WO2004069804A8 - Substrats enzymatiques, milieux de culture les contenant, utilisation pour detecter une activite aminopeptidase et/ou differencier des bacteries a gram positifs des bacteries a gram negatifs - Google Patents

Substrats enzymatiques, milieux de culture les contenant, utilisation pour detecter une activite aminopeptidase et/ou differencier des bacteries a gram positifs des bacteries a gram negatifs

Info

Publication number
WO2004069804A8
WO2004069804A8 PCT/FR2004/050031 FR2004050031W WO2004069804A8 WO 2004069804 A8 WO2004069804 A8 WO 2004069804A8 FR 2004050031 W FR2004050031 W FR 2004050031W WO 2004069804 A8 WO2004069804 A8 WO 2004069804A8
Authority
WO
WIPO (PCT)
Prior art keywords
gram
substrates
bacteria
culture media
media containing
Prior art date
Application number
PCT/FR2004/050031
Other languages
English (en)
Other versions
WO2004069804A1 (fr
Inventor
Arthur James
Annette Rigby
Original Assignee
Biomerieux Sa
Arthur James
Annette Rigby
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux Sa, Arthur James, Annette Rigby filed Critical Biomerieux Sa
Priority to US10/541,490 priority Critical patent/US7223865B2/en
Priority to BRPI0407072-0A priority patent/BRPI0407072B1/pt
Priority to JP2006502170A priority patent/JP4536714B2/ja
Priority to AU2004208895A priority patent/AU2004208895B2/en
Priority to DE602004010364T priority patent/DE602004010364T2/de
Priority to EP04705107A priority patent/EP1587793B1/fr
Publication of WO2004069804A1 publication Critical patent/WO2004069804A1/fr
Publication of WO2004069804A8 publication Critical patent/WO2004069804A8/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D219/00—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems
    • C07D219/02—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems with only hydrogen, hydrocarbon or substituted hydrocarbon radicals, directly attached to carbon atoms of the ring system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne de nouveaux substrats chromogéniques enzymatiques pour la détection chez des micro-organismes d'une activité aminopeptidase ou pour définir l'appartenance d'au moins une bactérie au groupe des Gram positifs ou des Gram négatifs selon sa coloration. L'invention concerne également des milieux de culture contenant de tels substrats, l'utilisation des substrats ou des milieux pour la détection d'activités aminopeptidases et/ou la différentiation de bactéries à Grain positif par rapport à des bactéries à Gram négatif et des procédés d'utilisation. Ces nouveaux substrats ont la formule suivante dans laquelle, R1 est rien ou un groupement alkyle, allyle, aryle, R2 est constitué par au moins un acide aminé, préférentiellement alanine, R3, R4, R5 et R6 sont constitués indépendamment l'un de l'autre par H- ou -O-alkyle, préférentiellement -O-CH3, et R7 est constitué par H, O-CH3, alkyle ou halogène, R8 est constitué par H ou Cl, et n est un chiffre entier correspondant à 0 ou 1. L'invention trouve une application préférentielle dans le domaine du diagnostic.
PCT/FR2004/050031 2003-01-29 2004-01-26 Substrats enzymatiques, milieux de culture les contenant, utilisation pour detecter une activite aminopeptidase et/ou differencier des bacteries a gram positifs des bacteries a gram negatifs WO2004069804A1 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
US10/541,490 US7223865B2 (en) 2003-01-29 2004-01-26 Chromogenic compounds, and use thereof as enzyme substrates
BRPI0407072-0A BRPI0407072B1 (pt) 2003-01-29 2004-01-26 Substratos enzimáticos, meios de cultura contendo os mesmos e aplicação dos mesmos de modo a detectar atividade de aminopeptidase e/ou diferenciar bactérias gram-positivas de bactérias gram-negativas
JP2006502170A JP4536714B2 (ja) 2003-01-29 2004-01-26 酵素基質、これを含む培養基、及び、アミノペプチダーゼ活性を検出するための、及び／又は、グラム陽性菌とグラム陰性菌とを識別するためのその使用
AU2004208895A AU2004208895B2 (en) 2003-01-29 2004-01-26 Enzyme substrates, culture media containing same and use thereof in order to detect aminopeptidase activity and/or differentiate gram-positive bacteria from gram-negative bacteria
DE602004010364T DE602004010364T2 (de) 2003-01-29 2004-01-26 Enzymsubstrate, diese enthaltende kulturmedien und deren verwendung zum nachweis von aminopeptidaseaktivität und/oder zur differenzierung von grampositiven bakterien von gramnegativenbakterien
EP04705107A EP1587793B1 (fr) 2003-01-29 2004-01-26 Substrats enzymatiques, milieux de culture les contenant, utilisation pour detecter une activite aminopeptidase et/ou differencier des bacteries à gram positifs des bacteries à gram negatifs

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FRFR0300953 2003-01-29
FR0300953A FR2850378B1 (fr) 2003-01-29 2003-01-29 Substrats enzymatiques, milieux de culture les contenant, utilisation pour detecter une activite aminopeptidase et/ou differencier des bacteries a gram+ par rapport a des bacteries a gram-

Publications (2)

Publication Number Publication Date
WO2004069804A1 WO2004069804A1 (fr) 2004-08-19
WO2004069804A8 true WO2004069804A8 (fr) 2005-10-27

Family

ID=32669291

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2004/050031 WO2004069804A1 (fr) 2003-01-29 2004-01-26 Substrats enzymatiques, milieux de culture les contenant, utilisation pour detecter une activite aminopeptidase et/ou differencier des bacteries a gram positifs des bacteries a gram negatifs

Country Status (10)

Country Link
US (1) US7223865B2 (fr)
EP (1) EP1587793B1 (fr)
JP (1) JP4536714B2 (fr)
CN (1) CN100343234C (fr)
AU (1) AU2004208895B2 (fr)
BR (1) BRPI0407072B1 (fr)
DE (1) DE602004010364T2 (fr)
ES (1) ES2297373T3 (fr)
FR (1) FR2850378B1 (fr)
WO (1) WO2004069804A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2875242B1 (fr) 2004-09-10 2006-11-17 Biomerieux Sa Nouveaux substrats enzymatiques derives de phenoxazinone et leur utilisation comme revelateur dans la detection de microorganismes a activite peptidase
FR2916763B1 (fr) * 2007-05-31 2013-11-15 Biomerieux Sa Nouveaux substrats de peptidase
FR2948684B1 (fr) * 2009-07-30 2011-08-19 Biomerieux Sa Nouveaux substrats de peptidase
FR2948685A1 (fr) * 2009-07-30 2011-02-04 Biomerieux Sa Nouveaux substrats
CN107090190B (zh) * 2017-04-24 2019-09-06 北京科技大学 一类吖啶盐染料及其制备方法和应用
CN108982430B (zh) * 2017-05-31 2020-09-29 北京大学 标记细菌菌群样品的试剂盒、方法及应用
CN113121439B (zh) * 2021-04-20 2022-05-03 山东大学 一种化合物、药物组合物、药物及其在制备抗菌产品中的应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2936543A1 (de) * 1979-09-10 1981-04-09 Behringwerke Ag, 3550 Marburg Chromogene verbindungen
DE3779066D1 (en) * 1986-12-09 1992-06-17 Miles Inc Acridinone als chromogene enzymsubstrate.
US4810636A (en) * 1986-12-09 1989-03-07 Miles Inc. Chromogenic acridinone enzyme substrates
FR2659982B1 (fr) * 1990-03-23 1995-10-20 Serbio Utilisation de substrats chromogenes dans la determination de candida.
FR2713240B1 (fr) 1993-12-02 1996-03-01 Bio Merieux Milieu nutritif pour la culture de microorganismes.
FR2751663B1 (fr) * 1996-07-29 1998-10-02 Bio Merieux Procede de mise en evidence d'une activite enzymatique de microorganismes
FR2767535B1 (fr) 1997-08-20 2000-02-04 Bio Merieux Milieux de culture et d'identification specifique de differentes especes de candida et procedes d'analyse
FR2774097B1 (fr) * 1998-01-28 2000-09-29 Bio Merieux Utilisation de derives d'indolamine dans la detection d'une activite de peptidase ou dans la detection de micro-organismes
DE60018758T2 (de) 1999-07-30 2006-01-19 Bayer Corp. Chemiliumineszierendes Substrat von Hydrolasen wie Phosphatasen

Also Published As

Publication number Publication date
AU2004208895B2 (en) 2009-10-01
BRPI0407072B1 (pt) 2014-02-18
FR2850378A1 (fr) 2004-07-30
JP2006516401A (ja) 2006-07-06
EP1587793B1 (fr) 2007-11-28
AU2004208895A1 (en) 2004-08-19
CN1745066A (zh) 2006-03-08
ES2297373T3 (es) 2008-05-01
EP1587793A1 (fr) 2005-10-26
FR2850378B1 (fr) 2005-03-04
CN100343234C (zh) 2007-10-17
US20060035306A1 (en) 2006-02-16
WO2004069804A1 (fr) 2004-08-19
DE602004010364D1 (de) 2008-01-10
DE602004010364T2 (de) 2008-06-26
BRPI0407072A (pt) 2006-01-24
US7223865B2 (en) 2007-05-29
JP4536714B2 (ja) 2010-09-01

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