WO2005107938A2 - Dispositif de réaction thermique et méthode d'utilisation de semblable - Google Patents

Dispositif de réaction thermique et méthode d'utilisation de semblable Download PDF

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Publication number
WO2005107938A2
WO2005107938A2 PCT/US2005/015352 US2005015352W WO2005107938A2 WO 2005107938 A2 WO2005107938 A2 WO 2005107938A2 US 2005015352 W US2005015352 W US 2005015352W WO 2005107938 A2 WO2005107938 A2 WO 2005107938A2
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WO
WIPO (PCT)
Prior art keywords
thermal
reaction
channel
microfluidic
ofthe
Prior art date
Application number
PCT/US2005/015352
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English (en)
Other versions
WO2005107938A3 (fr
Inventor
Federico Goodsaid
Marc A. Unger
Jiang Huang
Emerson Quan
Robert Grossman
Phillip Lam
Hou-Pu Chou
Jake Kimball
Martin Pieprzyk
Geoffrey Facer
Original Assignee
Fluidigm Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/837,885 external-priority patent/US7476363B2/en
Priority claimed from US10/876,046 external-priority patent/US20050145496A1/en
Priority claimed from US11/043,895 external-priority patent/US8105553B2/en
Priority claimed from US11/058,106 external-priority patent/US7867763B2/en
Application filed by Fluidigm Corporation filed Critical Fluidigm Corporation
Priority to EP05766055.7A priority Critical patent/EP1796824A4/fr
Priority to CN2005800225837A priority patent/CN1997454B/zh
Priority to JP2007511507A priority patent/JP5502275B2/ja
Publication of WO2005107938A2 publication Critical patent/WO2005107938A2/fr
Publication of WO2005107938A3 publication Critical patent/WO2005107938A3/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/527—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for microfluidic devices, e.g. used for lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00353—Pumps
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00389—Feeding through valves
    • B01J2219/004—Pinch valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00389—Feeding through valves
    • B01J2219/004—Pinch valves
    • B01J2219/00403—Pinch valves in multiple arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00495—Means for heating or cooling the reaction vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00572—Chemical means
    • B01J2219/00576—Chemical means fluorophore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B01J2219/00587—High throughput processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0638—Valves, specific forms thereof with moving parts membrane valves, flap valves

Definitions

  • FIG. 3B is a schematic representation of a more complex blind channel device based upon the unit of the general design depicted in FIG. 3 A.
  • FIGS. 15 is a schematic diagram of the microfluidic device used for the experiments in Example 4.
  • Fig. 29C is a simplifed plan view of a portion of the carrier of Fig. 28B;
  • Elastomeric materials having a Young's modulus outside of these ranges can also be utilized depending upon the needs of a particular application.
  • the array format of certain of the devices means the devices can achieve high throughput.
  • the high throughput and temperature control capabilities make the devices useful for performing large numbers of nucleic acid amplifications (e.g., polymerase chain reaction - PCR).
  • nucleic acid amplifications e.g., polymerase chain reaction - PCR
  • Such reactions will be discussed at length herein as illustrative of the utility of the devices, especially of their use in any reaction requiring temperature control.
  • the devices can be utilized in a wide variety of other types of analyses or reactions. Examples include analyses of protein-ligand interactions and interactions between cells and various compounds. Further examples are provided infra.
  • the method include having a step of removing the pattern of reacted photoresist material; having the removing is caused by applying an adhesive tape to the surface of the elastomeric layer and the pattern of reacted photoresist material, then separating the adhesive tape from the elastomeric layer while some or all of the pattern of reacted photoresist material is removed from the surface of the elastomeric layer; having the photo resist be SU8; having the etching reagent comprises tetrabutylammoniumfluoride-trihydrate; having the feature be a via; having the elastomeric block comprise a plurality of elastomeric layers bonded together, wherein two or more elastomeric layers have recesses formed therein and one recess of one elastomeric layer is in communication with a recess of another elastomeric layer through the via.
  • FIG.3A is shown in FIG. 3B.
  • the device shown in FIG.3B is one in which the unit organization of the horizontal and branch flow channels 302 shown in FIG. 3 A is repeated multiple times.
  • the device shown in FIG. 3B further illustrates the inclusion of guard channels 320 in those devices to be utilized in applications that involve heating (e.g., thermocycling).
  • An exemplary orientation of the guard channels 320 with respect to the flow channels 304 and branch channels 306 is shown in the enlarged view depicted in the right panel of Fig. 3B.
  • the guard channels 320 overlay the branch flow channels 306 and reaction sites 308. As discussed above, water is flowed through the guard channels 320 during heating of the device 300 to increase the local concentration of water in the device, thereby reducing evaporation of water from solution in the flow channels 306 and reaction sites 308.
  • some devices have densities of at least 100, 150, 200, 250, 300, 400, 500, 600, 700, 800, 900 or 1000 sites/ cm 2 .
  • Devices with very high densities of at least, 2000, 3000, or 4000 sites/ cm 2 are also obtainable. Such high densities directly translate into a very large number of reaction sites on the device.
  • Devices utilizing the blind channel architecture typically have at least 10-100 reaction sites, or any integral number of sites therebetween. More typically, the devices have at least 100- 1,000 reaction sites, or any integral number of sites therebetween. Higher density devices can have even more reaction sites, such as at least 1,000- 100,000 reaction sites, or any integral number of sites therebetween.
  • Device 400 comprises a plurality of horizontal flow channels 404, each of which has a plurality of branch flow channels 406 extending from it and its own sample inlet 414.
  • a control channel 410 overlays each of the branch flow channels 406 and membrane (valve) 412 separates the control channel 410 from the underlying branch flow channel 406.
  • membrane (valve) 412 separates the control channel 410 from the underlying branch flow channel 406.
  • actuation of the control channel at inlet 416 causes deflection of membranes 412 into the branch flow channels 406 and isolation of reaction sites 408.
  • each horizontal flow channel 404 can include an inlet 414 at each end, thus allowing sample to be introduced from both ends.
  • pyroelectric sensors Specifically, some crystalline materials, particularly those materials also exhibiting piezoelectric behavior, exhibit the pyroelectric effect. This effect describes the phenomena by which the polarization of the material's crystal lattice, and hence the voltage across the material, is highly dependent upon temperature. Such materials could be incorporated onto the substrate or elastomer and utilized to detect temperature.
  • FIG. 5 illustrates the rapidity at which the desired temperature is achieved using a blind channel device.
  • Detectors can be microfabricated within the microfluidic device, or can be a separate element. If the detector exists as a separate element and the microfluidic device includes a plurality of detection sections, detection can occur within a single detection section at any given moment.
  • scanning systems can be used. For instance, certain automated systems scan the light source relative to the microfluidic device; other systems scan the emitted light over a detector, or include a multichannel detector.
  • the microfluidic device can be attached to a translatable stage and scanned under a microscope obj ective. A signal so acquired is then routed to a processor for signal interpretation and processing. Arrays of photomultiplier tubes can also be utilized. Additionally, optical systems that have the capability of collecting signals from all the different detection sections simultaneously while determining the signal from each section can be utilized.
  • the detector can also include, for example, a temperature sensor, a conductivity sensor, a potentiometric sensor (e.g., pH electrode) and/or an amperometric sensor (e.g., to monitor oxidation and reduction reactions).
  • a temperature sensor e.g., a thermocouple
  • a conductivity sensor e.g., a thermocouple
  • a potentiometric sensor e.g., pH electrode
  • an amperometric sensor e.g., to monitor oxidation and reduction reactions.
  • a Scorpion (as shown in Fig.24) consists of a specific probe sequence that is held in a hairpin loop configuration by complementary stem sequences on the 5' and 3' sides of the probe. The fluorophore attached to the 5'-endis quenched by a moiety (normally methyl red) joined to the 3'-end of the loop. The hairpin loop is linked to the 5'-end of a primer via a PCR stopping sequence (stopper). After extension of the primer during PCR amplification, the specific probe sequence is able to bind to its complement within the same strand of DNA.
  • amplification reactions can be linear amplifications, (amplifications with a single primer), as well as exponential amplifications (i.e., amplifications conducted with a forward and reverse primer set).
  • NASBA nucleic acid based sequence amplification
  • nucleic acid sample comprising mRNA franscript(s) of the gene(s) or gene fragments, or nucleic acids derived from the mRNA transcript(s) is obtained.
  • a nucleic acid derived from an mRNA transcript refers to a nucleic acid for whose synthesis the mRNA transcript or a subsequence thereof has ultimately served as a template.
  • Anays of materials may also be formed by the methods described in
  • Fig. 25 depicts a cut-away side-view of platen 3207 urged against upper face 3221 of microfluidic device 3205.
  • FIG. 27A and 27B depict a particular embodiment of system 3500, and more specifically, of interface plate 3520.
  • interface plate 3520 is coupled to the integrated chip and carrier 3400 in a manner that fluidly seals certain regions thereof.
  • fluid seals are provided between interface plate 3520 and one or more regions of carrier 3400 and chip, such as the first protein region 3430, second protein region 3432, first well region 3420, second well region 3422, interface accumulator 3460, check valve 3465, containment accumulator 3450, and/or check valve 3455.
  • interface plate 3520 provides fluid seals to regions 3420, 3422, 3430, 3432, and to accumulator 3450 and 3460.
  • interface plate 3520 provides one or more check valve actuators 3570 as best seen in Fig. 27B.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Micromachines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

Un appareil microfluide de matrice MxN pour réaliser une matrice de réactions, l'appareil ayant une pluralité de cellules de réaction en communication avec soit une entrée d'échantillon ou une entrée de réactif par une voie formée dans un bloc élastomère de l'appareil. Les méthodes fournies comprennent une méthode pour former des voies en parallèle dans une couche élastomère d'un bloc élastomère d'un dispositif microfluide, la méthode comprenant l'utilisation de masques photorésistants imprimés et de réactifs d'attaque pour détacher des zones ou des portions d'une couche élastomère du bloc élastomère.
PCT/US2005/015352 2004-05-02 2005-05-02 Dispositif de réaction thermique et méthode d'utilisation de semblable WO2005107938A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP05766055.7A EP1796824A4 (fr) 2004-05-02 2005-05-02 Dispositif de réaction thermique et méthode d'utilisation de semblable
CN2005800225837A CN1997454B (zh) 2004-05-02 2005-05-02 热反应设备及使用其的方法
JP2007511507A JP5502275B2 (ja) 2004-05-02 2005-05-02 熱反応デバイスおよびその熱反応デバイスの使用方法

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US10/837,885 US7476363B2 (en) 2003-04-03 2004-05-02 Microfluidic devices and methods of using same
US10/837,885 2004-05-02
US10/876,046 US20050145496A1 (en) 2003-04-03 2004-06-23 Thermal reaction device and method for using the same
US10/876,046 2004-06-23
US11/043,895 2005-01-25
US11/043,895 US8105553B2 (en) 2004-01-25 2005-01-25 Crystal forming devices and systems and methods for using the same
US11/058,106 2005-02-14
US11/058,106 US7867763B2 (en) 2004-01-25 2005-02-14 Integrated chip carriers with thermocycler interfaces and methods of using the same

Publications (2)

Publication Number Publication Date
WO2005107938A2 true WO2005107938A2 (fr) 2005-11-17
WO2005107938A3 WO2005107938A3 (fr) 2006-01-12

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JP (1) JP5502275B2 (fr)
WO (1) WO2005107938A2 (fr)

Cited By (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007189964A (ja) * 2006-01-20 2007-08-02 Toppan Printing Co Ltd 検査基板
US7307802B2 (en) 2004-06-07 2007-12-11 Fluidigm Corporation Optical lens system and method for microfluidic devices
WO2008089493A2 (fr) * 2007-01-19 2008-07-24 Fluidigm Corporation Dispositifs microfluidiques d'efficacité élevée et de précision élevée et procédés
JP2009042103A (ja) * 2007-08-09 2009-02-26 Sony Corp 基板、これを用いた反応処理装置並びに反応制御方法
WO2008049083A3 (fr) * 2006-10-18 2009-03-12 Harvard College Dosage biologique à écoulement latéral et écoulement traversant basé sur un support poreux à motif, ses procédés de fabrication et ses procédés d'utilisation
JP2010509918A (ja) * 2006-11-15 2010-04-02 アイダホ テクノロジー インコーポレーテッド 高密度内蔵型生物学的分析
EP2212437A1 (fr) * 2007-11-07 2010-08-04 The University Of British Columbia Dispositif microfluidique et procédé d'utilisation de ce dispositif
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WO2010112273A1 (fr) * 2009-03-31 2010-10-07 Siemens Aktiengesellschaft Dispositif et procédé pour recevoir un capteur
US7913928B2 (en) 2005-11-04 2011-03-29 Alliant Techsystems Inc. Adaptive structures, systems incorporating same and related methods
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US8206992B2 (en) 2008-03-27 2012-06-26 President And Fellows Of Harvard College Cotton thread as a low-cost multi-assay diagnostic platform
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WO2014020977A1 (fr) * 2012-08-03 2014-02-06 ソニー株式会社 Analyseur d'acide nucléique, micropuce pour l'analyse d'acide nucléique et procédé pour le montage d'une micropuce dans un dispositif
US8697363B2 (en) 2008-08-26 2014-04-15 Fluidigm Corporation Methods for detecting multiple target nucleic acids in multiple samples by use nucleotide tags
US8815162B2 (en) 2006-06-08 2014-08-26 Koninklijke Philips N.V. Microelectronic sensor device for DNA detection
US8821810B2 (en) 2010-02-03 2014-09-02 President And Fellows Of Harvard College Devices and methods for multiplexed assays
US8828661B2 (en) 2006-04-24 2014-09-09 Fluidigm Corporation Methods for detection and quantification of nucleic acid or protein targets in a sample
US8833430B2 (en) 2008-06-26 2014-09-16 President And Fellows Of Harvard College Versatile high aspect ratio actuatable nanostructured materials through replication
US8969014B2 (en) 2011-04-18 2015-03-03 Evitraproteoma Ab Methods for determining ligand binding to a target protein using a thermal shift assay
US9074204B2 (en) 2011-05-20 2015-07-07 Fluidigm Corporation Nucleic acid encoding reactions
US9102911B2 (en) 2009-05-15 2015-08-11 Biofire Diagnostics, Llc High density self-contained biological analysis
US9132426B2 (en) 2012-08-01 2015-09-15 Nanomdx, Inc. Simplified gating method for sealing and flow control in micro and nano devices
US9157116B2 (en) 2008-02-08 2015-10-13 Fluidigm Corporation Combinatorial amplification and detection of nucleic acids
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US9523693B2 (en) 2011-04-18 2016-12-20 Biotarget Engagement Interest Group Ab Methods for determining ligand binding to a target protein using a thermal shift assay
US9651568B2 (en) 2009-11-23 2017-05-16 Cyvek, Inc. Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays
US9700889B2 (en) 2009-11-23 2017-07-11 Cyvek, Inc. Methods and systems for manufacture of microarray assay systems, conducting microfluidic assays, and monitoring and scanning to obtain microfluidic assay results
US9759718B2 (en) 2009-11-23 2017-09-12 Cyvek, Inc. PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use
US9829488B2 (en) 2008-03-27 2017-11-28 President And Fellows Of Havard College Paper-based cellular arrays
US9840732B2 (en) 2012-05-21 2017-12-12 Fluidigm Corporation Single-particle analysis of particle populations
US9855735B2 (en) 2009-11-23 2018-01-02 Cyvek, Inc. Portable microfluidic assay devices and methods of manufacture and use
WO2018060326A1 (fr) 2016-09-29 2018-04-05 Qiagen Lake Constance Gmbh Agencement de récipient d'échantillon
US9952126B2 (en) 2012-02-29 2018-04-24 Fluidigm Corporation Methods for multiple single-cell capturing and processing using micro fluidics
US10065403B2 (en) 2009-11-23 2018-09-04 Cyvek, Inc. Microfluidic assay assemblies and methods of manufacture
CN109406442A (zh) * 2018-10-30 2019-03-01 桂林电子科技大学 一种快速测量蛋白质热变性温度的方法
US10228367B2 (en) 2015-12-01 2019-03-12 ProteinSimple Segmented multi-use automated assay cartridge
CN109982778A (zh) * 2016-09-23 2019-07-05 阿谢尔德克斯有限公司 流体系统及相关方法
EP3668648A4 (fr) * 2017-08-14 2021-05-05 Kimantech, L.L.C. Dispositif de distribution de liquide
US11117113B2 (en) 2015-12-16 2021-09-14 Fluidigm Corporation High-level multiplex amplification
US11707741B2 (en) 2005-05-09 2023-07-25 Biofire Diagnostics, Llc Self-contained biological analysis

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Publication number Priority date Publication date Assignee Title
US8921118B2 (en) 2008-03-27 2014-12-30 President And Fellows Of Harvard College Paper-based microfluidic systems
US8961902B2 (en) * 2008-04-23 2015-02-24 Bioscale, Inc. Method and apparatus for analyte processing
US9500645B2 (en) 2009-11-23 2016-11-22 Cyvek, Inc. Micro-tube particles for microfluidic assays and methods of manufacture
WO2013134742A2 (fr) 2012-03-08 2013-09-12 Cyvek, Inc Particules micro-tubulaires pour analyses microfluidiques et procédés de fabrication
CN102713621B (zh) 2009-11-23 2016-10-19 西维克公司 用于施行化验的方法和设备
WO2012129455A2 (fr) 2011-03-22 2012-09-27 Cyvek, Inc Dispositifs micro-fluidiques et leurs procédés de fabrication et d'utilisation
EP3116651B1 (fr) * 2014-03-11 2020-04-22 Illumina, Inc. Cartouche microfluidique intégrée jetable, et procédés pour la fabriquer
US9373561B1 (en) 2014-12-18 2016-06-21 International Business Machines Corporation Integrated circuit barrierless microfluidic channel
DK3100587T3 (da) * 2015-04-07 2020-10-12 Cell Id Pte Ltd Jævnstrømsvarmer
CN108474731B (zh) 2016-01-21 2021-09-21 东京毅力科创株式会社 异物检测装置和异物检测方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020037499A1 (en) * 2000-06-05 2002-03-28 California Institute Of Technology Integrated active flux microfluidic devices and methods
US20020064885A1 (en) * 2000-06-28 2002-05-30 William Bedingham Sample processing devices
US20030143120A1 (en) * 2002-01-25 2003-07-31 Waldemar Ruediger Parallel chemistry reactor with interchangeable vessel carrying inserts
US20030175947A1 (en) * 2001-11-05 2003-09-18 Liu Robin Hui Enhanced mixing in microfluidic devices
US20040141887A1 (en) * 2002-11-08 2004-07-22 Irm, Llc Apparatus and methods to process substrate surface features
US20050129581A1 (en) * 2003-04-03 2005-06-16 Fluidigm Corporation Microfluidic devices and methods of using same

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5241450A (en) * 1992-03-13 1993-08-31 The United States Of America As Represented By The United States Department Of Energy Three dimensional, multi-chip module
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
WO2001096880A1 (fr) * 2000-06-15 2001-12-20 Irm, Llc Support de precision automatique
US6508988B1 (en) * 2000-10-03 2003-01-21 California Institute Of Technology Combinatorial synthesis system
US6960437B2 (en) * 2001-04-06 2005-11-01 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
EP1442263A2 (fr) * 2001-10-29 2004-08-04 CHART HEAT EXCHANGERS Limited Partnership Echangeur thermique
EP1463796B1 (fr) * 2001-11-30 2013-01-09 Fluidigm Corporation Dispositif microfluidique et procedes d'utilisation de ce dernier
US7198759B2 (en) * 2002-07-26 2007-04-03 Applera Corporation Microfluidic devices, methods, and systems
US7452712B2 (en) * 2002-07-30 2008-11-18 Applied Biosystems Inc. Sample block apparatus and method of maintaining a microcard on a sample block

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020037499A1 (en) * 2000-06-05 2002-03-28 California Institute Of Technology Integrated active flux microfluidic devices and methods
US20020064885A1 (en) * 2000-06-28 2002-05-30 William Bedingham Sample processing devices
US20030175947A1 (en) * 2001-11-05 2003-09-18 Liu Robin Hui Enhanced mixing in microfluidic devices
US20030143120A1 (en) * 2002-01-25 2003-07-31 Waldemar Ruediger Parallel chemistry reactor with interchangeable vessel carrying inserts
US20040141887A1 (en) * 2002-11-08 2004-07-22 Irm, Llc Apparatus and methods to process substrate surface features
US20050129581A1 (en) * 2003-04-03 2005-06-16 Fluidigm Corporation Microfluidic devices and methods of using same

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US9234237B2 (en) 2004-06-07 2016-01-12 Fluidigm Corporation Optical lens system and method for microfluidic devices
US8048378B2 (en) 2004-06-07 2011-11-01 Fluidigm Corporation Optical lens system and method for microfluidic devices
US9663821B2 (en) 2004-06-07 2017-05-30 Fluidigm Corporation Optical lens system and method for microfluidic devices
US8512640B2 (en) 2004-06-07 2013-08-20 Fluidigm Corporation Optical lens system and method for microfluidic devices
US7906072B2 (en) 2004-06-07 2011-03-15 Fluidigm Corporation Optical lens system and method for microfluidic devices
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US8926905B2 (en) 2004-06-07 2015-01-06 Fluidigm Corporation Optical lens system and method for microfluidic devices
US10106846B2 (en) 2004-06-07 2018-10-23 Fluidigm Corporation Optical lens system and method for microfluidic devices
US7307802B2 (en) 2004-06-07 2007-12-11 Fluidigm Corporation Optical lens system and method for microfluidic devices
US11707741B2 (en) 2005-05-09 2023-07-25 Biofire Diagnostics, Llc Self-contained biological analysis
US7913928B2 (en) 2005-11-04 2011-03-29 Alliant Techsystems Inc. Adaptive structures, systems incorporating same and related methods
US8534570B2 (en) 2005-11-04 2013-09-17 Alliant Techsystems Inc. Adaptive structures, systems incorporating same and related methods
JP2007189964A (ja) * 2006-01-20 2007-08-02 Toppan Printing Co Ltd 検査基板
US8828661B2 (en) 2006-04-24 2014-09-09 Fluidigm Corporation Methods for detection and quantification of nucleic acid or protein targets in a sample
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US9644235B2 (en) 2006-04-24 2017-05-09 Fluidigm Corporation Methods for detection and quantification of nucleic acid or protein targets in a sample
US8815162B2 (en) 2006-06-08 2014-08-26 Koninklijke Philips N.V. Microelectronic sensor device for DNA detection
US8377710B2 (en) 2006-10-18 2013-02-19 President And Fellows Of Harvard College Lateral flow and flow-through bioassay devices based on patterned porous media, methods of making same, and methods of using same
WO2008049083A3 (fr) * 2006-10-18 2009-03-12 Harvard College Dosage biologique à écoulement latéral et écoulement traversant basé sur un support poreux à motif, ses procédés de fabrication et ses procédés d'utilisation
US9193988B2 (en) 2006-10-18 2015-11-24 President And Fellows Of Harvard College Lateral flow and flow-through bioassay devices based on patterned porous media, methods of making same, and methods of using same
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JP2010509918A (ja) * 2006-11-15 2010-04-02 アイダホ テクノロジー インコーポレーテッド 高密度内蔵型生物学的分析
US10053728B2 (en) 2006-11-15 2018-08-21 Biofire Diagnostics, Llc High density self-contained biological analysis
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US11866774B2 (en) 2006-11-15 2024-01-09 Biofire Diagnostics, Llc High density self-contained biological analysis
WO2008089493A3 (fr) * 2007-01-19 2008-11-20 Fluidigm Corp Dispositifs microfluidiques d'efficacité élevée et de précision élevée et procédés
WO2008089493A2 (fr) * 2007-01-19 2008-07-24 Fluidigm Corporation Dispositifs microfluidiques d'efficacité élevée et de précision élevée et procédés
US8157434B2 (en) 2007-01-19 2012-04-17 Fluidigm Corporation High efficiency and high precision microfluidic devices and methods
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US9211537B2 (en) 2007-11-07 2015-12-15 The University Of British Columbia Microfluidic device and method of using same
EP2212437A4 (fr) * 2007-11-07 2011-09-28 Univ British Columbia Dispositif microfluidique et procédé d'utilisation de ce dispositif
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US9157116B2 (en) 2008-02-08 2015-10-13 Fluidigm Corporation Combinatorial amplification and detection of nucleic acids
US8628729B2 (en) 2008-03-27 2014-01-14 President And Fellows Of Harvard College Three-dimensional microfluidic devices
US9829488B2 (en) 2008-03-27 2017-11-28 President And Fellows Of Havard College Paper-based cellular arrays
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US8697363B2 (en) 2008-08-26 2014-04-15 Fluidigm Corporation Methods for detecting multiple target nucleic acids in multiple samples by use nucleotide tags
CN104741159A (zh) * 2008-12-08 2015-07-01 富鲁达公司 可编程微流体数字阵列
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US8921049B2 (en) 2009-01-28 2014-12-30 Fluidigm Corporation Determination of copy number differences by amplification
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US9192933B2 (en) 2009-03-06 2015-11-24 President And Fellows Of Harvard College Microfluidic, electrochemical devices
WO2010112273A1 (fr) * 2009-03-31 2010-10-07 Siemens Aktiengesellschaft Dispositif et procédé pour recevoir un capteur
WO2010115154A1 (fr) 2009-04-02 2010-10-07 Fluidigm Corporation Procédé d'amplification à amorce multiple pour codage à barres d'acides nucléiques cibles
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US8691509B2 (en) 2009-04-02 2014-04-08 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
EP2789694A1 (fr) 2009-04-02 2014-10-15 Fluidigm Corporation Dispositif microfluidique avec système de récupération de produit de réaction
US10344318B2 (en) 2009-04-02 2019-07-09 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
US11795494B2 (en) 2009-04-02 2023-10-24 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
US9677119B2 (en) 2009-04-02 2017-06-13 Fluidigm Corporation Multi-primer amplification method for tagging of target nucleic acids
US9102911B2 (en) 2009-05-15 2015-08-11 Biofire Diagnostics, Llc High density self-contained biological analysis
US8551787B2 (en) 2009-07-23 2013-10-08 Fluidigm Corporation Microfluidic devices and methods for binary mixing
US9651568B2 (en) 2009-11-23 2017-05-16 Cyvek, Inc. Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays
US9855735B2 (en) 2009-11-23 2018-01-02 Cyvek, Inc. Portable microfluidic assay devices and methods of manufacture and use
US9700889B2 (en) 2009-11-23 2017-07-11 Cyvek, Inc. Methods and systems for manufacture of microarray assay systems, conducting microfluidic assays, and monitoring and scanning to obtain microfluidic assay results
US9759718B2 (en) 2009-11-23 2017-09-12 Cyvek, Inc. PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use
US10022696B2 (en) 2009-11-23 2018-07-17 Cyvek, Inc. Microfluidic assay systems employing micro-particles and methods of manufacture
US10065403B2 (en) 2009-11-23 2018-09-04 Cyvek, Inc. Microfluidic assay assemblies and methods of manufacture
US8821810B2 (en) 2010-02-03 2014-09-02 President And Fellows Of Harvard College Devices and methods for multiplexed assays
US9528996B2 (en) 2011-04-18 2016-12-27 Biotarget Engagement Interest Group Ab Methods for determining ligand binding to a target protein using a thermal shift assay
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US8969014B2 (en) 2011-04-18 2015-03-03 Evitraproteoma Ab Methods for determining ligand binding to a target protein using a thermal shift assay
WO2012154876A1 (fr) 2011-05-09 2012-11-15 Fluidigm Corporation Détection d'acide nucléique à l'aide d'une sonde
US10501786B2 (en) 2011-05-20 2019-12-10 Fluidigm Corporation Nucleic acid encoding reactions
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US9074204B2 (en) 2011-05-20 2015-07-07 Fluidigm Corporation Nucleic acid encoding reactions
CN102320558A (zh) * 2011-09-13 2012-01-18 上海先进半导体制造股份有限公司 全硅基微流体器件的腔体的制造方法
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US9952126B2 (en) 2012-02-29 2018-04-24 Fluidigm Corporation Methods for multiple single-cell capturing and processing using micro fluidics
US9840732B2 (en) 2012-05-21 2017-12-12 Fluidigm Corporation Single-particle analysis of particle populations
US9132426B2 (en) 2012-08-01 2015-09-15 Nanomdx, Inc. Simplified gating method for sealing and flow control in micro and nano devices
JPWO2014020977A1 (ja) * 2012-08-03 2016-07-21 ソニー株式会社 核酸分析装置、核酸分析用マイクロチップ及び該装置におけるマイクロチップ搭載方法
US9925540B2 (en) 2012-08-03 2018-03-27 Sony Corporation Nucleic acid analysis apparatus, microchip for nucleic acid analysis, and method for mounting microchip in nucleic acid analysis apparatus
WO2014020977A1 (fr) * 2012-08-03 2014-02-06 ソニー株式会社 Analyseur d'acide nucléique, micropuce pour l'analyse d'acide nucléique et procédé pour le montage d'une micropuce dans un dispositif
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US11117113B2 (en) 2015-12-16 2021-09-14 Fluidigm Corporation High-level multiplex amplification
US11857940B2 (en) 2015-12-16 2024-01-02 Fluidigm Corporation High-level multiplex amplification
EP3515601A4 (fr) * 2016-09-23 2020-06-10 Archerdx, Inc. Système fluidique et procédés associés
CN109982778A (zh) * 2016-09-23 2019-07-05 阿谢尔德克斯有限公司 流体系统及相关方法
WO2018060326A1 (fr) 2016-09-29 2018-04-05 Qiagen Lake Constance Gmbh Agencement de récipient d'échantillon
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