WO2005107938A3 - Dispositif de réaction thermique et méthode d'utilisation de semblable - Google Patents

Dispositif de réaction thermique et méthode d'utilisation de semblable Download PDF

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Publication number
WO2005107938A3
WO2005107938A3 PCT/US2005/015352 US2005015352W WO2005107938A3 WO 2005107938 A3 WO2005107938 A3 WO 2005107938A3 US 2005015352 W US2005015352 W US 2005015352W WO 2005107938 A3 WO2005107938 A3 WO 2005107938A3
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WO
WIPO (PCT)
Prior art keywords
elastomeric
same
reaction device
thermal reaction
elastomeric block
Prior art date
Application number
PCT/US2005/015352
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English (en)
Other versions
WO2005107938A2 (fr
Inventor
Federico Goodsaid
Marc A Unger
Jiang Huang
Emerson Quan
Robert Grossman
Phillip Lam
Hou-Pu Chou
Jake Kimball
Martin Pieprzyk
Geoffrey Facer
Original Assignee
Fluidigm Corp
Federico Goodsaid
Marc A Unger
Jiang Huang
Emerson Quan
Robert Grossman
Phillip Lam
Hou-Pu Chou
Jake Kimball
Martin Pieprzyk
Geoffrey Facer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/837,885 external-priority patent/US7476363B2/en
Priority claimed from US10/876,046 external-priority patent/US20050145496A1/en
Priority claimed from US11/043,895 external-priority patent/US8105553B2/en
Priority claimed from US11/058,106 external-priority patent/US7867763B2/en
Application filed by Fluidigm Corp, Federico Goodsaid, Marc A Unger, Jiang Huang, Emerson Quan, Robert Grossman, Phillip Lam, Hou-Pu Chou, Jake Kimball, Martin Pieprzyk, Geoffrey Facer filed Critical Fluidigm Corp
Priority to EP05766055.7A priority Critical patent/EP1796824A4/fr
Priority to CN2005800225837A priority patent/CN1997454B/zh
Priority to JP2007511507A priority patent/JP5502275B2/ja
Publication of WO2005107938A2 publication Critical patent/WO2005107938A2/fr
Publication of WO2005107938A3 publication Critical patent/WO2005107938A3/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/527—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for microfluidic devices, e.g. used for lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00353—Pumps
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00389—Feeding through valves
    • B01J2219/004—Pinch valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00389—Feeding through valves
    • B01J2219/004—Pinch valves
    • B01J2219/00403—Pinch valves in multiple arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00495—Means for heating or cooling the reaction vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00572—Chemical means
    • B01J2219/00576—Chemical means fluorophore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B01J2219/00587—High throughput processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0638—Valves, specific forms thereof with moving parts membrane valves, flap valves

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Micromachines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un appareil microfluide de matrice MxN pour réaliser une matrice de réactions, l'appareil ayant une pluralité de cellules de réaction en communication avec soit une entrée d'échantillon ou une entrée de réactif par une voie formée dans un bloc élastomère de l'appareil. Les méthodes fournies comprennent une méthode pour former des voies en parallèle dans une couche élastomère d'un bloc élastomère d'un dispositif microfluide, la méthode comprenant l'utilisation de masques photorésistants imprimés et de réactifs d'attaque pour détacher des zones ou des portions d'une couche élastomère du bloc élastomère.
PCT/US2005/015352 2004-05-02 2005-05-02 Dispositif de réaction thermique et méthode d'utilisation de semblable WO2005107938A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP05766055.7A EP1796824A4 (fr) 2004-05-02 2005-05-02 Dispositif de réaction thermique et méthode d'utilisation de semblable
CN2005800225837A CN1997454B (zh) 2004-05-02 2005-05-02 热反应设备及使用其的方法
JP2007511507A JP5502275B2 (ja) 2004-05-02 2005-05-02 熱反応デバイスおよびその熱反応デバイスの使用方法

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US10/837,885 2004-05-02
US10/837,885 US7476363B2 (en) 2003-04-03 2004-05-02 Microfluidic devices and methods of using same
US10/876,046 2004-06-23
US10/876,046 US20050145496A1 (en) 2003-04-03 2004-06-23 Thermal reaction device and method for using the same
US11/043,895 US8105553B2 (en) 2004-01-25 2005-01-25 Crystal forming devices and systems and methods for using the same
US11/043,895 2005-01-25
US11/058,106 US7867763B2 (en) 2004-01-25 2005-02-14 Integrated chip carriers with thermocycler interfaces and methods of using the same
US11/058,106 2005-02-14

Publications (2)

Publication Number Publication Date
WO2005107938A2 WO2005107938A2 (fr) 2005-11-17
WO2005107938A3 true WO2005107938A3 (fr) 2006-01-12

Family

ID=35320754

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/015352 WO2005107938A2 (fr) 2004-05-02 2005-05-02 Dispositif de réaction thermique et méthode d'utilisation de semblable

Country Status (3)

Country Link
EP (1) EP1796824A4 (fr)
JP (1) JP5502275B2 (fr)
WO (1) WO2005107938A2 (fr)

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US8921118B2 (en) 2008-03-27 2014-12-30 President And Fellows Of Harvard College Paper-based microfluidic systems
US9216412B2 (en) 2009-11-23 2015-12-22 Cyvek, Inc. Microfluidic devices and methods of manufacture and use
US9229001B2 (en) 2009-11-23 2016-01-05 Cyvek, Inc. Method and apparatus for performing assays
US9500645B2 (en) 2009-11-23 2016-11-22 Cyvek, Inc. Micro-tube particles for microfluidic assays and methods of manufacture
US9546932B2 (en) 2009-11-23 2017-01-17 Cyvek, Inc. Microfluidic assay operating system and methods of use

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CA2607661C (fr) 2005-05-09 2017-08-08 Idaho Technology, Inc. Appareil et methode d'amplification d'acide nucleique en deux etapes
US7913928B2 (en) 2005-11-04 2011-03-29 Alliant Techsystems Inc. Adaptive structures, systems incorporating same and related methods
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AU2007310987B2 (en) * 2006-10-18 2014-01-09 President And Fellows Of Harvard College Lateral flow and flow-through bioassay devices based on patterned porous media, methods of making same, and methods of using same
CN101578520B (zh) 2006-10-18 2015-09-16 哈佛学院院长等 基于形成图案的多孔介质的横向流动和穿过生物测定装置、及其制备方法和使用方法
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EP2212437A4 (fr) * 2007-11-07 2011-09-28 Univ British Columbia Dispositif microfluidique et procédé d'utilisation de ce dispositif
US9157116B2 (en) 2008-02-08 2015-10-13 Fluidigm Corporation Combinatorial amplification and detection of nucleic acids
KR101510785B1 (ko) 2008-03-27 2015-04-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 저렴한 멀티 검사 진단 플랫폼으로서의 무명실
WO2009120963A2 (fr) 2008-03-27 2009-10-01 President And Fellows Of Harvard College Réseaux cellulaires à base de papier
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EP2326732A4 (fr) 2008-08-26 2012-11-14 Fluidigm Corp Procédés de dosage pour un débit amélioré d'échantillons et/ou de cibles
CN104741159B (zh) * 2008-12-08 2017-01-18 富鲁达公司 可编程微流体数字阵列
US8450063B2 (en) 2009-01-28 2013-05-28 Fluidigm Corporation Determination of copy number differences by amplification
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DE102009015739A1 (de) * 2009-03-31 2010-10-07 Siemens Aktiengesellschaft Vorrichtung und Verfahren zur Aufnahme eines Sensors
CN103952482A (zh) 2009-04-02 2014-07-30 弗卢伊蒂格姆公司 用于对目标核酸进行条形码化的多引物扩增方法
US8551787B2 (en) 2009-07-23 2013-10-08 Fluidigm Corporation Microfluidic devices and methods for binary mixing
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US10065403B2 (en) 2009-11-23 2018-09-04 Cyvek, Inc. Microfluidic assay assemblies and methods of manufacture
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US9700889B2 (en) 2009-11-23 2017-07-11 Cyvek, Inc. Methods and systems for manufacture of microarray assay systems, conducting microfluidic assays, and monitoring and scanning to obtain microfluidic assay results
US9759718B2 (en) 2009-11-23 2017-09-12 Cyvek, Inc. PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use
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US9523693B2 (en) 2011-04-18 2016-12-20 Biotarget Engagement Interest Group Ab Methods for determining ligand binding to a target protein using a thermal shift assay
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CN102320558B (zh) * 2011-09-13 2014-03-26 上海先进半导体制造股份有限公司 全硅基微流体器件的腔体的制造方法
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EP1796824A4 (fr) 2016-10-19
JP2007537847A (ja) 2007-12-27
WO2005107938A2 (fr) 2005-11-17
EP1796824A2 (fr) 2007-06-20
JP5502275B2 (ja) 2014-05-28

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