WO2005044210A1

WO2005044210A1 - Recalcification promoter, oral composition, food and drink

Info

Publication number: WO2005044210A1
Application number: PCT/JP2004/013622
Authority: WO
Inventors: Shuji Sakuma; Kiminori Atsumi
Original assignee: Kabushiki Kaisha Sangi
Priority date: 2003-11-11
Filing date: 2004-09-17
Publication date: 2005-05-19
Also published as: JPWO2005044210A1; JP4324591B2

Abstract

[PROBLEMS] To provide an oral composition that can effectively promote the recalcification of tooth enamel decalcified at its surface and can positively suppress dental caries. [MEANS FOR SOLVING PROBLEMS] There is provided a recalcification promoter for tooth enamel comprising conchiolin and/or hydrolyzed conchiolin as an effective ingredient to which a calcium compound, especially preferably hydroxyapatite, is added.

Description

Specification

Remineralization accelerator, Oral composition, food, beverage

Technical field

The present invention relates to a tooth mineral enamel remineralization accelerator, an oral composition, a food and a beverage produced by adding the remineralization accelerator.

Background art

[0002] Dental decay begins when the dental caries bacteria adhere to the tooth surface and form dental plaque, and the acid produced by the dental caries metabolizing food in the dental plaque demineralizes tooth enamel. As a result, an initial corrosion state occurs. Saliva remineralizes this demineralized part by the action of calcium and phosphorus in saliva, and has the function of returning the teeth to their original state.

In order to promote remineralization, dentifrices containing hydroxyapatite, which is a type of fluoride or calcium phosphate and has a crystal structure similar to the inorganic components of teeth, have been manufactured.

[0003] However, remineralization of the demineralized portion is not sufficient only with the use of saliva, fluoride, or hydroxyapatite dentifrice. In addition, in order to promote remineralization, xylitol and calcium phosphate, non-crystalline calcium phosphate, chewing gum containing calcium phosphated oligosaccharide, etc. are manufactured. In the case of chewing gum containing calcium phosphate, remineralization of the demineralized part is not always sufficient.

[0004] By the way, concholine is composed of 17 kinds of amino acids such as glycine, alanine, serine, leucine, aspartic acid, valine, proline, isoleucine, tyrosine, arginine, glutamic acid, threonine, lysine, phenylalanine, methionine, histidine and cystine. A protein contained that has properties similar to collagen fibers found in human skin, has excellent affinity for skin and hair, and is a powerful moisturizing and moisturizing component, and also gives the skin a gentle and refreshing feel. Used in cosmetics.

[0005] Conkyorin is a protein contained in shells such as pearl oysters, white clams and black oyster clams, and pearls. About 90% of these pearls are calcium carbonate called aragonite, 5% of concholine, and 5% of others. It consists of the composition. The pearl is calcium carbonate The crystal and concholine are made to be stacked in multiple layers, and the luster and beautiful color tone peculiar to pearls are produced by the light interference effect.

Concholine powder can be obtained by removing acid by adding acid to a finely divided shell or pearl of shells such as pearl oysters, white-necked pearl clams, and black oyster clams, and then removing concholine. A hydrolyzed product of the concholine protein obtained by further hydrolyzing this concholine is hydrolyzed concholine.

[0006] The above concholine powder and Z or hydrolyzed concholine are dissolved or dispersed in a suitable liquid, or mixed with or adsorbed on a suitable powder carrier, and optionally an emulsifier, A dispersing agent, a suspending agent, a spreading agent, a penetrating agent, a wetting agent, a stabilizing agent, and the like can be added, and the composition can be formulated into an emulsion, a wettable powder, a powder, or a tablet and used.

[0007] In addition, it has been found that the use of concholine as a food can be expected to have an effect on skin gloss and astringency, an antioxidant effect, an inhibitory effect on generation of active oxygen, and a histamine inhibitory effect. Is disclosed in JP-A-8-173091. In addition, it has been found that the incorporation of a concholine hydrolyzate into a food has an effect of improving liver function, and the “food” is disclosed in JP-A-2003-24008.

Patent Document 1: JP-A-8-173091

Patent Document 2: Japanese Patent Application Laid-Open No. 2003-24008

Disclosure of the invention

Problems to be solved by the invention

[0009] The present invention provides an oral composition having an excellent remineralizing effect capable of effectively promoting remineralization of demineralized enamel and thereby actively inhibiting dental erosion. It is intended to provide food and beverages.

Means for solving the problem

[0010] In the present invention, remineralization of teeth is promoted by using concholine powder and / or hydrolyzed concholine, and by using these together with a calcium compound, remineralization of teeth is further reduced. The inventors have found that it is accelerated, and have accomplished the present invention.

The oral composition, food and beverage of the present invention contain the above-mentioned tooth enamel remineralization accelerator. It is manufactured by adding soy sauce. It has been found that the use of concholine powder and / or hydrolyzed concholine promotes remineralization of teeth, and that the use of these together with calcium compounds further promotes remineralization of teeth. The present inventors have found that the remineralization of teeth is further promoted by using hydroxyapatite in combination with lymphoda, Z or hydrolyzed conchioline, and accomplished the present invention.

[0011] The above-mentioned concholine powder and / or hydrolyzed concholine have excellent affinity for skin and hair as cosmetics, and as a powerful moisturizing component and moisturizing component, and have a gentle and refreshing feel to the skin. In addition, foods have been known to have effects on skin gloss and astringency, antioxidation effects, active oxygen generation inhibition effects, histamine suppression effects, liver function improvement effects, etc. It has been found that those containing lymphoid powder and / or hydrolyzed conchiolin ゃ, concholine powder and / or hydrolyzed conchioline and a calcium compound as active ingredients have the effect of promoting remineralization of tooth enamel. It has been found that oral compositions, foods and beverages to which a calcification accelerator has been added have an effect of promoting remineralization of tooth enamel.

[0012] Calcium compounds used in oral compositions, foods and drinks are obtained by crushing natural hard tissues such as bones and eggshells of fish, amphibians, reptiles, birds or animals belonging to mammals inhabiting seawater or freshwater. Powder, calcium chloride, calcium carbonate, calcium lactate, calcium dalconate, hydroxyapatite, tricalcium phosphate, citric acid phosphate, calcium hydrogen phosphate, calcium hydrogen phosphate dihydrate, calcium oxide, etc. . Particularly, hydroxyapatite, which is a kind of calcium phosphate, is preferable.

[0013] Hydroxyapatite crystals constituting the enamel on the tooth surface are composed of calcium phosphate and are considered to be effective as a supply source of calcium and phosphoric acid.

In addition, calcium compounds that are abundantly contained in the natural hard tissue as described above are used in the present invention in addition to those synthesized by ordinary methods. Fish bones, pork bones, cow bones and the like of edible fish such as.

[0014] A remineralization accelerator, an oral composition, a food, a beverage, and a combination of concholine powder and hydrolyzed concholine and a calcium compound, which contain the above concholine powder and Z or hydrolyzed concholine. Remineralization accelerators, oral compositions, foods, beverages, In particular, it was found that remineralization can be remarkably promoted by using concholine powder, or foods and beverages using hydrolyzed conchioline and hydroxyapatite in combination.

[0015] Examples of the oral composition of the present invention include dentifrices such as toothpaste, powdered toothpaste, and liquid toothpaste, rinsing lj, lozenges, troches, and chewing gums. , Sweets such as tablet confections, gummy jelly, chocolate, biscuits, snacks, etc., ice creams, sorbets, frozen desserts such as frozen desserts, beverages, breads, hot cakes, dairy products, hams, sausages, etc. Fish meat products, side dishes, pudding, soups, jams, etc.

[0016] The added amount of concholine powder and / or hydrolyzed concholine to the oral composition, food, or beverage, the amount of calcium compound added, and the amount of added calcium compound and hydroxyapatite can be determined depending on the form of the composition. The amount of concholine powder and / or hydrolyzed concholine is preferably 0.00001% by weight or more, and the amount of calcium compound or hydroxyapatite is preferably 0.001 to 50.0% by weight. More preferably, the addition amount of concholine powder and / or hydrolyzed concholine is 0.0001 to 80.0% by weight, and the addition amount of a calcium compound or hydroxyapatite is 0.01 to 30.0% by weight.

Of course, it is possible to mix only one of the concholine powder and / or hydrolyzed concholine components, or a combination of two, and it is possible to mix two or more of the calcium compounds with only one. It is.

[0017] In the present invention, as a method of adding a conoline powder and / or a hydrolyzed concholine, and further a calcium compound to the composition as necessary, a method of manufacturing the product may be used. Alternatively, the remaining raw materials may be mixed.

In the manufacture of oral compositions, foods and beverages to which concholine powder, hydrolyzed concholine, and a calcium compound are added, they may be mixed in advance and added to the mixture. In other words, concholine powder, hydrolyzed concholine, and potassium compound may be added separately.

[0019] The oral composition, food, and beverage of the present invention include, in addition to the components described above, an oral composition, a food It can contain additives, wetting agents, foaming agents, flavors, sweeteners, preservatives, various active ingredients, and the like, which are commonly used in products and beverages. Specific examples of these components are shown below.

Abrasives: calcium carbonate, calcium hydrogen phosphate, calcium pyrophosphate and the like.

Moisture 'j: glycerin, propylene glycol, polyethylene glycol and the like.

Blowing agents: sodium lauryl sulfate, N-lauroyl sarcosine sodium, nonionic surfactants, etc.

Thickeners: hydroxyethyl cellulose, sodium carboxymethyl cellulose, carrageenan, carboxybutyl polymer, chitan sun gum and the like.

Preservatives: paraoxybenzoate, alkyldiaminoethyldaricin hydrochloride, methylparaben, ethylparaben, sodium benzoate and the like.

Flavors: menthol, spearmint oil, lemon oil, you-cutting oil, etc.

Sweetener: saccharin sodium, stevia extract, aspartame and the like.

Other medicinal ingredients: allantoin, tocopherol acetate, isopropylphenol, β-glycyrrhetinic acid, triclosan, chlorhexidine, dextranase, chlorophyll, flavonoids, tranexamic acid, hinokitiol and the like.

[0021] The amounts of these optional components are appropriately used within a pharmaceutically acceptable range without impairing the effects of the present invention.

Examples of the present invention and test examples are described below.The following examples describe examples used for a remineralization test, and the scope of the present invention is limited thereby. is not.

The invention's effect

[0022] The remineralization accelerator of the present invention contains concholine powder, hydrolyzed concholine, calcium compound, and hydroxyapatite as active ingredients, and is manufactured by adding the remineralization accelerator described above. All of the oral compositions, foods and beverages of the present invention are advantageous for effectively promoting remineralization of demineralized tooth enamel and actively inhibiting dental erosion. The “control surface” in the figure is a part for comparing the degree of the remineralization effect of the “oral composition, food, beverage” in Examples and Comparative Examples. It is a state that maintains the state of "carious". Artificial caries (3.5 x 3. Omm window) area Of which, it is a half part.

Brief Description of Drawings

FIG. 1 is a photograph showing a control surface and a treated surface of a crown by a contact microradiogram (CMR).

FIG. 2 is a drawing in which the photograph shown in FIG. 1 is drawn using dark ink and the explanation of the state of each part is applied Q.

[0024] The "processing surface" in the figure refers to a portion where the specimens of Examples and Comparative Examples acted as follows.

(1) To prepare test substances, for dentifrices and drinks, use a suspension solution of artificial saliva as a test solution, and for troches, chewing gum, candy, jelly, etc., pulverize finely and dissolve in water from each test substance. A test solution was obtained by extracting a sex component and forming a suspension with artificial saliva.

(2) In the remineralization acceleration test, an artificial initial caries specimen was immersed in each test solution for 24 hours.

BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, the present invention will be described in detail with reference to examples.

A 3% aqueous solution of hydrolyzed conchiolin was used.

[Table 1]

Oral composition kneading * abrasive

[Table 2] i-chamber composition toothpaste

[Table 3]

Oral composition Toothpaste

[0029] [Table 4]

Oral composition Liquid dentifrice

[Table 5] Oral composition

[0031] [Table 6

Food, beverage chewing gum

[Table 7]

Food, beverage candy

[0033] [Table 8] Food and beverages

[0034] [Table 9

Beverage

[Remineralization Acceleration Test Method]

In order to confirm the effect of promoting remineralization, an artificial initial caries test sample prepared in advance was used.

An artificial initial caries test sample was prepared by removing the dirt and deposits on the enamel surface using the crown of a human extracted tooth that had not been subjected to dental preservation and prosthesis treatment. Create a 3.5 x 3. Omm window with Nail Enamel for the test target site, and place it in 0.1 M lactate buffer (pH 4.5, 3. Omm CaCl, 1.8 mm KHPO, 0.5% CMC). . C, soak for 7 days

2 2 4

It was immersed to create an artificial initial caries. As a control for the test, half of the 3.5 x 3. Omm window on the crown apical side was further masked with Nail Enamel, and used as a comparison target (control).

[0036] The test substance was prepared by adjusting a dentifrice, a liquid dentifrice, a liquid dentifrice, or a beverage in the form of a suspension solution with artificial saliva as a test solution. After pulverization, a water-soluble component was extracted from each test substance, and a suspension with artificial saliva was used as a test solution. [0037] In the remineralization acceleration test, the artificial initial caries specimen prepared above was immersed in each test solution for 24 hours, and then the specimen was parallelized to the tooth axis with a micro cutter. The piece was cut to a thickness of about 50 Oxm, and then the section was polished using a kneading whetstone and a natural whetstone under water injection so as to become a parallel thin section of about 100 xm thickness. After polishing, contact microradiogram (CMR) imaging was performed to confirm the remineralization effect of the teeth (see Fig. 1 and Fig. 2). The analysis was performed using a computer.

[0038] Computer image analysis is based on the formula of Angmer et al. (B. Angmer, D. Carlstrom and

JE. Glas: Studies on Ultrastructure of Dental Enemel IV: The Mineralization of normal Human Enamel, J. Ultrastructure. Res. 8, 12-23, 1963) According to the method (FA Damato, R. Stang and KW Stephen: Effect of Fluoride Concentration on Reminerelization of Carious Enamel: an in vitro pH-Cycling Study, Caries Res, 24, 174-180, 1990) The amount of mineral loss AZ (% volume mineral · / im) on the treated surface was calculated. The remineralization rate was calculated by the following formula.

[Equation 1] Remineralization rate = Control port- ^Δの of treatment surface- ^{Δ Δ of} treatment surface 1 0 0 (ο / ₀ )

Δ の on the control surface

[0040] Table 10 shows the results of confirming the effect of promoting the remineralization of the oral composition by the remineralization acceleration test method described above.

[Table 10] Example Comparison

* Polishing 磨 Example 1 Example 2 (Example 3 Example 4 Example 5 Remineralization rate «) 1.4 16.5 11,8 17.3 10.1 5.2 Example 'j ¾te' M7

Toothpaste 10

Remineralization rate «) 14.1 26.7 19, 19.6 26.5 29.8 m Implemented 俩 Implemented 俩

i

Toothpaste 11 12

Remineralization rate (¾) 7.8 14.2 22.5

Example

Example Comparative example 4

Liquid dentifrice 13 14

Remineralization rate «) 0.9 6.8 29,6

Example Example *

Example Comparison 俩 5

Liquid dentifrice 15 16

Remineralization rate (¾) 8.0 22.0 61, 7

Implementation 俩 Implementation 俩 Implementation

Example Comparison

Lozenge 17 18 19

Remineralization rate (¾) 9.7 27.1 20.4 23.2

[0042] Table 11 shows the results of confirming the effect of promoting remineralization of foods and beverages by the aforementioned remineralization acceleration test method.

[Table 11]

Chewing gum Example Comparative Example 7 Example 20 Example 21

Remineralization rate (%) 1, 1 7.4 8.7

Chewing gum Example Comparative Example 8 Example 22 Example 23

Remineralization rate (%) 7.3 17.7 31.1

Canry Example Comparative 'Example 9: Poultry Example 24-Example 25 Example 26 Ma f' 化 ^ (%) 10.4 18, 2 37.9 26.0 Jelly ― Example Comparative Example 10 Example 27 ^ Example 28

Calcification rate (%) 4, 6 8.3 15.8

Example Comparative Example 11 Example 29 Example 30

Beverage

Remineralization rate (%) 5, 2 56.2 43.5

Claims

The scope of the claims

[1] An agent for promoting remineralization of tooth enamel, characterized by containing concholine powder and / or hydrolyzed concholine.

[2] The remineralization promoting agent for tooth enamel according to claim 1, wherein a calcium compound is blended.

[3] The agent for promoting remineralization of tooth enamel according to claim 2, which is a calcium compound or hydroxyapatite.

[4] Calcium compound power Natural bone tissue powder, calcium chloride, calcium lactate, calcium dalconate, tricalcium phosphate, calcium citrate, calcium hydrogen phosphate, calcium hydrogen phosphate dihydrate, calcium oxide The remineralization promoting agent for tooth enamel according to claim 2, characterized in that one or more of the following are blended.

[5] An oral composition, food, or beverage produced by adding the tooth enamel remineralization promoter according to any one of claims 1 to 3.

[6] An oral composition, food, or beverage produced by adding a remineralization accelerator, wherein the calcium compound according to claim 2 is hydroxyapatite.