Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/59—Compounds containing 9, 10- seco- cyclopenta[a]hydrophenanthrene ring systems
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
  • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]

Definitions

• This invention relates to vitamin D compounds, and more particularly to the use of 2-methylene-19-nor- vitamin D compounds to prevent and/or treat inflammatory bowel disease.
• the natural hormone, l ⁇ ,25-dihydroxyvitamin D3 and its analog l ⁇ ,25- dihydroxyvitamin D2 are known to be highly potent regulators of calcium homeostasis in animals and humans, and their activity in cellular differentiation has also been established, Ostrem et al., Proc. Natl. Acad. Sci. USA, 84, 2610 (1987).
• IBD Inflammatory bowel diseases
• IBD chronic recurring illnesses most commonly involving inflammation of the terminal ileum and colon, although these diseases can also affect many sites throughout the alimentary tract.
• genetic factors predispose individuals to development of IBD (Podolosky 1991).
• the environment contributes to IBD development, and there is reason to believe that vitamin D may be an environmental factor which affects IBD. Vitamin D from sunlight exposure is less in areas where IBD occurs most often, as IBD is most prevalent in northern climates such as North America and Northern Europe (Podolosky 1991,
• vitamin D results from its manufacture via a photolysis reaction in the skin, and vitamin D available from sunlight exposure is significantly less in northern climates, and especially low during the winter (Clemens et al. 1982, DeLuca 1993). Dietary intake of vitamin D is problematic since there are few foods which are naturally rich in vitamin D. Weight loss occurs in 65-75% of patients diagnosed with Crohn's disease and 18- 62% of patients with ulcerative colitis (Fleming 1995, Geerling et al. 1998). Vitamin deficiencies in general and vitamin D deficiency in particular have been shown to occur in IBD patients (Andreassen et al. 1998, Kuroki et al. 1993).
• vitamin D status could be an environmental factor affecting the prevalence rate for IBD and that the correlation warrants investigation.
• the identification of vitamin D receptors in peripheral blood mononuclear cells sparked the early interest in vitamin D as an immune system regulator (Bhalla et al. 1983, Prowedini et al. 1983).
• the CD4+ Th cells have vitamin D receptors and are therefore targets for vitamin D (Veldman et al. 2000).
• Hormonally active vitamin D (1,25-dihydroxycholecalciferol) suppressed the development of at least two experimental autoimmune diseases (Cantorna et al.
• Thl cytokines IL-2, IFN- ⁇ , and TNF- ⁇
• IL-2, IFN- ⁇ , and TNF- ⁇ Thl cytokines
• 1,25-dihydroxycholecalciferol treatment has been shown to suppress the development of other T cell mediated experimental autoimmune diseases (multiple sclerosis, and arthritis; Cantorna et al. 1996, Cantorna et al. 1998).
• Vitamin D supplementation of patients on corticosteroids has been shown to prevent steroid induced bone loss (Buckley et al. 1996).
• the hormonally active form of vitamin D (1,25-dihydroxycholecalciferol) is known to increase bone mineralization when given to experimental animals (Cantorna et al. 1998) and people (Ongphiphadhanakul et al. 2000).
• vitamin D and or 1,25-dihydroxycholecalciferol supplementation may be the maintenance of bone mineral density.
• the present invention provides new vitamin D analogs that can prevent and/or treat IBD.
• the present invention is thus directed toward a method of preventing inflammatory bowel diseases (IBD) in susceptible individuals and treating patients with IBD by administering an amount of a 2-methylene-19-nor-vitamin D compound, preferably either l ⁇ -hydroxy-2-methylene-19-nor-homopregnacalciferol (hereinafter referred to as "2-MP") or 2-methylene-19-nor-20(S)-l ⁇ ,25-dihydroxyvitamin D 3 (hereinafter referred to as "2-MD”), or a combination of both, effective to prevent IBD development or to diminish IBD symptoms, respectively.
• 2-MP l ⁇ -hydroxy-2-methylene-19-nor-homopregnacalciferol
• 2-MD 2-methylene-19-nor-20(S)-l ⁇ ,25-dihydroxyvitamin D 3
• 2-MP is substantially non- calcemic and 2-MD can be used at such a low dose that hypercalcemia does not result thus avoiding the primary disadvantage of l,25(OH) 2 D 3 .
• the method comprises selecting an IBD patient and administering a sufficient amount of the vitamin D analog to the patient such that the IBD symptoms are abated.
• the 2-methylene-19-nor-vitamin D compounds found useful to prevent and/or treat IBD are characterized by the general formula I shown below:
• Y j and Y2 which may be the same or different, are each selected f om the group consisting of hydrogen and a hydroxy-protecting group, and where the group R represents any of the typical side chains known for vitamin D type compounds. More specifically R can represent a saturated or unsaturated hydrocarbon radical of 1 to 35 carbons, that may be straight-chain, branched or cyclic and that may contain one or more additional substituents, such as hydroxy- or protected-hydroxy groups, fluoro, carbonyl, ester, epoxy, amino or other heteroatomic groups. Preferred side chains of this type are represented by the structure below
• stereochemical center (corresponding to C-20 in steroid numbering) may have the R or S configuration, (i.e. either the natural configuration about carbon 20 or the 20-epi configuration), and where Z is selected from Y, -OY, -CH2OY, -C ⁇ CY and -CEHCHY, where the double bond may have the cis or trans geometry, and where Y is selected from hydrogen, methyl, -COR-> and a radical of the structure:
• R s selected from hydrogen, deuterium, hydroxy, protected hydroxy, fluoro, trifluoromethyl, and C ⁇ _5-alkyl, which may be straight chain or branched and, optionally, bear a hydroxy or protected-hydroxy substituent
• each of R R ⁇ , and Bx, independently, is selected from deuterium, deuteroalkyl, hydrogen, fluoro, trifluoromethyl and C j _5 alkyl, which may be straight-chain or branched, and optionally, bear a hydroxy or protected-hydroxy substituent
• R* and R ⁇ taken together may represent an oxo group, or an alkylidene group or the group -(CH2)p-, where p is an integer from 2 to 5, and where R ⁇ and R ⁇ taken together represent an oxo group, or the group -(CH2)q-, where q is an integer from 2 to 5, and where R-> represents hydrogen, hydroxy
• side chains with natural 20R-configuration are the structures represented by formulas (a), b), (c), (d), (e), (f), (g) and (h) below, i.e. the side chain as it occurs in 25-hydroxyvitamin D3 (a); vitamin D3 (b); 25- hydroxyvitamin D2 (c); vitamin D2 (d); the C-24 epimer of 25-hydroxyvitamin D2 (e); pregnacalciferol (f); homopregnacalciferol (g); and bis-homopregnacalciferol (h).
• Vitamin D analogs such as but not limited to the following are particularly preferred: l ⁇ -hydroxy-2-methylene-19-nor-homopregnacalciferol (see formula II and referred to herein as "2-MP") and 2-methylene- 19-nor-20(S)-l ⁇ ,25-dihydroxyvitamin D 3 (see formula III and referred to herein as "2-MD").
• 2-MP 2-methylene- 19-nor-20(S)-l ⁇ ,25-dihydroxyvitamin D 3
• the above compounds may be present in a composition to prevent and/or treat IBD in an amount from about O.Ol ⁇ g/gm to about lOO ⁇ g/gm of the composition, and may be administered topically, transdermally, orally or parenterally in dosages of from about O.Ol ⁇ g/day to about lOmg/day per 160 pound person.
• a preferred dose of vitamin D compound for the present invention is that which is effective to prevent and/or treat IBD and further is the maximum that a patient can tolerate and not develop serious hypercalcemia. The preferred dose is between O.l ⁇ g and lOmg per day per 160 pound patient depending on the calcemic properties of the vitamin D analog used.
• the patient has calcium intakes of above 800 mg/day, and the vitamin D analog is calcemic (like l ⁇ ,25-dihydroxyvitamin D 3 ) doses over 2 ⁇ g per day per 160 pound patient are not preferred. If the patient is on a low calcium diet and/or takes the dose late at night, higher doses would be possible and may be preferred, especially if the compound is non-calcemic.
• the amount of compound administered could be as high as lOmg per day per 160 pound patient.
• a preferred dose would be 0.5 ⁇ g to lOmg per day per 160 pound patient for a non-calcemic compound and O.l ⁇ g/day to 50.0 ⁇ g/day for a compound that has a tendency to raise serum calcium.
• hydroxy-protecting group signifies any group commonly used for the temporary protection of hydroxy functions, such as for example, alkoxycarbonyl, acyl, alkylsilyl or alkylarylsilyl groups (hereinafter referred to simply as “silyl” groups), and alkoxyalkyl groups.
• Alkoxycarbonyl protecting groups are alkyl-O-CO- groupings such as methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, isopropoxycarbonyl, butoxycarbonyl, isobutoxycarbonyl, tert-butoxycarbonyl, benzyloxycarbonyl or allyloxycarbonyl.
• acyl signifies an alkanoyl group of 1 to 6 carbons, in all of its isomeric forms, or a carboxyalkanoyl group of 1 to 6 carbons, such as an oxalyl, malonyl, succinyl, glutaryl group, or an aromatic acyl group such as benzoyl, or a halo, nitro or alkyl substituted benzoyl group.
• alkyl as used in the description or the claims, denotes a straight-chain or branched alkyl radical of 1 to 10 carbons, in all its isomeric forms.
• Alkoxyalkyl protecting groups are groupings such as methoxymethyl, ethoxymethyl, methoxyethoxymethyl, or tetrahydrofuranyl and tetrahydropyranyl.
• Preferred silyl-protecting groups are trimethylsilyl, triethylsilyl, t- butyldimethylsilyl, dibutylmethylsilyl, diphenylmethylsilyl, phenyldimethylsilyl, diphenyl-t-butylsilyl and analogous alkylated silyl radicals.
• aryl specifies a phenyl-, or an alkyl-, nitro- or halo-substituted phenyl group.
• alkylidene refers to any of a series of unsaturated open-chain hydrocarbons of 1 to 10 carbons containing a double bond and corresponding in composition to the general formula
• hydroxyalkyl refers to an alkyl radical substituted by one or more hydroxy, deuterium or fluoro groups respectively.
• vitamin D compound refers to an alkyl radical substituted by one or more hydroxy, deuterium or fluoro groups respectively.
• 19-nor-vitamin D compounds refers to the compounds defined by general formula I. It should be noted in this description that the term “24-homo” refers to the addition of one methylene group and the term “24-dihomo” refers to the addition of two methylene groups at the carbon 24 position in the side chain. Likewise, the term “trihomo” refers to the addition of three methylene groups. Also, the term “26,27-dimethyl” refers to the addition of a methyl group at the carbon 26 and 27 positions so that for example R ⁇ and R4 are both ethyl groups.
• the term "26,27-diethyl” refers to the addition of an ethyl group at the 26 and 27 positions so that R ⁇ and R ⁇ are both propyl groups.
• a methylene substituent is attached at the carbon 2 position of the A-ring and thus the term “2-methylene” is used in the nomenclature.
• the term "19-nor” proceeds each of the named compounds.
• the term "20(S)" or "20-epi" should be included in each of the following named compounds.
• the named compounds could also be of the vitamin D2 and/or D 4 type if desired.
• Specific and preferred examples of the vitamin D compounds of structure I when the side chain is unsaturated are: 2-methylene- 19-nor- 1 ⁇ -hydroxy-22-dehydrovitamin D 3 ; 2-methylene- 19-nor- 1 ,25-dihydroxy-22-dehydrovitamin D 3 ; 2-methylene- 19-nor- 1 ,24-dihydroxy-22-dehy drovitamin D 3 ; 2-methylene- 19-nor-24-homo- 1 ,25-dihydroxy-22-dehy drovitamin D3 ; 2-methylene- 19-nor-24-dihomo- 1 ,25-dihydroxy-22-dehydrovitamin D3 ; 2-methylene- 19-nor-24-trihomo- 1 ,25-dihydroxy-22-dehy drovitamin D3 ; 2-methylene- 19-nor-26,27-dimethyl-24
• vitamin D compounds of structure I when the side chain is saturated are: 2-methylene- 19-nor- 1 ⁇ -hydroxyvitamin D 3 ; 2-methylene- 19-nor- 1 ,25-dihydroxyvitamin D 3 ; 2-methylene- 19-nor- 1 ,24-dihydroxyvitamin D 3 ; 2-methylene- 19-nor-24-homo- 1 ,25-dihydroxyvitamin D3 ; 2-methylene- 19-nor-24-dihomo- 1 ,25-dihydroxyvitamin D3 ; 2-methylene- 19-nor-24-trihomo- 1 ,25-dihydroxyvitamin D3 ; 2-methylene- 19-nor-26,27-dimethyl-24-homo- 1 ,25-dihydroxyvitamin D3 ; 2-methylene- 19-nor-26,27-dimethyl-24-homo- 1 ,25-dihydroxyvitamin D3 ; 2-methylene- 19-nor-26,27-dimethyl-24-homo
• vitamin D compounds of structure I when the side chain is truncated are: 2-methylene- 19-nor- 1 ⁇ -hydroxy-pregnacalciferol; 2-methylene- 19-nor- 1 ⁇ -hydroxy-homopregnacalciferol; and 2-methylene- 19-nor- 1 ⁇ -hydroxy-bishomopregnacalciferol.
• the preferred compound is either l ⁇ -hydroxy-2- methylene- 19-nor-homopregnacalciferol (2-MP) having the following formula II :
• the most preferred compound is 2-MP.
• the preparation of vitamin D compounds having the basic structure I can be accomplished by a common general method, i.e. the condensation of a bicyclic Windaus-Grundmann type ketone IV with the allylic phosphine oxide V to the corresponding protected protected vitamin D analogs VI followed by deprotection, if desired, at C-1 and C-3 in the latter compounds to obtain the 2-methylene- 19-nor- vitamin D compounds of structure I:
• Y j , Y2 and R represent groups defined above; Yl and Y2 are preferably hydroxy-protecting groups, it being also understood that any functionalities in R that might be sensitive, or that interfere with the condensation reaction, be suitably protected as is well-known in the art.
• the process shown above represents an application of the convergent synthesis concept, which has been applied effectively for the preparation of vitamin D compounds [e.g. Lythgoe et al., J. Chem. Soc. Perkin Trans. I, 590 (1978); Lythgoe, Chem. Soc. Rev. 9, 449 (1983); Toh et al., J. Org. Chem.
• Hydrindanones of the general structure IV are known, or can be prepared by known methods. Specific important examples of such known bicyclic ketones are the structures with the side chains (a), (b), (c) and (d) described above, i.e. 25-hydroxy Grundmann's ketone (i) [Baggiolini et al., J. Org. Chem, 51, 3098 (1986)];
• vitamin D deficiency exacerbates symptoms of IBD in IL- 10 KO mice. Vitamin D deficiency also exacerbated the symptoms of enterocolitis in the animal model.
• EXAMPLE 1 Recently a number of transgenic animals have been developed in which IBD symptoms occur spontaneously.
• One of the best animal models for Crohn's disease is the IL-10 knockout (KO) mouse (Kuhn et al. 1993, Mac Donald 1994).
• the IL-10 KO mice develop enterocolitis within 5-8 weeks of life (Kuhn et al. 1993).
• Approximately 30% of the IL-10 KO mice die following the development of severe anemia and weight loss (Kuhn et al. 1993).
• the enterocolitis which develops in IL-10 KO mice is due to an uncontrolled immune response to conventional microflora since germfree IL-10 KO mice do not develop disease.
• mice raised in specific pathogen free facilities develop milder disease which doesn't result in the death of the mice (Kuhn et al. 1993).
• Vitamin D deficient IL-10 KO mice were randomly distributed into one of 4 groups. Controls remained vitamin D deficient for the entire 9-10 weeks of the experiment. Mice treated with l ⁇ ,25-dihydroxyvitamin D 3 (hereinafter "l,25(OH) 2 D 3 ”) were vitamin D deficient for 5 weeks and then received 5 ng/d in the diet for the remainder of the experiment. 2-MP treated mice were vitamin D deficient for 5 weeks and then received 720 ng/d in the diet for the remainder of the experiment. 2-MD treated mice were vitamin D deficient for 5 weeks and then received 0.12 ng/d in the diet for the remainder of the experiment. VDR KO IL10KO mice were bred with VDR KO mice and the double IL10/VDR KO mice are compared here.
• the active form of vitamin D and vitamin D analogs such as 2-MP and 2- MD inhibit the development of colitis in these IL-10 KO mice.
• the serum calcium values and the body weights of the mice on the vitamin D analogs are within the normal range for mice.
• the 2-MD analog was associated with a slight increase in serum calcium at this dose.
• the vitamin D analogs 2-MD and 2-MP are effective at inhibiting and/or preventing the development of colitis symptoms in this experimental model of IBD.
• 2-MP is preferred over 2-MD because it did not significantly change serum calcium yet clearly blocked the development of the disease.
• Standard treatments of patients with IBD include short-term high dose and long term low dose prednisone use (Andreassen et al. 1998, Podolosky 1991).
• Vitamin D supplementation of patients on corticosteroids has been shown to prevent steroid induced bone loss (Buckley et al. 1996).
• the hormonally active form of vitamin D (1,25-dihydroxycholecalciferol) is known to increase bone mineralization when given to experimental animals (Cantorna et al. 1998) and people (Ongphiphadhanakul et al. 2000).
• vitamin D and or 1,25-dihydroxycholecalciferol supplementation may be the maintenance of bone mineral density.
• 2-methylene- 19-nor-vitamin D compounds and especially 2-MP and 2-MD, are novel and effective treatments for IBD patients.
• a possible limitation of 2-MD treatment is the hypercalcemia which can result.
• the 2-MP analog is not calcemic and thus may be the treatment of choice because the danger of hypercalcemic is obviated.
• vitamin D analogs can be used in combination with prior standard treatments for IBD. The standard treatments often work well but have many side effects; like bone loss which some vitamin D analogs such as 2-MD could reverse or block entirely. Vitamin D analogs in combination with corticosteroids, or sulfasalazine drugs could reduce the effective dose of these drugs, limit side effects and prove to be novel and effective treatments for human IBD.
• novel compounds of this invention defined by formula I may be formulated for pharmaceutical applications as a solution in innocuous solvents, or as an emulsion, suspension or dispersion in suitable solvents or carriers, or as pills, tablets or capsules, together with solid carriers, according to ' conventional methods known in the art. Any such formulations may also contain other pharmaceutically-acceptable and non-toxic excipients such as stabilizers, anti-oxidants, binders, coloring agents or emulsifying or taste-modifying agents. The compounds may be administered orally, topically, parenterally or transdermally.
• the compounds are advantageously administered by injection or by intravenous infusion or suitable sterile solutions, or in the form of liquid or solid doses via the alimentary canal, or in the form of creams, ointments, patches, or similar vehicles suitable for transdermal applications.
• Doses of from 0.01 ⁇ g to lOmg per day per 160 pound person of the compounds are appropriate for treatment purposes, such doses being adjusted according to the activity of the particular compound being used, the disease to be treated, its severity and the response of the subject as is well understood in the art.
• Each compound may be suitably administered alone, or together with graded doses of another active vitamin D compound ⁇ e.g.
• compositions for use in the above-mentioned prevention and/or treatment of IBD comprise an effective amount of one or more vitamin D analog as defined by the above formula I as the active ingredient, and a suitable carrier.
• An effective amount of such compounds for use in accordance with this invention is from about 0.0 l ⁇ g to about lOO ⁇ g per gm of composition, and may be administered topically, transdermally, orally or parenterally in dosages of from about O.Ol ⁇ g/day to about lOmg/day per 160 pound person.
• the compounds may be formulated as creams, lotions, ointments, topical patches, pills, capsules or tablets, or in liquid form as solutions, emulsions, dispersions, or suspensions in pharmaceutically innocuous and acceptable solvent or oils, and such preparations may contain in addition other pharmaceutically innocuous or beneficial components, such as stabilizers, antioxidants, emulsifiers, coloring agents, binders or taste-modifying agents.
• the formulations of the present invention comprise an active ingredient in association with a pharmaceutically acceptable carrier therefore and optionally other therapeutic ingredients.
• the carrier must be "acceptable" in the sense of being compatible with the other ingredients of the formulations and not deleterious to the recipient thereof.
• Formulations of the present invention suitable for oral administration may be in the form of discrete units as capsules, sachets, tablets or lozenges, each containing a predetermined amount of the active ingredient; in the form of a powder or granules; in the form of a solution or a suspension in an aqueous liquid or non-aqueous liquid; or in the form of an oil-in-water emulsion or a water-in-oil emulsion.
• Formulations for rectal administration may be in the form of a suppository incorporating the active ingredient and carrier such as cocoa butter, or in the form of an enema.
• Formulations suitable for parenteral administration conveniently comprise a sterile oily or aqueous preparation of the active ingredient which is preferably isotonic with the blood of the recipient.
• Formulations suitable for topical administration include liquid or semi-liquid preparations such as liniments, lotions, applicants, oil-in-water or water-in-oil emulsions such as creams, ointments or pastes; or solutions or suspensions such as drops; or as sprays.
• the formulations may conveniently be presented in dosage unit form and may be prepared by any of the methods well known in the art of pharmacy.
• dosage unit is meant a unitary, i.e. a single dose which is capable of being administered to a patient as a physically and chemically stable unit dose comprising either the active ingredient as such or a mixture of it with solid or liquid pharmaceutical diluents or carriers.

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