AR041375A1 - Vector para la produccion de plantas angiospermas transplastomicas - Google Patents

Vector para la produccion de plantas angiospermas transplastomicas

Info

Publication number
AR041375A1
AR041375A1 ARP030103474A ARP030103474A AR041375A1 AR 041375 A1 AR041375 A1 AR 041375A1 AR P030103474 A ARP030103474 A AR P030103474A AR P030103474 A ARP030103474 A AR P030103474A AR 041375 A1 AR041375 A1 AR 041375A1
Authority
AR
Argentina
Prior art keywords
plants
genes
vector
gene
transplastomic
Prior art date
Application number
ARP030103474A
Other languages
English (en)
Inventor
Sosa Manuel Selman-Housein
Cabeza Eduardo Aguiar
Quintero Annery Del Carmen Gonzalez
Gonzalez Osmany Ramos
Original Assignee
Ct Ingenieria Genetica Biotech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct Ingenieria Genetica Biotech filed Critical Ct Ingenieria Genetica Biotech
Publication of AR041375A1 publication Critical patent/AR041375A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un vector de ADN que permite la inserción estable y la expresión de genes heterólogos en los genomas de plastidios de células de cualquier planta Angiosperma.. En este vector los genes a introducir se pueden insertar en múltiples sitios de clonaciónlocalizados en una región intergénica artificial que se obtiene de combinar los operones atpB y rbcL pertenecientes a plantas de clases diferentes (dicotiledóneas o monocotiledóneas). El casete de expresión se integra al genoma medianterecombinación entre las secuencias bordes atpB y rbcL del vector, con las correspondientes regiones homólogas del genoma plastídico; pudiendo expresarse más de un gen de interés a partir del promotor rbcL adyacente a la región borde que codificapara el gen atpB. Reivindicación 1: Un vector de ADN útil para la transformación estable y expresión de genes en plastidios, donde el gen a expresar se introduce en una región intergénica artificial formada por la combinación de dos regiones 5' notraducibles de genes que se transcriben en direcciones divergentes y pertenecientes a plantas de divisiones o clases diferentes. Reivindicación 116: El cultivo de las células vegetales en presencia de citoquininas, antes de introducirles cualquierade los vectores de ADN enunciados en las reivindicaciones de al 1 a la 115, para aumentar la frecuencia de producción de plantas transplastómicas. Reivindicación 117: El empleo de kinetina según la reivindicación 116. Reivindicación 118: Lasplantas Angiospermas transplastómicas establemente transformadas con cualquiera de los vectores de ADN enunciados en las reivindicaciones de la 1 a la 115. Reivindicación 122: El cultivo de las plantas transplastómicas de la reivindicación120. Reivindicación 124: La purificación y utilización de la proteína o proteínas que producen las células de las plantas de la reivindicación 120 como resultado de la expresión del gen o los genes en cuestión .
ARP030103474A 2002-09-27 2003-09-24 Vector para la produccion de plantas angiospermas transplastomicas AR041375A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU20020208 2002-09-27

Publications (1)

Publication Number Publication Date
AR041375A1 true AR041375A1 (es) 2005-05-11

Family

ID=32034750

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP030103474A AR041375A1 (es) 2002-09-27 2003-09-24 Vector para la produccion de plantas angiospermas transplastomicas

Country Status (12)

Country Link
US (1) US20060026704A1 (es)
EP (1) EP1557468A2 (es)
JP (1) JP2006500037A (es)
KR (1) KR20050054928A (es)
CN (1) CN1685047A (es)
AR (1) AR041375A1 (es)
AU (1) AU2003266096A1 (es)
BR (1) BR0314841A (es)
CA (1) CA2496913A1 (es)
MX (1) MXPA05003185A (es)
WO (1) WO2004029256A2 (es)
ZA (1) ZA200501740B (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2598792A1 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
US20140199313A1 (en) 2005-03-02 2014-07-17 Metanomics Gmbh Process for the Production of Fine Chemicals
JP2006246744A (ja) * 2005-03-09 2006-09-21 Research Organization Of Information & Systems 遺伝子導入方法、ジーンターゲッティング方法及びトランスジェニック植物の製造方法
KR100740694B1 (ko) * 2006-02-09 2007-07-18 한국생명공학연구원 색소체 게놈 내에 삽입된 외래유전자의 이차 재조합을방지하기 위한 벡터개발
GB0604938D0 (en) 2006-03-11 2006-04-19 Renovo Ltd Proteins, nucleic acids and medicaments
GB0604964D0 (en) 2006-03-11 2006-04-19 Renovo Ltd Protein folding
GB0604966D0 (en) 2006-03-11 2006-04-19 Renovo Ltd Medicaments and proteins
GB0617816D0 (en) * 2006-09-11 2006-10-18 Renovo Ltd Nucleic acids and methods of protein expression
WO2008112645A2 (en) 2007-03-09 2008-09-18 Monsanto Technology Llc Methods for plant transformation using spectinomycin selection
US20110037083A1 (en) * 2009-01-14 2011-02-17 Alex Chi Keung Chan Led package with contrasting face
CN102618566B (zh) * 2011-01-31 2014-01-08 中国科学院海洋研究所 一种构建亚心型扁藻叶绿体表达系统的方法
CN108192897A (zh) * 2018-02-11 2018-06-22 云南省烟草农业科学研究院 一种烟草rbcl基因片段及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0251654A2 (en) * 1986-06-23 1988-01-07 Biotechnica International, Inc. Transformation of chloroplasts
RU2192466C2 (ru) * 1995-08-10 2002-11-10 Ратгерс Юниверсити Кодируемая ядерной днк система транскрипции в пластидах высших растений
DE69841673D1 (de) * 1997-06-03 2010-07-01 State University Of New Jersey Plastid promotoren zur transgenen expression in den plastiden höherer pflanzen

Also Published As

Publication number Publication date
WO2004029256A2 (es) 2004-04-08
JP2006500037A (ja) 2006-01-05
CA2496913A1 (en) 2004-04-08
ZA200501740B (en) 2005-09-06
US20060026704A1 (en) 2006-02-02
MXPA05003185A (es) 2005-06-08
KR20050054928A (ko) 2005-06-10
BR0314841A (pt) 2005-08-09
CN1685047A (zh) 2005-10-19
WO2004029256A3 (es) 2004-04-29
EP1557468A2 (en) 2005-07-27
AU2003266096A1 (en) 2004-04-19

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