WO2004029256A3 - Vector para la producción de plantas angiospermas transplastómicas - Google Patents

Vector para la producción de plantas angiospermas transplastómicas Download PDF

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Publication number
WO2004029256A3
WO2004029256A3 PCT/CU2003/000009 CU0300009W WO2004029256A3 WO 2004029256 A3 WO2004029256 A3 WO 2004029256A3 CU 0300009 W CU0300009 W CU 0300009W WO 2004029256 A3 WO2004029256 A3 WO 2004029256A3
Authority
WO
WIPO (PCT)
Prior art keywords
vector
atpb
rbcl
transplastomic
production
Prior art date
Application number
PCT/CU2003/000009
Other languages
English (en)
French (fr)
Other versions
WO2004029256A2 (es
Inventor
Sosa Guillermo Selman-Housein
Cabeza Eduardo Aguiar
Quintero Annery Del C Gonzalez
Gonzalez Osmany Ramos
Original Assignee
Ct Ingenieria Genetica Biotech
Sosa Guillermo Selman-Housein
Cabeza Eduardo Aguiar
Quintero Annery Del C Gonzalez
Gonzalez Osmany Ramos
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct Ingenieria Genetica Biotech, Sosa Guillermo Selman-Housein, Cabeza Eduardo Aguiar, Quintero Annery Del C Gonzalez, Gonzalez Osmany Ramos filed Critical Ct Ingenieria Genetica Biotech
Priority to JP2004538665A priority Critical patent/JP2006500037A/ja
Priority to EP03798037A priority patent/EP1557468A2/en
Priority to BR0314841-6A priority patent/BR0314841A/pt
Priority to AU2003266096A priority patent/AU2003266096A1/en
Priority to CA002496913A priority patent/CA2496913A1/en
Priority to US10/527,048 priority patent/US20060026704A1/en
Priority to MXPA05003185A priority patent/MXPA05003185A/es
Publication of WO2004029256A2 publication Critical patent/WO2004029256A2/es
Publication of WO2004029256A3 publication Critical patent/WO2004029256A3/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)

Abstract

Un vector de ADN que permite la inserción estable y la expresión de genes heterólogos en los genomas de plastidios de células de cualquier planta Angiosperma.. En este vector los genes a introducir se pueden insertar en múltiples sitios de clonación localizados en una región intergénica artificial que se obtiene de combinar los operones atpB y rbcL pertenecientes a plantas de clases diferentes (dicotiledóneas o monocotiledóneas). El casete de expresión se integra al genoma mediante recombinación entre las secuencias bordes atpB y rbcL del vector, con las correspondientes regiones homólogas del genoma plastídico; pudiendo expresarse más de un gen de interés a partir del promotor rbcL adyacente a la región borde que codifica para el gen atpB
PCT/CU2003/000009 2002-09-27 2003-09-15 Vector para la producción de plantas angiospermas transplastómicas WO2004029256A2 (es)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2004538665A JP2006500037A (ja) 2002-09-27 2003-09-15 被子植物遺伝子導入植物を作製するためのベクター
EP03798037A EP1557468A2 (en) 2002-09-27 2003-09-15 Vector for the production of transplastomic angiosperm plants
BR0314841-6A BR0314841A (pt) 2002-09-27 2003-09-15 Vetor de dna, cultura, plantas angiospérmicas transplastÈmicas, progênie das mesmas e purificação e utilização da proteìna ou proteìnas que produzem as células das plantas
AU2003266096A AU2003266096A1 (en) 2002-09-27 2003-09-15 Vector for the production of transplastomic angiosperm plants
CA002496913A CA2496913A1 (en) 2002-09-27 2003-09-15 Vector for the production of transplastomic angiosperm plants
US10/527,048 US20060026704A1 (en) 2002-09-27 2003-09-15 Vector for the production of transplastomic angiosperm plants
MXPA05003185A MXPA05003185A (es) 2002-09-27 2003-09-15 Vector para la produccion de plantas angiospermas transplastomicas.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CU2002-0208 2002-09-27
CU20020208 2002-09-27

Publications (2)

Publication Number Publication Date
WO2004029256A2 WO2004029256A2 (es) 2004-04-08
WO2004029256A3 true WO2004029256A3 (es) 2004-04-29

Family

ID=32034750

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CU2003/000009 WO2004029256A2 (es) 2002-09-27 2003-09-15 Vector para la producción de plantas angiospermas transplastómicas

Country Status (12)

Country Link
US (1) US20060026704A1 (es)
EP (1) EP1557468A2 (es)
JP (1) JP2006500037A (es)
KR (1) KR20050054928A (es)
CN (1) CN1685047A (es)
AR (1) AR041375A1 (es)
AU (1) AU2003266096A1 (es)
BR (1) BR0314841A (es)
CA (1) CA2496913A1 (es)
MX (1) MXPA05003185A (es)
WO (1) WO2004029256A2 (es)
ZA (1) ZA200501740B (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2598792A1 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
US20140199313A1 (en) 2005-03-02 2014-07-17 Metanomics Gmbh Process for the Production of Fine Chemicals
JP2006246744A (ja) * 2005-03-09 2006-09-21 Research Organization Of Information & Systems 遺伝子導入方法、ジーンターゲッティング方法及びトランスジェニック植物の製造方法
KR100740694B1 (ko) * 2006-02-09 2007-07-18 한국생명공학연구원 색소체 게놈 내에 삽입된 외래유전자의 이차 재조합을방지하기 위한 벡터개발
GB0604938D0 (en) 2006-03-11 2006-04-19 Renovo Ltd Proteins, nucleic acids and medicaments
GB0604966D0 (en) 2006-03-11 2006-04-19 Renovo Ltd Medicaments and proteins
GB0604964D0 (en) 2006-03-11 2006-04-19 Renovo Ltd Protein folding
GB0617816D0 (en) * 2006-09-11 2006-10-18 Renovo Ltd Nucleic acids and methods of protein expression
US8044260B2 (en) * 2007-03-09 2011-10-25 Monsanto Technology Llc Method of meristem excision and transformation
US20110037083A1 (en) * 2009-01-14 2011-02-17 Alex Chi Keung Chan Led package with contrasting face
CN102618566B (zh) * 2011-01-31 2014-01-08 中国科学院海洋研究所 一种构建亚心型扁藻叶绿体表达系统的方法
CN108192897A (zh) * 2018-02-11 2018-06-22 云南省烟草农业科学研究院 一种烟草rbcl基因片段及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0251654A2 (en) * 1986-06-23 1988-01-07 Biotechnica International, Inc. Transformation of chloroplasts
WO1997006250A1 (en) * 1995-08-10 1997-02-20 Rutgers University Nuclear-encoded transcription system in plastids of higher plants
WO1998055595A1 (en) * 1997-06-03 1998-12-10 Rutgers, The State University Of New Jersey Plastid promoters for transgene expression in the plastids of higher plants

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0251654A2 (en) * 1986-06-23 1988-01-07 Biotechnica International, Inc. Transformation of chloroplasts
WO1997006250A1 (en) * 1995-08-10 1997-02-20 Rutgers University Nuclear-encoded transcription system in plastids of higher plants
WO1998055595A1 (en) * 1997-06-03 1998-12-10 Rutgers, The State University Of New Jersey Plastid promoters for transgene expression in the plastids of higher plants

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BLOWERS A ET AL: "Transcriptional analysis of endogenous and foreign genes in chloroplast transformants of chlamydomonas", THE PLANT CELL, vol. 2, no. 11, November 1990 (1990-11-01), pages 1059 - 1070, XP002902992 *
KLEIN U ET AL: "Two types of chloroplast gene promoters in chlamydomonas reinhardtii", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES USA, vol. 89, no. 8, 15 April 1992 (1992-04-15), pages 3453 - 3457, XP002902993 *
ROGERS S A ET AL: "Analysis of chloroplast promoters using bidirectional transcription vectors.", PLANT MOLECULAR BIOLOGY, vol. 15, no. 3, 1990, pages 421 - 435, XP002902994 *

Also Published As

Publication number Publication date
KR20050054928A (ko) 2005-06-10
US20060026704A1 (en) 2006-02-02
CA2496913A1 (en) 2004-04-08
ZA200501740B (en) 2005-09-06
CN1685047A (zh) 2005-10-19
EP1557468A2 (en) 2005-07-27
AR041375A1 (es) 2005-05-11
JP2006500037A (ja) 2006-01-05
MXPA05003185A (es) 2005-06-08
AU2003266096A1 (en) 2004-04-19
WO2004029256A2 (es) 2004-04-08
BR0314841A (pt) 2005-08-09

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