WO2005104853A1 - 植物病害の発生を抑制する微生物農薬 - Google Patents
植物病害の発生を抑制する微生物農薬 Download PDFInfo
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- WO2005104853A1 WO2005104853A1 PCT/JP2005/007981 JP2005007981W WO2005104853A1 WO 2005104853 A1 WO2005104853 A1 WO 2005104853A1 JP 2005007981 W JP2005007981 W JP 2005007981W WO 2005104853 A1 WO2005104853 A1 WO 2005104853A1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Definitions
- Microbial pesticides that suppress the occurrence of plant diseases
- the present invention relates to a microbial pesticide that suppresses the occurrence of plant diseases, more specifically, a microbial pesticide containing the insect-parasitic fungus vertatisillium le reii- (Verticillium lecanii), and the microbial pesticide such as fruits and vegetables.
- the present invention relates to a microbial pesticide which is applied to a plant or applied to a plant cultivation soil to exert an effect of suppressing the growth of a plant disease bacterium and improving the soil.
- Control of plant pests such as vegetables, fruits, rice, wheat, and fruits, or control of soil pests attached to the rhizome of plants has been developed with the use of chemicals.
- Control with insecticides and fungicides is dominant.
- pesticides using chemical substances have excellent effects, some of them are toxic to humans and livestock, and others remain in the natural environment and affect other ecosystems.
- insects such as the emergence of resistant pests by long-term use, and the emergence of pests in an environment where pests are generated by killing natural enemies that originally existed. I have a problem. In such a situation, microbial pesticides are expected as a means to solve the problem.
- Microorganisms that can be used as microbial pesticides include non-pathogenic Fusarium which activates the resistance originally held by plants to control diseases (for example, Non-Patent Document 1, Patent Reference 1), and Trichoderma that exhibits antibiotic action against pathogenic bacteria (for example, see Non-patent Reference 2) have been reported.
- a method for controlling plant diseases using Pseudomonas genus bacteria for example, bacterial wilt of a Solanaceae plant (see, for example, Patent Documents 2, 3, and 4), rice seedling wilt (for example, Reference 5), plant diseases caused by plant pathogens of the genus Potrichtis and Basylium (see, for example, Patent Document 6), plant diseases caused by Gram-positive bacteria (see, for example, Patent Document 7), and Fusarium spp.
- Methods for controlling plant diseases caused by the disease for example, see Patent Document 8.
- a strain that can be controlled by Pseudomonas spp. A strain having antibacterial properties against phytopathogenic fungi is provided.
- Domonas sp. CGF7 strain for example, see Patent Document 9
- Pseudomonas' SP CGF878 strain as a microbial pesticide as an active ingredient
- Pseudomonas fluorescens bacteria in plants for example, A method of controlling rot caused by Pseudomonas cichorii as a pathogenic bacterium (see, for example, Patent Document 11), and inoculating solanaceous plants with Pseudomonas alcaligenes bacteria.
- a method for cultivating a solanaceous plant comprising the same for example, see Patent Document 12 is known.
- microorganisms having an antibacterial activity against grasses in particular, bacteria belonging to any of the genus Pseudomonas, Bacillus, or Enterobacter, as bacteria having an antagonistic action against bacterial diseases that occur during their seedling raising.
- Pesticides for example, see Patent Document 13
- bacteria of the genus Coniothyrium and sporidesmium have an effect on sclerotium disease
- bacteria of the genus Penicillium have an effect of controlling vertiyllium disease (for example, see Non-Patent Document 3).
- Trichoderma spp To control tobacco white silk, and the use of Trichoderma spp.
- Agrobacterium's radiopacter (batterose) is a fungicide composed of live cultures of 84 strains of Agrobacterium radiopacter, an antagonistic microorganism against root-cancer pathogens, and is the root of chrysanthemum and rose seedlings.
- Non-pathogenic Erbila's caratobola (biokeeper) is a microbial pesticide that is effective in the control of Chinese cabbage soft rot. Registered. It is known that a bacillus subtilis agent (botokiller) also registered has a protective effect against Botrytis cinerea on plants (for example, see Non-Patent Document 5).
- plant pesticides that control plant pests using entomopathogenic fungi have already been registered and have been published.
- microbial pesticides that uses Verti-Silium®, which is sold under trade names such as “Batarec” and “Mycotal”.
- the filamentous fungus verti-slime was first found as a parasitic bacterium of the scale insects, and has subsequently been isolated from a variety of insects and mites, such as aphids, whiteflies, and thrips.
- Microbial pesticides using verti-sirim are effective against aphids (Hemiptera), whiteflies (Hemiptera), and thrips (Hymenoptera) and wild mites, and in particular, various pests. It is expected to be used in greenhouse horticulture where the development of drug resistance is getting worse.
- a microbial pesticide using the verti-slime is formulated in the form of a wettable powder, and when used, dissolved in water and sprayed.
- the formulation contains spores of the verti-sirime, which, when sprayed and brought into contact with the pests, spores germinate and parasitize the pests, after which hyphae grow and fill in the pest's body. And cause the pests to die.
- Conventional strains of vertecilium 're-force' have low colonization ability on plant surfaces such as leaves, and if the microbial agent does not directly contact the surface of the pest, the parasitic ability will be lost.
- the present inventors have conducted extensive searches for strains of the filamentous fungus verticulture, which has the ability to colonize the surface of a plant such as a leaf surface. In addition, in terms of its ability to cause pathogenicity against pests, it has succeeded in isolating a sterile strain that is inferior to the conventional strain of Verteille slime.
- Verti-Silium'R-A- 2 strains MAFF238426
- Verticillium'-R-B- 2 strains MAFF238429
- Verti-Silium-R-R-C 1 strain MAFF238430
- Patent Document 1 Japanese Patent Publication No. 7-096485
- Patent Document 2 JP-A-6-9325
- Patent Document 3 JP-A-6-86668
- Patent Document 4 Japanese Patent Publication No. 6-17291
- Patent Document 5 JP-A-4-104783
- Patent Document 7 JP-A-5-310521
- Patent Document 8 Japanese Patent Publication No. 6-6523
- Patent Document 9 JP-A-09-255513
- Patent Document 10 JP-A-11-187866
- Patent Document 11 Japanese Patent Application Laid-Open No. 2001-247423
- Patent Document 12 Japanese Patent Application Laid-Open No. 2002-233246
- Patent Document 13 Relisted 99Z016859
- Patent Document 14 Japanese Patent Application Laid-Open No. 06-024925
- Patent Document 15 Japanese Patent Application Laid-Open No. 08-225419
- Patent Document 16 JP 2003-335612 A
- Non-Patent Document 1 Baker, Hanky, Doteller: Fight Pathology (Baker, Hanchey,
- Non-patent document 2 Ministry of Agriculture, Forestry and Fisheries registration No. 7023
- Non-patent document 3 Bio-pesticides and growth regulator development and use, LIC
- Non-Patent Document 4 Plant Pathology, 38, 227, 1989
- Non-Patent Document 5 "Agricultural Chemicals Notebook 2001 Edition", p. 352-353, published by the 11th edition of the Japan Plant Protection Association on November 1, 2001
- Non-patent document 6 ⁇ Agriculture of the month '' 2001, September issue, P72-77
- An object of the present invention is to control a plant disease by spraying a microbial pesticide containing a filamentous fungus vertiyllium 'recani, which suppresses the growth of plant pathogens, on fruits, vegetables, and the like. Applying a microbial pesticide or a microbial pesticide containing a filamentous fungus Verti-Silium® to control soil diseases instead of the conventional chemical, chlorpicrine (methyl bromide), which is a soil disinfectant
- An object of the present invention is to provide a soil conditioner having a soil conditioner effect.
- the present inventors have applied a fungal fungus Vertisilium's ability having a plant body surface-fixing ability to a plant as a microbial pesticide, thereby achieving a high inhibitory effect against pests. Although it was reported that the fungus had a beneficial effect, further studies were conducted to take advantage of the usefulness of the filamentous fungus, and it was found that it has an effect of remarkably inhibiting the growth of not only pests on plants but also plant pathogens. I found it.
- the present invention provides (1) a microbial pesticide comprising, as an active ingredient, a strain of Verticillium lecanii having a growth-inhibiting effect on plant pathogenic bacteria; 2) The above-mentioned (1), wherein the strain of Verteilium 'recanii is a strain having a plant surface settlement ability, and a strain having Z or a plant root surface and Z or rhizosphere settlement ability.
- Microorganism pesticides (3) strains having the ability to colonize plant surfaces, and strains having Z or the ability to colonize the root surface of plants and Z or rhizospheres are strains of Verti-Silium-Re-A-2 (MAFF238426 (2) a bacterial strain having the ability to settle on the plant surface, and Z or a bacterial strain having a root surface and the ability to colonize the root of the plant or Z or the rhizosphere.
- 'Recani B-2 MAFF2 (38429)
- a microbial pesticide according to (2) (5) a strain having a plant surface fixation ability, and a Z or a strain having a plant root surface and a Z or rhizosphere fixation ability.
- microorganism pesticide according to the above (2) wherein the microorganism is a vegetable or fruit.
- a microbial pesticide according to the above (1) to (5) which is a powdery mildew fungus, a gray mold fungus, a vine wilt fungus, or a half-body wilt fungus; and (7) strawberry gray mold fungus.
- a method for controlling plant diseases which comprises spraying the microbial pesticide described on the leaf surface of the plant.
- the present invention also provides (9) a soil pesticide-controlling microbial pesticide comprising as an active ingredient a strain of Verticillium lecanii which has a growth-inhibiting effect on soil-disease, 10) The above-mentioned (B), wherein the strain of Verticillium re-Rinji is a strain having an ability to adhere to the plant body surface and a strain having Z or the ability to colonize the root surface of the plant body and Z or in the rhizosphere. (9) a microbial pesticide described in (9), (11) a strain having a plant surface fixation ability, and Z or a strain having a plant root surface and Z or rhizosphere fixation ability.
- the present invention relates to a method for controlling a soil disease, which comprises applying a pesticide to the vicinity of a root of a plant.
- the present invention provides (18) a soil conditioner comprising, as a main component, the microbial pesticide for controlling a soil disease according to any of (9) to (16); ).
- a soil improvement method characterized by applying the soil improvement agent described in (1) to soil.
- FIG. 1 is a view showing the results of inhibition assay by biphasic culture of a strain of Verteilium's strain -4 and a strawberry gray mold fungus.
- FIG. 2 is a graph showing the degree of strawberry gray mold erosion after bacterial dispersal in a laboratory.
- FIG. 3 is a graph showing the results of the incidence of strawberry gray mold disease after inoculation of a bacterial strain in a field.
- Fig. 4 is a graph showing the adherence of a verti-silium of the present invention in soil infected with strawberry gray mold by cfu number.
- FIG. 5 is a graph showing the melon root surface fixability of the verteilium 'recanii' of the present invention in terms of cfu number.
- Fig. 6 is a graph showing the fixability of the vertical slime of the present invention in the melon rhizosphere by the number of cfu.
- FIG. 7 is a graph showing the inhibitory action of the verti-sirime's force of the present invention on the development of melon vine scab in the form of a symptom severity index (DSI).
- DSI symptom severity index
- Fig. 8 is a graph showing the inhibitory effect of the partysilium's force of the present invention on the development of melon vine scab by the weight of a living plant.
- Fig. 9 is a graph showing the colonization property of the vertecilium 'reiki- of the present invention in tomato wilt disease infected soil by cfu number.
- FIG. 10 is a view showing the tomato root surface fixability of the vertecilium 'recanii' of the present invention by the number of cfu.
- Fig. 11 is a graph showing the tomato rhizosphere-fixing ability of the vertical series "Reiki-" of the present invention by the number of cfu.
- FIG. 12 is a graph showing the action of suppressing the onset of tomato half-blight wilt of partysilium 'reiki-' of the present invention by appearance symptom index.
- Fig. 13 is a graph showing the onset inhibitory effect of Partisirium 'Recanii' of the present invention on tomato half-blight wilt in terms of internal symptom.
- Fig. 14 is a graph showing the inhibitory effect of the partysilium 'reiki-' of the present invention on tomato wilt of tomato in terms of plant body weight.
- FIG. 15 is a graph showing the inhibitory effect of the partysilium 're-force' of the present invention on tomato wilt of tomato in terms of dry plant weight.
- FIG. 16 is a graph showing, as a symptom index, a change in symptom 2 to 4 weeks after inoculation of the powdery mildew fungus with the inhibitory action of the particillium 'reiki-' of the present invention on powdery mildew.
- FIG. 17 The effect of the present invention of inhibiting the occurrence of powdery mildew of Cucumber powdery mildew in the control group and the strain of Verti-Silium® strain B-2 (50 ml treatment group) after 3 weeks. It is a photograph which shows the disease status of three leaves.
- the microbial pesticide of the present invention is not particularly limited as long as it is a microbial pesticide containing, as an active ingredient, a strain of vertical syrup 're-force', which has a growth inhibitory action against plant pathogenic bacteria.
- the disease control method is not particularly limited as long as it is a method of spraying a powerful microbial pesticide of the present invention on the foliage of a plant, and the microbial pesticide for soil disease control of the present invention includes A microbial pesticide comprising a strain of Verticillium having a growth-inhibiting effect as an active ingredient is not particularly limited, and the method for controlling soil disease of the present invention includes the viable microbial pesticide for controlling soil disease of the present invention.
- the above-mentioned plant pathogens which are not particularly limited as long as the method is applied near the roots of the plant include the powdery mildew fungi on vegetables such as cucumber powdery mildew, melon powdery mildew, etc. Vegetables such as gray mold fungi In addition to gray mold fungi on fruits, scab fungus, take-all fungus, sclerotium fungus, and white silk fungus can be mentioned.
- the strains of the above Verticillium re-power include A-1 strain, A-2 strain (MAFF238426), B-1 strain, B-2 strain (MAFF238429), C-1 strain (MAFF238430), C—2 shares, ATC C22611, ATCC22612, ATCC58907, ATCC58908, ATCC5890 9, MAFF235690, MAFF235693, MAFF235694, MAFF235696, MAFF235699, MAFF235701 and the like.
- the strain be a strain having the ability to settle on the surface of a plant body, or a strain having the ability to settle on the root surface of the plant body and Z or in the rhizosphere.
- the term "fixing ability on the surface of the plant” means the ability of the strain to settle on the surface of the plant for a predetermined period when the strain is sprayed on the surface of the plant.
- strains of the vertical series can be obtained from the Agricultural Biological Resources Genebank of the National Institute for Agrobiological Resources and the American Type Culture Collection.
- strain B-2 (MAFF238429) is useful against strawberry gray mold.
- Strawberry gray mold is an important strawberry disease caused by the pathogen Botrytis cinerea.
- chemical pesticides have been used to control the disease, but as a result, phytotoxic bacteria have emerged one after another, and are now considered as incurable diseases.
- the A-2 strain (MAFF238426) is useful for soil diseases caused by melon vine wilt.
- Melon vine scab is one of the most common diseases in greenhouse cultivation.
- FOM Field oxysporum f.sp.melonis
- the pathogen inhabits the soil and forms chlamydospores, for example, even in unfavorable environments, and remains at risk for disease for 4-5 years until the environment is favorable.
- chemical pesticides and resistant varieties are used to prevent this disease, they have the problem that new resistant pathogens quickly develop.
- the C-1 strain (MAFF238430) is useful against soil diseases caused by tomato wilt fungus.
- the lower leaves are partially withered and the edges of the leaves are curled up due to soil contamination by a fungus (Verticillium dahliae vdt_2).
- the withered part It yellows and the symptoms spread to the veins.
- the lower leaves gradually die. Discoloration of the conduit is seen but not very noticeable. It occurs regardless of open-field cultivation or greenhouse cultivation, and occurs more frequently at low temperatures and high humidity than under the conditions of vine disease.
- chemical pesticides and resistant varieties are used as countermeasures for this disease, but these have a problem if new resistant pathogens soon emerge.
- the strain of Verticillium 'Renji' of the present invention as a microbial pesticide or a microbial pesticide for controlling soil disease, it is necessary to use a method used for preparing a general microbial pesticide.
- a method used for preparing a general microbial pesticide can.
- cells containing spores and the like are appropriately combined with mineral carriers such as celite and kaolin, saccharides such as starch, sucrose, glucose, and surfactants to form powders, which are dissolved in water before use. It can be formulated in the form of a dispersible, wettable powder.
- polyoxyethylene alkyl ethers and esters polyoxyethylene alkyl phenyl ethers and esters, polyoxyethylene fatty acid esters, and polyoxyethylene sorbitan fatty acid esters are used. It is possible to improve the adhesion.
- the microbial pesticide of the present invention uses a strain of Verticillium vulgaris, which has a settling property on the surface of a plant such as a leaf, as a control agent for a pathogenic fungus of a plant.
- Verticillium vulgaris which has a settling property on the surface of a plant such as a leaf
- the microbial pesticide of the present invention can be sprayed before a disease caused by a plant pathogenic bacterium occurs. It is no longer necessary to spray every time an outbreak occurs.
- the microbial pesticide of the present invention when applied to a plant cultivation pot, it is established on the root surface or rhizosphere of the plant, and then transplanted to soil contaminated with pathogenic bacteria (Fusarium or Verticillium). Fusarium disease,
- Verticillium disease Therefore, the frequency of application can be reduced as compared with the conventional one, the cost and labor in plant disease control can be reduced, and plant and soil diseases can be simultaneously controlled.
- the conventional method after the disease caused by plant pathogens, it was necessary to apply it every two weeks. If the microbial pesticide of the present invention was applied once, it could be further applied during the plant cultivation period. No need to The The rhizosphere is the area around the root of the plant, about 3mm to 5mm. This area is the area of contact between the soil and the plant, and is extremely strongly affected by both plant power and soil.
- the soil conditioner of the present invention may be any as long as it contains the microbial pesticide for controlling soil diseases of the present invention as a main component. There is no particular limitation as long as the method for applying the soil improver of the present invention to soil is used.
- the soil conditioner of the present invention can be applied before or after contamination with soil disease bacteria, and can control soil disease caused by soil disease bacteria.
- Example 1 biocontrol of strawberry gray mold by Verti-Silium Recanii
- Example 2 bio-control of Melon Fusarium wilt by Verti-Silium cv.
- the biocontrol of tomato half-blight wilt by 'Re-power' will be explained respectively.
- the antagonism of vertebrate slime against the strawberry gray mold fungus was examined on the medium.
- the Verti-Silium-Ren-4 strain was tested by dual culture with strawberry gray mold.
- An agar disk of strawberry gray mold fungus is placed on one side of a potato dextrose agar (PDA: Sigma) plate, and the other side of the same plate is cultivated on a potato sucrose agar (PSA).
- PDA potato dextrose agar
- PSA potato sucrose agar
- the agar disk was placed. After incubation at 25 ° C for 5 days, the inhibition zone width (mm) was measured. The same experiment was performed three times. Found between the verti-serum and the strawberry gray mold fungus Hyphae interactions were observed under an Olympus light microscope.
- Healthy strawberry fruits were surface-sterilized with 5% antiformin for 5 minutes and washed with distilled water. These strawberry fruits were added as antagonists with a spore suspension (1 ⁇ 10 7 spores / ml) of a strain of Bertidium 'Re-A-2 and B-2, and a spore suspension of strawberry gray mold (l X 10 6 spor es Zml) and was sprayed with. Ten fruits were used per treatment. Fruits sprayed with distilled water were used as controls. Tween 80 was added as a surfactant to the above suspension and distilled water. The treated fruits were kept in an incubator at 25 ° C.
- the gray mold index when the fruit was treated with the Verti-Silium 'Re-force' was 0.9-1.0 (Fig. 2). No significant difference was observed between the results obtained with all strains and the results obtained with distilled water. Although the gray mold index of fruits treated with the Verti-Silium 'Re-force' was low, these fruits were covered by other molds.
- the strain of Verti-Silium'R-B-2 has the best antagonistic properties (FIG. 3).
- the B-2 strain suppressed the incidence of gray mold to 5.0%, and was shown to be more effective than Notalec and Mycotal.
- the correlation between the average temperature and relative humidity of Obihiro and the average value of gray mold incidence during the experiment was examined, the incidence of strawberry gray mold was correlated with the relative humidity.
- Verticillium-lechini listed in Table 1, including commercially available Batarec and mycotal, were tested. Incubate the powerful fungi in the dark at 25 ⁇ 1 ° C for 2 weeks on potato scrosquitin agar (PSCA: 200 g potato per liter, 20 g sucrose, 10 g chitin, 20 g agar) plates, and then place in humid These strains were grown on horticultural soil and bran (4: 1, w Zw) for 2 weeks. On the other hand, Melon Fusarium vine fungus (FOM) was isolated from the melon fruit tree at the Tokachi Agricultural Experiment Station. Virulent pathogens were maintained and grown on potato dextrose agar (PDA) at 5 ⁇ 1 ° C.
- PDA potato dextrose agar
- the antagonism of Vertidium reticulatum against Melon Fusarium wilt fungus was examined on the medium. Twenty strains of Verteilium'recanii were tested by dual culture with Melon Fusarium vine wilt. Melon Fusarium Vine Crack Place the agar disk of the bacterial pathogen on one side of a potato dextrose agar (PDA: Sigma) plate, and on the other side of the same plate, cultivate the bait-serum cultivated on potato sucrose agar (PSA). A gar disk was placed. After culturing at 25 ° C for 10 days, the band width (mm) was measured. The same experiment was performed three times. Hyphal interactions observed between the vertebrate film and the melonfusarium vine were observed under an Olympus light microscope.
- Table 1 shows the results of an antagonistic test of Verticillium vulgaris against Melon Fusarium wilt fungus at the in-vitro mouth. As can be seen from Table 1, the A-2, B-1, C-1 and MAFF235693 strains of the verti-serum 'Ryeki-' showed strong inhibition bands.
- FIGS. 4 to 6 A colony of the verteilium 're-force' was detected in the soil, root surface, and rhizosphere.
- the results of the number of cuffs (cfu) are shown in FIGS. 4 to 6, respectively.
- All strains were detected, indicating a good ability to form colonies (Figure 4).
- ATCC22612 strain, A-2 strain and A-3 strain exceeded 5 ⁇ 108 cfuZg.
- the A-2 and B-1 strains had high colony forming ability and excellent colonization (Fig. 5).
- the MAFF 235701, ATCC58908, and ATCC22612 strains formed a larger scale than other strains and were superior in fixation (FIG. 6).
- DSI symptom severity index
- % is calculated from the number of dead leaves Z and the total number of leaves X 100.For each plant, a plant with a grade of 0 is healthy and a plant with a grade of 100 based on the evaluation of the appearance of the plant leaves. Gave a rating from 0 to 100, such as death.
- Antagonism of vertilyzium.reiki- in the mouth of tomato wilt fungus It was examined on the medium. Verticillium's strain -20 was tested by dual culture with tomato wilt fungus. An agar disk of tomato wilt fungus was placed on one side of a potato dextrose agar (PDA: Sigma) plate, and on the other side of the same plate, a cultivar on a potato sucrose agar (PSA) was cultured. A disk was placed. After culturing at 25 ⁇ 0.1 ° C. for 3 weeks, the inhibition zone width (mm) was measured. The same experiment was performed three times. Hyphal interactions between the bacterium and the tomato wilt fungus were observed under an Olympus light microscope.
- PDA potato dextrose agar
- Table 2 shows the results of an antagonistic test at the in-vitro mouth of verteilyzil 'le force- against tomato wilt fungus. Suppression was clearly identified by the restriction of pathogen growth in the inhibition zone surrounding the colony of the vertical series.
- the strains of verteidium sclerosis excluding ATCC22611, ATCC58908, MAFF235696, MAFF235699, and MAFF235701 have antifungal activity against tomato wilt (V. dahliae). showed that.
- the Batarec strain, B-1, B2 strain, MAFF235690 strain and ATCC58907 strain significantly suppressed the growth of tomato wilt fungus.
- the stirred roots and attached soil were serially diluted with sterile distilled water, and 1 ml of the diluted suspension was added dropwise to rose bengal agar medium containing streptomycin sulfate and incubated at 25 ° C for 3 days. After the incubation, the colonies of the vertical series were counted. Each experiment was performed three times.
- a colony of the verteilium 're-force' was detected in soil, root surface, and rhizosphere. Koguchi- The results for one (cfu) number are shown in 9 to: LI diagrams, respectively. In the soil, all strains were detected and showed high colony forming ability (Fig. 9). Among them, ATCC22612 strain, A-1 strain, A-3 strain, C-1 strain, and ATCC589095 strain showed high colony forming ability. Batarec strain, ATCC58908 strain, and MAFF235701 strain did not show high colony forming ability.
- B-2, A-3, ATCC22612, ATCC58909, MAFF235696, MAFF235699, and MAFF235701 have a high colony forming ability of 1.83 X 104 to 2.29 X 104 spores Zml. And excellent fixability (Fig. 10).
- Seedlings which were not treated (pathogen) or not treated with tomato half-wilt fungus were prepared as a control. At the end of the experiment, stems and leaves were collected, and fresh and dry weights were measured. For each treatment, three replicas were made, each replica consisting of four plants. Using many withered stems, the symptom of the stem was evaluated by appearance symptom index and internal symptom degree. Because the controls in this experiment did not grow compared to the other treatments, 1 ml of the V. dahliae suspension was injected 2 weeks after seeding, and No. ivonex (lmlZ pot) 8 weeks after seeding. The above-mentioned appearance symptom index was obtained by the number of withered stems Z and the total number of stems X 100, and the internal symptom degree was graded from 0 to 3 based on the degree of discoloration of the plant.
- the Verti-Silium 'Ren-C-1 strain was the most effective in controlling the wilt of tomato half-wilt.
- the B-1 strain and the MAFF235690 strain were effective against the early stage of tomato wilt.
- the B-1 strain showed an increase in fresh weight (gZ plants) and dry weight (gZ plants) of tomato seedlings as compared to the pathogen control (FIGS. 14, 15).
- the result that the severity of the internal symptom was reduced by the strain of Verti-Silium® was almost equal to the appearance rate of the symptom in appearance. All strains showed a marked reduction in internal symptom counts as compared to the pathogen control.
- the -2 and J-1 strains showed a decrease in the severity of internal symptoms, as did the controls.
- Verticillium lecanii B-2 (Verticillium lecanii B-2) was used as the test microorganism.
- control plants were sprayed with spores (104 conidia Zml) of the powdery mildew fungus on the leaves and inoculated, and 2 to 4 weeks after inoculation, The transition was observed.
- the plants treated with the spores of the Verteilium 'Ren-B-2' strain and the plants in the control group were not inoculated with the spores of the powdery mildew fungus (uninoculated leaves), and 2 to 4 weeks later. Changes in the symptoms were observed (observation of powdery mildew caused by airborne disease).
- FIG. 16 shows the results of the above inoculation experiments.
- FIG. Fig. 16 shows the change of symptom 2 to 4 weeks after inoculation of the cucumber powdery mildew fungus as a symptom index in a graph.
- FIG. 17 shows photographs showing the disease status of the third leaf after 3 weeks in the control plot and the Verti-Silium 'Reiki-B-2 strain (50-ml treatment plot).
- the microbial pesticide of the present invention containing a verti-silica-reactive power inhibits the growth of above-ground plant pathogenic bacteria by being applied to plants, and also contains the verti-silica-reactive power of the present invention.
- a microbial pesticide for controlling soil diseases near the roots of plants it is possible to suppress the occurrence of soil diseases such as Fusarium disease and Verticillium disease, and to control diseases in the environment where plants grow.
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Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
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EP05737162A EP1774854A1 (en) | 2004-04-28 | 2005-04-27 | Microbial pesticide inhibiting the outbreak of plant disease damage |
JP2006512799A JPWO2005104853A1 (ja) | 2004-04-28 | 2005-04-27 | 植物病害の発生を抑制する微生物農薬 |
US11/568,369 US20080019947A1 (en) | 2004-04-28 | 2005-04-27 | Microbial Pesticide Inhibiting the Outbreak of Plant Disease Damage |
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JP2004133449 | 2004-04-28 | ||
JP2004-133449 | 2004-04-28 |
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WO2005104853A1 true WO2005104853A1 (ja) | 2005-11-10 |
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PCT/JP2005/007981 WO2005104853A1 (ja) | 2004-04-28 | 2005-04-27 | 植物病害の発生を抑制する微生物農薬 |
Country Status (6)
Country | Link |
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US (1) | US20080019947A1 (ja) |
EP (1) | EP1774854A1 (ja) |
JP (1) | JPWO2005104853A1 (ja) |
KR (1) | KR20070004972A (ja) |
CN (1) | CN1946298A (ja) |
WO (1) | WO2005104853A1 (ja) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006169115A (ja) * | 2004-12-13 | 2006-06-29 | Kumiai Chem Ind Co Ltd | バーティシリウム・レカニyk−920菌株およびそれを用いた害虫および植物病害の防除剤 |
JP2010070538A (ja) * | 2008-08-20 | 2010-04-02 | Miyagi Prefecture | 植物病害防除剤 |
CN102010825A (zh) * | 2011-01-20 | 2011-04-13 | 上海创博生态工程有限公司 | 一种防治黄瓜枯萎病的微生物复合菌剂及其制备方法 |
EP1942741B1 (de) * | 2005-11-04 | 2015-06-03 | Leibniz-Zentrum für Agrarlandschaftsforschung (ZALF) e.V. | Zusammensetzung und verfahren zur verhinderung von durch verticillium verursachten pflanzenschäden |
CN113151043A (zh) * | 2021-02-04 | 2021-07-23 | 塔里木大学 | 一株抗棉花黄萎病的根瘤菌dg3-1及其用途 |
CN114041383A (zh) * | 2021-09-30 | 2022-02-15 | 泰迪伦(武汉)节能环保科技有限公司 | 羟基离子水及其在生态种植中的应用 |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101248799B (zh) * | 2008-03-04 | 2011-10-19 | 云南农业大学 | 一种蜡蚧轮枝菌杀虫剂及其应用 |
CN102150689B (zh) * | 2010-12-15 | 2013-07-24 | 沈阳科丰牧业科技有限公司 | 一种蔬菜用复合微生物消毒剂 |
JP6351928B2 (ja) * | 2013-03-04 | 2018-07-04 | 片倉コープアグリ株式会社 | 農地及び農地に使用する資材の病原抑止力の評価方法並びに同方法を用いた農作物栽培システム |
CN109792953B (zh) * | 2019-01-29 | 2022-03-22 | 南京农业大学 | 一种菊花组培方法 |
CN112544311A (zh) * | 2020-12-01 | 2021-03-26 | 安徽金敦福农业科技有限公司 | 一种新型农作物土传病害传播阻断方法 |
Citations (2)
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---|---|---|---|---|
WO2003000050A2 (en) * | 2001-06-21 | 2003-01-03 | The Banks Group, Llc | Control of powdery mildew disease using verticillium lecanii |
JP2003335612A (ja) * | 2002-05-17 | 2003-11-25 | Japan Science & Technology Corp | 植物体表面定着性微生物農薬 |
-
2005
- 2005-04-27 KR KR1020067023925A patent/KR20070004972A/ko not_active Application Discontinuation
- 2005-04-27 JP JP2006512799A patent/JPWO2005104853A1/ja active Pending
- 2005-04-27 US US11/568,369 patent/US20080019947A1/en not_active Abandoned
- 2005-04-27 CN CNA2005800133498A patent/CN1946298A/zh active Pending
- 2005-04-27 WO PCT/JP2005/007981 patent/WO2005104853A1/ja active Application Filing
- 2005-04-27 EP EP05737162A patent/EP1774854A1/en not_active Withdrawn
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003000050A2 (en) * | 2001-06-21 | 2003-01-03 | The Banks Group, Llc | Control of powdery mildew disease using verticillium lecanii |
JP2003335612A (ja) * | 2002-05-17 | 2003-11-25 | Japan Science & Technology Corp | 植物体表面定着性微生物農薬 |
Non-Patent Citations (1)
Title |
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VERHAAR M.A. ET AL: "Glasshouse Experiments on Biocontrol of Cucumber Powdery Mildew(sphaerotheca fuliginea)by the Mycoparasites Verticillium lecanii and Sporothrix rugulosa.", BIOLOGICAL CONTROL., vol. 6, no. 3, 1996, pages 353 - 360, XP002990136 * |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006169115A (ja) * | 2004-12-13 | 2006-06-29 | Kumiai Chem Ind Co Ltd | バーティシリウム・レカニyk−920菌株およびそれを用いた害虫および植物病害の防除剤 |
JP4644478B2 (ja) * | 2004-12-13 | 2011-03-02 | クミアイ化学工業株式会社 | バーティシリウム・レカニyk−920菌株およびそれを用いた害虫および植物病害の防除剤 |
EP1942741B1 (de) * | 2005-11-04 | 2015-06-03 | Leibniz-Zentrum für Agrarlandschaftsforschung (ZALF) e.V. | Zusammensetzung und verfahren zur verhinderung von durch verticillium verursachten pflanzenschäden |
JP2010070538A (ja) * | 2008-08-20 | 2010-04-02 | Miyagi Prefecture | 植物病害防除剤 |
CN102010825A (zh) * | 2011-01-20 | 2011-04-13 | 上海创博生态工程有限公司 | 一种防治黄瓜枯萎病的微生物复合菌剂及其制备方法 |
CN102010825B (zh) * | 2011-01-20 | 2013-05-29 | 上海创博生态工程有限公司 | 一种防治黄瓜枯萎病的微生物复合菌剂及其制备方法 |
CN113151043A (zh) * | 2021-02-04 | 2021-07-23 | 塔里木大学 | 一株抗棉花黄萎病的根瘤菌dg3-1及其用途 |
CN114041383A (zh) * | 2021-09-30 | 2022-02-15 | 泰迪伦(武汉)节能环保科技有限公司 | 羟基离子水及其在生态种植中的应用 |
CN114041383B (zh) * | 2021-09-30 | 2023-06-23 | 泰迪伦(武汉)节能环保科技有限公司 | 一种生态种植技术 |
Also Published As
Publication number | Publication date |
---|---|
CN1946298A (zh) | 2007-04-11 |
JPWO2005104853A1 (ja) | 2008-03-13 |
KR20070004972A (ko) | 2007-01-09 |
EP1774854A1 (en) | 2007-04-18 |
US20080019947A1 (en) | 2008-01-24 |
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