WO2007049988A1 - Procede de fabrication de materiaux biologiques a partir de tissus osseux et materiau d'osteoplastie et d'ingenierie de tissus obtenu au moyen de ce procede - Google Patents

Procede de fabrication de materiaux biologiques a partir de tissus osseux et materiau d'osteoplastie et d'ingenierie de tissus obtenu au moyen de ce procede Download PDF

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Publication number
WO2007049988A1
WO2007049988A1 PCT/RU2005/000526 RU2005000526W WO2007049988A1 WO 2007049988 A1 WO2007049988 A1 WO 2007049988A1 RU 2005000526 W RU2005000526 W RU 2005000526W WO 2007049988 A1 WO2007049988 A1 WO 2007049988A1
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WO
WIPO (PCT)
Prior art keywords
bone
hours
washing
collagen
plates
Prior art date
Application number
PCT/RU2005/000526
Other languages
English (en)
French (fr)
Inventor
Andrey Fedorovich Panasyuk
Original Assignee
Savaschuk, Dmitry Alekseevich
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Savaschuk, Dmitry Alekseevich filed Critical Savaschuk, Dmitry Alekseevich
Priority to US12/091,741 priority Critical patent/US8241673B2/en
Priority to EP05857392A priority patent/EP1941916B1/en
Priority to PCT/RU2005/000526 priority patent/WO2007049988A1/ru
Publication of WO2007049988A1 publication Critical patent/WO2007049988A1/ru
Priority to US13/572,793 priority patent/US8685092B2/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
    • A61L27/3645—Connective tissue
    • A61L27/365—Bones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/24—Collagen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/3608—Bone, e.g. demineralised bone matrix [DBM], bone powder
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3691—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
    • A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
    • A61L27/446—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix with other specific inorganic fillers other than those covered by A61L27/443 or A61L27/46
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Epidemiology (AREA)
  • Dermatology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Molecular Biology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Vascular Medicine (AREA)
  • Biophysics (AREA)
  • Inorganic Chemistry (AREA)
  • Composite Materials (AREA)
  • Materials Engineering (AREA)
  • Rheumatology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Claims

Формула изобретения
1. Способ получения биоматериалов из костной ткани, заключающийся в очистке кости природного происхождения, распиливании ее на фрагменты заданной толщины, удалении липидов из природной костной ткани, экстракцию с последующей промывкой и лиофилизацией конечного продукта, отличающийся тем, что после очистки кость природного происхождения распиливают на пластины толщиной от 0,2 до 2,0 см, двухкратно отмывают нагретым до 65°C 0,1 M фосфатным буфером - рН 5,8-6,0 из расчета на одну часть кости два объема буферного раствора, переваривают в растворе активированного 0,1-0,4 % папаина при 650C в течение 24 часов, затем пластины промывают 5-ю объемами воды при 40 - 800C, обрабатывают раствором 0,4 N щелочи при комнатной температуре в течение 10-24 часов, отмывают проточной водой, высушивают, обезжиривают в смесях этанол/хлороформ сначала в соотношении 1 :2, а затем в соотношении 2:1 из расчета два объема смеси на одну часть кости и каждый раз меняя растворы не менее 2 раз, проводят частичную или полную декальцинацию в кислотной среде, обрабатывают перекисью водорода в течение 4 часов, отмывают при комнатной температуре от перекиси водой очищенной, затем отмывают этанолом, высушивают, упаковывают и стерилизуют .
2. Материал для остеопластики и тканевой инженерии, содержащий коллаген, отличающийся тем, что он получен по способу согласно п.l и представляет собой соединение, в котором сохранена нативная, пространственная организация коллагенового матрикса и минеральная составляющая костной ткани природного происхождения, содержащее 20- 25% коллагена и 60-75% минерального вещества.
3. Материал по п. 2, отличающийся тем, что он содержит по данным анализа сухого материала менее 1% неколагенновых белков.
PCT/RU2005/000526 2005-10-27 2005-10-27 Procede de fabrication de materiaux biologiques a partir de tissus osseux et materiau d'osteoplastie et d'ingenierie de tissus obtenu au moyen de ce procede WO2007049988A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US12/091,741 US8241673B2 (en) 2005-10-27 2005-10-27 Method for producing biomaterials from bone tissue and material used for osteoplasty and tissue engineering
EP05857392A EP1941916B1 (en) 2005-10-27 2005-10-27 Method for producing biomaterials from a bone tissue and the thus obtained material used for osteoplasty and tissue engineering
PCT/RU2005/000526 WO2007049988A1 (fr) 2005-10-27 2005-10-27 Procede de fabrication de materiaux biologiques a partir de tissus osseux et materiau d'osteoplastie et d'ingenierie de tissus obtenu au moyen de ce procede
US13/572,793 US8685092B2 (en) 2005-10-27 2012-08-13 Material for osteoplasty and tissue engineering

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/RU2005/000526 WO2007049988A1 (fr) 2005-10-27 2005-10-27 Procede de fabrication de materiaux biologiques a partir de tissus osseux et materiau d'osteoplastie et d'ingenierie de tissus obtenu au moyen de ce procede

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US11/091,741 A-371-Of-International US7892406B2 (en) 2004-03-05 2005-03-28 Ionized physical vapor deposition (iPVD) process
US13/572,793 Continuation-In-Part US8685092B2 (en) 2005-10-27 2012-08-13 Material for osteoplasty and tissue engineering

Publications (1)

Publication Number Publication Date
WO2007049988A1 true WO2007049988A1 (fr) 2007-05-03

Family

ID=37968029

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/RU2005/000526 WO2007049988A1 (fr) 2005-10-27 2005-10-27 Procede de fabrication de materiaux biologiques a partir de tissus osseux et materiau d'osteoplastie et d'ingenierie de tissus obtenu au moyen de ce procede

Country Status (3)

Country Link
US (1) US8241673B2 (ru)
EP (1) EP1941916B1 (ru)
WO (1) WO2007049988A1 (ru)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105854082A (zh) * 2015-01-20 2016-08-17 北京大学第三医院 一种半月板支架及其制备方法
CN107236775A (zh) * 2017-06-05 2017-10-10 深圳知本康业有限公司 一种鹿骨蛋白多肽及应用

Families Citing this family (8)

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EP3417889A1 (en) 2009-12-13 2018-12-26 Amit Prakash Govil Bioactive grafts and composites
DE102010037469A1 (de) * 2010-09-10 2012-03-15 Gelita Ag Verfahren zur Reduzierung des Endotoxingehalts in einem kollagenhaltigen Material
US8980328B2 (en) * 2011-09-29 2015-03-17 Collagen Matrix, Inc. Method of preparing porous carbonate apatite from natural bone
ITVI20120210A1 (it) * 2012-08-10 2014-02-11 Akimedix S R L Un processo per la realizzazione di un rivestimento biocompatibile per impianti ossei con materiale base estratto da osso di mammifero, nonche' rivestimento ed impianto ottenuti
ITVI20120256A1 (it) * 2012-10-04 2014-04-05 Bioteck S P A Un processo per la preparazione di materiali biocompatibili da innesto a partire da osso di mammifero ed innesto biocompatibile ottenuto con il processo
UA115089C2 (uk) 2015-11-04 2017-09-11 Товариство З Обмеженою Відповідальністю "А.А. Партнерс" Медичний продукт на основі клітин людини для корекції остеорепаративних процесів
PL3377226T3 (pl) 2016-03-28 2021-07-26 Illumina, Inc. Wielopłaszczyznowe mikromacierze
CN114209884B (zh) * 2021-12-28 2022-07-05 中国科学院烟台海岸带研究所 一种鱼类脱钙骨基质及其制备方法

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RU2242981C1 (ru) * 2003-10-31 2004-12-27 ЗАО "РеМеТэкс" Биотрансплантат и способ лечения дегенеративных и травматических заболеваний суставного хряща

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WO1998044809A1 (en) * 1997-04-03 1998-10-15 Clearcoll Pty. Ltd. Improved process for the manufacture of collagen
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RU2242981C1 (ru) * 2003-10-31 2004-12-27 ЗАО "РеМеТэкс" Биотрансплантат и способ лечения дегенеративных и травматических заболеваний суставного хряща

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See also references of EP1941916A4 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105854082A (zh) * 2015-01-20 2016-08-17 北京大学第三医院 一种半月板支架及其制备方法
CN105854082B (zh) * 2015-01-20 2019-03-22 北京大学第三医院 一种半月板支架及其制备方法
CN107236775A (zh) * 2017-06-05 2017-10-10 深圳知本康业有限公司 一种鹿骨蛋白多肽及应用

Also Published As

Publication number Publication date
US8241673B2 (en) 2012-08-14
US20080262632A1 (en) 2008-10-23
EP1941916A1 (en) 2008-07-09
EP1941916B1 (en) 2012-08-15
EP1941916A4 (en) 2010-03-24

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