WO2006028332A1 - Prmoteur inductible par le sucrose tire de la patate douce - Google Patents

Prmoteur inductible par le sucrose tire de la patate douce Download PDF

Info

Publication number
WO2006028332A1
WO2006028332A1 PCT/KR2005/002820 KR2005002820W WO2006028332A1 WO 2006028332 A1 WO2006028332 A1 WO 2006028332A1 KR 2005002820 W KR2005002820 W KR 2005002820W WO 2006028332 A1 WO2006028332 A1 WO 2006028332A1
Authority
WO
WIPO (PCT)
Prior art keywords
sucrose
plant
gene
inducible
promoter
Prior art date
Application number
PCT/KR2005/002820
Other languages
English (en)
Inventor
Jung Myung Bae
Man Sup Kwak
Seol Ah Noh
Jeong Sheop Shin
Shin Woo Lee
Original Assignee
Korea University Industry and Academy Cooperation Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea University Industry and Academy Cooperation Foundation filed Critical Korea University Industry and Academy Cooperation Foundation
Priority to JP2006553068A priority Critical patent/JP2007521830A/ja
Priority to CN200580003363XA priority patent/CN1914321B/zh
Priority to US10/574,842 priority patent/US7538210B2/en
Publication of WO2006028332A1 publication Critical patent/WO2006028332A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Definitions

  • the present invention relates to a plant sucrose-inducible promoter sequence. More specifically, the present invention relates to a plant sucrose-inducible promoter sequence which originates from sweetpotato ADP-glucose pyrophosphorylase gene ( ibAGPl) and can confer a high level of sucrose-inducible expression in plants. In addition, the present invention relates to plant sucrose-inducible expression vectors comprising the promoter sequence, and to production of a transgenic plant using the promoter sequence.
  • ibAGPl sweetpotato ADP-glucose pyrophosphorylase gene
  • sucrose-inducible promoters whose activities increase upon treatment with sucrose have been actively studied.
  • Such sucrose-inducible promoters have merits when applied to the mass production of useful medicinal and industrial proteins in plant storage organ tissues, such as storage roots, which contain starch synthesized from sucrose in relatively large quantities.
  • useful proteins include very valuable and expensive medicinal or industrial proteins, such as interferon, growth hormones, Lactoferrin, and phytase.
  • sucrose-inducible promoters have been focused on genes coding storage proteins accumulated in storage organ tissues or genes relating to the synthesis of starch.
  • patatin is a storage protein in the potato. It has been identified that the activity of a patatin gene promoter is increased by sucrose (Rocha-Sosa et al., 1989, EMBO J 8, 23-31; Wenzler et al., 1989a, Plant MoI. Biol. 12, 41-50; Wenzler et al., 1989b, Plant MoI. Biol. 13, 347-354). It has also been reported that a specific nucleotide sequence of the -344 region of the promoter (B sequence) plays an essential role in sucrose induction (Grierson et al., 1994, Plant J, 5, 815-826).
  • sucrose synthase genes there are two sucrose synthase genes, Sus3 and Sus4, in the potato. It has been identified that Sus4, among the two genes, is expressed by sucrose (Salanoubat and Belliard, 1989, GENE 84, 181-185). In addition, it has been reported that the -1500 ⁇ -267 region of the Sus4 gene promoter, the 3' untranslated region, and a 1612 bp leader intron are essential for sucrose induction (Fu et al., 1995, Plant Cell 7, 1387-1394).
  • ADP-glucose pyrophosphorlyase is believed to be the key regulatory enzyme in controlling the amount of starch in plants.
  • the ADP-glucose pyrophos ⁇ phorlyase gene promoters in tomato and Arabidopsis have been reported to be sucrose- inducible (Siedlecka et al., 2003, Planta 217, 184-192; Li et al., 2002, Plant Science 162, 239-244).
  • the activity of the promoter in Arabidopsis is decreased by okadaic acid, which is an inhibitor of protein phosphatase I and 2A.
  • an object of the present invention is to provide a sucrose-inducible promoter sequence that can induce a high level expression of target genes and that is derived from the sweetpotato ADP-glucose py- rophosphorlyase gene (JbAGPl).
  • Another object of the present invention is to provide a sucrose-inducible vector for plant transformation comprising the sucrose-inducible promoter sequence directing a high level of expression of target genes and a 5' untranslated region of the ibAGPl gene.
  • a still further object of the present invention is to provide a transgenic plant transformed with the same vector, capable of producing useful materials in large quantities in storage organ tissues of plants, such as in storage roots.
  • the present inventors have cloned a sucrose-inducible promoter region of the sweetpotato ADP-glucose pyrophosphorlyase gene (ibAGPl) and constructed a vector for plant transformation and a transient expression vector comprising the above promoter with the 5' untranslated region of the same gene. These inventors have subsequently induced expression in the storage roots using the same vectors, and observed a high level of activity of the promoter with regard to sucrose inducibility to accomplish the present invention.
  • ibAGPl sweetpotato ADP-glucose pyrophosphorlyase gene
  • the present invention provides the isolated DNA sequence of the sucrose-inducible promoter region and the 5' untranslated region of the sweetpotato ADP-glucose pyrophosphorlyase gene (ibAGPl) comprising the sequences of SEQ ID NO: 1.
  • the high levels of activity of the promoter according to the present invention can be induced by sucrose in plants.
  • the above untranslated region comprises the untranslated region of bp +1 to +68 relative to the transcription initiation site of the sweetpotato ADP-glucose pyrophos- phorlyase gene (ibAGPl) in SEQ ID NO: l(see Fig. 1).
  • the untranslated region according to the present invention possibly can induce a high level of expression of the target gene, like the other reported 5' untranslated regions of plants, by enhancing the translation efficacy of a target foreign gene introduced into the plant.
  • the present invention provides a sucrose- inducible vector for plant transformation (pSPagpl-101) and a transient expression vector (pSPagp 1-221), comprising a plant sucrose-inducible promoter directing high levels of expression in plants and a 5' untranslated region of the sweetpotato ADP- glucose pyrophosphorlyase gene (ibAGPl).
  • sucrose-inducible vector for plant transformation means a binary vector that can permanently express foreign genes in transgenic plants.
  • the above transient expression vector means a vector that can transiently express foreign genes in plants.
  • the binary vector can be any binary vector comprising the RB and LB of T-DNA that can transform the plant in the presence of the Ti plasmid of Agrobacterium tumefaciens.
  • it may be a binary vector frequently used in the related field, such as the pBIlOl (cat#: 6018-1, Clonetech, USA), pBIN (Genbank accession NO. U09365), pBI121, pBIN20, or BIBAC vectors.
  • sucrose-inducible vectors pSPagp 1-101, pSPagp 1 -221
  • the promoter and 5' untranslated region of ADP- glucose pyrophosphorlyase gene ibAGPl
  • the present invention provides pSPagpl-101 and pSPagpl-221 (see Figs.
  • the GUS reporter gene is a foreign gene, and is expected to be replaceable with any other useful foreign gene.
  • the present invention provides a transgenic plant using the sucrose-inducible binary vector according to the present invention. Further the present invention provides a storage root transiently transformed using the transient expression vector according to the present invention.
  • Plants can be transformed with the above plant sucrose -inducible binary vector using, for example, Agrobacterium tumefaciens (An, G. 1987, Plant Physiology) or the method of particle bombardment (Lacorte et al., 1997, Plant Cell Reports).
  • Agrobacterium tumefaciens As, G. 1987, Plant Physiology
  • particle bombardment Lacorte et al., 1997, Plant Cell Reports.
  • Arabidopsis is transformed using the floral dip method (Clough and Bent, 1998, Plant J.).
  • Plant storage roots can be transiently transformed using the transient expression vector, according to the present invention, and the particle bombardment method.
  • the transient expression vector of the present invention can transform storage roots regardless of the kind of crop. Examples of crops include carrots, etc.
  • the above foreign gene may be any gene that is intended to be expressed in large quantities in plant storage organ tissues which contain sucrose in relatively large quantities to accumulate starch in large quantities in the plant. Furthermore, the foreign genes are located next to the promoter and 5' untranslated region of the sweetpotato ADP-glucose pyrophosphorlyase gene (JbAGPl) in the plant sucrose-inducible vector according to the present invention and may be expressed in the form of being fused with the reporter genes if necessary.
  • JbAGPl sweetpotato ADP-glucose pyrophosphorlyase gene
  • the present invention provides PCR primers of SEQ ID NO: 2 ⁇ SEQ ID NO: 5 suitable for amplifying DNA fragment of plant sucrose-inducible promoter according to the present invention.
  • the present invention provides a sucrose-inducible promoter and 5' untranslated region of the ADP-glucose pyrophosphorlyase gene (JbAGPl) from sweetpotato ( Ipomoea batatas).
  • the promoter and 5' untranslated region according to the present invention can confer sucrose-inducible expression in plants, and, particularly, can confer high levels of expression in plant storage roots which contain sucrose in relatively large quantities to accumulate starch in large quantities in the plants.
  • the present invention may be useful for the generation of transgenic plants to produce useful proteins in large quantities in plant storage roots.
  • useful proteins include very valuable and expensive medicinal or industrial proteins, such as interferon, growth hormones, Lactoferrin, and phytase.
  • FIG. 1 shows DNA sequences of a plant sucrose-inducible promoter and a 5' un ⁇ translated region of the sweetpotato ADP-glucose pyrophosphorlyase gene (JbAGPl) according to the present invention
  • Fig. 2 shows a diagrammatic representation of a binary vector for plant trans ⁇ formation (hereinafter referred to as 'pSPagpl-101') comprising sequences of plant sucrose-inducible promoter and a 5' untranslated region of the sweetpotato ADP- glucose pyrophosphorlyase gene (JbAGPl) according to the present invention;
  • FIG. 3 shows a diagrammatic representation of a transient expression vector
  • 'pSPagpl-221' comprising sequences of a plant sucrose- inducible promoter and a 5' untranslated region of the sweetpotato ADP-glucose py- rophosphorlyase gene (JbAGPl) according to the present invention
  • FIG. 4 shows GUS expression patterns of each tissue observed after histochemical staining of Arabidopsis transformed with the pSPagpl-101;
  • Fig. 5 shows the results of quantitative analysis of GUS after sucrose treating of
  • FIG. 6 shows results of transient assay with carrot taproots using pSPagp 1-221 according to the present invention. Best Mode for Carrying Out the Invention
  • Example 1 cloning of the sucrose-inducible promoter of the sweetpotato ADP- glucose pyrophosphorlyase gene ( ibAGPl )
  • a promoter of the sweetpotato ADP-glucose pyrophosphorlyase gene was identified in the 5' region sequence of the sweetpotato ADP-glucose pyrophos ⁇ phorlyase gene (ibAGPl, Noh et al., GENE, 2004, 339, 173-180).
  • Fig. 1 shows DNA sequences of a plant sucrose- inducible promoter and a 5' untranslated region of the sweetpotato ADP-glucose py ⁇ rophosphorlyase gene (ibAGPl) according to the present invention.
  • ibAGPl the sweetpotato ADP-glucose py ⁇ rophosphorlyase gene
  • Example 2 construction of plant sucrose-inducible vector and a transient expression vector
  • the sweetpotato ADP-glucose pyrophosphorlyase gene (ibAGPl) promoter cloned in example 1 and a 68 bp 5' untranslated region (SEQ NO ID: 1, Fig. 1) were inserted into pBHOl or pBI221 (Clonetech) to construct a plant sucrose-inducible vector or a transient expression vector respectively.
  • the sweetpotato ADP-glucose pyrophosphorlyase gene (ibAGPl) promoter, cloned in example 1, and a 68 bp 5' untranslated region (SEQ NO ID : 1, see Fig. 1) were amplified by PCR and digested with Sail and BamHI. Then they were inserted into the Sail and BamHI sites of pBHOl.
  • the vector was termed as pSPagpl-101 (see Fig. 2).
  • the primers used in the above PCR are shown in Table 1 in detail.
  • GUS is a reporter gene which encodes ⁇ -glucuronidase and selectable marker is kanamycin.
  • Nos-pro represents a promoter of NPTII and Nos-ter represents a terminator thereof.
  • the GUS reporter gene is expressed by an inserted promoter and terminator (Nos-ter) of Nos (Nopalin synthase) in plants.
  • the sweetpotato ADP-glucose pyrophosphorlyase gene (ibAGPl) promoter, cloned in example 1, and a 68 bp 5' untranslated region (SEQ NO ID : 1, see Fig. 1) were amplified by PCR and digested with Sphl and BamHI. Then they were inserted into the Sphl and BamHI sites of pBI221.
  • the vector was termed as pSPagp 1-221 (see Fig. 3).
  • the primers used in the above PCR are shown in Table 2 in detail.
  • Example 3 transformation of Arabidopsis using the pSPagpl-101 vector constructed in Example 2
  • the pSPagpl-101 vector constructed in Example 2 was transferred to Agrobacterium tumefaciens C58C1 using the Freeze-thaw method (An, G. 1987, Methods in Enzymology).
  • Example 4 histochemical staining and enzvmological assay of transformed Arabidopsis
  • Example 5 identification of the activity of the sucrose-inducible promoter ( ibAGPl promoter * ) according to the present invention
  • sucrose-inducible activity of the ibAGPl promoter was examined quantitatively with sucrose-treated transformed Arabidopsis using the method of Jefferson et al., (EMBO J. 6: 3901-3907, 1987).
  • the GUS activity shown in Fig. 5 was obtained by analyzing the twelve transformants (T2 line plant) treated with each sucrose concentration. For comparison, the pBlOl vector without the promoter and pB121 (Clonetech, USA) with the CaMV35S promoter were examined together. The results revealed that the GUS activity of the ibAGPl gene promoter increased 8 times upon treatment with 3% sucrose, and increased 11 times upon treatment with 6% sucrose. In addition, the GUS activity of the ibAGPl gene promoter increased 12 - 15 times compared to the universal promoter, CaMV35S.
  • Example 6 identification of the activity of the sucrose-inducible promoter ( ibAGPl promoter) in carrot taproots
  • the transient assay method was carried out using pSPagp 1-221 constructed in Example 2.
  • the taproots of carrot in growth and enlargement stages were picked and washed. Then the taproots were transversely cut to 5 mm thick and placed on fully wet 3MM paper in Petri dishes for 4 - 5 hours at 4°C.
  • the method of Sanford et al. (1993, Meth Enzymol 217:485-509) According to the method of Sanford et al. (1993, Meth Enzymol 217:485-509),
  • DNA was mixed and coated onto gold particles 1.0 D in diameter.
  • bombarding conditions were used; [1.0 D DNA/bombardment, 1,350 PSi pressure of helium gas, and a distance of 6 cm from carrots].
  • the present invention provides sucrose-inducible promoter
  • the present invention provides the plant binary vector and the transient expression vector prepared by inserting the promoter and the 5' untranslated region into pBHOl or pBI221, respectively.
  • JbAGPl the promoter and 5' untranslated region of the sweetpotato ADP-glucose pyrophos- phorlyase gene according to the present invention can confer sucrose- inducible expression, and particularly can confer high levels of expression in plant storage roots which contain sucrose in relatively large quantities to accumulate starch in large quantities in plants. Therefore, the present invention may be useful for the generation of transgenic plants to produce useful proteins in large quantities in plant storage roots. Also, the present invention may be useful for studies in areas such as the metabolic engineering of storage roots using transformants or the production of functional materials.

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Cette invention concerne une nouvelle séquence de promoteur inductible par le sucrose et une région non traduite en 5' qui sont dérivées du gène ADP-glucose pyrophosphorlyase de la patate douce (ibAGPl) (numéro d'identification de séquence: 1). Cette invention concerne également des vecteurs d'expression utilisant ces séquences et une plante transgénique utilisant ces vecteurs. Ce promoteur et cette région non traduite en 5' peuvent conférer aux plantes un niveau élevé d'expression inductible par le sucrose, en particulier dans les racines tubéreuses des plantes, qui contiennent du sucrose en quantités relativement importantes, de façon à accumuler de l'amidon en grandes quantités dans la plante. Cette invention peut par conséquent être utile pour produire des plantes transgéniques destinées à fabriquer des protéines utiles en grandes quantités dans les racines tubéreuses des plantes.
PCT/KR2005/002820 2004-09-06 2005-08-25 Prmoteur inductible par le sucrose tire de la patate douce WO2006028332A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2006553068A JP2007521830A (ja) 2004-09-06 2005-08-25 サツマイモ由来糖誘導性プロモータ
CN200580003363XA CN1914321B (zh) 2004-09-06 2005-08-25 来自甘薯的蔗糖诱导型启动子
US10/574,842 US7538210B2 (en) 2004-09-06 2005-08-25 Sucrose-inducible promoter from sweetpotato

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020040070820A KR100604191B1 (ko) 2004-09-06 2004-09-06 고구마 유래 식물체 당 유도성 프로모터 염기서열 및 이를포함하는 식물체 당 유도성 발현 벡터
KR10-2004-0070820 2004-09-06

Publications (1)

Publication Number Publication Date
WO2006028332A1 true WO2006028332A1 (fr) 2006-03-16

Family

ID=36036593

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2005/002820 WO2006028332A1 (fr) 2004-09-06 2005-08-25 Prmoteur inductible par le sucrose tire de la patate douce

Country Status (5)

Country Link
US (1) US7538210B2 (fr)
JP (1) JP2007521830A (fr)
KR (1) KR100604191B1 (fr)
CN (1) CN1914321B (fr)
WO (1) WO2006028332A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101510828B1 (ko) 2013-10-17 2015-04-09 고려대학교 산학협력단 식물체 전신 고 발현 재조합 프로모터
KR101965971B1 (ko) 2017-09-28 2019-04-04 경희대학교 산학협력단 인산 결핍 유도 프로모터 및 이의 용도
KR101963971B1 (ko) 2017-09-28 2019-03-29 경희대학교 산학협력단 가뭄 스트레스 유도성 프로모터 및 이의 용도
KR101922429B1 (ko) 2017-10-13 2019-02-20 대한민국 국화 유래 전신 발현 프로모터 및 이의 용도
KR102000471B1 (ko) 2018-06-19 2019-07-16 대한민국 국화 유래 전신 발현 프로모터 및 이의 용도
KR102065490B1 (ko) 2019-08-30 2020-01-13 경희대학교 산학협력단 벼 Os07g39940 유전자 유래의 뿌리털 특이적 프로모터 및 이의 용도
KR102065488B1 (ko) 2019-08-30 2020-01-13 경희대학교 산학협력단 벼 Os12g39850 유전자 유래의 뿌리털 특이적 프로모터 및 이의 용도
KR102065491B1 (ko) 2019-08-30 2020-01-13 경희대학교 산학협력단 벼 Os11g41640 유전자 유래의 뿌리털 특이적 프로모터 및 이의 용도
KR102065493B1 (ko) 2019-08-30 2020-01-13 경희대학교 산학협력단 벼 Os03g42100 유전자 유래의 뿌리털 특이적 프로모터 및 이의 용도
KR102390866B1 (ko) 2020-05-18 2022-04-26 대한민국 국화 유래 전신 발현 프로모터 및 이의 용도
CN114717236B (zh) * 2022-04-24 2023-10-10 西南大学 一种辣椒受外源糖添加诱导型启动子的克隆及应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2213991C (fr) * 1995-12-27 2005-09-13 Japan Tobacco Inc. Sequence promotrices inductibles a froid
JP3571639B2 (ja) * 1999-10-29 2004-09-29 コリア リサーチ インスティチュート オブ バイオサイエンス アンド バイオテクノロジー さつまいも由来の新規ぺルオキシダーゼ遺伝子及びそのプロモーター
US7041815B2 (en) 2001-05-08 2006-05-09 Sinon Corporation Sporamin promoter and uses thereof
KR100542053B1 (ko) * 2004-01-17 2006-01-12 학교법인고려중앙학원 고구마 에이디피-글루코즈 인산화 효소 유전자에서 유래된엽록체 타겟팅용 디엔에이 염기서열 및 이를 포함하는플라스미드 벡터

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BAE J.M. & LIU J.R.: "Molecular cloning and characterization of two novel isoforms of the small subunit of ADP glucose pyrophosphorylase form sweet potato", MOL GEN GENET, vol. 254, no. 2, 1997, pages 179 - 185 *
HARN C.H. ET AL: "Presence of multiple cDNAs encoding an isoform of ADP-glucose pyrophosphorylase large subunit from sweet potato and characterization of expression levels.", PLANT CELL PHYSIOL, vol. 41, no. 11, 2000, pages 1235 - 1242 *
LI X. ET AL: "Sucrose regulation of ADP-glucose pyrophosphorylase subunit genes transcript levels in leaves and fruits", PLANTA, vol. 162, no. 2, 2002, pages 239 - 244 *
SIEDLECKA A. ET AL: "The small subunit ADP-glucose pyrophosphorylase ( ApS) promoter mediates okadaicacid-sensitive uidA expression in starch-synthesizing tissues and cells in Arabidopsis.", PLANTA, vol. 217, no. 2, 2003, pages 184 - 192 *

Also Published As

Publication number Publication date
CN1914321A (zh) 2007-02-14
CN1914321B (zh) 2011-07-20
US20060288452A1 (en) 2006-12-21
KR20060022054A (ko) 2006-03-09
US7538210B2 (en) 2009-05-26
JP2007521830A (ja) 2007-08-09
KR100604191B1 (ko) 2006-07-25

Similar Documents

Publication Publication Date Title
US7538210B2 (en) Sucrose-inducible promoter from sweetpotato
US7273967B2 (en) Sweet potato MADS-box promoter directing high level expression in plant storage root
US20210102218A1 (en) Expression of transcription regulators that provide heat tolerance
Yamchi et al. Proline accumulation in transgenic tobacco as a result of expression of Arabidopsis Δ 1-pyrroline-5-carboxylate synthetase (P5CS) during osmotic stress
CN105198976B (zh) 一种与植物抗逆性相关蛋白GsERF6及其编码基因与应用
KR101640586B1 (ko) 키위 유래 화분 또는 화분관 특이적 프로모터 및 이의 용도
US20230058847A1 (en) Enhancer
CN104829699A (zh) 一种与植物抗逆性相关蛋白Gshdz4及其编码基因与应用
US20220325293A1 (en) Promoters for regulation of gene expression in plants
Hong et al. Promoter of chrysanthemum actin confers high-level constitutive gene expression in Arabidopsis and chrysanthemum
US9458455B2 (en) High efficiency plant expression promoter from Capsicum annuum serine hydroxymethyl transferase gene and uses thereof
CN112538107B (zh) 三萜相关蛋白质与其编码基因以及在提高植物三萜化合物含量中的应用
CN110294795B (zh) 大豆蛋白质GmDISS2及其编码基因在调控植物耐逆性中的应用
KR100859988B1 (ko) 지상부 특이적 프로모터 및 이를 이용한 목적 단백질의지상부 특이적 발현 방법
Ahmadi et al. The promoter of a metallothionein-like gene from the tropical tree Casuarina glauca is active in both annual dicotyledonous and monocotyledonous plants
JP2004528854A (ja) 新規構成的植物プロモーター
CN113773375B (zh) 大豆核因子蛋白GmNF307在植物耐盐调控中的应用
CN112552383B (zh) 转录因子hinge1在调控植物氮-磷稳态中的应用
WO2009022845A2 (fr) Promoteur d'un fort niveau d'expression dans une culture de tissus végétaux, et vecteur l'utilisant
KR101825960B1 (ko) 벼 유래 뿌리 특이적 프로모터 및 이의 용도
CN116732000A (zh) 一种构树低温胁迫相关的蛋白及其编码基因与应用
CN112048490A (zh) 棉花丝/苏氨酸蛋白磷酸酶GhTOPP6及其编码基因和应用
CN116836249A (zh) 与小麦产量相关的三个同源基因及相关蛋白质
EP2423316B1 (fr) Procédé pour déterminer la fréquence de la recombinaison meiotique dans les plantes

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KM KP KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NG NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SM SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU LV MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 2006288452

Country of ref document: US

Ref document number: 10574842

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2006553068

Country of ref document: JP

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 200580003363.X

Country of ref document: CN

WWP Wipo information: published in national office

Ref document number: 10574842

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase