WO2004054375A2 - Agents et procede de traitement de produits frais - Google Patents

Agents et procede de traitement de produits frais Download PDF

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Publication number
WO2004054375A2
WO2004054375A2 PCT/ZA2003/000184 ZA0300184W WO2004054375A2 WO 2004054375 A2 WO2004054375 A2 WO 2004054375A2 ZA 0300184 W ZA0300184 W ZA 0300184W WO 2004054375 A2 WO2004054375 A2 WO 2004054375A2
Authority
WO
WIPO (PCT)
Prior art keywords
family
fungus
bacterium
yeast
fresh produce
Prior art date
Application number
PCT/ZA2003/000184
Other languages
English (en)
Other versions
WO2004054375A3 (fr
Inventor
Helga Dagutat
Original Assignee
Dagutat Biolab Cc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dagutat Biolab Cc filed Critical Dagutat Biolab Cc
Priority to AU2003297543A priority Critical patent/AU2003297543A1/en
Publication of WO2004054375A2 publication Critical patent/WO2004054375A2/fr
Publication of WO2004054375A3 publication Critical patent/WO2004054375A3/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3571—Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/32—Yeast
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/38—Trichoderma
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00—Preservation or chemical ripening of fruit or vegetables
    • A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
    • A23B7/154—Organic compounds; Microorganisms; Enzymes
    • A23B7/155—Microorganisms; Enzymes; Antibiotics

Definitions

  • This invention relates to means and method for treating fresh produce. More particularly, this invention relates to a biological means and method for treating fresh produce i.e fruit and vegetables.
  • Such deterioration or spoilage includes breakdown at the cellular level of such produce and also includes diseases such as fungal and/or bacterial attack.
  • certain health hazards can also arise during post- harvest spoilage of fresh produce for example certain fungal species of Botrytis, Penicillium and Aspergillus can grow on fruit, including apples, pears, grapes and other fruit and also on vegetables. These fungi, as well as fungi that can grow on ground nuts for example range in toxicity from being mildly toxic to being highly toxic.
  • Another method of slowing the rate of breakdown is by using sulphur dioxide (either SO 2 by fumigation or through SO 2 generating pads) a few hours after harvesting, and prior to transportation of produce by sea. This has been employed in the packaging and exporting of grapes, for example.
  • sulphur dioxide either SO 2 by fumigation or through SO 2 generating pads
  • a means for post- harvest treatment of fresh produce including a suitable concentration of at least one fungus selected from the Order: Hypocreales; and/or at least one yeast selected from the Order: Saccharomycetales and/or the Order: Filobasidiales; and/or at least one bacterium selected from the family Pseudomonadaceae, excluding Pseudomonas fluorescens, and/or the family Bacillaceae, excluding Bacillus subtils, and/or the family Lactobacilleae and/or the family Microbacteriaceae.
  • the fungus may be selected from Trichoderma sp., and may more specifically be Trichoderma harzianum.
  • the yeast may be selected from Cryptococcus sp,, and may more specifically be Cryptococcus albidus.
  • the bacterium may be selected from Microbacterium sp., and may more specifically be Microbacterium maritypicum (Basonym: Flavobacterium marinotypicum).
  • the fungus, yeast and/or bacterium may be applied in a suitable concentration or dosage on a suitable substrate or carrier.
  • the substrate or carrier may be a sheet of cellulose material including paper, card, cardboard, wood, or the like.
  • the fungus, yeast and/or bacterium may be provided in a slow release form, for example, in a suitable wax medium.
  • the fungus, yeast and/or bacterium may be selected to withstand low temperatures and may be able to control pathogens when fresh produce is stored at such low temperatures, for example from about 0.5 degrees Centigrade to about 5.0 degrees Centigrade.
  • a means of post-harvest treatment of fresh produce including an active concentration of any suitable organism whenever applied to a sheet of cellulose material including paper, card, cardboard, wood, or the like.
  • a method for post-harvest treatment of fresh produce including the steps of using a suitable concentration of at least one fungus selected from the Order: Hypocreales; and/or at least one yeast selected from the Order: Saccharomycetales and/or the
  • Filobasidiales and/or at least one bacterium selected from the family Pseudomonadaceae, excluding Pseudomonas fluorescens, and/or the family Bacillaceae, excluding Bacillus subtilis, and/or the family Lactobacilleae and/or the family Microbacteriaceae, and providing such in close proximity to fresh produce.
  • the method may include the step of applying the fungus, yeast and/or bacterium in a sufficient concentration to a suitable substrate or carrier including paper, card, cardboard, wood, or the like.
  • the method may include the step of providing the fungus, yeast and/or bacterium in a slow release form.
  • the method may include the step of subjecting the fungus, yeast and/or bacterium and fresh produce to low temperatures, for example, from about 0.5 degrees Centigrade to about 5.0 degrees Centigrade.
  • the aforementioned fungus, yeast and/or bacteria may conveniently be applied in a sufficient dosage on a suitable substrate or carrier, whether made from a natural or synthetic substance, such as suitable sheets of cellulose material, for example paper.
  • the substrate or carrier may be part of the inside of a container in which fruit or vegetables are packed.
  • Such sheets and containers provide not only a medium which retains a certain amount of moisture but also a medium on which the fungus, yeast and/or bacteria may feed and survive.
  • the fungus, yeast and/or bacteria must withstand low temperatures and be able to control pathogens when fruit and vegetables are stored at low temperatures.
  • a solution or suspension of the fungus may be sprayed onto sheets of paper or on the inside of the container itself. Such sprayed sheets may then be provided in a container for example below and above a layer of produce in such container which may itself be treated on the inside thereof.
  • a method was used to prepare a microbial inoculant including the step of cultivating a desired microbe more particularly the fungus Trichoderma harzianum in a suitable growth medium in a container until an optimal cell density was attained. Once this cell density had been attained, the inoculant can be kept viable in various forms for example (a) as a liquid inoculant; (b) by freeze-drying to provide a powder form; or (c) by adding suitable carriers to the microbes.
  • the fungus inoculant could be dissolved and/or brought into suspension by using any suitable solvent/semi-solvent, for example water at the correct pH, for purposes of applying the fungus to a substrate.
  • the powder inoculant or liquid inoculant containing the micro-organism was diluted, dissolved or suspended in sufficient water so that the concentration thereof as applied to paper was effective for optimal biological control of plant pathogens.
  • Trichoderma harzianum For example 1 x 10 9 active colony forming units/gram powder Trichoderma harzianum was used as an active ingredient and 450g inoculant thereof in powder form was suspended in 20 to 50 litre water.
  • the pH range of the water is adjusted according to the requirements of the specific organism used. In this case the pH was adjusted in a range of from about 6.0 to about 7.0.
  • Any suitable buffer that will not deleteriously affect the organism(s) may be used to reach and stabilize the pH range.
  • acetic acid was used as a buffer to decrease the pH of the water.
  • Lime may be used to increase the pH of the water.
  • the mixture/suspension of water and fungus inoculant is applied to suitable paper sheets, for example 60g paper sheets although kraft paper or newsprint may also be used, by means of spraying, dusting or rolling.
  • spraying was used to ensure that sufficient viable micro-organism is applied to the paper per cm 2 to assure anti-pathogen activity.
  • the micro-organism may also be sprayed or otherwise applied to the interior surfaces of fresh produce containers.
  • paper sheets/container material do not contain any harmful chemicals or substances to harm the micro-organism. It is also important that the paper sheets/container material are such as to keep the micro-organism viable for biological control of pathogenic organisms.
  • treated sheets/container material were then examined to ensure the fungus inoculant was alive and viable.
  • Such sheets/container panels were stored for several weeks and subsequent examination revealed that the fungus had remained alive and viable.
  • treated sheets/containers may be dispatched to remote venues for example in other countries to be used for treating fresh produce post- harvesting.
  • the treated sheets (from EXAMPLE 2) were placed inside a fruit container (for example a citrus container) namely on the floor thereof in effect to provide a lower lining. Citrus was packed on top of the treated sheets and further sheets were placed vertically against the inside surface of the container in effect to provide four side linings. Alternatively, the inside surfaces of the container itself may be treated. Thereafter another layer of treated sheets was placed on top of the citrus when the container was filled, in effect to provide an upper lining.
  • a fruit container for example a citrus container
  • Citrus was packed on top of the treated sheets and further sheets were placed vertically against the inside surface of the container in effect to provide four side linings. Alternatively, the inside surfaces of the container itself may be treated. Thereafter another layer of treated sheets was placed on top of the citrus when the container was filled, in effect to provide an upper lining.
  • EXAMPLE 4 Use of treated sheets and containers for grapes.
  • the treated sheets (from EXAMPLE 2) were placed inside a fruit container (for example a grape container) namely on the floor thereof in effect to provide a lower lining.
  • Bunches of grapes provided in polyethylene bags having slits therein were
  • the container itself may be treated on the inside surfaces thereof.
  • the aforementioned provides a simple and effective means and method for keeping produce fresh, for example during transportation to international destinations. More particularly it will be understood that refrigerated shipping can be used for such transportation instead of costly airfreight.
  • a suitable means and method for prolonging the effective shelf-life of fresh produce in a relatively simple, inexpensive and safe manner.
  • the means and method are effective and generally exceed the results obtained from for example sulphur dioxide sheets and avoid the undesirable (and increasingly unacceptable) use of sulphur dioxide as a preservative substance.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

L'invention concerne des agents de traitement après récolte de produits frais, ces agents comprenant une concentration appropriée d'au moins un champignon choisi dans l'ordre Hypocreales, et/ou d'au moins une levure choisie dans l'ordre Saccharomycetales et/ou l'ordre Filobasidiales, et/ou d'au moins une bactérie choisie dans la famille Pseudomonadaceae, à l'exception de Pseudomonas fluorescens, et/ou la famille Bacillaceae, à l'exception de Bacillus subtilis, et/ou la famille Lactobacilleae et/ou la famille Microbacteriaceae. Ce champignon, cette levure et/ou cette bactérie peuvent être appliqués en concentration ou en dose appropriée sur un substrat ou un support approprié tel qu'une matière cellulosique sous forme de papier, de papier-carton, de carton, de bois ou analogue. Le champignon, la levure et/ou la bactérie peuvent être sélectionnés pour leur résistance aux basses températures et permettent de lutter contre des agents pathogènes lorsque les produits frais sont stockés à basses températures, par exemple, entre environ 0,5 °C et environ 5,0 °C. L'invention concerne également un procédé de traitement après récolte de produits frais, ce procédé consistant à utiliser une concentration appropriée d'au moins un champignon choisi dans l'ordre Hypocreales, et/ou d'au moins une levure choisie dans l'ordre Saccharomycetales et/ou l'ordre Filobasidiales, et/ou d'au moins une bactérie choisie dans la famille Pseudomonadaceae, à l'exception de Pseudomonas fluorescens, et/ou la famille Bacillaceae, à l'exception de Bacillus subtilis, et/ou la famille Lactobacilleae et/ou la famille Microbacteriaceae, et à les appliquer au voisinage immédiat des produits frais.
PCT/ZA2003/000184 2002-12-18 2003-12-17 Agents et procede de traitement de produits frais WO2004054375A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003297543A AU2003297543A1 (en) 2002-12-18 2003-12-17 Means and method for treating fresh produce

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ZA2002/10228 2002-12-18
ZA200210228 2002-12-18

Publications (2)

Publication Number Publication Date
WO2004054375A2 true WO2004054375A2 (fr) 2004-07-01
WO2004054375A3 WO2004054375A3 (fr) 2004-11-11

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ID=32597743

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Application Number Title Priority Date Filing Date
PCT/ZA2003/000184 WO2004054375A2 (fr) 2002-12-18 2003-12-17 Agents et procede de traitement de produits frais

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AU (1) AU2003297543A1 (fr)
WO (1) WO2004054375A2 (fr)
ZA (1) ZA200508428B (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102210343A (zh) * 2010-04-02 2011-10-12 东北农业大学 一种用于萝卜采摘后保鲜的浅白隐球酵母菌

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1573850A (en) * 1976-09-13 1980-08-28 Richard J Mycofungicidal products
DE3600394A1 (de) * 1986-01-09 1987-07-23 Wolf Louis Schadpilzbekaempfung mit pilzen der gattung trichoderma
US4748021A (en) * 1983-07-28 1988-05-31 Yissum Research And Development Company Of The Hebrew University Of Jerusalem Antifungal compositions containing trichoderma active against fusarium
US4828600A (en) * 1987-07-29 1989-05-09 Cetus Corporation Biological inoculant for corn
US5047239A (en) * 1984-05-01 1991-09-10 The United States Of America As Represented By The Secretary Of Agriculture Biological control of fruit rot
EP0466133A2 (fr) * 1990-07-12 1992-01-15 Peri Development Applications (1985) Ltd. Souche de trichoderma harzianum, T-39, compositions fongicides les contenant et utilisation contre B. cinerea et S. sclerotiorum

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1124473A1 (ru) * 1982-12-22 1991-03-30 МГУ им.М.В.Ломоносова Штамм гриба ТRIсноDеRма наRZIаNUм RIFaI N ВКМ F-2477, используемый дл защиты растений огурца от аскохитоза
CN1337164A (zh) * 2000-08-09 2002-02-27 玉溪红塔烟草(集团)有限责任公司 一种微生物杀菌剂及其制备方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1573850A (en) * 1976-09-13 1980-08-28 Richard J Mycofungicidal products
US4748021A (en) * 1983-07-28 1988-05-31 Yissum Research And Development Company Of The Hebrew University Of Jerusalem Antifungal compositions containing trichoderma active against fusarium
US5047239A (en) * 1984-05-01 1991-09-10 The United States Of America As Represented By The Secretary Of Agriculture Biological control of fruit rot
DE3600394A1 (de) * 1986-01-09 1987-07-23 Wolf Louis Schadpilzbekaempfung mit pilzen der gattung trichoderma
US4828600A (en) * 1987-07-29 1989-05-09 Cetus Corporation Biological inoculant for corn
EP0466133A2 (fr) * 1990-07-12 1992-01-15 Peri Development Applications (1985) Ltd. Souche de trichoderma harzianum, T-39, compositions fongicides les contenant et utilisation contre B. cinerea et S. sclerotiorum

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch, Week 199129 Derwent Publications Ltd., London, GB; Class C03, AN 1991-213689 XP002288503 -& SU 1 124 473 A (MOSCOW LOMONOSOV UNIV) 30 March 1991 (1991-03-30) *
DATABASE WPI Section Ch, Week 200239 Derwent Publications Ltd., London, GB; Class C05, AN 2002-353101 XP002288504 & CN 1 337 164 A (YUXI HONGTA TOBACCO GROUP CORP LTD) 27 February 2002 (2002-02-27) *
MCGUIRE R.: "compositions of cellulose coatings affect populations of yeasts in the liquid formulation and on coated grapefruits" PROCEEDINGS OF THE FLORIDA STATE HORTICULTURE SOCIETY, vol. 107, 1994, pages 293-298, XP008032775 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102210343A (zh) * 2010-04-02 2011-10-12 东北农业大学 一种用于萝卜采摘后保鲜的浅白隐球酵母菌

Also Published As

Publication number Publication date
AU2003297543A1 (en) 2004-07-09
AU2003297543A8 (en) 2004-07-09
WO2004054375A3 (fr) 2004-11-11
ZA200508428B (en) 2007-12-27

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