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  • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
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  • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
  • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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  • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
  • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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  • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
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Definitions

• the present invention relates to a series of 5- and 6-membered nitrogen-containing heterocycles, their synthesis and their use, for example, for the treatment of disorders and conditions mediated by the histamine H 3 receptor.
• Histamine ⁇ 2-(imidazol-4-yl)ethylamine ⁇ is a transmitter substance. Histamine exerts a physiological effect via multiple distinct G-protein coupled receptors. It plays a role in immediate hypersensitivity reactions and is released from mast cells following antigen IgE antibody interaction. The actions of released histamine on the vasculature and smooth muscle system account for the symptoms of the allergic response. These actions occur at the Hi receptor (Ash, A.S.F. and Schild, H.O., Br. J. Pharmac. Chemother. 1966, 27:427-439) and are blocked by the classical antihistamines (e.g. diphenhydramine).
• Histamine is also an important regulator of gastric acid secretion through its action on parietal cells. These effects of histamine are mediated via the H receptor (Black, J.W. et al., Nature 1972, 236:385-390) and are blocked by H 2 receptor antagonists (e.g. cimetidine).
• H 2 receptor antagonists e.g. cimetidine
• the third histamine receptor — H 3 — was first described as a presynaptic autoreceptor in the central nervous system (CNS) (Arrang, J.-M. et al., Nature 1983, 302:832- 837) controlling the synthesis and release of histamine.
• H 3 receptors are also located presynaptically as heteroreceptors on serotonergic, noradrenergic, dopaminergic, cholinergic, and GABAergic (gamma-aminobutyric acid containing) neurons. These H 3 receptors have also recently been identified in peripheral tissues such as vascular smooth muscle. Consequently, there are many potential therapeutic applications for histamine H 3 agonists, antagonists, and inverse agonists. (See: "The Histamine H 3 Receptor-A Target for New Drugs", Leurs, R., and Timmerman, H., (Eds.), Elsevier, 1998; Morisset, S.
• a fourth histamine receptor — H — was recently described by Oda, T. et al. (J. Biol. Chem. 2000, 275(47):36781 -36786).
• the potential use of histamine H 3 agonists in sleep/wake and arousal/vigilance disorders is suggested based on animal studies (Lin, J.-S. et al., Brain Res. 1990, 523:325-330; Monti, J.M. et al., Eur. J. Pharmacol. 1991 , 205:283-287). Their use in the treatment of migraine has also been suggested (McLeod, R.L. et al., Soc.
• Neurosci. Abstr. 1996, 22:2010 based on their ability to inhibit neurogenic inflammation.
• Other applications could include a protective role in myocardial ischemia and hypertension where blockade of norepinephrine release is beneficial (Imamura, M. et al., J. Pharmacol. Exp. Ther. 1994, 271 (3): 1259-1266).
• histamine H 3 agonists may be beneficial in asthma due to their ability to reduce non- adrenergic non-cholinergic (NANC) neurotransmission in airways and to reduce microvascular leakage (lchinose, M. and Barnes, P.J., Eur. J. Pharmacol. 1989, 174:49-55).
• NANC non- adrenergic non-cholinergic
• histamine H 3 antagonists and inverse agonists have similarly been proposed based on animal pharmacology experiments with known histamine H 3 antagonists (e.g. thioperamide). These include dementia, Alzheimer's disease (Panula, P. et al., Soc. Neurosci. Abstr. 1995, 21 :1977), epilepsy (Yokoyama, H. et al., Eur. J. Pharmacol.
• narcolepsy with or without associated cataplexy, cataplexy, disorders of sleep/wake homeostasis, idiopathic somnolence, excessive daytime sleepiness (EDS), circadian rhythm disorders, sleep/fatigue disorders, fatigue, drowsiness associated with sleep apnea, sleep impairment due to perimenopausal hormonal shifts, jet lag, Parkinson's-related fatigue, multiple sclerosis (MS)- related fatigue, depression-related fatigue, chemotherapy-induced fatigue, eating disorders (Machidori, H. et al., Brain Res. 1992, 590:180-186), motion sickness, vertigo, attention deficit hyperactivity disorders (ADHD), learning and memory (Barnes, J.C.
• Histamine H 3 antagonists alone or in combination with a histamine H-i antagonist, are reported to be useful for the treatment of upper airway allergic response (U.S. Patent Nos.
• imidazole-containing compounds are substrates for histamine methyl transferase, the major histamine metabolizing enzyme in humans, which leads to shortened half-lives and lower bioavailability (see Rouleau, A. et al., J. Pharmacol. Exp. Ther. 1997, 281 (3):1085-1094).
• imidazole-containing drugs via their interaction with the cytochrome P 45 o monooxygenase system, can participate in unfavorable biotransformations due to enzyme induction or enzyme inhibition (see: Kapetanovic, I.M. and Kupferberg, H.J., Drug Metab. Dispos. 1984, 12(5):560-564; Sheets, J.J.
• Patent 5,352,707 PCT Application WO 99/42458, Aug. 26, 1999; PCT Application WO 02/076925; and European Patent Application 0978512, Feb. 9, 2000.
• a more recent review of this topic was presented (Tozer, M.T. and Kalindjian, S.B. Exp. Opin. Ther. Patents 2000, 10:1045). Additional publications and patents, concerning both histamine H 3 agonists and antagonists, have appeared since the publication of the Leurs monograph.
• the compounds of the present invention do not contain the imidazole moiety, and its inherent liabilities, and yet maintain potency at the human H 3 receptor as determined by receptor binding to the human histamine H 3 receptor (see Lovenberg, T.W. et al., Mol. Pharmacol. 1999, 55:1101-1107). Screening using the human receptor is particularly important for the identification of new therapies for the treatment of human disease. Conventional binding assays, for example, are determined using rat synaptosomes (Garbarg, M. et al., J. Pharmacol. Exp. Ther.
• A, B and B 2 are CH; II) A is CH, one of B 1 and B 2 is N, and the other of B 1 and B 2 is CH; or III) A is absent, B 1 is CH, and B 2 is O; L is -C ⁇ . 4 alkylene- or a covalent bond; Q is -(CH 2 ) m O- -(CH 2 ) n C ⁇ C- (where the -O- and -C ⁇ C- portions are directly attached to the ring), carbonyl, or thiocarbonyl; m is 2, 3, or 4; n is 1 , 2, 3, or 4;
• R q is independently selected from the group consisting of -d. 6 alkyl, halo, -OH, -Od-ealkyl, -CN, -N0 2 , -CF 3 , and -COOC 1-4 alkyl, R 3 , optionally mono- or di-substituted with R ⁇ , is independently selected from the group consisting of -H, -C ⁇ .
• R* is independently selected from the group consisting of is independently selected from the group consisting of -Ci- ⁇ alkyl, halo, -OH, -OC ⁇ - 6 alkyl. -CN, -N0 2 , -CF 3 , and -COOC ⁇ . alkyl; and enantiomers, diastereomers, hydrates, solvates and pharmaceutically acceptable salts, esters and amides thereof.
• isomeric forms of the compounds of formula (I), and of their pharmaceutically acceptable salts, esters, and amides are encompassed within the present invention, and reference herein to one of such isomeric forms is meant to refer to at least one of such isomeric forms.
• compounds according to this invention may exist, for example in a single isomeric form whereas other compounds may exist in the form of a regioisomeric mixture.
• the invention also features pharmaceutical compositions containing such compounds and methods of using such compositions in the treatment or prevention of disease states mediated by histamine H 3 receptor activity.
• the invention also features a pharmaceutical composition comprising a compound of the invention and a pharmaceutically acceptable carrier; and methods of preparing or formulating such compositions.
• a composition of the invention may further include more than one compound of the invention, or a combination therapy (combination formulation or combination of differently formulated active agents).
• the invention also provides methods of treating certain conditions and diseases, each of which methods includes administering a therapeutically effective (or jointly effective) amount of a compound or composition of the invention to a subject in need of such treatment.
• the disclosed compounds are useful in methods for treating or preventing neurologic disorders including sleep/wake and arousal/vigilance disorders (e.g.
• insomnia and jet lag attention deficit hyperactivity disorders
• ADHD attention deficit hyperactivity disorders
• learning and memory disorders cognitive dysfunction, migraine, neurogenic inflammation, dementia, mild cognitive impairment (pre-dementia), Alzheimer's disease, epilepsy, narcolepsy with or without associated cataplexy, cataplexy, disorders of sleep/wake homeostasis, idiopathic somnolence, excessive daytime sleepiness (EDS), circadian rhythm disorders, sleep/fatigue disorders, fatigue, drowsiness associated with sleep apnea, sleep impairment due to perimenopausal hormonal shifts, Parkinson's-related fatigue, MS-related fatigue, depression- related fatigue, chemotherapy-induced fatigue, eating disorders, obesity, motion sickness, vertigo, schizophrenia, substance abuse, bipolar disorders, manic disorders and depression, as well as other histamine H 3 receptor mediated disorders such as upper airway allergic response, asthma, itch, nasal congestion and allergic rhinitis in a subject in need thereof.
• ADHD attention deficit hyperactivity disorders
• the invention features methods for preventing, inhibiting the progression of, or treating upper airway allergic response, asthma, itch, nasal congestion and allergic rhinitis.
• the disclosed compounds may be used in a combination therapy method including administering a jointly effective dose of an H 3 antagonist and administering a jointly effective dose of a histamine H-i antagonist, such as loratidine (CLARITINTM), desloratidine (CLARINEXTM), fexofenadine (ALLEGRATM) and cetirizine (ZYRTECTM), for the treatment of allergic rhinitis, nasal congestion, and allergic congestion.
• a histamine H-i antagonist such as loratidine (CLARITINTM), desloratidine (CLARINEXTM), fexofenadine (ALLEGRATM) and cetirizine (ZYRTECTM
• the disclosed compounds may be used in a combination therapy method, including administering a jointly effective dose of an H 3 antagonist and administering a jointly effective dose of a neurotransmitter re-uptake blocker, such as a selective serotonin re-uptake inhibitor (SSRI), a serotonin-norepinephrine reuptake inhibitor, a noradrenergic reuptake inhibitor, or a non-selective serotonin, dopamine or norepinephrine reuptake inhibitor, including fluoxetine (PROZACTM), sertraline (ZOLOFTTM), paroxetine (PAXILTM) and amitryptyline, for the treatment of depression, mood disorders or schizophrenia.
• SSRI selective serotonin re-uptake inhibitor
• ZOLOFTTM sertraline
• PAXILTM paroxetine
• amitryptyline for the treatment of depression, mood disorders or schizophrenia.
• the disclosed compounds may be used in a combination therapy method, including administering a jointly effective dose of an H 3 antagonist and administering a jointly effective dose of modafinil, for example, for the treatment of narcolepsy, excessive daytime sleepiness (EDS), Alzheimer's disease, depression, attention deficit disorders, MS-related fatigue, post-anesthesia grogginess, cognitive impairment, schizophrenia, spasticity associated with cerebral palsy, age-related memory decline, idiopathic somnolence, or jet-lag.
• EDS excessive daytime sleepiness
• Alzheimer's disease depression
• attention deficit disorders MS-related fatigue
• post-anesthesia grogginess cognitive impairment
• schizophrenia spasticity associated with cerebral palsy
• age-related memory decline idiopathic somnolence
• jet-lag idiopathic somnolence
• the A- and B-containing ring is selected from the group consisting of pyridine, pyrazine, and isoxazole.
• A, B 1 and B 2 are CH; B 1 is N and B 2 and A are CH; or A is absent, B 1 is CH, and B 2 is O.
• the A- and B-containing ring is pyridine.
• the A- and B-containing ring is a 3,6-disubstituted pyridine.
• the A- and B-containing ring is a 2,5-disubstituted pyridine.
• the A- and B-containing ring is a 2,5-disubstituted pyrazine.
• the A- and B-containing ring is a 3,5-disubstituted isoxazole. Even more preferably, A is CH or A is absent. Even more preferably, B 1 is CH or N. Even more preferably, B 2 is CH or O. Preferably, L is methylene.
• Q is selected from the group consisting of propylenoxy, ethylenoxy, propyn-1 -ylene, butyn-1 -ylene, carbonyl, and thiocarbonyl.
• Q is propylenoxy, butyn-1 -ylene, or carbonyl.
• Q is carbonyl.
• R 1 is independently selected from the group consisting of -H, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, methoxyethyl, hydroxyethyl, piperidinylethyl, morpholinylethyl, pyridylethyl, diethylaminoethyl, propenyl, propargyl, cyclopropyl, cyclopentyl, cyclohexyl, phenyl, benzyl, pyridinyl, pyrrolyl, pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, and azepanyl.
• R 1 is independently selected from the group consisting of -H, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, methoxyethyl, cyclopropyl, piperidinylethyl, morpholinylethyl, pyridylethyl, and diethylaminoethyl. Even more preferably, R 1 is independently selected from the group consisting of -H, methyl, and methoxyethyl.
• R 2 is independently selected from the group consisting of methyl, ethyl, propyl, isopropyl, butyl, isobutyl, methoxyethyl, hydroxyethyl, piperidinylethyl, morpholinylethyl, pyridylethyl, diethylaminoethyl, propenyl, propargyl, cyclopropyl, cyclopentyl, cyclohexyl, phenyl, benzyl, pyridinyl, pyrrolyl, pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, and azepanyl.
• R 2 is independently selected from the group consisting of methyl, ethyl, propyl, isopropyl, butyl, isobutyl, methoxyethyl, cyclopropyl, piperidinylethyl, morpholinylethyl, pyridylethyl, and diethylaminoethyl. Even more preferably, R 2 is independently selected from the group consisting of methyl and methoxyethyl.
• said ring is selected from the group consisting of piperidine, morpholine, thiomorpholine, piperazine, and pyrrolidine.
• R 1 and R 2 may be taken together with the nitrogen of attachment to form a ring selected from the group consisting of piperidine, morpholine, and piperazine.
• R and R 2 may be taken together with the nitrogen of attachment to form 4-fluoropiperidine.
• R 1 and R 2 may be taken together with the nitrogen of attachment to form a ring selected from the group consisting of piperidine and morpholine.
• R 3 is independently selected from the group consisting of -H, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, methoxyethyl, hydroxyethyl, piperidinylethyl, morpholinylethyl, pyridylethyl, diethylaminoethyl, propenyl, propargyl, cyclopropyl, cyclopentyl, cyclohexyl, phenyl, benzyl, pyridinyl, pyrrolyl, pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, and azepanyl.
• R 3 is independently selected from the group consisting of -H, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, methoxyethyl, cyclopropyl, piperidinylethyl, morpholinylethyl, pyridylethyl, and diethylaminoethyl. Even more preferably, R 3 is independently selected from the group consisting of -H, methyl, and methoxyethyl.
• R 4 is independently selected from the group consisting of methyl, ethyl, propyl, isopropyl, butyl, isobutyl, methoxyethyl, hydroxyethyl, piperidinylethyl, morpholinylethyl, pyridylethyl, diethylaminoethyl, propenyl, propargyl, cyclopropyl, cyclopentyl, cyclohexyl, phenyl, benzyl, pyridinyl, pyrrolyl, pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, and azepanyl.
• R 4 is independently selected from the group consisting of methyl, ethyl, propyl, isopropyl, butyl, isobutyl, methoxyethyl, cyclopropyl, piperidinylethyl, morpholinylethyl, pyridylethyl, and diethylaminoethyl. Even more preferably, R 4 is independently selected from the group consisting of methyl and methoxyethyl. Preferably, where R 3 and R 4 are taken together with the nitrogen of attachment to form a ring, said ring is selected from the group consisting of piperidine, morpholine, thiomorpholine, piperazine, and pyrrolidine.
• R 3 and R 4 may be taken together with the nitrogen of attachment to form a ring selected from the group consisting of piperidine, morpholine, and piperazine.
• R 3 and R 4 may be taken together with the nitrogen of attachment to form 4-fluoropiperidine.
• R 3 and R 4 may be taken together with the nitrogen of attachment to form a ring selected from the group consisting of piperidine and piperazine.
• any of the preferred substituents described above that can be optionally further substituted with any of R p , R q , R ⁇ , or R 1 according to formula (I) are intended to be so optionally substituted. It is understood that some compounds referred to herein are chiral and/or have geometric isomeric centers, for example E- and Z- isomers. The present invention encompasses all such optical isomers, including stereoisomers and racemic mixtures, diastereomers, and geometric isomers that possess the activity that characterizes the compounds of this invention. Compounds of the invention may exist as single enantiomers, mixtures of enantiomers, or racemic mixtures. In certain embodiments, the absolute configuration of a single enantiomer may be unknown.
• certain compounds referred to herein can exist in solvated as well as unsolvated forms. It is understood that this invention encompasses all such solvated and unsolvated forms that possess the activity that characterizes the compounds of this invention.
• Compounds according to the present invention that have been modified to be detectable by some analytic technique are also within the scope of this invention.
• the compounds of the present invention may be labeled with radioactive elements such as 125 l, 18 F, 11 C, 64 Cu, and the like for use in imaging or for radioactive treatment of patients.
• an isotopically labeled compound such as an 18 F isotopically labeled compound that may be used as a probe in detection and/or imaging techniques, such as positron emission tomography (PET) and single-photon emission computed tomography (SPECT).
• PET positron emission tomography
• SPECT single-photon emission computed tomography
• compounds of the present invention labeled with 18 F or 11 C may be used as a positron emission tomography (PET) molecular probe for studying disorders mediated by the histamine H 3 receptor and the serotonin transporter.
• PET positron emission tomography
• Another example of such compounds is an isotopically labeled compound, such as a deuterium and/or tritium labeled compound that may be used in reaction kinetic studies.
• the compounds described herein may be reacted with an appropriate functionalized radioactive reagents using conventional chemistry to provide radiolabeled compounds.
• Pharmaceutically acceptable salts, esters, and amides include carboxylate salts (e.g., C ⁇ . 8 alkyl, C 3 - 8 cycloalkyl, aryl, C 2 - ⁇ oheteroaryl, or C 2 - 10 non-aromatic heterocyclic), amino addition salts, acid addition salts, esters, and amides that are within a reasonable benefit/risk ratio, pharmacologically effective and suitable for contact with the tissues of patients without undue toxicity, irritation, or allergic response.
• carboxylate salts e.g., C ⁇ . 8 alkyl, C 3 - 8 cycloalkyl, aryl, C 2 - ⁇ oheteroaryl, or C 2 - 10 non-aromatic heterocyclic
• amino addition salts e.g., amino addition salts, acid addition salts, esters
• Representative salts for compounds of formula (I) displaying basic functionality include hydrobromide, hydrochloride, sulfate, bisulfate, nitrate, acetate, oxalate, valerate, oleate, palmitate, stearate, laurate, borate, benzoate, lactate, phosphate, tosylate, citrate, maleate, fumarate, succinate, tartrate, naphthylate, mesylate, glucoheptonate, lactiobionate, and laurylsulfonate.
• Representative addition salts for compounds of formula (I) displaying acidic functionality are those that form non-toxic base salts with such compounds.
• These salts may include alkali metal and alkali earth cations such as sodium, potassium, calcium, and magnesium, as well as non-toxic ammonium, quaternary ammonium, and amine cations such as tetramethyl ammonium, methylammonium, trimethylammonium, and ethylammonium.
• alkali metal and alkali earth cations such as sodium, potassium, calcium, and magnesium
• non-toxic ammonium, quaternary ammonium, and amine cations such as tetramethyl ammonium, methylammonium, trimethylammonium, and ethylammonium.
• the term "administering” shall encompass the treatment of the various disorders described with the compound specifically disclosed or with a compound that may not be specifically disclosed, but which converts to the specified compound in vivo after administration to the patient.
• Conventional procedures for the selection and preparation of suitable prodrug derivatives are described, for example, in "Design of Prodrugs", ed. H. Bundgaard, Elsevier, 1985.
• the invention provides the esters, amides, and other protected or derivatized forms of the described compounds.
• Representative pharmaceutically acceptable amides of the invention include those derived from ammonia, primary C- ⁇ - 6 alkyl amines and secondary di(C ⁇ - 6 alkyl) amines.
• Secondary amines include 5- or 6-membered heterocyclic or heteroaromatic ring moieties containing at least one nitrogen atom and optionally between 1 and 2 additional heteroatoms.
• Preferred amides are derived from ammonia, C ⁇ alkyl primary amines, and di(C ⁇ - 2 alkyl)amines.
• Representative pharmaceutically acceptable esters of the invention include C ⁇ - 7 alkyl, C 5 - 7 Cycloalkyl, phenyl, and phenyl(C ⁇ . 6 )alkyl esters.
• Preferred esters include methyl esters.
• the present invention includes within its scope prodrugs of the compounds of this invention. In general, such prodrugs will be functional derivatives of the compounds that are readily convertible in vivo into the required compound.
• the term "administering” shall encompass the treatment of the various disorders described with the compound specifically disclosed or with a compound that may not be specifically disclosed, but which converts to the specified compound in vivo after administration to the patient.
• Conventional procedures for the selection and preparation of suitable prodrug derivatives are described, for example, in “Design of Prodrugs", ed. H. Bundgaard, Elsevier, 1985.
• the invention provides the esters, amides, and other protected or derivatized forms of the described compounds.
• Preferred compounds of the present invention are selected from the group consisting of:
• compounds of the present invention are selected from the group consisting of:
• compounds of the present invention are selected from the group consisting of:
• the aldehyde functionality can then be reacted under conditions of reductive amination to provide compounds of formula (IV).
• the aldehyde can be treated with a suitable amine, with or without the addition of an activating agent such as a protic or Lewis acid, and with an appropriate reducing agent such as sodium triacetoxyborohydride.
• the aldehyde may alternatively be reduced to an alcohol, converted to a leaving group such as a chloride and displaced with an appropriate amine as shown below in Scheme G.
• the chloride could also be displaced with cyanide anion, and the resulting nitrite reduced to homologate the linker by one additional carbon.
• the aldehyde may be reacted using Horner-Emmons chemistry followed by hydrogenation of the double bond to introduce an alkyl chain containing an additional two carbons.
• the ester can be converted to a range of amides of formula (I) using a primary or secondary amine, in the presence of a Lewis acid activating agent such as MgBr 2 .
• the carboxamide may be converted to its corresponding thioamides of formula (I) by treatment with P 2 S5 or Lawesson's reagent.
• compounds of formula (I) may also be produced as shown in Scheme C, with the following notes and additions.
• Heterocyclic hydroxy acids (VIII) may be reacted under peptide coupling conditions with a primary or secondary amine as described above to form amides (IX).
• the amide functionality may be reduced to the corresponding amine (X) using an appropriate reducing agent such as borane-dimethylsulfide.
• the hydroxyl functionality may be alkylated with a suitable alkylating agent to install the hydrocarbon linker (XI), such as 1-bromo-3-chloropropane or 1 -bromo-4- chloropropane, in the presence of a suitable base such as K 2 CO 3 .
• Ag 2 C0 3 may be used.
• the tethered leaving group may be displaced with a primary or secondary amine, with or without the addition of catalytic Kl, and in the presence of a suitable base such as Na 2 C0 3 .
• compounds of formula (X) may be converted directly to compounds of formula (I) under Mitsunobu conditions with an amine-functionalized alcohol such as 3-piperidin-1 -ylpropanol, standard or polymer-supported triphenylphosphine, and di-t-butyl azodicarboxylate, in a solvent such as dichloromethane.
• Compounds of formula (I) may also be prepared as decribed in Scheme D, with the following notes and additions.
• Compounds of formula (XII) where X is bromide may be converted to bromo aldehydes (XIII) by treatment with an organolithium reagent such as n-BuLi, and quenching the lithium anion with DMF.
• the intermediate lithium species may be converted to corresponding Grignard reagent in situ via treatment with n-BuMgCI.
• Bromo aldehydes can be prepared from commercially available carboxylic acids using methods known to one skilled in the art.
• the aldehydes (XIII) can be transformed into amines (XIV) using reductive amination conditions as described above.
• reagents such as N-phenyltrifluoromethane- sulfonimide or triflic anhydride
• a tertiary amine base such as triethylamine.
• the triflates and bromides may be coupled, typically under conditions of palladium catalysis, with terminal alkynes suitably functionalized with an amine substituent, such as 1 -but-3-ynyl-piperidine (Turner, S.C. et al. Bioorg. Med. Chem. Lett.
• the free hydroxyl group in isoxazoles (XVII) may be alkylated under Mitsunobu conditions, using a suitably functionalized amino alcohol such as 3-piperidin-1 - yl-propan-1 -ol, polymer-supported triphenylphosphine, and di-t-butyl azodicarboxylate, in a solvent such as dichloromethane.
• a suitably functionalized amino alcohol such as 3-piperidin-1 - yl-propan-1 -ol, polymer-supported triphenylphosphine, and di-t-butyl azodicarboxylate
• the alcohol could also be converted to amine (XVIII) using alkylation and displacement steps described above.
• the ester functionality may be reduced to form alcohols of formula (XIX).
• the alcohol may be converted to the corresponding chloride with thionyl chloride, and subsequently displaced with a suitable primary or secondary amine to form compounds of formula (I).
• the alcohol may be oxidized to the corresponding aldehyde and transformed under conditions of reductive amination as described above.
• Compounds prepared according to the schemes described above may be obtained as single enantiomers, mixtures of enantiomers, or racemic mixtures. Where racemic ( 1 : 1 ) and non-racemic (not 1 : 1 ) mixtures of enantiomers are obtained, single enantiomers may be isolated using conventional separation methods known to one skilled in the art.
• Particularly useful separation methods may include chiral chromatography, recrystallization, resolution, diastereomeric salt formation, or derivatization into diastereomeric adducts followed by separation.
• the compounds of the present invention are modulators of the histamine H 3 receptor, and as such, the compounds are useful in the treatment of histamine H 3 -mediated disease states.
• Compounds of the present invention may be administered in pharmaceutical compositions to treat patients (humans and other mammals) with disorders mediated by the H 3 receptor.
• the disclosed compounds alone or in combination (with, for example, a histamine H-, receptor antagonist), are useful for treating or preventing neurologic disorders including sleep/wake and arousal/vigilance disorders (e.g.
• insomnia and jet lag attention deficit hyperactivity disorders
• ADHD attention deficit hyperactivity disorders
• learning and memory disorders cognitive dysfunction, migraine, neurogenic inflammation, dementia, mild cognitive impairment (pre-dementia), Alzheimer's disease, epilepsy, narcolepsy with or without associated cataplexy, cataplexy, disorders of sleep/wake homeostasis, idiopathic somnolence, excessive daytime sleepiness (EDS), circadian rhythm disorders, sleep/fatigue disorders, fatigue, drowsiness associated with sleep apnea, sleep impairment due to perimenopausal hormonal shifts, Parkinson's- related fatigue, MS-related fatigue, depression-related fatigue, chemotherapy- induced fatigue, eating disorders, obesity, motion sickness, vertigo, schizophrenia, substance abuse, bipolar disorders, manic disorders and depression, as well as other histamine H 3 receptor mediated disorders such as upper airway allergic response, asthma, itch, nasal congestion and allergic rhinitis in a subject in need thereof.
• ADHD attention deficit hyperactivity disorders
• Excessive daytime sleepiness may occur with or without associated sleep apnea, shift work, fibromyalgia, MS, and the like.
• the present invention also provides pharmaceutical compositions comprising one or more compounds of this invention in association with a pharmaceutically acceptable carrier and optionally additional pharmaceutical agents such as H-i antagonists, SSRIs, or modafinil.
• the pharmaceutical compositions can be prepared using conventional pharmaceutical excipients and compounding techniques known to those skilled in the art of preparing dosage forms. It is anticipated that the compounds of the invention can be administered by oral, parenteral, rectal, topical, or ocular routes, or by inhalation. Preparations may also be designed to give slow release of the active ingredient.
• the preparation may be in the form of tablets, capsules, sachets, vials, powders, granules, lozenges, powders for reconstitution, liquid preparations, or suppositories.
• compounds may be administered by intravenous infusion or topical administration, but more preferably by oral administration.
• the compounds of the invention can be provided in the form of tablets or capsules, or as a solution, emulsion, or suspension. Tablets for oral use may include the active ingredient mixed with pharmaceutically acceptable excipients such as inert diluents, disintegrating agents, binding agents, lubricating agents, sweetening agents, flavoring agents, coloring agents and preservatives agents.
• Suitable inert fillers include sodium and calcium carbonate, sodium and calcium phosphate, lactose, starch, sugar, glucose, methyl cellulose, magnesium stearate, mannitol, sorbitol, and the like; typical liquid oral excipients include ethanol, glycerol, water and the like.
• Starch, polyvinyl-pyrrolidone, sodium starch glycolate, microcrystalline cellulose, and alginic acid are suitable disintegrating agents. Binding agents may include starch and gelatin.
• the lubricating agent if present, will generally be magnesium stearate, stearic acid or talc.
• the tablets may be coated with a material such as glyceryl monostearate or glyceryl distearate to delay absorption in the gastrointestinal tract, or may be coated with an enteric coating.
• Capsules for oral use include hard gelatin capsules in which the active ingredient is mixed with a solid, semi-solid, or liquid diluent, and soft gelatin capsules wherein the active ingredient is mixed with water, an oil such as peanut oil or olive oil, liquid paraffin, a mixture of mono and di-glycerides of short chain fatty acids, polyethylene glycol 400, or propylene glycol.
• Liquids for oral administration may be suspensions, solutions, emulsions or syrups or may be presented as a dry product for reconstitution with water or other suitable vehicles before use.
• Compositions of such liquid may contain pharmaceutically-acceptable excipients such as suspending agents (for example, sorbitol, methyl cellulose, sodium alginate, gelatin, hydroxyethylcellulose, carboxymethylcellulose, aluminium stearate gel and the like); non-aqueous vehicles, which include oils (for example, almond oil or fractionated coconut oil), propylene glycol, ethyl alcohol or water; preservatives (for example, methyl or propyl p-hydroxybenzoate or sorbic acid); wetting agents such as lecithin; and, if needed, flavoring or coloring agents.
• suspending agents for example, sorbitol, methyl cellulose, sodium alginate, gelatin, hydroxyethylcellulose, carboxymethylcellulose, aluminium stearate gel and the like
• the compounds of this invention may also be administered by non-oral routes.
• the compositions may be formulated for rectal administration as a suppository.
• parenteral use including intravenous, intramuscular, intraperitoneal, or subcutaneous routes, the compounds of the invention will generally be provided in sterile aqueous solutions or suspensions, buffered to an appropriate pH and isotonicity or in parenterally acceptable oil.
• Suitable aqueous vehicles include Ringer's solution and isotonic sodium chloride.
• Such forms will be presented in unit dose form such as ampules or disposable injection devices, in multi-dose forms such as vials from which the appropriate dose may be withdrawn, or in a solid form or pre-concentrate that can be used to prepare an injectable formulation.
• Another mode of administration of the compounds of the invention may utilize a patch formulation to affect transdermal delivery.
• the compounds of this invention may also be administered by inhalation, via the nasal or oral routes using a spray formulation consisting of the compound of the invention and a suitable carrier.
• Effective doses of the compounds of the present invention may be ascertained by conventional methods.
• the specific dosage level required for any particular patient will depend on a number of factors, including severity of the condition being treated, the route of administration, and the weight of the patient. In general, however, it is anticipated that the daily dose (whether administered as a single dose or as divided doses) will be in the range 0.01 to 1000 mg per day, more usually from 1 to 500 mg per day, and most usually from 10 to 200 mg per day.
• a typical dose will be expected to be between 0.0001 mg/kg and 15 mg/kg, especially between 0.01 mg/kg and 7 mg/kg, and most especially between 0.15 mg/kg and 2.5 mg/kg.
• oral doses range from about 0.05 to 200 mg/kg, daily, taken in 1 to 4 separate doses.
• Some compounds of the invention may be orally dosed in the range of about 0.05 to about 50 mg/kg daily, others may -be dosed at 0.05 to about 20 mg/kg daily, while still others may be dosed at 0.1 to about 10 mg/kg daily.
• Infusion doses can range from about 1 to 1000 ⁇ g/kg/min of inhibitor, admixed with a pharmaceutical carrier over a period ranging from several minutes to several days.
• compounds of the present invention may be mixed with a pharmaceutical carrier at a concentration of about 0.1 % to about 10% of drug to vehicle.
• the disclosed compounds are useful in combination with other therapeutic agents, including H-, receptor antagonists, H receptor antagonists, and neurotransmitter modulators such as SSRIs, serotonin-norepinephrine reuptake inhibitors, noradrenergic reuptake inhibitors, non-selective serotonin re-uptake inhibitors (NSSRIs), or other neuroactive agents such as modafinil.
• compositions or the disclosed drug combinations are known in the art for determining effective doses for therapeutic and prophylactic purposes for the disclosed pharmaceutical compositions or the disclosed drug combinations, whether or not formulated in the same composition.
• joint effective amount means that amount of each active compound or pharmaceutical agent, alone or in combination, that elicits the biological or medicinal response in a tissue system, animal or human that is being sought by a researcher, veterinarian, medical doctor or other clinician, which includes alleviation of the symptoms of the disease or disorder being treated.
• the term "jointly effective amount” refers to that amount of each active compound or pharmaceutical agent, alone or in combination, that inhibits in a subject the onset or progression of a disorder as being sought by a researcher, veterinarian, medical doctor or other clinician, the delaying of which disorder is mediated, at least in part, by the modulation of one or more histamine receptors.
• the present invention provides combinations of two or more drugs wherein, for example, (a) each drug is administered in an independently therapeutically or prophylactically effective amount; (b) at least one drug in the combination is administered in an amount that is sub- therapeutic or sub-prophylactic if administered alone, but is therapeutic or prophylactic when administered in combination with the second or additional drugs according to the invention; or (c) both drugs are administered in an amount that is sub-therapeutic or sub-prophylactic if administered alone, but are therapeutic or prophylactic when administered together. Combinations of three or more drugs are analogously possible. Methods of combination therapy include co-administration of a single formulation containing all active agents; essentially contemporaneous administration of more than one formulation; and administration of two or more active agents separately formulated.
• Step A 6-Formyl-nicotinic acid methyl ester.
• Step B 6-Piperidin-1-ylmethyl-nicotinic acid methyl ester.
• 6- formyl-nicotinic acid methyl ester (0.200 g, 1.21 mmol) and piperidine (0.14 mL, 1.33 mmol) in DCM (15 mL) was added NaB(OAc) 3 H (0.380 g, 1.80 mmol).
• the reaction was diluted with 1 N NaOH (10 mL) and extracted with DCM (2x50mL). The organic layers were combined, dried (Na 2 S0 ), and concentrated.
• Step C (4-lsopropyl-piperazin-1 -yl)-(6-piperidin-1 -ylmethyl-pyridin-3-vD- methanone.
• Step A 6-(4-lsopropyl-piperazine-1-carbonyl)-nicotinic acid methyl ester.
• Step D (4-lsopropyl-piperazin-1 -yl)-(5-piperidin-1 -ylmethyl-pyridin-2-yl)- methanone.
• 6-(4-isopropyl-piperazine-1 -carbonyl)-pyridine-3- carbaldehyde (0.250 g, 0.96 mmol) and piperidine (0.11 mL, 1.10 mmol) in DCM (20 mL) was added NaB(OAc) 3 H (0.300 g, 1.44 mmol).
• 1 N NaOH (15 mL) was added and the mixture was extracted with DCM (3x25mL). The organic layers were combined, dried (Na2S0 ), and concentrated.
• Step B 6-Piperidin-1 -ylmethyl-pyridin-3-ol.
• THF 100 mL
• borane-dimethylsulfide complex 1.75 mL, 18.9 mmol
• Step C 5-(3-Chloro-propoxy)-2-piperidin-1 ylmethyl-pyridine.
• Step P 2-Piperdin-1 -ylmethyl-5-(3-piperdin-1 -yl-propoxy)-pyridine.
• a solution of 5-(3-chloro-propoxy)-2-piperidin-1 ylmethyl-pyridine (0.25 g, 0.86 mmol), piperidine (0.09 mL, 0.94 mmol), Kl (0.003 g, 0.017 mmol), and Na 2 C0 3 (0.045 g, 0.43 mmol) in 1 -butanol ⁇ 5 mL) was heated to 95 °C. After 18 h the reaction mixture was concentrated, diluted with DCM (50 mL) and filtered through a pad of diatomaceous earth.
• Step A (6-Bromo-pyridin-3-yl)-piperidin-1 -yl-methanone.
• the intermediate is prepared in a similar fashion as described in Example 4, Step A, substituting 6- bromo-3-pyridine carboxylic acid for 5-hydroxy-2-pyridine carboxylic acid.
• Step B 2-Bromo-5-piperidin-1 -ylmethyl-pyridine.
• the intermediate is prepared in a similar fashion as described in Example 4, Step B, substituting (6-bromo- pyridin-3-yl)-piperidin-1 -yl-methanone for (5-hydroxy-pyridin-2-yl)-piperidin-1 -yl- methanone.
• Step C 5-Piperidin-1 -ylmethyl-2-(3-piperidin-1 -yl-propoxy)-pyridine.
• 3-piperidin-1 -yl-propan-1-ol 1.1 mmol.
• 2-bromo-5-piperidin-1 -ylmethyl-pyridine (1 mmol) is added to the mixture.
• the reaction is extracted with ethyl acetate (100 mL) and washed with 1 N NaHC0 3 (50 mL) and H 2 0 (3x50 mL). The organic layer is dried, concentrated, and chromatographed on Si0 2 to provide the title compound.
• Step A Trifluoro-methanesulfonic acid 5-piperidin-1 -ylmethyl-pyridin-2-yl ester.
• Step B Trifluoro-methanesulfonic acid 5-piperidin-1 -ylmethyl-pyridin-2-yl ester.
• Step A (6-Chloro-pyridin-3-yl)-(4-isopropyl-piperazin-1 -yl)-methanone.
• 6-chloronicotinic acid (0.985 g, 6.25 mmol) and 1 -isopropyl- piperazine (1.50 g, 7.50 mmol) in DCM (100 mL) was added HOBt (1.20 g, 9.40 mmol), EDC (1.80 g, 9.40 mmol), and N-methyl morpholine (3.4 mL, 31.3 mmol).
• HOBt 1.20 g, 9.40 mmol
• EDC (1.80 g, 9.40 mmol
• N-methyl morpholine 3.4 mL, 31.3 mmol
• Step B (4-lsopropyl-piperazin-1 -ylH6-(2-piperidin-1 -yl-ethylamino)-pyridin-3- yll-methanone.
• Step A (2-Chloro-pyridin-4-yl)-(4-isopropyl-piperazin-1 -yl)-methanone.
• the title compound was prepared in a manner similar to that described in Step A of Example 9 using 2-chloro-isonicotinic acid and 1 -isopropyl-piperazine.
• Step A (2-Chloro-pyridin-3-yl)-(4-isopropyl-piperazin-1 -yl)-methanone.
• the title compound was prepared in a manner similar to that described in Step A of Example 9 using 2-chloro-nicotinic acid and 1 -isopropyl-piperazine.
• Step B (4-lsopropyl-piperazin-1 -ylM2-(2-piperidin-1 -yl-ethylamino)-pyridin-3- yli-methanone.
• Step A (5-Bromo-pyridin-3-yl)-methanol.
• 5-bromonicotinic acid (20.00 g, 97.00 mmol) in anhydrous THF (250 mL) was added a solution of BH 3 (1 M in THF, 197 mL) slowly at rt over a period of 1 h.
• the reaction mixture was stirred for 1 h at rt and then was heated at reflux temperature for 5 h.
• the reaction mixture was cooled to rt and treated with 1 M HCI (100 mL) drop-wise.
• the resulting mixture was stirred for 1 h and treated with 10% aq. NaOH until pH 10.
• the solution was then extracted with ethyl acetate (4x200 mL).
• Step C 3-Bromo-5-piperidin-1 -ylmethyl-pyridine.
• 5-Bromo-pyridine-3- carbaldehyde (0.190g, 1 mmol), NaB(OAc) 3 H (0.320 g, 1.50 mmol) and piperidine (0.090 g, 1.05 mmol) were suspended in DCM (8 mL) and the reaction mixture was stirred overnight at rt. The reaction was quenched by the addition of 1 M NaOH solution (5 mL) and the mixture was stirred for 1 h. The reaction mixture was extracted with DCM (3x15 mL).
• Step P 3-(4-Piperidin-1 -yl-but-1 -vnyl)-5-piperidin-1 -ylmethyl-pyridine.
• Step A 4-(5-Bromo-pyridin-3-ylmethyl)-morpholine.
• the title compound was prepared in a similar way as described in Example 18, Step C, using 5-bromo- pyridine-3-carbaldehyde and morpholine.
• the crude compound was purified (Si0 2 : 0-3% 2 M NH 3 in MeOH/DCM) to provide the title compound (61 %).
• Step B 4-[5-(4-Piperidin-1 -yl-but-1 -vnyl)-pyridin-3-ylmethvH-morpholine.
• Step A 6-Bromo-pyridine-2-carbaldehyde. To a -10 ⁇ C solution of n-BuLi (2.5
• the mixture was transferred via cannula to a -10 ⁇ C solution of DMF (1.9 g, 26 mmol) in toluene (10 mL).
• the solution was allowed to stand between -5 ⁇ C and -10 g C for 30 min and then was transferred into a solution of citric acid (8.00 g) in H 2 0 (15 mL), maintaining the temperature below 20 e C.
• the resulting solution was stirred for 10 min and the layers were separated.
• the organic layer was dried over Na 2 S0 4 , filtered, and concentrated.
• the residue was purified (Si0 2 : 10% ethyl acetate/hexanes) to afford the title compound (1.94 g, 52%).
• Step B 2-Bromo-6-piperidin-1 -ylmethyl-pyridine.
• the title compound was prepared in a manner similar to that described in Example 18, Step C, using 6- bromo-pyridine-2-carbaldehyde (0.406 g, 2.18 mmol) and piperidine (0.186 g, 2.18 mmol).
• the crude product was purified (Si0 2 : 0-4% 2 M NH 3 in MeOH/DCM) to provide the title compound (0.46 g, 83%).
• Step C 2-(4-Piperidin-1 -yl-but-1 -vnyl)-6-piperidin-1 -ylmethyl-pyridine.
• Step A 4-(6-Bromo-pyridin-2-ylmethyl)-morpholine.
• the title compound was prepared in a manner similar to that described in Example 18, Step C, using 6- bromo-pyridine-2-carbaldehyde (0.415 g, 2.23 mmol) and morpholine (0.195 g, 2.23 mmol).
• the crude product was purified (Si0 2 : 0-3% 2 M NH 3 in MeOH/DCM) to give the title compound (0.352 g, 61%).
• Step B 4-[6-(4-Piperidin-1 -yl-but-1 -vnyl)-pyridin-2-ylmethvn-morpholine.
• Step A (2-Methoxy-ethyl)-[6-(4-piperidin-1 -yl-but-1 -ynyl)-pyridin-2-ylmethyl]-amine.
• Step B (6-Bromo-pyridin-2-ylmethyl)-(2-methoxy-ethyl)-amine.
• the title compound was prepared in a manner similar to that described in Example 18, Step C, using 6-bromo-pyridine-2-carbaldehyde (0.275 g, 1.48 mmol) and 2- methoxy-ethylamine (0.111 g, 1.48 mmol).
• the crude product was purified (Si0 2 : 0-5% MeOH/DCM) to yield the title compound (0.34 g, 94%).
• Step A 4-lsopropyl-piperazin-1 -yl)-(5-piperidin-1 -ylmethyl-pyrazin-2-yl)-methanone.
• Step A 5-Dibromomethyl-pyrazine-2-carboxylic acid methyl ester.
• 5-Methyl- pyrazine-2-carboxylic acid methyl ester (1.60 g, 10.5 mmol; Macdonald, S.J.F. et al. J.Med.Chem.
• N-bromosuccinimide (5.62 g, 31.6 mmol)
• dibenzoyl peroxide (0.255 g, 1.05 mmol) were dissolved in CCI 4 (80 mL).
• the mixture was heated at reflux for 18 h.
• the reaction mixture was cooled and washed with 10% aq. Na 2 S0 3 (2x20 mL) and H 2 0 (1x30 mL).
• the organic phase was dried over Na 2 S0 4 , filtered, and concentrated to yield a brown oily crude material (2.41 g).
• the crude product was purified (Si0 2 : 0- 20% ethyl acetate/hexanes) to give the title compound (1.00 g, 31%).
• Step B 5-Formyl-pyrazine-2-carboxylic acid methyl ester.
• a solution of 5- dibromomethyl-pyrazine-2-carboxylic acid methyl ester (1.00 g, 3.23 mmol) in a mixture of ethanol (20 mL) and THF (10 mL) was heated to 80 9 C.
• a solution of silver nitrate (2.20 g, 12.9 mmol) in H 2 0 (4 mL) was added.
• the reaction mixture was heated at 80 e C for 1.25 h and was filtered while hot. The filtrate was concentrated to yield the title compound (1.36 g). This material was carried to the next step without purification.
• Step C 5-Piperidin-1 -ylmethyl-Pyrazine-2-carboxylic acid methyl ester.
• a mixture of 5-formyl-pyrazine-2-carboxylic acid methyl ester (0.400 g, 2.40 mmol), piperidine (0.204 g, 2.40 mmol), and NaB(OAc) 3 H (1.40 g, 3.60 mmol) in DCM (10 mL) was stirred for 18 h at rt.
• the reaction was quenched by the addition of 10% aq. NaOH (10 mL) and the mixture was stirred for 30 min.
• the mixture was extracted with DCM (3x20 mL).
• the combined organic extracts were dried over Na 2 S0 4 , filtered, and concentrated to yield the title compound (0.130 g, 23%).
• Step P 5-Piperidin-1 -ylmethyl-pyrazine-2-carboxylic acid.
• a mixture of 5- piperidin-1 -ylmethyl-pyrazine-2-carboxylic acid methyl ester (0.125 g, 0.53 mmol) in dioxane (3 mL) was treated with 1 M aq. LiOH (0.53 mL, 0.53 mmol) and stirred for 18 h. The mixture was concentrated to yield the crude lithium salt of the acid (0.120 g).
• Step E (4-lsopropyl-piperazin-1 -yl)-(5-piperidin-1 -ylmethyl-pyrazin-2-yl)- methanone.
• Step A 5-Morpholin-4-ylmethyl-pyrazine-2-carboxylic acid methyl ester.
• the title compound was prepared in a manner similar to that described in Example
• Step C using 5-formyl-pyrazine-2-carboxylic acid methyl ester (0.78 g, 4.7 mmol) and morpholine (0.44 g, 5.2 mmol).
• Step B 5-Morpholin-4-ylmethyl-pyrazine-2-carboxylic acid. Hydrolysis of 5- morpholin-4-ylmethyl-pyrazine-2-carboxylic acid methyl ester (0.28 g) was performed in a similar manner to that described in Example 23, Step D, to give the crude lithium salt of the acid (0.224 g).
• Step C (4-lsopropyl-piperazin-1 -yl)-(5-morpholin-4-ylmethyl-pyrazin-2-yl)- methanone.
• the title compound (0.022 g, 7%) was prepared in a manner similar to that described in Example 23, Step E.
• Step A 3-(3-Piperidin-1-yl-propoxy)-isoxazole-5-carboxylic acid methyl ester.
• 3-Hydroxy-isoxazole-5-carboxylic acid methyl ester (0.859 g., 6.00 mmol) and 3-piperidin-1 -yl-propan-1 -ol (0.860 g., 6.00 mmol) were dissolved in DCM (30 mL), and polymer-supported Ph 3 P resin (3 mmol/g, 3.1 g, 9.30 mmol) was added.
• Step C 1 -[3-(5-Chloromethyl-isoxazol-3-yloxy)-propyll-piperidine.
• a mixture of [3-(3-piperidin-1-yl-propoxy)-isoxazol-5-yl]-methanol (0.150 g, 0.625 mmol) and neat thionyl chloride (4 mL) was heated at reflux for 3 h.
• the reaction mixture was cooled to rt and concentrated to give the desired chloride, which was carried to the next step without further purification.
• Step P 4-[3-(3-Piperidin-1 -yl-propoxy)-isoxazol-5-ylmethyll-piperidine.
• 1 -[3-(5- Chloromethyl-isoxazol-3-yloxy)-propyl]-piperidine (0.15 g, 0.80 mmol) was dissolved in PCM (5 mL) and piperidine (0.24 mL, 2.4 mmol) was added slowly. The reaction mixture was stirred overnight at rt and then was concentrated to yield the crude product (0.140 g).
• the crude product was purified (Si0 : 0-5% 2 M NH 3 in MeOH/PCM) to give the final product (0.118 g, 47.6%).
• the cells were diluted with 10 mL of complete media and were plated onto four 10 cm dishes at the following ratios: 1 :20, 1 :10, 1 :5, and 1 :2. The cells were allowed to recover for 24 h before adding 600 ⁇ g G-418. Colonies that survived selection were grown and tested. SK-N-MC cells were used because they give efficient coupling for inhibition of adenylate cyclase. The clones that gave the most robust inhibition of adenylate cyclase in response to histamine were used for further study.

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