BRPI0407072A - Substratos enzimáticos, meios de cultura contendo os mesmos e aplicação dos mesmos de modo a detectar atividade de aminopeptidase e/ou diferenciar bactérias gram-positivas de bactérias gram-negativas - Google Patents

Substratos enzimáticos, meios de cultura contendo os mesmos e aplicação dos mesmos de modo a detectar atividade de aminopeptidase e/ou diferenciar bactérias gram-positivas de bactérias gram-negativas

Info

Publication number
BRPI0407072A
BRPI0407072A BR0407072-0A BRPI0407072A BRPI0407072A BR PI0407072 A BRPI0407072 A BR PI0407072A BR PI0407072 A BRPI0407072 A BR PI0407072A BR PI0407072 A BRPI0407072 A BR PI0407072A
Authority
BR
Brazil
Prior art keywords
gram
substrates
positive
application
aminopeptidase activity
Prior art date
Application number
BR0407072-0A
Other languages
English (en)
Inventor
Arthur James
Annette Rigby
Original Assignee
Biomerieux Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux Sa filed Critical Biomerieux Sa
Publication of BRPI0407072A publication Critical patent/BRPI0407072A/pt
Publication of BRPI0407072B1 publication Critical patent/BRPI0407072B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D219/00—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems
    • C07D219/02—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems with only hydrogen, hydrocarbon or substituted hydrocarbon radicals, directly attached to carbon atoms of the ring system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"SUBSTRATOS ENZIMáTICOS, MEIOS DE CULTURA CONTENDO OS MESMOS E APLICAçãO DOS MESMOS DE MODO A DETECTAR ATIVIDADE DE AMINOPEPTIDASE E/OU DIFERENCIAR BACTéRIAS GRAM-POSITIVAS DE BACTéRIAS GRAM-NEGATIVAS". A presente invenção refere-se a novos substratos cromogênicos os quais são usados para detectar atividade de aminopeptidase em microorganismos ou para determinar se no mínimo uma bactéria pertence ao grupo Gram-positivo ou ao grupo Gram-negativo de acordo com a cor da mesma. A invenção refere-se também a meios de cultura contendo os substratos referidos, à aplicação dos substratos ou meios para a detecção de atividades de aminopeptidase e/ou a diferenciação de bactérias Gram-positivas de bactérias Gram-negativas e a métodos de aplicação. Os novos substratos mencionados acima têm a fórmula abaixo: na qual: * R~ 1~ é nada ou um grupo alquíla, alila ou arila, * R~ 2~ consiste em no mínimo um aminoácido, preferencialmente alanina, e R~ 3~, R~ 4~, R~ 5~ e R~ 6~ consistem, de modo independente um do outro, em H- ou -O-alquila, preferencialmente -0-CH3, R~ 7~ consiste em H, 0-CH3, alquila ou halogênio, R~ 8~ consiste em H ou Cl, e n é um inteiro correspondente a O ou 1. A invenção é particularmente adequada para aplicação no campo de diagnóstico.
BRPI0407072-0A 2003-01-29 2004-01-26 Substratos enzimáticos, meios de cultura contendo os mesmos e aplicação dos mesmos de modo a detectar atividade de aminopeptidase e/ou diferenciar bactérias gram-positivas de bactérias gram-negativas BRPI0407072B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0300953A FR2850378B1 (fr) 2003-01-29 2003-01-29 Substrats enzymatiques, milieux de culture les contenant, utilisation pour detecter une activite aminopeptidase et/ou differencier des bacteries a gram+ par rapport a des bacteries a gram-
FR0300953 2003-01-29
PCT/FR2004/050031 WO2004069804A1 (fr) 2003-01-29 2004-01-26 Substrats enzymatiques, milieux de culture les contenant, utilisation pour detecter une activite aminopeptidase et/ou differencier des bacteries a gram positifs des bacteries a gram negatifs

Publications (2)

Publication Number Publication Date
BRPI0407072A true BRPI0407072A (pt) 2006-01-24
BRPI0407072B1 BRPI0407072B1 (pt) 2014-02-18

Family

ID=32669291

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0407072-0A BRPI0407072B1 (pt) 2003-01-29 2004-01-26 Substratos enzimáticos, meios de cultura contendo os mesmos e aplicação dos mesmos de modo a detectar atividade de aminopeptidase e/ou diferenciar bactérias gram-positivas de bactérias gram-negativas

Country Status (10)

Country Link
US (1) US7223865B2 (pt)
EP (1) EP1587793B1 (pt)
JP (1) JP4536714B2 (pt)
CN (1) CN100343234C (pt)
AU (1) AU2004208895B2 (pt)
BR (1) BRPI0407072B1 (pt)
DE (1) DE602004010364T2 (pt)
ES (1) ES2297373T3 (pt)
FR (1) FR2850378B1 (pt)
WO (1) WO2004069804A1 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2875242B1 (fr) * 2004-09-10 2006-11-17 Biomerieux Sa Nouveaux substrats enzymatiques derives de phenoxazinone et leur utilisation comme revelateur dans la detection de microorganismes a activite peptidase
FR2916763B1 (fr) * 2007-05-31 2013-11-15 Biomerieux Sa Nouveaux substrats de peptidase
FR2948684B1 (fr) * 2009-07-30 2011-08-19 Biomerieux Sa Nouveaux substrats de peptidase
FR2948685A1 (fr) * 2009-07-30 2011-02-04 Biomerieux Sa Nouveaux substrats
CN107090190B (zh) * 2017-04-24 2019-09-06 北京科技大学 一类吖啶盐染料及其制备方法和应用
CN108982430B (zh) * 2017-05-31 2020-09-29 北京大学 标记细菌菌群样品的试剂盒、方法及应用
CN113121439B (zh) * 2021-04-20 2022-05-03 山东大学 一种化合物、药物组合物、药物及其在制备抗菌产品中的应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2936543A1 (de) * 1979-09-10 1981-04-09 Behringwerke Ag, 3550 Marburg Chromogene verbindungen
ATE142202T1 (de) * 1986-12-09 1996-09-15 Bayer Ag Acridinone als chromogene enzymsubstrate
US4810636A (en) * 1986-12-09 1989-03-07 Miles Inc. Chromogenic acridinone enzyme substrates
FR2659982B1 (fr) * 1990-03-23 1995-10-20 Serbio Utilisation de substrats chromogenes dans la determination de candida.
FR2713240B1 (fr) 1993-12-02 1996-03-01 Bio Merieux Milieu nutritif pour la culture de microorganismes.
FR2751663B1 (fr) * 1996-07-29 1998-10-02 Bio Merieux Procede de mise en evidence d'une activite enzymatique de microorganismes
FR2767535B1 (fr) 1997-08-20 2000-02-04 Bio Merieux Milieux de culture et d'identification specifique de differentes especes de candida et procedes d'analyse
FR2774097B1 (fr) * 1998-01-28 2000-09-29 Bio Merieux Utilisation de derives d'indolamine dans la detection d'une activite de peptidase ou dans la detection de micro-organismes
JP3819294B2 (ja) 1999-07-30 2006-09-06 バイエル コーポレイション 燐酸酵素のような加水分解性酵素の化学発光基質

Also Published As

Publication number Publication date
CN100343234C (zh) 2007-10-17
EP1587793B1 (fr) 2007-11-28
ES2297373T3 (es) 2008-05-01
BRPI0407072B1 (pt) 2014-02-18
AU2004208895A1 (en) 2004-08-19
DE602004010364T2 (de) 2008-06-26
JP2006516401A (ja) 2006-07-06
EP1587793A1 (fr) 2005-10-26
AU2004208895B2 (en) 2009-10-01
FR2850378A1 (fr) 2004-07-30
FR2850378B1 (fr) 2005-03-04
WO2004069804A8 (fr) 2005-10-27
US20060035306A1 (en) 2006-02-16
JP4536714B2 (ja) 2010-09-01
DE602004010364D1 (de) 2008-01-10
WO2004069804A1 (fr) 2004-08-19
US7223865B2 (en) 2007-05-29
CN1745066A (zh) 2006-03-08

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 18/02/2014, OBSERVADAS AS CONDICOES LEGAIS.