Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
  • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
  • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
  • C12Q1/6844—Nucleic acid amplification reactions
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
  • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
  • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M1/00—Apparatus for enzymology or microbiology
  • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/02—Devices for withdrawing samples
  • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/02—Devices for withdrawing samples
  • G01N1/22—Devices for withdrawing samples in the gaseous state
  • G01N1/2202—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling
  • G01N1/2211—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling with cyclones
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/02—Devices for withdrawing samples
  • G01N1/22—Devices for withdrawing samples in the gaseous state
  • G01N1/2273—Atmospheric sampling
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/02—Devices for withdrawing samples
  • G01N1/22—Devices for withdrawing samples in the gaseous state
  • G01N1/24—Suction devices
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
  • G01N1/40—Concentrating samples
  • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
  • G01N35/00584—Control arrangements for automatic analysers
  • G01N35/00722—Communications; Identification
  • G01N35/00871—Communications between instruments or with remote terminals
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
  • G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
  • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2300/00—Additional constructional details
  • B01L2300/08—Geometry, shape and general structure
  • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
  • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2400/00—Moving or stopping fluids
  • B01L2400/04—Moving fluids with specific forces or mechanical means
  • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
  • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L7/00—Heating or cooling apparatus; Heat insulating devices
  • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/02—Devices for withdrawing samples
  • G01N2001/022—Devices for withdrawing samples sampling for security purposes, e.g. contraband, warfare agents
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/02—Devices for withdrawing samples
  • G01N2001/022—Devices for withdrawing samples sampling for security purposes, e.g. contraband, warfare agents
  • G01N2001/025—Devices for withdrawing samples sampling for security purposes, e.g. contraband, warfare agents postal items
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/02—Devices for withdrawing samples
  • G01N1/22—Devices for withdrawing samples in the gaseous state
  • G01N1/2202—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling
  • G01N1/2214—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling by sorption
  • G01N2001/2217—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling by sorption using a liquid
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
  • G01N35/00584—Control arrangements for automatic analysers
  • G01N35/00722—Communications; Identification
  • G01N35/00871—Communications between instruments or with remote terminals
  • G01N2035/00881—Communications between instruments or with remote terminals network configurations
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
  • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
  • G01N35/04—Details of the conveyor system
  • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
  • G01N2035/0429—Sample carriers adapted for special purposes
  • G01N2035/0436—Sample carriers adapted for special purposes with pre-packaged reagents, i.e. test-packs

Definitions

• This invention is directed to biohazard detection systems and more particularly to a biohazard detection system for detecting biological agents, such as bacillus anthracis, in pieces of mail.
• the current state of the art in biological agent detection systems includes: (1) automated systems used, for example, by the military that utilize a form of immunoassay technology; and (2) manual systems including bio-identifier apparatus used in laboratories by skilled laboratory technicians.
• the automated immunoassay systems used by the military have not demonstrated sufficient sensitivity or specificity to be acceptable for use in civilian applications such as mail screening within the United States Postal Service (USPS).
• USPS United States Postal Service
• manual systems that require skilled technicians to perform sample preparation and to interpret test results are impractical in an industrial environment.
• a typical bio-detection system in accordance with the known prior art is comprised of the following subsystems: (a) a trigger to detect the presence of a bio-agent and start the sample collection process; (b) an aerosol collector for collecting samples from the air; and, (c) an identifier to identify the specific bio-agent.
• MPE Mail Processing Equipment
• the subject invention utilizes the polymerase chain reaction (PCR) technology that is particularly adapted for USPS application.
• PCR polymerase chain reaction
• the limit of detection for immunoassay based technology is in the range of 10,000 to
• PCR has demonstrated the ability to detect less than 200 spores per ml of sample. This difference in sensitivity is critical, and may make the difference between detecting and missing a lethal threat in the USPS application. Since PCR detects the actual DNA sequence of an agent, it is also, much less likely to cause false positives than the systems based on immunoassay techniques.
• BDS which combines automated fluidic transport apparatus with aerosol collector apparatus and biological agent identifier apparatus.
• the invention includes means for implementing the following features: particle collection and pre-separation using a collection hood or other means capable of collecting emitted particulates from items and dry cyclone passive filtration system; continuous particle collection into a liquid sample; automated fluid transfer to a sample analysis cartridge; and polymerase chain reaction (PCR) type bio-agent identifier apparatus for detecting an actual DNA sequence so as to identify a bio-agent when a collected liquid sample is manually taken from an aerosol collector, prepared, and introduced manually into the bio- agent identifier.
• PCR polymerase chain reaction
• the system also provides for automatic retesting upon various error conditions; automatic confirmation testing upon preliminary positive results; automated fluid transfer to archive containers at the completion of analysis; and automated notification/ reporting system to alert designated personnel/ organizations upon the occurrence of selected events.
• the biological agent detection system in accordance with the subject invention is not limited to, but is of particular importance to the US Postal Service (USPS) due to the fact that it would enhance the safety of its work force by quickly detecting the presence of toxic biological agents in a mail processing facility.
• the system would notify facility personnel so that appropriate actions may be taken quickly to contain a threat from biological agents, such as bacillus anthracis, in mail being processed at the facility, thereby preventing dispersion of biological agents between USPS facilities and the general public.
• optical trigger device may still be used, for example, to create a record of particle concentration spikes that occur during the mail processing window. This record will permit one to identify the contaminated machine and the approximate time the contaminated letter passed the machine after the identifier indicates that a biological agent is present.
• the subject system is compatible with the integration of a trigger that operates in parallel with the continuous collection process. In such an implementation, the trigger would be used to alert an operator to transfer a sample for analysis, resulting in a more timely response to an incident.
• Figure 1 is a system block diagram illustrative of a bio-detection system in accordance with a preferred embodiment of the subject invention
• Figures 2 A, 2B and 2C are illustrative of the location and mechanical details of two types of aerosol sampling systems located at a mail processing facility;
• Figure 3 is a system block diagram further illustrative of the apparatus located in a monitor unit shown in Figure 1 ;
• Figures 4A, and 4B are perspective views respectively illustrative of top and perspective views of a PCR sample cartridge utilized in connection with the apparatus shown in Figure 3;
• Figure 5 is a diagram illustrative of the operation performed in the sample cartridge shown in Figs. 4A and 4B;
• Figure 6 is a diagram illustrative of a flow chart of the operation of the bio-detection system in accordance with the subject invention.
• BDS biohazard detection system
• USPS United States Postal Service
• FIGs 1, 2 and 3 and the BDS 10 is comprised of a single monitor unit 12; however, more than one monitor unit can be employed depending on the needs of the particular facility. In either case, one or a plurality of the monitoring units 12 is under the control of a central site command and control unit 14 ( Figure 1).
• the monitor unit 12 can be coupled to the site command and control unit 14 either by way of a hardwired network or an RF link, as desired.
• Each monitor unit 12 includes two major sub-systems under the control of a machine control processor 20, namely: an aerosol collector/ concentrator and fluidics transfer sub-system 22 and a bio-identifier sub-system 24 which are located in a cabinet shown by reference numeral 26.
• the subject BDS 10 as shown in Figure 1 includes a sampling hood 28 or other equivalent sampling device for sampling the air around one or more specific points, in this instance a pinch point location 30 located in the mail transport path 31 of high speed automated mail processing equipment 33 (MPE) as shown in Figure 2A.
• Figure 2B shows the transport path 31 of a facer/ canceller system used for canceling letters.
• Typical mail processing equipment such as the facer/ canceller transports mail items vertically by pinching the letter between two belts 11 and 13.
• the mail processing equipment switches from a loosely held, non-singulated flow of mail pieces to a singulated flow when a singulator 15 pinches an individual mail piece and pulls it away from the non-singulated items.
• the location of the sampling hood 28 at the pinch point location 30 is based upon testing that demonstrates that particles contained in mail pieces are expelled when the mail piece is pinched by the singular 15.
• a hinged sampling hood 28 is configured to capture virtually all of the particles expelled from the envelope at this critical location.
• the sampling hood includes side channels 17 ⁇ and 172 fixed on either side of the mail path. The side channels have cut-outs 19 ⁇ and 192 to allow the mail transport belts to pass through while still capturing the majority of the particles expelled from the mail piece.
• a gasket 21 is located at the top of the side channel to interface with the hinged hood 28.
• the hinged hood 28 when in the lowered position (not shown), is the final element of a tunnel consisting of the baseplate 23 of the mail processing equipment 33, the two side channels 19 ⁇ and 192 and the hinged hood 28.
• the hinged hood 28 has been shaped to guide the particles to the entry point of the sampling hose 32 located at the downstream end of the tunnel.
• the tunnel has been sized so that the sampling volume of the aerosol concentrator (nominally 450 liters per minute) creates sufficient face velocity of the air in the tunnel so that particles in the inhalable threat region (up to 10 microns) will not settle out inside the tunnel, but remain aerosolized.
• the motion of the letters through the tunnel creates airflow through the tunnel and mixes the air so that the particles do not settle out within the tunnel and are available for sampling at the entry point to the sampling hose 32 leading to the particle separator 34 and aerosol concentrator 22 (Figure 3A).
• the hood 28 is hinged as shown in Fig. 2B to allow it to be lift ed out of the way to clear mail jams that sometimes occur at the singulator.
• Alternate sampling systems have also been designed for other pieces of mail processing equipment.
• a manifold system 35 has been designed for a flats canceller.
• Figure 2C shows the stacker area 37 of a Model 15 Flats Canceller used by the USPS in canceling flats mail.
• This manifold system creates a downward airflow in the stacker area 37 of the flats canceller. After the flats are cancelled, they are stacked or placed back into an organized group so that they can be placed into a container and transported to downstream processing. As the flat sits in the stacker, a rotating arm 39 pushes against the flats to keep space available for the next flat coming from the canceller.
• the rotating arm 39 repeatedly impacts the flats sitting in the stacker, which has been shown to cause particles in the flat mail piece to be expelled. These expelled particles are then drawn down through the perforations in the baseplate(s) 41, into the suction manifolds 43, and on through the remaining components of the system. Similar sampling hood or sampling manifold designs have been developed for other types of mail processing equipment.
• the particles are sent via a hose 32 through a dry cyclone 34, that utilizes the particle aerodynamic size to separate out larger particles, from those that are in the inhalable size range, and therefore pose the highest threat to human health.
• This cleans up the aerosol sample, and prevents large dust and fibrous particles from clogging the downstream equipment and interfering with the bio-detection process.
• the large particles are captured in a container, not shown, and disposed of. No filter media that can become clogged with dust is utilized.
• the air from the pinch point 30 can, when desired, be continuously monitored by an optional particle counter, not shown, which determines the number of particles per second in a number of size ranges passing by the air sample point.
• Such an option would provide a historical record of particle count that may be useful in assisting someone in identifying the contaminated mail sorting machine and the approximate time a contaminated letter passed through the machine in the event the monitor unit described below detects a biological agent. If a spike is detected in the counted particles with characteristics that match the target of interest, such as bacillus anthracis, the system can also use this event to automatically trigger a sample analysis process to be described hereinafter. Particle characteristics evaluated can include count, size, shape, and fluorescence signature, among others. It is also possible to use a mass spectrometer, not shown, as a trigger.
• an aerosol particle collector/ concentrator assembly 22 is preferably a SpinCon ® system and constantly draws an air sample from the sampling hood 28 and the dry cyclone particle separator 34 and impinges the sample into approximately 10 ml of liquid located in a glass collector, not shown.
• the solution is pumped out of the collector to a reservoir where it is optionally mixed with a buffer liquid by one or more buffer pumps 36.
• a fraction, nominally 2 ml, of the mixed sample is automatically pumped into a polymerase chain reaction (PCR) cartridge 38 at a fill station 40. Additional buffer and treatment solutions may also be added, when desired, to the cartridge 38 at the fill station 40.
• PCR polymerase chain reaction
• an operator then manually transfers and inserts the cartridge 38 in the door 42 of the bio-identifier apparatus 24, preferably comprising a GeneXpertTM instrument that implements a (PCR) analysis capable of determining with a high degree of reliability if any particles in the liquid sample comprise a biological agent.
• the GeneXpertTM apparatus 24 automatically processes the sample and performs a PCR analysis to determine if one or more biological agents are present. If the test result is either positive for the agent(s) under test, or non-determinate, indicating that certain internal controls included in the PCR analysis did not perform correctly, an additional test is performed using an additional fraction of the original sample and a new cartridge 38.
• the remaining sample is transferred from the reservoir into a waste bottle 44, or to archive bottles 46 for later laboratory confirmatory analysis and retention as evidence.
• the system can optionally individually archive all samples or only those that generate a positive test result.
• the bio-identifier apparatus 24 is controlled by the central site command and control system 14 ( Figure 1).
• the BDS 10 continuously collects aerosol particles from the pinch point 30 along the mail transport path 31 of the MPE as shown in Figure 1. Periodically, the liquid sample containing the particles will be analyzed using an automated PCR test by the operator manually retrieving a cartridge 38 and placing it in the bio-identigier 24. This initial analysis is termed a Preliminary, or Screening Test. If the test is negative for agents of interest, no action is necessary, and the facility operations will continue as usual. [0031] If the result of the test is a "preliminary positive", the system will automatically perform a confirmation (Reflex) -test, optionally utilizing a criteria that is independent from the Screening Test, such as a secondary gene sequence from the target organism.
• a confirmation Reflex
• the site command and control system 14 ( Figure 1) provides coordination and communication of the components in the biohazard detection system (BDS).
• the command and control system 14 is designed to: (a) provide a single user interface to the entire bio-detection system; (b) allow the user to quickly determine the status of all components associated with the system; and (c) accept input to change parameters which allow for the configuration changes
• the command and control system 14 provides an alarm when a "positive" reading has been obtained from the bio- identifier 24.
• the system 14 includes a control computer, not shown, that provides an interface to the operators and supervisors about the status of the overall system.
• This computer is furthermore networked to all sensor devices (like particle counters) and to each monitor unit. 12 where a plurality of monitor units are located at a particular site.
• the system 14 provides the higher level data collection of statistics of each component that is necessary for reports and on screen visibility.
• the system 14 also provides data about the test results from the bio-identifier 24.
• the monitor unit 12 also contains a machine control processor 20 that sends and receives commands to and from the control computer of site command and control system 14.
• the control processor 20 performs machine control functions which: (a) controls the fluid interface between the collector/ concentrator sub-system 22 and the bio-identifier sub-system 24; and (b) responds to any faults or alarms therefrom.
• Machine control functionality provided by the processor 20 has been separated from the command and control 14 because the machine control processor 20 handles time critical commands that affect the operation of the system components in the monitor unit 12.
• the preferred embodiment of this equipment comprises a proprietary SpinCon® system developed by Midwest Research Instititute (MRI).
• MRI Midwest Research Instititute
• the SpinCon® apparatus 22 is an efficient device proven to be ideally suited for a broad range of advanced air sampling requirements, including the collection of bio-aerosols, particulate matter, and soluble vapors.
• the primary sample collection component of the SpinCon® system 22 consists of a vertical glass tube, not shown, open on the top end, with a nearly tangential, vertical slit cut into the side and is called the contactor. Fluid is placed in the contactor and air is drawn through the slit and out through the open top end of the contactor.
• the slit acts like a venturi/air blast atomizer; as the air passes through the slit, it speeds up and then impacts the spinning fluid in the contactor forming a wet cyclone.
• the collection fluid then atomizes into many small droplets, greatly increasing the surface area in contact with the air. These droplets then begin to follow the air path.
• the slit is only nearly tangential so the air path is across a chord of the contactor's circular cross-section. At this time, particles in the air are picked up by the fluid. As the air and droplets reach the other side of the contactor, the droplets impinge on the wall and the fluid flow is re-formed.
• the same fluid is re-atomized over and over, thus causing the concentration of particles in the fluid to increase linearly with time.
• the spinning fluid in the contactor only covers 30 to 40 percent of the slit, which is why only 30 to 40 percent of the air is sampled that is pulled into the unit.
• the SpinCon® system 22 is very effective in collecting biologicals (sizes 1 -10 microns) as well as many types of smaller particles and even chemicals (agglomerated sizes ⁇ 1 micron.) This is due to the atomized state of the fluid at the point of collection; the massive surface area collects the larger particles, while Brownian motion, which governs the motion of small particles, enables the smaller particles to be picked up in the fluid.
• PCR polymerase chain reaction
• PCR technology has been selected for the subject invention because of its superior sensitivity and specificity.
• the limit of detection for immunoassay based technology is in the range of 10,000 to 100,000 spores per ml of sample.
• PCR has demonstrated the ability to detect less than 200 spores per ml of sample..
• This difference in sensitivity is critical, and can make the difference between detecting and missing a lethal threat, for example, in a USPS application. Since PCR detects the actual DNA sequence of an agent, it is also much less likely to cause a false positive than the systems based on immunoassay techniques. Also, sequences associated with the actual virulence properties of the organism can be targeted. This will also be critical for a USPS application, since a false positive may result in a major financial loss if it causes an unnecessary shutdown of a mail processing facility.
• PCR techniques have become recognized as one of the most reliable laboratory techniques, along with culture methods, to validate immunoassay and other field screening techniques. In recent years the development of real time PCR techniques have allowed the reaction to be performed in 30 minutes or less. This enables the use of PCR in field applications where rapid results are required.
• all current PCR methods require sample preparation to remove inhibitors (such as the humic acids from soil) from the sample that may result in a false negative and add reagents necessary to run PCR. This sample processing requires significant laboratory operations that USPS personnel could not reliably perform in the current mail processing facilities. For this reason, most PCR systems, cannot be used in the USPS application or similar industrial environments.
• the subject invention uses a PCR bio-identifier system that completely automates both sample processing and detection processing and comprises a GeneXpertTM system developed by Cepheid of Sunnyvale, California.
• This system consists of two components, a disposable multi- chamber cartridge 38 such as shown in Figures 4 A and 4B and a PCR analysis instrument 48.
• the aerosol collector 22 described previously automatically loads a liquid sample into a GeneXpertTM cartridge 38 at the fill statioan 40 ( Figure 3) which is then manually transported to the GeneXpertTM instrument 48 by an operator.
• the GeneXpertTM instrument 48 then automatically performs the entire sample preparation, PCR amplification, and results analysis with no additional intervention by the operator.
• the fluid sample and liquid reagents are automatically transported from one chamber 50 ( Figure 4B) to another within the disposable cartridge 38 as shown in Figure 5 where fluids are mixed, molecules and organisms are separated, purification is accomplished, filtering is performed, lysing is completed, all automatically with no operator intervention.
• the GeneXpertTM instrument 48 automates all fluidic processing steps. [0040] The key advantages of the GeneXpertTM bio-identifier instrument 48 utilized in the subject invention are:
• the GeneXpertTM instrument 48 incorporates an ultrasonic lysing region which actually cracks open the spore, releasing the DNA from inside the organism, in about 15 seconds. This capability does not exist with any other known DNA analysis system. Systems that do not lyse the organism cannot guarantee that the DNA from the organism is actually available for PCR detection. Such systems that do not lyse can readily report a false negative, especially for spores such as bacillus anthracis; (c) inhibitor removal - Many types of common biological samples, including common dirt, contain extraneous chemicals that impede the PCR detection reaction. The presence of these inhibiting chemicals can cause PCR reaction to fail, thereby resulting in a false negative. The GeneXpertTM instrument 48 captures the spores, then actually washes them with a PCR- compatible buffer solution to remove any potential inhibiting chemicals prior to performing the PCR reaction itself. Systems which do not remove inhibitory chemicals can easily report a false negative;
• pathogen concentration can be present in raw samples or can be released into the air at extremely low concentrations, yet still remain infectious.
• the GeneXpertTM instrument 48 actually extracts and concentrates the spores from a relatively large original sample volume (up to several mL) into a small PCR reaction tube of the cartridge 38.
• Other PCR instruments simply take a small portion of the available liquid sample and perform PCR on this small portion.
• the system routinely achieves a sensitivity at least 10 times better than competitive products which do not concentrate the sample;
• the GeneXpertTM instrument 48 has a versatile multiplexing capability in that multiple DNA targets can be detected simultaneously in the same PCR reaction tube 50 of a cartridge. Multiplexing capability is a critical feature for DNA analysis and pathogen detection. For example, with the GeneXpertTM system, a single test or analysis for up to four agents can be performed within a single disposable cartridge 38. Alternatively, a completely confirmatory test for an agent such as bacillus anthracis can be performed within a single cartridge 38. This assay would include three probes for the three different DNA segments and one probe for an internal control.
• probe check A new internal control scheme that eliminates the need for these external controls is achieved by a unique combination of an internal control and probe integrity check called probe check.
• the internal control consists of a piece of DNA whose sequence is different than the target DNA and a corresponding probe that is included in the PCR bead.
• the internal control is co-amplified along with the test reaction and is used to assure that the reagent is functional and that PCR inhibitors have been successfully removed during sample preparation.
• the biological agent detection system (BDS) in accordance with the subject invention is deployed on mail processing equipment (MPE).
• MPE mail processing equipment
• the operation of the subject bio-detection system is controlled by the machine control processor 20, and its operation is synchronized with the operation of the monitored MPE so that it is only allowed to operate when the BDS collector/ concentrator is operational.
• the flow chart shown in Figure 6 is illustrative of the operational sequence.
• the BDS Prior to collecting samples, the BDS must be initialized and prepared for data collection. The following describes the tasks involved: (1) start-up of site command and control system; (2) set collection parameters.
• the collection parameters include the setup for each run in sequential order for the tour.
• the run setup will indicate the machine ID sample number, start time, stop time, and the assay description.
• the assay description is associated with a command sequence used by the GeneXpertTM instrument 48 to perform the PCR analysis.
• the command sequences are stored locally in the machine control processor 20 ( Figure 1).
• the supervisory systeml4 will have the capability to download a new assay description and associated command sequence to the machine control processor; and, (3) powers up the BDS monitor 12.
• the system will automatically perform a communications and systems status check; rinse and prime the fluid lines; and indicate whether fluid levels are low.
• the BDS initiates the air collection process. This enables the collector/ concentrator sub-system 22 to start operation.
• An indicator 25 on the cabinet 26 ( Figure 3) provides an indication that the system is active.
• Air is then sampled from the output of the air collection hood 28 where it is routed via tube 32 which is a grounded anti- static tube to the dry cyclone pre-separator 34 that is designed to eliminate particles that are larger than the inhalation threat range of 1-10 microns.
• the sampled aerosol is routed to the dry-cyclone 34.
• the machine control processor 20 sends a signal to the collector/ concentrator
• liquid sample As the liquid sample is transferred into the reservoir 54, it is mixed with a solution containing additives that minimize PCR inhibition.
• the liquid sample is then allowed to sit in the reservoir for a time, e.g., approximately two minutes, to allow thorough mixing of the additive solution, and allow any large particles to settle to the bottom of the reservoir bottle(s) 52.
• an operator places a PCR cartridge 38 in position at the "liquid fill" station 40 in the BDS cabinet 26 as shown in Figure 3.
• the three needles at the liquid fill station 40 two of which are shown by reference numerals 56 and 58, pierce a seal on the top of the cartridge 38, and allows the sample and wash buffer solutions to be added to the appropriate cartridge chambers.
• the liquid transfers are performed utilizing the pumps 36.
• an operator takes the cartridge 38 and manually places it in the GeneXpertTM instrument 48, whereupon the sample analysis process is started.
• PCR thermal cycling analysis begins.
• the primary PCR test is called a Screening Test. This test targets one or more gene sequences for each of the organisms of interest.
• the Screening Test also includes an internal control signal that provides a built-in positive control that the PCR reaction has proceeded properly.
• the fluorescence signals in the cartridge reaction chamber are monitored and analyzed on each thermal cycle using an algorithm that analyzes the shape of the PCR growth curve, including features such as its cycle threshold and endpoint to determine whether the PCR result indicates the presence of the target organism.
• test results of the Screening Test are negative (N).
• the test results are sent to the site command and control system 14 (Fig. 1) where the results are logged.
• the test cartridge 38 is manually removed from the GeneXpertTM instrument 48.
• the remaining liquid sample in the reservoir bottle(s) 52 is transferred to one of archive bottles 46. or optionally to a waste bottle 44 if the "archive all" parameter is turned OFF.
• the SpinCon® reservoir 54 is then available for the next sample.
• a portion of the archived sample will be utilized to repeat the same assay in a new cartridge 38. If the error appears to be an inhibited sample, a portion of the archived sample will be utilized to perform a slightly modified assay. This assay will: (1) perform additional washes; (2) utilize a higher level of dilution; and (3) adjust the positive detection thresholds based on the modified dilution.
• (Reflex Test) In response to a positive (Y) Screening Test result, (a) the site command and control system 14 will send out Preliminary Positive notifications to the designated contact list, (b) an operator will manually retrieve the cartridge to be used for the Reflex Test, and transport it to the fill station 40 where a fraction of the sample remaining in the reservoir and buffer solutions are transferred into it, and depending on the agents to be tested for, the Reflex Test may simply consist of a repeat of the Screening Test, or it may be performed on a special "reflex" cartridge 38' containing primers for alternate genetic sequences, (c) the appropriate assay for the reflex cartridge is selected, and (d) the reflex cartridge 38' will then be automatically loaded into the GeneXpertTM instrument 48 and a Reflex analysis will be performed.
• the site emergency response plan is put into effect.

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