WO2006082264A1 - Procede d'obtention de biostimulants a partir de residus agro-industriels - Google Patents

Procede d'obtention de biostimulants a partir de residus agro-industriels Download PDF

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Publication number
WO2006082264A1
WO2006082264A1 PCT/ES2006/000034 ES2006000034W WO2006082264A1 WO 2006082264 A1 WO2006082264 A1 WO 2006082264A1 ES 2006000034 W ES2006000034 W ES 2006000034W WO 2006082264 A1 WO2006082264 A1 WO 2006082264A1
Authority
WO
WIPO (PCT)
Prior art keywords
agroindustrial
biostimulants
obtaining
phase
procedure
Prior art date
Application number
PCT/ES2006/000034
Other languages
English (en)
Spanish (es)
Inventor
Juan Parrado Rubio
Juan Dionisio Bautista Palomas
Eduardo Jesús ROMERO RAMÍREZ
Original Assignee
Universidad De Sevilla
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad De Sevilla filed Critical Universidad De Sevilla
Publication of WO2006082264A1 publication Critical patent/WO2006082264A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/10—Fertilisers containing plant vitamins or hormones
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
    • C05F5/008—Waste from biochemical processing of material, e.g. fermentation, breweries
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/141—Feedstock
    • Y02P20/145—Feedstock the feedstock being materials of biological origin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Definitions

  • the subject of the present invention is a process for obtaining plant biostimulants from agroindustrial wastes.
  • the process includes: a) A fermentative phase with microorganisms producing hydrolytic enzymes using as a base of the culture medium, agroindustrial residues and CaCI 2 in concentrations that favor the excretion of enzymes and b) a second phase in which the fermentation broths will be used as a hydrolytic tool on new waste that will be modified by the enzymes produced in the first step.
  • Agroindustrial wastes are characterized by being organic matter of high protein content and with a high susceptibility to be hydrolyzed due to the denaturation of the proteins produced in the agroindustrial processes from which they originate.
  • the residues of the bioalcohol industry whose origins are sorghum, corn, wheat and oats seeds, residues of the oil industry (from sunflower seeds, rapeseed, etc.), residues of the wine industry stand out.
  • the final products obtained are natural substances with biostimulant function (stimulates growth, flowering, rooting, ...) and also fertilizer (provides organic nutrients to plants).
  • the current technique makes the low cost (composting) incompatible with the high quality and product control (hydrolysis).
  • the present invention contemplates the use of agro-industrial waste with defined compositions so that the final product has an ideal proportion of nutritional elements for The crop to be treated. It is a process of production of biostimulants through enzymes produced in the same substrates that give rise to biostimulants, reducing process costs.
  • the present invention therefore consists of a two-phase process for obtaining a liquid product rich in soluble phytonutrients and of high absorption, of comparable quality to those obtained by enzymatic hydrolysis but of much lower cost. Description of the invention
  • the present invention aims at a process for obtaining a liquid product rich in soluble phytonutrients and high absorption for use as fertilizers and germination stimulants, rooting, growth, flowering, fruit set and maturation of plants and its fruits It can be applied in its different variants to any type of plant, time of plant development, soil and form of cultivation, and potentially usable in organic farming.
  • the process object of this invention comprises two phases that we will call the fermentation and hydrolytic phase respectively and two types of products are obtained: Soluble substances in liquid form with biostimulant and fertilizer capacity and solid by-product with fertilizer capacity.
  • the raw material used in the process of the present invention as a source of nitrogen and energy for the generation of enzymes are insoluble and defined plant substrates and, generally, of high insolubility. All have as a common characteristic its high content in Protein Nitrogen and a high susceptibility to be hydrolyzed due to the denaturation of the proteins produced in the agro-industrial processes from which they originate.
  • the substrates used in this process will be of vegetable origin and with high protein content as residues of the carob industry, the bioalcohol industry, (with different origins such as corn, sorghum, wheat, or barley), oil industry (sunflower degreased flours , rapeseed, etc.) wine industry (grape pomace) etc.
  • the process of the present invention comprises two phases:
  • the Fermentative Phase it is based on a vegetable substrate with high nitrogen content and high protein denaturation from agro-industrial processes, being the optimal culture medium for Bacillus licheniformis, an enzyme excretory microorganism.
  • a production of a liquid phase enriched in hydrolytic activity of microbial origin must be achieved.
  • a concentration of 0.5% - 2% of plant substrate (the most optimal conditions around 1%) and an inoculum of B. licheniformis together with CaCI 2 in quantities starting from 5 are introduced into a biorector mmolar to favor the synthesis and stability of the enzymes to be produced.
  • the bioreactor must be at neutral pH, T a optimum for the development of the microorganism (35-37 0 C) and aeration for 40-50 hours, obtaining a solution of the modified substrate with a high content of proteases excreted by the microorganism, which is used in Ia Hydrolytic phase of the process as an enzymatic base to increase the productivity of the biostimulant object of the present invention.
  • the Hydrolytic Phase of the process consists in taking the fermentation broth of the fermentation phase and diluting it 20-30 times until reaching an optimum concentration to carry out the hydrolysis. This process requires several controls among which the pH (optimal for enzymatic activity, between 7-8), the temperature (55-6O 0 C) and time (100min-150min) as fundamental factors could be cited.
  • This phase can be carried out in the same container as the previous phase simply by adding water and the agro-industrial waste with high biostimulant potential (10-20% w / v), obtaining the solubility of the introduced plant material with biostimulant capacity.
  • the solid-liquid separation is carried out by centrifugation, at the concentration by evaporation and by acidifying the medium to lower the pH, facilitating storage, transport and, above all, because at that concentration it is totally stable.
  • the resulting solids not solubilized can be used as conventional organic fertilizer and the resulting liquid is the product with biostimulant capacity for use as fertilizers and stimulants of germination, rooting, growth, flowering, fruit set and maturation of your plants and fruits
  • the first phase is the fermentation phase, common in the production of both types of biostimulants, with the difference in the substrate used in the second phase.
  • the fermentation medium includes the following components:
  • microorganisms used are bacteria of the genus Bacillus enzyme superproducers, in the example a strain of Bacillus licheniformis ATCC 21415 has been used.
  • the fermentation phase is centrifuged and the separation is carried out in a liquid and a solid phase.
  • the cell-free liquid phase will be used as a hydrolytic tool because of its high protease content, being cold stable for a month.
  • the second phase is the hydrolysis phase in which the conversion of agroindustrial wastes into biostimulants occurs:
  • the difference lies in the raw material that is hydrolyzed being the process itself in both cases:
  • First Biostimulant called BM From residues of the bioalcohol industry from corn seeds.
  • Second Biostimulant called BA From residues of the food industry of the carob bean.
  • the biostimulant product of corn residues (BM) has the following composition: Physical-chemical characteristics
  • the biostimulant product of carob (BA) has the following composition Physicochemical characteristics
  • biostimulant capacity by shortening flowering times and increase in the number of fruits Using as raw materials corn residues of the bioalcohol industry in the fermentation phase and the carob residues as a substrate in the hydrolytic phase (BA), the biostimulant power It has been studied in tomatoes, in a greenhouse with light and temperature control (Licopericon pimpinellifolium) and a parameter such as the ability to increase the number of total flowers and the total number of fruits has been evaluated.
  • the product has been used in a diluted liquid form for fertirrigation, resulting in its optimal dose ranging from 1/500 dilution of BA.

Abstract

La présente invention concerne un procédé d'obtention de biostimulants végétaux à partir de résidus agro-industriels. Le procédé comprend a) une phase de fermentation avec des microorganismes producteurs d'enzymes hydrolytiques, la base du milieu de culture étant formée de résidus agro-industriels et de CaCI2, selon des concentrations favorisant l'excrétion d'enzymes, et b) une seconde phase dans laquelle les bouillons de fermentation sont réutilisés comme moyen hydrolytique sur de nouveaux résidus modifiés par les enzymes produites au cours de la première phase. Les produits finaux obtenus sont des substances naturelles présentant une fonction biostimulante (stimulant la croissance, la floraison, l'enracinement, ) et fertilisante (fournissant des nutriments organiques aux plantes).
PCT/ES2006/000034 2005-01-28 2006-01-26 Procede d'obtention de biostimulants a partir de residus agro-industriels WO2006082264A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES200500207A ES2259542B1 (es) 2005-01-28 2005-01-28 Procedimiento para la obtencion de bioestimulantes a partir de residuos agroindustriales.
ESP200500207 2005-01-28

Publications (1)

Publication Number Publication Date
WO2006082264A1 true WO2006082264A1 (fr) 2006-08-10

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PCT/ES2006/000034 WO2006082264A1 (fr) 2005-01-28 2006-01-26 Procede d'obtention de biostimulants a partir de residus agro-industriels

Country Status (2)

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ES (1) ES2259542B1 (fr)
WO (1) WO2006082264A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013030422A1 (fr) 2011-08-29 2013-03-07 Heineken España, S.A. Procédé d'obtention de biofertilisants et de biostimulants pour l'agriculture et l'alimentation animale
WO2016005706A1 (fr) 2014-07-11 2016-01-14 Prp Holding Utilisation d'une composition organo-minérale pour augmenter l'assimilation des éléments nutritifs du sol par la plante
CN110999920A (zh) * 2019-12-16 2020-04-14 渭源县农业技术推广中心 一种防治当归病虫害和降低当归抽薹率的复合制剂及其制备方法和应用
WO2020174101A1 (fr) 2019-02-27 2020-09-03 Fertinagro Biotech, S.L. Processus de fermentation biologique à l'état solide

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2571717A1 (fr) * 1984-06-01 1986-04-18 Guillon Genevieve Procede de compostage accelere de dechets contenant des matieres cellulosiques
KR880000583B1 (ko) * 1985-05-03 1988-04-16 도만영 폐수처리용 활성오니 영양제 조성물의 제조방법
JPH08157285A (ja) * 1994-12-01 1996-06-18 Shikoku Keisoku Kogyo Kk 植物性廃棄物の液状堆肥化方法
JPH10328643A (ja) * 1997-05-28 1998-12-15 Kooyo Techno Kk 有機物含有廃棄物の醗酵分解方法および醗酵分解装置
JPH11217286A (ja) * 1998-02-03 1999-08-10 Nisshin Engineering:Kk 有機質の発酵堆肥の製造方法及び製造装置
JP2000107794A (ja) * 1998-10-07 2000-04-18 Sanei Kogyo Kk 有機性廃棄物の処理方法とこれに用いる処理装置
JP2001354488A (ja) * 2000-06-07 2001-12-25 Chumoku Co Ltd 木質系廃材の急速コンポスト化方法
US20040033290A1 (en) * 2001-01-10 2004-02-19 Yutaka Matano Vegetable fiber-digesting agent and method of processing vegetable waste by using the same
CN1482105A (zh) * 2003-07-24 2004-03-17 武汉工业学院 利用皂素废渣和废水发酵制备有机肥料的方法

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2571717A1 (fr) * 1984-06-01 1986-04-18 Guillon Genevieve Procede de compostage accelere de dechets contenant des matieres cellulosiques
KR880000583B1 (ko) * 1985-05-03 1988-04-16 도만영 폐수처리용 활성오니 영양제 조성물의 제조방법
JPH08157285A (ja) * 1994-12-01 1996-06-18 Shikoku Keisoku Kogyo Kk 植物性廃棄物の液状堆肥化方法
JPH10328643A (ja) * 1997-05-28 1998-12-15 Kooyo Techno Kk 有機物含有廃棄物の醗酵分解方法および醗酵分解装置
JPH11217286A (ja) * 1998-02-03 1999-08-10 Nisshin Engineering:Kk 有機質の発酵堆肥の製造方法及び製造装置
JP2000107794A (ja) * 1998-10-07 2000-04-18 Sanei Kogyo Kk 有機性廃棄物の処理方法とこれに用いる処理装置
JP2001354488A (ja) * 2000-06-07 2001-12-25 Chumoku Co Ltd 木質系廃材の急速コンポスト化方法
US20040033290A1 (en) * 2001-01-10 2004-02-19 Yutaka Matano Vegetable fiber-digesting agent and method of processing vegetable waste by using the same
CN1482105A (zh) * 2003-07-24 2004-03-17 武汉工业学院 利用皂素废渣和废水发酵制备有机肥料的方法

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013030422A1 (fr) 2011-08-29 2013-03-07 Heineken España, S.A. Procédé d'obtention de biofertilisants et de biostimulants pour l'agriculture et l'alimentation animale
WO2016005706A1 (fr) 2014-07-11 2016-01-14 Prp Holding Utilisation d'une composition organo-minérale pour augmenter l'assimilation des éléments nutritifs du sol par la plante
WO2020174101A1 (fr) 2019-02-27 2020-09-03 Fertinagro Biotech, S.L. Processus de fermentation biologique à l'état solide
CN110999920A (zh) * 2019-12-16 2020-04-14 渭源县农业技术推广中心 一种防治当归病虫害和降低当归抽薹率的复合制剂及其制备方法和应用

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ES2259542B1 (es) 2007-10-01
ES2259542A1 (es) 2006-10-01

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