WO2005023210A1

WO2005023210A1 - Recalcification-promoting agent, oral composition, food and drink

Info

Publication number: WO2005023210A1
Application number: PCT/JP2003/011336
Authority: WO
Inventors: Shuji Sakuma; Tomoki Saito; Tsutomu Ishizaki
Original assignee: Kabushiki Kaisha Sangi
Priority date: 2003-09-05
Filing date: 2003-09-05
Publication date: 2005-03-17

Abstract

It is intended to provide an oral composition, a food and a drink which effectively promote the recalcification of decalcified tooth enamel and thus positively prevent tooth decay. An agent for promoting recalcification contains, as the active components, a protein extracted from a plant and a calcium compound. The above-described oral composition, food and drink are produced by adding the above components or adding a recalcification-promoting protein and a calcium compound separately.

Description

Description Remineralization accelerator, Oral composition, Food and beverage

The present invention relates to a remineralization accelerator for tooth enamel, an oral composition, a food and a beverage produced by adding the remineralization accelerator. Background art

ゥ The insectivorous insects are Streptococcus mutans ゃ Streptococcus sobrinus and other oral streptococci (cariogenic bacteria) adhere to the tooth surface, and these bacteria produce insoluble glucan. It begins with the formation of plaque. In the plaque, the acid produced by the bacteria metabolizing food demineralizes tooth enamel, resulting in a so-called initial caries state.

Saliva has the effect of restoring the teeth to their original state by remineralizing the demineralized part. Dentifrices have also been developed that attempt to remineralize the tooth surface by blending microparticles of hydroxyapatite with a crystal structure similar to the inorganic components of teeth. However, hydroxyapatite is a chemically stable compound and has poor reactivity. Hydroxyapatite used in such dentifrices is manufactured industrially and, strictly speaking, has a different crystal structure from the hydroxyapatite that constitutes teeth in living organisms. Is not enough. Disclosure of the invention

The present invention is intended to effectively promote remineralization of demineralized tooth enamel. Both are intended to provide oral compositions and foods and drinks having an excellent remineralization effect by which the erosion can be positively suppressed. The present inventors have obtained the finding that the above object can be achieved by using a plant extract protein and a calcium compound in combination, and have completed the present invention. The remineralization accelerator for tooth enamel according to the present invention contains a plant extract protein and a calcium compound as active ingredients. Soybean extracted protein is preferable as the above-mentioned plant-extracted protein, and calcium phosphates such as hydroxyapatite are preferable as the calcium compound.

The oral composition and the food and drink of the present invention are produced by adding the above-mentioned tooth enamel remineralization accelerator.

In the above description, “plant-extracted protein” refers to a protein or a defatted product thereof, or a protein such as beans or cereals that has been processed to increase the protein content. The plant protein may be in any form such as powder, granule, paste, and fiber. The seeds for oil extraction include, for example, castor, rape, sesame, peanuts, almonds, walnuts and the like. Beans include soybean, azuki, coffee, cowpea, fava bean, kidney bean, chickpea, ryokuto, ryamame and the like. Examples of cereals include wheat, corn, rice, red pepper, barley, sopa, millet, notogi, hee, aye, rye. In addition, leaf materials such as tea leaves and bamboo grasses may be mentioned.

In the above, the “calcium compound” refers to a powder obtained by crushing natural hard tissues such as bones and eggshell shells of fish, amphibians, reptiles, birds and mammals belonging to seawater or freshwater, calcium chloride, calcium carbonate , Calcium lactate, calcium dalconate, hydroxyapatite, tricalcium phosphate, calcium citrate, calcium hydrogen phosphate, calcium hydrogen phosphate dihydrate, calcium oxide and the like. Especially high Calcium phosphates such as droxyapatite, tricalcium phosphate and calcium hydrogen phosphate dihydrate are preferred.

Hydroxyapatite crystals, which constitute the enamel on the tooth surface, are composed of calcium phosphate and are considered to be an effective source of calcium and phosphoric acid. As the above-mentioned natural hard tissues, those rich in calcium compounds are used in the present invention, but the most useful ones include fish bones, pork bones, and cow bones of edible fish such as salmon. . It has been found that a remineralization accelerator using the above-described plant extract protein and a calcium compound in combination can be used in an oral composition or food or beverage to remarkably promote remineralization.

The processing for obtaining the plant protein used in the present invention is, for example, appropriately combining steps such as dehulling, crushing, degreasing, water extraction, extraction with an aqueous solvent, acid precipitation, washing, centrifugation, dialysis, and drying. It depends on the plant used. Each step can be performed by a conventional method for each plant. In the case of soybean, for example, soybean protein is extracted by sequentially performing the steps of dehulling, grinding, delipidation, extraction with water or a buffer, acid precipitation, centrifugation, dialysis, and lyophilization.

Further, the above-mentioned plant extract protein is dissolved or dispersed in a suitable liquid carrier, or mixed with or adsorbed to a suitable powder carrier, and optionally, an emulsifier, a dispersant, or a suspending agent. , A spreading agent, a penetrating agent, a wetting agent or a stabilizer, and the like can be added to prepare an emulsion, a wettable powder, a powder, a tablet or the like for use.

Examples of the oral composition of the present invention include toothpastes such as toothpaste, powdered toothpaste, and liquid toothpaste, mouthwashes, gum massage creams, gargle tablets, troches and the like.

The oral composition of the present invention can be used by a dentist or a dental hygienist to mechanically treat the tooth surface. It can also be used as an abrasive for polishing or cleaning. Examples of the above-mentioned method for mechanically polishing or cleaning teeth include a scaler, an ultrasonic scaler, a polishing brush, a polishing cup, a profiling mold piece, an adder, an eva chip, and the like. It can be used as a tooth abrasive when using equipment or appliances. In recent years, “PMTC” (Professional Mechanical Tooth Cleaning) has attracted attention as a mechanical plaque control method for preventive dentistry, dental maintenance, and oral care. The PMTC includes a step of physically removing mutated plaque (hereinafter referred to as “biofilm”) and a step of cleaning and polishing the tooth surface. In addition, the oral composition of the present invention is suitable for use as an abrasive.

Conventionally, in the above-mentioned PMTC, an abrasive such as silica or alumina has been used for removing the biofilm on the tooth surface and polishing the tooth surface. In spite of the problem that the wound is easily damaged and the tooth enamel is damaged, the damage to the tooth surface enamel can be reduced by using the oral composition of the present invention as an abrasive. In recent years, there has been a bleaching method for living teeth in which carbamide peroxide is placed in a vinyl tray to restore the aesthetics of discolored teeth.However, surface enamel immediately after bleaching teeth with carbamide peroxide is used. The problem is that the quality is highly carious. Thus, by using the oral composition of the present invention as an abrasive for cleaning the tooth surface, the degree of calcification of enamel in the tooth surface layer can be increased, and the incidence of dental caries can be reduced. Food or beverages include sweets such as chewing gum, candy, tablet confectionery, gummy jelly, chocolate, biscuits, snacks, etc. Examples include meat products such as ham and sausage, fish products such as potato mackerel and chiku, prepared foods, puddings, soups and jams.

The amount of the plant-extracted protein or calcium compound to be added to the oral composition or the food / drink is difficult to determine unconditionally depending on the form of the oral composition or the food / drink, etc. It is preferably from 1 to 50.0% by weight, from 0.01 to 50.0% by weight.

In the present invention, as a method for adding a plant extract protein or a calcium compound to an oral composition or food or drink, it may be added at any time during the production process of the product, or may be mixed with the remaining raw materials. In the production of an oral composition or a food or drink to which a plant extract protein and a calcium compound have been added, they may be added in advance by mixing them, or separately, that is, the plant extract protein and the calcium compound may be added separately. It may be added separately. Hereinafter, examples and test examples of the present invention will be described, but the scope of the present invention is not limited thereby.

The remineralization accelerator of the present invention contains a plant extract protein and a calcium compound as active ingredients, and the oral composition, food and beverage of the present invention produced by adding the remineralization accelerator described above include: Both are effective in effectively promoting the remineralization of demineralized tooth enamel and actively inhibiting dental erosion. The `` control surface '' in the attached figure is used to compare the degree of remineralization effect of each subject such as `` chewing gum, soft confectionery in the mouth '' in Examples and Comparative Examples. It is the part that maintains the state of "artificial initial caries". Half of the artificial caries (the “3.5 x 3.0 mm window” area).

The “processed surface” in the attached drawing is a portion where the specimens of the example and the comparative example acted as follows.

(1) Adjust the test substance for chewing gum, soft drinks, etc. After pulverization, water-soluble components were extracted from each test substance and used as a test solution. A suspension of dentifrice in water was used as a test solution, and a mouthwash was used as a test solution.

(2) In the remineralization acceleration test, artificial initial caries specimens were immersed in each test solution for 24 hours. Brief Description of Drawings

Figure 1 is a photograph showing the control surface and the treated surface of the crown by contact microradiogram (CMR).

FIG. 2 is a diagram in which the photograph shown in FIG. 1 is drawn using dark ink and an explanation of the state of each part is added. BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, the present invention will be described in detail with reference to examples.

[Example 1] (Production of soybean protein)

Soybean as a soybean protein extraction raw material was dehulled, ground using a mixer, and defatted with 5 times the weight of hexane. The defatted soybeans were stirred for 1 hour with 20 times the weight of 0.03 M Tris-HC1 buffer (pH 8.0) containing 0.01 M 2-mercaptoethanol, 18,000 Xg, After centrifugation at 20 ° C for 20 minutes, the supernatant was adjusted to pH 6.4 with 2N HC1, and centrifuged at 18,000 X g at 4 ° C for 20 minutes. The precipitate was dispersed in distilled water, ammonium sulfate was added to a saturated concentration, and the mixture was centrifuged at ll, 00OX g and 4 ° C for 20 minutes. The precipitate was dialyzed against distilled water and freeze-dried to obtain a soybean protein.

[Examples 2 and 3] (chewing gum)

Hydroxyapatite and protein extracted from soybeans as plant-extracted protein The chewing gums of Examples 2 and 3 and Comparative Example 1 of the present invention were prepared by using the composition as shown in Table 1. table 1

(The figures are% by weight)

[Example 4] (chewing gum)

Using dibasic calcium phosphate and a protein extracted from soybeans as a plant-extracted protein, chewing gums of Example 4 and Comparative Example 2 of the present invention having the formulations shown in Table 2 were produced. Table 2

(The figures are% by weight) [Examples 5 and 6] (Mouth refreshing sweets)

Using hydroxyapatite and a protein extracted from sunflower as a plant-extracted protein, refreshing confections in the mouth of Examples 5 and 6 of the present invention and Comparative Example 3 were prepared according to the formulations shown in Table 3. Table 3

(The figures are% by weight)

[Examples 7, 8] (Mouthwash)

Using hydroxyapatite and soybean-extracted proteins as plant-extracted proteins, the mouthwashes of Examples 7 and 8 of the present invention and Comparative Example 4 having the formulations shown in Table 4 were prepared. Table 4

(The figures are% by weight) [Examples 9, 10] (Toothpaste composition)

Toothpaste compositions of Examples 9, 10 and Comparative Example 5 of the present invention were prepared using the blends shown in Table 5 by using tricalcium phosphate and dibasic calcium phosphate and soybean extracted protein as the plant extracted protein. Table 5

(The figures are% by weight)

[Examples 11 and 12] (Toothpaste composition)

Using hydroxyapatite, dibasic calcium phosphate, and soybean extracted protein as plant-extracted proteins, the toothpaste compositions of Examples 11 and 12 of the present invention and Comparative Example 6 were prepared according to the formulations shown in Table 6. did.

Table 6

(The figures are% by weight.) [Example 13] (Beverage)

Kuen acid 0.1 5 wt ^_0/0, Kuen trisodium 0.02 wt%, scan Tebia sweetener 0.04 wt%, was mixed with lemon flavor 0.1% by weight, the total amount by adding water 100% by weight. To this, 0.01% by weight of the protein extract of the present invention and 0.01% by weight of calcium lactate were added to obtain a beverage of the present invention.

[Example 14] (Beverage)

After mixing 1.2% by weight of acidity, 5.0% by weight of xylitol and 0.3% by weight of fragrance, water was added to make the total amount 100% by weight. This sesame extract protein 0.1 wt ^_0/0及Pi calcium lactate 0.1 wt ^_0/0 of the present invention with added pressure to give the beverage of the present invention.

[Example 15] (Beverage)

Kuen acid 0.1 5 wt ^_0/0, trehalose 10.0 wt ^_0/0, after mixing the perfume 0.2% by weight was 1 00 wt% of the total amount by adding water. Book on this The beverage of the present invention was obtained by adding 0.05% by weight of the walnut extract protein of the present invention, 0.01% by weight of calcium citrate and 0.01% by weight of hydroxyapatite.

[Example 16]

Table 7 shows examples of tooth surface abrasives. Table 7

As a result of observation by SEM, it was found that the soy protein-containing tooth polishing agent smoothed the tooth surface and had an excellent recrystallization effect on the enamel surface.

[Remineralization accelerated test method]

In order to confirm the effect of promoting remineralization, an artificial initial caries test sample prepared in advance was used. An artificial initial caries test sample was prepared using the crown of an extracted tooth of a human without dental preservation and prosthesis treatment, after removing enamel surface deposits and deposits. the tested site on the surface creates a window of 3. 5 X 3. 0 mm in Nail Enamel, 0. 1 M lactate buffer solution (pH 4. 5, 3. 0 mm C a C l 2, 1. 8 mm KH ₂ P0 _4, by immersing 37 ° C, 7 days 0. 5% CMC), to prepare an artificial initial caries It was. As a control for the test, half of the crown crown side of the 3.5 x 3.0 mm window was further masked with Nail Enamel to obtain a comparison target site (control).

For the preparation of test substances, chewing gum, soft confectionery in the mouth, etc. are finely pulverized, and then water-soluble components are extracted from each test substance to form a test solution.Toothpaste is a suspension solution of water in the test solution. The test solution for the mouthwash was used as it was.

In the accelerated remineralization test, the artificial initial caries test specimen prepared above was immersed in each test solution for 24 hours, and then the specimen was moved with a micro cutter so that it was approximately 5 parallel to the tooth axis. The slice was cut to a thickness of 0 μμπι, and then the slice was polished using a kneading whetstone and a natural whetstone under water injection so as to obtain a parallel thin slice of about 10 μμπι thickness. After polishing, contact microradiograms (CMR) were taken to confirm the remineralization effect of the teeth (see Figs. 1 and 2), and a computer was used to evaluate the effect of remineralization of the artificial initial carious site. The analysis was performed using

Image analysis by computer is based on the formula of Angmer et al. (B. Angmar, D. Carlstrom and d. E. Glas: Studies on Ultrastruct re of Dental Enamel IV: The Mineralization of normal Human Enamel, J. Ultrastructure. Res. 8, 12-23, 1963) and calculated the amount of minerals remineralized by the method of Damato et al. (FA Damato, R. Stang and KW Stephen: Effect of Fluoride Concentration on Remineralization of Carious Enamel '■ an in vitro pH- Cycling Study, Caries Res, 24, 174-180, 1990), the amount of mineral loss Δ Ζ (% volume mineral-μπι) on the control and treated surfaces of each section was calculated. The remineralization rate was calculated by the following formula. ! 0 0 (%)

Table 8 shows the results of confirming the effect of promoting remineralization by the above-described remineralization acceleration test method.

Examples 2 and 3 and Comparative Example 1 are all chewing gums and have a common component composition other than purified water and soybean extracted protein. Comparing the remineralization rates in Table 8, it can be seen that both Examples 2 and 3 and Comparative Example 1 according to the present invention have a remineralization effect, but both Examples 2 and 3 are compared. It can be seen that it is much better than Example 1; Example 2 is about 4.7 times higher than Comparative Example 1; and Example 3 is about 9 times higher than Comparative Example. In addition, it can be seen that Example 3 in which the content of the soybean protein was increased had a higher effect of promoting remineralization than Example 2.

The same effect as the above results is obtained from the chewing gum of Example 4 and Comparative Example 3, which share the same composition of components other than purified water and plant extract protein; And 8, the mouthwash of Comparative Example 4, the toothpaste compositions of Examples 9 and 10, the toothpaste composition of Comparative Example 5, the toothpaste compositions of Examples 11 and 12, and the toothpaste composition of Comparative Example 6, respectively. This can also be seen in the results of the remineralization promoting effect test (Table 8). That is, the results in Table 8 show that the oral compositions, foods and beverages according to the present invention are much more effective in effectively promoting the remineralization of demineralized tooth enamel. ing. Table 8

Retardation surface Re-calcification rate Mineral loss Mineral loss (%) mm Z (% volume mm Ζ (% volume

mineral- μπι mineral-μπι

Chewing gum Comparative example 1 7962.2 7749.3 2.67 Example 2 7803.7 6821.3 12.59 Example 3 7859.6 5979.1 23.93 Chewing gum Comparative example 2 6320.4 6175.3 2.30 Example 4 6103.7 5542.9 9.19 Oral soft confectionery Comparative example 3 7046.4 7025.8 0.29 Example 5 8.5 7143.8 65 7188.4 5988.5 16.69 Mouthwash Comparative Example 4 7194.3 7002.9 2.66 Example 7 7005.6 6451.8 7.91 Example 8 7088.9 6777.8 4.39 Toothpaste composition Comparative Example 5 7105.6 6698.1 5.73 Example 9 7009.5 5332.7 23.92 Example 1 0 7220.8 3204.7 55.62 Toothpaste Comparative Example 6 7455.6 6919.1 7.20 Example 1 1 7398.5 5764.6 22.08 Example 1 2 7551.2 3811.1 49.53

Claims

05/023210 15 Claims

1. A remineralization accelerator for tooth enamel, comprising a plant extract protein and a calcium compound as active ingredients.

2. The remineralization promoting agent for tooth enamel according to claim 1, wherein the plant-extracted protein is a liquid, powdery or pasty compound obtained by processing an edible plant.

3. The remineralization promoter for tooth enamel according to claim 1 or 2, wherein the plant-extracted protein is a soybean extract.

4. The calcium compound is a powder of natural bone tissue, calcium chloride, calcium carbonate, calcium lactate, calcium dalconate, hydroxyapatite, tricalcium phosphate, calcium citrate, calcium hydrogen phosphate, calcium hydrogen phosphate 2 2. The remineralization accelerator for tooth enamel according to claim 1, wherein one or more of hydrates and calcium oxide are blended.

5. A plant-extracted protein and a calcium compound as active ingredients, wherein the plant-extracted protein is a liquid, powdery or pasty compound obtained by processing an edible plant, and the calcium compound is a natural bone tissue. Of powder, calcium chloride, calcium carbonate, calcium lactate, calcium dalconate, hydroxyapatite, tricalcium phosphate, calcium citrate, calcium hydrogen phosphate, calcium hydrogen phosphate dihydrate, calcium oxide A remineralization accelerator for tooth enamel, characterized by containing one or more types.

6. An oral composition produced by adding the tooth enamel remineralization accelerator according to any one of claims 1 to 5.

7. A food produced by adding the tooth enamel remineralization promoter according to any one of claims 1 to 5.

8. A beverage produced by adding the tooth enamel remineralization accelerator according to any one of claims 1 to 5.

9. An abrasive for tooth surfaces comprising the oral composition according to claim 6.