WO2007046398A1 - Promoteur de la nucleostemine specifique d'une cellule souche - Google Patents

Promoteur de la nucleostemine specifique d'une cellule souche Download PDF

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Publication number
WO2007046398A1
WO2007046398A1 PCT/JP2006/320706 JP2006320706W WO2007046398A1 WO 2007046398 A1 WO2007046398 A1 WO 2007046398A1 JP 2006320706 W JP2006320706 W JP 2006320706W WO 2007046398 A1 WO2007046398 A1 WO 2007046398A1
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WO
WIPO (PCT)
Prior art keywords
gene
dna
stem cell
seq
egfp
Prior art date
Application number
PCT/JP2006/320706
Other languages
English (en)
Japanese (ja)
Inventor
Toshio Suda
Atsushi Hirao
Kazuhiro Sakurada
Masahiko Ishihara
Hiromasa Miyaji
Norihiko Shiraishi
Original Assignee
Kyowa Hakko Kogyo Co., Ltd.
Keio University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Hakko Kogyo Co., Ltd., Keio University filed Critical Kyowa Hakko Kogyo Co., Ltd.
Priority to JP2007540995A priority Critical patent/JPWO2007046398A1/ja
Publication of WO2007046398A1 publication Critical patent/WO2007046398A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Veterinary Medicine (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne une technique d'isolement et d'identification d'une cellule souche adulte et un modèle animal permettant d'évaluer aisément la fonction de la cellule souche dans le but d'établir un procédé thérapeutique utilisant la cellule souche adulte. L'invention concerne un ADN présentant une activité de promoteur dans une cellule souche spécifique, un vecteur contenant l'ADN et un mammifère transgénique non humain ou une cellule transformée contenant le vecteur.
PCT/JP2006/320706 2005-10-18 2006-10-18 Promoteur de la nucleostemine specifique d'une cellule souche WO2007046398A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2007540995A JPWO2007046398A1 (ja) 2005-10-18 2006-10-18 幹細胞特異的プロモーター

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2005302630 2005-10-18
JP2005-302630 2005-10-18

Publications (1)

Publication Number Publication Date
WO2007046398A1 true WO2007046398A1 (fr) 2007-04-26

Family

ID=37962491

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2006/320706 WO2007046398A1 (fr) 2005-10-18 2006-10-18 Promoteur de la nucleostemine specifique d'une cellule souche

Country Status (2)

Country Link
JP (1) JPWO2007046398A1 (fr)
WO (1) WO2007046398A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015527083A (ja) * 2012-09-07 2015-09-17 チルドレンズ メディカル センター コーポレーション 造血幹細胞特異的レポーターマウスおよびその使用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001011011A2 (fr) * 1999-08-05 2001-02-15 Mcl Llc Cellules souches adultes toutes-puissantes et procede d'isolement
WO2003102215A2 (fr) * 2002-05-31 2003-12-11 The Board Of Trustees Of The Leland Stanford Junior University Procedes d'identification et d'isolement de cellules souches et de cellules souches cancereuses

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001011011A2 (fr) * 1999-08-05 2001-02-15 Mcl Llc Cellules souches adultes toutes-puissantes et procede d'isolement
WO2003102215A2 (fr) * 2002-05-31 2003-12-11 The Board Of Trustees Of The Leland Stanford Junior University Procedes d'identification et d'isolement de cellules souches et de cellules souches cancereuses

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BADDOO M. ET AL.: "Characterization of Mesenchymal Stem Cells Isolated From Murine Bone Marrow by Negative Selection", J. CELL. BIOCHEM., vol. 89, 2003, pages 1235 - 1249, XP002999369 *
CAI J. ET AL.: "Membrane properties of rat embryonic multipotent neural stem cells", J. NEUROCHEM., vol. 88, 2004, pages 212 - 226, XP002998440 *
DATABASE GENBANK [online] KAUL R.K. ET AL., XP003012049, Database accession no. (AC104446) *
DATABASE GENBANK [online] XP003012050, Database accession no. (AC154727) *
TSAI R.Y.L. ET AL.: "A nucleolar mechanism controlling cell proliferation in stem cells and cancer cells", GENES DEV., vol. 16, 2002, pages 2991 - 3003, XP003012051 *
YE K. ET AL.: "Genetically Engineered Fluorescent Cell Marker for Labeling CD34+ Hematopoietic Stem Cells", BIOTECHNOL. PROG., vol. 20, 2004, pages 561 - 565, XP002393844 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015527083A (ja) * 2012-09-07 2015-09-17 チルドレンズ メディカル センター コーポレーション 造血幹細胞特異的レポーターマウスおよびその使用
US10080354B2 (en) 2012-09-07 2018-09-25 Children's Medical Center Corporation Hematopoietic stem cell specific reporter mouse and uses thereof

Also Published As

Publication number Publication date
JPWO2007046398A1 (ja) 2009-04-23

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