BIOPHYS. RES. COMM., vol. 323, 2004, pages 275 - 280
PAROO; COREY, TRENDS BIOTECHNOL., vol. 22, 2004, pages 390 - 4
PELISSIER; WASSENEGGER, RNA, vol. 6, 2000, pages 55 - 65
ROHALY, CELL, vol. 122, 2005, pages 21 - 32
SCHRAMKE ET AL., NATURE, vol. 435, 2005, pages 1275 - 1279
See also references of EP1957648A4
SHARRARD; MAITLAND, BIOCHIM BIOPHYS ACTA, vol. 1494, 2000, pages 282 - 285
SIGOVA ET AL., GENES DEV., vol. 18, 2004, pages 2359 - 2367
SU ET AL., PROC. NATL. ACAD. SCI USA, vol. 101, 2004, pages 16280 - 16285
SU ET AL., PROC. NATL. ACAD. SCI USA, vol. 99, 2002, pages 9480 - 9485
SUGIYAMA ET AL., PROC. NATL. ACAD. SCI. USA, vol. 102, 2005, pages 152 - 157
SUMMERTON; WELLER, ANTISENSE NUCLEIC ACID DRUG DEV., vol. 7, 1997, pages 187 - 95
SVOBODA, P. ET AL., NUCL. ACIDS. RES., vol. 32, 2004, pages 3601 - 3606
TAKAI; JONES, IN SILICO BIOL., vol. 3, 2003, pages 235 - 40
TAKAI; JONES, PROC NATL ACAD SCI USA., vol. 99, 2002, pages 3740 - 5
TANG, TRENDS BIOCHEM. SCI., vol. 30, 2004, pages 106 - 114
TING ET AL., NAT GENET., vol. 37, 2005, pages 906 - 10
TISCHER ET AL., J BIOL CHEM., vol. 266, 1991, pages 11947 - 54
TYLER ET AL., PNAS, vol. 96, 1999, pages 7053 - 7058
URBAN-KLEIN ET AL., GENE THER., vol. 12, 2005, pages 461 - 6
VERDEL ET AL., SCIENCE, vol. 303, 2003, pages 672 - 676
VOLPE ET AL., SCIENCE, vol. 297, 2002, pages 1833 - 1837
WAHLESTEDT ET AL., PROC. NATL ACAD. SCI. USA, vol. 97, 2000, pages 5633 - 5638
ZENDER ET AL., PROC. NATL. ACAD. SCI USA, vol. 100, 2003, pages 7797 - 802

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8999943B2 (en) 2005-03-14 2015-04-07 Board Of Regents, The University Of Texas System Antigene oligomers inhibit transcription
US8324181B2 (en) 2005-11-17 2012-12-04 Board Of Regents, The University Of Texas System Modulation of gene expression by oligomers targeted to chromosomal DNA
EP2205746A4 (en) * 2007-10-04 2010-12-22 Univ Texas MODULATION OF GENE EXPRESSION WITH AGRNA AND GAPS WITH ANTISENSE TRANSCRIPTS AS A TARGET
JP2010539990A (ja) * 2007-10-04 2010-12-24 ボード オブ リージェンツ ザ ユニバーシティー オブ テキサス システム アンチセンス転写物を標的とするａｇＲＮＡおよびギャップマーを用いた遺伝子発現の調節方法
WO2009099942A3 (en) * 2008-01-31 2009-10-15 Alnylam Pharmaceuticals, Inc. Chemically modified oligonucleotides and uses thereof
US8222221B2 (en) 2008-06-04 2012-07-17 The Board Of Regents Of The University Of Texas System Modulation of gene expression through endogenous small RNA targeting of gene promoters
WO2010121956A1 (en) 2009-04-21 2010-10-28 Basf Plant Science Company Gmbh Rna-mediated induction of gene expression in plants
WO2010121952A1 (en) 2009-04-21 2010-10-28 Basf Plant Science Company Gmbh Rna-mediated induction of gene expression in plants
DE112010001703T5 (de) 2009-04-21 2012-09-20 Basf Plant Science Company Gmbh RNA-vermittelte Induktion von Genexpression in Pflanzen
DE112010001705T5 (de) 2009-04-21 2013-01-24 Basf Plant Science Company Gmbh RNA-vermittelte lnduktion von Genexpression in Pflanzen
US8916534B2 (en) 2010-10-08 2014-12-23 Mina Therapeutics Limited Methods of inducing insulin production
US9885046B2 (en) 2010-10-08 2018-02-06 Mina Therapeutics Limited Methods of inducing insulin production
US11365414B2 (en) 2010-10-08 2022-06-21 Mina Therapeutics Limited Methods of inducing insulin production
US8835400B2 (en) 2010-10-08 2014-09-16 Mina Therapeutics Limited RNA molecules that upregulate insulin production
US10774331B2 (en) 2010-10-08 2020-09-15 Mina Therapeutics Limited Methods of inducing insulin production
US10358644B2 (en) 2010-11-12 2019-07-23 The General Hospital Corporation Polycomb-associated non-coding RNAs
US9816094B2 (en) 2010-11-12 2017-11-14 The General Hospital Corporation Polycomb-associated non-coding RNAs
US9856479B2 (en) 2010-11-12 2018-01-02 The General Hospital Corporation Polycomb-associated non-coding RNAs
US9920317B2 (en) 2010-11-12 2018-03-20 The General Hospital Corporation Polycomb-associated non-coding RNAs
US10053694B2 (en) 2010-11-12 2018-08-21 The General Hospital Corporation Polycomb-associated non-coding RNAS
US10119144B2 (en) 2010-11-12 2018-11-06 The General Hospital Corporation Polycomb-associated non-coding RNAs
US11066673B2 (en) 2010-11-12 2021-07-20 The General Hospital Corporation Polycomb-associated non-coding RNAs
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US10059941B2 (en) 2012-05-16 2018-08-28 Translate Bio Ma, Inc. Compositions and methods for modulating SMN gene family expression
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US10655128B2 (en) 2012-05-16 2020-05-19 Translate Bio Ma, Inc. Compositions and methods for modulating MECP2 expression
US10174323B2 (en) 2012-05-16 2019-01-08 The General Hospital Corporation Compositions and methods for modulating ATP2A2 expression
US10837014B2 (en) 2012-05-16 2020-11-17 Translate Bio Ma, Inc. Compositions and methods for modulating SMN gene family expression
US10174315B2 (en) 2012-05-16 2019-01-08 The General Hospital Corporation Compositions and methods for modulating hemoglobin gene family expression
US10202601B2 (en) 2013-11-22 2019-02-12 Mina Therapeutics Limited C/EBPα short activating RNA compositions and methods of use
US10633659B2 (en) 2013-11-22 2020-04-28 Mina Therapeutics Limited C/EBPα short activating RNA compositions and methods of use
US10858650B2 (en) 2014-10-30 2020-12-08 The General Hospital Corporation Methods for modulating ATRX-dependent gene repression
EP3907287A1 (en) 2014-11-14 2021-11-10 Voyager Therapeutics, Inc. Modulatory polynucleotides
US12071625B2 (en) 2014-11-14 2024-08-27 Voyager Therapeutics, Inc. Modulatory polynucleotides
US10900036B2 (en) 2015-03-17 2021-01-26 The General Hospital Corporation RNA interactome of polycomb repressive complex 1 (PRC1)
US12084659B2 (en) 2016-05-18 2024-09-10 Voyager Therapeutics, Inc. Modulatory polynucleotides

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